FastQCFastQC Report
Thu 26 May 2016
SRR938402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666316
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55050.20646465010148835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42240.15842083233945264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.10797669893591007No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.047.4781531
GTATCAA60700.042.278781
ATCAACG79850.031.9022063
TATCAAC80400.031.6839662
TCAACGC80400.031.6248534
CAACGCA82550.030.6848965
AACGCAG85250.029.8195156
ACGCAGA100150.025.1430037
CGCAGAG101600.024.6438838
TGGTATC19050.023.2016452
GTGGTAT22400.022.5309871
GTACATG75700.022.2024651
TACATGG74500.022.2000432
GCAGAGT111450.021.739519
ACATGGG75200.020.6662043
TATACCG1404.837424E-420.36755
TAAGGTG15150.020.0762165
AGAGTAC99650.019.92683410-11
GAGTACT68300.019.33476612-13
TCGCGTT757.739652E-418.99504958-59
Produced by FastQC (version 0.11.5)