##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938402_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2666316 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.04868102655499 31.0 31.0 34.0 28.0 34.0 2 31.150213628092093 31.0 31.0 34.0 27.0 34.0 3 31.222796922795347 31.0 31.0 34.0 27.0 34.0 4 34.84709014235372 37.0 35.0 37.0 32.0 37.0 5 34.86383646949574 37.0 35.0 37.0 32.0 37.0 6 34.891026419974224 37.0 35.0 37.0 32.0 37.0 7 34.80538765847709 37.0 35.0 37.0 32.0 37.0 8 34.76659293197056 37.0 35.0 37.0 32.0 37.0 9 36.27360372889035 39.0 35.0 39.0 32.0 39.0 10-11 36.133432796412734 39.0 35.0 39.0 31.0 39.0 12-13 35.98824839216357 38.5 35.0 39.0 30.0 39.0 14-15 36.99622212820986 39.0 36.0 41.0 31.0 41.0 16-17 36.84506806395041 39.0 36.0 41.0 30.5 41.0 18-19 36.8240677399078 39.0 36.0 41.0 30.0 41.0 20-21 36.902701517749584 39.0 36.0 41.0 30.0 41.0 22-23 36.82196052530908 39.0 36.0 41.0 30.0 41.0 24-25 36.71138998528306 39.0 36.0 41.0 30.0 41.0 26-27 36.52898399889585 39.0 36.0 41.0 30.0 41.0 28-29 36.352728633815346 39.0 35.5 41.0 29.5 41.0 30-31 36.13488086183333 39.0 35.0 40.0 29.0 41.0 32-33 35.86849852005539 38.5 35.0 40.0 27.0 41.0 34-35 35.62617090397387 38.0 35.0 40.0 27.0 41.0 36-37 35.339769929745756 38.0 34.0 40.0 26.0 41.0 38-39 35.09303435901821 38.0 34.0 40.0 25.0 41.0 40-41 35.043939653064385 38.0 34.0 40.0 25.0 41.0 42-43 34.88237421970989 38.0 34.0 40.0 24.5 41.0 44-45 34.620435274738625 38.0 33.0 40.0 24.0 41.0 46-47 34.29390196060782 38.0 33.0 40.0 23.5 41.0 48-49 33.95764774317823 38.0 33.0 40.0 22.5 41.0 50-51 33.21968813899028 37.5 32.0 39.5 21.0 40.5 52-53 33.28439258512495 37.0 32.5 39.5 21.0 40.5 54-55 33.42138516214882 37.5 32.5 40.0 19.5 41.0 56-57 33.12344767086872 37.0 32.0 40.0 18.5 41.0 58-59 32.79389539724474 37.0 31.5 40.0 17.0 41.0 60-61 32.34620615110887 36.0 31.0 39.0 13.5 41.0 62-63 31.827566387479955 36.0 30.5 39.0 8.5 40.5 64-65 31.32906958515045 35.0 30.0 39.0 7.5 40.0 66-67 30.79412361475534 35.0 29.0 38.0 4.5 40.0 68-69 30.23679207565795 34.5 28.5 38.0 2.0 40.0 70-71 29.61475740309851 34.0 28.0 37.0 2.0 39.0 72-73 29.057901801586908 34.0 27.5 36.5 2.0 39.0 74-75 28.452496440781964 34.0 26.0 36.0 2.0 39.0 76-77 27.724032335252083 33.0 25.5 35.5 2.0 38.0 78-79 26.95078621588739 32.5 24.5 35.0 2.0 37.0 80-81 26.298451496371772 32.0 22.5 35.0 2.0 36.5 82-83 25.641284641430346 31.5 20.5 35.0 2.0 36.0 84-85 24.98861481534822 31.0 18.5 34.0 2.0 35.5 86-87 24.313152304528046 31.0 14.0 34.0 2.0 35.0 88-89 23.529909433090452 30.5 7.0 34.0 2.0 35.0 90-91 22.699874658517594 30.0 2.0 34.0 2.0 35.0 92-93 21.85707695561966 29.0 2.0 34.0 2.0 35.0 94-95 20.907776310084778 29.0 2.0 34.0 2.0 35.0 96-97 19.681356973442007 26.5 2.0 33.5 2.0 35.0 98-99 18.21697371954412 24.5 2.0 33.0 2.0 34.5 100-101 14.799344676324937 8.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.590393393683577 1101 2 0.5443153063279986 1101 3 0.5685936671685212 1101 4 0.5437360475273607 1101 5 0.5266614194857553 1101 6 0.586847585527714 1101 7 0.5322233581543756 1101 8 0.5236440458391911 1101 9 0.6150999709465452 1101 10-11 0.628504988550155 1101 12-13 0.6760889425412628 1101 14-15 0.7852577534901215 1101 16-17 0.660052963574671 1101 18-19 0.6539814223935281 1101 20-21 0.6880840069547318 1101 22-23 0.8960028154991377 1101 24-25 0.9322599903124384 1101 26-27 0.9164689966039674 1101 28-29 0.8519182629511519 1101 30-31 0.8560771655460755 1101 32-33 0.9029589679856898 1101 34-35 0.9299786830104608 1101 36-37 0.8457162718307032 1101 38-39 0.8870554458958466 1101 40-41 0.86736326579733 1101 42-43 0.8119989936023373 1101 44-45 0.882442635745079 1101 46-47 0.9027248258853788 1101 48-49 1.065061327378487 1101 50-51 1.0605831815950992 1101 52-53 1.1090155771754766 1101 54-55 1.1005832509763636 1101 56-57 0.9846985928930039 1101 58-59 0.9414294073180329 1101 60-61 0.9680791505931623 1101 62-63 0.8541029596740088 1101 64-65 0.8182385420452505 1101 66-67 0.7797314888522848 1101 68-69 0.7092256983898793 1101 70-71 0.851026340058521 1101 72-73 0.7757892729038574 1101 74-75 0.7440908996091053 1101 76-77 1.0725169656833984 1101 78-79 1.0255765190917039 1101 80-81 1.1218941926420776 1101 82-83 1.1069360294362838 1101 84-85 1.2367114929170668 1101 86-87 1.3025076097565815 1101 88-89 1.4072258838369471 1101 90-91 1.2509488701720741 1101 92-93 1.229835669684821 1101 94-95 1.0002977350888607 1101 96-97 1.428360300796573 1101 98-99 1.3488864080697525 1101 100-101 1.2618695370249622 1102 1 0.49028873643606374 1102 2 0.6485922586115969 1102 3 0.6391588163051019 1102 4 0.6939459704947595 1102 5 0.6148947808088181 1102 6 0.5961468687472617 1102 7 0.543599357570244 1102 8 0.6118536767398552 1102 9 0.6873493716012575 1102 10-11 0.7114619817230974 1102 12-13 0.7142094322398336 1102 14-15 0.8774358411831642 1102 16-17 0.8932646024341437 1102 18-19 0.8741836177618367 1102 20-21 0.7484681964086874 1102 22-23 0.7555251038420181 1102 24-25 0.6159386317153306 1102 26-27 0.6968308551219735 1102 28-29 0.5697763185168512 1102 30-31 0.3932046283379904 1102 32-33 0.6261238153199713 1102 34-35 0.6788268448154327 1102 36-37 0.6618334879479235 1102 38-39 0.7939098871356691 1102 40-41 0.7702002682190496 1102 42-43 0.7766802149280778 1102 44-45 0.7838688949786459 1102 46-47 0.8688931536816185 1102 48-49 0.7998930239211433 1102 50-51 0.9060753140473636 1102 52-53 0.9564749007975877 1102 54-55 0.9322207718165458 1102 56-57 0.9406050341814947 1102 58-59 0.9302840189301165 1102 60-61 0.9472027678397055 1102 62-63 0.7982196403355459 1102 64-65 0.8648496551845994 1102 66-67 0.8585460021352347 1102 68-69 0.752318015817746 1102 70-71 0.879072048502902 1102 72-73 1.027750075113829 1102 74-75 1.0082570490742597 1102 76-77 0.868610573072683 1102 78-79 0.9024156268278212 1102 80-81 1.1634282123322457 1102 82-83 1.0552371745812827 1102 84-85 1.0884873195267772 1102 86-87 1.0882693563156707 1102 88-89 1.2396358603499138 1102 90-91 1.3803755066585097 1102 92-93 1.3135848938825525 1102 94-95 1.3474080998072004 1102 96-97 1.3898315261382308 1102 98-99 1.494724457063949 1102 100-101 1.3670683249480025 1103 1 0.5630622733317381 1103 2 0.5781235918265786 1103 3 0.5143496559997587 1103 4 0.5975408577661909 1103 5 0.5553616723408581 1103 6 0.6231958065542358 1103 7 0.47727590647846085 1103 8 0.5659367585790136 1103 9 0.42794644835136353 1103 10-11 0.5200852730421062 1103 12-13 0.5140333863199587 1103 14-15 0.7229843943519896 1103 16-17 0.7202795145591239 1103 18-19 0.7875305613679657 1103 20-21 0.7030764572525214 1103 22-23 0.7934844505489238 1103 24-25 0.7924239267032007 1103 26-27 0.7201428854196266 1103 28-29 0.8095197944621688 1103 30-31 0.8294168302434315 1103 32-33 0.8635875705717595 1103 34-35 0.9628273773913278 1103 36-37 0.7772278899835428 1103 38-39 0.7527195800169082 1103 40-41 1.0138685326133938 1103 42-43 0.8706724629900933 1103 44-45 0.9730073055354822 1103 46-47 0.9374031246858223 1103 48-49 0.9608907160922797 1103 50-51 0.9739254826963943 1103 52-53 0.9355905289412689 1103 54-55 1.0429137122272252 1103 56-57 1.0089493149081719 1103 58-59 0.9658763616722936 1103 60-61 1.1149064781184208 1103 62-63 1.219788963964163 1103 64-65 1.1662165999742342 1103 66-67 1.2092708970994366 1103 68-69 1.187861539103146 1103 70-71 1.1478986062873844 1103 72-73 1.0472615846706077 1103 74-75 0.9222815475652908 1103 76-77 0.933327420223403 1103 78-79 0.8855196566520682 1103 80-81 0.9633941866502518 1103 82-83 0.9516452460648068 1103 84-85 0.8285754904244662 1103 86-87 0.997105677991506 1103 88-89 1.1723079119506004 1103 90-91 1.1879513987231292 1103 92-93 1.148743779023686 1103 94-95 0.963074707298766 1103 96-97 1.067469256181429 1103 98-99 1.3005428486847528 1103 100-101 1.1825485308773462 1104 1 0.5694317736131254 1104 2 0.5803898582950708 1104 3 0.5592911414468773 1104 4 0.5445812190989656 1104 5 0.5960527357039709 1104 6 0.5817464349904284 1104 7 0.45688022977316933 1104 8 0.39980593418039234 1104 9 0.5895108287897557 1104 10-11 0.5709709279577666 1104 12-13 0.6717001636183255 1104 14-15 0.7210503837602076 1104 16-17 0.6598821783782256 1104 18-19 0.7074348889917488 1104 20-21 0.7632346124928588 1104 22-23 0.8443906685318723 1104 24-25 0.7154466254434411 1104 26-27 0.737473943587851 1104 28-29 0.8755496073021831 1104 30-31 0.9010206773407887 1104 32-33 0.728676532894454 1104 34-35 0.7702520877046055 1104 36-37 0.7695524545515795 1104 38-39 0.8604296479832527 1104 40-41 0.7964923799993571 1104 42-43 0.7036592304759139 1104 44-45 0.922151232576276 1104 46-47 0.9344626060636116 1104 48-49 0.9794573620756282 1104 50-51 0.884524120483384 1104 52-53 0.9326175423180771 1104 54-55 1.1109164972601988 1104 56-57 1.1533557998502317 1104 58-59 1.052201561170122 1104 60-61 1.123501840244863 1104 62-63 1.2362466329059885 1104 64-65 1.258543329075664 1104 66-67 1.1683612158630545 1104 68-69 1.2245586775899966 1104 70-71 1.275255061647492 1104 72-73 1.2040762573210095 1104 74-75 1.1586197342635138 1104 76-77 1.4354468591763485 1104 78-79 1.2075206956281015 1104 80-81 1.3084821388573538 1104 82-83 1.369918597772621 1104 84-85 1.3384118516995187 1104 86-87 1.4675919043439798 1104 88-89 1.3599246163763503 1104 90-91 1.2739263045659293 1104 92-93 1.4589776929329723 1104 94-95 1.0614253328489163 1104 96-97 1.4493939592795968 1104 98-99 1.2799642689072677 1104 100-101 1.0885832499930626 1105 1 0.4902644675366581 1105 2 0.5419135139345279 1105 3 0.52086613337827 1105 4 0.4678439225947244 1105 5 0.5234394087750545 1105 6 0.647156991135887 1105 7 0.43166667836840844 1105 8 0.4411630290142128 1105 9 0.47136689036027235 1105 10-11 0.5490806100787609 1105 12-13 0.6107372158966982 1105 14-15 0.6463456878658036 1105 16-17 0.6664408369733081 1105 18-19 0.6772307756197407 1105 20-21 0.6680786664634013 1105 22-23 0.7039345737923242 1105 24-25 0.6480725593545031 1105 26-27 0.7375111039064066 1105 28-29 0.677519861155325 1105 30-31 0.6616091316298167 1105 32-33 0.6980740471444022 1105 34-35 0.7529631118636289 1105 36-37 0.7976968540442684 1105 38-39 0.7255179415883077 1105 40-41 0.6460431186295068 1105 42-43 0.6614442139911816 1105 44-45 0.6245279790252312 1105 46-47 0.6347636734593536 1105 48-49 0.5084744785312552 1105 50-51 0.41323005367875965 1105 52-53 0.49846390642176885 1105 54-55 0.5590645026800942 1105 56-57 0.6946035470059186 1105 58-59 0.7957333604245704 1105 60-61 0.7400365992498692 1105 62-63 0.7006899885378033 1105 64-65 0.8305767485888254 1105 66-67 0.9032690685953355 1105 68-69 0.5791628754673148 1105 70-71 0.6858373569577871 1105 72-73 0.49488300095540794 1105 74-75 0.585835125792201 1105 76-77 0.49200162970105765 1105 78-79 0.8615877892714146 1105 80-81 0.8579004766487799 1105 82-83 0.9447110333080673 1105 84-85 0.879142382273173 1105 86-87 0.8671439848745166 1105 88-89 1.037896048349225 1105 90-91 0.9393785628466595 1105 92-93 0.9349574694618283 1105 94-95 1.0600379454739297 1105 96-97 1.152787590964408 1105 98-99 1.150114692500491 1105 100-101 0.9214659341792153 1106 1 0.3583586527334539 1106 2 0.42916489438501415 1106 3 0.25075408956950085 1106 4 0.5340270395064692 1106 5 0.47171694425218647 1106 6 0.15293353587194503 1106 7 0.3227515680748638 1106 8 0.4179956849154536 1106 9 0.4555790213327455 1106 10-11 0.24026466799288926 1106 12-13 0.25446941414815427 1106 14-15 0.5092597221903432 1106 16-17 0.29924430082994036 1106 18-19 0.0855206839338436 1106 20-21 0.49013572185724996 1106 22-23 0.3881880147691632 1106 24-25 0.6744883780884052 1106 26-27 0.39031241421189833 1106 28-29 0.45046660741108013 1106 30-31 0.6056873095695963 1106 32-33 0.5603912171039482 1106 34-35 0.5337974744743192 1106 36-37 0.43726431020296985 1106 38-39 0.5773886408713693 1106 40-41 0.5106783807309299 1106 42-43 0.2554582827833016 1106 44-45 0.37335797699804374 1106 46-47 -0.09927981644423056 1106 48-49 0.44775293941700767 1106 50-51 0.13844452156342157 1106 52-53 0.3031534186545244 1106 54-55 0.1986718842253694 1106 56-57 -0.2707692458730051 1106 58-59 -0.593938210925721 1106 60-61 0.17863847942638955 1106 62-63 0.33900147538071224 1106 64-65 0.47214672502074606 1106 66-67 -0.26099701138771536 1106 68-69 -0.3127865265660006 1106 70-71 -0.13298782577142987 1106 72-73 0.268697082545156 1106 74-75 0.3485012866476822 1106 76-77 0.4710057158022387 1106 78-79 0.5646180588981728 1106 80-81 0.4050510811218224 1106 82-83 0.33074872233528296 1106 84-85 0.5226820111095734 1106 86-87 0.5888172591702592 1106 88-89 0.2711803509777404 1106 90-91 0.2672352847492654 1106 92-93 0.3442614422663226 1106 94-95 0.1556609939022131 1106 96-97 0.40822692077713896 1106 98-99 0.217723495228217 1106 100-101 -0.4452706534450446 1107 1 0.37105077477173154 1107 2 0.37752992781150496 1107 3 0.3568306255617628 1107 4 0.29396227891968607 1107 5 0.3242068914451721 1107 6 0.43306968640820287 1107 7 0.3393151343405236 1107 8 0.3260697307533533 1107 9 0.2782601427723108 1107 10-11 0.3557230469894108 1107 12-13 0.3642317204205696 1107 14-15 0.50520338089418 1107 16-17 0.4726466762134578 1107 18-19 0.4107857030657769 1107 20-21 0.5906143307683607 1107 22-23 0.7177564225412638 1107 24-25 0.5664512598723732 1107 26-27 0.5358305506980372 1107 28-29 0.629513263622691 1107 30-31 0.6796199821764475 1107 32-33 0.6522648614510658 1107 34-35 0.693221476081952 1107 36-37 0.6488484482097263 1107 38-39 0.7022736628622042 1107 40-41 0.708977897408964 1107 42-43 0.6769487603252955 1107 44-45 0.7383183769143429 1107 46-47 0.7801041085942586 1107 48-49 0.6618267138653735 1107 50-51 0.5697915148366874 1107 52-53 0.6268900589349471 1107 54-55 0.6237023378602302 1107 56-57 0.518978703685832 1107 58-59 0.6110740533952566 1107 60-61 0.7259928457939822 1107 62-63 0.66697106732272 1107 64-65 0.6265093635899639 1107 66-67 0.4820434525848043 1107 68-69 0.5295071500578281 1107 70-71 0.5871414571594116 1107 72-73 0.687538663565288 1107 74-75 0.7901856834067544 1107 76-77 0.6916454078683394 1107 78-79 0.6999546855055314 1107 80-81 0.6009435694323919 1107 82-83 0.7217190124320538 1107 84-85 0.7815135652186385 1107 86-87 0.552339449926027 1107 88-89 0.7922604295356876 1107 90-91 0.8421118646610566 1107 92-93 0.7878453068118709 1107 94-95 0.8203100209396545 1107 96-97 1.0258041062397751 1107 98-99 0.8851734280023358 1107 100-101 0.39297833505922597 1108 1 0.3573093854036138 1108 2 0.37046095587440675 1108 3 0.42066324108386155 1108 4 0.4004287858888702 1108 5 0.35569741509571173 1108 6 0.2895337110330587 1108 7 0.38136021371347795 1108 8 0.36133093543505623 1108 9 0.33455524386578617 1108 10-11 0.342371633914901 1108 12-13 0.4974793094436407 1108 14-15 0.603498817104132 1108 16-17 0.4992678952927605 1108 18-19 0.5151783354678159 1108 20-21 0.48249954716673216 1108 22-23 0.5299817821708999 1108 24-25 0.5156590817393365 1108 26-27 0.5824414059897762 1108 28-29 0.4236155858558064 1108 30-31 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1.2825364722862709 1108 98-99 1.2277538869878022 1108 100-101 0.7204908398687682 1109 1 0.40881875713999705 1109 2 0.36488216183274247 1109 3 0.3858203069883288 1109 4 0.39082413025208496 1109 5 0.3780538159092046 1109 6 0.3597001211908051 1109 7 0.3713853607480573 1109 8 0.479295537204969 1109 9 0.3786783956274533 1109 10-11 0.348841910944536 1109 12-13 0.27468929405311115 1109 14-15 0.24894837187795105 1109 16-17 0.41945718512950947 1109 18-19 0.38012426247270525 1109 20-21 0.2370717040402539 1109 22-23 0.39558902121887485 1109 24-25 0.35286054658845956 1109 26-27 0.20300020607772495 1109 28-29 0.2078730612584323 1109 30-31 0.20652466059760854 1109 32-33 0.030426802945754616 1109 34-35 0.20997663536866185 1109 36-37 0.35188265979057576 1109 38-39 0.23317303172616022 1109 40-41 0.2225523313901263 1109 42-43 0.1818137673379212 1109 44-45 0.295571432414242 1109 46-47 0.2967465650158161 1109 48-49 0.41286608473293995 1109 50-51 0.5293374475155161 1109 52-53 0.3774848796121688 1109 54-55 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2316 54-55 -1.1229912785505434 2316 56-57 -0.9317863771633981 2316 58-59 -0.8552385191118006 2316 60-61 -0.903852357052628 2316 62-63 -1.0689720253057402 2316 64-65 -1.1098129919586661 2316 66-67 -1.0609486863517006 2316 68-69 -0.8575882636621763 2316 70-71 -1.1488995277244918 2316 72-73 -1.100617301121762 2316 74-75 -0.9219919152832361 2316 76-77 -0.8904077198603382 2316 78-79 -0.6374935237402433 2316 80-81 -0.7053128155173098 2316 82-83 -0.5312246194386496 2316 84-85 -0.5726422485795339 2316 86-87 -0.5343404628057797 2316 88-89 -0.8102257121860781 2316 90-91 -0.904510265048966 2316 92-93 -0.9353158895143601 2316 94-95 -1.030970555703238 2316 96-97 -1.0347736992421872 2316 98-99 -1.2396691281048327 2316 100-101 -0.9045161055511564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9044.0 3 3196.0 4 3376.0 5 3806.0 6 4451.0 7 5556.0 8 7003.0 9 8794.0 10 10926.0 11 13569.0 12 16650.0 13 19010.0 14 21406.0 15 24834.0 16 27369.0 17 30864.0 18 30311.0 19 29854.0 20 31741.0 21 34657.0 22 38162.0 23 42415.0 24 47484.0 25 53332.0 26 59901.0 27 68228.0 28 78247.0 29 90179.0 30 104683.0 31 122135.0 32 144859.0 33 174235.0 34 213680.0 35 265594.0 36 323097.0 37 329604.0 38 164303.0 39 9761.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.139482847438984 18.390151579198918 14.506904498078255 23.963461075283846 2 15.471834614075618 19.461153902583536 40.40993008982019 24.657081393520652 3 19.41143494813246 23.056421286140864 29.3784600560288 28.153683709697873 4 13.04439191752565 16.074100230517203 36.757418822707386 34.12408902924975 5 14.212133095907786 36.78694935067616 34.301674300435884 14.699243252980171 6 33.22689905068576 35.57779816892502 17.08919500629561 14.10610777409361 7 29.304637997844885 31.902030849949426 20.269516840206386 18.523814311999303 8 26.450573280907648 35.18894258186511 19.40161929345379 18.958864843773448 9 26.04878469746884 16.85919900344442 19.771234963492144 37.320781335594596 10-11 25.119651559799326 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27.89149634835181 22.550442576833206 36-37 23.065250493241706 26.221640947054098 27.886054974405468 22.82705358529873 38-39 22.5699296698531 26.56961679129913 27.931013655235592 22.92943988361218 40-41 23.049475310176142 26.341989535277406 27.918675656436726 22.68985949810973 42-43 22.77510243999059 26.40235779851274 27.782066821297313 23.040472940199354 44-45 22.754032853179744 26.592329782268404 27.963290763936854 22.690346600614998 46-47 23.093748968489027 26.741809427785228 27.37508214578288 22.789359457942858 48-49 22.871874352965182 26.819518170988232 27.307269895664227 23.001337580382355 50-51 22.96905865414532 26.958503004714256 27.3064508476083 22.765987493532123 52-53 23.179881540061718 26.65783712152327 27.36729519975064 22.79498613866437 54-55 22.77210490760888 26.8282706358545 27.532320856353127 22.867303600183494 56-57 22.829486956601645 27.001791581311966 27.307765088113428 22.860956373972954 58-59 22.832568925631307 26.601444294511552 27.40074812242651 23.165238657430635 60-61 22.562349728620674 26.811189755477287 27.624427515275634 23.002033000626405 62-63 22.65576153689492 26.82428633635723 27.653479268488905 22.866472858258945 64-65 22.451417596656107 26.858711431935877 27.508589747364564 23.181281224043452 66-67 22.45807568702089 26.86314079176627 27.419831231937096 23.258952289275744 68-69 22.86585017071023 26.579540840582617 27.56659386905995 22.988015119647194 70-71 22.730101613809005 26.61055467627222 27.24630216443753 23.413041545481246 72-73 22.73276750249601 26.749808825302996 27.26534747841653 23.25207619378446 74-75 22.778022654079816 26.948920470637667 27.174129558528666 23.098927316753855 76-77 22.861120504191813 26.662139233025734 27.255281802736302 23.221458460046147 78-79 22.861618483624742 26.832907819293993 27.218327146595495 23.08714655048577 80-81 23.006589316215916 26.80170989155084 26.956165744107384 23.235535048125865 82-83 22.981870009798726 26.885608060273896 27.078675812145736 23.053846117781642 84-85 22.956029451974384 26.96119266168599 27.03059931264724 23.052178573692387 86-87 23.090835745268855 26.94368120404421 26.828568443987926 23.13691460669901 88-89 23.046621176491776 26.881159294146954 26.74118471542129 23.331034813939983 90-91 22.993485896865412 26.91648651143176 26.780572799848724 23.309454791854105 92-93 23.136294474513857 26.657585820374806 26.95235069122972 23.253769013881616 94-95 22.975381704019185 26.49914684987259 27.08948621903673 23.435985227071495 96-97 23.21429107411921 26.22654697658224 27.03132597563977 23.527835973658775 98-99 23.063917488646585 26.41727294485013 26.68262359334333 23.83618597315995 100-101 23.72166361064809 25.91138885436291 26.84644417249238 23.520503362496626 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 250.0 1 175.0 2 144.0 3 190.0 4 167.5 5 182.5 6 337.5 7 709.0 8 1143.5 9 1343.5 10 1229.5 11 1078.0 12 1150.0 13 1280.0 14 1407.5 15 1602.0 16 1796.5 17 2027.5 18 2253.5 19 2519.0 20 2969.5 21 3632.0 22 4548.5 23 5657.0 24 7114.0 25 9167.0 26 12066.0 27 15665.5 28 19107.0 29 22743.5 30 26988.5 31 32784.0 32 40689.5 33 45044.0 34 46916.5 35 51301.0 36 56838.5 37 62206.0 38 67168.5 39 71512.5 40 75963.0 41 81482.0 42 85443.5 43 88871.0 44 93287.0 45 98086.0 46 100963.0 47 102893.0 48 104312.0 49 103651.5 50 102347.0 51 100255.5 52 98728.0 53 98169.0 54 96442.0 55 90479.5 56 82479.5 57 75090.0 58 68977.0 59 61006.5 60 50465.0 61 42196.0 62 34966.5 63 26601.0 64 19186.0 65 14248.5 66 10786.0 67 8347.5 68 6483.5 69 5012.0 70 3880.5 71 2824.0 72 2029.5 73 1538.0 74 1122.5 75 834.0 76 633.5 77 428.0 78 287.5 79 188.5 80 126.0 81 83.5 82 50.5 83 27.0 84 19.5 85 13.0 86 7.5 87 4.5 88 3.5 89 4.0 90 3.0 91 2.0 92 1.5 93 1.5 94 2.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18508683891931788 2 0.04969403476557167 3 0.047181204328369176 4 0.053969597002005765 5 0.0607954946075409 6 0.06604618507333715 7 0.03855506999170391 8 0.035854714895008695 9 0.04260560263674673 10-11 0.04431207703813052 12-13 0.040880375769413675 14-15 0.036436041339436134 16-17 0.0343545176190669 18-19 0.042830632228138 20-21 0.052731934249353796 22-23 0.04871890653620951 24-25 0.06934661908040908 26-27 0.05408211179770139 28-29 0.04624358103090557 30-31 0.05511349742491138 32-33 0.05140050916695545 34-35 0.05081918272252801 36-37 0.04853138187671679 38-39 0.027941174264415773 40-41 0.026009670271640722 42-43 0.0228405035262137 44-45 0.004519344293774631 46-47 0.01215159793512847 48-49 0.011551519024751754 50-51 0.00958251010007816 52-53 0.010013816816911424 54-55 0.011739043684244478 56-57 0.008832411462107267 58-59 0.011570271490701027 60-61 0.011476509160954666 62-63 0.004088037576941368 64-65 0.007557243777556749 66-67 0.004219304838586274 68-69 0.009319975576788347 70-71 0.023815631755575856 72-73 0.02102151432913428 74-75 0.021415316114069 76-77 0.012995458902845725 78-79 0.004838136214912261 80-81 0.006825897605535128 82-83 0.005757007046426605 84-85 0.005438215125288975 86-87 0.008794906530208722 88-89 0.031204103339589156 90-91 0.03833004040031264 92-93 0.023703116959880224 94-95 0.033754438708690195 96-97 0.037054872715762126 98-99 0.022427949275329706 100-101 0.1224536026487483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2666316.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.76211807701996 #Duplication Level Percentage of deduplicated Percentage of total 1 76.46671218948153 44.933459706398146 2 13.35124523960152 15.690948984894304 3 4.535861396977678 7.996104689705971 4 2.055826265461128 4.832188230274632 5 1.056215621321872 3.103273352745443 6 0.6261240524008087 2.2075425298823137 7 0.42093678282895663 1.7314595854889245 8 0.2586385539053483 1.2158519395084617 9 0.20021176769044668 1.0588380780091424 >10 0.9494409207447828 9.864286493551422 >50 0.05150899720557632 2.059685422024984 >100 0.02343730504416909 2.5293876447533727 >500 0.0020484839126312577 0.8513022191049328 >1k 0.001728408301233365 1.7185915109110126 >5k 6.40151222679024E-5 0.2070796127468454 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5505 0.20646465010148835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4224 0.15842083233945264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2879 0.10797669893591007 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.750493189854466E-5 2 0.0 0.0 0.0 0.0 3.750493189854466E-5 3 0.0 0.0 0.0 0.0 3.750493189854466E-5 4 0.0 0.0 0.0 3.750493189854466E-5 3.750493189854466E-5 5 0.0 0.0 0.0 3.750493189854466E-5 3.750493189854466E-5 6 0.0 0.0 0.0 3.750493189854466E-5 3.750493189854466E-5 7 0.0 0.0 0.0 3.750493189854466E-5 3.750493189854466E-5 8 0.0 0.0 0.0 3.750493189854466E-5 3.750493189854466E-5 9 0.0 0.0 0.0 3.750493189854466E-5 3.750493189854466E-5 10-11 0.0 0.0 0.0 3.750493189854466E-5 3.750493189854466E-5 12-13 0.0 0.0 0.0 5.625739784781699E-5 1.312672616449063E-4 14-15 0.0 0.0 3.750493189854466E-5 7.500986379708932E-5 1.5001972759417863E-4 16-17 0.0 0.0 3.750493189854466E-5 7.500986379708932E-5 1.5001972759417863E-4 18-19 0.0 0.0 3.750493189854466E-5 2.2502959139126795E-4 1.5001972759417863E-4 20-21 0.0 0.0 3.750493189854466E-5 3.5629685303617423E-4 1.5001972759417863E-4 22-23 3.750493189854466E-5 0.0 3.750493189854466E-5 4.8756411468108056E-4 1.6877219354345096E-4 24-25 3.750493189854466E-5 0.0 3.750493189854466E-5 7.313461720216208E-4 1.875246594927233E-4 26-27 3.750493189854466E-5 0.0 3.750493189854466E-5 0.0011814053548041567 2.4378205734054028E-4 28-29 7.500986379708932E-5 0.0 3.750493189854466E-5 0.0034879586665646535 2.625345232898126E-4 30-31 1.1251479569563398E-4 0.0 3.750493189854466E-5 0.01134524189930976 2.625345232898126E-4 32-33 1.312672616449063E-4 0.0 3.750493189854466E-5 0.02696604603505361 2.625345232898126E-4 34-35 1.5001972759417863E-4 0.0 3.750493189854466E-5 0.05061290559708602 2.625345232898126E-4 36-37 1.875246594927233E-4 0.0 3.750493189854466E-5 0.09428739879294126 2.625345232898126E-4 38-39 1.875246594927233E-4 0.0 3.750493189854466E-5 0.1579707731566701 3.0003945518835727E-4 40-41 1.875246594927233E-4 0.0 3.750493189854466E-5 0.22795497607935444 3.0003945518835727E-4 42-43 1.875246594927233E-4 0.0 3.750493189854466E-5 0.2992143466865893 3.0003945518835727E-4 44-45 2.2502959139126795E-4 0.0 3.750493189854466E-5 0.38980750968752387 3.375443870869019E-4 46-47 2.2502959139126795E-4 0.0 3.750493189854466E-5 0.5009158704369625 3.9380178493471894E-4 48-49 2.2502959139126795E-4 0.0 3.750493189854466E-5 0.6163372983547337 4.500591827825359E-4 50-51 2.2502959139126795E-4 0.0 3.750493189854466E-5 0.7430477107739668 4.8756411468108056E-4 52-53 2.2502959139126795E-4 0.0 3.750493189854466E-5 0.8635885618958894 4.8756411468108056E-4 54-55 2.2502959139126795E-4 0.0 3.750493189854466E-5 0.9988876037198893 4.8756411468108056E-4 56-57 2.625345232898126E-4 0.0 3.750493189854466E-5 1.1584523364822474 5.063165806303529E-4 58-59 2.625345232898126E-4 0.0 3.750493189854466E-5 1.334031675165284 5.250690465796252E-4 60-61 2.625345232898126E-4 0.0 3.750493189854466E-5 1.5262256986793763 5.250690465796252E-4 62-63 2.625345232898126E-4 0.0 3.750493189854466E-5 1.714125407491085 6.000789103767145E-4 64-65 2.625345232898126E-4 0.0 3.750493189854466E-5 1.9111388147541402 7.125937060723486E-4 66-67 2.625345232898126E-4 0.0 3.750493189854466E-5 2.1389625235718497 7.125937060723486E-4 68-69 3.0003945518835727E-4 0.0 3.750493189854466E-5 2.392495863206012 7.125937060723486E-4 70-71 3.0003945518835727E-4 0.0 3.750493189854466E-5 2.6630564419221128 7.125937060723486E-4 72-73 3.0003945518835727E-4 0.0 3.750493189854466E-5 2.938080107534141 8.251085017679826E-4 74-75 3.0003945518835727E-4 0.0 3.750493189854466E-5 3.2190107999201896 8.626134336665272E-4 76-77 3.0003945518835727E-4 0.0 3.750493189854466E-5 3.5311455956458273 8.626134336665272E-4 78-79 3.0003945518835727E-4 0.0 3.750493189854466E-5 3.873584376345489 8.626134336665272E-4 80-81 3.375443870869019E-4 0.0 3.750493189854466E-5 4.232900376399496 9.001183655650718E-4 82-83 3.375443870869019E-4 0.0 3.750493189854466E-5 4.608249734840131 9.376232974636165E-4 84-85 3.5629685303617423E-4 0.0 3.750493189854466E-5 4.992018950491989 9.376232974636165E-4 86-87 3.750493189854466E-4 0.0 3.750493189854466E-5 5.3901713075269395 9.376232974636165E-4 88-89 3.750493189854466E-4 0.0 3.750493189854466E-5 5.824872220697022 9.376232974636165E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3540 0.0 47.478153 1 GTATCAA 6070 0.0 42.27878 1 ATCAACG 7985 0.0 31.902206 3 TATCAAC 8040 0.0 31.683966 2 TCAACGC 8040 0.0 31.624853 4 CAACGCA 8255 0.0 30.684896 5 AACGCAG 8525 0.0 29.819515 6 ACGCAGA 10015 0.0 25.143003 7 CGCAGAG 10160 0.0 24.643883 8 TGGTATC 1905 0.0 23.201645 2 GTGGTAT 2240 0.0 22.530987 1 GTACATG 7570 0.0 22.202465 1 TACATGG 7450 0.0 22.200043 2 GCAGAGT 11145 0.0 21.73951 9 ACATGGG 7520 0.0 20.666204 3 TATACCG 140 4.837424E-4 20.3675 5 TAAGGTG 1515 0.0 20.076216 5 AGAGTAC 9965 0.0 19.926834 10-11 GAGTACT 6830 0.0 19.334766 12-13 TCGCGTT 75 7.739652E-4 18.995049 58-59 >>END_MODULE