FastQCFastQC Report
Thu 26 May 2016
SRR938400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657831
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54120.20362468494046462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45410.1708536020537047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.1176899509411998No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34950.051.928411
GTATCAA58450.042.1050381
ATCAACG76400.031.7688853
TCAACGC77700.031.2373584
TATCAAC78750.031.063292
CAACGCA79450.030.6090955
AACGCAG82900.029.3919966
ACGCAGA93700.025.7000897
TATACCG1301.0030963E-525.5757965
CGCAGAG95500.025.0167488
TAAGGTG14950.022.5575315
GTATAGC11600.022.1169171
TACATGG67350.021.7222022
GCAGAGT109350.021.4138329
GTACATG73000.020.8264851
GAGTACT68050.020.65997712-13
GTGGTAT19350.020.6246781
GTAAGGT15350.020.422554
CAGAGTA101350.020.03450610-11
AGAGTAC97550.019.9872110-11
Produced by FastQC (version 0.11.5)