##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938399_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 120490 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.612905635322434 31.0 31.0 34.0 30.0 34.0 2 31.7830027388165 31.0 31.0 34.0 30.0 34.0 3 31.755938252137106 31.0 31.0 34.0 30.0 34.0 4 35.50907129222342 37.0 35.0 37.0 33.0 37.0 5 35.45102498132625 37.0 35.0 37.0 33.0 37.0 6 35.519005726616314 37.0 35.0 37.0 33.0 37.0 7 35.44190389243921 37.0 35.0 37.0 33.0 37.0 8 35.4866129969292 37.0 35.0 37.0 33.0 37.0 9 36.89804963067475 39.0 37.0 39.0 32.0 39.0 10-11 36.871109635654406 39.0 37.0 39.0 32.0 39.0 12-13 36.7731346999751 39.0 37.0 39.0 32.0 39.0 14-15 37.49575068470413 39.0 36.0 41.0 32.0 41.0 16-17 37.45297120092954 39.0 36.5 41.0 32.0 41.0 18-19 37.72908955100009 39.0 36.5 41.0 33.0 41.0 20-21 37.768428915262675 40.0 36.0 41.0 33.0 41.0 22-23 37.58669184164661 39.5 36.0 41.0 32.5 41.0 24-25 37.3396962403519 39.0 35.0 41.0 32.0 41.0 26-27 36.994958087808115 39.0 35.0 41.0 31.5 41.0 28-29 36.72615984729023 39.0 35.0 40.5 31.0 41.0 30-31 36.05756909287078 38.0 35.0 40.0 30.5 41.0 32-33 35.36370238194041 36.5 34.5 40.0 30.0 41.0 34-35 34.70170968545108 36.0 34.0 40.0 27.5 41.0 36-37 34.30448169972612 36.0 33.0 40.0 24.5 41.0 38-39 33.77668686198025 35.0 33.0 40.0 22.5 41.0 40-41 33.396526682712256 35.0 33.0 40.0 21.0 41.0 42-43 32.83068304423604 35.0 33.0 40.0 15.5 41.0 44-45 31.98977093534733 35.0 32.5 40.0 2.0 41.0 46-47 30.987198107726783 35.0 31.0 40.0 2.0 41.0 48-49 30.150381774421113 35.0 29.5 40.0 2.0 41.0 50-51 29.4491534567184 35.0 27.0 39.0 2.0 41.0 52-53 28.828446344094946 35.0 25.0 39.0 2.0 41.0 54-55 28.33036351564445 35.0 23.0 39.0 2.0 40.5 56-57 27.695750684704123 35.0 20.5 39.0 2.0 40.0 58-59 26.944758901153623 34.5 12.0 38.0 2.0 40.0 60-61 26.170785957340858 34.0 2.0 38.0 2.0 40.0 62-63 25.419520292140426 33.0 2.0 38.0 2.0 40.0 64-65 24.725151464851855 33.0 2.0 37.0 2.0 40.0 66-67 23.978060419951863 33.0 2.0 36.5 2.0 39.0 68-69 23.381417545024483 32.0 2.0 36.0 2.0 39.0 70-71 22.885529919495397 31.5 2.0 35.5 2.0 38.5 72-73 22.46128724375467 31.0 2.0 35.0 2.0 38.0 74-75 21.93421445763134 30.5 2.0 35.0 2.0 37.0 76-77 20.852274047638808 28.5 2.0 34.0 2.0 36.0 78-79 21.287252054112376 30.0 2.0 35.0 2.0 36.0 80-81 20.890127811436635 29.0 2.0 35.0 2.0 36.0 82-83 20.539310316208812 29.0 2.0 34.5 2.0 35.5 84-85 20.080425761473983 29.0 2.0 34.0 2.0 35.0 86-87 19.626259440617478 27.5 2.0 34.0 2.0 35.0 88-89 19.047423022657483 26.0 2.0 34.0 2.0 35.0 90-91 18.59779649763466 25.5 2.0 34.0 2.0 35.0 92-93 18.015656900987636 24.0 2.0 33.5 2.0 35.0 94-95 17.451269814922398 21.0 2.0 33.0 2.0 35.0 96-97 16.71935845298365 9.5 2.0 33.0 2.0 35.0 98-99 15.879164245995518 2.0 2.0 33.0 2.0 35.0 100-101 13.282135446925057 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3158370084401696 1101 2 0.2914427122186254 1101 3 0.8800580530004645 1101 4 0.4497191191651808 1101 5 0.4862354419840784 1101 6 0.3102843334004888 1101 7 0.2217923088916507 1101 8 0.45585248446175086 1101 9 0.44079403087237523 1101 10-11 0.544153650374831 1101 12-13 0.8520169255953931 1101 14-15 0.8782358386651481 1101 16-17 1.0967566297011189 1101 18-19 0.9766588484072898 1101 20-21 0.963050676236513 1101 22-23 0.7040583655199057 1101 24-25 0.9610881372214592 1101 26-27 0.9119887527599317 1101 28-29 0.8337771296038525 1101 30-31 0.8234754630188377 1101 32-33 1.1466590872567508 1101 34-35 1.097092131631257 1101 36-37 0.9293584191681532 1101 38-39 1.5304373541469758 1101 40-41 1.5305297994837161 1101 42-43 1.195060683857001 1101 44-45 1.2852492052557594 1101 46-47 1.6595492992593037 1101 48-49 2.3905624538073056 1101 50-51 1.2761807805190948 1101 52-53 1.2153848591161172 1101 54-55 1.721095584151346 1101 56-57 1.0369765599788785 1101 58-59 1.5636596310301236 1101 60-61 1.250457222363 1101 62-63 1.5947945762676987 1101 64-65 1.2222119168649357 1101 66-67 1.1804072703325694 1101 68-69 1.5005511360369574 1101 70-71 1.0327766478693476 1101 72-73 1.404733811321929 1101 74-75 0.5231046435291198 1101 76-77 0.5662133724506191 1101 78-79 0.3085019877839059 1101 80-81 0.5310540053189818 1101 82-83 -0.7659117523441061 1101 84-85 -0.7404705910358622 1101 86-87 -0.34186728864215965 1101 88-89 -0.1492942393469825 1101 90-91 0.15848796997061854 1101 92-93 0.3325790015092771 1101 94-95 0.07448565880148195 1101 96-97 -0.5526951898087056 1101 98-99 0.06892158600240705 1101 100-101 1.0683425572806033 1102 1 0.6039322465354076 1102 2 0.5515617598376679 1102 3 0.7508913863337945 1102 4 0.4437667382128012 1102 5 0.6034973467459821 1102 6 0.4977843334004888 1102 7 0.5878637374630813 1102 8 0.4147810558903231 1102 9 0.8181749832533285 1102 10-11 0.6911774598986327 1102 12-13 0.7073740684525376 1102 14-15 1.1020453624746693 1102 16-17 0.6732447249392166 1102 18-19 1.1593969436453904 1102 20-21 1.11811020004604 1102 22-23 0.7683440798056154 1102 24-25 0.6292428991262184 1102 26-27 0.8140720860932689 1102 28-29 1.1819914153181443 1102 30-31 0.9514516534950275 1102 32-33 1.22523051582818 1102 34-35 0.7836992744884057 1102 36-37 0.9966203239300597 1102 38-39 1.66883021128983 1102 40-41 1.0814226566265717 1102 42-43 1.7608344933808056 1102 44-45 2.0010230147795696 1102 46-47 1.9631207278307272 1102 48-49 2.34800292999779 1102 50-51 2.879156970995286 1102 52-53 2.885325335306593 1102 54-55 2.2008574889132504 1102 56-57 2.9161432266455485 1102 58-59 2.364552488172979 1102 60-61 2.531409603315378 1102 62-63 2.8507469572200783 1102 64-65 2.755247631150649 1102 66-67 3.0955858417611424 1102 68-69 2.9002535169893413 1102 70-71 3.578014743107442 1102 72-73 3.0999719065600218 1102 74-75 3.2894736911481637 1102 76-77 3.008772896260144 1102 78-79 3.5076091306410504 1102 80-81 3.8248040053189847 1102 82-83 3.77605253337018 1102 84-85 3.2208389327736597 1102 86-87 3.6843231875483156 1102 88-89 3.7459438558911096 1102 90-91 2.5016427318753784 1102 92-93 2.374840906271185 1102 94-95 3.0396642302300556 1102 96-97 2.363078619715104 1102 98-99 2.5317192050500275 1102 100-101 2.073104462042508 1103 1 0.11540013644366454 1103 2 -0.08924754553585501 1103 3 0.38875180573091583 1103 4 0.21293449705946443 1103 5 -0.11857014997747939 1103 6 -0.1615374228249351 1103 7 0.223102924881168 1103 8 0.06345405720093567 1103 9 0.23380407892829425 1103 10-11 0.58329738126168 1103 12-13 0.39159752847874785 1103 14-15 0.24817904530560497 1103 16-17 0.08989773935598322 1103 18-19 0.06953783486426346 1103 20-21 -0.1352892101767722 1103 22-23 0.02882900772173258 1103 24-25 -0.44738717951073426 1103 26-27 -0.06490071862494773 1103 28-29 0.3147731977558905 1103 30-31 0.47659909779385146 1103 32-33 0.17051229826592618 1103 34-35 0.5533175575814582 1103 36-37 0.5376589871017501 1103 38-39 0.2899393200709639 1103 40-41 1.0767945439135929 1103 42-43 1.310351203734676 1103 44-45 0.7566340894846775 1103 46-47 0.7755388143313873 1103 48-49 0.7875917242310777 1103 50-51 0.022096027351771852 1103 52-53 0.2876434873380518 1103 54-55 -0.18266151501859795 1103 56-57 -0.5634603120176251 1103 58-59 -0.09191048692531467 1103 60-61 -0.21302027872918217 1103 62-63 0.0032698929998957738 1103 64-65 -0.39862687736835767 1103 66-67 -0.5731969236385943 1103 68-69 0.09565816967610274 1103 70-71 0.47349311794361526 1103 72-73 0.13142669030838405 1103 74-75 0.3754419087104175 1103 76-77 0.06197571408452163 1103 78-79 0.7884495631443258 1103 80-81 1.2965848047947368 1103 82-83 1.281663608075288 1103 84-85 1.0500492866399753 1103 86-87 0.9821606711656159 1103 88-89 0.29967911146122717 1103 90-91 -0.024037150169181842 1103 92-93 0.8822950347115501 1103 94-95 1.2833978475301855 1103 96-97 1.4306162999248002 1103 98-99 1.2744698603580247 1103 100-101 0.5042971225929662 1104 1 0.07567137697019177 1104 2 0.15893753623518592 1104 3 0.30283238012261293 1104 4 -0.49396618104185563 1104 5 -0.24254302592482446 1104 6 0.06183712843154865 1104 7 0.39446311634506515 1104 8 0.24343012421330457 1104 9 0.22423088387444068 1104 10-11 0.14995075182410034 1104 12-13 0.04145791938421439 1104 14-15 0.07223169787840078 1104 16-17 0.7087649112746206 1104 18-19 0.3750025337074945 1104 20-21 0.3669844236485247 1104 22-23 0.16492793073729217 1104 24-25 0.1757879301821248 1104 26-27 0.4819680488261824 1104 28-29 0.07787650848584349 1104 30-31 0.4363119019422328 1104 32-33 0.13796343508283115 1104 34-35 0.31510455399150317 1104 36-37 0.5838097649238421 1104 38-39 0.3627354907929359 1104 40-41 0.15972234606756786 1104 42-43 0.5965099592193184 1104 44-45 1.0196177352764622 1104 46-47 -0.31995380633075854 1104 48-49 0.40091442068101557 1104 50-51 0.7132408219269628 1104 52-53 0.4779107390333017 1104 54-55 0.39045376220517625 1104 56-57 0.3272457111175946 1104 58-59 0.15806956891832513 1104 60-61 0.15873879586196082 1104 62-63 0.14096434852442385 1104 64-65 -0.9293408781661228 1104 66-67 -0.5444374501643239 1104 68-69 -0.2110430668615919 1104 70-71 -0.39682997738945147 1104 72-73 -0.847233062384074 1104 74-75 -0.49200922810649317 1104 76-77 -0.5720889049820919 1104 78-79 -0.25423093147075093 1104 80-81 -0.30497083940151626 1104 82-83 -0.3510049200459697 1104 84-85 -0.6433691417605054 1104 86-87 -0.8458010360541657 1104 88-89 -0.6962921689536081 1104 90-91 -1.239234597317168 1104 92-93 -1.2626590937288142 1104 94-95 -1.108744154863114 1104 96-97 -0.8878918771793067 1104 98-99 -0.8809748943288547 1104 100-101 -0.6095042336096661 1105 1 0.4117834862048255 1105 2 0.3163935189876099 1105 3 0.5146900876324985 1105 4 0.35684229901563924 1105 5 0.20776239987467449 1105 6 0.4448770134477158 1105 7 0.23477932187866202 1105 8 0.24026806887733443 1105 9 0.4056897412226306 1105 10-11 0.2265227963803369 1105 12-13 0.023563560778391945 1105 14-15 0.2319154923448039 1105 16-17 0.4315460826723907 1105 18-19 0.5881897417563735 1105 20-21 0.2822557136233712 1105 22-23 0.4486864646934592 1105 24-25 0.3611274918062719 1105 26-27 0.01592421124085064 1105 28-29 0.17439106112687597 1105 30-31 -0.32312036539516953 1105 32-33 -0.33350620199944814 1105 34-35 0.10464821191461482 1105 36-37 -0.0027155674512897576 1105 38-39 -0.013836553763297843 1105 40-41 -0.3234253362896027 1105 42-43 -0.6209172775755079 1105 44-45 -0.8839755094235002 1105 46-47 -0.6911000513900483 1105 48-49 0.12283321335079478 1105 50-51 0.15402414927155306 1105 52-53 -0.27217909208419755 1105 54-55 0.055491491274526794 1105 56-57 0.5800304757600685 1105 58-59 0.27995242914110463 1105 60-61 -0.4113294757873369 1105 62-63 -0.7308646130278582 1105 64-65 -0.19667828384344688 1105 66-67 0.08064339784142405 1105 68-69 -0.36336070969306533 1105 70-71 0.02990376317828236 1105 72-73 -0.022224079272326946 1105 74-75 -0.0089693430903246 1105 76-77 -0.21765124777763845 1105 78-79 0.44699077172723634 1105 80-81 0.4961071340084757 1105 82-83 0.5873350009026446 1105 84-85 0.9551216954655111 1105 86-87 1.2182665169461941 1105 88-89 0.8279981652181441 1105 90-91 0.8189759668222507 1105 92-93 0.734861567428208 1105 94-95 1.5132105702536656 1105 96-97 0.9160966244376532 1105 98-99 0.698004624176356 1105 100-101 0.5317821479929208 1106 1 -0.039768540866166546 1106 2 0.3014914561256319 1106 3 0.18684470466900294 1106 4 0.11938541087872068 1106 5 0.45803896654350496 1106 6 -0.019299468624254246 1106 7 0.2712861221649874 1106 8 0.19803471168334852 1106 9 0.3889005175615381 1106 10-11 0.29192267924621973 1106 12-13 0.21995843290697792 1106 14-15 0.45918821961753054 1106 16-17 0.38524556858375547 1106 18-19 0.4426505994384158 1106 20-21 0.5913596376163426 1106 22-23 0.5363418300868332 1106 24-25 0.21005701611159822 1106 26-27 0.39312861027774204 1106 28-29 0.1594239237545878 1106 30-31 -0.030480292511711582 1106 32-33 0.8576827093039938 1106 34-35 0.8707704218449734 1106 36-37 1.450430785122407 1106 38-39 1.6294249806936492 1106 40-41 2.0855354237806765 1106 42-43 2.008971445011859 1106 44-45 2.152140843800943 1106 46-47 1.0082557109578403 1106 48-49 1.7726021988391807 1106 50-51 1.6870169260009078 1106 52-53 2.1982120057227945 1106 54-55 1.1143839231089778 1106 56-57 1.0648355776016736 1106 58-59 1.3863817907601579 1106 60-61 1.2299472861050305 1106 62-63 0.026928059582285613 1106 64-65 -0.47025152520480873 1106 66-67 -0.17223614923998198 1106 68-69 -0.0406562130444037 1106 70-71 0.27064691408606834 1106 72-73 0.7838864172461868 1106 74-75 1.652838426806209 1106 76-77 1.6859734024468693 1106 78-79 1.8693568809222647 1106 80-81 0.7687367949702768 1106 82-83 1.4448868978246239 1106 84-85 1.123046469331591 1106 86-87 1.425886742103998 1106 88-89 1.928733507184699 1106 90-91 1.8953083674209381 1106 92-93 1.7562385440664592 1106 94-95 1.7023821717373657 1106 96-97 1.9157712518860812 1106 98-99 2.080545133059026 1106 100-101 1.8762540683417193 1107 1 -0.214249571646409 1107 2 0.04642106719697736 1107 3 -0.15955233660992718 1107 4 -0.29054062109455714 1107 5 -0.2447169389683026 1107 6 -0.27413125101509195 1107 7 0.030233867333208764 1107 8 -0.10064102203175906 1107 9 0.012439052517393634 1107 10-11 0.20930516552633804 1107 12-13 0.06695199053046252 1107 14-15 -0.3627814773521649 1107 16-17 -0.12802692008243355 1107 18-19 0.034126380874830886 1107 20-21 0.04465240783824953 1107 22-23 -0.14669921023767785 1107 24-25 0.09528727142059523 1107 26-27 -0.04915843338725523 1107 28-29 -0.12726183143509928 1107 30-31 0.19306420760758414 1107 32-33 0.1981742387719052 1107 34-35 0.24893195847108274 1107 36-37 -0.08536019555046437 1107 38-39 -0.3072249835153613 1107 40-41 0.3026077215616425 1107 42-43 0.3064243202206356 1107 44-45 1.0774569974635497 1107 46-47 1.0172332819432839 1107 48-49 0.039155527400382084 1107 50-51 -0.6324772281389137 1107 52-53 -0.30420388547262434 1107 54-55 -0.27370961065384947 1107 56-57 -0.544625171622851 1107 58-59 -0.5278988105283169 1107 60-61 0.09039228729806226 1107 62-63 -0.209859103385984 1107 64-65 -0.20322098356796658 1107 66-67 -0.5295494396241409 1107 68-69 -0.38884280335698307 1107 70-71 -0.27966923957653833 1107 72-73 -1.1981882666001518 1107 74-75 -2.2885836681591947 1107 76-77 -1.3977476665104192 1107 78-79 -1.109138704856786 1107 80-81 -2.0173226180576407 1107 82-83 -1.5641801506125041 1107 84-85 -0.9374402880055612 1107 86-87 -0.917408414183285 1107 88-89 -0.4412855813383274 1107 90-91 -0.8342609477783007 1107 92-93 -0.611901517971237 1107 94-95 -0.27811174379592174 1107 96-97 -0.29154800366152145 1107 98-99 -0.8109485438677204 1107 100-101 -0.423107659169613 1108 1 0.34593224653540844 1108 2 0.2956331884090986 1108 3 0.3699628149052252 1108 4 0.6333381667842346 1108 5 0.4732830610317009 1108 6 0.22114147625763536 1108 7 0.46750659460593624 1108 8 0.42499534160460684 1108 9 0.5383178403961821 1108 10-11 0.3948203170414999 1108 12-13 0.7765883541668259 1108 14-15 0.8351882196175353 1108 16-17 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-1.4863899271450691 2316 50-51 -1.151628743290427 2316 52-53 -1.153567521836262 2316 54-55 -0.6592853682296074 2316 56-57 -0.9609282019258814 2316 58-59 -0.9266260832555915 2316 60-61 -0.3303046823989071 2316 62-63 -0.4282530427799216 2316 64-65 -0.10464522599220771 2316 66-67 0.09069298461828268 2316 68-69 -0.28478219729637644 2316 70-71 -0.47769954260684244 2316 72-73 -0.9988852362971237 2316 74-75 -0.7447048802804055 2316 76-77 -0.4202628180255701 2316 78-79 -0.7629265836446635 2316 80-81 -1.027231708966731 2316 82-83 -0.9944831809155339 2316 84-85 -0.7778039243691985 2316 86-87 -0.7171053838802521 2316 88-89 -0.47977042982317286 2316 90-91 -0.6773215538389046 2316 92-93 -0.8626590937288157 2316 94-95 -1.530657198341375 2316 96-97 -2.1374570945706104 2316 98-99 -2.002887937807115 2316 100-101 -2.698895537957492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 9.0 5 25.0 6 75.0 7 173.0 8 242.0 9 451.0 10 788.0 11 1214.0 12 1494.0 13 1976.0 14 2437.0 15 3236.0 16 3876.0 17 4699.0 18 4109.0 19 3128.0 20 2988.0 21 2829.0 22 2719.0 23 2930.0 24 2822.0 25 2813.0 26 2495.0 27 2436.0 28 2741.0 29 3099.0 30 3449.0 31 4089.0 32 4649.0 33 5720.0 34 7329.0 35 10138.0 36 10124.0 37 12319.0 38 6620.0 39 244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00522864968047 25.013694082496475 16.610507096024566 15.370570171798489 2 14.608607561301678 27.909390595288507 38.27151267551835 19.210489167891453 3 18.788281185160592 34.53149638974189 33.47082745456054 13.209394970536975 4 10.54195368910283 25.439455556477714 34.74147232135447 29.277118433064985 5 7.917092249819387 44.88594371507104 32.81931192546275 14.37765210964683 6 20.57764129803303 42.615984729023154 16.660303759648105 20.146070213295708 7 17.623039256369825 43.41190140260602 20.56851190970205 18.3965474313221 8 24.39372562038343 34.5480952776164 20.297950037347498 20.76022906465267 9 23.927296871109636 20.61581874014441 21.517138351730434 33.939746037015524 10-11 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34.890187420109896 38.38003618917977 13.144723517986687 36-37 12.948681763846459 34.48914198432727 38.68956700757072 13.872609244255546 38-39 13.062261120725072 33.264444804103434 38.768057301976604 14.905236773194892 40-41 12.666663900173875 33.315766104100355 39.33081305839928 14.68675693732649 42-43 13.619029044024582 32.82153216670332 38.41911535859971 15.140323430672382 44-45 13.300132802124834 32.6062416998672 38.27896746347941 15.814658034528554 46-47 14.846875259357622 32.33048385758154 36.847041248236366 15.975599634824466 48-49 14.452651672337954 31.63250062245829 36.533737239604946 17.381110465598802 50-51 12.5958601686475 31.93969523935994 37.764756656264524 17.69968793572804 52-53 13.643455888455474 32.76786455307494 36.8901983567101 16.69848120175948 54-55 13.535070173545694 31.609219251869497 35.79846788450206 19.05724269008275 56-57 15.083824383766286 32.441696406340775 33.998257116773175 18.47622209311976 58-59 14.296565005893779 33.13258512775389 34.96131688609234 17.60953298025999 60-61 14.382106398871276 34.95518300273881 33.27620549423189 17.38650510415802 62-63 13.149376250197125 35.25701147898839 33.313966517542184 18.2796457532723 64-65 13.289949325375911 35.442355021187055 32.85550054160839 18.412195111828645 66-67 13.825487201620131 34.204126689020946 32.02458417715216 19.945801932206766 68-69 13.807843951027184 33.2737082382237 32.57356297987134 20.344884830877774 70-71 14.97053697402274 32.68445514150552 32.16864470080505 20.176363183666695 72-73 14.510807995717471 31.267039866544387 33.23443121599807 20.98772092174007 74-75 13.947204582243804 32.34964512514008 31.783505582534342 21.91964471008177 76-77 14.0541640199119 30.915756390615336 33.245730940251846 21.78434864922092 78-79 14.256598855188388 31.0248928036262 32.90025859957744 21.818249741607968 80-81 14.629337539432177 30.505977087829983 32.29661298356301 22.56807238917483 82-83 14.346737736154527 30.511725220120304 32.40054630517537 22.7409907385498 84-85 14.414018078739636 30.486499049579574 31.576784839756627 23.522698031924165 86-87 14.357154714475431 30.850763612217797 31.38404714475432 23.408034528552456 88-89 14.690699171460016 30.778080029902195 30.182112673131634 24.34910812550616 90-91 15.021022790946711 30.06487388400732 30.711537423888362 24.2025659011576 92-93 15.146169459444875 30.486180002407448 30.222190860828746 24.145459677318932 94-95 15.075481174326855 29.883239734186724 30.32819887016906 24.71308022131736 96-97 14.597473546126672 30.30208683595485 30.20411721483991 24.89632240307857 98-99 15.000083038546494 30.533688738312325 28.97090329330875 25.49532492983243 100-101 14.708628868589358 30.299609457681566 29.721474017107496 25.270287656621583 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2950.0 1 1789.5 2 652.5 3 640.0 4 490.0 5 476.0 6 768.5 7 1283.5 8 1715.5 9 1764.5 10 1369.5 11 928.5 12 796.5 13 774.0 14 719.5 15 710.0 16 719.0 17 687.5 18 728.0 19 811.0 20 844.5 21 909.0 22 1032.5 23 1115.5 24 1226.5 25 1428.0 26 1744.0 27 2120.5 28 2304.0 29 2468.0 30 2788.5 31 2981.0 32 3257.0 33 3645.0 34 3954.0 35 4093.0 36 4044.0 37 4126.5 38 3828.0 39 3325.5 40 3169.5 41 3113.0 42 3083.5 43 3002.5 44 3022.5 45 3056.5 46 3119.5 47 3739.0 48 4946.0 49 4983.0 50 4012.0 51 3035.0 52 1866.0 53 1121.5 54 778.0 55 588.5 56 488.5 57 442.0 58 439.0 59 419.0 60 378.0 61 324.5 62 234.0 63 144.0 64 83.0 65 59.5 66 52.5 67 43.5 68 31.0 69 23.0 70 24.5 71 21.0 72 11.0 73 9.5 74 13.0 75 10.5 76 10.0 77 11.5 78 8.5 79 7.0 80 9.0 81 8.5 82 3.5 83 2.0 84 2.0 85 1.5 86 3.0 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05062660801726284 3 0.0 4 0.0 5 0.05560627437961657 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0062245829529421525 26-27 0.0012449165905884306 28-29 0.0 30-31 0.002489833181176861 32-33 0.0 34-35 0.009959332724707445 36-37 0.02157855423686613 38-39 0.004564694165490912 40-41 4.149721968628102E-4 42-43 4.149721968628102E-4 44-45 0.008299443937256203 46-47 0.0 48-49 0.0 50-51 0.0016598887874512407 52-53 0.0 54-55 0.002489833181176861 56-57 0.0 58-59 0.01991866544941489 60-61 0.0 62-63 0.007469499543530584 64-65 0.012864138102747117 66-67 0.004979666362353722 68-69 0.012449165905884305 70-71 0.0 72-73 4.149721968628102E-4 74-75 0.02074860984314051 76-77 0.04938169142667441 78-79 0.027803137189808286 80-81 0.02489833181176861 82-83 0.036932525520790106 84-85 0.014109054693335548 86-87 0.008299443937256203 88-89 0.08091957838824798 90-91 0.02116358204000332 92-93 0.02531330400863142 94-95 0.024483359614905803 96-97 0.036932525520790106 98-99 0.0531164411984397 100-101 0.013694082496472736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 120490.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.24657647937588 #Duplication Level Percentage of deduplicated Percentage of total 1 79.38951265644492 32.74545605444435 2 9.652299891343716 7.9624865134036025 3 3.666143506780957 4.536476056104241 4 1.9397158839389916 3.2002655822059927 5 1.181134049659946 2.435886795584696 6 0.754557527465894 1.8673748858826458 7 0.5855366413135338 1.6905967300190887 8 0.3963942210954163 1.3079923645115776 9 0.29779870417320614 1.1054859324425264 >10 1.9115457362469315 14.113204415304176 >50 0.13078997142742163 3.7223006058594077 >100 0.06841321582357439 6.115030292970371 >500 0.012072920439454304 3.8028052120507922 >1k 0.014085073846030022 15.394638559216533 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4471 3.710681384347249 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3619 3.00356876089302 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3054 2.534650178438045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2441 2.025894265084239 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2135 1.7719312806041994 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1538 1.2764544775500042 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1291 1.071458212299776 RNA PCR Primer, Index 17 (95% over 21bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 977 0.8108556726699311 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 879 0.7295211220848202 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 850 0.7054527346667773 RNA PCR Primer, Index 17 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 663 0.5502531330400864 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 648 0.5378039671342021 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 565 0.46891858245497553 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 464 0.3850941986886879 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 454 0.37679475475143165 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.3452568677898581 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 307 0.2547929288737654 RNA PCR Primer, Index 17 (96% over 26bp) TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 297 0.24649348493650924 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 297 0.24649348493650924 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 275 0.2282347082745456 No Hit TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 271 0.2249149306996431 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 269 0.2232550419121919 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 263 0.21827537554983817 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 261 0.2166154867623869 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 253 0.20997593161258196 No Hit CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 252 0.20914598721885636 TruSeq Adapter, Index 12 (95% over 23bp) CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 251 0.20831604282513075 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 237 0.19669682131297203 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 230 0.1908872105568927 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 192 0.15934932359531911 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 191 0.1585193792015935 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 191 0.1585193792015935 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 180 0.14938999087061167 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 167 0.1386007137521786 No Hit TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT 156 0.12947132542119677 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 152 0.12615154784629432 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148 0.12283177027139182 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 143 0.11868204830276372 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 142 0.1178521039090381 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 128 0.1062328823968794 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 126 0.10457299360942818 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 125 0.10374304921570254 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.299443937256204E-4 0.0 18-19 0.0 0.0 0.0 0.002904805378039671 0.0 20-21 0.0 0.0 0.0 0.0074694995435305835 0.0 22-23 0.0 0.0 0.0 0.02655822059921985 0.0 24-25 0.0 0.0 0.0 0.057681135363930616 0.0 26-27 0.0 0.0 0.0 0.11121254875923313 0.0 28-29 0.0 0.0 0.0 0.2323844302431737 0.0 30-31 0.0 0.0 0.0 0.4813677483608598 0.0 32-33 0.0 0.0 0.0 0.7938418125985559 0.0 34-35 0.0 0.0 0.0 1.2370321188480373 0.0 36-37 0.0 0.0 0.0 1.757822225910864 0.0 38-39 0.0 0.0 0.0 2.3068304423603623 0.0 40-41 0.0 0.0 0.0 2.850858992447506 0.0 42-43 0.0 0.0 0.0 3.29736907627189 0.0 44-45 0.0 0.0 0.0 3.7401444103245085 0.0 46-47 0.0 0.0 0.0 4.268404016930866 0.0 48-49 0.0 0.0 0.0 4.743547182338784 0.0 50-51 0.0 0.0 0.0 5.184247655407088 0.0 52-53 0.0 0.0 0.0 5.595485102498133 0.0 54-55 0.0 0.0 0.0 6.0312059092040835 0.0 56-57 0.0 0.0 0.0 6.5843638476222095 0.0 58-59 0.0 0.0 0.0 7.098099427338369 0.0 60-61 0.0 0.0 0.0 7.620134450991784 0.0 62-63 0.0 0.0 0.0 8.119346003817745 0.0 64-65 0.0 0.0 0.0 8.643870860652335 0.0 66-67 0.0 0.0 0.0 9.224831936260271 0.0 68-69 0.0 0.0 0.0 9.84812017594821 0.0 70-71 0.0 0.0 0.0 10.496721719644784 0.0 72-73 0.0 0.0 0.0 11.084322350402523 0.0 74-75 0.0 0.0 0.0 11.742053282430078 0.0 76-77 0.0 0.0 0.0 12.363266661133704 0.0 78-79 0.0 0.0 0.0 13.09652253299029 0.0 80-81 0.0 0.0 0.0 13.844717403933936 0.0 82-83 0.0 0.0 0.0 14.514482529670513 0.0 84-85 0.0 0.0 0.0 15.149804963067474 0.0 86-87 0.0 0.0 0.0 15.802971200929537 0.0 88-89 0.0 0.0 0.0 16.498049630674743 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCAGG 20 0.0020726097 71.22353 7 AGTACAC 20 0.0020726097 71.22353 2 GTGGTAC 25 0.0050177705 56.978832 9 TGGGGCA 40 5.378716E-4 47.482357 6 ATGGGGC 70 4.2890115E-6 40.69916 5 GGTATCA 2635 0.0 27.750599 1 CCACGCA 35 0.008359886 27.155321 94-95 ATCCTAC 70 0.008377467 27.132776 6 ACTTTAT 90 8.9202635E-4 26.379087 8 AACTTGC 95 0.001222834 24.990715 7 TATCCTA 95 0.001222834 24.990715 5 ATGGGGT 135 1.3336263E-5 24.620481 5 GTATCAA 3525 0.0 24.24631 1 CATGGGG 490 0.0 23.256664 4 CTATCAC 125 2.2453038E-4 22.791533 2 GAGTACT 2285 0.0 22.546326 12-13 AGTACTT 2105 0.0 22.218584 12-13 GTACTTT 2260 0.0 21.64019 14-15 AACTTTA 115 0.0037122213 20.644505 7 TAACTTT 115 0.0037122213 20.644505 6 >>END_MODULE