##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938403_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2691368 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.106643164368457 31.0 31.0 34.0 28.0 34.0 2 31.15455337211411 31.0 31.0 34.0 27.0 34.0 3 31.205039964806 31.0 31.0 34.0 27.0 34.0 4 34.84949215417587 37.0 35.0 37.0 32.0 37.0 5 34.83057389401969 37.0 35.0 37.0 32.0 37.0 6 34.84030537629934 37.0 35.0 37.0 32.0 37.0 7 34.74521878836339 37.0 35.0 37.0 31.0 37.0 8 34.713559795613236 37.0 35.0 37.0 31.0 37.0 9 36.21092507602082 39.0 35.0 39.0 32.0 39.0 10-11 36.05112492977549 39.0 35.0 39.0 30.0 39.0 12-13 35.9166864211806 38.0 35.0 39.0 30.0 39.0 14-15 36.89479160783661 39.0 36.0 41.0 30.5 41.0 16-17 36.71607728857592 39.0 36.0 41.0 30.0 41.0 18-19 36.8536504483965 39.0 36.0 41.0 30.0 41.0 20-21 36.83040632124629 39.0 36.0 41.0 30.0 41.0 22-23 36.74161727418918 39.0 36.0 41.0 30.0 41.0 24-25 36.61149199960764 39.0 36.0 41.0 30.0 41.0 26-27 36.40236229307921 39.0 36.0 41.0 29.5 41.0 28-29 36.19747150891294 39.0 35.0 40.5 29.0 41.0 30-31 35.9521234182765 39.0 35.0 40.0 27.5 41.0 32-33 35.693525002898156 38.0 35.0 40.0 27.0 41.0 34-35 35.45186146970612 38.0 34.0 40.0 26.5 41.0 36-37 35.1787862157832 38.0 34.0 40.0 25.5 41.0 38-39 35.04821860109803 38.0 34.0 40.0 25.0 41.0 40-41 34.956574500402766 38.0 34.0 40.0 25.0 41.0 42-43 34.686566459882116 38.0 33.5 40.0 24.0 41.0 44-45 34.358825511784346 38.0 33.0 40.0 24.0 41.0 46-47 34.01035254190434 38.0 33.0 40.0 22.5 41.0 48-49 33.668220956777375 38.0 33.0 40.0 21.0 41.0 50-51 32.981007613971784 37.0 31.5 39.5 19.0 40.5 52-53 33.00471563160445 37.0 32.0 39.5 19.0 40.0 54-55 33.1166512717696 37.0 32.0 40.0 18.5 41.0 56-57 32.79675633358203 37.0 31.5 40.0 15.5 41.0 58-59 32.42859597795619 36.5 31.0 39.5 13.0 41.0 60-61 31.95107971113575 36.0 31.0 39.0 8.5 40.5 62-63 31.382969924588537 35.5 30.0 39.0 7.5 40.0 64-65 30.870574183835135 35.0 29.0 38.5 4.5 40.0 66-67 30.329254119094827 35.0 28.5 38.0 2.0 40.0 68-69 29.74518256143344 34.0 28.0 37.5 2.0 40.0 70-71 29.106690352266952 34.0 26.5 37.0 2.0 39.0 72-73 28.464521016821184 34.0 26.0 36.0 2.0 39.0 74-75 27.78882375059821 33.0 26.0 36.0 2.0 38.5 76-77 27.032163940419892 32.5 24.5 35.0 2.0 37.5 78-79 26.256359219549317 32.0 22.0 35.0 2.0 37.0 80-81 25.56190011919589 31.5 20.0 35.0 2.0 36.5 82-83 24.824448198834197 31.0 17.5 34.5 2.0 36.0 84-85 24.10774632083015 31.0 10.5 34.0 2.0 35.5 86-87 23.33435282726108 30.0 4.5 34.0 2.0 35.0 88-89 22.509125842322565 29.5 2.0 34.0 2.0 35.0 90-91 21.585815652114462 29.0 2.0 34.0 2.0 35.0 92-93 20.639880165031315 28.0 2.0 34.0 2.0 35.0 94-95 19.618520952913165 26.5 2.0 33.0 2.0 35.0 96-97 18.364390154003466 24.5 2.0 33.0 2.0 34.5 98-99 16.839050066731865 11.0 2.0 32.0 2.0 34.0 100-101 13.326461858801917 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5727132067585892 1101 2 0.4447786744576234 1101 3 0.4271342455726135 1101 4 0.33960166526718893 1101 5 0.3262087250424912 1101 6 0.5313313188158872 1101 7 0.6196795091031362 1101 8 0.6262765173031823 1101 9 0.8096452545267496 1101 10-11 0.8132741371052745 1101 12-13 0.8879485478510318 1101 14-15 1.1102759262851194 1101 16-17 0.8476110389724667 1101 18-19 0.943605685508011 1101 20-21 0.9016474990586403 1101 22-23 0.9592311693567055 1101 24-25 0.8861766540436378 1101 26-27 0.9961051300357013 1101 28-29 0.9007980551699504 1101 30-31 0.9401678255129724 1101 32-33 0.9341641106126701 1101 34-35 0.9652784101729708 1101 36-37 1.0010250190000463 1101 38-39 1.0247991378380092 1101 40-41 0.9850833050941006 1101 42-43 1.257624589182555 1101 44-45 1.1214013479579066 1101 46-47 1.063262826482351 1101 48-49 1.0882179145591522 1101 50-51 1.1810358759879662 1101 52-53 1.1322117519722639 1101 54-55 1.247685679028912 1101 56-57 1.3014742987447647 1101 58-59 1.198763894954844 1101 60-61 1.2998501625751757 1101 62-63 1.1042462387684289 1101 64-65 1.056199108614397 1101 66-67 1.1402522301661797 1101 68-69 1.2461938524860834 1101 70-71 1.204309801331494 1101 72-73 1.199731458958965 1101 74-75 1.2198969655888163 1101 76-77 1.4176516783516888 1101 78-79 1.6304009446612575 1101 80-81 1.6524494525005693 1101 82-83 1.59823157431223 1101 84-85 1.553709710284064 1101 86-87 1.6456273289550367 1101 88-89 1.7930391360155298 1101 90-91 1.6707629262706547 1101 92-93 1.4908966890155924 1101 94-95 1.582496682717757 1101 96-97 1.4203418861369173 1101 98-99 1.415468381046022 1101 100-101 1.3328751415789686 1102 1 0.4040138725658373 1102 2 0.2848251518110061 1102 3 0.3105493261792276 1102 4 0.22800622645723934 1102 5 0.29016689844206667 1102 6 0.3739489576383903 1102 7 0.36512773401948806 1102 8 0.40984495325966463 1102 9 0.5566858764940434 1102 10-11 0.592707304711027 1102 12-13 0.515594244486266 1102 14-15 0.69147150354339 1102 16-17 0.8646399103350291 1102 18-19 0.6996627239210795 1102 20-21 0.665250694957578 1102 22-23 0.6934285825337909 1102 24-25 0.670758632466466 1102 26-27 0.6547294673667778 1102 28-29 0.5417277591536873 1102 30-31 0.6017040133922933 1102 32-33 0.6642408908866244 1102 34-35 0.41238529903289134 1102 36-37 0.6283943018208689 1102 38-39 0.339595922384909 1102 40-41 0.4150513304131209 1102 42-43 0.5734742961114918 1102 44-45 0.8704918378742263 1102 46-47 0.873686403494375 1102 48-49 0.863855220405604 1102 50-51 0.6796578807002831 1102 52-53 0.5307205054787403 1102 54-55 0.6513523716470928 1102 56-57 0.738909193635088 1102 58-59 0.7188585153867706 1102 60-61 0.6097656481461371 1102 62-63 0.7110419297305377 1102 64-65 0.7289769799383343 1102 66-67 0.5633079486731098 1102 68-69 0.6104597323993701 1102 70-71 0.8288505953030203 1102 72-73 0.8434524596493951 1102 74-75 0.8362533758545112 1102 76-77 1.1014040984813747 1102 78-79 1.2089950333888844 1102 80-81 1.3338739311768748 1102 82-83 1.562065572728585 1102 84-85 1.532151318771053 1102 86-87 1.5409742222568887 1102 88-89 1.4125379428493474 1102 90-91 1.4568406625260089 1102 92-93 1.5035448842385861 1102 94-95 1.4220077591866165 1102 96-97 1.4896629100026537 1102 98-99 1.582307051942184 1102 100-101 1.4129283240796795 1103 1 0.5083364926295495 1103 2 0.48046373287412436 1103 3 0.4565807349018911 1103 4 0.3720590675491877 1103 5 0.3028093261613094 1103 6 0.47683126791744 1103 7 0.36281904073987903 1103 8 0.4499134619837264 1103 9 0.5913888596056012 1103 10-11 0.6796583924786859 1103 12-13 0.7389625055505036 1103 14-15 0.8042292347508564 1103 16-17 0.848717359998524 1103 18-19 0.9199992946720315 1103 20-21 0.738102283874909 1103 22-23 0.6797079629877842 1103 24-25 0.799904150686487 1103 26-27 0.7168011785282573 1103 28-29 0.8052298253175678 1103 30-31 0.8557667451361226 1103 32-33 0.8424140032383676 1103 34-35 0.8755660137483545 1103 36-37 1.0046971685649595 1103 38-39 0.9392391389076948 1103 40-41 1.1064113956162203 1103 42-43 0.9446980376404426 1103 44-45 0.8713914120485811 1103 46-47 0.7321938216290462 1103 48-49 0.8141412855760493 1103 50-51 0.9610676236191225 1103 52-53 0.9238801854332124 1103 54-55 0.9496649121658294 1103 56-57 1.1049694930889373 1103 58-59 1.007012295347316 1103 60-61 1.088825115071188 1103 62-63 1.1418715697376278 1103 64-65 1.4458113037943612 1103 66-67 1.39715155569154 1103 68-69 1.4158300386393705 1103 70-71 1.433252285196538 1103 72-73 1.4205176324282682 1103 74-75 1.3775268796347042 1103 76-77 1.2269048983619761 1103 78-79 1.279755243526612 1103 80-81 1.2704558466460512 1103 82-83 1.3082342204406459 1103 84-85 1.7396437426424747 1103 86-87 1.7145357780829578 1103 88-89 1.5905908836348637 1103 90-91 1.5641054090128286 1103 92-93 1.523824218635312 1103 94-95 1.2945280272981954 1103 96-97 1.5870935559237296 1103 98-99 1.4228095775127088 1103 100-101 1.3034327152609375 1104 1 0.3906778258964607 1104 2 0.3609825592184137 1104 3 0.2657156875955877 1104 4 0.1524516884027065 1104 5 0.3112664778749803 1104 6 0.30314954100564506 1104 7 0.3613426302343825 1104 8 0.4836875458522627 1104 9 0.5501891324973016 1104 10-11 0.5734133656254201 1104 12-13 0.6115369591789843 1104 14-15 0.6116667789469972 1104 16-17 0.6622004516455675 1104 18-19 0.6683631207464842 1104 20-21 0.6720649840635247 1104 22-23 0.676482527046069 1104 24-25 0.794655067821239 1104 26-27 0.6942860912984017 1104 28-29 0.774462462721722 1104 30-31 0.8572888221020989 1104 32-33 0.6327000551791215 1104 34-35 0.6382756124232003 1104 36-37 0.8741063829496198 1104 38-39 0.9086287028760225 1104 40-41 0.7642831171449131 1104 42-43 0.8791375371913901 1104 44-45 0.7741921499078757 1104 46-47 0.7076573707986782 1104 48-49 0.6166970159557295 1104 50-51 0.622208925334661 1104 52-53 0.882909828501397 1104 54-55 0.9505125093488971 1104 56-57 0.9929897966740313 1104 58-59 0.9035807376089906 1104 60-61 0.8948952947341127 1104 62-63 0.918175035821978 1104 64-65 1.0444917506614395 1104 66-67 1.0958247374399015 1104 68-69 0.9996072361302062 1104 70-71 1.179276673854094 1104 72-73 1.2006241459877458 1104 74-75 1.1145759406354117 1104 76-77 1.1157576461682552 1104 78-79 1.349572550008066 1104 80-81 1.376689526075804 1104 82-83 1.2184156269953057 1104 84-85 1.5062410793191496 1104 86-87 1.8088703693197026 1104 88-89 1.6319734841598859 1104 90-91 1.6251777266964034 1104 92-93 1.6896738016175021 1104 94-95 1.7592334578706463 1104 96-97 1.9547914542978653 1104 98-99 2.147986742621871 1104 100-101 2.0097367625547857 1105 1 0.3968225145411459 1105 2 0.2190777973136484 1105 3 0.29104535792525965 1105 4 0.18373373968475448 1105 5 0.33562545223394835 1105 6 0.37068617104227286 1105 7 0.25115948005122846 1105 8 0.386187545852259 1105 9 0.4447678870760541 1105 10-11 0.5297961495082077 1105 12-13 0.5304884243804509 1105 14-15 0.7166026763828981 1105 16-17 0.5176491695942858 1105 18-19 0.5891369302702856 1105 20-21 0.5208470353455823 1105 22-23 0.4675081680717099 1105 24-25 0.6522191703853366 1105 26-27 0.6126560546683635 1105 28-29 0.5360421330513887 1105 30-31 0.4732549393182168 1105 32-33 0.6385288097578723 1105 34-35 0.7233442937418815 1105 36-37 0.7636301924734283 1105 38-39 0.7245352962826175 1105 40-41 0.7565724944342875 1105 42-43 0.7362300280338872 1105 44-45 0.8865456297613505 1105 46-47 0.8025245869158937 1105 48-49 0.7036292503879693 1105 50-51 0.7246997678255056 1105 52-53 0.8462385830801509 1105 54-55 0.798159029495416 1105 56-57 0.7561170860513187 1105 58-59 0.807092642370904 1105 60-61 0.8321984082872262 1105 62-63 0.9494799808769265 1105 64-65 1.046813179232867 1105 66-67 0.981085726450889 1105 68-69 1.0532198734928464 1105 70-71 1.1117308863083082 1105 72-73 1.1566909958045954 1105 74-75 1.0503131201225884 1105 76-77 1.2524380490986573 1105 78-79 1.3451403155758328 1105 80-81 1.4787637018999789 1105 82-83 1.4775593998890777 1105 84-85 1.4446568302348943 1105 86-87 1.6158529700523054 1105 88-89 1.3554579163943217 1105 90-91 1.3443627083813858 1105 92-93 1.4604430323867348 1105 94-95 1.7303598314970223 1105 96-97 2.0026302821366926 1105 98-99 2.0633850942702274 1105 100-101 2.0254922570602805 1106 1 0.18676471107293935 1106 2 0.12569835883305558 1106 3 0.04210440003921434 1106 4 0.15401243497790063 1106 5 0.16844956453783055 1106 6 0.14586164585647765 1106 7 0.2818345419835211 1106 8 0.21685228573664972 1106 9 0.15934055429323024 1106 10-11 0.15065907271216616 1106 12-13 0.1494520907718595 1106 14-15 0.27239747407075754 1106 16-17 0.2348095742185663 1106 18-19 0.08139642655434898 1106 20-21 0.13623411214162218 1106 22-23 0.2858349641080409 1106 24-25 0.12853110267270296 1106 26-27 0.012739663256311928 1106 28-29 0.15790113387715365 1106 30-31 -0.15128985836964404 1106 32-33 0.07236861157455365 1106 34-35 -0.156824987843585 1106 36-37 0.04011243855104851 1106 38-39 0.20296944161705 1106 40-41 0.4182291831213263 1106 42-43 -0.0670916069785008 1106 44-45 0.1405134249719211 1106 46-47 0.11172875702324347 1106 48-49 0.4081121157884624 1106 50-51 -0.1163128250729315 1106 52-53 -0.05691686696114573 1106 54-55 0.11816109390499463 1106 56-57 -0.599574491157604 1106 58-59 -0.29820153599408883 1106 60-61 0.254897623811587 1106 62-63 0.18477725585628235 1106 64-65 0.24609166808505378 1106 66-67 -0.05378421574564385 1106 68-69 0.005983085714149183 1106 70-71 -0.09191382880316823 1106 72-73 0.2051478496443977 1106 74-75 0.47570535794257296 1106 76-77 0.4191938294454829 1106 78-79 0.6193517111167068 1106 80-81 0.6529214227918061 1106 82-83 0.6745102669411018 1106 84-85 0.5938362274272997 1106 86-87 0.4796070988714618 1106 88-89 0.4542213350565838 1106 90-91 0.42937509483885705 1106 92-93 0.4437811826757496 1106 94-95 0.24935446403211614 1106 96-97 0.5503119501796334 1106 98-99 0.922297150422164 1106 100-101 0.26777962287365575 1107 1 0.3432328158726108 1107 2 0.22148244242326953 1107 3 0.2581496726714789 1107 4 0.24917372366713408 1107 5 0.28836846955299933 1107 6 0.3309937593894574 1107 7 0.28274822767361485 1107 8 0.2374366698887016 1107 9 0.3283237985787082 1107 10-11 0.42913016692737216 1107 12-13 0.4577425288954089 1107 14-15 0.5966639938320881 1107 16-17 0.688698573939071 1107 18-19 0.6212846677815449 1107 20-21 0.5976237397203903 1107 22-23 0.6431613866121069 1107 24-25 0.7596528474300897 1107 26-27 0.5601387856724642 1107 28-29 0.6345058181049481 1107 30-31 0.864448877650382 1107 32-33 0.8061345510733204 1107 34-35 0.8128969266380679 1107 36-37 0.5931857035355961 1107 38-39 0.6055191284980594 1107 40-41 0.684895149354702 1107 42-43 0.8453979877896174 1107 44-45 0.6690321148950034 1107 46-47 0.7256766792174218 1107 48-49 0.7036570309465802 1107 50-51 0.652726597337967 1107 52-53 0.7869079443775746 1107 54-55 0.9272612919841592 1107 56-57 0.9779322076851145 1107 58-59 0.9781193968007713 1107 60-61 0.877609485472135 1107 62-63 1.067074209528439 1107 64-65 1.1792199516825583 1107 66-67 1.2049828883289528 1107 68-69 1.458873967996798 1107 70-71 1.5766943461140919 1107 72-73 1.6788972977367216 1107 74-75 1.4770143664935986 1107 76-77 1.5496270820348883 1107 78-79 1.5469302845416948 1107 80-81 1.50966657005495 1107 82-83 1.4970206822997305 1107 84-85 1.4699158651733306 1107 86-87 1.6546575300683237 1107 88-89 1.6936853415620057 1107 90-91 1.5833368299150727 1107 92-93 1.6973526329473465 1107 94-95 1.7457640261110932 1107 96-97 1.8195859333530287 1107 98-99 1.9108874718856015 1107 100-101 1.716921203901805 1108 1 0.3872819935570675 1108 2 0.1619473445536741 1108 3 0.27799284603772634 1108 4 0.28461031607508147 1108 5 0.14890847890337255 1108 6 0.2807879903517616 1108 7 0.24388637688811343 1108 8 0.3940348685729518 1108 9 0.32449085585835746 1108 10-11 0.40492897977490117 1108 12-13 0.32139937124834006 1108 14-15 0.4812083203771067 1108 16-17 0.577182454688355 1108 18-19 0.578765574057762 1108 20-21 0.5218430039211484 1108 22-23 0.3283826770648872 1108 24-25 0.6151073241996841 1108 26-27 0.6435615746187437 1108 28-29 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68-69 -1.8441002064443843 2316 70-71 -2.008095017276606 2316 72-73 -2.0557406529620152 2316 74-75 -2.1434828560433665 2316 76-77 -2.4257506738673555 2316 78-79 -2.4683352373641902 2316 80-81 -2.621913741579988 2316 82-83 -2.6478598414428802 2316 84-85 -2.667613901950869 2316 86-87 -2.6497663595538974 2316 88-89 -2.8313513989899164 2316 90-91 -2.7385470312096096 2316 92-93 -3.0433915024570695 2316 94-95 -3.3437499473269696 2316 96-97 -3.346441367120331 2316 98-99 -3.3870305915715697 2316 100-101 -3.1976948720653144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9968.0 3 3474.0 4 3747.0 5 4019.0 6 4932.0 7 6049.0 8 7722.0 9 9830.0 10 12118.0 11 14968.0 12 17915.0 13 20871.0 14 23596.0 15 26144.0 16 29625.0 17 33456.0 18 33169.0 19 33008.0 20 35144.0 21 38180.0 22 42283.0 23 46650.0 24 52307.0 25 58323.0 26 65752.0 27 74013.0 28 84404.0 29 96165.0 30 111417.0 31 129040.0 32 150679.0 33 179336.0 34 217102.0 35 264582.0 36 310240.0 37 300826.0 38 133986.0 39 6328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08204121308688 18.357570496851945 14.527917823530462 24.032470466530718 2 15.43484365788294 19.395572615905927 40.49771340471156 24.671870321499576 3 19.405154263352504 23.050672182006203 29.383058186336143 28.16111536830515 4 12.9753278344272 16.06400783283504 36.79598507684221 34.16467925589555 5 14.213145642290176 36.75694826153441 34.32124687070984 14.708659225465565 6 33.17239119576338 35.612297308304115 17.100802565949767 14.114508929982733 7 29.252044782093705 31.910642455723178 20.2939164039338 18.543396358249318 8 26.459638409805937 35.14861843853543 19.41046935366078 18.981273797997854 9 26.04828084728409 16.813523542998794 19.784258087534354 37.35393752218277 10-11 25.090889962316844 26.173465205487805 27.4439264835478 21.291718348647557 12-13 26.064307052354575 23.256150272476955 27.613922729450096 23.065619945718375 14-15 22.67295301203991 24.98629019954148 25.63787506362444 26.702881724794175 16-17 22.674353020322563 28.036922745269667 26.441781353344613 22.846942881063157 18-19 22.66794357825045 26.798528293113282 28.36756468639437 22.1659634422419 20-21 23.091249506925717 26.065031153371848 28.567878064821468 22.275841274880964 22-23 22.71223747158747 26.036347593351334 28.388127163984166 22.863287771077026 24-25 22.639386638012674 26.18479780775394 28.53655902646312 22.639256527770264 26-27 22.384859655583224 26.565236327621434 28.23266916214944 22.817234854645903 28-29 22.41573524518706 26.93771295055365 28.117744810651757 22.528806993607535 30-31 22.64467794100724 26.485708586300166 28.037949068218825 22.83166440447377 32-33 22.331169930729853 26.428028805466813 28.05947489112819 23.181326372675144 34-35 22.955031029205173 26.59648016549405 27.922826947140024 22.525661858160756 36-37 23.06859705037033 26.269927807644677 27.858966347038567 22.802508794946423 38-39 22.5211344010196 26.534296464971874 28.000923262364662 22.94364587164386 40-41 23.06102999145459 26.353261845280862 27.925483573966492 22.660224589298053 42-43 22.73850358482722 26.392390231101903 27.820299791502233 23.04880639256864 44-45 22.754873060929192 26.608062729523517 27.969253555977264 22.667810653570022 46-47 23.065211696002052 26.711451669543536 27.41003825708239 22.813298377372025 48-49 22.87473768014024 26.81068286306973 27.322511990769687 22.992067466020348 50-51 22.95714798243937 26.95904904011637 27.326050528522643 22.757752448921618 52-53 23.1793892115683 26.670142326795908 27.354027384015172 22.796441077620617 54-55 22.79491768287245 26.81402765308489 27.509888311502994 22.881166352539672 56-57 22.81850662616564 26.95974169817803 27.330631571886695 22.891120103769637 58-59 22.826880717818092 26.596046336787172 27.415368766632938 23.161704178761795 60-61 22.5540981705631 26.803867000859267 27.636003603571464 23.00603122500617 62-63 22.68798096807314 26.800955906916872 27.630109655796957 22.88095346921303 64-65 22.48212957597213 26.83327335185482 27.507196383943644 23.177400688229405 66-67 22.42982788133907 26.840269861964316 27.455506238295825 23.274396018400793 68-69 22.86648017165073 26.589642094561 27.547095070095416 22.996782663692855 70-71 22.742577470060297 26.51772483163854 27.295705505054652 23.443992193246512 72-73 22.72064774318477 26.685119988403954 27.279398715329172 23.314833553082103 74-75 22.746779680230585 26.97233726037504 27.168729610216563 23.11215344917781 76-77 22.8945132656535 26.631615173404178 27.18252965390522 23.291341907037108 78-79 22.848136318869567 26.818938403203457 27.20643931124905 23.126485966677933 80-81 23.00211446342402 26.797911658494744 26.95077876202542 23.24919511605582 82-83 22.976077187957628 26.88504409004618 27.04887453426858 23.090004187727615 84-85 22.96284144315003 26.899990487599695 27.010144826294653 23.127023242955623 86-87 23.08161106561999 26.916261473655812 26.803143736634855 23.198983724089338 88-89 23.03164770928928 26.873111611222512 26.71231107284179 23.38292960664642 90-91 22.98731739083407 26.908031848324605 26.72867036850226 23.375980392339066 92-93 23.13413212044062 26.64855999962833 26.874388481855394 23.34291939807566 94-95 22.97664815658798 26.51407065848942 27.017757185139697 23.491523999782903 96-97 23.23537174230625 26.227059999925658 26.953928562135477 23.58363969563262 98-99 23.032153892096616 26.36576782797094 26.651982419786236 23.950095860146206 100-101 23.78863970681195 25.82494463309104 26.85140771016743 23.53500794992957 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 224.0 1 157.0 2 148.5 3 213.5 4 190.5 5 183.5 6 330.5 7 696.5 8 1138.5 9 1333.5 10 1217.5 11 1084.0 12 1116.0 13 1250.0 14 1396.5 15 1554.5 16 1730.5 17 1970.5 18 2177.5 19 2459.0 20 2955.5 21 3651.0 22 4513.0 23 5725.5 24 7207.0 25 9131.0 26 12109.5 27 15703.5 28 19293.5 29 23305.5 30 27530.5 31 33183.5 32 41137.5 33 45262.0 34 47116.5 35 51648.5 36 57520.5 37 63132.5 38 67898.5 39 72200.0 40 76733.0 41 82521.5 42 86642.0 43 89754.0 44 93736.5 45 98993.0 46 102236.0 47 103719.0 48 104896.0 49 104164.0 50 103197.5 51 101388.5 52 99477.5 53 98397.5 54 96763.0 55 90885.0 56 83443.0 57 76117.5 58 69149.0 59 61649.0 60 51416.5 61 42714.5 62 35600.5 63 27347.0 64 19648.0 65 14614.0 66 11041.0 67 8434.5 68 6571.5 69 5091.5 70 3932.5 71 2809.0 72 1983.5 73 1557.0 74 1204.5 75 903.0 76 653.0 77 425.0 78 289.0 79 199.0 80 123.5 81 83.0 82 57.5 83 38.0 84 23.5 85 15.0 86 9.5 87 7.0 88 6.0 89 3.5 90 3.0 91 3.0 92 4.0 93 3.5 94 1.5 95 1.0 96 0.5 97 1.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00683667190811513 2 0.10674868691312374 3 0.11548030592620556 4 0.11878717440350038 5 0.15817234952633755 6 0.09723679556270269 7 0.027458155109223264 8 0.027978336667449418 9 0.024262754108691192 10-11 0.053485810933324614 12-13 0.05601240707328021 14-15 0.028777186917582433 16-17 0.03290148355780406 18-19 0.037991831663302826 20-21 0.05985803502159497 22-23 0.03245561365075308 24-25 0.049844540025741554 26-27 0.05850184738764822 28-29 0.05510208934638444 30-31 0.06942566011039739 32-33 0.05173948713070825 34-35 0.06407522122578554 36-37 0.05465621943933346 38-39 0.03375606754631845 40-41 0.03845627948314761 42-43 0.034610651534832844 44-45 0.007245385989578535 46-47 0.013487564688292349 48-49 0.008750196925875614 50-51 0.008713041100288033 52-53 0.010124962472616158 54-55 0.011592617583325655 56-57 0.015140998926939757 58-59 0.020324236596407474 60-61 0.02615770121365789 62-63 0.011555461757738073 64-65 0.009641936739977586 66-67 0.010719455682017471 68-69 0.011964175839201478 70-71 0.03258565904030961 72-73 0.02955745925492166 74-75 0.031043692278424947 76-77 0.02452284488780427 78-79 0.005517640099755961 80-81 0.005257549320642885 82-83 0.0063536461754765605 84-85 0.00535043888461184 86-87 0.013617610077848886 88-89 0.04007255789620743 90-91 0.03682142315729399 92-93 0.030857913150487037 94-95 0.04837688491503206 96-97 0.044512679053923504 98-99 0.027105174766141233 100-101 0.07564926089631742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2691368.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.89194131306821 #Duplication Level Percentage of deduplicated Percentage of total 1 76.48301380188545 45.042331602672235 2 13.426199402280858 15.813898945133516 3 4.523943525525725 7.992714498266877 4 1.9923764437867628 4.693396664041183 5 1.0690933369734057 3.148049102961503 6 0.6196798864895519 2.189649090481888 7 0.38699900479796906 1.595378587514446 8 0.27380653970736907 1.289999893406453 9 0.1964638538249109 1.041312397463628 >10 0.9488319230293789 9.795026024574177 >50 0.052521749973979945 2.121949212951769 >100 0.023340284170594562 2.546620774512045 >500 0.0018967360444721976 0.7899226182460393 >1k 0.0017702869748120282 1.7365637988176843 >5k 6.322453481471529E-5 0.2031867889566265 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5457 0.2027593402314362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4167 0.15482832522345513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3028 0.11250783987919898 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.431165117516445E-5 2 3.7155825587582225E-5 0.0 0.0 0.0 7.431165117516445E-5 3 3.7155825587582225E-5 0.0 0.0 0.0 7.431165117516445E-5 4 3.7155825587582225E-5 0.0 0.0 3.7155825587582225E-5 7.431165117516445E-5 5 7.431165117516445E-5 0.0 0.0 3.7155825587582225E-5 7.431165117516445E-5 6 7.431165117516445E-5 0.0 0.0 3.7155825587582225E-5 1.1146747676274668E-4 7 7.431165117516445E-5 0.0 0.0 3.7155825587582225E-5 1.1146747676274668E-4 8 7.431165117516445E-5 0.0 0.0 3.7155825587582225E-5 1.1146747676274668E-4 9 7.431165117516445E-5 0.0 0.0 7.431165117516445E-5 1.1146747676274668E-4 10-11 7.431165117516445E-5 0.0 0.0 7.431165117516445E-5 1.1146747676274668E-4 12-13 7.431165117516445E-5 0.0 0.0 1.1146747676274668E-4 2.2293495352549336E-4 14-15 7.431165117516445E-5 0.0 0.0 1.486233023503289E-4 2.2293495352549336E-4 16-17 7.431165117516445E-5 0.0 0.0 2.2293495352549336E-4 2.600907791130756E-4 18-19 7.431165117516445E-5 0.0 0.0 4.087140814634045E-4 2.600907791130756E-4 20-21 7.431165117516445E-5 0.0 0.0 4.458699070509867E-4 2.600907791130756E-4 22-23 7.431165117516445E-5 0.0 0.0 5.759152966075245E-4 2.972466047006578E-4 24-25 1.8577912793791112E-4 0.0 0.0 7.059606861640623E-4 2.972466047006578E-4 26-27 1.8577912793791112E-4 0.0 0.0 0.001133252680421258 3.529803430820311E-4 28-29 2.600907791130756E-4 0.0 0.0 0.0030467776981817425 3.7155825587582224E-4 30-31 3.3440243028824E-4 0.0 0.0 0.01053367655407956 3.9013616866961334E-4 32-33 3.3440243028824E-4 0.0 0.0 0.026120545388070307 4.458699070509867E-4 34-35 3.3440243028824E-4 0.0 0.0 0.04980738420015397 4.8302573263856894E-4 36-37 3.3440243028824E-4 0.0 0.0 0.09233222658514184 4.8302573263856894E-4 38-39 3.3440243028824E-4 0.0 0.0 0.1537136504558277 4.8302573263856894E-4 40-41 3.3440243028824E-4 0.0 0.0 0.21849483236777728 4.8302573263856894E-4 42-43 3.3440243028824E-4 0.0 0.0 0.28944388132726556 5.016036454323601E-4 44-45 3.7155825587582224E-4 0.0 0.0 0.37778185666174224 5.573373838137334E-4 46-47 3.7155825587582224E-4 0.0 0.0 0.48202252534770423 5.944932094013156E-4 48-49 3.7155825587582224E-4 0.0 0.0 0.5987661293438875 6.6880486057648E-4 50-51 3.7155825587582224E-4 0.0 0.0 0.7234796579286074 6.6880486057648E-4 52-53 3.7155825587582224E-4 0.0 0.0 0.8454436554198459 7.802723373392268E-4 54-55 3.7155825587582224E-4 0.0 0.0 0.9782943098082462 7.802723373392268E-4 56-57 3.7155825587582224E-4 0.0 0.0 1.136262302293852 8.360060757206002E-4 58-59 3.7155825587582224E-4 0.0 0.0 1.307755015293338 8.917398141019735E-4 60-61 3.7155825587582224E-4 0.0 0.0 1.4884623730385438 8.917398141019735E-4 62-63 3.7155825587582224E-4 0.0 0.0 1.6753561757440827 9.288956396895557E-4 64-65 3.7155825587582224E-4 0.0 0.0 1.872857966654876 9.474735524833467E-4 66-67 3.7155825587582224E-4 0.0 0.0 2.108370167141766 9.660514652771379E-4 68-69 4.458699070509867E-4 0.0 0.0 2.3593020352475023 9.660514652771379E-4 70-71 4.458699070509867E-4 0.0 0.0 2.6275856739026398 0.00100320729086472 72-73 4.8302573263856894E-4 0.0 0.0 2.9063286774606816 0.0010960968548336756 74-75 4.8302573263856894E-4 0.0 0.0 3.1905521652928917 0.001151830593215049 76-77 4.8302573263856894E-4 0.0 0.0 3.5021409186703565 0.0011889864188026312 78-79 4.8302573263856894E-4 0.0 0.0 3.8483774794082413 0.0011889864188026312 80-81 5.201815582261512E-4 0.0 0.0 4.212393102689784 0.0011889864188026312 82-83 5.201815582261512E-4 0.0 0.0 4.588855927543168 0.0012447201571840045 84-85 5.201815582261512E-4 0.0 0.0 4.973214365333912 0.0012632980699777956 86-87 5.387594710199422E-4 0.0 0.0 5.3660629092714185 0.001300453895565378 88-89 5.573373838137334E-4 0.0 0.0 5.791942982156287 0.00133760972115296 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3780 0.0 51.336555 1 GTATCAA 6755 0.0 40.978497 1 ATCAACG 8295 0.0 33.086445 3 TCAACGC 8380 0.0 32.750843 4 TATCAAC 8555 0.0 32.358894 2 CAACGCA 8600 0.0 31.968342 5 AACGCAG 8970 0.0 30.784132 6 ACGCAGA 10285 0.0 26.430826 7 CGCAGAG 10420 0.0 26.08742 8 GCAGAGT 11315 0.0 22.845394 9 GTACATG 7485 0.0 22.684792 1 TACATGG 7200 0.0 22.197165 2 AGAGTAC 10005 0.0 20.826242 10-11 GAGTACT 6965 0.0 20.737593 12-13 CAGAGTA 10715 0.0 20.244497 10-11 GTGTAAG 1225 0.0 20.189503 1 ACATGGG 7810 0.0 19.854422 3 CCGTATA 170 7.8752724E-5 19.585733 2 GTACTTT 7355 0.0 19.570469 14-15 TAAGGTG 1580 0.0 19.266977 5 >>END_MODULE