##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938405_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 477990 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.733603213456348 31.0 31.0 34.0 30.0 34.0 2 31.96849724889642 31.0 31.0 34.0 30.0 34.0 3 32.01503169522375 33.0 31.0 34.0 30.0 34.0 4 35.70829306052428 37.0 35.0 37.0 33.0 37.0 5 35.56276072721187 37.0 35.0 37.0 33.0 37.0 6 35.612224105106804 37.0 35.0 37.0 33.0 37.0 7 35.543917236762276 37.0 35.0 37.0 33.0 37.0 8 35.54676039247683 37.0 35.0 37.0 33.0 37.0 9 37.07789702713446 39.0 37.0 39.0 33.0 39.0 10-11 37.02515638402477 39.0 37.0 39.0 33.0 39.0 12-13 36.930746459130944 39.0 37.0 39.0 33.0 39.0 14-15 37.92657168560012 40.0 37.0 41.0 33.0 41.0 16-17 37.873970166739895 40.0 37.0 41.0 32.5 41.0 18-19 37.90876064352811 40.0 37.0 41.0 33.0 41.0 20-21 37.89157095336722 40.0 37.0 41.0 32.5 41.0 22-23 37.899236385698444 40.0 37.0 41.0 32.5 41.0 24-25 37.76743864934413 40.0 37.0 41.0 32.0 41.0 26-27 37.55044980020502 39.5 36.5 41.0 32.0 41.0 28-29 37.40666018117534 39.0 36.0 41.0 32.0 41.0 30-31 37.13579154375614 39.0 36.0 41.0 31.0 41.0 32-33 36.841965313081865 39.0 35.0 40.5 31.0 41.0 34-35 36.602609887236135 39.0 35.0 40.0 30.0 41.0 36-37 36.521226385489236 39.0 35.0 40.5 30.0 41.0 38-39 36.30865603882926 39.0 35.0 40.0 30.0 41.0 40-41 36.1340781187891 39.0 35.0 40.0 30.0 41.0 42-43 35.844690265486726 38.0 35.0 40.0 29.0 41.0 44-45 35.51390824075818 38.0 35.0 40.0 28.0 41.0 46-47 35.09435553045043 38.0 34.0 40.0 26.5 41.0 48-49 34.617996192388965 38.0 34.0 40.0 24.5 41.0 50-51 34.17460093307391 38.0 33.0 40.0 23.5 41.0 52-53 33.667769200192474 37.0 33.0 40.0 22.5 41.0 54-55 33.29744555325425 37.0 32.5 40.0 20.5 41.0 56-57 32.82864599677818 36.0 32.0 39.5 18.5 41.0 58-59 32.30098014602816 36.0 31.0 39.0 15.5 40.0 60-61 31.748767756647627 35.0 31.0 39.0 10.0 40.0 62-63 31.238105399694554 35.0 30.0 38.5 7.5 40.0 64-65 30.696161007552462 35.0 29.5 38.0 2.0 40.0 66-67 30.105458273185633 34.5 29.0 37.0 2.0 39.5 68-69 29.49867884265361 34.0 28.0 36.5 2.0 39.0 70-71 28.899078432603194 34.0 27.5 36.0 2.0 39.0 72-73 28.351674721228477 33.5 26.0 35.5 2.0 38.0 74-75 27.79985878365656 33.0 26.0 35.0 2.0 37.0 76-77 26.554342141048977 31.5 24.5 34.0 2.0 36.0 78-79 27.213919747275046 32.5 26.0 35.0 2.0 36.0 80-81 26.98663361158183 33.0 25.0 35.0 2.0 36.0 82-83 26.591836649302287 32.5 24.5 35.0 2.0 35.5 84-85 26.088704784618926 32.0 23.5 35.0 2.0 35.0 86-87 25.58837423377058 32.0 21.5 34.5 2.0 35.0 88-89 24.918553735433797 31.0 19.0 34.0 2.0 35.0 90-91 24.44568819431369 31.0 17.5 34.0 2.0 35.0 92-93 23.77644406786753 31.0 8.5 34.0 2.0 35.0 94-95 23.082513232494406 30.5 2.0 34.0 2.0 35.0 96-97 22.18190024895918 30.0 2.0 34.0 2.0 35.0 98-99 21.11643026004728 29.5 2.0 34.0 2.0 35.0 100-101 18.03354358877801 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4347593531325522 1101 2 0.4648174256323685 1101 3 0.2191612660479194 1101 4 0.25333240878052976 1101 5 0.13137755611769109 1101 6 0.3813503341130655 1101 7 0.3584522310912135 1101 8 0.256697961822816 1101 9 0.23032106621924697 1101 10-11 0.40047796636527266 1101 12-13 0.526448445171873 1101 14-15 0.45065765488595133 1101 16-17 0.704925105408357 1101 18-19 0.5664780997494958 1101 20-21 0.5923926597573868 1101 22-23 0.6493256490644583 1101 24-25 0.7197448267315281 1101 26-27 0.637304764634969 1101 28-29 0.43600090464457253 1101 30-31 0.5338023445173832 1101 32-33 0.29022914396169597 1101 34-35 0.3728123621860533 1101 36-37 0.68984802011461 1101 38-39 0.8952923465729441 1101 40-41 0.724581824461545 1101 42-43 0.43184782232789587 1101 44-45 0.6136687128141958 1101 46-47 0.4812473456976605 1101 48-49 0.6091639283164909 1101 50-51 0.9412120867534028 1101 52-53 0.959187119844465 1101 54-55 0.8592218675795138 1101 56-57 1.173743296811601 1101 58-59 0.8431869476514819 1101 60-61 0.7011958540222096 1101 62-63 1.0883280774704147 1101 64-65 0.6490289852950575 1101 66-67 0.7322184874225215 1101 68-69 0.781899841634484 1101 70-71 1.025382462595143 1101 72-73 1.1644345495196475 1101 74-75 1.400919117046982 1101 76-77 1.1461401836942855 1101 78-79 1.2530132077992455 1101 80-81 1.5637778803974776 1101 82-83 1.9344881885553527 1101 84-85 1.9843313476028719 1101 86-87 2.253046091743407 1101 88-89 2.355478953376224 1101 90-91 2.312223983941511 1101 92-93 2.4315149929821516 1101 94-95 2.608922473475136 1101 96-97 2.933482824536025 1101 98-99 2.6948314007673986 1101 100-101 2.806059595475425 1102 1 0.31249494349272666 1102 2 0.3113448738209499 1102 3 0.3289487469317436 1102 4 0.12791448967490027 1102 5 0.23246915554815928 1102 6 0.2661101295040922 1102 7 0.3108279100449707 1102 8 0.33680659442314465 1102 9 0.4613393650116322 1102 10-11 0.5793499802871196 1102 12-13 0.5673201426880894 1102 14-15 0.4980947692665936 1102 16-17 0.5353554170678976 1102 18-19 0.5741799401755827 1102 20-21 0.5125898859346734 1102 22-23 0.38646376012282957 1102 24-25 0.4132716948431181 1102 26-27 0.6567347071546052 1102 28-29 0.5166948876641371 1102 30-31 0.5723326403566489 1102 32-33 0.5702534017268235 1102 34-35 0.6250061079013989 1102 36-37 0.4902857145722379 1102 38-39 0.3698955211761188 1102 40-41 0.41641855915541726 1102 42-43 0.6623440518274535 1102 44-45 0.6848151558875486 1102 46-47 0.5586691671394135 1102 48-49 0.6610992760990229 1102 50-51 0.5546830565366676 1102 52-53 0.6370809130206538 1102 54-55 0.6261627524605942 1102 56-57 0.961258457914802 1102 58-59 0.986777096889476 1102 60-61 0.8607934915268203 1102 62-63 1.0981999331894485 1102 64-65 1.005259539532254 1102 66-67 0.8671839464743591 1102 68-69 1.2834528659449838 1102 70-71 0.8874006031847124 1102 72-73 0.833076114672842 1102 74-75 1.065210104759899 1102 76-77 1.1934771029581128 1102 78-79 1.3867077708958035 1102 80-81 1.7230327451340735 1102 82-83 1.8745425048555155 1102 84-85 1.9860768520061853 1102 86-87 1.9971187595702276 1102 88-89 2.1502477135934903 1102 90-91 2.2563019251955296 1102 92-93 2.4162906086547764 1102 94-95 2.408687805964721 1102 96-97 2.590301365906061 1102 98-99 2.8172118258056287 1102 100-101 3.2875604128566422 1103 1 -0.009466532032533337 1103 2 0.0071161159366361915 1103 3 -0.10351688743049436 1103 4 0.11697963750419405 1103 5 0.03083475446438655 1103 6 0.10212619188907013 1103 7 0.08726553720146768 1103 8 0.055049637536178864 1103 9 0.07936244862052177 1103 10-11 0.15522872578960545 1103 12-13 0.1554933134553238 1103 14-15 0.120412647167214 1103 16-17 0.0697550441564303 1103 18-19 0.11108992186990463 1103 20-21 0.23164867290414293 1103 22-23 0.1484267894459137 1103 24-25 -0.10539087368179878 1103 26-27 -0.2813173159387148 1103 28-29 -0.22281090751617683 1103 30-31 0.05574099299106905 1103 32-33 0.12518258253547287 1103 34-35 0.15168044644480716 1103 36-37 0.3270374031134651 1103 38-39 0.48758356712968975 1103 40-41 0.4188002398790971 1103 42-43 0.37987331906609967 1103 44-45 0.4659942942095441 1103 46-47 0.5221840520369838 1103 48-49 0.7756870098363322 1103 50-51 0.4546451827147635 1103 52-53 0.8167290749263785 1103 54-55 0.6744203138720266 1103 56-57 0.5334639282542568 1103 58-59 0.922505706677434 1103 60-61 0.9795012173009354 1103 62-63 0.9104186108103889 1103 64-65 0.6748444327329324 1103 66-67 0.8638578480121346 1103 68-69 0.9772163099378552 1103 70-71 0.7845901713745853 1103 72-73 0.7131447731140916 1103 74-75 0.2516561321358708 1103 76-77 0.5081017393937017 1103 78-79 0.8263038805745069 1103 80-81 0.9312222307784381 1103 82-83 1.1469402576754142 1103 84-85 1.36811203581561 1103 86-87 1.6913434872914372 1103 88-89 1.7560516340196166 1103 90-91 1.5696738121373137 1103 92-93 2.05942694955802 1103 94-95 2.1334058159381577 1103 96-97 2.393830914770575 1103 98-99 2.4124450654255334 1103 100-101 2.8653644109726635 1104 1 0.3996338877745238 1104 2 0.3760343670818713 1104 3 0.3626559234960638 1104 4 0.05001084034267933 1104 5 0.2885597830673561 1104 6 0.28744229369339536 1104 7 0.35143680779672337 1104 8 0.2853736858473752 1104 9 0.16905335843991764 1104 10-11 0.4079454517397352 1104 12-13 0.3932496829033454 1104 14-15 0.5028043454047335 1104 16-17 0.6433503822057247 1104 18-19 0.7054352142879949 1104 20-21 0.5568900730920063 1104 22-23 0.7689577140453636 1104 24-25 0.6419925664211235 1104 26-27 0.6436360511988042 1104 28-29 0.440893811863063 1104 30-31 0.6610155147538066 1104 32-33 0.5106081992244711 1104 34-35 0.6753240867908019 1104 36-37 0.9671385485679238 1104 38-39 1.0809472322278282 1104 40-41 1.2341452340249432 1104 42-43 1.4026644350049722 1104 44-45 1.374518202733448 1104 46-47 1.4657104313235365 1104 48-49 1.5176080611792315 1104 50-51 1.7053541357791815 1104 52-53 1.822258241055124 1104 54-55 1.6577037125729888 1104 56-57 1.6225249048955348 1104 58-59 1.7985802658355041 1104 60-61 1.6826758134091477 1104 62-63 1.8054000689610064 1104 64-65 1.9956518156388228 1104 66-67 2.0631593567354862 1104 68-69 2.1504368043574935 1104 70-71 2.2606215478342264 1104 72-73 2.0224798040765286 1104 74-75 1.8870405694475068 1104 76-77 1.9298586964825653 1104 78-79 2.6073742772765947 1104 80-81 2.263891500278543 1104 82-83 2.3477865495281307 1104 84-85 2.474835626479244 1104 86-87 2.7050283960512935 1104 88-89 2.6712310202910814 1104 90-91 2.904693645015822 1104 92-93 2.8484661122588513 1104 94-95 2.943929484063542 1104 96-97 3.4995394894298997 1104 98-99 4.006957301265391 1104 100-101 4.474540956980043 1105 1 0.22116549074942782 1105 2 0.059854586984563696 1105 3 -0.13324822807819103 1105 4 -0.04263978014299141 1105 5 0.10644348754900079 1105 6 0.20175311522071127 1105 7 0.10069629726239526 1105 8 0.2600784461191452 1105 9 0.1301052906258704 1105 10-11 0.19823629770068152 1105 12-13 0.17618471944662417 1105 14-15 0.24055216459585438 1105 16-17 0.08141755326258959 1105 18-19 0.02726687941863304 1105 20-21 0.16359860557373906 1105 22-23 0.25940716428862487 1105 24-25 0.11505770134193227 1105 26-27 0.27203264763664237 1105 28-29 0.30080550785722693 1105 30-31 0.43784214312682934 1105 32-33 0.3365249962752941 1105 34-35 0.41835299992280994 1105 36-37 0.5794668165663026 1105 38-39 0.21900368677913207 1105 40-41 0.3503550203378225 1105 42-43 0.5045162471660589 1105 44-45 0.4216643973023295 1105 46-47 0.4992166576132675 1105 48-49 0.4937359733896187 1105 50-51 0.2746213411288565 1105 52-53 0.6854877671712458 1105 54-55 0.5893345503893883 1105 56-57 0.6283225344045107 1105 58-59 0.6578596880015226 1105 60-61 0.5937995461823675 1105 62-63 1.0424877514095172 1105 64-65 0.7809637731157224 1105 66-67 0.992216089915928 1105 68-69 1.1301976119533528 1105 70-71 0.8403309162033885 1105 72-73 0.43114517047385803 1105 74-75 0.7478503086077595 1105 76-77 0.7232080331308737 1105 78-79 0.7701194173413235 1105 80-81 0.9939984510040496 1105 82-83 0.9207145131777459 1105 84-85 0.7932980222612258 1105 86-87 1.1090878083581295 1105 88-89 1.1316956879722433 1105 90-91 1.10231029417886 1105 92-93 1.2396185652669764 1105 94-95 1.2624947582989172 1105 96-97 1.5031423785998008 1105 98-99 2.321323848621631 1105 100-101 3.004429290992089 1106 1 0.2541805842103386 1106 2 0.3308171391131012 1106 3 0.286125594398996 1106 4 0.346923927810181 1106 5 0.2742074114254578 1106 6 0.28879983509200713 1106 7 0.3629076057151579 1106 8 0.3143217620233827 1106 9 0.2751422304425475 1106 10-11 0.5156181220407419 1106 12-13 0.2342871348237523 1106 14-15 0.5088855332107656 1106 16-17 0.4337895340411819 1106 18-19 0.5265492520344779 1106 20-21 0.7055152422510602 1106 22-23 0.6859022213389423 1106 24-25 0.5878958223859883 1106 26-27 0.7081299401280106 1106 28-29 0.4374764788769383 1106 30-31 0.5822503651467699 1106 32-33 0.5082559812665082 1106 34-35 0.20264140568923494 1106 36-37 0.5389430967585085 1106 38-39 0.4755798209055868 1106 40-41 0.4334113932500401 1106 42-43 0.5655950168267267 1106 44-45 0.08714419154014053 1106 46-47 -0.3451195899787649 1106 48-49 0.2437539669965929 1106 50-51 0.4700192115325521 1106 52-53 1.074310561895949 1106 54-55 0.8435588142391737 1106 56-57 0.7333416923037035 1106 58-59 0.9744032219459342 1106 60-61 0.7629479193486048 1106 62-63 1.2293862886351121 1106 64-65 0.8797677274754676 1106 66-67 0.8914730597925136 1106 68-69 0.8301616247393966 1106 70-71 1.0193249677286111 1106 72-73 1.4507222148651415 1106 74-75 1.698619799917907 1106 76-77 1.7571926644736173 1106 78-79 2.05788164770183 1106 80-81 1.8617412059311214 1106 82-83 2.096359636910254 1106 84-85 2.1564195955375425 1106 86-87 2.332242405195487 1106 88-89 2.565970333921566 1106 90-91 2.455548084987303 1106 92-93 2.7941815323069186 1106 94-95 2.9470223731933913 1106 96-97 3.2313076657565993 1106 98-99 3.7003898745747605 1106 100-101 4.059743755735205 1107 1 0.28074891174669503 1107 2 0.241276846610063 1107 3 0.15162448389319394 1107 4 0.19979657584269717 1107 5 0.2651222167726459 1107 6 0.10987430183968883 1107 7 0.23690500755063937 1107 8 0.21934627696282405 1107 9 0.38129401353771186 1107 10-11 0.38173093266807 1107 12-13 0.3180004147969271 1107 14-15 0.5297274223278166 1107 16-17 0.43195405652368635 1107 18-19 0.5076266521937285 1107 20-21 0.33662616711382043 1107 22-23 0.5160555968575338 1107 24-25 0.3225687469973053 1107 26-27 0.3556009203065358 1107 28-29 0.39957470625824243 1107 30-31 0.2859380825335194 1107 32-33 0.345876984493259 1107 34-35 0.5089063346587608 1107 36-37 0.4170430841867514 1107 38-39 0.5401903057566102 1107 40-41 0.32968386527786464 1107 42-43 0.4608701289249524 1107 44-45 0.8802800084952551 1107 46-47 0.9431362873208187 1107 48-49 0.560872518729397 1107 50-51 0.7458395191217093 1107 52-53 0.7495525683494506 1107 54-55 0.6653917774039044 1107 56-57 0.8025282991846439 1107 58-59 0.5004959177511523 1107 60-61 0.6365304529780715 1107 62-63 0.9696285046180151 1107 64-65 0.5907470678769258 1107 66-67 0.9347576426194841 1107 68-69 0.7214347253554116 1107 70-71 0.6845661360645288 1107 72-73 0.868563643017513 1107 74-75 1.0510377691589916 1107 76-77 1.1852004589671843 1107 78-79 1.3534878162472772 1107 80-81 1.5199715206442797 1107 82-83 1.6994858155131105 1107 84-85 1.738911319126366 1107 86-87 1.6462117300917676 1107 88-89 2.5214495276524467 1107 90-91 2.504487866238616 1107 92-93 2.2767214476570423 1107 94-95 2.4857853116336557 1107 96-97 2.631797964545516 1107 98-99 2.567778719229665 1107 100-101 2.8663585987976887 1108 1 0.20926019838315568 1108 2 0.2160533190851055 1108 3 0.15323628775029619 1108 4 0.2119973697162436 1108 5 0.06599627956082799 1108 6 0.057747282073044914 1108 7 0.11712632987131144 1108 8 0.21881702793511693 1108 9 0.2452248904071226 1108 10-11 0.21057299994685508 1108 12-13 0.17824860051523217 1108 14-15 0.3685289940370424 1108 16-17 0.4689654033020858 1108 18-19 0.6644106624579464 1108 20-21 0.43562380153103675 1108 22-23 0.46360581537322076 1108 24-25 0.32874331898720754 1108 26-27 0.6188942926653027 1108 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-0.4375305749689957 2316 56-57 -0.1269286907118996 2316 58-59 -0.2263144513669033 2316 60-61 0.15223709066273017 2316 62-63 -0.04387228031134072 2316 64-65 0.03203435202135552 2316 66-67 0.5155288860280898 2316 68-69 0.42646431115423056 2316 70-71 0.4130550464215723 2316 72-73 0.24845133774037365 2316 74-75 0.6211681881905093 2316 76-77 0.8722330234722762 2316 78-79 0.9595518986849854 2316 80-81 1.0413316623340911 2316 82-83 1.2805388284342563 2316 84-85 1.456497761987535 2316 86-87 1.299303144172196 2316 88-89 1.6275719766320371 2316 90-91 1.6433257682633275 2316 92-93 1.1543992643458516 2316 94-95 1.8976317069965312 2316 96-97 2.2985692884596993 2316 98-99 2.368970201739831 2316 100-101 2.5967756176762435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 2.0 5 28.0 6 71.0 7 178.0 8 344.0 9 661.0 10 926.0 11 1514.0 12 2098.0 13 2680.0 14 3388.0 15 4508.0 16 5604.0 17 6718.0 18 6551.0 19 6160.0 20 5858.0 21 5851.0 22 6301.0 23 6719.0 24 7623.0 25 8104.0 26 9003.0 27 10308.0 28 12661.0 29 14923.0 30 17735.0 31 21012.0 32 25413.0 33 31499.0 34 39266.0 35 51225.0 36 64221.0 37 67311.0 38 30238.0 39 1284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87797417932212 19.58957214496568 15.44470688656015 22.087746789152053 2 16.555352997934193 21.810293043436403 36.17907023958776 25.45528371904165 3 20.651521269317914 23.471042220636875 31.66516384268257 24.21227266736264 4 13.992133726647001 17.78258959392456 34.79215046339881 33.43312621602962 5 13.435567917162341 37.37496439522142 34.09618316773621 15.093284519880031 6 31.985187974643818 35.377727567522335 15.518734701562794 17.11834975627105 7 26.572731647105584 33.68878010000209 20.365488817757694 19.372999435134627 8 26.381514257620452 31.795016632147117 19.867570451264672 21.955898658967758 9 24.55490700642273 17.376514153015755 21.04458252264692 37.023996317914595 10-11 25.014958471934563 25.53222870771355 26.68267118558966 22.770141634762233 12-13 27.001297098265653 23.761375760999183 26.494905751166343 22.742421389568822 14-15 23.001736438000794 25.986945333584384 26.045000941442293 24.96631728697253 16-17 21.716981526810184 28.636477750580557 27.20579928450386 22.4407414381054 18-19 21.579321743132702 27.384464110127826 29.170903156969814 21.86531098976966 20-21 22.46019791209021 25.80158580723446 28.79610452101508 22.942111759660243 22-23 21.845436096989477 26.28297663131028 29.567564174982742 22.304023096717504 24-25 23.354794842961375 26.530087083867254 28.76539658463872 21.34972148853265 26-27 22.2993592258402 25.77481365241271 30.359513534719497 21.56631358702759 28-29 21.393229983890876 27.897863972049624 29.186384652398583 21.522521391660913 30-31 21.692399422582064 26.44846126488002 28.8192221594594 23.039917153078516 32-33 21.179021714912146 26.425162058999202 29.525648575962443 22.870167650126206 34-35 23.01204365511123 26.193171356354934 28.480231331916105 22.314553656617733 36-37 22.550525126574332 25.746893175446672 29.839951462404287 21.86263023557471 38-39 21.917038658173368 26.30237129547197 29.124868839648034 22.655721206706623 40-41 22.06508924639285 26.542629366210956 29.25072362646961 22.141557760926577 42-43 21.996627410449587 26.55422609321486 28.329856832318278 23.119289664017273 44-45 21.993376264179524 25.80798697848018 29.116821276293052 23.081815481047244 46-47 23.372769304797174 26.5166635285257 28.060942697545975 22.049624469131153 48-49 22.32912822443984 26.380468210632024 28.125483796732148 23.164919768195986 50-51 21.067386346994706 26.837695349275087 29.408251218644743 22.686667085085464 52-53 22.509466725245296 26.69260863197975 28.06564990899391 22.73227473378104 54-55 21.184378720012383 26.340145902939188 28.453374450039647 24.02210092700878 56-57 22.54095273959706 26.1754430008996 27.58488671311115 23.698717546392185 58-59 22.732065524383355 26.327119814222055 27.559781585388816 23.381033076005775 60-61 21.33789409820289 27.190317789075085 28.601435176468126 22.870352936253894 62-63 20.926379212954245 27.49325299692462 28.26785079185757 23.312516998263565 64-65 20.60241846063725 28.321094583568694 27.239377392832488 23.837109562961565 66-67 21.241619829765778 26.957238510968363 26.979205566661125 24.821936092604737 68-69 21.442551201759883 26.45974310989955 27.445231193768027 24.652474494572544 70-71 22.017720035984016 26.450030335362662 26.702650683068686 24.829598945584635 72-73 21.69909412330802 25.778572773488982 27.899956066026487 24.62237703717651 74-75 21.134019540157745 27.458628841607563 26.80641854432101 24.60093307391368 76-77 21.45505136090713 25.785790497709154 27.565639448524028 25.193518692859683 78-79 21.573254670599802 26.426285068725285 27.53436264356995 24.46609761710496 80-81 22.402874537124205 25.977007887194294 27.01510491851294 24.605012657168558 82-83 21.47856568270662 25.803505769550622 27.948872712133372 24.76905583560939 84-85 21.393190441469535 26.22915491970571 27.44839793246513 24.929256706359627 86-87 21.650883247329126 26.48434402658686 26.80642829571499 25.058344430369022 88-89 21.232579376743633 26.167520416243452 25.994190248948986 26.605709958063933 90-91 21.31260035670762 25.5117080646258 26.631728149043116 26.543963429623467 92-93 21.88168494298479 25.480008326594998 27.107250937003453 25.53105579341676 94-95 21.187158727170026 24.975731709868406 27.26814368501433 26.568965877947235 96-97 20.521245214335025 24.81987070859223 27.861043118056863 26.79784095901588 98-99 20.925751584761187 25.466641561538943 26.36770643737317 27.2399004163267 100-101 20.636649113888033 24.523679514296415 27.08828901204426 27.75138235977129 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1080.0 1 663.5 2 266.5 3 325.0 4 299.5 5 274.0 6 446.5 7 796.0 8 1050.0 9 1072.5 10 871.5 11 647.0 12 621.5 13 637.5 14 638.0 15 667.5 16 710.0 17 736.5 18 810.0 19 907.0 20 1015.5 21 1185.5 22 1383.5 23 1569.0 24 1782.0 25 2156.0 26 2687.0 27 3205.0 28 3621.5 29 4118.5 30 4617.5 31 5463.0 32 7157.5 33 7276.0 34 6210.5 35 6108.5 36 6431.0 37 7081.5 38 8254.0 39 9446.0 40 10493.5 41 11590.0 42 12458.5 43 12966.0 44 13574.5 45 15574.5 46 17420.0 47 21013.5 48 26860.0 49 26251.5 50 21858.0 51 20027.0 52 18714.0 53 17918.5 54 17602.5 55 16499.5 56 15134.5 57 14249.0 58 13389.5 59 11529.5 60 8969.0 61 7251.5 62 6178.0 63 4597.0 64 2963.5 65 2099.5 66 1601.5 67 1374.5 68 1101.0 69 784.0 70 572.5 71 395.5 72 303.5 73 272.5 74 195.0 75 149.5 76 113.0 77 73.0 78 51.5 79 26.0 80 15.5 81 9.5 82 8.5 83 5.0 84 2.5 85 2.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.0920939768614405E-4 2 0.044143182911776394 3 2.0920939768614405E-4 4 0.0 5 0.11004414318291178 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.230234942153601E-4 26-27 0.010983493378522564 28-29 0.0 30-31 0.0 32-33 1.0460469884307202E-4 34-35 0.012970982656540932 36-37 0.0020920939768614404 38-39 0.00930981819703341 40-41 0.003242745664135233 42-43 0.003974978556036737 44-45 0.0025105127722337287 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.0920939768614405E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.138140965292161E-4 68-69 7.322328919015042E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0013598610849599364 84-85 0.00617167723174125 86-87 0.0019874892780183686 88-89 1.0460469884307202E-4 90-91 0.0015690704826460803 92-93 7.322328919015042E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.276281930584322E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 477990.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.97673904720042 #Duplication Level Percentage of deduplicated Percentage of total 1 68.16405381541061 28.61304699408817 2 14.529152839633614 12.197729146523823 3 6.449471537925124 8.121833512194877 4 3.503915526332017 5.88331790769092 5 2.0920574648923678 4.390887513776728 6 1.3169560739162889 3.316891287084579 7 0.8977999325459045 2.63806994395515 8 0.6151588861303797 2.065789122852914 9 0.45195126744379227 1.7074296373985598 >10 1.801632426586655 12.39877296817177 >50 0.08967238446225365 2.69680742359256 >100 0.06924700800123468 6.121560461436242 >500 0.010461778187236896 3.122156857487893 >1k 0.008469058532525105 6.7257072237458555 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4500 0.9414422895876483 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3030 0.6339044749890165 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2730 0.5711416556831733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2441 0.5106801397518776 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2376 0.4970815289022783 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2057 0.4303437310403983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1837 0.38431766354944663 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1620 0.33891922425155335 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1543 0.32281010062972026 RNA PCR Primer, Index 27 (95% over 23bp) CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1363 0.28515240904621436 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1350 0.28243268687629447 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1272 0.26611435385677523 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1269 0.2654867256637168 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1237 0.2587920249377602 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1236 0.25858281554007406 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1218 0.2548170463817235 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1083 0.22657377769409404 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 925 0.19351869285968326 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 862 0.1803385008054562 RNA PCR Primer, Index 27 (96% over 25bp) CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 859 0.17971087261239777 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 841 0.17594510345404715 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 832 0.17406221887487186 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 797 0.1667398899558568 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 780 0.16318333019519235 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 778 0.16276491139982008 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 774 0.1619280738090755 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 753 0.15753467645766647 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 716 0.14979392874327915 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 709 0.14832946295947613 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 704 0.1472834159710454 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 676 0.14142555283583338 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 634 0.13263875813301534 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 604 0.12636247620243102 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 560 0.11715726270424068 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 555 0.11611121571580996 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 548 0.11464674993200694 No Hit GTACAAAGTCCTCAGCCACATTTGTAGAACTTTGGGGGATGTTTGCTCCA 514 0.10753363041067804 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 0.10648758342224733 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 494 0.10334944245695517 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 485 0.10146655787777988 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.0920939768614405E-4 0.0 20-21 0.0 0.0 0.0 9.414422895876483E-4 0.0 22-23 0.0 0.0 0.0 0.005021025544467457 0.0 24-25 0.0 0.0 0.0 0.01182033096926714 0.0 26-27 0.0 0.0 0.0 0.021234753865143622 0.0 28-29 0.0 0.0 0.0 0.05062867424004687 0.0 30-31 0.0 0.0 0.0 0.1163204251134961 0.0 32-33 0.0 0.0 0.0 0.23776648047030274 0.0 34-35 0.0 0.0 0.0 0.42479968200171553 0.0 36-37 0.0 0.0 0.0 0.6834871022406326 0.0 38-39 0.0 0.0 0.0 0.9816104939433878 0.0 40-41 0.0 0.0 0.0 1.264880018410427 0.0 42-43 0.0 0.0 0.0 1.548149542877466 0.0 44-45 0.0 0.0 0.0 1.8973200276156406 0.0 46-47 0.0 0.0 0.0 2.310613192744618 0.0 48-49 0.0 0.0 0.0 2.6729638695370195 0.0 50-51 0.0 0.0 0.0 3.0434737128391807 0.0 52-53 0.0 0.0 0.0 3.3812422854034603 0.0 54-55 0.0 0.0 0.0 3.783342747756229 0.0 56-57 0.0 0.0 0.0 4.27163748195569 0.0 58-59 0.0 0.0 0.0 4.737860624699262 0.0 60-61 0.0 0.0 0.0 5.222494194439214 0.0 62-63 0.0 0.0 0.0 5.679302914286909 0.0 64-65 0.0 0.0 0.0 6.1826607251197725 0.0 66-67 0.0 0.0 0.0 6.775350948764618 0.0 68-69 0.0 0.0 0.0 7.446808510638298 0.0 70-71 0.0 0.0 0.0 8.118893700705035 0.0 72-73 0.0 0.0 0.0 8.764513901964477 0.0 74-75 0.0 0.0 0.0 9.47561664469968 0.0 76-77 0.0 0.0 0.0 10.19174041297935 0.0 78-79 0.0 1.0460469884307202E-4 0.0 11.068536998681981 0.0 80-81 0.0 2.0920939768614405E-4 0.0 11.911860080754828 0.0 82-83 0.0 2.0920939768614405E-4 0.0 12.775162660306702 0.0 84-85 0.0 2.0920939768614405E-4 0.0 13.662628925291324 0.0 86-87 0.0 2.0920939768614405E-4 0.0 14.546643235214127 0.0 88-89 0.0 2.0920939768614405E-4 0.0 15.500010460469884 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATCCG 45 9.623073E-4 42.218967 9 TAAGGTA 125 1.3278623E-10 38.024914 4 CGGCGAA 25 0.0016162918 37.99707 62-63 AAGACCG 80 4.4725512E-4 29.706964 5 TAAGGTG 250 0.0 26.617441 5 GTAGTCA 125 7.3416013E-6 26.617441 4 GTGTAAG 145 7.7765617E-7 26.24331 1 AGGTAAG 240 7.2759576E-12 25.764917 2 GTAAGCG 75 1.0605108E-6 25.33138 94-95 TAGACAG 115 1.2745363E-4 24.798859 5 AAGGTAA 250 1.4551915E-11 24.734322 1 TGTAAGG 175 1.4625948E-7 24.462515 2 CTAGGCA 100 0.001645944 23.765574 4 GTAAGGT 320 0.0 22.280224 3 GGTAAGG 305 9.094947E-12 21.817574 3 GTACTTT 2405 0.0 21.625153 14-15 TTGGCGC 220 5.626498E-8 21.589245 9 ATAGGTT 110 0.0028762198 21.589245 8 CCCAGTA 155 3.8587234E-5 21.481422 2 GTTTAGG 135 3.756073E-4 21.140446 1 >>END_MODULE