FastQCFastQC Report
Thu 26 May 2016
SRR938403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691368
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55810.2073666626042964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45660.16965349963290044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33120.12306009434607233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36450.049.4216421
GTATCAA61300.039.621961
TCAACGC81250.029.6427084
ATCAACG81500.029.4352053
TATCAAC84350.028.7940832
CAACGCA83550.028.656125
AACGCAG88350.027.0876716
TGGTATC19200.024.7551862
GTGGTAT21500.023.6548861
ACGCAGA102500.023.25567
CGCAGAG103250.022.9946928
TAAGGTG15200.021.564475
GCAGAGT116100.020.2860349
TACATGG69500.020.037812
GTAAGGT14950.020.018554
AGAGTAC103150.019.95572510-11
GTACATG74550.019.5733991
CAGAGTA108800.018.43934610-11
GAGTACT72400.018.4295812-13
ACATGGG74500.018.1090353
Produced by FastQC (version 0.11.5)