##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938404_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478441 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.984608342512452 31.0 31.0 34.0 28.0 34.0 2 31.13386812585042 31.0 31.0 34.0 28.0 34.0 3 31.170181903306784 31.0 31.0 34.0 27.0 34.0 4 34.80702949789002 37.0 35.0 37.0 32.0 37.0 5 34.80109564188688 37.0 35.0 37.0 32.0 37.0 6 34.80968813291503 37.0 35.0 37.0 32.0 37.0 7 34.74953860559609 37.0 35.0 37.0 32.0 37.0 8 34.71697032653974 37.0 35.0 37.0 32.0 37.0 9 36.19761684303812 39.0 37.0 39.0 32.0 39.0 10-11 36.06705947023771 39.0 35.0 39.0 32.0 39.0 12-13 35.936661991760744 39.0 35.0 39.0 30.5 39.0 14-15 37.041115832464186 40.0 37.0 41.0 31.0 41.0 16-17 36.995287820232804 39.0 36.5 41.0 31.0 41.0 18-19 37.01286992544535 40.0 37.0 41.0 31.0 41.0 20-21 36.95122282580297 40.0 36.5 41.0 31.0 41.0 22-23 36.82152449309319 39.0 36.0 41.0 31.0 41.0 24-25 36.65794737491143 39.0 36.0 41.0 30.0 41.0 26-27 36.425915630140395 39.0 36.0 41.0 30.0 41.0 28-29 36.210406298791284 39.0 35.5 40.5 30.0 41.0 30-31 35.949128523684216 39.0 35.0 40.0 29.0 41.0 32-33 35.59692940195343 38.0 35.0 40.0 27.5 41.0 34-35 35.279641585900876 38.0 35.0 40.0 26.5 41.0 36-37 34.99914827533594 38.0 34.5 40.0 25.5 41.0 38-39 34.749863619547654 38.0 34.0 40.0 24.5 41.0 40-41 34.50997719677034 38.0 34.0 40.0 24.0 41.0 42-43 34.08062331614556 38.0 33.0 40.0 22.5 41.0 44-45 33.61368068372067 38.0 33.0 40.0 20.5 41.0 46-47 33.109538062164404 37.5 32.5 40.0 16.5 41.0 48-49 32.58665749799871 37.0 31.5 40.0 11.0 41.0 50-51 31.79278845249467 36.0 30.5 39.0 8.5 40.5 52-53 31.78820795040559 36.0 31.0 39.0 7.5 40.0 54-55 31.82773738036665 36.0 31.0 39.0 3.5 40.5 56-57 31.41373126467004 36.0 30.5 39.0 2.0 40.5 58-59 30.99232402741404 35.5 30.0 39.0 2.0 40.0 60-61 30.438313188041995 35.0 29.0 39.0 2.0 40.0 62-63 29.800684932938438 35.0 28.0 38.0 2.0 40.0 64-65 29.196260353941238 34.0 27.0 38.0 2.0 40.0 66-67 28.551289500690785 34.0 26.0 37.0 2.0 39.0 68-69 27.77417278201492 33.5 25.5 36.0 2.0 39.0 70-71 26.988232613843714 33.0 23.5 35.5 2.0 38.5 72-73 26.175371675922424 32.0 21.0 35.0 2.0 37.5 74-75 25.34987177102297 31.5 18.5 35.0 2.0 37.0 76-77 24.51693834767505 31.0 11.0 34.5 2.0 36.0 78-79 23.61503612775661 30.5 4.5 34.0 2.0 36.0 80-81 22.705555125919393 29.5 2.0 34.0 2.0 35.0 82-83 21.770036430824284 29.0 2.0 34.0 2.0 35.0 84-85 20.861340269751132 28.0 2.0 33.5 2.0 35.0 86-87 19.970376702665533 26.5 2.0 33.0 2.0 35.0 88-89 18.91859288815131 25.0 2.0 32.5 2.0 34.5 90-91 17.791204140113408 22.5 2.0 32.0 2.0 34.0 92-93 16.656705842517677 18.0 2.0 31.5 2.0 34.0 94-95 15.378812643565247 4.5 2.0 31.0 2.0 34.0 96-97 13.894390321899671 2.0 2.0 30.5 2.0 34.0 98-99 12.144207958766074 2.0 2.0 29.5 2.0 34.0 100-101 8.522640200150072 2.0 2.0 13.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8953678292534413 1101 2 0.576986318245762 1101 3 0.7714948639756898 1101 4 0.6132918891913093 1101 5 0.6699502140803872 1101 6 0.6042415794467431 1101 7 0.7338927060994891 1101 8 0.7039053504372674 1101 9 0.7717678127351917 1101 10-11 1.0202924877388284 1101 12-13 0.9334406977395773 1101 14-15 0.9350527145486964 1101 16-17 0.8781461553822325 1101 18-19 0.7696777728615487 1101 20-21 0.9865468785953766 1101 22-23 0.9940174613459973 1101 24-25 0.9230408418593399 1101 26-27 0.8293966178588832 1101 28-29 0.8907295298662063 1101 30-31 0.7725279112754819 1101 32-33 0.7114456600511474 1101 34-35 0.9329792599583513 1101 36-37 0.6958813060714348 1101 38-39 0.8016544718967964 1101 40-41 0.6103822333066944 1101 42-43 0.6941404846052563 1101 44-45 0.7862830428415464 1101 46-47 1.0096205244134566 1101 48-49 1.043822842887188 1101 50-51 0.5689118496469483 1101 52-53 0.7806595593520171 1101 54-55 0.7509040503745439 1101 56-57 1.2758393383534639 1101 58-59 0.8799259417236698 1101 60-61 0.8788089947012416 1101 62-63 0.6586092523841955 1101 64-65 0.5910734073343775 1101 66-67 0.7564789666811436 1101 68-69 0.784458667480731 1101 70-71 0.5733882646321646 1101 72-73 0.6038267903966847 1101 74-75 1.1143777545067834 1101 76-77 1.5142756020997936 1101 78-79 1.5416352363043018 1101 80-81 2.116735643723011 1101 82-83 2.1215053656125846 1101 84-85 2.441903416390222 1101 86-87 2.389811220591227 1101 88-89 2.4677309512270575 1101 90-91 3.044371349083697 1101 92-93 3.1965534046902917 1101 94-95 3.4454104203205205 1101 96-97 3.1086186184470552 1101 98-99 3.0644169630743807 1101 100-101 2.236416918658918 1102 1 0.6732535609066801 1102 2 0.553150102543956 1102 3 0.7173651299906467 1102 4 0.632902956290458 1102 5 0.6317345421671661 1102 6 0.7960722944833876 1102 7 0.8142153719290945 1102 8 0.7794348641964106 1102 9 0.7378320967767849 1102 10-11 0.8572505800443793 1102 12-13 0.8566306849345864 1102 14-15 0.611557300927565 1102 16-17 1.1213114294439137 1102 18-19 0.8871897183730724 1102 20-21 0.9336253976010624 1102 22-23 0.8957703001177109 1102 24-25 0.9804116833514911 1102 26-27 1.1180146255618055 1102 28-29 1.1495124080493966 1102 30-31 0.7466488760841798 1102 32-33 1.0056864038367408 1102 34-35 0.9505400338381662 1102 36-37 0.806203523477258 1102 38-39 0.891224632781288 1102 40-41 0.7585763310531206 1102 42-43 0.8686196901976047 1102 44-45 0.6961897208682828 1102 46-47 0.4072403905838655 1102 48-49 0.41014771097585623 1102 50-51 0.7381320406754917 1102 52-53 0.6119151957899334 1102 54-55 0.47820259598672266 1102 56-57 0.7530619010455979 1102 58-59 1.0951220274822333 1102 60-61 1.0781557907132608 1102 62-63 0.9469208371636952 1102 64-65 1.1471687322672395 1102 66-67 1.2879408780626385 1102 68-69 1.1214366897325192 1102 70-71 0.7258523533602883 1102 72-73 1.0815974266103936 1102 74-75 1.0259211793683924 1102 76-77 1.2939774501040588 1102 78-79 1.4527028336994547 1102 80-81 1.8866917480164211 1102 82-83 1.8796106256236484 1102 84-85 2.1258244340132784 1102 86-87 1.8445602776556456 1102 88-89 2.4595223046198775 1102 90-91 2.1465970258299336 1102 92-93 2.4755826170089925 1102 94-95 2.4459659675719294 1102 96-97 2.2061333541510546 1102 98-99 2.1674572763731366 1102 100-101 2.000645654651901 1103 1 0.5960964599269687 1103 2 0.4668805275998835 1103 3 0.5095766165292552 1103 4 0.3573735649458598 1103 5 0.6486528866861789 1103 6 0.6029215963425258 1103 7 0.4757424104232726 1103 8 0.5321107494081616 1103 9 0.7507968811631116 1103 10-11 0.6713657540010232 1103 12-13 0.7491854562066749 1103 14-15 0.682398348524579 1103 16-17 0.7397591267362102 1103 18-19 0.8139476062076838 1103 20-21 0.7078168463397816 1103 22-23 0.847732133798182 1103 24-25 0.8916060442247442 1103 26-27 1.0441770686691072 1103 28-29 0.9115924628206713 1103 30-31 0.48537158687643256 1103 32-33 0.8495014929364828 1103 34-35 0.8120256081650936 1103 36-37 0.8599864055261648 1103 38-39 1.0273355831785693 1103 40-41 1.2336134559470509 1103 42-43 1.0989001036821335 1103 44-45 1.14304567627984 1103 46-47 0.8861925042921186 1103 48-49 0.7704303423111938 1103 50-51 0.7869922582177189 1103 52-53 0.8053696430629458 1103 54-55 0.8774808301917716 1103 56-57 1.1704758870056367 1103 58-59 1.042221464340983 1103 60-61 1.3560500860152729 1103 62-63 1.380452812786622 1103 64-65 1.3134665607020182 1103 66-67 1.3734486694689458 1103 68-69 1.4817241424826548 1103 70-71 1.4448627097592706 1103 72-73 1.9158395966327646 1103 74-75 1.8089912634538692 1103 76-77 1.8295763662500129 1103 78-79 1.9622082529696883 1103 80-81 2.027235989318587 1103 82-83 2.3369194083128377 1103 84-85 2.5219783914305403 1103 86-87 2.707060759797127 1103 88-89 3.5760703644825647 1103 90-91 3.825165354920422 1103 92-93 3.785033072150547 1103 94-95 3.3654266441128584 1103 96-97 3.0522217403270933 1103 98-99 2.7213293545957686 1103 100-101 2.137113389744087 1104 1 0.23665157462903252 1104 2 0.20981985814381687 1104 3 0.10798413348207703 1104 4 0.1590886854190927 1104 5 0.1421152297824122 1104 6 0.2091562666642659 1104 7 -0.04249088924488831 1104 8 0.05109249807357941 1104 9 0.29573087337202963 1104 10-11 0.012460112679427482 1104 12-13 0.03876307848339877 1104 14-15 0.3021772404593648 1104 16-17 0.3089500804225409 1104 18-19 -0.012024475742968832 1104 20-21 0.0718200565547562 1104 22-23 0.044308205072169926 1104 24-25 -0.049969846700079756 1104 26-27 -0.022962413368112777 1104 28-29 -0.04975420432693056 1104 30-31 -0.19350772346839307 1104 32-33 -0.18315973920894635 1104 34-35 0.2016609421717348 1104 36-37 0.5137611133605304 1104 38-39 0.33816284475698666 1104 40-41 0.30803904388529446 1104 42-43 0.6059878255115976 1104 44-45 0.35833297248529306 1104 46-47 0.695973740765929 1104 48-49 0.7894616869944997 1104 50-51 0.9281154727610748 1104 52-53 0.8621796271922193 1104 54-55 1.1034447964304022 1104 56-57 1.400069380008091 1104 58-59 1.439458515271582 1104 60-61 1.5109925546345195 1104 62-63 1.5389616859650914 1104 64-65 1.4010486195679803 1104 66-67 1.56993005743605 1104 68-69 1.46987069420679 1104 70-71 1.7100075301315094 1104 72-73 1.9967757891013846 1104 74-75 2.111409745635374 1104 76-77 1.871205460175851 1104 78-79 1.96928244861245 1104 80-81 2.163345497326091 1104 82-83 1.934979753140425 1104 84-85 2.518610370942877 1104 86-87 2.633104079664392 1104 88-89 2.415334179227912 1104 90-91 2.261786152785085 1104 92-93 2.465323614640809 1104 94-95 2.7239652749020635 1104 96-97 2.3705605442514948 1104 98-99 1.8434843466604054 1104 100-101 1.1676960978670117 1105 1 0.4621492289969815 1105 2 0.5791280023473568 1105 3 0.2351469752892683 1105 4 0.0908244531381257 1105 5 0.17882965436294995 1105 6 0.26846060714015607 1105 7 0.21713042853542674 1105 8 0.27457417678538576 1105 9 0.28872356176221103 1105 10-11 0.5237952210856633 1105 12-13 0.7530038121801965 1105 14-15 0.5893079618997135 1105 16-17 0.6115154834063574 1105 18-19 0.7370710823484004 1105 20-21 0.6917706080952684 1105 22-23 0.8214499150590697 1105 24-25 0.8597486774535881 1105 26-27 0.6752920982755128 1105 28-29 0.7451804983483541 1105 30-31 0.7443710644103945 1105 32-33 0.8829828583143993 1105 34-35 0.7369341766081448 1105 36-37 0.5802798572851273 1105 38-39 0.747369978859517 1105 40-41 0.39304179102192904 1105 42-43 0.3621794488580292 1105 44-45 0.22416070588624848 1105 46-47 0.06228750603366251 1105 48-49 0.35556836042334794 1105 50-51 0.31385572042601595 1105 52-53 0.2611864316383503 1105 54-55 0.7491740555487851 1105 56-57 0.9701319301961675 1105 58-59 0.7477421888272247 1105 60-61 0.8475273587425463 1105 62-63 1.0947074279033053 1105 64-65 1.1522531333247983 1105 66-67 1.3020145001899515 1105 68-69 1.687547461883561 1105 70-71 1.3835230197557848 1105 72-73 1.6140087885519563 1105 74-75 1.4132101767245082 1105 76-77 1.5908115630455555 1105 78-79 1.8483302487899564 1105 80-81 1.8401321927320318 1105 82-83 2.233464601625272 1105 84-85 2.5734347655162253 1105 86-87 2.648851512148223 1105 88-89 2.6139796295470035 1105 90-91 2.818144717511853 1105 92-93 3.111302229238671 1105 94-95 3.33690217528666 1105 96-97 3.654984279512048 1105 98-99 3.30813926403499 1105 100-101 2.1535166464490665 1106 1 0.36848531715125077 1106 2 0.13165325487261015 1106 3 0.15874660797981122 1106 4 0.19302830988743835 1106 5 0.15949999412419658 1106 6 -0.01579099983872112 1106 7 0.3251653229339553 1106 8 -0.14181700778761552 1106 9 0.060945783984429625 1106 10-11 0.11201836158153355 1106 12-13 0.31638765056403884 1106 14-15 0.36231071672064985 1106 16-17 0.239385088548687 1106 18-19 0.5677414221096484 1106 20-21 0.5118119304093227 1106 22-23 0.3334563429745927 1106 24-25 0.4219387600982145 1106 26-27 0.394552887990848 1106 28-29 0.5093678261720456 1106 30-31 0.16083111950681683 1106 32-33 0.5963666966982402 1106 34-35 -0.061068578212790214 1106 36-37 0.3188243935569375 1106 38-39 0.2620623571882632 1106 40-41 0.35573692417160885 1106 42-43 -0.6676644446222255 1106 44-45 -0.01794214076205236 1106 46-47 0.17877051797122334 1106 48-49 0.16504953581361548 1106 50-51 -0.16812315928013888 1106 52-53 0.026016551013931632 1106 54-55 -0.27862667907012906 1106 56-57 -0.06418854730613077 1106 58-59 0.324923088735396 1106 60-61 0.9064116562632059 1106 62-63 0.9606394756535366 1106 64-65 0.6198839873192838 1106 66-67 0.013997971264331 1106 68-69 0.31077978511588356 1106 70-71 0.26522641736827524 1106 72-73 0.41299868754185454 1106 74-75 0.8255380004159676 1106 76-77 0.8562844739730124 1106 78-79 1.084349532536514 1106 80-81 0.5677033589395641 1106 82-83 1.0775417366115008 1106 84-85 1.3500187875547915 1106 86-87 1.0044300245449165 1106 88-89 0.6298428067738193 1106 90-91 1.2062301169608887 1106 92-93 1.3572847820394038 1106 94-95 0.6962456096300969 1106 96-97 1.0134553538922138 1106 98-99 1.9450309077448136 1106 100-101 1.2660051680284976 1107 1 0.3653921848715136 1107 2 0.471631989588186 1107 3 0.30808690086077206 1107 4 0.3588733632922967 1107 5 0.27158173658871476 1107 6 0.2963535718752297 1107 7 0.4650127606282837 1107 8 0.4556126315080462 1107 9 0.5542560799263043 1107 10-11 0.6443693837650954 1107 12-13 0.40944542125338756 1107 14-15 0.5467065087719405 1107 16-17 0.41179902053199413 1107 18-19 0.5418203292029133 1107 20-21 0.6093321888147472 1107 22-23 0.6096654032745548 1107 24-25 0.4695661504289603 1107 26-27 0.5664492841695434 1107 28-29 0.6974930980293763 1107 30-31 0.9219893525549949 1107 32-33 0.8609201257253503 1107 34-35 0.6187700794966773 1107 36-37 0.7766824800035437 1107 38-39 1.0985129878972586 1107 40-41 0.663465324603365 1107 42-43 0.6137300425138861 1107 44-45 0.6502106793046423 1107 46-47 0.7804693242154812 1107 48-49 0.4455559556741022 1107 50-51 0.6789983750423509 1107 52-53 0.7401515211297607 1107 54-55 0.687008540751691 1107 56-57 0.7705926780253307 1107 58-59 0.577460423808617 1107 60-61 0.49390339985542653 1107 62-63 0.9392936409105701 1107 64-65 0.7979203316235726 1107 66-67 1.3577827085897383 1107 68-69 1.3163619222769682 1107 70-71 1.5278788588484673 1107 72-73 1.0063001229485522 1107 74-75 1.1081065084629422 1107 76-77 1.2582225146670325 1107 78-79 1.3303079202413137 1107 80-81 1.4131252815145956 1107 82-83 1.5572994412495866 1107 84-85 1.7697665039532247 1107 86-87 1.8971591499295464 1107 88-89 2.1118176589639823 1107 90-91 2.2719813292432036 1107 92-93 2.497267141519373 1107 94-95 2.4572105219107954 1107 96-97 2.8379543309031536 1107 98-99 2.2995268238436015 1107 100-101 1.9585848725699257 1108 1 -0.13891048404701678 1108 2 -0.19778057259658155 1108 3 -0.23267069056376144 1108 4 -0.4632983068785421 1108 5 -0.3507331643943701 1108 6 -0.3200214630707947 1108 7 -0.4510325370735089 1108 8 -0.1857818391698487 1108 9 -0.3145230497358753 1108 10-11 -0.4337507692697571 1108 12-13 -0.30816584113091494 1108 14-15 -0.17384021932313232 1108 16-17 -0.236483167146325 1108 18-19 -0.35278821792346804 1108 20-21 -0.14670054746822103 1108 22-23 -0.23389131651006068 1108 24-25 -0.19256423556659286 1108 26-27 -0.2695571803655099 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1.751009716686827 1108 94-95 1.9641729180780345 1108 96-97 1.6809710628662184 1108 98-99 1.3241996716474578 1108 100-101 1.1582099448555283 1109 1 0.5502110553138415 1109 2 0.7075125186513382 1109 3 0.395217478983934 1109 4 0.31522957821439235 1109 5 0.356566756932267 1109 6 0.5276159186444644 1109 7 0.2862511661125424 1109 8 0.4125444838832095 1109 9 0.6237381370452368 1109 10-11 0.3415906247871092 1109 12-13 0.5445501480218411 1109 14-15 0.30059533224747526 1109 16-17 0.4210877157973769 1109 18-19 0.5583306579703944 1109 20-21 0.7367573886525207 1109 22-23 0.7235107922877333 1109 24-25 0.6426040770875119 1109 26-27 0.7242786076647079 1109 28-29 0.7725549136422316 1109 30-31 0.3764027910729979 1109 32-33 0.6891315939933378 1109 34-35 0.9561328750014795 1109 36-37 0.9236531879067869 1109 38-39 0.9616944314390068 1109 40-41 0.8044376285922681 1109 42-43 0.9133624195040895 1109 44-45 0.8572635463716409 1109 46-47 1.117531218324359 1109 48-49 1.3117137065015783 1109 50-51 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2316 68-69 -0.878569851602176 2316 70-71 -0.5539924861807748 2316 72-73 -0.27830026691499654 2316 74-75 -0.8577804311082069 2316 76-77 -0.34119269001132935 2316 78-79 -0.3997115729360772 2316 80-81 -0.470890313027315 2316 82-83 -1.1337336971519747 2316 84-85 -0.5362763810370268 2316 86-87 -0.377694651240045 2316 88-89 -0.39695427086057933 2316 90-91 -0.8693442068191786 2316 92-93 -0.9666704803129864 2316 94-95 -0.8693001993367631 2316 96-97 -0.5353400160754038 2316 98-99 -0.3690891605618578 2316 100-101 -0.2841051859096062 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5859.0 3 1107.0 4 927.0 5 776.0 6 842.0 7 1009.0 8 1188.0 9 1435.0 10 1896.0 11 2403.0 12 3236.0 13 3935.0 14 4628.0 15 5737.0 16 6733.0 17 7823.0 18 7437.0 19 7270.0 20 7735.0 21 8341.0 22 9345.0 23 10201.0 24 11449.0 25 12734.0 26 14461.0 27 16397.0 28 18575.0 29 20948.0 30 23757.0 31 26900.0 32 30834.0 33 35886.0 34 41005.0 35 45139.0 36 44123.0 37 29688.0 38 6599.0 39 83.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33200844459181 18.695411296110535 15.14948018752702 21.823100071770636 2 16.67513449206638 21.64271631112254 36.36063318425779 25.321516012553293 3 21.026538532309598 23.095130559876882 31.0180073936535 24.86032351416002 4 14.109258194364577 16.942025197344346 35.47806994615505 33.47064666213602 5 13.823301186121274 36.865552704067866 34.00137624369135 15.309769866119517 6 32.247931671978655 34.89681364552747 15.936164161250115 16.919090521243756 7 27.06659696811291 33.48207004704653 20.855828541557763 18.595504443282802 8 26.62435964453738 31.294720334553055 19.934971249346574 22.14594877156299 9 25.253108181008415 17.43905957921941 21.495690574464614 35.81214166530756 10-11 25.283147878834768 25.395121063818443 26.707538795938017 22.61419226140877 12-13 27.21947741580716 23.812769587498458 26.555008917426516 22.41274407926787 14-15 23.32761165633141 25.941045122496853 26.433311450183776 24.29803177098796 16-17 21.70714344700561 28.391169500720753 27.594630785489617 22.307056266784024 18-19 21.500060632163482 27.47107427649103 29.57427858179279 21.4545865095527 20-21 22.619623865286524 25.842978550347667 29.092992402660062 22.444405181705747 22-23 21.970511947961523 25.953897606225883 29.8083788889551 22.267211556857493 24-25 23.077365418715914 26.296777242463783 29.203887717889526 21.421969620930778 26-27 22.05232002576064 25.571407363110012 30.600795819750797 21.77547679137855 28-29 21.373589324077802 28.115457192291004 29.245566073734114 21.265387409897077 30-31 21.90518108388969 26.38037130508783 28.635111937255754 23.079335673766725 32-33 21.614122974923923 26.639165601226882 29.07526796289656 22.671443460952634 34-35 23.342306506160128 26.34265672125492 28.35770028905813 21.957336483526824 36-37 22.858280895693092 25.690279373529197 29.514611623435886 21.936828107341828 38-39 22.022682136510923 26.605414445489707 28.834117278143616 22.537786139855754 40-41 22.30570153983803 26.72798006530619 28.9437706172313 22.022547777624478 42-43 21.995752117669685 26.75434195452759 27.932753012400806 23.317152915401916 44-45 22.310667860961505 25.82990078085561 28.841070642818394 23.01836071536449 46-47 23.417481370605014 26.80040598440242 28.002471027619276 21.779641617373287 48-49 22.388092463193985 26.639768789752328 27.953235323323284 23.018903423730407 50-51 21.474418269215693 26.975501616397896 28.998272288574846 22.551807825811572 52-53 22.673745930381433 26.923080942949568 27.516004117957536 22.887169008711467 54-55 21.457984949832777 26.312395484949835 28.359113712374583 23.87050585284281 56-57 22.536168113530564 26.121833395695155 27.859219759569054 23.48277873120523 58-59 23.227056532903585 25.584187968533957 27.501329897025734 23.687425601536724 60-61 21.592854500113923 26.094013574443093 29.237623824464514 23.075508100978475 62-63 21.689937988433844 26.241772989379747 28.802506801622563 23.265782220563842 64-65 21.13664535251955 27.119203844489643 27.96535945936342 23.77879134362739 66-67 21.736504041419586 26.064804847613292 27.53746177529833 24.661229335668793 68-69 21.956593081565124 25.75736356168335 27.951852308618463 24.334191048133057 70-71 22.651383942896743 25.859418034269062 27.242681528509298 24.2465164943249 72-73 22.278175127017626 25.3733184079134 28.282416538368675 24.0660899267003 74-75 21.919202793650527 27.443163823856192 27.181337477959026 23.456295904534247 76-77 22.364012232262485 25.685242899873955 27.560006438032886 24.390738429830666 78-79 22.58703077379733 26.398010844617616 27.392693052170387 23.622265329414667 80-81 23.646410687614903 25.816313412509366 26.896509196035417 23.640766703840313 82-83 22.73576741210852 25.803137294250945 27.670191105703907 23.790904187936633 84-85 22.627677720363216 26.35882289001488 27.40440893660429 23.609090453017608 86-87 23.314270903876864 26.48339980101499 26.389968814534264 23.81236048057388 88-89 22.926387704246316 26.328855384409543 25.80214239124981 24.942614520094324 90-91 23.349731654334825 25.665532792112078 26.097373829450493 24.887361724102604 92-93 24.196710962274523 25.651316848427076 26.40480207033365 23.74717011896475 94-95 23.50351229302559 25.17530105368791 26.592448569994982 24.72873808329152 96-97 23.30639622943263 24.89638570336991 26.977347536723624 24.81987053047384 98-99 23.70342033638952 25.665169929803877 25.42477224932793 25.206637484478673 100-101 24.815006257141302 23.801202877866462 25.77868972531359 25.60510113967865 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 869.0 1 591.5 2 322.5 3 314.5 4 283.0 5 294.5 6 408.5 7 669.5 8 945.5 9 1060.5 10 877.5 11 658.0 12 591.0 13 605.5 14 656.0 15 664.0 16 723.5 17 772.5 18 838.5 19 960.0 20 1085.0 21 1291.5 22 1529.0 23 1723.5 24 1986.5 25 2404.5 26 2934.0 27 3470.0 28 3913.5 29 4350.0 30 4835.5 31 5775.5 32 7102.0 33 7113.5 34 6330.5 35 6212.0 36 6393.0 37 6828.5 38 7994.0 39 9217.0 40 10025.0 41 11115.5 42 12089.5 43 12502.5 44 13112.5 45 14772.0 46 17206.5 47 20950.0 48 25637.5 49 25968.0 50 22076.0 51 19162.0 52 17720.5 53 17419.5 54 17409.5 55 16639.5 56 15392.0 57 14317.5 58 13611.5 59 11962.0 60 9537.5 61 7762.5 62 6511.5 63 5059.0 64 3589.0 65 2608.0 66 2041.5 67 1689.5 68 1339.5 69 1012.0 70 762.0 71 561.5 72 425.5 73 339.5 74 266.0 75 204.0 76 150.0 77 112.5 78 82.0 79 50.5 80 28.0 81 20.0 82 13.5 83 10.0 84 5.5 85 3.0 86 2.5 87 1.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40193043656375604 2 0.033441950000104505 3 0.04033935218762606 4 0.04514663250014109 5 0.06855599750021424 6 0.05789637593768093 7 0.03992132781262475 8 0.03992132781262475 9 0.039085279062622144 10-11 0.042115955781381616 12-13 0.03427799875010712 14-15 0.0298887428125934 16-17 0.02539498078132936 18-19 0.030724791562596016 20-21 0.03793571203136855 22-23 0.03751768765636724 24-25 0.045355644687641736 26-27 0.04013034000012541 28-29 0.03521855359386006 30-31 0.038040218125118874 32-33 0.03271040734385222 34-35 0.03417349265635679 36-37 0.036472626718863976 38-39 0.019020109062559437 40-41 0.0165119628125516 42-43 0.01776603593755552 44-45 0.01160017640628625 46-47 0.020378688281313683 48-49 0.018497578593807806 50-51 0.013063261718790822 52-53 0.010137091093781678 54-55 0.00856949968752678 56-57 0.010450609375032659 58-59 0.00428474984376339 60-61 0.008778511875027433 62-63 0.013063261718790822 64-65 0.008778511875027433 66-67 0.004180243750013063 68-69 0.00794246312502482 70-71 0.016616468906301928 72-73 0.013063261718790822 74-75 0.014735359218796047 76-77 0.007106414375022208 78-79 0.0098235728125307 80-81 0.01160017640628625 82-83 0.014003816562543762 84-85 0.010241597187532005 86-87 0.002717158437508491 88-89 0.020274182187563355 90-91 0.029261706250091446 92-93 0.013376780000041801 94-95 0.02529047468757903 96-97 0.021841773593818257 98-99 0.014003816562543762 100-101 0.12269015406288342 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 478441.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.87758056908848 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48577175511576 32.79594253238793 2 13.394343715109075 12.290001659199607 3 5.742220805351812 7.903175929290715 4 3.053241259426865 5.603012875048847 5 1.8084357494040149 4.148332839865129 6 1.1242900028681575 3.0947823113762767 7 0.8001449672667212 2.569610064191077 8 0.5223231151410109 1.9170336638383207 9 0.38367970285839714 1.5842066832549035 >10 1.5324765513925733 11.447008438698388 >50 0.0799631815278757 2.58410358828958 >100 0.057573490698488945 5.591658888020292 >500 0.008681716851359446 2.7262324300331366 >1k 0.006853986987915352 5.744898096505815 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4483 0.9370016365654281 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2910 0.6082254656269006 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2663 0.5565994553142394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2422 0.506227518126582 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1988 0.4155162287512985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1625 0.3396448046885614 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1462 0.3055758181259549 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1390 0.2905269406259079 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1289 0.2694167096883419 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1266 0.2646094293758269 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1260 0.263355356250823 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1217 0.2543678321882949 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1160 0.24245413750075767 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1158 0.24203611312575637 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1112 0.2324215525007263 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 880 0.18393072500057478 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 823 0.17201703031303756 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 816 0.17055394500053297 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 809 0.1690908596880284 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 784 0.1638655550005121 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 778 0.16261148187550817 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 763 0.15947629906299837 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 720 0.15048877500047028 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 718 0.15007075062546896 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 687 0.14359137281294873 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.13773903156293044 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 652 0.13627594625042588 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 632 0.1320957025004128 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 585 0.12227212968788209 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 570 0.1191369468753723 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 563 0.11767386156286773 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 559 0.11683781281286512 No Hit GTACAAAGTCCTCAGCCACATTTGTAGAACTTTGGGGGATGTTTGCTCCA 504 0.10534214250032921 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 503 0.10513313031282855 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.0450609375032659E-4 0.0 20-21 0.0 0.0 0.0 9.405548437529393E-4 0.0 22-23 0.0 0.0 0.0 0.004180243750013063 0.0 24-25 0.0 0.0 0.0 0.010032585000031352 0.0 26-27 0.0 0.0 0.0 0.01985615781256205 0.0 28-29 0.0 0.0 0.0 0.04974490062515545 0.0 30-31 0.0 0.0 0.0 0.11913694687537231 0.0 32-33 0.0 0.0 0.0 0.24402172890701257 0.0 34-35 0.0 0.0 0.0 0.44091120953262786 0.0 36-37 0.0 0.0 0.0 0.696324102658426 0.0 38-39 0.0 0.0 0.0 0.9880006103155876 0.0 40-41 0.0 0.0 0.0 1.266927374535209 0.0 42-43 0.0 0.0 0.0 1.547212717973585 0.0 44-45 0.0 0.0 0.0 1.898875723443434 0.0 46-47 0.0 0.0 0.0 2.3113612754759734 0.0 48-49 0.0 0.0 0.0 2.6711757562583474 0.0 50-51 0.0 0.0 0.0 3.038096651415744 0.0 52-53 0.0 0.0 0.0 3.3674998589167737 0.0 54-55 0.0 0.0 0.0 3.767131161418022 0.0 56-57 0.0 0.0 0.0 4.265311710325829 0.0 58-59 0.0 0.0 0.0 4.725138522827266 0.0 60-61 0.0 0.0 0.0 5.197297054391242 0.0 62-63 0.0 0.0 0.0 5.644374123455139 0.0 64-65 0.0 0.0 0.0 6.1386879468941835 0.0 66-67 0.0 0.0 0.0 6.732491571583539 0.0 68-69 0.0 0.0 0.0 7.39526921814811 0.0 70-71 0.0 0.0 0.0 8.045924157837643 0.0 72-73 0.0 0.0 0.0 8.698773725495933 0.0 74-75 0.0 0.0 0.0 9.391126596591846 0.0 76-77 0.0 0.0 0.0 10.082538912844008 0.0 78-79 0.0 0.0 0.0 10.936144686596675 0.0 80-81 0.0 0.0 0.0 11.759652705349248 0.0 82-83 0.0 0.0 0.0 12.554421548320482 0.0 84-85 0.0 0.0 0.0 13.36946457347928 0.0 86-87 0.0 1.0450609375032659E-4 0.0 14.192136543481851 0.0 88-89 0.0 2.0901218750065317E-4 0.0 15.051594658484536 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTA 105 4.6215064E-8 36.213757 4 AGGTAAG 275 0.0 34.567677 2 TTGAACG 55 0.002570006 34.567677 4 AAGGTAA 265 0.0 34.200195 1 GTAAGCG 140 0.0 32.247818 94-95 TATGTAG 120 1.4942452E-7 31.68704 5 TAAGGTG 370 0.0 28.261412 5 ATAAGGG 70 0.008360481 27.160318 3 GGTAAGG 365 0.0 24.741934 3 AGTATCC 290 3.6379788E-12 22.940985 6 GAGTACT 2230 0.0 22.581186 12-13 GTAAGGT 410 0.0 22.026356 4 GGACTGA 175 4.025891E-6 21.723707 6 AAGGTGT 505 0.0 21.64305 6 GAATCCG 55 0.0028741413 21.59463 10-11 AGCGATC 55 0.0028828753 21.583342 68-69 GGTGTGC 515 0.0 21.218359 8 AGGTGTG 500 0.0 20.904694 7 GTACATG 1490 0.0 20.808844 1 CATGGGG 915 0.0 20.778385 4 >>END_MODULE