##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938399_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 120490 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.048659639804132 31.0 31.0 33.0 26.0 34.0 2 29.936243671674 31.0 31.0 34.0 25.0 34.0 3 29.85876836251971 31.0 30.0 34.0 25.0 34.0 4 33.461299692920576 37.0 35.0 37.0 28.0 37.0 5 33.48395717486928 37.0 35.0 37.0 28.0 37.0 6 33.59688770852353 37.0 35.0 37.0 28.0 37.0 7 33.522400199186656 37.0 35.0 37.0 28.0 37.0 8 33.536559050543616 37.0 35.0 37.0 28.0 37.0 9 34.8007801477301 39.0 35.0 39.0 27.0 39.0 10-11 34.697609760146065 38.0 35.0 39.0 27.0 39.0 12-13 34.5753050045647 38.0 35.0 39.0 27.0 39.0 14-15 35.19515312474064 39.0 34.5 41.0 25.5 41.0 16-17 35.07944227736742 38.5 34.5 40.0 26.0 41.0 18-19 35.25082164494979 38.5 35.0 40.5 27.0 41.0 20-21 35.28962154535646 39.0 35.0 41.0 27.0 41.0 22-23 35.130629097850445 39.0 35.0 41.0 25.5 41.0 24-25 34.885853597808946 39.0 35.0 41.0 24.5 41.0 26-27 34.55409162586107 38.0 35.0 40.5 23.5 41.0 28-29 34.219802473234296 38.0 34.0 40.0 21.5 41.0 30-31 33.6320773508175 37.0 34.0 40.0 19.0 41.0 32-33 32.962386920076355 35.5 33.0 40.0 16.5 41.0 34-35 32.482882396879404 35.0 33.0 40.0 11.5 41.0 36-37 31.962362021744543 35.0 33.0 39.0 5.0 41.0 38-39 31.294717403933937 35.0 31.0 39.0 2.0 41.0 40-41 30.845493401942072 35.0 31.0 39.0 2.0 41.0 42-43 30.18013113121421 35.0 30.0 39.0 2.0 41.0 44-45 29.34603701551996 35.0 26.0 39.0 2.0 41.0 46-47 28.401767781558636 35.0 23.0 39.0 2.0 41.0 48-49 27.590181757822226 35.0 18.5 39.0 2.0 41.0 50-51 26.687463689932777 34.0 10.5 38.5 2.0 40.0 52-53 26.527595651091378 34.5 4.5 38.0 2.0 40.0 54-55 26.40626193045066 35.0 2.0 39.0 2.0 40.0 56-57 25.901132874097435 35.0 2.0 38.5 2.0 40.0 58-59 25.4344841895593 34.0 2.0 38.0 2.0 40.0 60-61 24.886015436965724 33.5 2.0 38.0 2.0 40.0 62-63 24.322931363598638 33.0 2.0 38.0 2.0 40.0 64-65 23.810785127396464 33.0 2.0 37.0 2.0 40.0 66-67 23.28384513237613 32.0 2.0 36.5 2.0 39.0 68-69 22.753821893933107 31.5 2.0 36.0 2.0 39.0 70-71 22.16479790854013 31.0 2.0 35.0 2.0 39.0 72-73 21.525645281766124 30.5 2.0 35.0 2.0 38.0 74-75 20.880541953689104 29.0 2.0 35.0 2.0 37.0 76-77 20.222781973607766 28.5 2.0 35.0 2.0 36.5 78-79 19.51956593908208 26.5 2.0 34.5 2.0 36.0 80-81 18.865752344592913 25.5 2.0 34.0 2.0 35.5 82-83 18.21551996016267 23.5 2.0 34.0 2.0 35.0 84-85 17.602742966221264 20.0 2.0 34.0 2.0 35.0 86-87 17.0110050626608 14.0 2.0 33.5 2.0 35.0 88-89 16.325636982322184 3.5 2.0 33.0 2.0 35.0 90-91 15.592812681550337 2.0 2.0 32.5 2.0 35.0 92-93 14.886173126400532 2.0 2.0 32.0 2.0 35.0 94-95 14.14930284670927 2.0 2.0 31.5 2.0 34.5 96-97 13.257452900655657 2.0 2.0 31.0 2.0 34.0 98-99 12.282853348825629 2.0 2.0 30.5 2.0 34.0 100-101 9.941874844385428 2.0 2.0 23.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7091423094571816 1101 2 0.865244077570182 1101 3 0.5433032100135442 1101 4 0.4445353980014488 1101 5 0.41431040244749084 1101 6 0.658052641445785 1101 7 0.8191071366634333 1101 8 0.8547209867851464 1101 9 0.7044534362767507 1101 10-11 0.7805327003424409 1101 12-13 1.270594206176476 1101 14-15 0.9473597769140056 1101 16-17 0.9504327866184852 1101 18-19 1.082091620756863 1101 20-21 1.1456493388659155 1101 22-23 0.7260325160682868 1101 24-25 0.9638943817062255 1101 26-27 0.7785372149350067 1101 28-29 0.7734920623823953 1101 30-31 0.9206131562700861 1101 32-33 0.9235842931088953 1101 34-35 0.39994850665625137 1101 36-37 1.070202277525361 1101 38-39 0.6089139060411703 1101 40-41 0.835982239809514 1101 42-43 1.1882742260460937 1101 44-45 1.755363885075223 1101 46-47 0.7238634656655734 1101 48-49 0.03069711805182962 1101 50-51 -0.19291952656869782 1101 52-53 -0.4147967466233702 1101 54-55 -0.341388407429168 1101 56-57 -0.6259653537788381 1101 58-59 -1.2270699208668994 1101 60-61 -1.0560261682998089 1101 62-63 -1.429026469872678 1101 64-65 -1.2841343486766803 1101 66-67 -1.2857329646066695 1101 68-69 -1.292647745049674 1101 70-71 -1.0434172194156943 1101 72-73 -0.7651740210930278 1101 74-75 -1.1256657804828656 1101 76-77 -0.6872438794246847 1101 78-79 -0.8875915056003016 1101 80-81 -1.2460462418487879 1101 82-83 -0.7655291098307657 1101 84-85 -0.5884146332040991 1101 86-87 0.10356831442022596 1101 88-89 0.6064459141556178 1101 90-91 1.2347591937373927 1101 92-93 1.0949564770248283 1101 94-95 0.0726607356395057 1101 96-97 0.6438853294146476 1101 98-99 0.23986610739601844 1101 100-101 0.5950060395880126 1102 1 0.7127137380286079 1102 2 0.8307202680463703 1102 3 0.3998508290611653 1102 4 0.12548777895383267 1102 5 0.40716754530463817 1102 6 0.5384097843029281 1102 7 0.4625595176158157 1102 8 0.45948289154704725 1102 9 1.0354058172291332 1102 10-11 1.2028541289138772 1102 12-13 0.9881537299860028 1102 14-15 0.9988478721520977 1102 16-17 0.6051946913803903 1102 18-19 0.7523297159949607 1102 20-21 0.5414826721992512 1102 22-23 0.9915087065444794 1102 24-25 0.9674658102776519 1102 26-27 0.886870548268341 1102 28-29 1.1993849195252508 1102 30-31 1.8247798229367547 1102 32-33 1.650965245489843 1102 34-35 1.6493532685610077 1102 36-37 2.2082975156206004 1102 38-39 2.5383781917554558 1102 40-41 2.3517560493333214 1102 42-43 2.2876789879508586 1102 44-45 2.3181615041228447 1102 46-47 2.0735658466179565 1102 48-49 2.24230426090897 1102 50-51 2.1895209496217767 1102 52-53 1.948298491471867 1102 54-55 2.155635402094642 1102 56-57 1.7594513128878297 1102 58-59 1.914299126752148 1102 60-61 1.711235736462097 1102 62-63 2.081092577746368 1102 64-65 2.414079937037606 1102 66-67 2.2130765592028503 1102 68-69 2.5505070168550894 1102 70-71 2.8833684948700196 1102 72-73 2.0532783598593554 1102 74-75 1.9832627909457052 1102 76-77 2.585672787241979 1102 78-79 2.85853944678065 1102 80-81 3.152763281960734 1102 82-83 3.1088756520739977 1102 84-85 3.3550377477482805 1102 86-87 3.529163552515463 1102 88-89 3.381743533203238 1102 90-91 3.336247288975489 1102 92-93 3.696742191310543 1102 94-95 4.201529783258556 1102 96-97 4.215611519890841 1102 98-99 4.579151821681732 1102 100-101 4.83369651577849 1103 1 1.0375826764296576 1103 2 0.7519194816767794 1103 3 0.8575015498999576 1103 4 1.4734563241700798 1103 5 1.406184583312502 1103 6 1.012443205010662 1103 7 0.9688668570653576 1103 8 1.0853020265404965 1103 9 1.3627321606105198 1103 10-11 1.7427869598444161 1103 12-13 1.6303146080987219 1103 14-15 1.2960710045243218 1103 16-17 1.3184974436739694 1103 18-19 1.309832992534929 1103 20-21 1.2207913222647804 1103 22-23 1.5096935033989993 1103 24-25 1.5118629269224755 1103 26-27 1.7818574421084534 1103 28-29 1.7738852471792512 1103 30-31 1.8158512515081853 1103 32-33 1.4535537120691373 1103 34-35 1.9572661125977007 1103 36-37 2.4343787738119502 1103 38-39 2.4331603018471952 1103 40-41 1.6597917636190367 1103 42-43 2.125408345749026 1103 44-45 2.6683826705710736 1103 46-47 2.0065196474043248 1103 48-49 2.7309592412497317 1103 50-51 2.694149062334752 1103 52-53 2.9779511782346475 1103 54-55 3.0906779971143017 1103 56-57 3.4037419419835047 1103 58-59 2.8520039105005104 1103 60-61 2.7201643078906663 1103 62-63 3.0493920534999717 1103 64-65 2.886638914757132 1103 66-67 3.151969088691711 1103 68-69 3.3151285764881173 1103 70-71 3.1042892026026543 1103 72-73 2.7435634188370734 1103 74-75 3.364897784392621 1103 76-77 2.707601031016555 1103 78-79 2.176322867488384 1103 80-81 2.075273111580657 1103 82-83 1.429403175009778 1103 84-85 1.0363123217980856 1103 86-87 1.3816373401956739 1103 88-89 1.1042615541730925 1103 90-91 1.4306525969702477 1103 92-93 1.851968272568735 1103 94-95 1.8586808317513448 1103 96-97 2.0162750192355308 1103 98-99 1.0223038531365152 1103 100-101 0.4979330819645966 1104 1 0.5787075268484863 1104 2 0.035844491648855836 1104 3 0.1399905806139614 1104 4 0.3956741143575542 1104 5 -0.017987734198470662 1104 6 0.40215202032777597 1104 7 0.42614647413755335 1104 8 1.1184891027271746 1104 9 1.0555921526328547 1104 10-11 0.3215264891623235 1104 12-13 0.6664534194269933 1104 14-15 0.27800159886017184 1104 16-17 0.43345556094561033 1104 18-19 0.7021744364918518 1104 20-21 0.47587708213713853 1104 22-23 0.6699248556128055 1104 24-25 0.8340807171099485 1104 26-27 1.0255351445416423 1104 28-29 0.33311939157494663 1104 30-31 0.38976429498644194 1104 32-33 0.34677269890599405 1104 34-35 0.5183750076914393 1104 36-37 0.8505335404653138 1104 38-39 0.9815847134945841 1104 40-41 1.164139589705993 1104 42-43 1.2284398575160758 1104 44-45 1.8601257898371308 1104 46-47 1.8545052876117438 1104 48-49 0.9269704099772937 1104 50-51 1.1981777819199166 1104 52-53 1.6158450753228024 1104 54-55 1.9998124207281833 1104 56-57 2.1183410644406244 1104 58-59 2.6037789404167455 1104 60-61 2.63755561223849 1104 62-63 1.9067913355103414 1104 64-65 1.461828384242576 1104 66-67 1.3753436399481949 1104 68-69 1.404039625550741 1104 70-71 1.5348436501495222 1104 72-73 1.6207473039587335 1104 74-75 0.873713101504709 1104 76-77 0.28314949531651834 1104 78-79 0.6529882045446236 1104 80-81 0.5522974434514154 1104 82-83 0.5876799999000859 1104 84-85 1.087568803648903 1104 86-87 1.1298623102794423 1104 88-89 1.357791669849199 1104 90-91 0.2119848666152393 1104 92-93 1.1452670360310417 1104 94-95 1.187360528600168 1104 96-97 0.2741130726858696 1104 98-99 1.7765120701289376 1104 100-101 1.8997679443499162 1105 1 0.16534065656462005 1105 2 0.19243809567328896 1105 3 -0.8204118627924366 1105 4 -0.2694354796057894 1105 5 0.32717935168007983 1105 6 0.687096325034922 1105 7 0.25129918703730425 1105 8 0.5285501878870704 1105 9 0.9111437156943012 1105 10-11 0.6321265610272135 1105 12-13 0.5998346626896662 1105 14-15 0.6418894896255409 1105 16-17 1.1568475839423726 1105 18-19 0.49775474551089616 1105 20-21 0.7304584691295943 1105 22-23 0.6820340902516833 1105 24-25 0.9177314537251178 1105 26-27 0.703724149212853 1105 28-29 0.9319336208239477 1105 30-31 1.2042810035743017 1105 32-33 0.9985154225854771 1105 34-35 1.3337540950072864 1105 36-37 1.175829983153065 1105 38-39 1.4219009190281824 1105 40-41 1.6845851520487933 1105 42-43 1.3419145251409397 1105 44-45 0.5907043022338208 1105 46-47 0.3208725172200779 1105 48-49 0.06635237188890031 1105 50-51 0.21007142187679761 1105 52-53 0.9053528007989051 1105 54-55 0.8559748353886221 1105 56-57 0.5182691995466229 1105 58-59 0.45455148802723855 1105 60-61 0.29454447317992205 1105 62-63 -0.25059278234808247 1105 64-65 -0.2514306887002924 1105 66-67 -0.006079284952992481 1105 68-69 -0.5241314128969741 1105 70-71 -0.424408954952888 1105 72-73 0.1754005558451901 1105 74-75 -0.06462681944390525 1105 76-77 0.9999658805123488 1105 78-79 0.7187445825539704 1105 80-81 0.9406912630705335 1105 82-83 1.2343921809996168 1105 84-85 1.4395271220103858 1105 86-87 0.8452054651482861 1105 88-89 0.30931879884668234 1105 90-91 0.002606497948335118 1105 92-93 0.8805526989846886 1105 94-95 0.8305513298937353 1105 96-97 0.9072068563725377 1105 98-99 0.5592285631221099 1105 100-101 0.3030737294738852 1106 1 -0.3821962732199857 1106 2 -0.39804801091875675 1106 3 -0.19400462650688866 1106 4 -0.9031961355568541 1106 5 -0.4075118697684772 1106 6 -0.6139383596790751 1106 7 -0.1511215847463916 1106 8 -0.5647015853933297 1106 9 -0.29839620751777574 1106 10-11 -0.27559778334709506 1106 12-13 -0.027418542229973752 1106 14-15 -0.14277895574441146 1106 16-17 0.020549022089056734 1106 18-19 -0.3566410376608289 1106 20-21 0.07909515813850732 1106 22-23 -0.11408746893358312 1106 24-25 0.16670653018766046 1106 26-27 -0.12704958671478295 1106 28-29 0.44660792289982965 1106 30-31 -0.2904479610902442 1106 32-33 -0.5097490402244418 1106 34-35 -0.6444459440374146 1106 36-37 -1.0256732380351963 1106 38-39 -0.4864741704492701 1106 40-41 0.12829570062691076 1106 42-43 -0.25218321677354893 1106 44-45 -0.2713702731549965 1106 46-47 -0.4969839284101667 1106 48-49 -0.44129388307302975 1106 50-51 0.11454204073538676 1106 52-53 0.3328597963087603 1106 54-55 -0.14041352928893147 1106 56-57 -1.2140793395306204 1106 58-59 -0.6451426617742833 1106 60-61 0.4563847803316108 1106 62-63 0.8391775046305057 1106 64-65 1.1420748751703371 1106 66-67 1.2390889326561698 1106 68-69 0.31650111134328185 1106 70-71 0.6715434105055635 1106 72-73 1.445362161884102 1106 74-75 1.7667976615868746 1106 76-77 1.3104689064770803 1106 78-79 0.6916735862632173 1106 80-81 1.4734888162689472 1106 82-83 1.0011000615228198 1106 84-85 0.9117728433613301 1106 86-87 0.4653980856988156 1106 88-89 0.8362921833719632 1106 90-91 0.6397835656908981 1106 92-93 0.35738617331279343 1106 94-95 0.7436768586241325 1106 96-97 0.4332366604982614 1106 98-99 1.094759245753723 1106 100-101 -0.5325155202170127 1107 1 -0.719645569330698 1107 2 -1.1167905544644512 1107 3 -1.190246573536239 1107 4 -0.7976723942063444 1107 5 -0.6783302901932018 1107 6 -0.5711681377749898 1107 7 -0.8120616945053953 1107 8 -0.7786123465481865 1107 9 -1.1453301135068017 1107 10-11 -0.8654629706532262 1107 12-13 -0.8171763565940822 1107 14-15 -0.9290471494929164 1107 16-17 -0.9667750055893052 1107 18-19 -1.1172590285937858 1107 20-21 -1.166580098363518 1107 22-23 -1.1313267912910163 1107 24-25 -0.9827506399387929 1107 26-27 -0.8279562915585004 1107 28-29 -0.15897547008513868 1107 30-31 -0.17657299091605694 1107 32-33 -0.6309611614365664 1107 34-35 -0.5873891556814179 1107 36-37 -0.7787154713923883 1107 38-39 -0.30915968703242314 1107 40-41 -0.15460217577490099 1107 42-43 0.23654262431449524 1107 44-45 0.9684591231704651 1107 46-47 0.7179110847131938 1107 48-49 -0.13716002480531486 1107 50-51 -0.6036338122829825 1107 52-53 -0.9548400366666598 1107 54-55 -0.7380334290741892 1107 56-57 -0.09674457455805907 1107 58-59 -0.04958074337772089 1107 60-61 -0.7381690254426694 1107 62-63 0.30106011021390344 1107 64-65 -0.5093507988931307 1107 66-67 -1.0136550425287503 1107 68-69 -1.1436217710236996 1107 70-71 -1.1509929769914535 1107 72-73 -1.0043515102705136 1107 74-75 -1.113869243686331 1107 76-77 -1.1928715850523908 1107 78-79 -0.8807733237821189 1107 80-81 -2.084790830593377 1107 82-83 -2.434684953986608 1107 84-85 -2.394150563940027 1107 86-87 -1.9440507331988215 1107 88-89 -2.3583809256712236 1107 90-91 -2.8589637499855503 1107 92-93 -2.988593306524953 1107 94-95 -2.424958311979543 1107 96-97 -2.056980471451153 1107 98-99 -2.2152204726905627 1107 100-101 -1.1941714495894775 1108 1 -0.874857690542818 1108 2 -0.9168511605250558 1108 3 -0.782792028081694 1108 4 -0.9413693639033127 1108 5 -1.1571181689810786 1108 6 -1.0928045014113579 1108 7 -1.255940482384183 1108 8 -0.5879456798815212 1108 9 -0.6814513256280108 1108 10-11 -0.8640387282289836 1108 12-13 -0.5490248414425665 1108 14-15 -0.36825927070503894 1108 16-17 -0.40480530861961483 1108 18-19 -0.5089559982907517 1108 20-21 -0.9187316135150283 1108 22-23 -1.3692055791698081 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-1.476291645312875 2316 96-97 -1.2404956229663053 2316 98-99 -1.4407053211754093 2316 100-101 -1.4322320556500827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4377.0 3 760.0 4 568.0 5 520.0 6 470.0 7 593.0 8 649.0 9 746.0 10 921.0 11 1248.0 12 1706.0 13 2232.0 14 2790.0 15 3267.0 16 3802.0 17 4173.0 18 3620.0 19 2635.0 20 2766.0 21 2601.0 22 2576.0 23 2498.0 24 2658.0 25 2670.0 26 2783.0 27 3127.0 28 3359.0 29 3671.0 30 4028.0 31 4360.0 32 5047.0 33 5638.0 34 7361.0 35 9185.0 36 7937.0 37 8588.0 38 4374.0 39 186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.52639442231076 23.095949535192563 16.420982735723772 13.956673306772908 2 15.473450702470068 27.221442161497496 37.75641611485448 19.548691021177955 3 20.20557051218134 33.63578954365673 31.580084421843317 14.57855552231861 4 11.734939759036145 22.805982550893226 36.92646447860407 28.532613211466558 5 9.015262352862937 43.68557558023542 32.14321340692957 15.155948659972069 6 22.591337287544235 41.31568891325658 17.384405788433103 18.708568010766086 7 19.618277516728376 42.964949275242006 22.02251481893503 15.394258389094592 8 25.20859104532133 33.14625864459407 19.839602826045446 21.805547484039153 9 26.081289744143188 20.700161051984924 22.862740540271297 30.35580866360059 10-11 22.094162584073736 32.293448476293285 24.682803288217222 20.92958565141576 12-13 26.39587796655207 32.593044691345725 26.434906083403913 14.576171258698286 14-15 22.92566902855435 31.88174787990486 30.608653059869077 14.58393003167171 16-17 15.084016869790457 35.517882642048285 30.93016305250224 18.467937435659017 18-19 14.21917614874654 32.20849090675113 38.785269808587216 14.787063135915115 20-21 16.89336754606678 30.248789683033973 38.73138852212617 14.126454248773074 22-23 15.960034536005447 30.981121423946075 38.989572782971095 14.06927125707739 24-25 15.500770160135183 32.20847051594502 38.881254177755444 13.409505146164353 26-27 14.651331769395254 31.72164670223588 40.27154393904794 13.355477589320932 28-29 13.8994648143458 33.35589223212692 39.52983379765914 13.214809155868151 30-31 14.48146994705699 32.42022215301568 39.10183743382124 13.996470466106093 32-33 13.81638219871377 33.42079806027593 39.4326141020265 13.330205638983802 34-35 14.415319650723921 32.95203059279774 38.855925693714944 13.776724062763398 36-37 14.212938743990236 32.47681206353951 39.23099917793887 14.079250014531382 38-39 13.860465502426308 33.335685613592304 38.89274017127368 13.911108712707708 40-41 13.538241838530771 33.13863704294111 38.92265285111753 14.400468267410583 42-43 13.466998754669987 33.044416770444165 38.03652968036529 15.452054794520548 44-45 14.405063291139243 32.09213529777962 37.87507781697448 15.62772359410666 46-47 15.100048561218262 32.940278002598234 37.08416862779279 14.875504808390714 48-49 14.835627752161265 32.36562397539708 36.921812680797025 15.876935591644637 50-51 13.859237001128893 32.50381831462912 36.94011886579454 16.69682581844744 52-53 14.464651830700657 33.0866667220041 35.7835365600591 16.665144887236146 54-55 14.517246960874239 31.742210748689516 35.80461606783403 17.935926222602212 56-57 15.186768490080519 31.633186685481864 35.369801610359424 17.810243214078195 58-59 15.980226539768976 30.62105433550963 35.449862822657295 17.948856302064094 60-61 15.481937462901547 30.980063342368407 35.85941813019638 17.67858106453367 62-63 14.768345777597006 30.786790127043552 35.90630071262259 18.538563382736854 64-65 14.939137002959107 31.37276873721213 35.303858460848886 18.384235798979876 66-67 15.419765588684509 30.673932532040638 34.48270137459327 19.423600504681584 68-69 15.250881925710727 30.239883793318118 35.000622535795806 19.508611745175347 70-71 16.225898175629396 30.188248479691165 34.688777733961516 18.897075610717916 72-73 16.058412339980407 28.86579109037475 35.69785976389327 19.377936805751574 74-75 15.599925281750068 30.377119611465098 34.44718872584629 19.575766380938546 76-77 16.13792301913107 29.032405351220547 34.54301688963419 20.286654740014196 78-79 16.38806118807115 29.244445182227903 34.30748416762809 20.06000946207286 80-81 17.199321057589753 28.45167100343206 33.74044977859671 20.608558160381467 82-83 17.191021028124652 28.50313118611571 33.686499587073534 20.619348198686104 84-85 17.355090657824295 28.669369732031324 32.90698494777163 21.068554662372748 86-87 17.8045804314803 28.681237496783407 32.55609326880774 20.958088802928554 88-89 18.649773966034715 28.524282565288324 31.540974623588063 21.284968845088898 90-91 19.36968912024641 28.01031146091483 31.281834144883497 21.33816527395526 92-93 20.461932495091347 28.188342929253096 30.918925201638846 20.43079937401671 94-95 20.42346493959397 27.521899779964297 31.069871715033003 20.984763565408727 96-97 20.513778530566835 27.358208583455802 30.72666284736925 21.401350038608115 98-99 21.245366507685475 27.54525567745497 29.376237500466985 21.833140314392573 100-101 22.219407565520363 26.19028832697267 30.240487037121632 21.349817070385335 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2984.0 1 1788.0 2 640.5 3 671.5 4 530.0 5 474.5 6 733.0 7 1265.0 8 1749.5 9 1826.5 10 1411.0 11 964.5 12 838.0 13 767.5 14 732.5 15 733.0 16 734.5 17 753.5 18 753.5 19 768.5 20 865.0 21 954.0 22 1020.5 23 1163.5 24 1321.5 25 1462.0 26 1707.0 27 2049.0 28 2291.0 29 2477.5 30 2672.5 31 2918.0 32 3238.5 33 3500.5 34 3863.5 35 4125.5 36 4084.5 37 4129.0 38 3857.0 39 3325.0 40 3031.5 41 2958.0 42 2900.5 43 2647.5 44 2509.0 45 2511.5 46 2646.5 47 3211.0 48 4274.0 49 4425.5 50 3425.0 51 2548.0 52 1912.0 53 1506.0 54 1258.5 55 1026.5 56 856.5 57 763.5 58 703.5 59 648.5 60 635.5 61 591.5 62 487.5 63 385.5 64 300.0 65 237.0 66 200.0 67 191.5 68 168.5 69 147.0 70 130.0 71 109.5 72 88.5 73 71.5 74 61.5 75 49.5 76 42.5 77 39.5 78 34.0 79 24.5 80 18.0 81 12.5 82 6.0 83 7.0 84 9.0 85 9.5 86 7.0 87 4.5 88 2.5 89 0.0 90 2.0 91 2.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008299443937256203 2 0.10706282679060503 3 0.1178521039090381 4 0.11619221512158685 5 0.16100921238277036 6 0.09295377209726949 7 0.029877998174122337 8 0.03236783135529919 9 0.02655822059921985 10-11 0.04979666362353722 12-13 0.0531164411984397 14-15 0.029463025977259524 16-17 0.03153788696157358 18-19 0.036102581127064486 20-21 0.05560627437961657 22-23 0.029877998174122337 24-25 0.05021163582040004 26-27 0.056021246576479375 28-29 0.05353141339530251 30-31 0.06432069051373558 32-33 0.05021163582040004 34-35 0.057681135363930616 36-37 0.05062660801726284 38-39 0.032782803552162 40-41 0.03817744211137854 42-43 0.03319777574902481 44-45 0.012449165905884305 46-47 0.01950369325255208 48-49 0.013694082496472736 50-51 0.014938999087061169 52-53 0.014109054693335548 54-55 0.016183915677649596 56-57 0.016598887874512407 58-59 0.02116358204000332 60-61 0.027803137189808286 62-63 0.016183915677649596 64-65 0.012034193709021496 66-67 0.014938999087061169 68-69 0.012449165905884305 70-71 0.031122914764710765 72-73 0.029877998174122337 74-75 0.031122914764710765 76-77 0.026143248402357044 78-79 0.007469499543530584 80-81 0.007054527346667774 82-83 0.007054527346667774 84-85 0.007884471740393395 86-87 0.017428832268238027 88-89 0.036102581127064486 90-91 0.034442692339613244 92-93 0.032782803552162 94-95 0.04564694165490912 96-97 0.0406672752925554 98-99 0.029463025977259524 100-101 0.0734500788447174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 120490.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.04382106398871 #Duplication Level Percentage of deduplicated Percentage of total 1 83.43179933665009 40.083824383766284 2 7.621614151464898 7.323429330234875 3 2.91079325594251 4.195368910283011 4 1.6013681592039801 3.0774338119346005 5 1.0122996130458817 2.431737073616068 6 0.6374378109452736 1.8374968877085236 7 0.4819651741293532 1.6208814009461368 8 0.36276948590381425 1.3943065814590423 9 0.24875621890547264 1.0756079342684042 >10 1.5322692095080155 13.286579799153456 >50 0.09155610834715312 2.9919495393808617 >100 0.05009673852957435 5.093368744294132 >500 0.008637368711995578 3.145489252220101 >1k 0.008637368711995578 12.4425263507345 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4165 3.456718399867209 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3782 3.1388496970702966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2939 2.4392065731595984 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2374 1.970287990704623 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1732 1.4374636899327746 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 985 0.817495227819736 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 847 0.7029629014856005 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 677 0.561872354552245 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 677 0.561872354552245 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 604 0.5012864138102747 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 481 0.3992032533820234 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 444 0.36849531081417547 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 405 0.3361274794588762 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 306 0.25396298448003984 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 286 0.23736409660552743 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 275 0.2282347082745456 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 273 0.22657481948709438 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 273 0.22657481948709438 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 254 0.21080587600630757 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 229 0.19005726616316707 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 227 0.18839737737571582 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 221 0.1834177110133621 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 200 0.16598887874512408 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 179 0.14856004647688603 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 175 0.14524026890198358 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 175 0.14524026890198358 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 159 0.13196115860237362 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 155 0.12864138102747116 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.12781143663374556 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.12781143663374556 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 153 0.12698149224001992 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.11121254875923314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 131 0.10872271557805628 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 126 0.10457299360942818 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.299443937256204E-4 0.0 18-19 0.0 0.0 0.0 0.0012449165905884306 0.0 20-21 0.0 0.0 0.0 0.004564694165490912 0.0 22-23 0.0 0.0 0.0 0.02240849863059175 0.0 24-25 0.0 0.0 0.0 0.05270146900157689 0.0 26-27 0.0 0.0 0.0 0.10664785459374222 0.0 28-29 0.0 0.0 0.0 0.22615984729023159 0.0 30-31 0.0 0.0 0.0 0.46933355465183835 0.0 32-33 0.0 0.0 0.0 0.7747530915428666 0.0 34-35 0.0 0.0 0.0 1.2079840650676403 0.0 36-37 0.0 0.0 0.0 1.7237945057681134 0.0 38-39 0.0 0.0 0.0 2.273217694414474 0.0 40-41 0.0 0.0 0.0 2.8147564113204417 0.0 42-43 0.0 0.0 0.0 3.2546269399950205 0.0 44-45 0.0 0.0 0.0 3.6891028301103823 0.0 46-47 0.0 0.0 0.0 4.211967798157524 0.0 48-49 0.0 0.0 0.0 4.674246825462694 0.0 50-51 0.0 0.0 0.0 5.114532326334135 0.0 52-53 0.0 0.0 0.0 5.517885301684787 0.0 54-55 0.0 0.0 0.0 5.949871358618973 0.0 56-57 0.0 0.0 0.0 6.484770520375134 0.0 58-59 0.0 0.0 0.0 6.994771350319528 0.0 60-61 0.0 0.0 0.0 7.500207486098431 0.0 62-63 0.0 0.0 0.0 7.9894597061996855 0.0 64-65 0.0 0.0 0.0 8.50858992447506 0.0 66-67 0.0 0.0 0.0 9.083326417130053 0.0 68-69 0.0 0.0 0.0 9.702879907046228 0.0 70-71 0.0 4.149721968628102E-4 0.0 10.3514814507428 0.0 72-73 0.0 8.299443937256204E-4 0.0 10.928292804382107 0.0 74-75 0.0 8.299443937256204E-4 0.0 11.570669765125736 0.0 76-77 0.0 8.299443937256204E-4 0.0 12.173209394970538 0.0 78-79 0.0 8.299443937256204E-4 0.0 12.891526267740062 0.0 80-81 0.0 8.299443937256204E-4 0.0 13.628101917171549 0.0 82-83 0.0 8.299443937256204E-4 0.0 14.273798655490083 0.0 84-85 0.0 8.299443937256204E-4 0.0 14.88629761805959 0.0 86-87 0.0 8.299443937256204E-4 0.0 15.51830027388165 0.0 88-89 0.0 8.299443937256204E-4 0.0 16.192630093783716 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACA 40 5.3492055E-4 47.535324 4 TTCTAGG 45 9.5361186E-4 42.2712 1 CTAGGAC 75 6.8358186E-6 38.028263 3 GACAATG 55 0.0025559172 34.585526 7 TTTGTAC 55 0.0025611375 34.57115 3 ATGGGAT 155 3.6379788E-11 33.73475 5 CTTGCTA 105 1.8660558E-6 31.703396 9 AACCTTG 60 0.0039307186 31.677052 6 GGACAAT 60 0.0039307186 31.677052 6 ACTTGCT 120 5.2814794E-6 27.740473 8 CAATGGG 70 0.008315095 27.17434 9 GGCCATA 35 0.008399207 27.12922 54-55 AACTTGC 145 7.71106E-7 26.237293 7 GTACAAG 200 6.9303496E-10 26.155302 1 GGTATCA 2940 0.0 25.55682 1 GAGTACT 2385 0.0 24.126484 12-13 CATGGGA 685 0.0 23.594175 4 GTACATG 1355 0.0 23.163366 1 GTACTTT 2435 0.0 23.123596 14-15 TACATGG 1340 0.0 22.70344 2 >>END_MODULE