##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938402_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2666316 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81267411664634 31.0 31.0 34.0 30.0 34.0 2 32.04307253903888 33.0 31.0 34.0 30.0 34.0 3 32.114451175329556 33.0 31.0 34.0 30.0 34.0 4 35.77367761360619 37.0 35.0 37.0 35.0 37.0 5 35.63794501476944 37.0 35.0 37.0 33.0 37.0 6 35.66224371004787 37.0 35.0 37.0 33.0 37.0 7 35.601653742467136 37.0 35.0 37.0 33.0 37.0 8 35.59317650271011 37.0 35.0 37.0 33.0 37.0 9 37.18690620316572 39.0 37.0 39.0 33.0 39.0 10-11 37.09420601309073 39.0 37.0 39.0 33.0 39.0 12-13 36.98872995548915 39.0 37.0 39.0 33.0 39.0 14-15 38.066989621635244 40.0 37.0 41.0 33.0 41.0 16-17 37.98400189624936 40.0 37.0 41.0 33.0 41.0 18-19 38.08116423559698 40.0 37.5 41.0 33.0 41.0 20-21 38.130923153894734 40.0 37.0 41.0 33.0 41.0 22-23 38.03702336857297 40.0 37.0 41.0 33.0 41.0 24-25 37.94124421111376 40.0 37.0 41.0 33.0 41.0 26-27 37.7537632448667 40.0 37.0 41.0 32.0 41.0 28-29 37.63927006401342 40.0 37.0 41.0 32.0 41.0 30-31 37.41496225503654 39.0 36.5 41.0 31.5 41.0 32-33 37.21492745045973 39.0 36.0 41.0 31.0 41.0 34-35 37.07476833203566 39.0 36.0 41.0 31.0 41.0 36-37 37.07835136570459 39.0 36.0 41.0 31.0 41.0 38-39 36.958067610890836 39.0 36.0 41.0 31.0 41.0 40-41 36.797561691862484 39.0 35.5 41.0 30.0 41.0 42-43 36.60278976685434 39.0 35.0 41.0 30.0 41.0 44-45 36.357310423820735 39.0 35.0 40.0 30.0 41.0 46-47 36.03516406157409 38.5 35.0 40.0 29.5 41.0 48-49 35.7030721414866 38.0 35.0 40.0 28.0 41.0 50-51 35.35693799984698 38.0 34.0 40.0 27.0 41.0 52-53 34.87775642496988 38.0 34.0 40.0 26.0 41.0 54-55 34.56474926452829 38.0 33.0 40.0 26.0 41.0 56-57 34.23126891186191 37.0 33.0 40.0 25.0 41.0 58-59 33.79010402367911 37.0 32.5 40.0 23.5 41.0 60-61 33.41652602317205 36.5 32.0 39.0 23.0 40.5 62-63 32.960845788721215 36.0 31.5 39.0 22.0 40.0 64-65 32.45860843200881 35.0 31.0 39.0 20.5 40.0 66-67 31.961848858124846 35.0 30.5 38.0 19.5 40.0 68-69 31.426838754296192 35.0 30.0 38.0 18.0 40.0 70-71 30.842710691455927 34.0 29.0 37.0 14.5 39.0 72-73 30.383693455689425 34.0 29.0 36.5 12.5 39.0 74-75 29.865642331966654 34.0 29.0 36.0 8.0 39.0 76-77 28.58856864677705 32.5 27.0 34.5 7.5 36.5 78-79 29.29533277375975 33.5 29.0 35.0 7.0 37.0 80-81 29.021357558518943 34.0 28.5 35.0 4.0 37.0 82-83 28.547925677226555 33.0 27.5 35.0 2.0 36.0 84-85 28.018328472694158 33.0 26.5 35.0 2.0 36.0 86-87 27.4460206517157 32.5 26.0 35.0 2.0 35.5 88-89 26.802330068904062 32.0 25.0 34.0 2.0 35.0 90-91 26.25370830014147 32.0 24.5 34.0 2.0 35.0 92-93 25.512602969790528 31.0 22.0 34.0 2.0 35.0 94-95 24.73408534472283 31.0 19.0 34.0 2.0 35.0 96-97 23.731557512312868 31.0 6.5 34.0 2.0 35.0 98-99 22.466739876293733 30.5 2.0 34.0 2.0 35.0 100-101 18.750072572043223 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27420903550735787 1101 2 0.3006904741417955 1101 3 0.2847282585727626 1101 4 0.20102175211972195 1101 5 0.07249110132880787 1101 6 0.1273279441716042 1101 7 0.1592902257533808 1101 8 0.21065372949952632 1101 9 0.3044761718253923 1101 10-11 0.3519626352475882 1101 12-13 0.3378623018822964 1101 14-15 0.4833377843414155 1101 16-17 0.443024537636461 1101 18-19 0.4462925879554547 1101 20-21 0.3723459328905818 1101 22-23 0.40200292822893857 1101 24-25 0.5282742444560498 1101 26-27 0.46206913363609914 1101 28-29 0.5015274331531359 1101 30-31 0.45712096896635046 1101 32-33 0.38824273733119696 1101 34-35 0.3374414736256739 1101 36-37 0.41631737938214997 1101 38-39 0.5276691074678865 1101 40-41 0.5346511505715696 1101 42-43 0.5218369562560241 1101 44-45 0.5430551488099695 1101 46-47 0.5753373802835569 1101 48-49 0.6469288683792342 1101 50-51 0.6430328403166996 1101 52-53 0.6059591828284496 1101 54-55 0.5835995443354207 1101 56-57 0.5359410373604803 1101 58-59 0.6359077338165022 1101 60-61 0.5876844106057604 1101 62-63 0.5509921139456537 1101 64-65 0.7063668568629069 1101 66-67 0.5292644050020314 1101 68-69 0.5301464077151827 1101 70-71 0.4364200973076926 1101 72-73 0.2664326088063831 1101 74-75 0.5996907333259038 1101 76-77 0.42407658844057394 1101 78-79 0.4503575424352526 1101 80-81 0.4645856009579319 1101 82-83 0.45163714094198326 1101 84-85 0.4222117083011163 1101 86-87 0.5478253545654788 1101 88-89 0.5090407408589321 1101 90-91 0.5486233978490631 1101 92-93 0.5167197967978261 1101 94-95 0.6273470745246499 1101 96-97 0.6565008286376965 1101 98-99 0.5072817400194651 1101 100-101 0.3712159135677986 1102 1 0.3561325383610914 1102 2 0.29527811241714375 1102 3 0.3301458531597916 1102 4 0.19319375508906944 1102 5 0.24201393729682508 1102 6 0.20205930362056534 1102 7 0.2723273547373495 1102 8 0.33563152025804754 1102 9 0.3468313249778845 1102 10-11 0.4034996364523664 1102 12-13 0.3925040333512726 1102 14-15 0.38513521172023957 1102 16-17 0.41740876359876466 1102 18-19 0.45760652318966066 1102 20-21 0.4477116247845103 1102 22-23 0.37370470375131504 1102 24-25 0.4612850874336658 1102 26-27 0.4333970028875598 1102 28-29 0.3826949090880518 1102 30-31 0.38713143469110634 1102 32-33 0.28281090449072366 1102 34-35 0.35754600917871926 1102 36-37 0.38461961977741055 1102 38-39 0.4660372454207362 1102 40-41 0.5564362868085269 1102 42-43 0.5639788398430952 1102 44-45 0.5462441524973087 1102 46-47 0.36181529882127705 1102 48-49 0.6273010709275155 1102 50-51 0.5365523053477901 1102 52-53 0.5070303984345443 1102 54-55 0.56848143984854 1102 56-57 0.4601932248186529 1102 58-59 0.49081475923848217 1102 60-61 0.5920395515888046 1102 62-63 0.5067896386800612 1102 64-65 0.48457107235947916 1102 66-67 0.710393851413869 1102 68-69 0.5938221284507073 1102 70-71 0.2758299399567363 1102 72-73 0.1568333487087834 1102 74-75 0.20665561230075014 1102 76-77 0.3017045806034524 1102 78-79 0.3705880317687331 1102 80-81 0.44836251064846167 1102 82-83 0.31386743556430474 1102 84-85 0.3496842357736405 1102 86-87 0.2092098725945135 1102 88-89 0.36811062999526456 1102 90-91 0.1822773961210018 1102 92-93 0.4206222343381363 1102 94-95 0.18566245792309388 1102 96-97 0.12397645931930867 1102 98-99 0.1435357407252944 1102 100-101 0.08807364055841305 1103 1 0.2333945065248919 1103 2 0.2752443826106443 1103 3 0.2328767839841035 1103 4 0.18377307930943942 1103 5 0.15725448407474119 1103 6 0.13508196768353287 1103 7 0.17882478062053764 1103 8 0.16771330024503328 1103 9 0.2699806237529643 1103 10-11 0.32120778486351753 1103 12-13 0.36012193973626694 1103 14-15 0.4806408629422023 1103 16-17 0.42791974057964666 1103 18-19 0.4032305246098602 1103 20-21 0.39195388764030525 1103 22-23 0.3155444575829023 1103 24-25 0.4342391083816466 1103 26-27 0.4925780195157685 1103 28-29 0.5218717834593107 1103 30-31 0.45977774512366665 1103 32-33 0.4743947260186232 1103 34-35 0.4836082022078898 1103 36-37 0.36135345018158205 1103 38-39 0.48266988640541086 1103 40-41 0.461449897081323 1103 42-43 0.5071455952724051 1103 44-45 0.6122391817889863 1103 46-47 0.5365031501734308 1103 48-49 0.6554920881474686 1103 50-51 0.6632639450897884 1103 52-53 0.6519872006121901 1103 54-55 0.6214771792414027 1103 56-57 0.6250284221676097 1103 58-59 0.6266941012113776 1103 60-61 0.5950915073258329 1103 62-63 0.43273052275604584 1103 64-65 0.5838861205871879 1103 66-67 0.5482363273000175 1103 68-69 0.3162199034669797 1103 70-71 0.22082816470376088 1103 72-73 0.3516525687809775 1103 74-75 0.31144879532933345 1103 76-77 0.21949853290832166 1103 78-79 0.24861631746611224 1103 80-81 0.314900471474548 1103 82-83 0.42243746929410975 1103 84-85 0.20121095333089656 1103 86-87 0.30816395271759944 1103 88-89 0.4337165830102343 1103 90-91 0.431299527608072 1103 92-93 0.5099559227220674 1103 94-95 0.48756493735974615 1103 96-97 0.4184554225715722 1103 98-99 0.5120353560871536 1103 100-101 0.15427282394204767 1104 1 0.290090129838358 1104 2 0.2869913053656603 1104 3 0.26209365580043453 1104 4 0.2121508336781872 1104 5 0.09532646202536199 1104 6 0.15856931959336862 1104 7 0.07995033798788853 1104 8 0.2471487899346343 1104 9 0.2947739108133831 1104 10-11 0.315462752467667 1104 12-13 0.22145327207872612 1104 14-15 0.37988580154161156 1104 16-17 0.3350129165273543 1104 18-19 0.495647803358473 1104 20-21 0.3828671764323488 1104 22-23 0.3997473320832512 1104 24-25 0.48443319487640224 1104 26-27 0.4124946128288016 1104 28-29 0.43616203295785994 1104 30-31 0.4412114598958894 1104 32-33 0.36210414462495066 1104 34-35 0.46795141062359846 1104 36-37 0.5097639611557625 1104 38-39 0.6509147384234808 1104 40-41 0.5973277596767446 1104 42-43 0.703523248629601 1104 44-45 0.494013089060374 1104 46-47 0.5300510368673201 1104 48-49 0.5141246080972621 1104 50-51 0.7590380543842983 1104 52-53 0.6624050998603721 1104 54-55 0.5882609712153126 1104 56-57 0.5771254942754283 1104 58-59 0.648591242584267 1104 60-61 0.6403122175813891 1104 62-63 0.6462058254299592 1104 64-65 0.7445980356220403 1104 66-67 0.6243312204442191 1104 68-69 0.5635701538313462 1104 70-71 0.48642254557505993 1104 72-73 0.3490612615471065 1104 74-75 0.3002404806350363 1104 76-77 0.3550156399372817 1104 78-79 0.5123008583685795 1104 80-81 0.6768494173467836 1104 82-83 0.4301432081936589 1104 84-85 0.38276552121787333 1104 86-87 0.43387319816125824 1104 88-89 0.4494451649522553 1104 90-91 0.26380829396833505 1104 92-93 0.35250812405253384 1104 94-95 0.3556907400408811 1104 96-97 0.041696378429520564 1104 98-99 -0.16187083303534777 1104 100-101 -0.38027493287983916 1105 1 0.2391158069303252 1105 2 0.23364216284001316 1105 3 0.2451430453045731 1105 4 0.16680203247694436 1105 5 0.10935420702024601 1105 6 0.25449592934052134 1105 7 0.26212425791290883 1105 8 0.1746382732204097 1105 9 0.2270164741046301 1105 10-11 0.22499602574809074 1105 12-13 0.33587897655922205 1105 14-15 0.4458518356341372 1105 16-17 0.29777346025162643 1105 18-19 0.3263299085318039 1105 20-21 0.31215246652024575 1105 22-23 0.4045070871829921 1105 24-25 0.313590350680677 1105 26-27 0.35221809876946963 1105 28-29 0.2328992193574635 1105 30-31 0.3537465299465339 1105 32-33 0.3340948985871677 1105 34-35 0.492827697729723 1105 36-37 0.4439354202419139 1105 38-39 0.4102850224177672 1105 40-41 0.3857150913112548 1105 42-43 0.5092890161312482 1105 44-45 0.4333251965115039 1105 46-47 0.23217316631873075 1105 48-49 0.13372351062918142 1105 50-51 0.07209481401827844 1105 52-53 0.10364396982884472 1105 54-55 0.11249541704142274 1105 56-57 0.22486370396010358 1105 58-59 0.2885954953762848 1105 60-61 0.18446631454092 1105 62-63 0.3161969413612127 1105 64-65 0.21595809639980246 1105 66-67 0.20980772927357805 1105 68-69 0.05365942805591217 1105 70-71 0.10137620184073626 1105 72-73 0.16190434934936704 1105 74-75 0.13414204570378274 1105 76-77 0.13417241725682416 1105 78-79 0.26559618724771994 1105 80-81 0.3868273757726435 1105 82-83 0.3219419947028115 1105 84-85 0.26748547348751117 1105 86-87 0.32525344937877065 1105 88-89 0.19108777490034612 1105 90-91 0.12752351380258986 1105 92-93 0.3387425657497154 1105 94-95 0.3119039108964543 1105 96-97 0.16909227276484273 1105 98-99 0.03735464958428736 1105 100-101 -0.29632942917516303 1106 1 0.13233798189331836 1106 2 0.12535093712258316 1106 3 0.20049154150211734 1106 4 0.06919709861328016 1106 5 0.043630164839683516 1106 6 -0.00855295062467576 1106 7 0.08550106914083955 1106 8 0.11324364854861813 1106 9 0.11739399888122648 1106 10-11 0.151197242168422 1106 12-13 0.1760743904900366 1106 14-15 0.18761651890080344 1106 16-17 0.07044570267104433 1106 18-19 0.2036595615600305 1106 20-21 0.15898456857437537 1106 22-23 0.2570446210792454 1106 24-25 0.17241747725195467 1106 26-27 0.0381878608679358 1106 28-29 0.23880972250336896 1106 30-31 0.3857158758530872 1106 32-33 0.34851945681344176 1106 34-35 0.14222304993077728 1106 36-37 -0.14346884620909606 1106 38-39 0.06070048841021958 1106 40-41 0.23425538153789915 1106 42-43 0.4027934918074152 1106 44-45 0.33778858587301386 1106 46-47 0.011476652490564732 1106 48-49 0.061368090267286846 1106 50-51 0.19044831258445782 1106 52-53 0.1764239839062398 1106 54-55 -0.14905316906217791 1106 56-57 0.08260027599060038 1106 58-59 0.039083741181165976 1106 60-61 -0.22631805664634186 1106 62-63 -0.1453564572974102 1106 64-65 -0.511567271679997 1106 66-67 -0.25652959104201756 1106 68-69 -0.9608370496377567 1106 70-71 -0.7604458664394684 1106 72-73 -0.3812152697973943 1106 74-75 -0.2541324059741292 1106 76-77 -0.11104117415133885 1106 78-79 -0.08325644855371195 1106 80-81 -0.8082274954202653 1106 82-83 -0.39150510402554417 1106 84-85 -0.17457721178913488 1106 86-87 0.05423609406864216 1106 88-89 0.26347263519375375 1106 90-91 0.1125586185684142 1106 92-93 -0.10723092540674628 1106 94-95 0.06171503827645353 1106 96-97 0.1533382126084426 1106 98-99 -0.22917446045867962 1106 100-101 -0.5602963913882739 1107 1 0.16023465084573374 1107 2 0.18107021261610612 1107 3 0.1673317800763101 1107 4 0.09991228254702378 1107 5 0.010080410436849263 1107 6 0.09775768473758717 1107 7 0.072873686972585 1107 8 0.1009782917335258 1107 9 0.11430254060788059 1107 10-11 0.11184058038399769 1107 12-13 0.04614380198908208 1107 14-15 0.1837427440381063 1107 16-17 0.1889598035326543 1107 18-19 0.22672896684404975 1107 20-21 0.30501181939838773 1107 22-23 0.22034462460109694 1107 24-25 0.2762040345617578 1107 26-27 0.2700972105481725 1107 28-29 0.34783347633855755 1107 30-31 0.26411834446202676 1107 32-33 0.38819567459628956 1107 34-35 0.247596510934585 1107 36-37 0.26522618446206536 1107 38-39 0.34556990032359636 1107 40-41 0.30988035434715044 1107 42-43 0.34727439199200205 1107 44-45 0.45562965104586084 1107 46-47 0.3760294501852073 1107 48-49 0.28391087555259276 1107 50-51 0.2771184251386103 1107 52-53 0.3517535357406061 1107 54-55 0.36987417933776356 1107 56-57 0.3371931813295177 1107 58-59 0.1218899193328511 1107 60-61 0.16955485432753648 1107 62-63 0.37956761578303144 1107 64-65 0.2140931267861319 1107 66-67 0.14479130947403007 1107 68-69 0.07798719145558763 1107 70-71 0.045706648264250305 1107 72-73 0.10539548924391795 1107 74-75 0.17611400950874767 1107 76-77 0.05956469780666396 1107 78-79 0.19609712643370258 1107 80-81 0.254863320633568 1107 82-83 0.18016607544166163 1107 84-85 0.15655660604012311 1107 86-87 0.4003970715681717 1107 88-89 0.31033912190084934 1107 90-91 0.2340580043165268 1107 92-93 -0.05607166739816449 1107 94-95 -0.2573618851191881 1107 96-97 -0.2530090914162031 1107 98-99 -0.15818615714047723 1107 100-101 -0.31560635552756366 1108 1 0.18895959468269297 1108 2 0.18107765964908396 1108 3 0.17110146816974492 1108 4 0.2445465711691952 1108 5 0.15075647691403304 1108 6 0.1832296404525664 1108 7 0.23897069698884366 1108 8 0.1210146580779039 1108 9 0.16284490801966456 1108 10-11 0.2790470741967539 1108 12-13 0.28596805201080855 1108 14-15 0.363941207466965 1108 16-17 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-0.36674216071150667 2316 78-79 -0.3701024649295164 2316 80-81 -0.5700544943964481 2316 82-83 -0.438662198166881 2316 84-85 -0.3815326954432905 2316 86-87 -0.18061622149514278 2316 88-89 -0.022728548053216002 2316 90-91 0.017675862139622467 2316 92-93 0.1324726506459193 2316 94-95 -0.05555406313838773 2316 96-97 0.2101880585154028 2316 98-99 0.21798100548063815 2316 100-101 0.12663337969954824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 19.0 4 40.0 5 79.0 6 157.0 7 410.0 8 1119.0 9 1855.0 10 3129.0 11 4744.0 12 6774.0 13 9608.0 14 12351.0 15 16152.0 16 19443.0 17 23682.0 18 23325.0 19 21560.0 20 21792.0 21 23484.0 22 25532.0 23 29021.0 24 33576.0 25 39896.0 26 47512.0 27 56541.0 28 68381.0 29 81387.0 30 97055.0 31 115739.0 32 140141.0 33 172491.0 34 219667.0 35 288442.0 36 384560.0 37 442700.0 38 223997.0 39 9948.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.92212717567742 19.156762790027816 15.113700804411815 23.807409229882953 2 15.982627855769477 20.20584955248792 39.25625788943607 24.555264702306527 3 19.41986186079865 23.281436119620626 29.830296411201157 27.468405608379566 4 13.490636518702209 16.776293582606115 36.315575498178006 33.41749440051367 5 14.11724562752886 36.90510912737514 34.40262883178922 14.575016413306772 6 33.230494810067526 36.08236983163286 16.720111194622092 13.967024163677525 7 29.2418828075892 31.891006167311005 20.245199743766303 18.621911281333496 8 26.472593646064457 35.5142826281656 19.216439461789225 18.796684263980715 9 25.880690810841628 16.764892083308954 19.668711435553774 37.68570567029565 10-11 25.15026350965152 26.349652479301028 27.34508962928625 21.154994381761203 12-13 25.970140073419657 23.318335111067107 27.666338123463234 23.045186692050006 14-15 22.539545200193825 25.11598400189625 25.61200547872045 26.732465319189476 16-17 22.54970903673833 28.172467179434097 26.49839703921066 22.779426744616917 18-19 22.579506705131724 26.91220770531325 28.39468390093297 22.113601688622055 20-21 23.004268811348695 26.105514125107455 28.646173221778664 22.244043841765194 22-23 22.644634019373548 26.14346911618878 28.450397477268265 22.761499387169412 24-25 22.60796756566191 26.28589698872224 28.563651638406633 22.542483807209216 26-27 22.33716991011563 26.58814849600089 28.329142430193706 22.745539163689777 28-29 22.39903297283593 26.941742839183352 28.20999086379859 22.44923332418213 30-31 22.610223244356632 26.513999090880453 28.110284002346308 22.76549366241661 32-33 22.306152008989184 26.477975603791897 28.09880749318535 23.117064894033565 34-35 22.901314250873998 26.623271525764675 27.988459303083392 22.48695492027793 36-37 23.026647659145794 26.273584534148842 27.96684035220171 22.732927454503653 38-39 22.51117224669521 26.569093524316393 28.05044998415351 22.869284244834887 40-41 23.01023731156785 26.397557402852062 28.010033836819254 22.58217144876084 42-43 22.780008288624146 26.41003186057109 27.854465261851143 22.955494588953627 44-45 22.745203494259496 26.550978953732418 28.073116614834852 22.630700937173238 46-47 23.05559431065185 26.728189756953043 27.50047256214192 22.71574337025319 48-49 22.847235661489485 26.78502848124528 27.406091401019232 22.961644456245995 50-51 22.914031945200794 26.950200201326474 27.42604402478926 22.709723828683472 52-53 23.216659240690152 26.632420913350106 27.39208330895513 22.758836537004616 54-55 22.78990937308256 26.82947182554506 27.530251478069367 22.850367323303015 56-57 22.82908327445059 26.964695857505262 27.32399310509332 22.882227762950826 58-59 22.788784225125607 26.645791421571936 27.412579754237683 23.152844599064775 60-61 22.54875266097492 26.841679680878034 27.618013018711963 22.991554639435087 62-63 22.581269436930956 26.874458991357365 27.650473537270155 22.893798034441527 64-65 22.381311892513867 26.817526504735373 27.515249505309946 23.285912097440814 66-67 22.384037432964547 26.909106320225717 27.413585726807018 23.293270520002714 68-69 22.7762503748185 26.60197754145694 27.517331663597794 23.104440420126764 70-71 22.65673185092451 26.604673456440885 27.25283429724224 23.485760395392365 72-73 22.589070462765854 26.70887471702529 27.25132729953989 23.45072752066897 74-75 22.583688505038413 26.937842326265905 27.10577065884164 23.372698509854047 76-77 22.564298455246863 26.592609428139802 27.385219906417696 23.45787221019564 78-79 22.609904452435494 26.727833460100005 27.36806140007411 23.294200687390394 80-81 22.67427327105054 26.68090498389235 27.182840358634568 23.461981386422543 82-83 22.633599388516267 26.76953450313102 27.236604712557828 23.360261395794886 84-85 22.57442085854411 26.835973307208643 27.18527350659443 23.404332327652813 86-87 22.630011799308246 26.80796457057044 27.008414039451544 23.55360959066977 88-89 22.510785492926 26.867100749236865 26.848442024623516 23.773671733213618 90-91 22.421933192860962 26.794904588555728 27.01457707139862 23.76858514718469 92-93 22.392135455353692 26.56836048367482 27.20681184812411 23.832692212847377 94-95 22.206697258200926 26.43952421225549 27.309526443799776 24.044252085743807 96-97 22.41510803953996 26.16592072468543 27.259696011218477 24.159275224556133 98-99 22.131258057710788 26.299955462834905 27.092923278872977 24.475863200581326 100-101 22.293625361735067 26.002075522931268 27.341301631164498 24.36299748416917 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 213.0 1 161.0 2 136.5 3 196.0 4 188.0 5 171.5 6 332.0 7 731.0 8 1159.5 9 1364.5 10 1231.0 11 1060.5 12 1096.5 13 1244.5 14 1454.0 15 1620.5 16 1739.0 17 1944.0 18 2185.0 19 2470.5 20 2925.0 21 3561.0 22 4440.5 23 5627.5 24 7028.5 25 8904.5 26 11913.0 27 15456.5 28 19001.5 29 23016.0 30 27341.5 31 33081.0 32 41293.5 33 45548.5 34 47014.0 35 51344.0 36 57044.0 37 62657.0 38 67222.5 39 71358.0 40 76479.5 41 82090.5 42 85665.5 43 89568.5 44 94156.0 45 98669.0 46 101577.5 47 103459.5 48 104639.5 49 104420.0 50 103484.0 51 100965.5 52 99489.0 53 99188.5 54 97726.0 55 91177.0 56 82748.5 57 74953.5 58 68068.5 59 60398.0 60 49744.0 61 41268.0 62 34201.0 63 25454.5 64 17839.0 65 13133.5 66 9746.0 67 7421.0 68 5774.0 69 4503.5 70 3439.5 71 2353.0 72 1633.5 73 1330.5 74 1023.5 75 739.0 76 534.5 77 332.0 78 209.5 79 139.0 80 87.0 81 50.0 82 22.0 83 17.5 84 14.5 85 6.5 86 3.5 87 1.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.500986379708932E-4 2 0.05100670738202073 3 7.500986379708932E-4 4 0.0 5 0.08697393707272506 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.2502959139126795E-4 26-27 0.008401104745274003 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010220093942353419 36-37 1.1251479569563398E-4 38-39 0.0041630474407384575 40-41 0.005156928136049891 42-43 4.125542508839912E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1251479569563398E-4 68-69 9.563757634128888E-4 70-71 9.376232974636165E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.00136893001429688 82-83 5.438215125288976E-4 84-85 0.004800631283013716 86-87 0.0021752860501155903 88-89 1.1251479569563398E-4 90-91 0.002775364960492305 92-93 7.688511039201655E-4 94-95 3.750493189854466E-5 96-97 1.875246594927233E-5 98-99 1.312672616449063E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2666316.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.44472459403388 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3260760527506 41.953148929527245 2 14.442333486705044 16.30387072304519 3 5.102379431460312 8.640072053491215 4 2.2215287882005312 5.015743225107873 5 1.1570131373483865 3.2653643944654394 6 0.6790765095437521 2.2998171935684946 7 0.422677522822166 1.6700541457449942 8 0.29696687469062844 1.3409770764370796 9 0.20407341939333548 1.0366981159157633 >10 1.0577593334672208 10.478727969147986 >50 0.059999059809108364 2.3257815808943727 >100 0.025729224821615212 2.6794758647732486 >500 0.002392995811218514 0.9175515287932045 >1k 0.0019276910700769046 1.8629804153705594 >5k 6.647210586472085E-5 0.20973678371742746 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5590 0.20965256931286466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4601 0.17256019166520398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3194 0.11979075248395164 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.750493189854466E-5 2 0.0 0.0 0.0 0.0 3.750493189854466E-5 3 0.0 0.0 0.0 0.0 3.750493189854466E-5 4 0.0 0.0 0.0 7.500986379708932E-5 3.750493189854466E-5 5 0.0 0.0 0.0 7.500986379708932E-5 3.750493189854466E-5 6 1.1251479569563398E-4 0.0 0.0 7.500986379708932E-5 3.750493189854466E-5 7 1.1251479569563398E-4 0.0 0.0 7.500986379708932E-5 7.500986379708932E-5 8 1.5001972759417863E-4 0.0 0.0 7.500986379708932E-5 7.500986379708932E-5 9 1.5001972759417863E-4 0.0 0.0 1.5001972759417863E-4 7.500986379708932E-5 10-11 1.5001972759417863E-4 0.0 0.0 1.5001972759417863E-4 7.500986379708932E-5 12-13 1.5001972759417863E-4 0.0 0.0 1.875246594927233E-4 1.5001972759417863E-4 14-15 1.5001972759417863E-4 0.0 0.0 2.625345232898126E-4 2.0627712544199562E-4 16-17 1.5001972759417863E-4 0.0 0.0 2.625345232898126E-4 2.2502959139126795E-4 18-19 1.6877219354345096E-4 0.0 0.0 2.625345232898126E-4 2.2502959139126795E-4 20-21 1.875246594927233E-4 0.0 0.0 3.375443870869019E-4 2.2502959139126795E-4 22-23 1.875246594927233E-4 0.0 0.0 4.6881164873180826E-4 2.4378205734054028E-4 24-25 1.875246594927233E-4 0.0 0.0 7.313461720216208E-4 2.625345232898126E-4 26-27 1.875246594927233E-4 0.0 0.0 0.0011814053548041567 2.625345232898126E-4 28-29 1.875246594927233E-4 0.0 0.0 0.0034879586665646535 2.812869892390849E-4 30-31 1.875246594927233E-4 0.0 0.0 0.011288984501461943 3.187919211376296E-4 32-33 1.875246594927233E-4 0.0 0.0 0.02689103617125652 3.750493189854466E-4 34-35 1.875246594927233E-4 0.0 0.0 0.05059415313113674 4.1255425088399124E-4 36-37 2.2502959139126795E-4 0.0 0.0 0.09423114139509345 4.1255425088399124E-4 38-39 2.2502959139126795E-4 0.0 0.0 0.15830831754375702 4.3130671683326354E-4 40-41 2.2502959139126795E-4 0.0 0.0 0.22904261910441223 4.500591827825359E-4 42-43 2.2502959139126795E-4 0.0 0.0 0.3005270193030384 4.500591827825359E-4 44-45 2.2502959139126795E-4 0.0 0.0 0.39127020203156715 4.6881164873180826E-4 46-47 2.625345232898126E-4 0.0 0.0 0.5029786416913824 4.8756411468108056E-4 48-49 2.625345232898126E-4 0.0 0.0 0.6180062678242189 4.8756411468108056E-4 50-51 2.625345232898126E-4 0.0 0.0 0.7447354327094013 4.8756411468108056E-4 52-53 2.625345232898126E-4 0.0 0.0 0.8658576102757513 5.625739784781699E-4 54-55 2.625345232898126E-4 0.0 0.0 1.001212909497599 5.625739784781699E-4 56-57 2.625345232898126E-4 0.0 0.0 1.1611526915789425 6.000789103767145E-4 58-59 2.625345232898126E-4 0.0 0.0 1.3372195943766605 6.000789103767145E-4 60-61 2.625345232898126E-4 0.0 0.0 1.5298261721416366 6.000789103767145E-4 62-63 2.625345232898126E-4 0.0 0.0 1.7182321975339758 6.000789103767145E-4 64-65 2.625345232898126E-4 0.0 0.0 1.9153018621948785 6.000789103767145E-4 66-67 3.0003945518835727E-4 0.0 0.0 2.143125571012588 6.000789103767145E-4 68-69 3.375443870869019E-4 0.0 0.0 2.3971089698295325 6.000789103767145E-4 70-71 3.375443870869019E-4 0.0 0.0 2.6697135673341044 6.563363082245316E-4 72-73 3.5629685303617423E-4 0.0 0.0 2.947325073247132 7.688511039201655E-4 74-75 3.750493189854466E-4 0.0 0.0 3.2310311305936734 8.438609677172548E-4 76-77 3.750493189854466E-4 0.0 0.0 3.5473102212941 8.813658996157994E-4 78-79 3.750493189854466E-4 0.0 0.0 3.8954122467104426 9.751282293621611E-4 80-81 3.750493189854466E-4 0.0 0.0 4.261516639438086 0.0010688905591085228 82-83 3.750493189854466E-4 0.0 0.0 4.64489205330501 0.0011251479569563399 84-85 3.9380178493471894E-4 0.0 0.0 5.03711863109999 0.0011439004229056122 86-87 4.1255425088399124E-4 0.0 0.0 5.444684726041475 0.001200157820753429 88-89 4.1255425088399124E-4 0.0 0.0 5.893018681956677 0.0012376627526519738 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3710 0.0 49.35243 1 GTATCAA 6430 0.0 41.71477 1 TCAACGC 8040 0.0 33.46152 4 ATCAACG 8015 0.0 33.44791 3 CAACGCA 8275 0.0 32.511253 5 TATCAAC 8310 0.0 32.44918 2 AACGCAG 8690 0.0 30.825533 6 CGCAGAG 9895 0.0 26.783644 8 ACGCAGA 9935 0.0 26.77142 7 TACATGG 6940 0.0 23.093628 2 GCAGAGT 11420 0.0 22.707945 9 GTACATG 7230 0.0 22.627777 1 GTAAGGT 1460 0.0 22.463713 4 GAGTACT 6840 0.0 21.803429 12-13 TAAGGTG 1675 0.0 20.715399 5 TGGTATC 1815 0.0 20.700089 2 AGAGTAC 9820 0.0 20.652264 10-11 CCGATCG 230 9.041105E-8 20.65016 9 ACATGGG 7310 0.0 20.547697 3 GTACTTT 7270 0.0 20.252497 14-15 >>END_MODULE