FastQCFastQC Report
Thu 26 May 2016
SRR938402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666316
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55900.20965256931286466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46010.17256019166520398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.11979075248395164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37100.049.352431
GTATCAA64300.041.714771
TCAACGC80400.033.461524
ATCAACG80150.033.447913
CAACGCA82750.032.5112535
TATCAAC83100.032.449182
AACGCAG86900.030.8255336
CGCAGAG98950.026.7836448
ACGCAGA99350.026.771427
TACATGG69400.023.0936282
GCAGAGT114200.022.7079459
GTACATG72300.022.6277771
GTAAGGT14600.022.4637134
GAGTACT68400.021.80342912-13
TAAGGTG16750.020.7153995
TGGTATC18150.020.7000892
AGAGTAC98200.020.65226410-11
CCGATCG2309.041105E-820.650169
ACATGGG73100.020.5476973
GTACTTT72700.020.25249714-15
Produced by FastQC (version 0.11.5)