##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938400_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2657831 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06297541115293 31.0 31.0 34.0 28.0 34.0 2 31.241724172831155 31.0 31.0 34.0 28.0 34.0 3 31.302941383406242 31.0 31.0 34.0 27.0 34.0 4 34.96080789184865 37.0 35.0 37.0 32.0 37.0 5 34.948012119656966 37.0 35.0 37.0 32.0 37.0 6 34.91968902462196 37.0 35.0 37.0 32.0 37.0 7 34.83243140741454 37.0 35.0 37.0 32.0 37.0 8 34.781899977839075 37.0 35.0 37.0 32.0 37.0 9 36.273006823985426 39.0 35.0 39.0 32.0 39.0 10-11 36.11016125555011 39.0 35.0 39.0 31.0 39.0 12-13 35.93089571910328 38.0 35.0 39.0 30.0 39.0 14-15 37.09933080771501 39.0 36.0 41.0 31.0 41.0 16-17 37.01855968268863 39.0 36.0 41.0 31.0 41.0 18-19 36.998120836125395 39.0 36.0 41.0 31.0 41.0 20-21 36.923565117571435 39.0 36.0 41.0 30.5 41.0 22-23 36.767847918095626 39.0 36.0 41.0 30.0 41.0 24-25 36.63046691080058 39.0 36.0 41.0 30.0 41.0 26-27 36.41444696822334 39.0 36.0 41.0 29.5 41.0 28-29 36.1831920464469 39.0 35.0 40.0 29.0 41.0 30-31 35.91185575757074 38.5 35.0 40.0 27.5 41.0 32-33 35.621499260110966 38.0 34.5 40.0 27.0 41.0 34-35 35.32250207029717 38.0 34.0 40.0 26.5 41.0 36-37 35.11152609025931 38.0 34.0 40.0 25.0 41.0 38-39 34.87578141725339 38.0 34.0 40.0 25.0 41.0 40-41 34.598823627235895 38.0 33.0 40.0 24.0 41.0 42-43 34.199814623277405 38.0 33.0 40.0 23.5 41.0 44-45 33.75056107781119 38.0 33.0 40.0 22.0 41.0 46-47 33.29853290145235 37.5 32.0 40.0 18.5 41.0 48-49 32.82756202331902 37.0 31.0 40.0 16.0 41.0 50-51 32.10684652259681 36.5 30.5 39.0 14.5 40.5 52-53 32.102549597773525 36.5 31.0 39.0 13.5 40.0 54-55 32.08066577596544 36.5 31.0 39.0 8.5 40.0 56-57 31.653463482064886 36.0 30.0 39.0 7.5 40.0 58-59 31.19728229522494 36.0 29.5 39.0 4.0 40.0 60-61 30.64147231332617 35.0 28.5 39.0 2.0 40.0 62-63 29.90716001130245 34.5 28.0 38.0 2.0 40.0 64-65 29.24229682022672 34.0 26.5 38.0 2.0 40.0 66-67 28.53849247751268 34.0 26.0 37.5 2.0 39.5 68-69 27.691632199338486 33.5 24.0 36.5 2.0 39.0 70-71 26.844692533121933 32.5 22.0 36.0 2.0 39.0 72-73 25.941511518226704 32.0 19.0 35.5 2.0 38.5 74-75 24.99367604637014 31.0 15.0 35.0 2.0 37.5 76-77 24.04196617467401 30.5 7.5 34.5 2.0 37.0 78-79 23.04626629759379 29.5 2.0 34.0 2.0 36.0 80-81 22.08136202038429 29.0 2.0 34.0 2.0 35.5 82-83 21.073928327271375 28.0 2.0 34.0 2.0 35.0 84-85 20.073325956390754 26.0 2.0 33.0 2.0 35.0 86-87 19.071454317449078 25.5 2.0 32.5 2.0 35.0 88-89 17.973189604606162 23.0 2.0 32.0 2.0 34.0 90-91 16.809412261351454 19.0 2.0 31.5 2.0 34.0 92-93 15.636590513091313 6.0 2.0 31.0 2.0 34.0 94-95 14.378237555359991 2.0 2.0 30.5 2.0 34.0 96-97 12.949392568601992 2.0 2.0 29.5 2.0 34.0 98-99 11.258547477247426 2.0 2.0 28.0 2.0 34.0 100-101 7.955545330007815 2.0 2.0 10.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6527955720107492 1101 2 0.624648945626209 1101 3 0.5662826232565692 1101 4 0.43792850470202893 1101 5 0.4479041160390622 1101 6 0.66754903814914 1101 7 0.5264945627228457 1101 8 0.555590280825065 1101 9 0.7175949487600306 1101 10-11 0.8129423625615502 1101 12-13 0.7806042414260972 1101 14-15 0.8486018631883709 1101 16-17 0.9091097894031677 1101 18-19 0.7875134818940381 1101 20-21 0.9397480196475527 1101 22-23 0.950463672199156 1101 24-25 1.057341401139091 1101 26-27 1.1063483155668408 1101 28-29 1.1929266205180724 1101 30-31 1.2329191841294715 1101 32-33 1.1129462225342692 1101 34-35 1.2031807921498654 1101 36-37 1.3207750618166898 1101 38-39 1.450251385428146 1101 40-41 1.3517223074005429 1101 42-43 1.5150858156004219 1101 44-45 1.671458883328647 1101 46-47 1.7555458537539224 1101 48-49 1.3701192393482842 1101 50-51 1.490803508426012 1101 52-53 1.610789690467449 1101 54-55 1.712152603105288 1101 56-57 1.9865483886447777 1101 58-59 1.9774885286242778 1101 60-61 1.9461428385351134 1101 62-63 1.9690981222146533 1101 64-65 1.9960095396579334 1101 66-67 2.2959631101614058 1101 68-69 2.324970145269152 1101 70-71 2.7476376695974984 1101 72-73 2.8668056817866585 1101 74-75 2.811832022122804 1101 76-77 3.0154715152320506 1101 78-79 3.2144474552737456 1101 80-81 3.471776297407853 1101 82-83 3.5475302164538114 1101 84-85 3.688188653443653 1101 86-87 3.8902895767651593 1101 88-89 4.16400789505067 1101 90-91 3.7720158734941727 1101 92-93 3.856468252198889 1101 94-95 3.7082675487659404 1101 96-97 3.663690562496358 1101 98-99 3.2821858057407347 1101 100-101 2.27620490449537 1102 1 0.6070218293505114 1102 2 0.5558776340779126 1102 3 0.5651633983572388 1102 4 0.2876922591760547 1102 5 0.41819891028981004 1102 6 0.47991924828697563 1102 7 0.5236297341195666 1102 8 0.5955021946043715 1102 9 0.4524278710606282 1102 10-11 0.6133175395717814 1102 12-13 0.6018596696895528 1102 14-15 0.6857099530022026 1102 16-17 0.8836634907242242 1102 18-19 0.8258861400811526 1102 20-21 0.8042974594150749 1102 22-23 0.7217601734909351 1102 24-25 0.7510788380248528 1102 26-27 0.6576584483857602 1102 28-29 0.7059963424373379 1102 30-31 0.6059129421179961 1102 32-33 0.8338801742011768 1102 34-35 0.9203068688045875 1102 36-37 1.1094783760289815 1102 38-39 1.1182725093047665 1102 40-41 1.1909660888291214 1102 42-43 1.42382634052462 1102 44-45 1.397185343613934 1102 46-47 1.4528316615406212 1102 48-49 1.5883384543876673 1102 50-51 1.654243537872766 1102 52-53 1.5957295296716865 1102 54-55 1.6434047316136642 1102 56-57 1.954989906157067 1102 58-59 1.9593291386585605 1102 60-61 1.8032097585358677 1102 62-63 1.9555298832192278 1102 64-65 2.1846682723947097 1102 66-67 2.4418199969710344 1102 68-69 2.5273202151053944 1102 70-71 2.354509205383966 1102 72-73 2.550377090482648 1102 74-75 2.551979637013865 1102 76-77 2.5943654530587352 1102 78-79 3.2116103010599524 1102 80-81 3.2948411468198557 1102 82-83 3.284254297648957 1102 84-85 3.254757355796066 1102 86-87 3.415760827762494 1102 88-89 3.767366220021195 1102 90-91 3.5742594498600617 1102 92-93 3.6257024065693546 1102 94-95 3.4969097676047287 1102 96-97 3.7100937840977455 1102 98-99 3.5716277085773154 1102 100-101 2.906552942456769 1103 1 0.5573002155405362 1103 2 0.5414327045073932 1103 3 0.47920617444910363 1103 4 0.43131042566410827 1103 5 0.38756891193763465 1103 6 0.3934742922550356 1103 7 0.4791144051379348 1103 8 0.3873001159393752 1103 9 0.5500984840875844 1103 10-11 0.6114629847791946 1103 12-13 0.6131774906154774 1103 14-15 0.7125346184155603 1103 16-17 0.6698529436824217 1103 18-19 0.7826477250722377 1103 20-21 0.7293139075945945 1103 22-23 0.7003163480833976 1103 24-25 0.8011118245932707 1103 26-27 0.8157624393696281 1103 28-29 0.8571686435827601 1103 30-31 0.7393380484748633 1103 32-33 1.1002361897845532 1103 34-35 0.9575211004791555 1103 36-37 1.0041369575283667 1103 38-39 0.7444335042061283 1103 40-41 1.0400537410875543 1103 42-43 1.2450585346937189 1103 44-45 1.09702997296759 1103 46-47 1.3378298693806556 1103 48-49 1.2532968558290918 1103 50-51 1.6128129191205147 1103 52-53 1.4613229451075895 1103 54-55 1.4869813416408562 1103 56-57 1.6377369987082986 1103 58-59 1.7525893542710378 1103 60-61 1.8663446071464556 1103 62-63 1.997728183914802 1103 64-65 2.2065485609204387 1103 66-67 2.389025249803911 1103 68-69 2.416874899438671 1103 70-71 2.4358062119957147 1103 72-73 2.5318037279936405 1103 74-75 2.6559522783088383 1103 76-77 2.7549255451444843 1103 78-79 3.086660367273648 1103 80-81 2.970361715163097 1103 82-83 3.177775789736394 1103 84-85 3.429379889621586 1103 86-87 3.5005683329435193 1103 88-89 3.6435377393518635 1103 90-91 3.5318987722552073 1103 92-93 3.5450040896360893 1103 94-95 3.597407843739054 1103 96-97 3.764129962875929 1103 98-99 3.535731285800768 1103 100-101 2.3113276054044034 1104 1 0.4162503680577352 1104 2 0.37312146347581177 1104 3 0.37738072124266253 1104 4 0.27095662241229945 1104 5 0.3093507675306313 1104 6 0.36806864632686853 1104 7 0.46544899004862117 1104 8 0.39563007765902825 1104 9 0.6464123869043661 1104 10-11 0.5146988176307659 1104 12-13 0.609543521013542 1104 14-15 0.6416270057843363 1104 16-17 0.6876272176544589 1104 18-19 0.5875068145275151 1104 20-21 0.5777527144528065 1104 22-23 0.7176603297028805 1104 24-25 0.7165599857824674 1104 26-27 0.5969281042876418 1104 28-29 0.7182847178619127 1104 30-31 0.6598695436416477 1104 32-33 0.691241698042063 1104 34-35 0.6403429507119398 1104 36-37 0.7357473461644659 1104 38-39 0.8923257946785981 1104 40-41 0.7989828621801607 1104 42-43 1.008600037611636 1104 44-45 0.8430246963563945 1104 46-47 1.1274387421507086 1104 48-49 1.1466008167521622 1104 50-51 1.2550790529720075 1104 52-53 1.397344535461258 1104 54-55 1.6993653717313961 1104 56-57 1.7940662692113634 1104 58-59 1.8583572332010476 1104 60-61 1.883164449586868 1104 62-63 1.9751416651581764 1104 64-65 2.111127598846579 1104 66-67 2.2919678023745966 1104 68-69 2.524070237993069 1104 70-71 2.361138856403141 1104 72-73 2.6966598882253194 1104 74-75 2.7478766407731996 1104 76-77 2.862556688031326 1104 78-79 2.9369091794777695 1104 80-81 3.1844616174364084 1104 82-83 3.511456091466467 1104 84-85 3.645949230471828 1104 86-87 3.508974317035232 1104 88-89 3.3653738843676173 1104 90-91 3.231350265829956 1104 92-93 3.0251583134018585 1104 94-95 3.094943702389555 1104 96-97 3.1034836667689323 1104 98-99 2.79914050120121 1104 100-101 2.0552385856405904 1105 1 0.6736478922011067 1105 2 0.5662904560469393 1105 3 0.5307662336158252 1105 4 0.48144388037673025 1105 5 0.4678636431330787 1105 6 0.49666118056392605 1105 7 0.45279169192435376 1105 8 0.485667348531301 1105 9 0.501037895022975 1105 10-11 0.6403884484611595 1105 12-13 0.638103574832023 1105 14-15 0.7425559620774962 1105 16-17 0.7685403880289456 1105 18-19 0.7000285463486193 1105 20-21 0.7692098203060596 1105 22-23 0.7629048183008678 1105 24-25 0.89852282589856 1105 26-27 0.7874353483186809 1105 28-29 0.818721639338456 1105 30-31 0.836457676972401 1105 32-33 0.8607642522107852 1105 34-35 0.9018173250490307 1105 36-37 1.0613274303904845 1105 38-39 1.1865481974080367 1105 40-41 1.2849647324483655 1105 42-43 1.3170237824842062 1105 44-45 1.35894486199021 1105 46-47 1.5929021144940876 1105 48-49 1.6731071097652546 1105 50-51 1.7402638744426042 1105 52-53 1.780538122292377 1105 54-55 1.765882086225524 1105 56-57 1.8513790423127858 1105 58-59 1.9634919820531422 1105 60-61 2.0842008306480118 1105 62-63 1.9951730248118977 1105 64-65 2.131486891034605 1105 66-67 2.383483625969472 1105 68-69 2.5988868691643106 1105 70-71 2.5863269041531645 1105 72-73 2.765775240228457 1105 74-75 2.780094142060584 1105 76-77 2.9857861447786433 1105 78-79 3.3381170892485805 1105 80-81 3.583768103373391 1105 82-83 3.597678953383067 1105 84-85 3.7004664161224454 1105 86-87 3.6009143718928627 1105 88-89 3.732148742020957 1105 90-91 3.546425219700417 1105 92-93 3.663046871290314 1105 94-95 3.715451903967276 1105 96-97 3.485223251612597 1105 98-99 2.9762088360302563 1105 100-101 2.1678390702353765 1106 1 0.3419545960781143 1106 2 0.26676546797057554 1106 3 0.1255016792590773 1106 4 0.3124687545877336 1106 5 0.1667289640400469 1106 6 -0.07197224171329708 1106 7 0.18812615531771115 1106 8 -0.132707244883413 1106 9 0.21079508250551982 1106 10-11 0.2963917849311599 1106 12-13 0.5201432203884835 1106 14-15 0.547772607045296 1106 16-17 0.07106145849225953 1106 18-19 0.5121438479199583 1106 20-21 0.5161337099039187 1106 22-23 0.44089101803360364 1106 24-25 0.5243711765695025 1106 26-27 0.4998037854843389 1106 28-29 0.5626373617819667 1106 30-31 0.4429305200831237 1106 32-33 0.5299602606926754 1106 34-35 0.4736356206349228 1106 36-37 0.6155164673043529 1106 38-39 0.6460863820503988 1106 40-41 0.7969986095425199 1106 42-43 0.27488258728459414 1106 44-45 0.8203240938071943 1106 46-47 1.0414197969257444 1106 48-49 0.6783686832127103 1106 50-51 0.5848019355517806 1106 52-53 0.793271036042583 1106 54-55 0.8674078283913715 1106 56-57 1.3152074536546365 1106 58-59 1.4148123905934753 1106 60-61 1.5877947061953215 1106 62-63 1.5173111101980297 1106 64-65 1.6491502556057185 1106 66-67 1.8048248149387085 1106 68-69 1.9659871909950972 1106 70-71 1.960043501856095 1106 72-73 2.2493109531330546 1106 74-75 2.343208048244115 1106 76-77 2.434517324767558 1106 78-79 2.9339169343487193 1106 80-81 2.520848224844105 1106 82-83 2.7044986293179463 1106 84-85 2.780449583384218 1106 86-87 2.457171703318412 1106 88-89 2.544565625676338 1106 90-91 2.633905105276469 1106 92-93 2.7738989655536503 1106 94-95 2.547626039557292 1106 96-97 2.4416873399807155 1106 98-99 2.362542065166039 1106 100-101 1.7994506464757576 1107 1 0.27399527266409507 1107 2 0.202289933141941 1107 3 0.1865813298623813 1107 4 0.2651854619679952 1107 5 0.2113751258580976 1107 6 0.23633148173033192 1107 7 0.35470954416958733 1107 8 0.3964446168384015 1107 9 0.43044494555977764 1107 10-11 0.34994404638649 1107 12-13 0.3392985057537956 1107 14-15 0.36564402609925395 1107 16-17 0.4673828923511749 1107 18-19 0.4668707892183477 1107 20-21 0.5206469048110165 1107 22-23 0.5137429130665438 1107 24-25 0.5302330094216714 1107 26-27 0.5343432591685442 1107 28-29 0.7124356444083659 1107 30-31 0.7094681061368675 1107 32-33 0.6409348887184336 1107 34-35 0.6878423531835978 1107 36-37 0.7359523803993184 1107 38-39 0.7934441620489139 1107 40-41 0.7910881979650597 1107 42-43 1.0001814621087846 1107 44-45 0.9387543995293086 1107 46-47 0.9137433404892903 1107 48-49 0.8978728100910871 1107 50-51 1.1493468685594053 1107 52-53 1.2424414964763741 1107 54-55 1.2449478017424767 1107 56-57 1.3146152558605735 1107 58-59 1.4285347238527848 1107 60-61 1.5791176205487112 1107 62-63 1.4372695638215376 1107 64-65 1.6737033313330123 1107 66-67 1.8767606540089616 1107 68-69 1.9486746586092778 1107 70-71 2.119997051341624 1107 72-73 2.1045926172088585 1107 74-75 2.2315186189747394 1107 76-77 2.0046950766366827 1107 78-79 2.3921461704135645 1107 80-81 2.5846182115196505 1107 82-83 2.7105871628881566 1107 84-85 2.652891103852056 1107 86-87 2.654456846556254 1107 88-89 2.91986866439127 1107 90-91 2.804589926506761 1107 92-93 2.9798426882570315 1107 94-95 2.743030492144454 1107 96-97 2.8418904453178975 1107 98-99 2.430698016753869 1107 100-101 1.5607185974713902 1108 1 0.3130757248005871 1108 2 0.2981759721954589 1108 3 0.23887787508267166 1108 4 0.20274292725269305 1108 5 0.2594762440974847 1108 6 0.2907949201578006 1108 7 0.12492699354479697 1108 8 0.21148092973291455 1108 9 0.2108441734034514 1108 10-11 0.18115051850872277 1108 12-13 0.2690480714896566 1108 14-15 0.3507347825084324 1108 16-17 0.4021180096725985 1108 18-19 0.33001412368807337 1108 20-21 0.4008861153579204 1108 22-23 0.41569108768131713 1108 24-25 0.4729741570006425 1108 26-27 0.4987101706760697 1108 28-29 0.424550802256185 1108 30-31 0.5746135938483334 1108 32-33 0.6020093991474198 1108 34-35 0.648476880614183 1108 36-37 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2.372343521908334 2202 90-91 2.6415940344924387 2202 92-93 2.564739940500319 2202 94-95 2.617840502238421 2202 96-97 2.4758661863734943 2202 98-99 2.477390077872755 2202 100-101 1.4085641587855209 2203 1 0.3012155056561774 2203 2 0.3562083344631368 2203 3 0.3855717127879075 2203 4 0.40009856495815654 2203 5 0.4365531759642778 2203 6 0.4788761986560104 2203 7 0.45406267505482134 2203 8 0.5034910507167467 2203 9 0.5655313739161656 2203 10-11 0.5823525405265357 2203 12-13 0.5131658798834096 2203 14-15 0.677099266383486 2203 16-17 0.7095091959846158 2203 18-19 0.7567096437478114 2203 20-21 0.7307730922999056 2203 22-23 0.7299545274197712 2203 24-25 0.8185187489593702 2203 26-27 0.7319316933107629 2203 28-29 0.7821738887204646 2203 30-31 0.6467510036988955 2203 32-33 0.8963592977184192 2203 34-35 0.9652855149439148 2203 36-37 0.9298064233758652 2203 38-39 1.1925776498341207 2203 40-41 1.1982094890441815 2203 42-43 1.2028147978363322 2203 44-45 1.358893368012879 2203 46-47 1.2258930284017495 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0.22091907192468874 2316 96-97 0.31898366670212575 2316 98-99 0.18760436190692964 2316 100-101 0.41659852677737863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8981.0 3 3441.0 4 3641.0 5 4298.0 6 5251.0 7 6658.0 8 8570.0 9 11077.0 10 14156.0 11 17528.0 12 21305.0 13 24851.0 14 28745.0 15 32449.0 16 36609.0 17 41975.0 18 42457.0 19 43928.0 20 47944.0 21 53259.0 22 58945.0 23 65448.0 24 72739.0 25 80069.0 26 89026.0 27 98438.0 28 108331.0 29 119906.0 30 133016.0 31 149148.0 32 166651.0 33 187806.0 34 211192.0 35 231351.0 36 228256.0 37 160055.0 38 39791.0 39 540.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.121799140406665 18.416554895863353 14.507629938988439 23.95401602474154 2 15.481149501771164 19.4465813477696 40.41810876277563 24.654160387683607 3 19.38604217008047 23.09022680909043 29.389504904285506 28.134226116543587 4 13.008669309380029 16.098280027990526 36.688219172306994 34.20483149032245 5 14.211652235592833 36.816933564351 34.28872840139243 14.68268579866374 6 33.230096124670695 35.54783231978412 17.10054654107039 14.121525014474793 7 29.300365178076067 31.901590869569475 20.22146307606258 18.57658087629188 8 26.49812871532722 35.150717178366946 19.4052389948399 18.945915111465933 9 26.089838362912598 16.848814520839984 19.733771552113506 37.32757556413391 10-11 25.138502825762522 26.196578220832915 27.386993023575307 21.27792592982925 12-13 26.04278379780584 23.309975628624727 27.553861755533145 23.093378818036285 14-15 22.68795748109671 25.027238578332504 25.602137235766975 26.682666704803815 16-17 22.701901010756362 28.029258419896298 26.404382327682562 22.864458241664778 18-19 22.661433837967852 26.81546206039243 28.35601149611518 22.16709260552454 20-21 23.151251387204837 26.058712286235895 28.540917390999148 22.249118935560112 22-23 22.72775261155851 26.062816664819273 28.38378171776368 22.82564900585854 24-25 22.662041310886355 26.193636710449077 28.489470144943247 22.65485183372132 26-27 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27.287801140838503 22.764653799276694 52-53 23.19866630518485 26.68317166052794 27.323096072949088 22.795065961338125 54-55 22.821032954156774 26.801126278925995 27.487271415084507 22.890569351832724 56-57 22.84856849680782 26.906924353672178 27.327004042382747 22.917503107137254 58-59 22.84271427337826 26.568444650218108 27.408920539236536 23.179920537167096 60-61 22.621187013381856 26.753800757668117 27.603505833021025 23.021506395929002 62-63 22.7046893129557 26.77220109037688 27.63046319662889 22.892646400038533 64-65 22.499839142938523 26.764263317929938 27.493764201985293 23.242133337146242 66-67 22.453352266719946 26.802932868878397 27.39510915104907 23.34860571335259 68-69 22.936340599962037 26.50206394630748 27.495553875291773 23.06604157843871 70-71 22.78669925435898 26.51255247051209 27.217703470815014 23.48304480431392 72-73 22.826195549409455 26.5969774366066 27.17610923749183 23.400717776492115 74-75 22.82872510718745 26.848376703766778 27.07671212051655 23.246186068529223 76-77 22.93447202328051 26.53156481946243 27.146726799449443 23.387236357807616 78-79 22.927878265646694 26.69911461135841 27.062451318675944 23.31055580431895 80-81 23.10920298541104 26.660422758255947 26.796112997683746 23.434261258649265 82-83 23.103598276102773 26.671620005064973 26.92501920061472 23.299762518217534 84-85 23.116189008352592 26.67793113984026 26.877357844505866 23.328522007301274 86-87 23.171236443430388 26.70155078427182 26.679840707485127 23.44737206481267 88-89 23.152219132058498 26.661100985133157 26.541113320700475 23.64556656210787 90-91 23.106074220209088 26.67330044919163 26.540110454737135 23.680514875862148 92-93 23.275201287820604 26.35940068778591 26.68880641411726 23.676591610276223 94-95 23.12335943189067 26.19962103464692 26.74055926555235 23.936460267910064 96-97 23.300747454357566 25.9378930231823 26.681057289279366 24.080302233180774 98-99 23.14291783372728 26.095302889506307 26.271314476355652 24.490464800410763 100-101 24.269879474499323 25.123386723999126 26.42572365969864 24.181010141802904 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 184.0 1 137.0 2 123.0 3 173.0 4 179.0 5 159.0 6 247.5 7 623.0 8 1052.5 9 1215.0 10 1077.5 11 924.0 12 979.0 13 1145.5 14 1367.5 15 1527.5 16 1668.0 17 1889.5 18 2161.5 19 2471.5 20 2944.0 21 3615.0 22 4533.5 23 5662.0 24 7063.5 25 9068.0 26 11893.5 27 15396.0 28 18891.0 29 22674.5 30 26984.0 31 32791.5 32 39977.5 33 44024.5 34 46512.0 35 50945.5 36 56196.0 37 61675.5 38 66317.0 39 70540.0 40 75201.5 41 80424.5 42 84568.0 43 88123.5 44 92105.5 45 96378.5 46 99388.5 47 101426.0 48 103031.5 49 102895.5 50 101463.0 51 99169.0 52 97890.0 53 97259.5 54 95442.5 55 90129.0 56 82999.0 57 75840.5 58 69224.5 59 61667.0 60 51808.0 61 43315.0 62 36387.5 63 28441.0 64 21077.0 65 15943.5 66 11957.0 67 9145.0 68 7096.0 69 5499.0 70 4291.0 71 3088.5 72 2166.0 73 1686.5 74 1312.0 75 987.0 76 709.0 77 496.5 78 324.5 79 203.0 80 127.0 81 85.5 82 50.5 83 31.0 84 20.5 85 12.5 86 10.0 87 5.5 88 1.5 89 1.5 90 1.5 91 2.5 92 1.5 93 0.0 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3875340456184009 2 0.03081460032635634 3 0.03653354934907449 4 0.045826841510991485 5 0.06610653574286701 6 0.05602312562386397 7 0.039317774531187275 8 0.041387131085460285 9 0.0381890345924929 10-11 0.0386029059033475 12-13 0.030871037323291058 14-15 0.02836899712585187 16-17 0.023063919413988325 18-19 0.028575932781279168 20-21 0.036100865705908314 22-23 0.03538599707806854 24-25 0.044039669941392065 26-27 0.03865934290028222 28-29 0.03380576116389643 30-31 0.03632661369364719 32-33 0.030983911317160495 34-35 0.03215027592047801 36-37 0.03468994078254035 38-39 0.016404353775691532 40-41 0.013996375239810205 42-43 0.014711243867649975 44-45 0.011701270697798317 46-47 0.020844064201222725 48-49 0.017476656717451185 50-51 0.011738895362421464 52-53 0.009462603152721148 54-55 0.008653672863323515 56-57 0.009029919509554972 58-59 0.004157525440857602 60-61 0.007637806918498581 62-63 0.011644833700863598 64-65 0.007223935607643978 66-67 0.003461469145329406 68-69 0.006753627299854657 70-71 0.014523120544534246 72-73 0.011983455682471911 74-75 0.012679511978000107 76-77 0.005907072345833877 78-79 0.009067544174178117 80-81 0.011569584371617309 82-83 0.01369537792282504 84-85 0.008014053564730037 86-87 0.0029723485052285115 88-89 0.017852903363682643 90-91 0.027259069519469072 92-93 0.011964643350160337 94-95 0.022424300115394848 96-97 0.020505442219614416 98-99 0.012623074981065388 100-101 0.12687036910924734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2657831.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.24861178986996 #Duplication Level Percentage of deduplicated Percentage of total 1 76.79513509348442 45.50005146504478 2 13.175527581867858 15.61263437649626 3 4.527653531934917 8.047715592979365 4 1.9882632081074152 4.712073398129507 5 1.0285419305627181 3.046984077675694 6 0.6225086734337543 2.2129664836862064 7 0.3881834031948636 1.6099529431414132 8 0.27061728261194784 1.282695865688386 9 0.19535017899967064 1.0416804226769782 >10 0.9322614928738293 9.730909433403518 >50 0.04947743854814833 1.992340898002875 >100 0.022779726018896895 2.510497197495978 >500 0.001914030187073774 0.792199968500023 >1k 0.0017226271683265996 1.7044565815262571 >5k 6.38010062343185E-5 0.20284129555267014 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5366 0.2018939503677999 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4173 0.15700772547238706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2909 0.10945014938873088 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.7624664623145714E-5 2 0.0 0.0 0.0 0.0 3.7624664623145714E-5 3 0.0 0.0 0.0 0.0 3.7624664623145714E-5 4 0.0 0.0 0.0 0.0 3.7624664623145714E-5 5 0.0 0.0 0.0 0.0 3.7624664623145714E-5 6 0.0 0.0 0.0 3.7624664623145714E-5 3.7624664623145714E-5 7 0.0 0.0 0.0 3.7624664623145714E-5 3.7624664623145714E-5 8 0.0 0.0 0.0 3.7624664623145714E-5 3.7624664623145714E-5 9 0.0 0.0 0.0 3.7624664623145714E-5 3.7624664623145714E-5 10-11 0.0 3.7624664623145714E-5 0.0 3.7624664623145714E-5 3.7624664623145714E-5 12-13 0.0 3.7624664623145714E-5 0.0 1.1287399386943715E-4 1.5049865849258286E-4 14-15 0.0 3.7624664623145714E-5 0.0 1.5049865849258286E-4 1.5049865849258286E-4 16-17 0.0 3.7624664623145714E-5 0.0 1.8812332311572856E-4 2.257479877388743E-4 18-19 0.0 3.7624664623145714E-5 0.0 5.267453047240401E-4 2.257479877388743E-4 20-21 0.0 3.7624664623145714E-5 0.0 5.643699693471857E-4 2.257479877388743E-4 22-23 3.7624664623145714E-5 3.7624664623145714E-5 0.0 7.713056247744872E-4 2.6337265236202003E-4 24-25 7.524932924629143E-5 3.7624664623145714E-5 0.0 0.00105349060944808 3.009973169851657E-4 26-27 7.524932924629143E-5 3.7624664623145714E-5 0.0 0.0014485495879911102 3.009973169851657E-4 28-29 9.406166155786428E-5 3.7624664623145714E-5 0.0 0.00355553080688727 3.009973169851657E-4 30-31 1.1287399386943715E-4 3.7624664623145714E-5 0.0 0.010516093762169228 3.009973169851657E-4 32-33 1.1287399386943715E-4 3.7624664623145714E-5 0.0 0.02573527060223167 3.009973169851657E-4 34-35 1.3168632618101E-4 3.7624664623145714E-5 0.0 0.05019130260727639 3.1980964929673855E-4 36-37 1.5049865849258286E-4 3.7624664623145714E-5 0.0 0.09255667497293846 3.3862198160831145E-4 38-39 1.8812332311572856E-4 3.7624664623145714E-5 0.0 0.1542611249548974 3.3862198160831145E-4 40-41 2.257479877388743E-4 3.7624664623145714E-5 0.0 0.22253107891359533 3.3862198160831145E-4 42-43 2.821849846735929E-4 3.7624664623145714E-5 0.0 0.2946387486638541 3.574343139198843E-4 44-45 3.009973169851657E-4 3.7624664623145714E-5 0.0 0.38412901347000616 4.326836431661757E-4 46-47 3.7624664623145713E-4 3.7624664623145714E-5 0.0 0.49152861863677566 5.079329724124672E-4 48-49 3.7624664623145713E-4 3.7624664623145714E-5 0.0 0.6078452693192307 5.45557637035613E-4 50-51 3.7624664623145713E-4 3.7624664623145714E-5 0.0 0.7332294641758637 6.396192985934771E-4 52-53 3.7624664623145713E-4 3.7624664623145714E-5 0.0 0.8540610746130961 7.148686278397686E-4 54-55 3.7624664623145713E-4 3.7624664623145714E-5 0.0 0.9892088699394357 7.148686278397686E-4 56-57 3.7624664623145713E-4 3.7624664623145714E-5 0.0 1.146592842058054 7.336809601513415E-4 58-59 3.7624664623145713E-4 3.7624664623145714E-5 0.0 1.3180860634103522 7.524932924629143E-4 60-61 3.7624664623145713E-4 3.7624664623145714E-5 0.0 1.5012993677927602 7.524932924629143E-4 62-63 3.7624664623145713E-4 3.7624664623145714E-5 0.0 1.6859988464277826 7.9011795708606E-4 64-65 3.7624664623145713E-4 3.7624664623145714E-5 0.0 1.885221445607339 7.9011795708606E-4 66-67 3.7624664623145713E-4 7.524932924629143E-5 0.0 2.1094644467612875 7.9011795708606E-4 68-69 3.7624664623145713E-4 7.524932924629143E-5 0.0 2.3645596729062155 7.9011795708606E-4 70-71 3.9505897854303E-4 7.524932924629143E-5 0.0 2.6287412555576335 7.9011795708606E-4 72-73 4.1387131085460286E-4 7.524932924629143E-5 0.0 2.9043231115898642 8.277426217092057E-4 74-75 4.1387131085460286E-4 7.524932924629143E-5 0.0 3.1880695198453175 8.465549540207786E-4 76-77 4.1387131085460286E-4 7.524932924629143E-5 0.0 3.4898945794521925 8.841796186439243E-4 78-79 4.1387131085460286E-4 7.524932924629143E-5 0.0 3.8218381830898958 9.029919509554972E-4 80-81 5.079329724124672E-4 7.524932924629143E-5 0.0 4.171860438079021 9.2180428326707E-4 82-83 5.267453047240401E-4 7.524932924629143E-5 0.0 4.5415603926660495 9.782412802017886E-4 84-85 5.267453047240401E-4 7.524932924629143E-5 0.0 4.91329207914273 9.782412802017886E-4 86-87 5.267453047240401E-4 7.524932924629143E-5 0.0 5.296499288329469 9.970536125133616E-4 88-89 5.267453047240401E-4 7.524932924629143E-5 0.0 5.696487097938131 0.0010158659448249343 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3610 0.0 51.92857 1 GTATCAA 6245 0.0 40.86414 1 ATCAACG 7875 0.0 32.229603 3 TCAACGC 7960 0.0 32.004864 4 CAACGCA 7960 0.0 31.884846 5 TATCAAC 7975 0.0 31.58708 2 AACGCAG 8470 0.0 30.069841 6 ACGCAGA 9885 0.0 25.518385 7 CGCAGAG 9845 0.0 25.429056 8 TAAGGTG 1605 0.0 24.282585 5 TGGTATC 1765 0.0 23.966774 2 GTAAGGT 1500 0.0 22.49735 4 GTGTAGC 1755 0.0 22.015518 1 GCAGAGT 11025 0.0 21.930986 9 GGTAAGG 1515 0.0 21.333424 3 TACATGG 6900 0.0 21.216118 2 GTACATG 7390 0.0 20.913254 1 GAGTACT 6500 0.0 20.863503 12-13 GTGGTAT 2090 0.0 20.769024 1 TAGCCCT 1905 0.0 20.45894 4 >>END_MODULE