##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938406_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478041 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.717013812622767 31.0 31.0 34.0 30.0 34.0 2 31.94251539093927 31.0 31.0 34.0 30.0 34.0 3 31.988666244108767 33.0 31.0 34.0 30.0 34.0 4 35.682121407996384 37.0 35.0 37.0 33.0 37.0 5 35.53711710920193 37.0 35.0 37.0 33.0 37.0 6 35.57733960057819 37.0 35.0 37.0 33.0 37.0 7 35.515736097949755 37.0 35.0 37.0 33.0 37.0 8 35.516961934227396 37.0 35.0 37.0 33.0 37.0 9 37.07093743005307 39.0 37.0 39.0 33.0 39.0 10-11 36.98752826640393 39.0 37.0 39.0 33.0 39.0 12-13 36.902066977518665 39.0 37.0 39.0 33.0 39.0 14-15 37.890449773136616 40.0 37.0 41.0 32.5 41.0 16-17 37.830476883781934 40.0 37.0 41.0 32.5 41.0 18-19 37.95652464955934 40.0 37.0 41.0 33.0 41.0 20-21 37.98384761976483 40.0 37.0 41.0 33.0 41.0 22-23 37.889128756738444 40.0 37.0 41.0 32.5 41.0 24-25 37.737369807192266 40.0 37.0 41.0 32.0 41.0 26-27 37.489262427281346 39.0 36.0 41.0 32.0 41.0 28-29 37.35616924071367 39.0 36.0 41.0 31.5 41.0 30-31 37.091859275668824 39.0 36.0 41.0 31.0 41.0 32-33 36.80520708474796 39.0 35.0 40.5 30.5 41.0 34-35 36.57628111396303 39.0 35.0 40.0 30.0 41.0 36-37 36.48310605157299 39.0 35.0 41.0 30.0 41.0 38-39 36.29645155959426 39.0 35.0 40.0 30.0 41.0 40-41 36.118827673776934 39.0 35.0 40.0 30.0 41.0 42-43 35.83645754234469 38.0 35.0 40.0 29.0 41.0 44-45 35.51530412663349 38.0 35.0 40.0 27.5 41.0 46-47 35.08976531301708 38.0 34.0 40.0 26.5 41.0 48-49 34.6551279074389 38.0 34.0 40.0 24.5 41.0 50-51 34.2050033365339 38.0 33.0 40.0 23.5 41.0 52-53 33.706499024142275 37.0 33.0 40.0 22.5 41.0 54-55 33.36177022472968 37.0 32.5 40.0 21.5 41.0 56-57 32.90955901272066 36.5 32.0 39.5 19.5 41.0 58-59 32.380678644718756 36.0 31.0 39.0 15.5 40.5 60-61 31.855323078982764 35.5 31.0 39.0 10.5 40.0 62-63 31.36749044538021 35.0 30.5 38.5 7.5 40.0 64-65 30.823269343006146 35.0 29.5 38.0 4.0 40.0 66-67 30.232386970992028 34.5 29.0 37.5 2.0 39.5 68-69 29.643514886798414 34.0 28.5 37.0 2.0 39.0 70-71 29.040837501385866 34.0 27.5 36.0 2.0 39.0 72-73 28.625302013844 34.0 27.0 35.5 2.0 38.0 74-75 28.20422516060338 33.0 26.0 35.0 2.0 37.5 76-77 26.96832070889317 31.5 25.5 34.0 2.0 36.0 78-79 27.6740781648436 33.0 26.0 35.0 2.0 36.0 80-81 27.447131103817455 33.0 26.0 35.0 2.0 36.0 82-83 27.039486152861365 33.0 25.5 35.0 2.0 35.5 84-85 26.611037546988648 32.5 25.0 35.0 2.0 35.0 86-87 26.1824383264197 32.0 24.0 34.5 2.0 35.0 88-89 25.60910152058087 32.0 22.0 34.0 2.0 35.0 90-91 25.146136000886955 31.5 20.0 34.0 2.0 35.0 92-93 24.53265305695537 31.0 18.0 34.0 2.0 35.0 94-95 23.870231842038656 31.0 9.0 34.0 2.0 35.0 96-97 22.980647057469966 31.0 2.0 34.0 2.0 35.0 98-99 21.85341738470131 30.0 2.0 34.0 2.0 35.0 100-101 18.36007371752632 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.520141557537535 1101 2 0.23359541202058054 1101 3 0.2635825971235093 1101 4 0.21115430204958585 1101 5 0.2054255774491196 1101 6 0.08624755695718278 1101 7 0.4155780735205923 1101 8 0.4367612200712756 1101 9 0.46917298117165274 1101 10-11 0.33582465081898505 1101 12-13 0.44816152025837397 1101 14-15 0.40153794160578116 1101 16-17 0.3692122546406722 1101 18-19 0.5892788332630801 1101 20-21 0.5765105948852849 1101 22-23 0.6243461894150713 1101 24-25 0.48740619478397207 1101 26-27 0.48408813289872654 1101 28-29 0.7171922842976244 1101 30-31 0.7294583920546458 1101 32-33 0.7304185902491938 1101 34-35 0.5491452303140392 1101 36-37 0.6556668535398487 1101 38-39 0.7314375161764062 1101 40-41 0.8483960149117209 1101 42-43 0.7212848027692118 1101 44-45 0.48646957591153495 1101 46-47 0.8623299120484589 1101 48-49 0.9606020141505738 1101 50-51 0.9886306789560066 1101 52-53 1.0616671011499719 1101 54-55 0.8051203021241378 1101 56-57 0.9722788070041801 1101 58-59 0.8244492922387039 1101 60-61 1.0204014606456049 1101 62-63 1.0555397509736189 1101 64-65 1.5291703381385027 1101 66-67 1.0797774644305527 1101 68-69 1.2023270937081278 1101 70-71 0.8557627701111095 1101 72-73 0.8714623915343793 1101 74-75 1.03435191255954 1101 76-77 0.8169584599738364 1101 78-79 0.9875279502710548 1101 80-81 0.8955673124127586 1101 82-83 0.514261692137719 1101 84-85 0.44583276525144555 1101 86-87 0.7660052964266981 1101 88-89 0.762215056340434 1101 90-91 0.3354410090796769 1101 92-93 0.5281176145397026 1101 94-95 0.5910687716380849 1101 96-97 1.2034939908449758 1101 98-99 1.1303404985499732 1101 100-101 1.5089554313043045 1102 1 0.37988830250047556 1102 2 0.18683109010573062 1102 3 0.4643946810917967 1102 4 0.2795411041959497 1102 5 0.030048201631942106 1102 6 0.19474713751283446 1102 7 0.24597364180563375 1102 8 0.258003470278517 1102 9 0.37428920944865496 1102 10-11 0.5514761297946507 1102 12-13 0.2854320047786416 1102 14-15 0.48240135166350484 1102 16-17 0.4172498011146075 1102 18-19 0.5879807895118105 1102 20-21 0.7087373231697356 1102 22-23 0.4524435005037901 1102 24-25 0.4857433089075869 1102 26-27 0.5530011019194134 1102 28-29 0.4253185842028131 1102 30-31 0.42929508177007136 1102 32-33 0.5381120434426805 1102 34-35 0.7669137863830784 1102 36-37 0.7542015284332493 1102 38-39 0.6137051961279596 1102 40-41 0.35204195424368123 1102 42-43 0.21147625801773273 1102 44-45 0.29531712154162193 1102 46-47 0.037687432511887664 1102 48-49 0.034357207566536374 1102 50-51 0.12505681455814255 1102 52-53 0.26930942680755976 1102 54-55 0.38825715018600704 1102 56-57 0.16050756053471815 1102 58-59 -0.100720843323721 1102 60-61 -0.1559264902322539 1102 62-63 0.2763188750546881 1102 64-65 0.12191717741437103 1102 66-67 0.20063948461352155 1102 68-69 0.2506649524075044 1102 70-71 0.13599130523274638 1102 72-73 0.42346356300025434 1102 74-75 0.019758227554795837 1102 76-77 0.06177497168600965 1102 78-79 0.31100392777493724 1102 80-81 0.4200191805533535 1102 82-83 0.43534907058572614 1102 84-85 0.6677585360114584 1102 86-87 0.47944290345946783 1102 88-89 0.7843091012234602 1102 90-91 1.1513570606440275 1102 92-93 0.5972241732594306 1102 94-95 0.6185882919327383 1102 96-97 0.5085566096668614 1102 98-99 0.7965099540665364 1102 100-101 1.134575290629126 1103 1 0.38981996112011785 1103 2 0.3103307285728327 1103 3 0.3227297637778719 1103 4 0.1465249868260443 1103 5 0.01655719330502592 1103 6 0.29991355927012364 1103 7 0.331953162716232 1103 8 0.2386066471030901 1103 9 0.40064612405691236 1103 10-11 0.27288820732596264 1103 12-13 0.20976433514973536 1103 14-15 0.30814949151842086 1103 16-17 0.30846571789206934 1103 18-19 0.2811466514762131 1103 20-21 0.4738925490694825 1103 22-23 0.5441562334592618 1103 24-25 0.647131595241639 1103 26-27 0.5612860364936196 1103 28-29 0.6699247465894018 1103 30-31 0.23640211883302698 1103 32-33 0.48558947679233455 1103 34-35 0.30711624480679234 1103 36-37 0.25010104561189195 1103 38-39 0.480210922342593 1103 40-41 0.5201757447412021 1103 42-43 0.5597607260978705 1103 44-45 0.1574914503732856 1103 46-47 -0.01907556517897291 1103 48-49 0.18874108553168156 1103 50-51 0.5822676465503136 1103 52-53 0.4982483860593021 1103 54-55 0.5914223124093141 1103 56-57 0.6620329961235498 1103 58-59 0.6249783146544914 1103 60-61 0.7402902429098184 1103 62-63 0.5408280066759907 1103 64-65 0.6764083490142134 1103 66-67 0.4229752203912831 1103 68-69 0.2528598069779804 1103 70-71 0.037948247830655646 1103 72-73 0.1939741407531521 1103 74-75 0.24542939588918244 1103 76-77 0.2366799235205015 1103 78-79 -5.16143132159641E-4 1103 80-81 -0.02776380640813514 1103 82-83 -0.06213652641005396 1103 84-85 0.12061931085809974 1103 86-87 0.6094687938800121 1103 88-89 0.3508521469632022 1103 90-91 -0.06313924239051616 1103 92-93 -0.17510573134253704 1103 94-95 -0.6502195824870576 1103 96-97 -0.8612239048760912 1103 98-99 -0.657391102542519 1103 100-101 -1.1450319213690996 1104 1 0.33674487226422656 1104 2 0.3595636434961271 1104 3 0.27199867096282304 1104 4 0.3331538616179657 1104 5 0.2964465171004136 1104 6 0.2801763027802693 1104 7 0.1982059183737661 1104 8 0.10981733680480232 1104 9 0.35459852429086425 1104 10-11 0.6086217746089915 1104 12-13 0.31385620539763437 1104 14-15 0.5441372541494616 1104 16-17 0.449447406828952 1104 18-19 0.6031356738364124 1104 20-21 0.6658775223002422 1104 22-23 0.7186512170377952 1104 24-25 0.7962947751579605 1104 26-27 0.6500159595601573 1104 28-29 0.611830986747762 1104 30-31 0.7194002742296135 1104 32-33 0.6227402393783805 1104 34-35 0.4781064819058187 1104 36-37 0.7565998983585516 1104 38-39 0.9072343623030079 1104 40-41 1.0994779447681964 1104 42-43 0.8219306095729237 1104 44-45 0.8567648565909494 1104 46-47 0.7653487835860489 1104 48-49 0.6723826026055164 1104 50-51 0.7078244018479225 1104 52-53 0.781475992123994 1104 54-55 1.2535921958843659 1104 56-57 0.9340737573598759 1104 58-59 1.0081451837776356 1104 60-61 1.016193423725941 1104 62-63 1.1892083549011225 1104 64-65 1.2347585537202015 1104 66-67 1.0388005679415606 1104 68-69 1.1517372983173395 1104 70-71 0.6432975977204265 1104 72-73 0.6067199004148236 1104 74-75 1.0082156468452261 1104 76-77 0.3489735303950212 1104 78-79 0.43570781871604325 1104 80-81 0.7146193312172748 1104 82-83 0.5927111813327812 1104 84-85 0.8646018546111911 1104 86-87 1.023822957734783 1104 88-89 0.9943105534058212 1104 90-91 1.0493208286942028 1104 92-93 1.1407556084694086 1104 94-95 1.0788779115517215 1104 96-97 1.3698981538812731 1104 98-99 1.4630435040170724 1104 100-101 1.0810737159188797 1105 1 0.3202330907183111 1105 2 0.3552430092745915 1105 3 0.3305848854530282 1105 4 0.16483851257590487 1105 5 0.12487637836444776 1105 6 0.1929752457443712 1105 7 0.07425084239930868 1105 8 0.2210175129774541 1105 9 0.2688068484485413 1105 10-11 0.2543143533361416 1105 12-13 0.38774962094486654 1105 14-15 0.5837805045348432 1105 16-17 0.3534685475468464 1105 18-19 0.5487754007687968 1105 20-21 0.4319797024367702 1105 22-23 0.4329960749985915 1105 24-25 0.6808158057679563 1105 26-27 0.4771773777499888 1105 28-29 0.4928902248010587 1105 30-31 0.7046986666541954 1105 32-33 0.4118373545512597 1105 34-35 0.7141337886664374 1105 36-37 0.8243625057137578 1105 38-39 0.8631537633159994 1105 40-41 0.6207529943167529 1105 42-43 0.6521772512817989 1105 44-45 0.8230828482227466 1105 46-47 0.7758310562132209 1105 48-49 0.6185882841734482 1105 50-51 0.4747634020681346 1105 52-53 0.31320028192800464 1105 54-55 0.753312521803764 1105 56-57 0.5046815529996067 1105 58-59 0.5883852190121601 1105 60-61 0.5574266322703174 1105 62-63 0.6926106891887223 1105 64-65 0.5959895601064638 1105 66-67 0.6382214003573274 1105 68-69 0.6629220593831846 1105 70-71 0.5979595664497843 1105 72-73 0.5555813846693951 1105 74-75 0.2740315464268157 1105 76-77 0.4036861487610217 1105 78-79 0.5552167374564085 1105 80-81 0.44156273575371685 1105 82-83 0.5871221040370322 1105 84-85 0.6399288750912646 1105 86-87 0.9891631911635415 1105 88-89 0.9370434316264742 1105 90-91 0.8832670960361959 1105 92-93 0.8672938159126993 1105 94-95 0.6954623643154321 1105 96-97 0.49420337299600803 1105 98-99 0.5797226495797219 1105 100-101 0.35655268530888407 1106 1 0.12995509928879656 1106 2 0.17310038758466462 1106 3 0.15242424376530295 1106 4 0.11182455806541469 1106 5 -0.06855291511978123 1106 6 0.2282768609514889 1106 7 0.031802759771025535 1106 8 0.25677622081175855 1106 9 0.09694441574883683 1106 10-11 0.19407481921901848 1106 12-13 0.03368962753755511 1106 14-15 0.1956121713846386 1106 16-17 0.046935199563108654 1106 18-19 0.2199214286778286 1106 20-21 0.31486949476728654 1106 22-23 0.13805705730823092 1106 24-25 -0.10568677636587154 1106 26-27 -0.07051408472666765 1106 28-29 0.21934446059932355 1106 30-31 0.3951019190440377 1106 32-33 0.2449941505077433 1106 34-35 -0.01675292707908227 1106 36-37 -0.047636175749808274 1106 38-39 -0.028438369416669218 1106 40-41 0.2598080432124803 1106 42-43 0.416664670246746 1106 44-45 0.7871747062603234 1106 46-47 -0.10485238516685058 1106 48-49 -0.011992970633713185 1106 50-51 0.6307220879268343 1106 52-53 0.692701435647372 1106 54-55 0.5341300143870029 1106 56-57 0.7632047922040854 1106 58-59 0.9123803843786078 1106 60-61 0.37138773544578996 1106 62-63 0.6880837141310359 1106 64-65 0.36935512433017337 1106 66-67 0.49341093996835994 1106 68-69 -0.026281324860306654 1106 70-71 0.3131205377716135 1106 72-73 0.3838881641441745 1106 74-75 0.8897309201220125 1106 76-77 1.024694829125817 1106 78-79 0.6338520522569802 1106 80-81 -0.1321434503796759 1106 82-83 0.5058321359016595 1106 84-85 0.6204958457538261 1106 86-87 0.3094620594197792 1106 88-89 0.2780279388234881 1106 90-91 0.2628737326695365 1106 92-93 0.49628403676755184 1106 94-95 0.3660194459548123 1106 96-97 0.2052878692052147 1106 98-99 0.5532156723244768 1106 100-101 0.1020531797600448 1107 1 -0.04228905623322632 1107 2 -0.03158346284630653 1107 3 0.0017886374071665045 1107 4 0.013447162914623334 1107 5 -0.13036072429318324 1107 6 0.14707634006433068 1107 7 -0.009897151347438182 1107 8 0.061538617719506306 1107 9 0.12441914652045227 1107 10-11 -0.026696589863433928 1107 12-13 0.04178818269578244 1107 14-15 0.28280082761978065 1107 16-17 0.25303081956352713 1107 18-19 0.09004689633940899 1107 20-21 0.027091739956311756 1107 22-23 -0.054268125105629395 1107 24-25 0.19465634771688656 1107 26-27 0.14087722141856318 1107 28-29 0.17019090223722344 1107 30-31 0.13890398192256015 1107 32-33 0.17741317529122824 1107 34-35 0.3711426474470585 1107 36-37 0.36514416282684437 1107 38-39 0.24672854184647974 1107 40-41 0.37680302037198743 1107 42-43 0.34211992976017314 1107 44-45 0.47852839277719994 1107 46-47 0.6696820202569924 1107 48-49 0.8614335160650626 1107 50-51 0.9134397960233187 1107 52-53 0.8386614596247242 1107 54-55 0.8688310210221104 1107 56-57 0.5007107348651587 1107 58-59 0.5635389449110662 1107 60-61 0.07209051971169345 1107 62-63 0.36832720820904896 1107 64-65 0.4896841927276192 1107 66-67 0.38528758065956836 1107 68-69 0.05408412295796339 1107 70-71 0.10630766441747497 1107 72-73 0.2584370386558419 1107 74-75 0.022348378109985134 1107 76-77 0.026406315514538647 1107 78-79 0.6151125165079989 1107 80-81 0.3687122928146884 1107 82-83 0.18655931091561584 1107 84-85 0.11208103767594224 1107 86-87 0.19247741732299772 1107 88-89 -0.04664598994726177 1107 90-91 -0.3182441077157563 1107 92-93 -0.6908302070159067 1107 94-95 -0.40705978263610376 1107 96-97 -0.16258662179294348 1107 98-99 0.3951160836599712 1107 100-101 1.0589497671223285 1108 1 0.16334986703409982 1108 2 0.176476561906167 1108 3 0.21459804334776322 1108 4 0.08008933494432569 1108 5 0.010546608627606702 1108 6 -0.1968438332029976 1108 7 0.09070232266246592 1108 8 0.11445090113534917 1108 9 0.1843742826590642 1108 10-11 0.14805201780983168 1108 12-13 0.09160447291855434 1108 14-15 0.18558354729799476 1108 16-17 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86-87 -0.013555939358745661 2316 88-89 -0.11462206097692373 2316 90-91 -0.3204060447507615 2316 92-93 -0.3622744642523408 2316 94-95 -0.5954322718926797 2316 96-97 -0.9229788751674199 2316 98-99 -0.7345452381127409 2316 100-101 -0.7684246720424284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 9.0 5 29.0 6 79.0 7 172.0 8 354.0 9 688.0 10 1017.0 11 1554.0 12 2083.0 13 2696.0 14 3438.0 15 4505.0 16 5385.0 17 6580.0 18 6388.0 19 5677.0 20 5530.0 21 5664.0 22 5641.0 23 6413.0 24 7155.0 25 7646.0 26 8530.0 27 10011.0 28 11841.0 29 14253.0 30 16973.0 31 20332.0 32 24479.0 33 30103.0 34 38734.0 35 51840.0 36 67591.0 37 73074.0 38 30537.0 39 1034.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68976120995325 19.7278441955087 15.438618511196879 22.143776083341177 2 16.647516194183698 21.9533481932628 35.98593539070104 25.413200221852467 3 20.641830478266417 23.539083631251845 31.604325170122106 24.214760720359635 4 14.042728552571852 17.926495844498692 34.72254471896762 33.30823088396183 5 13.568211654302997 37.28007638047022 34.20496682432701 14.946745140899772 6 31.96253041057148 35.45177087320962 15.510594279570162 17.07510443664874 7 26.60504015345964 33.813208490485124 20.215002478866875 19.366748877188357 8 26.435180246045842 31.9916074144268 19.76587782219517 21.807334517332194 9 24.498944651191007 17.34223633537709 21.04693948845392 37.11187952497798 10-11 25.016996450095284 25.59926868197498 26.535903824148978 22.847831043780765 12-13 26.941412975037704 23.744720641116558 26.46467562405735 22.84919075978839 14-15 23.00691781667263 26.024023044048523 25.997456285130355 24.971602854148493 16-17 21.65682441464226 28.747534207316942 27.172669289872626 22.42297208816817 18-19 21.571476086779168 27.412606868448524 29.121246922335114 21.894670122437194 20-21 22.432071726065335 25.77195261494307 28.733937047240715 23.062038611750875 22-23 21.91548423670773 26.269190299576817 29.521944770427645 22.293380693287816 24-25 23.438679945862383 26.501701736880314 28.832673348101938 21.226944969155365 26-27 22.35189270438791 25.7062403963126 30.233898493012124 21.70796840628736 28-29 21.405067766153948 27.989021862141534 29.038722620026313 21.567187751678205 30-31 21.727006679343404 26.555253628872837 28.71437805543876 23.003361636344998 32-33 21.223597975905832 26.45327492830113 29.461280517779855 22.861846578013186 34-35 23.013203122221455 26.2924352448676 28.351252005782513 22.34310962712843 36-37 22.566393433192374 25.793107487754696 29.776974963418372 21.863524115634554 38-39 21.937343691327047 26.352740102150428 28.98819192900723 22.721724277515296 40-41 22.09169387121102 26.615406018018284 29.25434270299332 22.03855740777738 42-43 22.07626759657393 26.5274594934948 28.215391792118034 23.18088111781324 44-45 22.082624712106284 25.714949136161962 29.00556646814813 23.196859683583625 46-47 23.350089218288808 26.469486926853552 28.11348817360854 22.0669356812491 48-49 22.32496794208028 26.308726657336923 28.09487052365801 23.271434876924783 50-51 20.97999962346326 26.852508466846984 29.45950242761604 22.707989482073714 52-53 22.4514215307892 26.830230043029786 27.96601128355099 22.752337142630026 54-55 21.17119661284283 26.401082752316224 28.380410885258794 24.047309749582148 56-57 22.480603128183567 26.305798038243584 27.53968801839173 23.673910815181124 58-59 22.67661142035934 26.39867710091812 27.434885292265726 23.48982618645681 60-61 21.414794965285406 27.1291583776287 28.571398687560272 22.884647969525627 62-63 20.989726822594715 27.532889438353614 28.243916316801275 23.233467422250392 64-65 20.672599212201465 28.279896494233757 27.236680535769946 23.810823757794832 66-67 21.233974945637453 27.015911831738105 26.880044682406616 24.870068540217826 68-69 21.466307559553382 26.504719818005913 27.379860366603037 24.649112255837668 70-71 22.105344630841948 26.49011956099954 26.617619218455342 24.78691658970317 72-73 21.66278624636799 25.752393623141113 27.952518717013813 24.632301413477087 74-75 21.081978324034967 27.57033392533276 26.830543823646924 24.517143926985344 76-77 21.406322888622523 25.87916099246717 27.519187684738338 25.195328434171966 78-79 21.528592735769525 26.513311619714624 27.371710794680794 24.586384849835056 80-81 22.479164749098395 25.851714096846262 26.987193433130557 24.681927720924783 82-83 21.426285303081965 25.78262443022431 27.959748054029067 24.831342212664655 84-85 21.345146072821983 26.2628787799546 27.471313661705178 24.920661485518238 86-87 21.701739540634083 26.424575103785653 26.724557217822785 25.149128137757476 88-89 21.199646055463862 26.129244144330716 26.056028666997182 26.61508113320824 90-91 21.347389692653987 25.476227333963703 26.608169395934965 26.56821357744735 92-93 21.875885786725043 25.594647259460857 27.035753620808496 25.4937133330056 94-95 21.194416378511466 25.018356166102905 27.290023240684373 26.49720421470125 96-97 20.65188969146998 24.78563554172132 27.87689758828218 26.685577178526525 98-99 20.8530230670591 25.575002980915862 26.380582418662833 27.191391533362204 100-101 20.704081867454885 24.480117814162384 27.006888530481692 27.808911787901035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1074.0 1 676.0 2 302.5 3 328.5 4 282.5 5 291.0 6 458.0 7 739.5 8 1003.5 9 1112.5 10 913.5 11 659.0 12 609.5 13 586.5 14 601.5 15 673.5 16 723.0 17 756.5 18 800.0 19 860.0 20 974.5 21 1166.0 22 1337.0 23 1530.0 24 1800.0 25 2187.0 26 2635.5 27 3109.5 28 3606.5 29 4120.5 30 4572.0 31 5390.0 32 7174.0 33 7280.5 34 6174.5 35 6159.5 36 6432.0 37 7055.0 38 8149.0 39 9210.5 40 10355.0 41 11596.5 42 12529.0 43 13045.5 44 13537.5 45 15345.0 46 17300.0 47 21370.0 48 27384.0 49 26376.0 50 21784.5 51 20024.0 52 18744.0 53 18059.5 54 17707.5 55 16683.0 56 15429.0 57 14267.0 58 13276.5 59 11576.0 60 9012.0 61 7214.5 62 6131.0 63 4521.0 64 2912.5 65 2069.0 66 1584.5 67 1322.5 68 1043.5 69 759.5 70 561.0 71 409.5 72 281.0 73 242.0 74 196.0 75 141.5 76 97.5 77 66.5 78 42.5 79 23.0 80 15.0 81 13.5 82 9.5 83 3.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0012551224685748712 2 0.05146002121156972 3 4.183741561916237E-4 4 0.0 5 0.09036881773739072 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.007216954194305509 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01004097974859897 36-37 1.0459353904790593E-4 38-39 0.004288335100964143 40-41 0.005543457569539015 42-43 1.0459353904790593E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0459353904790593E-4 68-69 7.321547733353416E-4 70-71 1.0459353904790593E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0010459353904790593 82-83 4.183741561916237E-4 84-85 0.005438864030491109 86-87 0.002614838476197648 88-89 0.0 90-91 0.0027194320152455543 92-93 0.0011505289295269652 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 478041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.98187654869036 #Duplication Level Percentage of deduplicated Percentage of total 1 68.06579772639427 28.575299173376127 2 14.71587807597633 12.356003533824342 3 6.448875300025228 8.122076601706727 4 3.4603331852802697 5.810851224070911 5 2.06451482332412 4.333610322286724 6 1.3058376752461092 3.289290964488658 7 0.8540221176701634 2.509741577971594 8 0.6405217705405213 2.151224471806459 9 0.43645078484202354 1.649072067193516 >10 1.832614258426325 12.589120954606138 >50 0.08433354331751021 2.5344811794820203 >100 0.07135915203774244 6.113927817977217 >500 0.010978331082729605 3.241529780960851 >1k 0.008483255836654695 6.723770330248724 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4560 0.9538930761169021 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2996 0.6267244859750524 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2617 0.5474425833767397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2509 0.524850378942392 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2422 0.5066511031480563 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2152 0.4501705920621871 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1706 0.356873155231455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1695 0.35457209737240114 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1456 0.30457638570750206 RNA PCR Primer, Index 27 (95% over 23bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1367 0.28595873575697484 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1327 0.2775912526331423 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1318 0.27570856893028006 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1266 0.2648308408692978 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1261 0.2637849054788188 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1194 0.24976937124639936 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1152 0.24098351396637527 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1097 0.2294782246711056 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 931 0.19475316970720083 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 922 0.19287048600433854 RNA PCR Primer, Index 27 (96% over 25bp) GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 842 0.1761355197566736 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 829 0.17341608774142803 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 811 0.16965072033570341 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 808 0.169023159101416 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 775 0.1621199855242542 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 774 0.16191079844615838 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 759 0.15877299227472122 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 754 0.15772705688424216 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 732 0.1531249411661343 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 689 0.14412989680801438 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 668 0.13973696816800232 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 664 0.1389002198556191 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 608 0.12718574348225362 No Hit GTACAAAGTCCTCAGCCACATTTGTAGAACTTTGGGGGATGTTTGCTCCA 589 0.12321118899843318 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 588 0.12300200192033738 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 573 0.1198641957489002 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 573 0.1198641957489002 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 553 0.11568045418698396 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 516 0.10794053229743893 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 515 0.1077313452193431 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 483 0.10103735872027714 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.0459353904790593E-4 0.0 20-21 0.0 0.0 0.0 0.0011505289295269652 0.0 22-23 0.0 0.0 0.0 0.004288335100964143 0.0 24-25 0.0 0.0 0.0 0.010773134521934312 0.0 26-27 0.0 0.0 0.0 0.02060492719243747 0.0 28-29 0.0 0.0 0.0 0.0510416470553781 0.0 30-31 0.0 0.0 0.0 0.11913204097556486 0.0 32-33 0.0 0.0 0.0 0.2515474614102138 0.0 34-35 0.0 0.0 0.0 0.4473465665078937 0.0 36-37 0.0 0.0 0.0 0.7059017950343172 0.0 38-39 0.0 0.0 0.0 1.009641432429436 0.0 40-41 0.0 0.0 0.0 1.2857683755159077 0.0 42-43 0.0 0.0 0.0 1.564405563539529 0.0 44-45 0.0 0.0 0.0 1.9213833123100321 0.0 46-47 0.0 0.0 0.0 2.3635002018655302 0.0 48-49 0.0 0.0 0.0 2.7386772264303687 0.0 50-51 0.0 0.0 0.0 3.116887463627597 0.0 52-53 0.0 0.0 0.0 3.453678659361854 0.0 54-55 0.0 0.0 0.0 3.8557362234620043 0.0 56-57 0.0 0.0 0.0 4.3486855729947855 0.0 58-59 0.0 0.0 0.0 4.8048179967827025 0.0 60-61 0.0 0.0 0.0 5.2801956317554355 0.0 62-63 0.0 0.0 0.0 5.742708261425276 0.0 64-65 0.0 0.0 0.0 6.253229325518104 0.0 66-67 0.0 0.0 0.0 6.8531778654968925 0.0 68-69 0.0 0.0 0.0 7.529793469597797 0.0 70-71 0.0 0.0 0.0 8.187686830209124 0.0 72-73 0.0 0.0 0.0 8.858340602584295 0.0 74-75 0.0 0.0 0.0 9.558908127127172 0.0 76-77 0.0 0.0 0.0 10.291899648774896 0.0 78-79 0.0 0.0 0.0 11.179585014674473 0.0 80-81 0.0 0.0 0.0 12.050849194943531 0.0 82-83 0.0 0.0 0.0 12.924309839532594 0.0 84-85 0.0 0.0 0.0 13.835528751717948 0.0 86-87 0.0 0.0 0.0 14.724050865929911 0.0 88-89 0.0 0.0 0.0 15.675329103570615 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTA 90 5.645743E-7 36.980198 4 TCGTATC 40 1.0780041E-5 35.622173 94-95 GGATAGG 70 2.0336494E-4 33.9792 1 TAAGGTG 310 0.0 33.742302 5 AGGTAAG 285 0.0 33.38307 2 GTAAGGT 330 0.0 31.697313 4 AAGGTAA 300 0.0 30.128222 1 GGTAAGG 335 0.0 29.804937 3 AAGGTGT 415 0.0 28.612188 6 CTAAACA 85 6.3655456E-4 27.968218 4 GTATATG 90 8.8623684E-4 26.428265 1 AGGTGTG 430 0.0 25.404963 7 GTGTAAG 115 1.2673251E-4 24.819586 1 GTGTGCA 445 0.0 24.548616 9 GGTGTGC 430 0.0 24.300398 8 GTAAGCG 60 1.711443E-4 23.748117 94-95 GTACATG 1465 0.0 23.379543 1 TTGGCGC 275 5.0931703E-11 22.476276 4 TACATGG 1530 0.0 22.075375 2 GCGCTTT 280 6.548362E-11 22.051825 7 >>END_MODULE