##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938405_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 477990 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.011389359610035 31.0 31.0 34.0 28.0 34.0 2 31.13618904161175 31.0 31.0 34.0 28.0 34.0 3 31.16660599594134 31.0 31.0 34.0 27.0 34.0 4 34.81423879160652 37.0 35.0 37.0 32.0 37.0 5 34.823088349128646 37.0 35.0 37.0 32.0 37.0 6 34.853505303458235 37.0 35.0 37.0 32.0 37.0 7 34.78123600912153 37.0 35.0 37.0 32.0 37.0 8 34.745898449758364 37.0 35.0 37.0 32.0 37.0 9 36.24019330948346 39.0 37.0 39.0 32.0 39.0 10-11 36.12341576183602 39.0 36.0 39.0 32.0 39.0 12-13 35.994285445302204 39.0 35.0 39.0 31.0 39.0 14-15 36.972667838239296 39.5 36.5 41.0 31.0 41.0 16-17 36.8313019100818 39.0 36.0 41.0 31.0 41.0 18-19 36.7586162890437 39.0 36.0 41.0 31.0 41.0 20-21 36.7364202179962 39.0 36.0 41.0 30.5 41.0 22-23 36.80657231322831 39.0 36.0 41.0 31.0 41.0 24-25 36.69363375802841 39.0 36.0 41.0 30.0 41.0 26-27 36.47263018054771 39.0 36.0 41.0 30.0 41.0 28-29 36.295887989288474 39.0 35.5 41.0 30.0 41.0 30-31 36.03913157179021 39.0 35.0 40.0 29.5 41.0 32-33 35.68531768447039 38.0 35.0 40.0 28.5 41.0 34-35 35.39461704219754 38.0 35.0 40.0 27.0 41.0 36-37 35.06888114814117 38.0 34.5 40.0 25.5 41.0 38-39 34.73100169459612 38.0 34.0 40.0 25.0 41.0 40-41 34.62063641498776 38.0 34.0 40.0 24.0 41.0 42-43 34.35488713152995 38.0 33.5 40.0 23.5 41.0 44-45 34.030752735412875 38.0 33.0 40.0 22.5 41.0 46-47 33.6056873574761 38.0 33.0 40.0 19.5 41.0 48-49 33.154690474696125 37.5 33.0 40.0 16.0 41.0 50-51 32.31505784639846 36.5 31.5 39.5 13.0 40.5 52-53 32.319692880604194 37.0 31.5 39.0 10.5 40.0 54-55 32.38244733153414 37.0 31.5 40.0 7.5 41.0 56-57 32.01432456745957 36.0 31.0 39.5 4.5 41.0 58-59 31.58007071277642 36.0 31.0 39.0 2.0 41.0 60-61 31.049505219774474 35.0 30.0 39.0 2.0 40.5 62-63 30.522173058013763 35.0 29.0 38.5 2.0 40.0 64-65 30.037465218937633 35.0 28.5 38.0 2.0 40.0 66-67 29.438837632586456 34.5 28.0 37.5 2.0 40.0 68-69 28.82768467959581 34.0 26.5 37.0 2.0 39.0 70-71 28.171997322119708 34.0 26.0 36.0 2.0 39.0 72-73 27.582086445323124 33.5 25.5 35.5 2.0 38.5 74-75 26.941394171426182 33.0 24.5 35.0 2.0 37.5 76-77 26.26448565869579 32.5 22.0 35.0 2.0 37.0 78-79 25.521758823406348 32.0 19.5 35.0 2.0 36.0 80-81 24.82026925249482 31.0 16.5 35.0 2.0 36.0 82-83 24.133812422854035 31.0 8.5 34.5 2.0 35.0 84-85 23.432753823301745 30.5 3.5 34.0 2.0 35.0 86-87 22.781774723320574 30.0 2.0 34.0 2.0 35.0 88-89 22.006130881399194 29.5 2.0 34.0 2.0 35.0 90-91 21.182639804180006 29.0 2.0 34.0 2.0 35.0 92-93 20.341818866503484 28.0 2.0 34.0 2.0 35.0 94-95 19.458377790330342 26.5 2.0 34.0 2.0 35.0 96-97 18.393203832716168 24.5 2.0 33.0 2.0 35.0 98-99 17.15873763049436 20.5 2.0 33.0 2.0 35.0 100-101 14.324264105943639 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5270090311289728 1101 2 0.4807228145111573 1101 3 0.44104427282544023 1101 4 0.42624015024038897 1101 5 0.6255412601614552 1101 6 0.5685180391742293 1101 7 0.3626794694426323 1101 8 0.10659130872261358 1101 9 0.3903706534334148 1101 10-11 0.237154378363428 1101 12-13 0.2472740503090236 1101 14-15 0.6975151983829946 1101 16-17 0.6517945896594526 1101 18-19 0.8248275764964532 1101 20-21 0.7689089193494922 1101 22-23 0.7056895734998818 1101 24-25 0.588948435800944 1101 26-27 0.43627602625704753 1101 28-29 0.6111132020552006 1101 30-31 0.6383293297875738 1101 32-33 0.7972619629857718 1101 34-35 0.8534234854320317 1101 36-37 0.6677776365063792 1101 38-39 0.9375784727861998 1101 40-41 1.084470570673858 1101 42-43 0.9844641094970115 1101 44-45 0.981545910093395 1101 46-47 1.2469735955622312 1101 48-49 1.0927148327003593 1101 50-51 1.0832191345711593 1101 52-53 1.1192007255069356 1101 54-55 1.3104530105972074 1101 56-57 1.2840607440449006 1101 58-59 1.5675426171755298 1101 60-61 1.47568105642927 1101 62-63 0.9846821969957027 1101 64-65 1.2658200858715105 1101 66-67 1.5039698994641526 1101 68-69 1.8012881002482253 1101 70-71 1.3486283347075059 1101 72-73 1.4496344806804302 1101 74-75 1.8299393253922211 1101 76-77 1.7765228317035842 1101 78-79 1.775543627166261 1101 80-81 2.1387315083335245 1101 82-83 2.486491809411607 1101 84-85 2.2778151916035334 1101 86-87 2.8460533657816747 1101 88-89 3.4180464976249674 1101 90-91 3.5170883295206004 1101 92-93 3.647303818325046 1101 94-95 4.120493256861181 1101 96-97 4.627580182037729 1101 98-99 5.171431963029487 1101 100-101 5.16829231915567 1102 1 0.9393910381953638 1102 2 1.131959433189639 1102 3 1.1382124424188547 1102 4 1.0012862927283877 1102 5 0.7960997161019705 1102 6 0.9322263131815092 1102 7 1.000922363499484 1102 8 0.9447129139538504 1102 9 1.0027684807814126 1102 10-11 1.144233954379878 1102 12-13 1.0557352642519646 1102 14-15 1.3669820548930431 1102 16-17 1.4218968941471317 1102 18-19 1.473242911622755 1102 20-21 1.5509318060235486 1102 22-23 1.100595442824357 1102 24-25 1.0056757468804136 1102 26-27 1.084216752935312 1102 28-29 1.1411981042331192 1102 30-31 1.044235568251949 1102 32-33 0.9227616202130022 1102 34-35 1.032429971747483 1102 36-37 0.7498295270300304 1102 38-39 0.8730211769996714 1102 40-41 1.1228004762795152 1102 42-43 1.2715363027153828 1102 44-45 0.9760246318146386 1102 46-47 1.0848051622974495 1102 48-49 0.9694669288876696 1102 50-51 1.2152262009636061 1102 52-53 1.2825794103141916 1102 54-55 1.483729918259499 1102 56-57 1.4351338864801662 1102 58-59 1.2118051283815667 1102 60-61 1.1352866040746896 1102 62-63 1.5690201182107053 1102 64-65 1.5288190839203466 1102 66-67 1.5212641039676598 1102 68-69 1.5092720690295351 1102 70-71 1.7524911201317508 1102 72-73 1.7646848419101957 1102 74-75 1.8050962098514347 1102 76-77 1.9349365848017221 1102 78-79 1.5498514898462155 1102 80-81 2.161784295339796 1102 82-83 2.352420196270227 1102 84-85 2.7372414427241374 1102 86-87 3.230850075163101 1102 88-89 3.2711446361051664 1102 90-91 3.453841480393354 1102 92-93 3.2181233089119807 1102 94-95 3.55933468490527 1102 96-97 4.118106470072323 1102 98-99 4.313469087956978 1102 100-101 4.249260520389653 1103 1 -0.028989176131624106 1103 2 0.1105121676310219 1103 3 0.25897166687951767 1103 4 0.004209374880595362 1103 5 0.1563962972612032 1103 6 0.19488126809709172 1103 7 0.15033944653541198 1103 8 0.006850259970057948 1103 9 0.31902460492985796 1103 10-11 0.016854094513021778 1103 12-13 0.14384045636449372 1103 14-15 0.12270318702898209 1103 16-17 0.31215263955736106 1103 18-19 0.45431813971541857 1103 20-21 0.596681440638271 1103 22-23 0.25818695908824907 1103 24-25 0.28735986949448744 1103 26-27 0.7167799852232335 1103 28-29 0.5416943484124559 1103 30-31 0.6250281993657794 1103 32-33 0.4657022797030663 1103 34-35 0.40137926452669603 1103 36-37 0.5290469956020445 1103 38-39 0.6632196261152217 1103 40-41 0.9736917249988437 1103 42-43 1.0602197989547122 1103 44-45 0.667921090612289 1103 46-47 1.2598016419742635 1103 48-49 1.2092155049776352 1103 50-51 1.1647763279380285 1103 52-53 0.9175947297895917 1103 54-55 1.0513249791246722 1103 56-57 1.3111311090665652 1103 58-59 1.7038928985360222 1103 60-61 1.8331433342042835 1103 62-63 1.6387880682172558 1103 64-65 1.726566562640599 1103 66-67 1.4057003909735393 1103 68-69 1.87665785275059 1103 70-71 2.144487604664178 1103 72-73 1.7314481353789013 1103 74-75 1.9600025692545806 1103 76-77 1.8768415409312134 1103 78-79 2.0852343796188286 1103 80-81 2.4225844041056455 1103 82-83 2.4655366776950594 1103 84-85 2.441910605178556 1103 86-87 2.7167420765032517 1103 88-89 3.257252712454907 1103 90-91 3.621916276335998 1103 92-93 3.8273820016824516 1103 94-95 3.312254125343827 1103 96-97 3.790791675489249 1103 98-99 4.062928900433004 1103 100-101 4.033030131017631 1104 1 0.7817593091350652 1104 2 0.7581680593982618 1104 3 0.6336372235579226 1104 4 0.7065959980380967 1104 5 0.4692438178175067 1104 6 0.6966909347889896 1104 7 0.6701300327071493 1104 8 0.758300441827096 1104 9 0.907747242903028 1104 10-11 0.8869609828212006 1104 12-13 0.8773253001277155 1104 14-15 1.245989791043634 1104 16-17 1.1289956726744848 1104 18-19 1.262742772551185 1104 20-21 1.3300460851378233 1104 22-23 1.2570650992940102 1104 24-25 1.2616216456834621 1104 26-27 1.2188946018988744 1104 28-29 1.0873255503605677 1104 30-31 1.235520259536635 1104 32-33 1.3567238398895114 1104 34-35 1.1083913334231283 1104 36-37 1.2452543081691019 1104 38-39 1.203415150250791 1104 40-41 1.0979043085262035 1104 42-43 1.4780457949391632 1104 44-45 1.3609299452913817 1104 46-47 1.5752045098396579 1104 48-49 1.5901692638757225 1104 50-51 1.6333931905591683 1104 52-53 1.7502818065880135 1104 54-55 1.9431634491215988 1104 56-57 1.722406245181098 1104 58-59 1.8300843180893303 1104 60-61 2.048765231839031 1104 62-63 2.164675428151728 1104 64-65 2.30883850679691 1104 66-67 2.6992002819038348 1104 68-69 2.9230547685265194 1104 70-71 2.7522907627885367 1104 72-73 2.9701972617083285 1104 74-75 3.1098020145572356 1104 76-77 3.178882257318822 1104 78-79 3.0814325214272493 1104 80-81 3.7567583903138946 1104 82-83 3.982918826768856 1104 84-85 4.058944621570173 1104 86-87 4.2846212717914405 1104 88-89 4.775262568794528 1104 90-91 4.660353329762348 1104 92-93 4.719558810347483 1104 94-95 4.6894750293313265 1104 96-97 4.711482420591128 1104 98-99 5.158496218698396 1104 100-101 5.647134846835405 1105 1 0.2595911457297859 1105 2 0.018819194276201046 1105 3 0.2745614149975779 1105 4 0.2653674578404832 1105 5 -0.05470328551104586 1105 6 0.2798102952279393 1105 7 0.1963404620103617 1105 8 0.05516479229969207 1105 9 0.07560201281965817 1105 10-11 0.29408317241761495 1105 12-13 0.25996884771971907 1105 14-15 0.4422047212791824 1105 16-17 0.3653524894436728 1105 18-19 0.3346333784624065 1105 20-21 0.4366624556717227 1105 22-23 0.3454162577482691 1105 24-25 0.4691210562756538 1105 26-27 0.43567664960875874 1105 28-29 0.4324270356682405 1105 30-31 0.42394429022871094 1105 32-33 0.5265954561528758 1105 34-35 0.5682880263095171 1105 36-37 0.5779310769283441 1105 38-39 0.7904674682309363 1105 40-41 0.3948158116232747 1105 42-43 0.7153799570155925 1105 44-45 0.6945883459600921 1105 46-47 0.9952593783481305 1105 48-49 0.8983609607272172 1105 50-51 0.921519302396625 1105 52-53 0.9743553646821894 1105 54-55 0.7662789516185455 1105 56-57 1.0533726596526733 1105 58-59 0.8625917660606994 1105 60-61 1.226052669951212 1105 62-63 1.3083216120040966 1105 64-65 1.4058464584440387 1105 66-67 1.4279569529285467 1105 68-69 1.102534803676658 1105 70-71 1.5033034725641343 1105 72-73 1.520121174518838 1105 74-75 1.3386422743253341 1105 76-77 1.7379709256858398 1105 78-79 1.8061597863606984 1105 80-81 2.3105488183311245 1105 82-83 2.2418981867791423 1105 84-85 2.7418046425745253 1105 86-87 2.8851806733817718 1105 88-89 3.2120287560761795 1105 90-91 3.274904201014248 1105 92-93 3.5445346982099686 1105 94-95 3.9960506771440905 1105 96-97 4.1236842338238695 1105 98-99 4.366672912442102 1105 100-101 3.993106512394702 1106 1 0.445255533206236 1106 2 0.23788818388224797 1106 3 0.342615353480614 1106 4 0.18504823008375837 1106 5 0.2296719129478575 1106 6 0.2238353592640081 1106 7 0.30322672124531636 1106 8 0.3557723411470448 1106 9 0.19956314662858432 1106 10-11 0.25540326810126146 1106 12-13 0.08137700623280608 1106 14-15 0.5000890964776445 1106 16-17 0.11400508581531454 1106 18-19 0.45843151136107707 1106 20-21 0.451326186910741 1106 22-23 0.3274459366153053 1106 24-25 0.7096828801923323 1106 26-27 0.7716850325127211 1106 28-29 0.6387861880635128 1106 30-31 0.9281865476854705 1106 32-33 1.057610083896904 1106 34-35 1.0110090830634704 1106 36-37 1.0866759699193054 1106 38-39 0.9895148655086103 1106 40-41 1.0332289871773668 1106 42-43 1.1842277311005702 1106 44-45 1.2797933672348876 1106 46-47 1.7791879119750078 1106 48-49 1.6917287288848826 1106 50-51 0.8425548874005386 1106 52-53 1.6014389185254103 1106 54-55 1.604491022153482 1106 56-57 1.5859135686473564 1106 58-59 1.1604727542272535 1106 60-61 1.9629882528382332 1106 62-63 2.050204378362398 1106 64-65 2.285888372963818 1106 66-67 2.5989343233216573 1106 68-69 2.0505121105629165 1106 70-71 2.198618784065225 1106 72-73 2.6982663511737215 1106 74-75 2.9044247845393514 1106 76-77 2.8477467465118558 1106 78-79 3.246194503437689 1106 80-81 3.656927869538812 1106 82-83 3.632626186948496 1106 84-85 3.8315948582861203 1106 86-87 4.488229002093217 1106 88-89 3.9174543578035674 1106 90-91 4.145642362279304 1106 92-93 4.497628540162591 1106 94-95 4.556587521700493 1106 96-97 4.84315966725811 1106 98-99 5.391963456272411 1106 100-101 4.8251520679737805 1107 1 0.2586654146125973 1107 2 0.2090569388585699 1107 3 0.05998114990185144 1107 4 0.10967631540412981 1107 5 0.18453509025163584 1107 6 0.10093379617470788 1107 7 0.025185402048236938 1107 8 0.11024579377244237 1107 9 0.28054625855919113 1107 10-11 0.2316489203662755 1107 12-13 0.12999830280071478 1107 14-15 0.3064378372059693 1107 16-17 0.33776991002014967 1107 18-19 0.25498894336878664 1107 20-21 0.5287095838013229 1107 22-23 0.23801040881075153 1107 24-25 0.3632721187625094 1107 26-27 0.23024849896706456 1107 28-29 0.44006431738505114 1107 30-31 0.5518319401340293 1107 32-33 0.7521266995780849 1107 34-35 0.8218857540604105 1107 36-37 0.748128846757929 1107 38-39 0.9595291135076067 1107 40-41 0.7545465080255482 1107 42-43 1.0043027426246809 1107 44-45 1.1787457202500136 1107 46-47 1.0104287450638978 1107 48-49 1.2640247520169225 1107 50-51 1.419194454931862 1107 52-53 1.2018537867314265 1107 54-55 1.5198977187130112 1107 56-57 1.4056554284302862 1107 58-59 1.342530751964329 1107 60-61 1.239254858042944 1107 62-63 1.5802446080066197 1107 64-65 1.6940118276845162 1107 66-67 1.7323752150787683 1107 68-69 2.018682499868536 1107 70-71 1.9421736598142942 1107 72-73 1.8484716899827554 1107 74-75 2.3852549400101637 1107 76-77 2.2867506437586407 1107 78-79 2.3995113537917945 1107 80-81 2.9242559506685986 1107 82-83 3.0209009218938085 1107 84-85 3.152660943857928 1107 86-87 3.8070405513535768 1107 88-89 3.971938286898819 1107 90-91 4.005768918035077 1107 92-93 4.038191336122864 1107 94-95 4.256160081730666 1107 96-97 4.5741155403671065 1107 98-99 4.282063192265369 1107 100-101 4.6013013367161815 1108 1 0.2517771583277657 1108 2 0.46722621835818856 1108 3 0.29559720378902554 1108 4 0.3707003320834872 1108 5 0.4146261050465512 1108 6 0.4229978673827546 1108 7 0.3901386918120764 1108 8 0.23149193466495177 1108 9 0.15699467682630086 1108 10-11 0.16727499790928135 1108 12-13 0.31853324526493765 1108 14-15 0.16868073043398368 1108 16-17 0.679941434818673 1108 18-19 0.6675926882066037 1108 20-21 0.246475511390436 1108 22-23 0.204908287805182 1108 24-25 0.06115111318935362 1108 26-27 -0.06406207398510588 1108 28-29 0.12980009381742974 1108 30-31 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2.3822540499657983 2316 94-95 2.856594811835727 2316 96-97 2.947609079794713 2316 98-99 2.810423293702396 2316 100-101 2.547875824499462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5606.0 3 1114.0 4 822.0 5 775.0 6 793.0 7 874.0 8 1109.0 9 1287.0 10 1713.0 11 2160.0 12 2743.0 13 3463.0 14 4181.0 15 5015.0 16 6026.0 17 6843.0 18 6712.0 19 6123.0 20 6452.0 21 6939.0 22 7428.0 23 8086.0 24 9177.0 25 10500.0 26 11710.0 27 13262.0 28 15382.0 29 17649.0 30 20109.0 31 23258.0 32 26810.0 33 31550.0 34 37895.0 35 46214.0 36 52333.0 37 50341.0 38 24001.0 39 1535.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30733528191075 18.773277491832552 15.062097791169293 21.857289435087402 2 16.64214573178771 21.544170121572215 36.46329289297501 25.350391253665062 3 21.03261643044312 23.15590754213095 30.917797553767716 24.893678473658216 4 14.063402627194563 16.912460336729655 35.50689450198002 33.51724253409576 5 13.740873467747338 36.849839239064906 34.01956762006832 15.389719673119432 6 32.042509278233986 35.080202038385615 15.962958233817776 16.91433044956262 7 26.90906351302426 33.58537080481083 20.73229686149519 18.773268820669724 8 26.58271559464064 31.328455604435163 19.93918186422445 22.14964693669975 9 25.27015289303766 17.399248616009295 21.433489958872716 35.89710853208033 10-11 25.176421838637847 25.531986137387197 26.63205896633126 22.659533057643692 12-13 27.204749004084118 23.90512037318985 26.542479330886838 22.347651291839192 14-15 23.350639947096592 25.909381984380104 26.39133037007274 24.348647698450566 16-17 21.65853005246076 28.33289040577047 27.653641806223728 22.35493773554504 18-19 21.546925485075253 27.44086183516649 29.566257345186074 21.44595533457218 20-21 22.53633432708376 25.825861020741097 29.242213848534853 22.395590803640285 22-23 22.004612393610454 25.88287366352547 29.869538739711576 22.242975203152497 24-25 23.16051884116821 26.1286595486827 29.29145988441823 21.419361725730862 26-27 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27.132222482592393 29.077187667380826 22.469620380289236 52-53 22.669185674294827 26.996899361042416 27.645167229468203 22.688747735194553 54-55 21.47999598287701 26.412688250333716 28.319984768408673 23.7873309983806 56-57 22.48467971236147 26.23655486535512 27.878948282391242 23.399817139892168 58-59 23.0556223912293 25.73495412748062 27.625090228159554 23.584333253130524 60-61 21.59057742460904 26.308769485216814 29.105141973623233 22.995511116550915 62-63 21.55520871690155 26.414529843002043 28.846910253406087 23.183351186690324 64-65 21.15774706530347 27.208227716340044 28.005494350297262 23.628530868059226 66-67 21.73440033474554 26.13735027982635 27.616611747476334 24.511637637951775 68-69 21.924484759596325 25.755586543963727 28.173379795023184 24.14654890141676 70-71 22.526732722987163 25.981246031023662 27.223280615911893 24.268740630077282 72-73 22.268103577246148 25.46892965087509 28.35757187081196 23.905394901066803 74-75 21.851170293899493 27.417507174085205 27.365303477142838 23.366019054872467 76-77 22.318189236350854 25.68491574291441 27.759226014872095 24.237669005862635 78-79 22.586483218050272 26.61887964113701 27.570017198307788 23.224619942504926 80-81 23.519510409038602 26.08065697248666 27.112354848833558 23.28747776964118 82-83 22.69567801007683 25.978808523459602 27.93213622135552 23.39337724510805 84-85 22.654236266863055 26.65628242894922 27.505188631874937 23.18429267231279 86-87 23.370164994544815 26.626958349154943 26.679888825079423 23.32298783122082 88-89 22.90160275960489 26.605872216566627 26.027397260273972 24.46512776355451 90-91 23.262060342256845 25.990070261531944 26.312655969614372 24.435213426596842 92-93 24.243368984013166 25.98977453551764 26.659127121543257 23.107729358925937 94-95 23.664474358786563 25.43425901318942 26.841088718368304 24.06017790965571 96-97 23.348228251100902 25.239718897225067 27.256757901961382 24.15529494971265 98-99 23.868025593920397 25.960399727154414 25.798763825362087 24.372810853563102 100-101 24.31337725820031 24.784599210022577 26.317012997348595 24.585010534428523 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1106.0 1 696.5 2 332.0 3 357.0 4 290.5 5 277.5 6 438.5 7 783.5 8 1086.0 9 1157.0 10 963.5 11 716.0 12 625.5 13 644.5 14 700.0 15 742.5 16 762.0 17 817.5 18 924.0 19 1041.5 20 1164.0 21 1329.5 22 1577.5 23 1826.0 24 2108.5 25 2522.5 26 3027.5 27 3508.5 28 3995.5 29 4459.0 30 4819.5 31 5621.0 32 7043.5 33 7002.0 34 6089.5 35 6046.5 36 6243.5 37 6898.5 38 8097.5 39 9165.0 40 10139.5 41 11258.5 42 12188.0 43 12640.5 44 13196.5 45 14978.5 46 17081.0 47 20706.0 48 26235.5 49 26414.5 50 21556.0 51 18567.0 52 17555.5 53 17229.0 54 17246.0 55 16577.0 56 15292.5 57 14187.5 58 13397.5 59 11754.5 60 9242.0 61 7556.0 62 6614.5 63 5118.0 64 3477.5 65 2538.0 66 1921.0 67 1546.0 68 1258.0 69 983.5 70 736.5 71 546.5 72 421.5 73 347.0 74 266.5 75 195.0 76 156.0 77 124.0 78 97.0 79 67.5 80 41.0 81 23.0 82 14.5 83 13.0 84 10.0 85 7.0 86 4.5 87 2.0 88 2.0 89 0.5 90 0.5 91 1.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29352078495366013 2 0.03493796941358606 3 0.040586623151111945 4 0.04560764869557941 5 0.05481286219376975 6 0.054185234000711305 7 0.031590619050607754 8 0.035984016402016775 9 0.04288792652565954 10-11 0.03305508483441076 12-13 0.03420573652168455 14-15 0.028870896880687877 16-17 0.022803824347789704 18-19 0.026360384108454153 20-21 0.035670202305487564 22-23 0.030753781459863176 24-25 0.048745789660871564 26-27 0.04173727483838574 28-29 0.03493796941358606 30-31 0.03556559760664449 32-33 0.029498525073746312 34-35 0.030649176761020105 36-37 0.03085838615870625 38-39 0.018724241092909894 40-41 0.019351869285968326 42-43 0.02008410217786983 44-45 0.010146655787777986 46-47 0.01809661289985146 48-49 0.01600451892299002 50-51 0.007949957112073474 52-53 0.005021025544467457 54-55 0.007531538316701187 56-57 0.006067072532898178 58-59 0.007322328919015042 60-61 0.006590096027113538 62-63 0.004602606749095169 64-65 0.00930981819703341 66-67 0.0031381409652921606 68-69 0.0070085148224858255 70-71 0.01349400615075629 72-73 0.01391242494612858 74-75 0.011401912173894852 76-77 0.010042051088934915 78-79 0.007531538316701187 80-81 0.008368375907445762 82-83 0.012970982656540932 84-85 0.004602606749095169 86-87 9.414422895876484E-4 88-89 0.019979497479026758 90-91 0.027720245193414088 92-93 0.013703215548442437 94-95 0.022385405552417414 96-97 0.01809661289985146 98-99 0.013389401451913219 100-101 0.10627837402456118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 477990.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.37171852219361 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00212189184863 32.21488288955437 2 13.56358222799211 12.308060700021715 3 5.9211889772794235 8.059635587815125 4 3.1000580602547307 5.626198468493407 5 1.8203752519616718 4.129677676838612 6 1.1457744358848267 3.119145311693492 7 0.7818320969487458 2.483114608406242 8 0.5584630068002681 2.027074107967973 9 0.4105073954448723 1.6762883397663213 >10 1.5423810747261375 11.501127100213566 >50 0.0773207891676298 2.467908045475384 >100 0.05926383840268551 5.495023719146555 >500 0.010185972225461571 3.0773225193737246 >1k 0.006944981062814708 5.8145409252334845 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4425 0.9257515847611874 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2856 0.5975020397916275 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2836 0.5933178518379045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2501 0.5232327036130463 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2003 0.4190464235653465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1801 0.37678612523274546 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1491 0.31193121195004075 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1316 0.27531956735496554 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1300 0.27197221699198726 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1270 0.26569593506140293 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1259 0.2633946316868554 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1204 0.25188811481411744 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1169 0.2445657858951024 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1150 0.24059080733906568 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1098 0.22971191865938617 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 861 0.18012929140777004 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 839 0.17552668465867488 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 806 0.1686227745350321 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 788 0.16485700537668152 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 778 0.16276491139982008 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 773 0.16171886441138936 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 736 0.15397811669700204 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 726 0.1518860227201406 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 723 0.15125839452708215 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 708 0.14812025356178998 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 682 0.14268080922195026 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 682 0.14268080922195026 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 652 0.13640452729136593 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 647 0.1353584803029352 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 560 0.11715726270424068 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.1144375405343208 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 539 0.11276386535283166 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 535 0.11192702776208706 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 519 0.10857967739910877 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 517 0.10816125860373647 No Hit GTACAAAGTCCTCAGCCACATTTGTAGAACTTTGGGGGATGTTTGCTCCA 516 0.10795204920605032 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 515 0.10774283980836419 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 498 0.10418628004769974 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.230234942153601E-4 0.0 22-23 1.0460469884307202E-4 0.0 0.0 0.004707211447938241 0.0 24-25 2.0920939768614405E-4 0.0 0.0 0.01140191217389485 0.0 26-27 2.0920939768614405E-4 0.0 0.0 0.020816335069771333 0.0 28-29 2.0920939768614405E-4 0.0 0.0 0.05094248833657608 0.0 30-31 2.0920939768614405E-4 0.0 0.0 0.11715726270424068 0.0 32-33 2.0920939768614405E-4 0.0 0.0 0.23849871336220424 0.0 34-35 2.0920939768614405E-4 0.0 0.0 0.4261595430866755 0.0 36-37 2.0920939768614405E-4 0.0 0.0 0.6851607774221218 0.0 38-39 2.0920939768614405E-4 0.0 0.0 0.9806690516538004 0.0 40-41 2.0920939768614405E-4 0.0 0.0 1.2611142492520764 0.0 42-43 2.0920939768614405E-4 0.0 0.0 1.543756145526057 0.0 44-45 2.0920939768614405E-4 0.0 0.0 1.893763467854976 0.0 46-47 2.0920939768614405E-4 0.0 0.0 2.3038138873198184 0.0 48-49 2.0920939768614405E-4 0.0 0.0 2.6654323312203183 0.0 50-51 2.0920939768614405E-4 0.0 0.0 3.0365698027155377 0.0 52-53 2.0920939768614405E-4 0.0 0.0 3.374966003472876 0.0 54-55 2.0920939768614405E-4 0.0 0.0 3.7776940940187034 0.0 56-57 2.0920939768614405E-4 0.0 0.0 4.2689177597857695 0.0 58-59 2.0920939768614405E-4 0.0 0.0 4.737337601205046 0.0 60-61 2.0920939768614405E-4 0.0 0.0 5.222808008535743 0.0 62-63 2.0920939768614405E-4 0.0 0.0 5.67794305320195 0.0 64-65 2.0920939768614405E-4 0.0 0.0 6.176279838490345 0.0 66-67 2.0920939768614405E-4 0.0 0.0 6.767191782254859 0.0 68-69 2.0920939768614405E-4 0.0 0.0 7.4367664595493626 0.0 70-71 2.0920939768614405E-4 0.0 0.0 8.10749178853114 0.0 72-73 2.0920939768614405E-4 0.0 0.0 8.748404778342643 0.0 74-75 2.0920939768614405E-4 0.0 0.0 9.450302307579657 0.0 76-77 2.0920939768614405E-4 0.0 0.0 10.156070210673864 0.0 78-79 2.0920939768614405E-4 0.0 0.0 11.018954371430365 0.0 80-81 2.0920939768614405E-4 0.0 0.0 11.84721437686981 0.0 82-83 2.0920939768614405E-4 0.0 0.0 12.684261177012072 0.0 84-85 2.0920939768614405E-4 0.0 0.0 13.545994686081299 0.0 86-87 2.0920939768614405E-4 1.0460469884307202E-4 0.0 14.40071968032804 0.0 88-89 2.0920939768614405E-4 2.0920939768614405E-4 0.0 15.316952237494508 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 45 9.5972116E-4 42.24205 6 GTTAGGC 50 0.0016103205 38.021828 3 TAAGGTA 125 5.6061253E-9 34.219643 4 CCGTACT 35 0.0083930865 27.14139 16-17 GTGTAAG 145 7.633753E-7 26.299074 1 GTCTAGT 140 1.7290133E-5 23.833534 1 AAGGTAA 195 4.0707346E-7 22.000185 1 GTAAGCG 65 2.9285694E-4 21.936817 94-95 GCCTTAC 110 0.0028169355 21.66685 1 CATTGCG 55 0.0028779157 21.589745 28-29 CCTAGAG 155 3.8830378E-5 21.463934 2 TGTTCGC 70 4.8492572E-4 20.358173 10-11 AGTACTT 2525 0.0 20.223799 12-13 AGAGTAC 3535 0.0 19.215967 10-11 GAGTACT 2475 0.0 19.09693 12-13 TTACAGT 175 9.877674E-5 19.010912 4 TACTTTT 2740 0.0 18.636892 14-15 CGCAGAA 205 1.5841044E-5 18.539465 9 TTGGAGT 130 0.0075318348 18.279726 4 CTTACAG 185 1.5123178E-4 17.983297 3 >>END_MODULE