FastQCFastQC Report
Thu 26 May 2016
SRR938401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659297
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55770.20971707936345585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46820.1760615681512821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32820.1234160757523511No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.052.434961
GTATCAA61300.043.990071
ATCAACG81100.033.003213
TCAACGC82600.032.4038734
TATCAAC83400.032.04812
CAACGCA84550.031.712765
AACGCAG87200.030.8284636
ACGCAGA100500.026.60697
CGCAGAG101900.026.1947388
GCAGAGT116350.022.2883649
TATACCG1503.0149813E-522.1858125
AGAGTAC100750.021.44955410-11
TACATGG70100.021.2352982
GTACATG74250.021.2013551
TGGTATC15950.020.2759082
ATACCGA1908.264798E-619.9980816
GAGTACT72950.019.43439112-13
ACATGGG72600.019.1866683
CAGAGTA109400.019.0372510-11
GTATAGC11300.018.9393961
Produced by FastQC (version 0.11.5)