FastQCFastQC Report
Thu 26 May 2016
SRR938400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657831
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53660.2018939503677999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41730.15700772547238706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.10945014938873088No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36100.051.928571
GTATCAA62450.040.864141
ATCAACG78750.032.2296033
TCAACGC79600.032.0048644
CAACGCA79600.031.8848465
TATCAAC79750.031.587082
AACGCAG84700.030.0698416
ACGCAGA98850.025.5183857
CGCAGAG98450.025.4290568
TAAGGTG16050.024.2825855
TGGTATC17650.023.9667742
GTAAGGT15000.022.497354
GTGTAGC17550.022.0155181
GCAGAGT110250.021.9309869
GGTAAGG15150.021.3334243
TACATGG69000.021.2161182
GTACATG73900.020.9132541
GAGTACT65000.020.86350312-13
GTGGTAT20900.020.7690241
TAGCCCT19050.020.458944
Produced by FastQC (version 0.11.5)