##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938398_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 118519 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.019414608628153 31.0 31.0 33.0 26.0 34.0 2 29.962858275888255 31.0 31.0 34.0 25.0 34.0 3 29.91134754765059 31.0 30.0 34.0 25.0 34.0 4 33.47965305140948 37.0 35.0 37.0 28.0 37.0 5 33.534597828196326 37.0 35.0 37.0 28.0 37.0 6 33.67193445776626 37.0 35.0 37.0 29.0 37.0 7 33.61386781866199 37.0 35.0 37.0 28.0 37.0 8 33.61315063407555 37.0 35.0 37.0 29.0 37.0 9 34.91390409976459 39.0 35.0 39.0 28.0 39.0 10-11 34.80974358541668 38.5 35.0 39.0 27.5 39.0 12-13 34.670542275922 38.0 35.0 39.0 27.0 39.0 14-15 35.333068959407356 39.0 35.0 41.0 26.5 41.0 16-17 35.2459943131481 39.0 35.0 40.5 26.5 41.0 18-19 35.343894227929695 38.5 35.0 40.5 27.0 41.0 20-21 35.40633991174411 39.0 35.0 41.0 27.0 41.0 22-23 35.24998945316785 39.0 35.0 41.0 26.5 41.0 24-25 35.05661961373282 39.0 35.0 41.0 25.0 41.0 26-27 34.71175085851213 38.0 35.0 41.0 24.0 41.0 28-29 34.3974341666737 38.0 35.0 40.0 23.0 41.0 30-31 33.84705827757575 37.5 34.0 40.0 21.0 41.0 32-33 33.136311477484625 35.5 33.0 40.0 17.5 41.0 34-35 32.65062985681621 35.0 33.0 40.0 13.0 41.0 36-37 32.14171989301293 35.0 33.0 39.5 5.5 41.0 38-39 31.63750959761726 35.0 33.0 39.0 2.0 41.0 40-41 30.951902226647206 35.0 31.0 39.0 2.0 41.0 42-43 30.31268826095394 35.0 30.5 39.0 2.0 41.0 44-45 29.488284578843896 35.0 27.5 39.0 2.0 41.0 46-47 28.558408356466053 35.0 23.5 39.0 2.0 41.0 48-49 27.74843695947485 35.0 19.0 39.0 2.0 41.0 50-51 26.759722069879093 34.0 10.5 38.5 2.0 40.5 52-53 26.611045486377712 35.0 4.5 38.5 2.0 40.0 54-55 26.532361899779783 35.0 2.0 39.0 2.0 40.5 56-57 26.075776879656427 35.0 2.0 39.0 2.0 41.0 58-59 25.654861245876187 35.0 2.0 38.5 2.0 40.0 60-61 25.125342772044988 34.0 2.0 38.0 2.0 40.0 62-63 24.587327770230935 33.0 2.0 38.0 2.0 40.0 64-65 24.11025236459977 33.0 2.0 37.5 2.0 40.0 66-67 23.538837654722027 33.0 2.0 37.0 2.0 39.5 68-69 22.95748361022283 32.0 2.0 36.0 2.0 39.0 70-71 22.358301200651375 31.0 2.0 35.5 2.0 39.0 72-73 21.821674161948714 31.0 2.0 35.0 2.0 38.0 74-75 21.301500181405512 30.0 2.0 35.0 2.0 37.0 76-77 20.650798606130664 29.0 2.0 35.0 2.0 37.0 78-79 19.952176444283197 28.5 2.0 35.0 2.0 36.0 80-81 19.328512727917044 26.5 2.0 34.5 2.0 36.0 82-83 18.733025928332168 25.5 2.0 34.0 2.0 35.0 84-85 18.103122706064006 23.5 2.0 34.0 2.0 35.0 86-87 17.551894632928054 19.5 2.0 34.0 2.0 35.0 88-89 16.876496595482582 11.0 2.0 33.5 2.0 35.0 90-91 16.199946843965947 2.0 2.0 33.0 2.0 35.0 92-93 15.551493853306221 2.0 2.0 33.0 2.0 35.0 94-95 14.858107138939747 2.0 2.0 32.0 2.0 35.0 96-97 14.005032948303647 2.0 2.0 31.5 2.0 35.0 98-99 13.082585914494722 2.0 2.0 31.0 2.0 34.0 100-101 10.892143875665505 2.0 2.0 26.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0279196366808279 1101 2 0.9203342603506073 1101 3 0.9402020902393602 1101 4 0.42279641189124817 1101 5 0.8776826931115949 1101 6 1.1514981510606077 1101 7 0.7553511482823438 1101 8 1.0892602395935498 1101 9 1.3251803113659832 1101 10-11 1.010131238658225 1101 12-13 1.2046652516184722 1101 14-15 1.449801805561286 1101 16-17 1.597435893549168 1101 18-19 1.1954223732961324 1101 20-21 1.4249459698087819 1101 22-23 1.9407285727425432 1101 24-25 1.8610343939437897 1101 26-27 1.671602448767615 1101 28-29 0.9827982677311837 1101 30-31 1.2642982886778853 1101 32-33 1.6458112526507946 1101 34-35 2.0316166544405476 1101 36-37 1.7370788896414027 1101 38-39 1.3802224315736744 1101 40-41 1.8117669883514154 1101 42-43 1.3265451446435357 1101 44-45 2.204296463924017 1101 46-47 2.1334532065908824 1101 48-49 2.3417860932418755 1101 50-51 2.7435765290807943 1101 52-53 2.4367125743985234 1101 54-55 3.211381961699221 1101 56-57 3.4453674590673486 1101 58-59 3.1546229179403795 1101 60-61 4.028458029143309 1101 62-63 3.3355302179541617 1101 64-65 3.060282311283327 1101 66-67 3.2030932674275476 1101 68-69 3.2421830513901355 1101 70-71 3.6858696468181407 1101 72-73 3.8694232308576204 1101 74-75 3.599022513265325 1101 76-77 4.083063894505933 1101 78-79 4.370306120543422 1101 80-81 3.9643516738384044 1101 82-83 3.5084885775939583 1101 84-85 2.9814901055389065 1101 86-87 2.817931223785582 1101 88-89 2.8195129608799654 1101 90-91 3.155766581395941 1101 92-93 3.3957977699521553 1101 94-95 3.4443778554323696 1101 96-97 3.41290455705329 1101 98-99 3.055543427973314 1101 100-101 3.2981282545659756 1102 1 0.10474378141202934 1102 2 0.31251465990475324 1102 3 0.5182694828484671 1102 4 -0.8754115963399087 1102 5 -0.5990385621400591 1102 6 -0.9623662063088503 1102 7 -0.5257943545899835 1102 8 -0.4846058326005718 1102 9 -0.42514121825761464 1102 10-11 -0.6125644657060008 1102 12-13 -0.5114883966709911 1102 14-15 -0.15167294364561457 1102 16-17 -0.5164284638202901 1102 18-19 0.2682595506947436 1102 20-21 -0.10896468782647162 1102 22-23 -0.9412914907059715 1102 24-25 -0.5765888590785835 1102 26-27 -0.19712772357225816 1102 28-29 -1.2693666984010292 1102 30-31 -0.8111111257094947 1102 32-33 -0.534588301494793 1102 34-35 -0.5384339328869032 1102 36-37 -0.8420173977632146 1102 38-39 -0.9893222824793177 1102 40-41 0.1851700578875537 1102 42-43 0.0604900987719752 1102 44-45 0.6630549308707714 1102 46-47 -0.7446656307579964 1102 48-49 -0.8654933374363374 1102 50-51 -0.6106205042725463 1102 52-53 -0.9821333486058492 1102 54-55 -0.07743738152293744 1102 56-57 -0.8246316835203231 1102 58-59 -1.26893877287673 1102 60-61 -1.1270765674441883 1102 62-63 -1.086573871902651 1102 64-65 -0.9682266486755147 1102 66-67 -0.6603209484688755 1102 68-69 -0.9862315932124517 1102 70-71 -0.3496272236268574 1102 72-73 -0.20988740962938834 1102 74-75 -0.657986832529069 1102 76-77 -0.9665802793772862 1102 78-79 -0.2801697432995027 1102 80-81 0.00867989127817026 1102 82-83 0.3790621864424537 1102 84-85 -0.24568129118577886 1102 86-87 0.12445613161375846 1102 88-89 0.1925301948677891 1102 90-91 0.521838775514091 1102 92-93 0.19254817722301176 1102 94-95 0.40763773710946616 1102 96-97 0.04601782122202813 1102 98-99 -0.5078193431833355 1102 100-101 -0.24075539458089956 1103 1 0.866103755630526 1103 2 1.0766139186865757 1103 3 0.09980348220392798 1103 4 0.10968347294787861 1103 5 0.8305457111438628 1103 6 0.3208274772232187 1103 7 0.18456658643483337 1103 8 0.42040544680645553 1103 9 0.7001407669180253 1103 10-11 0.449351422144467 1103 12-13 0.49733371096677814 1103 14-15 0.6200801984749162 1103 16-17 0.25814453005660454 1103 18-19 0.9020816583325129 1103 20-21 1.3986489119157142 1103 22-23 0.9046640362034779 1103 24-25 1.3126715340893185 1103 26-27 1.6615977034338627 1103 28-29 1.977934300632171 1103 30-31 1.7038743721957559 1103 32-33 1.7713253304742622 1103 34-35 1.6974265247347518 1103 36-37 1.4601095761330143 1103 38-39 1.8103473920292252 1103 40-41 1.877806216443787 1103 42-43 2.0949728573926656 1103 44-45 2.4883046891691976 1103 46-47 1.3843627288939508 1103 48-49 1.2545114965952422 1103 50-51 0.9991522962430821 1103 52-53 0.5260039378910868 1103 54-55 0.025285789601777964 1103 56-57 0.011011242680126543 1103 58-59 0.060863031828390035 1103 60-61 0.02628308450553618 1103 62-63 -0.056038905740873446 1103 64-65 -0.09552281367712467 1103 66-67 -0.3973496303895985 1103 68-69 -0.1065989796127127 1103 70-71 -1.0960822671331414 1103 72-73 -0.5340092272252654 1103 74-75 -0.7156326591484685 1103 76-77 -0.33424866481074034 1103 78-79 -0.13095930179128246 1103 80-81 0.010895489086742316 1103 82-83 0.2517660214408437 1103 84-85 0.2256770716888603 1103 86-87 -0.05097893122865216 1103 88-89 0.4363668046003042 1103 90-91 -0.14461304530447094 1103 92-93 0.49809925682339795 1103 94-95 0.4573879788110471 1103 96-97 1.0025518205774908 1103 98-99 0.6609689262333571 1103 100-101 0.7921965873720502 1104 1 0.42675646499013453 1104 2 0.3583257413466754 1104 3 0.1296680142236326 1104 4 0.17156770940107435 1104 5 -0.1266587223536746 1104 6 0.14502698461237884 1104 7 -0.6408644480479282 1104 8 0.014309387693153042 1104 9 -0.28261785377163307 1104 10-11 0.06988713643017519 1104 12-13 0.06352829224757528 1104 14-15 0.07959374527295182 1104 16-17 -0.08314241575620684 1104 18-19 0.06202623961330289 1104 20-21 -0.05994098956211502 1104 22-23 0.3654091100950936 1104 24-25 0.2917355734981797 1104 26-27 0.6799166689511011 1104 28-29 0.12094538437601443 1104 30-31 -0.4066551844544861 1104 32-33 -0.5510268862745065 1104 34-35 -0.1224626378268141 1104 36-37 -0.6875197342118113 1104 38-39 -0.49199250944861106 1104 40-41 -0.18315437468921303 1104 42-43 -0.3680813297994554 1104 44-45 -0.41517437487021525 1104 46-47 -0.293438995243978 1104 48-49 -0.43037150833086457 1104 50-51 -0.4229536150869677 1104 52-53 0.06818374084675227 1104 54-55 0.5382168240845395 1104 56-57 0.8590715875077137 1104 58-59 0.8687448052274078 1104 60-61 1.0950946608602194 1104 62-63 0.8109561681507529 1104 64-65 0.6837259547957792 1104 66-67 0.9448609607434051 1104 68-69 0.636473680485814 1104 70-71 0.42423792991119313 1104 72-73 0.7241496397698093 1104 74-75 0.2686653704081827 1104 76-77 -0.01990137417034532 1104 78-79 -0.15512802100310807 1104 80-81 -0.48310081633197655 1104 82-83 -0.5404137815148218 1104 84-85 -0.753233026833307 1104 86-87 -0.6034727735931824 1104 88-89 -0.08333762889722962 1104 90-91 -0.504988661068019 1104 92-93 -1.212491876181529 1104 94-95 -1.0275258142924013 1104 96-97 -1.338119361688518 1104 98-99 -1.3134768865745237 1104 100-101 -0.12159651607622557 1105 1 0.05342111390054427 1105 2 0.396310002847887 1105 3 0.5813931958216969 1105 4 0.2667553607327946 1105 5 0.06871052704099867 1105 6 0.31951245676734175 1105 7 0.4407033485622378 1105 8 0.13007815282632862 1105 9 0.0860262140249759 1105 10-11 0.002317523839380442 1105 12-13 0.10786848595217435 1105 14-15 0.7134466508419521 1105 16-17 0.1552807077304763 1105 18-19 0.31981982314840707 1105 20-21 -0.006596558569377464 1105 22-23 0.13303925537355354 1105 24-25 0.206081820471546 1105 26-27 0.21222599098499728 1105 28-29 -0.3145116374157553 1105 30-31 0.34954941602976675 1105 32-33 0.19972977232114175 1105 34-35 0.07593324595042361 1105 36-37 0.8882217912119117 1105 38-39 0.6169663283237874 1105 40-41 0.6397875139306421 1105 42-43 0.6579477258906188 1105 44-45 0.6776676348150161 1105 46-47 0.566200835264496 1105 48-49 1.1897102107974646 1105 50-51 1.0278635761963244 1105 52-53 1.29412199750535 1105 54-55 0.9852507223896261 1105 56-57 1.5471012485246618 1105 58-59 2.1414445630966554 1105 60-61 2.0783725058965388 1105 62-63 2.007082076141071 1105 64-65 2.3778845504374253 1105 66-67 2.3321944958523666 1105 68-69 2.610671622374433 1105 70-71 2.7290200122356474 1105 72-73 2.612542496912667 1105 74-75 2.4168191234348164 1105 76-77 2.9678407565802623 1105 78-79 2.988561446309241 1105 80-81 2.7326225492854554 1105 82-83 2.7378852499621757 1105 84-85 3.2919394913264988 1105 86-87 4.10952662107994 1105 88-89 3.820718061175409 1105 90-91 3.7997177553968733 1105 92-93 4.121345896215564 1105 94-95 3.596414863673699 1105 96-97 2.748972042669836 1105 98-99 1.8522313458709956 1105 100-101 1.4419628059576723 1106 1 0.5279973850869837 1106 2 0.9471574604750046 1106 3 0.9542745517539011 1106 4 1.1057384115802549 1106 5 0.9924393406003205 1106 6 0.9296819482927674 1106 7 0.87290673839275 1106 8 0.42668832231785103 1106 9 0.4250092648724362 1106 10-11 0.7226565068902318 1106 12-13 0.8070210283250532 1106 14-15 0.9846330915199175 1106 16-17 1.0662976568830231 1106 18-19 0.4596503316229814 1106 20-21 0.6501831024475635 1106 22-23 1.361852814695581 1106 24-25 1.2654038543698576 1106 26-27 1.216463279120596 1106 28-29 0.9227764981774698 1106 30-31 1.1037867041653655 1106 32-33 1.013289094355038 1106 34-35 1.0123739239165204 1106 36-37 1.21449297765259 1106 38-39 0.6678137859509086 1106 40-41 0.7923298868119986 1106 42-43 1.0138799292804492 1106 44-45 0.8937693297302687 1106 46-47 0.43484490306110857 1106 48-49 0.554116990458482 1106 50-51 0.27786357619632795 1106 52-53 0.4339525059799243 1106 54-55 0.9598269935760619 1106 56-57 0.34794870615178297 1106 58-59 -0.37126730131012664 1106 60-61 1.1970165736931477 1106 62-63 2.384200720208863 1106 64-65 2.2422913300984426 1106 66-67 1.806770767038806 1106 68-69 1.0386377240693463 1106 70-71 1.6485115376593775 1106 72-73 2.3286441918279195 1106 74-75 2.4507174285195603 1106 76-77 2.5398746548853453 1106 78-79 2.8317817852922893 1106 80-81 2.4444869560651163 1106 82-83 1.9878852499621757 1106 84-85 2.3724479659027686 1106 86-87 2.876475773622314 1106 88-89 1.9690231459211702 1106 90-91 0.5115821621765342 1106 92-93 0.36710860807997214 1106 94-95 1.2192962196059032 1106 96-97 1.0032093308054275 1106 98-99 1.653078803498115 1106 100-101 1.2300983991780114 1107 1 0.9591502076111489 1107 2 1.1122366032120823 1107 3 1.071447825321627 1107 4 0.7901479927540862 1107 5 1.2252450556864645 1107 6 0.7391090389217041 1107 7 1.114817370133892 1107 8 1.249772787929281 1107 9 1.438456526393658 1107 10-11 1.3842687775163682 1107 12-13 1.421777997088185 1107 14-15 1.0336595658160448 1107 16-17 1.3999228144681197 1107 18-19 1.2387160270985405 1107 20-21 1.385860368171059 1107 22-23 1.2031821325272105 1107 24-25 1.1912338025418663 1107 26-27 1.330484850769281 1107 28-29 1.6223842863830953 1107 30-31 2.197462288981164 1107 32-33 1.5699146721670516 1107 34-35 1.7751908450539347 1107 36-37 1.8344327451270317 1107 38-39 2.307889426796965 1107 40-41 2.764454834283633 1107 42-43 3.258837206209993 1107 44-45 3.7289003004544554 1107 46-47 3.9165300130204876 1107 48-49 3.858921584939498 1107 50-51 3.6352665738150414 1107 52-53 4.001994248520898 1107 54-55 3.7809854191258587 1107 56-57 3.576272000730853 1107 58-59 3.5465783353336633 1107 60-61 3.0726994424422713 1107 62-63 2.6577684467812155 1107 64-65 2.893362908750916 1107 66-67 2.5362496856548553 1107 68-69 2.8136062070501566 1107 70-71 2.22958031479903 1107 72-73 2.2613028274911784 1107 74-75 1.892410943017392 1107 76-77 2.0943990980846756 1107 78-79 1.924897067544709 1107 80-81 2.2250234457695584 1107 82-83 1.7943707521333465 1107 84-85 1.9512755327888875 1107 86-87 2.1171957624162623 1107 88-89 1.0717686284817525 1107 90-91 1.2217306423558298 1107 92-93 1.5857666834406654 1107 94-95 1.0979907146332177 1107 96-97 1.2686176512984932 1107 98-99 0.6644599493168588 1107 100-101 -0.08234031772911798 1108 1 0.4058278935615611 1108 2 -0.053317115796183145 1108 3 -0.002403414347792676 1108 4 0.10492485225822179 1108 5 -0.025730150925106443 1108 6 0.10724127032666075 1108 7 -0.359364448047927 1108 8 -0.20490489802113387 1108 9 -0.17661785377163142 1108 10-11 -0.041648577855532665 1108 12-13 -0.01111456489528706 1108 14-15 0.25270088813008584 1108 16-17 0.5811790128152197 1108 18-19 0.31663338247044237 1108 20-21 0.5571661532950216 1108 22-23 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-2.249929137258494 2316 92-93 -1.8053490190386707 2316 94-95 -1.2816924809590677 2316 96-97 -1.0669884093075677 2316 98-99 -1.487881648479286 2316 100-101 -0.6549298494095588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4213.0 3 711.0 4 541.0 5 450.0 6 453.0 7 486.0 8 631.0 9 701.0 10 898.0 11 1199.0 12 1641.0 13 2172.0 14 2543.0 15 3434.0 16 3804.0 17 4225.0 18 3505.0 19 2598.0 20 2513.0 21 2499.0 22 2291.0 23 2229.0 24 2407.0 25 2448.0 26 2698.0 27 2947.0 28 3254.0 29 3422.0 30 3905.0 31 4281.0 32 4709.0 33 5444.0 34 7072.0 35 9379.0 36 8209.0 37 9231.0 38 5071.0 39 305.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.558091970149 23.092265954479764 16.453545862526518 13.89609621284472 2 15.537939418498633 27.06834836051734 37.87779015972715 19.51592206125688 3 20.208876843713856 33.68033568889677 31.784909196828853 14.325878270560521 4 11.753829007582025 22.893834748982588 36.860635944544825 28.49170029889056 5 9.090755564562434 43.364744823859226 32.49907116560273 15.045428445975615 6 22.61139773869135 41.337701706536514 17.463078520945395 18.587822033826747 7 19.6285666047611 42.815296302549385 22.045416174236028 15.510720918453485 8 25.238456993331642 33.21093947834895 20.058242593061536 21.49236093525787 9 26.12428558162309 20.678243691590758 22.65624340455708 30.54122732222907 10-11 22.16650415563913 32.25527316626356 24.64342547919648 20.934797198900835 12-13 26.44393322217767 32.60927375952386 26.392857896625227 14.553935121673245 14-15 22.65465734171285 32.189239723627956 30.546539651453823 14.609563283205377 16-17 14.953361752416324 35.71350187819187 30.878740556282448 18.454395813109358 18-19 14.038479396891596 32.29719804479414 38.83396790288131 14.830354655432956 20-21 16.67736107124162 30.38195910234545 38.79198257374918 14.14869725266375 22-23 15.842595602233919 31.023288995453647 39.05265246334843 14.081462938964004 24-25 15.574057732985997 32.23403940907498 38.863579749122856 13.328323108816164 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32.30635511337266 36.9834518957326 16.76160097212729 52-53 14.47854210355318 32.93811366292685 35.88749319617383 16.69585103734615 54-55 14.433150635224026 31.608460652396804 36.040893997122396 17.91749471525677 56-57 15.202568711340641 31.479793760495518 35.49825742808199 17.819380100081855 58-59 15.697890295358649 30.554852320675106 35.730379746835446 18.0168776371308 60-61 15.16283112652641 30.995299459058035 35.940994286775194 17.90087512764036 62-63 14.82689370606948 30.73723114310064 35.88358886516863 18.552286285661246 64-65 14.897369364808135 31.35160221926882 35.615889289707404 18.13513912621564 66-67 15.36975158633725 30.387640070203858 34.689314162278926 19.553294181179965 68-69 15.136159188530055 30.131391296275982 35.191264208740854 19.54118530645311 70-71 16.196963174908632 30.194380438727535 34.62807754960795 18.980578836755882 72-73 16.160068868605286 28.779228013317976 35.6546948386525 19.40600827942424 74-75 15.718113725076485 30.181664732566727 34.53402257622112 19.566198966135666 76-77 16.185846719917635 28.8593237717888 34.81440223468402 20.140427273609546 78-79 16.41451004548101 29.046670773177176 34.50228248854537 20.03653669279645 80-81 17.157563867265786 28.766533763685842 33.51770984958758 20.558192519460793 82-83 17.365842956412408 28.63887400481822 33.430513423087795 20.564769615681573 84-85 17.526347320547114 28.61964510222507 33.01199024579582 20.842017331431993 86-87 17.95655053984836 28.647615511516346 32.51874385577041 20.87709009286489 88-89 19.02309935720225 28.330371877281763 31.577281341138637 21.069247424377355 90-91 19.498482616568392 27.852744163666372 31.589432764508 21.05934045525724 92-93 20.577896127243495 28.133084067976853 31.118360250334444 20.17065955444521 94-95 20.691503505210335 27.452908628177585 31.0771399509566 20.77844791565548 96-97 20.766750238475108 27.1950262111581 30.77384117979757 21.26438237056922 98-99 21.492806893903268 27.414828475331593 29.618970050176607 21.473394580588533 100-101 22.314978327179322 26.289596201130532 30.366029860330713 21.02939561135943 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3021.0 1 1807.0 2 634.5 3 658.5 4 503.5 5 457.0 6 716.5 7 1228.0 8 1702.0 9 1782.0 10 1401.5 11 963.0 12 825.5 13 773.5 14 736.0 15 710.5 16 689.0 17 717.5 18 758.0 19 787.5 20 878.0 21 975.0 22 1075.5 23 1154.5 24 1218.0 25 1381.0 26 1653.5 27 2001.5 28 2231.0 29 2413.5 30 2646.5 31 2853.5 32 3115.0 33 3444.5 34 3818.5 35 4039.5 36 4026.5 37 4047.5 38 3796.5 39 3311.0 40 3092.5 41 2979.0 42 2776.0 43 2599.0 44 2515.0 45 2496.5 46 2564.5 47 3069.5 48 4177.0 49 4350.0 50 3386.0 51 2578.0 52 1950.5 53 1528.5 54 1271.5 55 1008.5 56 820.5 57 722.0 58 713.5 59 670.5 60 583.0 61 523.0 62 458.0 63 359.0 64 274.0 65 251.5 66 226.5 67 187.0 68 152.0 69 133.0 70 113.0 71 97.5 72 90.0 73 71.5 74 49.5 75 43.0 76 40.0 77 32.0 78 29.0 79 21.5 80 17.0 81 15.0 82 13.5 83 9.0 84 5.0 85 5.5 86 4.5 87 3.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16706182131135092 2 0.05653102034272986 3 0.0641247394932458 4 0.06834347235464355 5 0.07678093807743906 6 0.07762468464971861 7 0.04978104776449346 8 0.041343582041697954 9 0.05568727377045031 10-11 0.05526540048431053 12-13 0.05610914705659008 14-15 0.04682793476151503 16-17 0.04556231490309571 18-19 0.05568727377045031 20-21 0.06496848606552535 22-23 0.06117162649026738 24-25 0.07973405108041749 26-27 0.06623410592394469 28-29 0.059062260059568515 30-31 0.06749972578236402 32-33 0.06285911963482649 34-35 0.06074975320412761 36-37 0.059062260059568515 38-39 0.03712484918030021 40-41 0.0354373560357411 42-43 0.02995300331592403 44-45 0.0067499725782364005 46-47 0.013921818442612578 48-49 0.013078071870333026 50-51 0.013499945156472801 52-53 0.016453058159451226 54-55 0.014765565014892129 56-57 0.013499945156472801 58-59 0.01603118487331145 60-61 0.018562424590150103 62-63 0.0067499725782364005 64-65 0.009703085581214826 66-67 0.005906226005956851 68-69 0.015187438301031902 70-71 0.03374986289118201 72-73 0.027421763599085377 74-75 0.02657801702680583 76-77 0.019828044448569428 78-79 0.0067499725782364005 80-81 0.009703085581214826 82-83 0.008859339008935275 84-85 0.005062479433677301 86-87 0.011390578725773927 88-89 0.044296695044676385 90-91 0.04935917447835369 92-93 0.03164049646048313 94-95 0.044296695044676385 96-97 0.0489373011922139 98-99 0.03164049646048313 100-101 0.1417494241429644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 118519.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.50614669377906 #Duplication Level Percentage of deduplicated Percentage of total 1 83.36551340256398 40.43739822306972 2 7.744090173772373 7.512719479577114 3 3.031884360486354 4.411950826449767 4 1.5481222494738123 3.0037377973151984 5 0.9862756353389344 2.3920215324125245 6 0.6383829950077407 1.8579299521595694 7 0.43486580041399225 1.4765565014892128 8 0.3322374715162901 1.2892447624431524 9 0.25048270103845954 1.0934955576742968 >10 1.5046356694324132 12.950666137918814 >50 0.09740993929273427 3.1716433651988294 >100 0.048704969646367134 4.971354803871109 >500 0.008697316008279846 3.092331187404551 >1k 0.008697316008279846 12.33894987301614 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4097 3.456829706629317 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3596 3.0341126739172624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2952 2.490739881369232 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2296 1.9372421299538471 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1683 1.4200254811464827 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 949 0.800715497093293 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.6724660181068014 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 693 0.5847163745897282 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 638 0.538310313114353 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.49612298450037545 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 468 0.3948733958268295 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 458 0.386435930104034 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 408 0.3442486014900564 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 306 0.25818645111754235 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 300 0.253123971683865 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 279 0.23540529366599447 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 262 0.2210616019372421 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 244 0.20587416363621022 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 242 0.20418667049165112 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 241 0.2033429239193716 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 233 0.19659295134113516 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 217 0.18309300618466237 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 198 0.16706182131135092 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 166 0.14006193099840533 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164 0.13837443785384623 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 161 0.13584319813700757 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 150 0.1265619858419325 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 149 0.12571823926965298 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 144 0.12149950640825521 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 141 0.11896826669141657 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.11728077354685745 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.11728077354685745 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 129 0.10884330782406197 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 124 0.10462457496266421 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.2187328613977503E-4 0.0 18-19 0.0 0.0 0.0 0.0012656198584193252 0.0 20-21 0.0 0.0 0.0 0.007171845864376176 0.0 22-23 0.0 0.0 0.0 0.030374876602063804 0.0 24-25 0.0 0.0 0.0 0.07340595178832085 0.0 26-27 0.0 0.0 0.0 0.12951509884491094 0.0 28-29 0.0 0.0 0.0 0.22696782794319897 0.0 30-31 0.0 0.0 0.0 0.44085758401606495 0.0 32-33 0.0 0.0 0.0 0.7374345041723268 0.0 34-35 0.0 0.0 0.0 1.1525578177338653 0.0 36-37 0.0 0.0 0.0 1.6735713261164875 0.0 38-39 0.0 0.0 0.0 2.2435221356913235 0.0 40-41 0.0 0.0 0.0 2.8071448459740633 0.0 42-43 0.0 0.0 0.0 3.3100178030526752 0.0 44-45 0.0 0.0 0.0 3.799390814974814 0.0 46-47 0.0 0.0 0.0 4.359216665682296 0.0 48-49 0.0 0.0 0.0 4.863777115905467 0.0 50-51 0.0 0.0 0.0 5.3599001004058415 0.0 52-53 0.0 0.0 0.0 5.815523249436799 0.0 54-55 0.0 0.0 0.0 6.304474388072799 0.0 56-57 0.0 0.0 0.0 6.875268944219915 0.0 58-59 0.0 0.0 0.0 7.382782507446064 0.0 60-61 0.0 0.0 0.0 7.904639762400965 0.0 62-63 0.0 0.0 0.0 8.40160649347362 0.0 64-65 0.0 0.0 0.0 8.907010690269072 0.0 66-67 0.0 0.0 0.0 9.475274006699347 0.0 68-69 0.0 0.0 0.0 10.09036525789114 0.0 70-71 0.0 0.0 0.0 10.741315738404813 0.0 72-73 0.0 0.0 0.0 11.325610239708402 0.0 74-75 0.0 0.0 0.0 11.961373281921043 0.0 76-77 0.0 0.0 0.0 12.570136433820739 0.0 78-79 0.0 0.0 0.0 13.26116487651769 0.0 80-81 0.0 0.0 0.0 13.95345893907306 0.0 82-83 0.0 0.0 0.0 14.614112505167949 0.0 84-85 0.0 0.0 0.0 15.276453564407394 0.0 86-87 0.0 0.0 0.0 15.925716551776508 0.0 88-89 0.0 0.0 0.0 16.555573367983193 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 50 0.0015894995 38.099026 1 AGGCCGA 50 0.0016061051 38.01858 6 ACTGATA 50 0.0016094426 38.002533 8 ACCCATG 55 0.002564175 34.562347 3 ATTAGCC 60 0.003927369 31.682152 3 ATGGGGA 260 0.0 29.245062 5 TACCCAT 65 0.005809306 29.245062 2 GGCCGAC 65 0.0058213137 29.232718 7 ATGGGTA 85 6.3548406E-4 27.954838 5 CATGGGG 460 0.0 27.894066 4 CCTGGCT 35 0.008392906 27.133215 50-51 GGTATCA 2525 0.0 25.273613 1 GTATCAA 3350 0.0 23.882973 1 ACGAAGG 50 0.0016430747 23.766632 94-95 GTACATG 1440 0.0 23.15045 1 TACATGG 1445 0.0 22.363873 2 ACATGGG 1400 0.0 22.064354 3 GAGTACT 2105 0.0 22.002655 12-13 ATGGGGT 130 2.9103566E-4 21.933796 5 AGTACTT 2010 0.0 21.860909 12-13 >>END_MODULE