FastQCFastQC Report
Thu 26 May 2016
SRR938403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691368
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54570.2027593402314362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.15482832522345513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30280.11250783987919898No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37800.051.3365551
GTATCAA67550.040.9784971
ATCAACG82950.033.0864453
TCAACGC83800.032.7508434
TATCAAC85550.032.3588942
CAACGCA86000.031.9683425
AACGCAG89700.030.7841326
ACGCAGA102850.026.4308267
CGCAGAG104200.026.087428
GCAGAGT113150.022.8453949
GTACATG74850.022.6847921
TACATGG72000.022.1971652
AGAGTAC100050.020.82624210-11
GAGTACT69650.020.73759312-13
CAGAGTA107150.020.24449710-11
GTGTAAG12250.020.1895031
ACATGGG78100.019.8544223
CCGTATA1707.8752724E-519.5857332
GTACTTT73550.019.57046914-15
TAAGGTG15800.019.2669775
Produced by FastQC (version 0.11.5)