##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938400_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2657831 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.835211493883545 31.0 31.0 34.0 30.0 34.0 2 32.095370247393454 33.0 31.0 34.0 30.0 34.0 3 32.157429874209456 34.0 31.0 34.0 30.0 34.0 4 35.797366724972356 37.0 35.0 37.0 35.0 37.0 5 35.68945881058653 37.0 35.0 37.0 33.0 37.0 6 35.71956418598474 37.0 35.0 37.0 33.0 37.0 7 35.64361428548316 37.0 35.0 37.0 33.0 37.0 8 35.611963288862235 37.0 35.0 37.0 33.0 37.0 9 37.16876844314029 39.0 37.0 39.0 33.0 39.0 10-11 37.10405044564534 39.0 37.0 39.0 33.0 39.0 12-13 36.985907493742076 39.0 37.0 39.0 33.0 39.0 14-15 38.01955974627431 40.0 37.0 41.0 33.0 41.0 16-17 38.10540700292833 40.0 37.0 41.0 33.0 41.0 18-19 38.232901753346994 40.0 38.0 41.0 33.0 41.0 20-21 38.18027538244531 40.0 37.5 41.0 33.0 41.0 22-23 38.05385556869493 40.0 37.0 41.0 33.0 41.0 24-25 37.93411958849152 40.0 37.0 41.0 33.0 41.0 26-27 37.72015526946596 40.0 37.0 41.0 32.0 41.0 28-29 37.581788495957795 39.5 37.0 41.0 32.0 41.0 30-31 37.3376168386929 39.0 36.0 41.0 31.5 41.0 32-33 37.24688383121425 39.0 36.0 41.0 31.0 41.0 34-35 37.116302165186575 39.0 36.0 41.0 31.0 41.0 36-37 36.993921171060165 39.0 36.0 41.0 31.0 41.0 38-39 36.77877242759227 39.0 35.5 41.0 30.0 41.0 40-41 36.5568510563689 39.0 35.0 40.0 30.0 41.0 42-43 36.31056865541865 39.0 35.0 40.0 30.0 41.0 44-45 36.03357098325665 38.0 35.0 40.0 29.5 41.0 46-47 35.64760701489297 38.0 34.5 40.0 28.0 41.0 48-49 35.25446971609557 38.0 34.0 40.0 27.0 41.0 50-51 34.86439788684834 38.0 33.5 40.0 26.0 41.0 52-53 34.253635012910905 37.5 33.0 40.0 24.0 41.0 54-55 33.81662998888943 37.0 32.5 40.0 23.0 41.0 56-57 33.418231821361104 37.0 32.0 39.0 23.0 40.5 58-59 32.864529196927876 36.0 31.0 39.0 21.0 40.0 60-61 32.35314980523592 36.0 31.0 39.0 19.0 40.0 62-63 31.817300460413023 35.0 30.0 38.5 17.0 40.0 64-65 31.152795456144503 35.0 29.0 38.0 12.0 40.0 66-67 30.475163018265647 34.0 28.5 38.0 8.0 40.0 68-69 29.72201148229515 34.0 28.0 37.0 6.5 39.0 70-71 28.92592587715321 33.5 26.0 36.0 2.0 39.0 72-73 28.326902274824846 33.0 26.0 36.0 2.0 38.5 74-75 27.602657768684317 32.0 25.5 35.0 2.0 38.0 76-77 26.44384180183014 30.5 24.0 34.0 2.0 36.0 78-79 26.933303697639165 32.0 25.0 35.0 2.0 36.0 80-81 26.548549550366445 32.0 24.5 35.0 2.0 36.0 82-83 25.857111306174094 31.5 22.0 34.5 2.0 36.0 84-85 25.058350963624097 31.0 19.5 34.0 2.0 35.0 86-87 24.130923486105775 30.5 15.0 34.0 2.0 35.0 88-89 23.13677205209812 29.5 6.5 34.0 2.0 35.0 90-91 22.249990311648858 29.0 2.0 34.0 2.0 35.0 92-93 21.149021326036156 29.0 2.0 33.5 2.0 35.0 94-95 19.950724293606328 26.5 2.0 33.0 2.0 34.5 96-97 18.55766092727491 25.0 2.0 32.5 2.0 34.0 98-99 16.764176127075046 12.0 2.0 32.0 2.0 34.0 100-101 12.53557938785423 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27112557687916805 1101 2 0.35524365305001737 1101 3 0.3028175142018483 1101 4 0.2023666139966096 1101 5 0.14141786779222798 1101 6 0.22509616275173983 1101 7 0.23901767484910863 1101 8 0.2830273752791541 1101 9 0.22008485562218993 1101 10-11 0.28158766294325943 1101 12-13 0.2927011750930433 1101 14-15 0.5063194574503527 1101 16-17 0.3615250892352222 1101 18-19 0.4041712340407173 1101 20-21 0.41603343010741867 1101 22-23 0.4899307235172117 1101 24-25 0.3308288012122773 1101 26-27 0.49357776474091253 1101 28-29 0.5526352905291887 1101 30-31 0.47781471039810697 1101 32-33 0.3812335276321335 1101 34-35 0.26770053850236764 1101 36-37 0.30841968344129356 1101 38-39 0.5412734860088833 1101 40-41 0.5685828064887914 1101 42-43 0.5887892800001353 1101 44-45 0.579840521409956 1101 46-47 0.5574963808487681 1101 48-49 0.5967728747521406 1101 50-51 0.7912733687531954 1101 52-53 0.9185267269803887 1101 54-55 0.9850449105269519 1101 56-57 0.9769322917038679 1101 58-59 1.0920641523543608 1101 60-61 1.2112658973424075 1101 62-63 1.440430815930224 1101 64-65 1.4580805248645454 1101 66-67 1.5481982317257774 1101 68-69 1.8132535172107431 1101 70-71 1.8448883648191838 1101 72-73 2.207406941276435 1101 74-75 2.072400865151238 1101 76-77 2.0707736350924364 1101 78-79 2.0913869821266644 1101 80-81 2.1886562074684868 1101 82-83 2.3588995940406043 1101 84-85 2.561816293434113 1101 86-87 2.586947958326519 1101 88-89 2.4872322724970637 1101 90-91 2.9239511676269103 1101 92-93 3.034757226831804 1101 94-95 2.8574395840957685 1101 96-97 2.868839021832084 1101 98-99 2.8840029591475442 1101 100-101 2.3392736163721555 1102 1 0.2283137500246717 1102 2 0.27030656616211246 1102 3 0.26997829726306577 1102 4 0.2540759839097362 1102 5 0.20524680558563801 1102 6 0.2884895691696556 1102 7 0.34364773631347134 1102 8 0.2912279169108132 1102 9 0.3008012200482284 1102 10-11 0.37552217777643193 1102 12-13 0.4508321073177015 1102 14-15 0.5271064777684984 1102 16-17 0.3382076013801978 1102 18-19 0.43094303011766755 1102 20-21 0.31626544130057255 1102 22-23 0.49729755526237085 1102 24-25 0.33266536745871633 1102 26-27 0.32630528602901876 1102 28-29 0.2757560482570014 1102 30-31 0.40732123520253083 1102 32-33 0.4253110419335968 1102 34-35 0.4086817422989881 1102 36-37 0.43601631242844263 1102 38-39 0.5897443474475992 1102 40-41 0.6437623270776243 1102 42-43 0.6189539108600783 1102 44-45 0.5982569202550749 1102 46-47 0.6073666136705356 1102 48-49 0.8186341453060564 1102 50-51 0.8887958001312981 1102 52-53 0.9044510485646668 1102 54-55 0.8014065842498326 1102 56-57 0.9664663064018484 1102 58-59 1.0487206142789347 1102 60-61 1.090318456300956 1102 62-63 1.2043278962367658 1102 64-65 1.2366110057093316 1102 66-67 1.3845029325005953 1102 68-69 1.6549951769361293 1102 70-71 1.8465315127915751 1102 72-73 1.7530814795678076 1102 74-75 1.9189586247778436 1102 76-77 1.7574020808563802 1102 78-79 1.8715068500396725 1102 80-81 2.1831234467379304 1102 82-83 2.1906569238219404 1102 84-85 2.3179888920986045 1102 86-87 2.3213064841254045 1102 88-89 2.2338869227321005 1102 90-91 2.3873461921648698 1102 92-93 2.74114995031233 1102 94-95 2.547450883715346 1102 96-97 2.59087889049518 1102 98-99 2.4811546595648686 1102 100-101 2.303170182509712 1103 1 0.22909917314882122 1103 2 0.20332067851971658 1103 3 0.26069184154204805 1103 4 0.1269402765057137 1103 5 0.15399343626477702 1103 6 0.19134427551401956 1103 7 0.34792245879930306 1103 8 0.24411666226652784 1103 9 0.3291480186371132 1103 10-11 0.37381255941027547 1103 12-13 0.4145849203473091 1103 14-15 0.5051451511371212 1103 16-17 0.4336098991938826 1103 18-19 0.36834597984457673 1103 20-21 0.37927820653122524 1103 22-23 0.4277545319967615 1103 24-25 0.30235035024075074 1103 26-27 0.48473312873750984 1103 28-29 0.5151251718667211 1103 30-31 0.4673204052761051 1103 32-33 0.4978269157799673 1103 34-35 0.5357031844705631 1103 36-37 0.5526880308211801 1103 38-39 0.541662439723396 1103 40-41 0.7145324988002244 1103 42-43 0.7418254202075758 1103 44-45 0.5473597276916919 1103 46-47 0.6518481141293933 1103 48-49 0.7806119381588204 1103 50-51 0.7666853573715642 1103 52-53 1.0818911224641994 1103 54-55 1.0622813267020064 1103 56-57 1.1146825960533846 1103 58-59 1.1362661249182295 1103 60-61 1.0443469973504378 1103 62-63 1.2707283249118113 1103 64-65 1.45351979438961 1103 66-67 1.5012668148242483 1103 68-69 1.3516886418067848 1103 70-71 1.5764787162402207 1103 72-73 1.538661267371257 1103 74-75 1.4206987639889803 1103 76-77 1.4221369133495614 1103 78-79 1.4779007114708271 1103 80-81 1.7783878745277164 1103 82-83 1.941149316764438 1103 84-85 1.9458607081580404 1103 86-87 1.9489169936400863 1103 88-89 2.0316779051605103 1103 90-91 2.317330922721439 1103 92-93 2.675759265454637 1103 94-95 2.5541278823646074 1103 96-97 2.6183742627532673 1103 98-99 2.6901176544686365 1103 100-101 2.27455185364475 1104 1 0.09616725512012536 1104 2 0.12993288968839067 1104 3 0.1968861644604587 1104 4 0.1825757677004347 1104 5 0.1454325440627784 1104 6 0.12225253812936643 1104 7 0.23979788633566557 1104 8 0.16786006686045596 1104 9 0.21242976842872707 1104 10-11 0.20252227220606045 1104 12-13 0.23204160405959584 1104 14-15 0.380754153331317 1104 16-17 0.3454439195919292 1104 18-19 0.33268576061953326 1104 20-21 0.38847891790592826 1104 22-23 0.3720758039727414 1104 24-25 0.32844978979408523 1104 26-27 0.36206129598558334 1104 28-29 0.35628451447221465 1104 30-31 0.3150237565312466 1104 32-33 0.4340871677046465 1104 34-35 0.47483689594002954 1104 36-37 0.42956328512388353 1104 38-39 0.4989622052423357 1104 40-41 0.586733307815706 1104 42-43 0.6079843834170404 1104 44-45 0.5506687768164653 1104 46-47 0.6867488970596156 1104 48-49 0.8239237160715547 1104 50-51 0.8329616899607046 1104 52-53 0.9089584800038608 1104 54-55 0.9717865713866729 1104 56-57 1.080261856463082 1104 58-59 1.135808916923601 1104 60-61 0.9932035550173381 1104 62-63 1.14169989082664 1104 64-65 1.3603210192231252 1104 66-67 1.2115968807704327 1104 68-69 1.3316694171662604 1104 70-71 1.1701023433635136 1104 72-73 1.5525526736362458 1104 74-75 1.564460290827455 1104 76-77 1.5099025227755334 1104 78-79 1.7907128004883006 1104 80-81 1.8911982741591444 1104 82-83 2.0498958123621556 1104 84-85 2.169506071094002 1104 86-87 2.1497456947627853 1104 88-89 2.1499308480566874 1104 90-91 2.4383015580422622 1104 92-93 2.4092374278493054 1104 94-95 2.3610227535890083 1104 96-97 2.545162785694412 1104 98-99 2.5247443703050294 1104 100-101 2.189497923574452 1105 1 0.12053232958434279 1105 2 0.17993641248085623 1105 3 0.11514949034106792 1105 4 0.08314229801644046 1105 5 0.10040840001293816 1105 6 0.018712634955839746 1105 7 0.11204714988161868 1105 8 0.1815801873916456 1105 9 0.3075117053171539 1105 10-11 0.3003368895369505 1105 12-13 0.24627443371068125 1105 14-15 0.3930191837538288 1105 16-17 0.26385448187584615 1105 18-19 0.24727042066734128 1105 20-21 0.2286369022266399 1105 22-23 0.22507810725334565 1105 24-25 0.22438901659146637 1105 26-27 0.2481894097464803 1105 28-29 0.2546393023824223 1105 30-31 0.2736663089807294 1105 32-33 0.47398605676007577 1105 34-35 0.6428482537493778 1105 36-37 0.5150537734482086 1105 38-39 0.46879934885883046 1105 40-41 0.5471356243169794 1105 42-43 0.593968565942852 1105 44-45 0.6556721350305921 1105 46-47 0.7155426929188238 1105 48-49 0.639647734252982 1105 50-51 0.7244040517713515 1105 52-53 0.8764255595762549 1105 54-55 0.9231477566335968 1105 56-57 0.9674923050328985 1105 58-59 1.012190320823045 1105 60-61 1.0067840559937977 1105 62-63 1.0935303283520277 1105 64-65 1.4051561702176336 1105 66-67 1.4630877428643956 1105 68-69 1.6247494286241775 1105 70-71 1.6145991158599422 1105 72-73 1.7370585890712746 1105 74-75 1.5956669274964312 1105 76-77 1.5479091010124009 1105 78-79 1.7909510310290884 1105 80-81 1.8318342565326695 1105 82-83 2.0347841207988218 1105 84-85 2.4476592355817495 1105 86-87 2.352851478371928 1105 88-89 2.4970632133792385 1105 90-91 2.372023346119228 1105 92-93 2.471992643282345 1105 94-95 2.473555842675399 1105 96-97 2.5180189896583833 1105 98-99 2.6024086147231706 1105 100-101 2.545165307766391 1106 1 0.04982328136987846 1106 2 0.06591582648243133 1106 3 0.11776382835362398 1106 4 0.17181924694030926 1106 5 0.12852481982808683 1106 6 0.03317138776487383 1106 7 0.07852991324361369 1106 8 0.03198419459309321 1106 9 -0.03890244879209348 1106 10-11 0.18068466765847546 1106 12-13 0.13868254113943124 1106 14-15 0.16571115431211325 1106 16-17 0.13912907464604274 1106 18-19 0.2012892234912087 1106 20-21 0.18246651130321823 1106 22-23 0.20953454515804992 1106 24-25 0.18313215781459746 1106 26-27 0.08875520781378299 1106 28-29 0.27869054072196064 1106 30-31 0.43942984069759916 1106 32-33 0.12232107121883473 1106 34-35 0.0285204461088 1106 36-37 0.1316862805057113 1106 38-39 0.14966682568830691 1106 40-41 0.21722496565956817 1106 42-43 0.3215254073176581 1106 44-45 0.46125548555115614 1106 46-47 0.04157145573941534 1106 48-49 0.3202692189284164 1106 50-51 0.6085502065594923 1106 52-53 0.47556751423636 1106 54-55 0.3728618310370919 1106 56-57 0.7016929486944576 1106 58-59 0.8738146317227589 1106 60-61 0.8358364276159662 1106 62-63 0.6073324754660767 1106 64-65 0.8859918775363802 1106 66-67 0.8051386245690253 1106 68-69 0.8380435589720889 1106 70-71 0.7280459439576177 1106 72-73 1.3036644559304378 1106 74-75 1.3515244142515286 1106 76-77 1.3722647949251154 1106 78-79 1.563524973692509 1106 80-81 1.6901207546785955 1106 82-83 1.9006304465998518 1106 84-85 1.9101450292663031 1106 86-87 1.7962210498944096 1106 88-89 1.8738552501083383 1106 90-91 1.9371384897829422 1106 92-93 1.9994662877474134 1106 94-95 1.745413024357255 1106 96-97 1.7708051999182963 1106 98-99 1.6110638594048012 1106 100-101 1.4114317806470975 1107 1 0.06557666800101458 1107 2 0.08271018579844025 1107 3 0.12410682191347178 1107 4 0.05540611495422354 1107 5 0.058742637579221935 1107 6 -0.03507940800091092 1107 7 0.012444340662369768 1107 8 0.10161370084818344 1107 9 0.2068920215610035 1107 10-11 0.12078709937066634 1107 12-13 0.09582453906674004 1107 14-15 0.2676524527057751 1107 16-17 0.16197726844277582 1107 18-19 0.09526731217282247 1107 20-21 0.25176059302650344 1107 22-23 0.2672391309897151 1107 24-25 0.18334497889683377 1107 26-27 0.23817180045572428 1107 28-29 0.17133387109431197 1107 30-31 0.22520691524049852 1107 32-33 0.27532323644201995 1107 34-35 0.34234826460017587 1107 36-37 0.2260492977534696 1107 38-39 0.19250632157993408 1107 40-41 0.26821412103745956 1107 42-43 0.34240269283102265 1107 44-45 0.34660738058409635 1107 46-47 0.3613582645335498 1107 48-49 0.3617947759644693 1107 50-51 0.43296124734711583 1107 52-53 0.5249535716795464 1107 54-55 0.5164258439173963 1107 56-57 0.523080542150673 1107 58-59 0.6996853475418376 1107 60-61 0.7706992968142785 1107 62-63 0.816281139319802 1107 64-65 0.861684008708199 1107 66-67 0.8011389761864649 1107 68-69 1.0943564799877095 1107 70-71 1.0858328822950227 1107 72-73 1.3700831397708377 1107 74-75 1.3227912548762966 1107 76-77 1.4158098389698281 1107 78-79 1.5630276200763973 1107 80-81 1.8311100951183015 1107 82-83 1.8442721854406265 1107 84-85 2.0618610149055066 1107 86-87 2.311541854543165 1107 88-89 2.157605897824677 1107 90-91 2.366847387554799 1107 92-93 2.472130345042551 1107 94-95 2.123640039680371 1107 96-97 2.024825667754552 1107 98-99 2.1435491469908534 1107 100-101 1.7133092327160888 1108 1 0.07271306309947434 1108 2 0.06354795770239718 1108 3 0.049165818375897175 1108 4 0.06455333381892103 1108 5 0.04816570456879532 1108 6 0.017603436757589463 1108 7 -0.02252370015329319 1108 8 0.0974507422143347 1108 9 -0.027373374707792664 1108 10-11 -0.017056345639026915 1108 12-13 0.020673967107285307 1108 14-15 0.12793340721614754 1108 16-17 0.15372422047669687 1108 18-19 0.015165686246554344 1108 20-21 0.00619049312730624 1108 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88855.0 29 104931.0 30 122091.0 31 142115.0 32 165801.0 33 198467.0 34 239166.0 35 290571.0 36 332725.0 37 291671.0 38 93557.0 39 1890.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.01923713810877 19.15821902548292 15.094281396021003 23.7282624403873 2 15.998836505724151 20.174960639876698 39.26578694658763 24.560415907811517 3 19.42378290477439 23.305138154619847 29.839606920504796 27.431472020100966 4 13.52395995080199 16.840122641356807 36.30475376350114 33.331163644340066 5 14.113161744344247 36.926560976197074 34.406792925340426 14.553484354118257 6 33.18450270163905 36.03618890742112 16.72894175739541 14.05036663354442 7 29.233047548922407 31.973327122755357 20.223896854239413 18.569728474082815 8 26.415619665524005 35.567148977605406 19.20179966431249 18.8154316925581 9 25.883815438424605 16.77323884999114 19.67203986119467 37.67090585038959 10-11 25.106054523406492 26.35594588218739 27.356686711833824 21.181312882572293 12-13 25.962373077896977 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27.98447213440427 22.722004362626645 38-39 22.519393044279923 26.575817725138997 27.999924742006186 22.904864488574898 40-41 23.038806472242022 26.358068487189346 28.032421792613338 22.570703247955294 42-43 22.771769456557035 26.423945790782494 27.80846844060454 22.995816312055936 44-45 22.731051468473215 26.60698473161095 28.060172815114992 22.60179098480084 46-47 23.065621202258985 26.737808663458534 27.42398874269611 22.772581391586368 48-49 22.822143319119988 26.82106198625872 27.376608971751775 22.98018572286951 50-51 22.927341881406306 26.92232877109192 27.426593338703626 22.723736008798152 52-53 23.20789019316879 26.68243390945474 27.353620301667036 22.756055595709434 54-55 22.830608868660196 26.8288314795034 27.482578839662867 22.857980812173537 56-57 22.849477637968704 26.942251031009874 27.3226740150145 22.885597316006926 58-59 22.771462895872613 26.64699147537974 27.408702810675322 23.172842818072333 60-61 22.564207430796014 26.824692766394854 27.603448074764724 23.007651728044408 62-63 22.6292040389325 26.889125004561993 27.570206683570177 22.911464272935337 64-65 22.424914765977274 26.844141055699772 27.454959018251547 23.275985160071404 66-67 22.415695428412434 26.88020269254654 27.355404441650172 23.348697437390854 68-69 22.835057471372487 26.581556222313818 27.48726720185838 23.09611910445532 70-71 22.629705931099043 26.60691741136894 27.205413289302417 23.557963368229604 72-73 22.607594227076575 26.70043868634519 27.172140792750753 23.51982629382748 74-75 22.6007597924774 26.942251031009874 27.016089435332795 23.440899741179933 76-77 22.568252082243 26.62695634146791 27.353338116682362 23.451453459606725 78-79 22.608736221377505 26.735954994881165 27.281117572938236 23.374191210803094 80-81 22.691256442531685 26.692572556023713 27.11938763474126 23.496783366703344 82-83 22.6702099500771 26.735849237630315 27.22333916585475 23.370601646437834 84-85 22.59319671067113 26.79634719690257 27.162944442249554 23.447511650176754 86-87 22.632995970420527 26.755117118178106 26.9815893049742 23.63029760642716 88-89 22.507431811879684 26.831653329350136 26.733377705354478 23.9275371534157 90-91 22.414802496054058 26.770932295380195 26.97431517479715 23.839950033768595 92-93 22.42811584540401 26.509659427394954 27.124637578645338 23.937587148555693 94-95 22.243281595000735 26.351633917826554 27.18221767047134 24.222866816701373 96-97 22.392337962797484 26.10410518953237 27.198117562779572 24.305439284890575 98-99 22.10725587894791 26.2260843522406 26.954855293658625 24.71180447515286 100-101 22.34372689610438 25.79163234983714 27.22594100226839 24.63869975179009 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 206.0 1 152.0 2 148.5 3 188.0 4 166.0 5 172.5 6 301.5 7 682.5 8 1107.0 9 1318.5 10 1228.0 11 1047.0 12 1071.5 13 1210.0 14 1364.0 15 1555.5 16 1764.0 17 1955.0 18 2189.5 19 2545.0 20 2969.0 21 3597.5 22 4502.5 23 5690.5 24 7185.0 25 9217.5 26 12127.5 27 15631.5 28 19141.5 29 22929.5 30 27248.0 31 33043.0 32 40960.0 33 45230.0 34 46710.5 35 50787.5 36 56442.5 37 62185.5 38 67232.0 39 71466.5 40 76019.0 41 81312.5 42 85396.0 43 89093.0 44 93002.5 45 97542.5 46 100678.0 47 102865.0 48 104193.5 49 103387.5 50 102491.0 51 100519.5 52 98534.0 53 98140.5 54 96631.5 55 90469.5 56 82462.5 57 74596.5 58 67737.0 59 60312.0 60 50299.5 61 41954.0 62 34814.5 63 26285.5 64 18756.0 65 13788.5 66 10321.5 67 7883.0 68 6097.5 69 4763.0 70 3637.0 71 2577.5 72 1840.5 73 1406.5 74 1087.0 75 792.0 76 589.0 77 396.0 78 228.0 79 151.0 80 97.0 81 52.0 82 30.0 83 15.0 84 8.0 85 6.0 86 2.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02554714727911594 2 0.012002268014783483 3 0.0018059839019109944 4 0.0 5 2.6337265236202003E-4 6 0.0 7 0.0 8 7.524932924629143E-5 9 3.009973169851657E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014297372556795372 26-27 0.007731868580056444 28-29 1.1287399386943715E-4 30-31 0.0 32-33 3.574343139198843E-4 34-35 0.009029919509554972 36-37 0.001072302941759653 38-39 0.011513147374682588 40-41 0.01119333772538585 42-43 0.004815957071762651 44-45 7.336809601513415E-4 46-47 3.7624664623145714E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.6436996934718575E-5 66-67 4.7030830778932144E-4 68-69 0.0011099276063827987 70-71 5.6436996934718575E-5 72-73 3.574343139198843E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.069356554273014E-4 82-83 0.0012228016002522359 84-85 0.005493201034979275 86-87 0.004119900776234455 88-89 0.0 90-91 0.0013544879264332458 92-93 4.891206401008943E-4 94-95 5.6436996934718575E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2657831.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.35527414649398 #Duplication Level Percentage of deduplicated Percentage of total 1 74.30206072673259 41.87313001904459 2 14.36933370290752 16.195754802596174 3 5.070039705647835 8.5717043263618 4 2.251883182847891 5.076219764610893 5 1.2047134111283224 3.3945977276047263 6 0.7124779391479729 2.409113375040784 7 0.44956985564479307 1.7734942724003426 8 0.29218594887854427 1.3172975400643068 9 0.2309304730819329 1.171273510738069 >10 1.0271929804009796 10.282234636385512 >50 0.05881327954984888 2.2785539905521897 >100 0.026524872165311176 2.7251579020920977 >500 0.0023373010207459605 0.9103304831969479 >1k 0.0018698408165514944 1.8174620705372797 >5k 6.678002916255336E-5 0.2036755787743101 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5412 0.20362468494046462 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4541 0.1708536020537047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3128 0.1176899509411998 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.524932924629143E-5 2 0.0 0.0 0.0 0.0 7.524932924629143E-5 3 0.0 0.0 0.0 0.0 1.1287399386943715E-4 4 0.0 0.0 0.0 3.7624664623145714E-5 1.1287399386943715E-4 5 0.0 0.0 0.0 3.7624664623145714E-5 1.1287399386943715E-4 6 0.0 0.0 0.0 3.7624664623145714E-5 1.1287399386943715E-4 7 0.0 0.0 0.0 3.7624664623145714E-5 1.1287399386943715E-4 8 0.0 0.0 0.0 3.7624664623145714E-5 1.1287399386943715E-4 9 0.0 0.0 0.0 3.7624664623145714E-5 1.1287399386943715E-4 10-11 0.0 0.0 0.0 5.6436996934718575E-5 1.1287399386943715E-4 12-13 0.0 0.0 0.0 1.6931099080415572E-4 2.257479877388743E-4 14-15 0.0 0.0 0.0 2.257479877388743E-4 2.6337265236202003E-4 16-17 0.0 0.0 0.0 2.6337265236202003E-4 3.3862198160831145E-4 18-19 0.0 0.0 0.0 4.891206401008943E-4 3.7624664623145713E-4 20-21 0.0 0.0 0.0 5.2674530472404E-4 4.514959754777486E-4 22-23 0.0 0.0 0.0 7.148686278397686E-4 4.891206401008943E-4 24-25 0.0 0.0 0.0 0.0010158659448249343 4.891206401008943E-4 26-27 0.0 0.0 0.0 0.0014109249233679642 5.2674530472404E-4 28-29 1.8812332311572857E-5 0.0 0.0 0.0034050321483946873 5.643699693471857E-4 30-31 3.7624664623145714E-5 0.0 0.0 0.010497281429857654 5.643699693471857E-4 32-33 3.7624664623145714E-5 0.0 0.0 0.025961018589970544 6.396192985934771E-4 34-35 3.7624664623145714E-5 0.0 0.0 0.05041705059501526 7.148686278397686E-4 36-37 3.7624664623145714E-5 0.0 0.0 0.09336560526233609 7.148686278397686E-4 38-39 7.524932924629143E-5 0.0 0.0 0.1556156128813307 7.148686278397686E-4 40-41 7.524932924629143E-5 0.0 0.0 0.22416775182470217 7.148686278397686E-4 42-43 7.524932924629143E-5 0.0 0.0 0.29633185857189565 7.148686278397686E-4 44-45 1.1287399386943715E-4 0.0 0.0 0.3861043083627213 7.336809601513415E-4 46-47 1.3168632618101E-4 0.0 0.0 0.4935039135294908 7.713056247744872E-4 48-49 1.5049865849258286E-4 0.0 0.0 0.6098017518796341 9.406166155786429E-4 50-51 1.5049865849258286E-4 0.0 0.0 0.735505756385564 9.406166155786429E-4 52-53 1.5049865849258286E-4 0.0 0.0 0.8557541845211377 9.782412802017886E-4 54-55 1.5049865849258286E-4 0.0 0.0 0.9903564222104415 9.782412802017886E-4 56-57 1.5049865849258286E-4 0.0 0.0 1.1477968313259947 9.970536125133616E-4 58-59 1.5049865849258286E-4 0.0 0.0 1.3191395540198003 0.0010158659448249343 60-61 1.5049865849258286E-4 0.0 0.0 1.5029548530361787 0.0010158659448249343 62-63 1.5049865849258286E-4 0.0 0.0 1.6879741413204978 0.0010911152740712257 64-65 1.8812332311572856E-4 0.0 1.8812332311572857E-5 1.887215552832366 0.0010911152740712257 66-67 2.257479877388743E-4 0.0 3.7624664623145714E-5 2.1125872939250088 0.0010911152740712257 68-69 2.257479877388743E-4 0.0 3.7624664623145714E-5 2.369432066974913 0.0010911152740712257 70-71 2.445603200504472E-4 0.0 3.7624664623145714E-5 2.638448419030405 0.0010911152740712257 72-73 2.821849846735929E-4 0.0 3.7624664623145714E-5 2.919899722743846 0.0011287399386943715 74-75 3.009973169851657E-4 0.0 3.7624664623145714E-5 3.211020565265436 0.0011663646033175173 76-77 3.009973169851657E-4 0.0 3.7624664623145714E-5 3.522232978695786 0.0011663646033175173 78-79 3.1980964929673855E-4 0.0 3.7624664623145714E-5 3.864974861080332 0.0012039892679406629 80-81 4.514959754777486E-4 0.0 3.7624664623145714E-5 4.229426174952433 0.0012039892679406629 82-83 4.514959754777486E-4 0.0 3.7624664623145714E-5 4.613893810404047 0.0012416139325638086 84-85 4.514959754777486E-4 0.0 3.7624664623145714E-5 5.003610086570591 0.0012416139325638086 86-87 4.514959754777486E-4 0.0 3.7624664623145714E-5 5.4089782232203625 0.0012604262648753814 88-89 4.891206401008943E-4 0.0 3.7624664623145714E-5 5.841041059420257 0.001298050929498527 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3495 0.0 51.92841 1 GTATCAA 5845 0.0 42.105038 1 ATCAACG 7640 0.0 31.768885 3 TCAACGC 7770 0.0 31.237358 4 TATCAAC 7875 0.0 31.06329 2 CAACGCA 7945 0.0 30.609095 5 AACGCAG 8290 0.0 29.391996 6 ACGCAGA 9370 0.0 25.700089 7 TATACCG 130 1.0030963E-5 25.575796 5 CGCAGAG 9550 0.0 25.016748 8 TAAGGTG 1495 0.0 22.557531 5 GTATAGC 1160 0.0 22.116917 1 TACATGG 6735 0.0 21.722202 2 GCAGAGT 10935 0.0 21.413832 9 GTACATG 7300 0.0 20.826485 1 GAGTACT 6805 0.0 20.659977 12-13 GTGGTAT 1935 0.0 20.624678 1 GTAAGGT 1535 0.0 20.42255 4 CAGAGTA 10135 0.0 20.034506 10-11 AGAGTAC 9755 0.0 19.98721 10-11 >>END_MODULE