##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938404_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478441 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74420670469295 31.0 31.0 34.0 30.0 34.0 2 32.00421577582189 31.0 31.0 34.0 30.0 34.0 3 32.03905183711262 33.0 31.0 34.0 30.0 34.0 4 35.708035055524086 37.0 35.0 37.0 33.0 37.0 5 35.591485679529974 37.0 35.0 37.0 33.0 37.0 6 35.63777560869574 37.0 35.0 37.0 33.0 37.0 7 35.559715827029876 37.0 35.0 37.0 33.0 37.0 8 35.55062170675172 37.0 35.0 37.0 33.0 37.0 9 37.06029583585019 39.0 37.0 39.0 33.0 39.0 10-11 37.00374549840001 39.0 37.0 39.0 33.0 39.0 12-13 36.90649108249502 39.0 37.0 39.0 33.0 39.0 14-15 37.860373588383936 40.0 37.0 41.0 32.5 41.0 16-17 37.94092584038575 40.0 37.0 41.0 33.0 41.0 18-19 38.090468835237786 40.0 37.0 41.0 33.0 41.0 20-21 38.05027474652047 40.0 37.0 41.0 33.0 41.0 22-23 37.92943017007322 40.0 37.0 41.0 33.0 41.0 24-25 37.76114609742894 40.0 37.0 41.0 32.0 41.0 26-27 37.49729747241562 39.0 36.0 41.0 32.0 41.0 28-29 37.35413561964798 39.0 36.0 41.0 31.5 41.0 30-31 37.06773666972521 39.0 36.0 41.0 31.0 41.0 32-33 36.86734205471521 39.0 35.0 41.0 31.0 41.0 34-35 36.673270058377106 39.0 35.0 41.0 30.0 41.0 36-37 36.47047389333272 39.0 35.0 40.5 30.0 41.0 38-39 36.195119147397484 38.0 35.0 40.0 30.0 41.0 40-41 35.977525964539 38.0 35.0 40.0 29.5 41.0 42-43 35.664916886303644 38.0 35.0 40.0 28.5 41.0 44-45 35.32115767670413 38.0 34.0 40.0 27.0 41.0 46-47 34.83830190138387 38.0 34.0 40.0 25.5 41.0 48-49 34.3508896603761 38.0 33.0 40.0 24.0 41.0 50-51 33.88331267596214 37.5 33.0 40.0 23.0 41.0 52-53 33.282938753158696 37.0 32.5 40.0 21.0 41.0 54-55 32.84026348076357 36.5 31.5 39.5 18.5 41.0 56-57 32.351700627663604 36.0 31.0 39.0 16.0 40.0 58-59 31.751876406913286 35.5 30.5 39.0 10.5 40.0 60-61 31.160900717120818 35.0 30.0 38.5 7.5 40.0 62-63 30.68599890059589 35.0 29.0 38.0 3.5 40.0 64-65 30.03649457299855 34.5 28.5 37.5 2.0 40.0 66-67 29.32378600496195 34.0 27.5 37.0 2.0 39.0 68-69 28.60501921867064 34.0 26.0 36.0 2.0 39.0 70-71 27.814919707968173 33.0 25.5 35.5 2.0 38.5 72-73 27.31658762522443 33.0 25.0 35.0 2.0 37.5 74-75 26.698300312891245 32.0 24.0 35.0 2.0 37.0 76-77 25.587977409962775 30.5 22.5 34.0 2.0 35.5 78-79 26.121744373914446 32.0 23.5 34.5 2.0 36.0 80-81 25.805458771300955 32.0 22.5 35.0 2.0 36.0 82-83 25.22212665720538 31.0 20.0 34.0 2.0 35.0 84-85 24.58383269828464 31.0 18.0 34.0 2.0 35.0 86-87 23.869385148848032 30.5 10.5 34.0 2.0 35.0 88-89 22.98550604985777 30.0 4.5 34.0 2.0 35.0 90-91 22.24924598853359 29.0 2.0 34.0 2.0 35.0 92-93 21.326966125394772 29.0 2.0 34.0 2.0 35.0 94-95 20.29415434713998 27.5 2.0 33.0 2.0 35.0 96-97 19.016247562395364 25.5 2.0 33.0 2.0 34.0 98-99 17.32124964206663 21.0 2.0 32.0 2.0 34.0 100-101 13.034077347050106 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3477275259206678 1101 2 0.18730951346135782 1101 3 0.3867885295861342 1101 4 0.27498768208532454 1101 5 0.24460528985545693 1101 6 0.22572884415236416 1101 7 0.3080080967835741 1101 8 0.36158715636467775 1101 9 0.20439748735034868 1101 10-11 0.4482649676598811 1101 12-13 0.40875501525378866 1101 14-15 0.5514838204191079 1101 16-17 0.4236717388496132 1101 18-19 0.6013495213376885 1101 20-21 0.37821662532576283 1101 22-23 0.2065778146000028 1101 24-25 0.18472184699484728 1101 26-27 0.39750190340834735 1101 28-29 0.5055746579660081 1101 30-31 0.7423684819816998 1101 32-33 0.47720922818919576 1101 34-35 0.26172650081382187 1101 36-37 0.08381168775304104 1101 38-39 0.5788331055434739 1101 40-41 0.4462252377909195 1101 42-43 0.5730738034713028 1101 44-45 0.6419874938113495 1101 46-47 0.8133667425978572 1101 48-49 0.9545127208947122 1101 50-51 0.9785757229300245 1101 52-53 0.9578407094025323 1101 54-55 0.8806247965221345 1101 56-57 0.7467056615257803 1101 58-59 0.8023664830983783 1101 60-61 0.7245163830116432 1101 62-63 0.8829831639937815 1101 64-65 1.005886353540408 1101 66-67 0.8840229688561791 1101 68-69 1.1024059790582648 1101 70-71 1.2997850116656338 1101 72-73 1.3188887012108914 1101 74-75 1.3017070959573545 1101 76-77 1.3687360390654923 1101 78-79 1.3118310001321483 1101 80-81 1.1766004605935585 1101 82-83 1.8032163742402965 1101 84-85 1.7377965112253193 1101 86-87 1.853872203414948 1101 88-89 1.6309087114115535 1101 90-91 2.005230615200645 1101 92-93 2.4385203672775297 1101 94-95 2.501115082821137 1101 96-97 2.3814557908363057 1101 98-99 2.199866541555661 1101 100-101 1.9244029422877755 1102 1 0.2988991576351907 1102 2 0.23290923045612288 1102 3 0.364093303306511 1102 4 0.1474410086424811 1102 5 0.23716643742176302 1102 6 0.4041118279642646 1102 7 0.2134803617721417 1102 8 0.3790657192162499 1102 9 0.38005006852750967 1102 10-11 0.39996225170646227 1102 12-13 0.30052045971649477 1102 14-15 0.49173633283795937 1102 16-17 0.46556946833048585 1102 18-19 0.29571383078997115 1102 20-21 0.23247391096673198 1102 22-23 0.44095647361357493 1102 24-25 0.13640916606830444 1102 26-27 0.4104464034905604 1102 28-29 0.31364678444144545 1102 30-31 0.14973907434952594 1102 32-33 0.08614133184484274 1102 34-35 0.3117531571169181 1102 36-37 -0.018942132568533054 1102 38-39 0.08786884491956215 1102 40-41 0.34848130770516406 1102 42-43 0.06187068244172167 1102 44-45 0.20116697792469296 1102 46-47 0.4313893929817567 1102 48-49 0.3709315088550369 1102 50-51 0.3058378963903081 1102 52-53 0.1446889378794367 1102 54-55 0.47680422582812554 1102 56-57 0.3389414527197303 1102 58-59 0.8619894085155302 1102 60-61 0.8728076392459734 1102 62-63 1.0365807684142467 1102 64-65 1.0930848508224607 1102 66-67 1.1869282567691641 1102 68-69 1.7228949602892385 1102 70-71 1.6862092305363063 1102 72-73 1.7894397143995313 1102 74-75 1.7268028842614669 1102 76-77 1.6235553491904007 1102 78-79 1.6192578945980998 1102 80-81 1.7819401914396344 1102 82-83 1.7587576466677142 1102 84-85 1.6431292665902149 1102 86-87 1.901908356337426 1102 88-89 2.017412650067179 1102 90-91 2.518635995289632 1102 92-93 2.714674684851257 1102 94-95 2.18433855232583 1102 96-97 1.9735944235423446 1102 98-99 1.8452355694227087 1102 100-101 2.267648932631717 1103 1 0.18974714806950388 1103 2 0.2630141444420815 1103 3 0.31908539618623877 1103 4 0.19761747242401384 1103 5 0.17658536051055052 1103 6 0.18040782426142243 1103 7 0.2970258373564931 1103 8 0.017628359037281882 1103 9 0.45466628390975217 1103 10-11 0.4432754315259402 1103 12-13 0.36965231576833446 1103 14-15 0.5526766051514613 1103 16-17 0.5067274757361844 1103 18-19 0.23312463461623878 1103 20-21 0.3191701811202492 1103 22-23 0.49105097334357595 1103 24-25 0.5364853444220756 1103 26-27 0.49901820399970376 1103 28-29 0.5587819769123215 1103 30-31 0.6637910877723456 1103 32-33 0.5479099232203026 1103 34-35 0.6949929550032081 1103 36-37 0.7551094951410988 1103 38-39 0.46559535498668225 1103 40-41 0.24457981885228008 1103 42-43 0.32481618116887034 1103 44-45 0.44554520827260546 1103 46-47 0.5646147266236596 1103 48-49 0.48836188762537347 1103 50-51 0.7158678855903418 1103 52-53 0.9429512999869729 1103 54-55 1.1776257949093605 1103 56-57 1.5662735710565343 1103 58-59 0.8236254109840964 1103 60-61 0.948877511189206 1103 62-63 1.1767634036067172 1103 64-65 1.2508584178979838 1103 66-67 0.9605531892693548 1103 68-69 0.8250583291081846 1103 70-71 1.0417307147606358 1103 72-73 0.9664700121701095 1103 74-75 0.9458156513678446 1103 76-77 0.8207150537341015 1103 78-79 1.025411466302181 1103 80-81 1.3228257889093555 1103 82-83 1.5705609522296946 1103 84-85 1.7407532733787647 1103 86-87 1.9281368528274356 1103 88-89 1.5005111804999487 1103 90-91 1.476800579105106 1103 92-93 2.0068234929975226 1103 94-95 1.3405403381778704 1103 96-97 1.41347138103675 1103 98-99 1.717431916718862 1103 100-101 1.8490103073135042 1104 1 0.11651637328952802 1104 2 0.18824138437859972 1104 3 0.10770716793634705 1104 4 -0.12837264444224417 1104 5 -0.15557269748973823 1104 6 -0.01943707546445239 1104 7 -0.20311844196827877 1104 8 -0.11181724765727807 1104 9 -0.07905900825588219 1104 10-11 0.09643244350112923 1104 12-13 0.31570537449002245 1104 14-15 0.2805703073589356 1104 16-17 0.14948735886992637 1104 18-19 0.2707589947374416 1104 20-21 0.04690156908735332 1104 22-23 0.15453748322671146 1104 24-25 0.26503281231993014 1104 26-27 0.3788831224818736 1104 28-29 0.257102926270278 1104 30-31 0.24002016727025222 1104 32-33 0.3275367808166081 1104 34-35 0.4050365994989491 1104 36-37 0.5628888559836938 1104 38-39 0.4861235976645091 1104 40-41 0.5712823148845914 1104 42-43 0.5131638222019106 1104 44-45 0.6821091961531422 1104 46-47 0.5440909926747324 1104 48-49 0.6453738628093362 1104 50-51 0.8647686574847242 1104 52-53 0.7349185485802536 1104 54-55 0.8625558194368423 1104 56-57 0.9092029823968915 1104 58-59 1.0875213134512727 1104 60-61 0.8575712422525363 1104 62-63 0.8792323835518907 1104 64-65 1.0180682361060356 1104 66-67 0.8886660157013289 1104 68-69 0.929394824563385 1104 70-71 1.2603319267069644 1104 72-73 1.0158000150556958 1104 74-75 1.2656017572688683 1104 76-77 1.2220595566918284 1104 78-79 1.4303719698370223 1104 80-81 1.912459319424432 1104 82-83 1.966729434411203 1104 84-85 1.7609884486781446 1104 86-87 1.8301351214755393 1104 88-89 1.525521099703525 1104 90-91 1.7047348598726728 1104 92-93 1.8153107242772784 1104 94-95 2.146903958146126 1104 96-97 2.73826217601583 1104 98-99 2.4504745873291647 1104 100-101 1.749719079496451 1105 1 0.15985773671457437 1105 2 0.13718690654587817 1105 3 0.11472715863835248 1105 4 0.13217424706699177 1105 5 0.035471637069242945 1105 6 0.15713196219245162 1105 7 0.07132050820288782 1105 8 0.05714856105748112 1105 9 0.1420051902141708 1105 10-11 0.08281087213897109 1105 12-13 0.15473373339285956 1105 14-15 0.3388856786450205 1105 16-17 0.13023627058118592 1105 18-19 0.29779504562286263 1105 20-21 0.12545065830590119 1105 22-23 0.27698792910811676 1105 24-25 0.21442421589543414 1105 26-27 0.2993556299836726 1105 28-29 0.18175742100845582 1105 30-31 0.44706128979255055 1105 32-33 0.7692678995351727 1105 34-35 0.39339507969844334 1105 36-37 0.4563612366945691 1105 38-39 0.5608605065018324 1105 40-41 0.514720884682994 1105 42-43 0.555254181865493 1105 44-45 0.8320394611461666 1105 46-47 0.6812639777556626 1105 48-49 0.838954015803715 1105 50-51 0.8359850050609126 1105 52-53 0.9608980084509255 1105 54-55 0.9072496193607691 1105 56-57 0.8006931983640939 1105 58-59 0.9448244705452247 1105 60-61 0.8637438469607055 1105 62-63 0.3119993841013198 1105 64-65 0.5917696857489076 1105 66-67 0.7604486960614167 1105 68-69 0.9685732193903185 1105 70-71 1.0213458314447195 1105 72-73 1.0931614959736606 1105 74-75 1.3443135615036859 1105 76-77 1.0438929534206238 1105 78-79 1.2983847334564835 1105 80-81 1.2018574851824297 1105 82-83 1.5377217846922555 1105 84-85 1.6019862932324749 1105 86-87 1.7056708131200153 1105 88-89 1.7851572097580473 1105 90-91 1.6752897814736194 1105 92-93 1.7806566521755052 1105 94-95 1.745114092967139 1105 96-97 1.6344309769963452 1105 98-99 1.6755453789271506 1105 100-101 1.4063988478916993 1106 1 -0.1110329887216075 1106 2 -0.10753301999222842 1106 3 0.006692264239816836 1106 4 -0.21213245633062172 1106 5 0.11793261043931125 1106 6 -0.07881845103068486 1106 7 -0.0588447810533097 1106 8 -0.07792949220238654 1106 9 -0.060152752852857816 1106 10-11 -0.03810281013834782 1106 12-13 -0.2809871114188951 1106 14-15 -0.2736717134578299 1106 16-17 -0.23385922987795027 1106 18-19 -0.06634636851855902 1106 20-21 -0.1474602691504785 1106 22-23 -0.005564871626503987 1106 24-25 -0.041154296501261456 1106 26-27 -0.03278394761044723 1106 28-29 -0.10371089855077287 1106 30-31 0.0865700133921834 1106 32-33 0.26311546611000836 1106 34-35 -0.11861134821707964 1106 36-37 -0.1118664951695294 1106 38-39 -0.07701828137695799 1106 40-41 0.08641509955902649 1106 42-43 0.1771779651529073 1106 44-45 0.11578601762000318 1106 46-47 -0.24031545291467182 1106 48-49 0.24338468614347164 1106 50-51 0.6371328471178401 1106 52-53 0.5815591654757171 1106 54-55 0.5436132557244022 1106 56-57 0.6082459990987132 1106 58-59 0.8650724044295224 1106 60-61 1.0845197881911979 1106 62-63 0.8865632041196854 1106 64-65 1.0676420457121765 1106 66-67 1.1877902938575566 1106 68-69 1.1865484260018881 1106 70-71 0.5796332510957711 1106 72-73 0.7685058485907419 1106 74-75 0.6192446909802705 1106 76-77 0.8257111352388016 1106 78-79 0.6339448803802661 1106 80-81 0.5143689636030011 1106 82-83 1.1787915734893168 1106 84-85 1.5694794061801325 1106 86-87 1.3006432649106507 1106 88-89 1.3350883392346304 1106 90-91 1.4481777520888635 1106 92-93 1.2211295631029664 1106 94-95 1.0558349377788936 1106 96-97 1.3731821248384009 1106 98-99 1.4340623669895933 1106 100-101 1.5952877367805893 1107 1 0.1380608201808151 1107 2 0.11273182945922855 1107 3 -0.09151065812578452 1107 4 -0.03996823033864416 1107 5 -0.0996338032993549 1107 6 -0.09900838868473727 1107 7 -0.03287938547068592 1107 8 0.12179502570394618 1107 9 0.17411576969317366 1107 10-11 0.23661204172961448 1107 12-13 0.15960702773983826 1107 14-15 0.07317594977739361 1107 16-17 0.044909316833177115 1107 18-19 -0.02279683812691502 1107 20-21 0.10960802141062942 1107 22-23 0.10706058549656206 1107 24-25 -0.06076628561085329 1107 26-27 0.17594609604783074 1107 28-29 0.19433938095883718 1107 30-31 0.13022627295752898 1107 32-33 -0.03578260551533674 1107 34-35 0.04909240381681457 1107 36-37 0.19451470116382552 1107 38-39 0.054959390074401426 1107 40-41 0.28370901156586115 1107 42-43 0.5150805153760345 1107 44-45 0.30085037732551 1107 46-47 -0.03106988720977455 1107 48-49 -0.05222089116066542 1107 50-51 0.5231017869145305 1107 52-53 0.4613941984207983 1107 54-55 0.1588888183683892 1107 56-57 0.1592967780203054 1107 58-59 0.3192352449560012 1107 60-61 0.34019254446203817 1107 62-63 0.6440745214396131 1107 64-65 0.6057161681164409 1107 66-67 0.2110069097775238 1107 68-69 -0.07590666719483252 1107 70-71 -0.01778229189392988 1107 72-73 0.3758834523798278 1107 74-75 0.4136561097936031 1107 76-77 0.6066787056800571 1107 78-79 0.7924658959586957 1107 80-81 0.5162115521680768 1107 82-83 0.6452000763810695 1107 84-85 0.9193175000373657 1107 86-87 1.4569733117909358 1107 88-89 1.5518593185654197 1107 90-91 0.9852490230118072 1107 92-93 1.1205645026096747 1107 94-95 1.1174337810763042 1107 96-97 0.2606936032589715 1107 98-99 0.3716821855902701 1107 100-101 0.5690605526586676 1108 1 -0.10062528604742838 1108 2 -0.11424688123368298 1108 3 0.008287516638787906 1108 4 -0.0326726501964103 1108 5 -0.08986423640022423 1108 6 -0.09473444252267171 1108 7 0.097039386574032 1108 8 -0.010490501473526592 1108 9 -0.008767765273908878 1108 10-11 -0.14163793816445747 1108 12-13 -0.08353302059099121 1108 14-15 -0.009982174741494987 1108 16-17 -0.19882050543041174 1108 18-19 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1023.0 11 1642.0 12 2310.0 13 2844.0 14 3729.0 15 4866.0 16 5931.0 17 7478.0 18 7340.0 19 6654.0 20 6877.0 21 7052.0 22 7597.0 23 8019.0 24 8999.0 25 10008.0 26 10761.0 27 12471.0 28 14787.0 29 17449.0 30 20206.0 31 24051.0 32 28944.0 33 34722.0 34 43441.0 35 53779.0 36 60810.0 37 49815.0 38 13324.0 39 218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76521684593433 19.61470517524329 15.453813749777876 22.1662642290445 2 16.62879635107141 21.92011906678066 36.02188186551892 25.429202716629007 3 20.656897232829813 23.54508800642099 31.548958993041836 24.24905576770736 4 14.08951155941903 17.79843282661812 34.72883803854603 33.38321757541682 5 13.593972075913385 37.2937045397542 34.119220800936375 14.993102583396038 6 31.811445925411913 35.34479695511045 15.576215249111176 17.26754187036646 7 26.609550602895656 33.726624599480395 20.314312527563484 19.34951227006047 8 26.492141125323972 31.787057938299473 19.841359418108855 21.879441518267704 9 24.465712595701454 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29.568377126840755 22.80355782404635 34-35 23.023040704024414 26.268910988362066 28.397079803717656 22.310968503895857 36-37 22.687664338236832 25.62171584795395 29.819578040022236 21.87104177378698 38-39 21.924957068603483 26.234924364442875 29.105866785679567 22.73425178127407 40-41 22.12323154620556 26.524507508612327 29.208439245459715 22.143821699722398 42-43 22.029363566435904 26.53537925153005 28.36694424935621 23.068312932677838 44-45 22.117204441541478 25.64880470280862 29.07736120182887 23.15662965382103 46-47 23.42995270054197 26.481112613676505 28.010559295712532 22.078375390068995 48-49 22.32939902725728 26.328220198519777 28.059572653681435 23.28280812054151 50-51 21.08609002990964 26.75732221945862 29.40874632399815 22.747841426633585 52-53 22.491278966476536 26.746453585708586 27.936359969149798 22.82590747866508 54-55 21.295102217410296 26.289030413363403 28.34602385665108 24.06984351257522 56-57 22.48354551553901 26.357899929144867 27.44288219446076 23.71567236085536 58-59 22.765502956477395 26.256529018207054 27.52115725867975 23.4568107666358 60-61 21.360836550379254 27.17430153352242 28.593285274464353 22.871576641633972 62-63 21.038748769440748 27.454586876960796 28.212674081025664 23.293990272572792 64-65 20.680397373970877 28.176828490869305 27.258637951178933 23.88413618398089 66-67 21.294475180847794 26.835388271490114 26.938849304302938 24.93128724335916 68-69 21.454168090569635 26.371205494541094 27.405403202292455 24.769223212596813 70-71 22.067297744131462 26.39740323258249 26.6084010358644 24.926897987421647 72-73 21.640644594933534 25.70343198729203 27.86608561157094 24.789837806203494 74-75 21.130296107566032 27.4388064568045 26.67518042977086 24.755717005858614 76-77 21.374213330379295 25.773919877268042 27.560242537742376 25.291624254610284 78-79 21.603081675692508 26.36500634351989 27.422608012273198 24.609303968514403 80-81 22.409056089524192 25.836337015783574 26.95016412699176 24.804442767700476 82-83 21.512513180982598 25.820721915493312 27.80301628955209 24.863748613971996 84-85 21.391678772613734 26.2413385867918 27.3719469498239 24.995035690770564 86-87 21.751149087731985 26.475524797093787 26.59090386540781 25.182422249766418 88-89 21.202614324441257 26.280565419769626 25.8074663333619 26.709353922427216 90-91 21.348772560535913 25.536384252824313 26.4626333775748 26.65220980906497 92-93 21.848948508641115 25.59769835057515 26.83881661108667 25.714536529697067 94-95 21.233548128191355 24.869315129765216 27.122884535397258 26.77425220664617 96-97 20.55969283568925 24.72655980570227 27.76622404852427 26.947523310084208 98-99 20.866209208658955 25.46562690070458 26.228939409456963 27.439224481179497 100-101 20.85136934334641 24.332049301794786 26.746244573521082 28.07033678133772 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 986.0 1 619.0 2 265.5 3 302.0 4 268.5 5 263.0 6 420.5 7 719.0 8 971.5 9 1060.5 10 883.5 11 624.5 12 580.5 13 595.0 14 617.5 15 663.0 16 690.0 17 726.5 18 817.0 19 866.5 20 952.5 21 1134.5 22 1341.5 23 1501.5 24 1752.5 25 2208.0 26 2627.0 27 3160.5 28 3702.0 29 4085.0 30 4623.0 31 5563.5 32 7074.5 33 7168.0 34 6301.0 35 6274.0 36 6449.5 37 7032.0 38 8176.0 39 9285.5 40 10293.0 41 11421.0 42 12428.0 43 12987.5 44 13667.0 45 15551.5 46 17534.5 47 21263.0 48 26641.5 49 26102.0 50 21968.5 51 20168.5 52 18823.0 53 17919.0 54 17598.0 55 16712.5 56 15475.0 57 14381.0 58 13292.5 59 11548.0 60 9151.5 61 7363.5 62 6303.0 63 4649.0 64 2997.5 65 2226.5 66 1696.5 67 1348.0 68 1075.0 69 806.5 70 597.0 71 417.5 72 305.5 73 257.0 74 187.5 75 127.0 76 100.5 77 80.5 78 56.0 79 25.5 80 14.0 81 9.5 82 6.5 83 3.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022155291875069236 2 0.011286658125035272 3 0.0020901218750065315 4 0.0 5 2.0901218750065314E-4 6 0.0 7 0.0 8 2.0901218750065314E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018811096875058783 26-27 0.009092030156278411 28-29 2.0901218750065314E-4 30-31 0.0 32-33 5.225304687516329E-4 34-35 0.010137091093781678 36-37 0.0014630853125045722 38-39 0.012854249531290168 40-41 0.011913694687537231 42-43 0.0052253046875163295 44-45 7.315426562522861E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.225304687516329E-4 70-71 0.0 72-73 2.0901218750065314E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0450609375032657E-4 82-83 0.0011495670312535924 84-85 0.005434316875016982 86-87 0.0037622193750117567 88-89 0.0 90-91 0.001254073125003919 92-93 3.1351828125097974E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 478441.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.943112511225365 #Duplication Level Percentage of deduplicated Percentage of total 1 67.82327037770185 28.447190603312073 2 14.924103926574306 12.519267402430529 3 6.518786468694929 8.202545828793747 4 3.41283195190278 5.725791781622528 5 2.0384580237030403 4.274963711879336 6 1.340442874647196 3.373340778371862 7 0.8803081607242916 2.584600496086618 8 0.6168256603078968 2.069727045608399 9 0.4351084344907253 1.642482182018486 >10 1.8346550018391619 12.64568042768634 >50 0.08585746022567033 2.5450770646432885 >100 0.06988397925328628 6.038381673350369 >500 0.010981768168373558 3.2592312162908774 >1k 0.008485911766470478 6.671719787905511 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4400 0.919653625002874 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3067 0.6410403790645033 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2622 0.5480299556267126 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2497 0.5219034321891309 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2416 0.504973445001578 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2083 0.4353723865638605 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1720 0.35950096250112346 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1555 0.32501395156351565 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1487 0.3108011228134712 RNA PCR Primer, Index 27 (95% over 23bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1368 0.28592867250089354 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1311 0.2740149778133563 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1305 0.27276090468835235 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1298 0.2712978193758478 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1269 0.2652364659383289 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1255 0.26231029531331973 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1107 0.23137649156322304 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1106 0.2311674793757224 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 954 0.1993976268756231 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 906 0.18936504187559178 RNA PCR Primer, Index 27 (96% over 25bp) GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 887 0.18539381031307936 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 856 0.1789144325005591 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 825 0.17243505468803885 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 820 0.1713899937505356 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 810 0.16929987187552906 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 795 0.16616468906301926 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 787 0.16449259156301405 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 772 0.16135740875050425 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 720 0.15048877500047028 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 709 0.1481896409379631 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 682 0.14254631187544545 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 621 0.1297965684379056 No Hit GTACAAAGTCCTCAGCCACATTTGTAGAACTTTGGGGGATGTTTGCTCCA 590 0.12331719062538536 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 585 0.12227212968788209 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 584 0.12206311750038144 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.11307559343785335 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 540 0.11286658125035272 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 539 0.11265756906285206 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 535 0.11182152031284943 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 509 0.10638720343783246 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 497 0.10387905718782463 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 480 0.10032585000031352 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.0450609375032659E-4 0.0 20-21 0.0 0.0 0.0 0.0010450609375032658 0.0 22-23 0.0 0.0 0.0 0.004389255937513716 0.0 24-25 0.0 0.0 0.0 0.009823572812530698 0.0 26-27 0.0 0.0 0.0 0.019751651718811724 0.0 28-29 0.0 0.0 0.0 0.049326876250154146 0.0 30-31 0.0 0.0 0.0 0.11850991031287035 0.0 32-33 0.0 0.0 0.0 0.2437082106257616 0.0 34-35 0.0 0.0 0.0 0.4397616425013743 0.0 36-37 0.0 0.0 0.0 0.6946520051584208 0.0 38-39 0.0 0.0 0.0 0.989777213909343 0.0 40-41 0.0 0.0 0.0 1.270062557347719 0.0 42-43 0.0 0.0 0.0 1.5510794434423472 0.0 44-45 0.0 0.0 0.0 1.901174857505941 0.0 46-47 0.0 0.0 0.0 2.31366040953848 0.0 48-49 0.0 0.0 0.0 2.6739974207896067 0.0 50-51 0.0 0.0 0.0 3.040186773290751 0.0 52-53 0.0 0.0 0.0 3.3690674503230285 0.0 54-55 0.0 0.0 0.0 3.7681762223555255 0.0 56-57 0.0 0.0 0.0 4.26249004579457 0.0 58-59 0.0 0.0 0.0 4.720331242514751 0.0 60-61 0.0 0.0 0.0 5.191026688766222 0.0 62-63 0.0 0.0 0.0 5.635491105486361 0.0 64-65 0.0 0.0 0.0 6.127610300956649 0.0 66-67 0.0 0.0 0.0 6.721936456114756 0.0 68-69 0.0 0.0 0.0 7.38878984033559 0.0 70-71 0.0 0.0 0.0 8.042893481118885 0.0 72-73 0.0 0.0 0.0 8.705253103308454 0.0 74-75 0.0 1.0450609375032659E-4 0.0 9.416208059091925 0.0 76-77 0.0 2.0901218750065317E-4 0.0 10.126954002687896 0.0 78-79 0.0 2.0901218750065317E-4 0.0 11.003969141440638 0.0 80-81 0.0 2.0901218750065317E-4 0.0 11.85308115316204 0.0 82-83 0.0 2.0901218750065317E-4 0.0 12.682650525352134 0.0 84-85 0.0 2.0901218750065317E-4 0.0 13.538032902698557 0.0 86-87 0.0 2.0901218750065317E-4 0.0 14.40585150520127 0.0 88-89 0.0 2.0901218750065317E-4 0.0 15.324146550985388 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGCG 45 9.617454E-4 42.223984 2 AGGTAAG 175 0.0 35.28719 2 GGTAAGG 195 0.0 34.100426 3 GTAAGGT 220 0.0 30.225378 4 GTACTAG 80 4.4859163E-4 29.691843 1 ACTTAGT 65 0.0058424976 29.228937 8 ACCGTGC 85 6.404018E-4 27.939425 8 AGTACCG 70 0.008389327 27.141153 5 TAAGGTG 240 9.094947E-12 25.727552 5 GGCTTAC 130 9.99377E-6 25.580667 1 CATAGAC 130 1.0009997E-5 25.57532 3 GTAAGCG 75 1.0603799E-6 25.331745 94-95 GTAGTAC 120 1.7095098E-4 23.74851 3 TAACTCA 120 1.7095098E-4 23.74851 4 AAGGTAA 235 4.623871E-9 22.237295 1 GAGTTCG 110 0.002875984 21.589556 7 CTCCGAC 280 0.0 21.204027 94-95 GTGTAAG 115 0.0037169945 20.655195 1 GAGTACT 2435 0.0 19.603493 12-13 CCTTATA 125 0.0060210163 19.002783 1 >>END_MODULE