FastQCFastQC Report
Thu 26 May 2016
SRR938401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659297
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56170.21122123628913958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43810.16474278728551192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31900.11995651482327849No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35650.045.844031
GTATCAA60450.040.9090351
ATCAACG77600.031.4765763
TATCAAC78450.031.3172572
TCAACGC78550.030.9748974
CAACGCA79750.030.3896435
AACGCAG84200.028.8345496
ACGCAGA96400.024.8859027
CGCAGAG95700.024.7700928
GTACATG73450.024.1323321
TACATGG71950.023.6449452
ACATGGG74900.021.8254263
GTGTAGC19150.021.1494121
GCAGAGT108850.020.9044119
TGGTATC18700.020.838112
GAGTACT64650.020.57272112-13
TAAGGTG15950.020.259765
AGAGTAC94300.019.62032310-11
GTAGCCC15750.019.3101433
TAGCCCT20200.019.2907264
Produced by FastQC (version 0.11.5)