##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938398_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 118519 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.615909685366905 31.0 31.0 34.0 30.0 34.0 2 31.790430226377204 31.0 31.0 34.0 30.0 34.0 3 31.78200119812013 31.0 31.0 34.0 30.0 34.0 4 35.51682852538411 37.0 35.0 37.0 33.0 37.0 5 35.43478260869565 37.0 35.0 37.0 33.0 37.0 6 35.51433947299589 37.0 35.0 37.0 33.0 37.0 7 35.45496502670458 37.0 35.0 37.0 33.0 37.0 8 35.50773293733494 37.0 35.0 37.0 33.0 37.0 9 36.92256093959618 39.0 37.0 39.0 32.0 39.0 10-11 36.89746369780373 39.0 37.0 39.0 32.0 39.0 12-13 36.79748394772146 39.0 37.0 39.0 32.0 39.0 14-15 37.54732996397202 39.5 36.5 41.0 32.0 41.0 16-17 37.53170799618626 39.0 36.5 41.0 32.0 41.0 18-19 37.7075152507193 39.0 36.5 41.0 33.0 41.0 20-21 37.78365916013466 40.0 36.0 41.0 33.0 41.0 22-23 37.60436723225812 39.5 36.0 41.0 32.5 41.0 24-25 37.39961103283018 39.0 35.5 41.0 32.0 41.0 26-27 37.03746656654208 39.0 35.0 41.0 31.5 41.0 28-29 36.75607286595398 39.0 35.0 41.0 31.0 41.0 30-31 36.12022123035125 38.0 35.0 40.0 30.5 41.0 32-33 35.42916325652427 37.0 35.0 40.0 30.0 41.0 34-35 34.84160767471882 36.0 34.0 40.0 28.5 41.0 36-37 34.35912385355934 36.0 33.5 40.0 24.5 41.0 38-39 33.87807018283988 35.0 33.0 40.0 23.5 41.0 40-41 33.47032965178579 35.0 33.0 40.0 21.0 41.0 42-43 32.86846412811448 35.0 33.0 40.0 15.0 41.0 44-45 32.1111340797678 35.0 33.0 40.0 2.0 41.0 46-47 31.139585214185068 35.0 31.5 40.0 2.0 41.0 48-49 30.310232958428607 35.0 30.5 40.0 2.0 41.0 50-51 29.620723259561757 35.0 27.5 39.5 2.0 41.0 52-53 29.014984939123686 35.0 26.5 39.0 2.0 41.0 54-55 28.50618044364195 35.0 23.5 39.0 2.0 41.0 56-57 27.86517351648259 35.0 21.0 39.0 2.0 40.0 58-59 27.14101114589222 35.0 15.5 38.0 2.0 40.0 60-61 26.410368801626745 34.0 4.5 38.0 2.0 40.0 62-63 25.70859524633181 33.5 2.0 38.0 2.0 40.0 64-65 25.015803373298795 33.0 2.0 37.0 2.0 40.0 66-67 24.263788928357478 33.0 2.0 37.0 2.0 39.5 68-69 23.57826593204465 32.5 2.0 36.0 2.0 39.0 70-71 22.967397632447117 31.5 2.0 35.5 2.0 39.0 72-73 22.55645930188409 31.0 2.0 35.0 2.0 38.0 74-75 22.175739754807246 31.0 2.0 35.0 2.0 37.0 76-77 21.077126874172073 29.0 2.0 34.0 2.0 36.0 78-79 21.504421232038744 30.0 2.0 35.0 2.0 36.0 80-81 21.19215906310381 30.0 2.0 35.0 2.0 36.0 82-83 20.77282545414659 29.5 2.0 35.0 2.0 35.5 84-85 20.351192635779917 29.0 2.0 34.0 2.0 35.0 86-87 19.919232359368543 29.0 2.0 34.0 2.0 35.0 88-89 19.395696048734802 27.0 2.0 34.0 2.0 35.0 90-91 18.99459580320455 26.5 2.0 34.0 2.0 35.0 92-93 18.466051856664333 25.0 2.0 34.0 2.0 35.0 94-95 17.920704697137168 23.5 2.0 34.0 2.0 35.0 96-97 17.227596419139548 19.0 2.0 33.0 2.0 35.0 98-99 16.44989410980518 2.0 2.0 33.0 2.0 35.0 100-101 14.11122267315789 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22732873400570242 1101 2 0.03151119497212562 1101 3 0.5306559827856141 1101 4 0.2654891907798742 1101 5 -0.06986358798457815 1101 6 0.3949856602831545 1101 7 0.25686709067631597 1101 8 0.03973115568043539 1101 9 0.6363735578665626 1101 10-11 0.6238581624430779 1101 12-13 0.5133967593426831 1101 14-15 0.5885475551406287 1101 16-17 0.6631937981556177 1101 18-19 0.5897376461538144 1101 20-21 0.7223954622919848 1101 22-23 0.3734403417559662 1101 24-25 0.38853802631625456 1101 26-27 0.6841417416515583 1101 28-29 0.2984754588764815 1101 30-31 0.9331798856757203 1101 32-33 1.5825954079691158 1101 34-35 1.5748362333117285 1101 36-37 1.7378442167671153 1101 38-39 1.8874094770481662 1101 40-41 1.7021906495605776 1101 42-43 1.4837941521700841 1101 44-45 1.0029317443026855 1101 46-47 1.7824131675199837 1101 48-49 1.4044444403560412 1101 50-51 1.029430804455913 1101 52-53 1.1992045757533418 1101 54-55 0.72769400019099 1101 56-57 0.3984407313537943 1101 58-59 0.5483038764606327 1101 60-61 0.44109229283010265 1101 62-63 0.40302006510292543 1101 64-65 0.5572029002600196 1101 66-67 1.1024557588086772 1101 68-69 1.124193312326362 1101 70-71 0.7768476676189344 1101 72-73 0.7769634423680749 1101 74-75 1.3087722083378672 1101 76-77 1.4893311144719803 1101 78-79 1.0867109616084498 1101 80-81 0.8274079447815623 1101 82-83 1.0382579811317463 1101 84-85 0.5930048950894253 1101 86-87 0.8881818859809734 1101 88-89 0.7076379416989127 1101 90-91 0.6321550665432305 1101 92-93 0.9444725392910165 1101 94-95 1.165326332141703 1101 96-97 1.0035175309520845 1101 98-99 1.7228664424232427 1101 100-101 0.6599029496595001 1102 1 0.28134571076982695 1102 2 0.19682029211694285 1102 3 0.30318449174557927 1102 4 -0.0653862272086414 1102 5 -0.20267675783796335 1102 6 0.0014334010016696652 1102 7 -0.25277879803156367 1102 8 0.18206160239225966 1102 9 0.46060403030075747 1102 10-11 0.10619546845353511 1102 12-13 0.2957855100929194 1102 14-15 0.5918914632260268 1102 16-17 0.5329099946745188 1102 18-19 0.6383957958580027 1102 20-21 0.5735486818752804 1102 22-23 1.1542428796964614 1102 24-25 0.9416118495178338 1102 26-27 1.0021988453127122 1102 28-29 1.2066465983774748 1102 30-31 0.8893661156336989 1102 32-33 0.734014425374589 1102 34-35 0.9107703840448167 1102 36-37 0.6138195232920225 1102 38-39 0.6364877587938551 1102 40-41 0.7391879915823552 1102 42-43 0.4542991680322146 1102 44-45 -0.32991572204387865 1102 46-47 -0.812073671200757 1102 48-49 -0.4085968011770156 1102 50-51 -0.5130968470917168 1102 52-53 -1.360642804851988 1102 54-55 -1.3558608313274867 1102 56-57 -1.337947847913977 1102 58-59 -1.699916992955469 1102 60-61 -0.9719232263330646 1102 62-63 -0.2582163234763435 1102 64-65 -0.8749757844694663 1102 66-67 -0.19296565935131227 1102 68-69 0.17070793120658223 1102 70-71 -0.8939488684352526 1102 72-73 -0.3270406732111084 1102 74-75 -0.46452797171872007 1102 76-77 -0.42762850140729824 1102 78-79 -0.6201397629049694 1102 80-81 -0.4361177347177083 1102 82-83 -0.6264080567658787 1102 84-85 -0.986262874781108 1102 86-87 -0.7862586524739719 1102 88-89 -0.6802553962072828 1102 90-91 -0.49950059666521085 1102 92-93 -0.10319958623415104 1102 94-95 0.17784455215370443 1102 96-97 -0.22515433734938384 1102 98-99 -0.21737191820438184 1102 100-101 -0.5912759922936832 1103 1 0.0874204771249687 1103 2 -0.02804274365488979 1103 3 0.2693462706691996 1103 4 -0.1361242226968784 1103 5 0.1735720336541462 1103 6 -0.06720984746755221 1103 7 0.22981868827201168 1103 8 -0.1352925709883408 1103 9 -0.07202568288003164 1103 10-11 -0.030325640151041 1103 12-13 -0.3056083023466627 1103 14-15 -0.12483428604886626 1103 16-17 -0.11662271560584969 1103 18-19 -0.13847336302050195 1103 20-21 0.07362924906831836 1103 22-23 0.2729183550428971 1103 24-25 0.2904677953766779 1103 26-27 0.49998324750414014 1103 28-29 0.1035608748840815 1103 30-31 0.14359589326824818 1103 32-33 0.4040917698799049 1103 34-35 0.4127039966777488 1103 36-37 0.4205791107879904 1103 38-39 -0.2658648032428843 1103 40-41 -0.09182232101835552 1103 42-43 -0.06322419645376698 1103 44-45 -0.02593570270775558 1103 46-47 -0.10650647816176217 1103 48-49 -0.8047456893497511 1103 50-51 -0.8375812170562646 1103 52-53 -0.36620194117168126 1103 54-55 -0.2374673411566839 1103 56-57 -0.049597864027415284 1103 58-59 -0.33136236839859023 1103 60-61 -0.8289567422853672 1103 62-63 -0.910488318320045 1103 64-65 -0.6315822942986635 1103 66-67 -0.5881880248041007 1103 68-69 -0.42037328052400014 1103 70-71 -0.13726179141301742 1103 72-73 -0.3657129258698255 1103 74-75 -0.2593233310484031 1103 76-77 -0.18608805667639317 1103 78-79 0.26529368859358016 1103 80-81 0.3398249014408492 1103 82-83 0.28323583624088755 1103 84-85 0.20452023833523114 1103 86-87 0.32419896918249336 1103 88-89 0.02458669209435982 1103 90-91 -0.3587497104260855 1103 92-93 0.3914829790252732 1103 94-95 0.30163604425812096 1103 96-97 0.8150486919770756 1103 98-99 0.7583773566908789 1103 100-101 0.3221142751893957 1104 1 -0.135793808589316 1104 2 -0.18752550227558018 1104 3 -0.3978335322864659 1104 4 0.038752624593762164 1104 5 -0.33043042940004597 1104 6 0.0036029604142200355 1104 7 0.04970785083359175 1104 8 0.0946951137407197 1104 9 0.5668289969229221 1104 10-11 -0.19011628054512641 1104 12-13 -0.11225854865207197 1104 14-15 -0.15285152742817587 1104 16-17 -0.005015572748703789 1104 18-19 -0.15633050587764075 1104 20-21 -0.1625160711287279 1104 22-23 -0.46645972377483247 1104 24-25 -0.8125186578252865 1104 26-27 -0.6265561613628634 1104 28-29 -0.16814479014055195 1104 30-31 4.3086863770724904E-4 1104 32-33 -0.11207202322353993 1104 34-35 -0.7235336880513188 1104 36-37 -0.8098703966011698 1104 38-39 -0.20613573920346795 1104 40-41 -0.6610957200331313 1104 42-43 0.012668660689094224 1104 44-45 0.046878336701112744 1104 46-47 -0.4795052466346661 1104 48-49 0.3128651480886724 1104 50-51 1.1707315908255111 1104 52-53 0.6197894381386639 1104 54-55 0.7469846292866684 1104 56-57 0.2907654364651968 1104 58-59 0.35937039022210016 1104 60-61 0.7963511394880314 1104 62-63 0.8997025683794639 1104 64-65 0.4555482475732582 1104 66-67 0.5592873446540274 1104 68-69 0.9883397736631885 1104 70-71 1.2492837750894488 1104 72-73 0.5242193401400286 1104 74-75 1.1312246985082446 1104 76-77 1.470777706870404 1104 78-79 0.6442961516477688 1104 80-81 0.975138940849714 1104 82-83 0.7452124372261117 1104 84-85 0.9588305831628183 1104 86-87 1.3502149790347069 1104 88-89 1.2858243768234239 1104 90-91 1.1846616196231743 1104 92-93 -0.21935445939837095 1104 94-95 0.26345870435664764 1104 96-97 0.3178196279376664 1104 98-99 0.05240445028693941 1104 100-101 0.29440491558348647 1105 1 -0.11173206524791368 1105 2 -0.015915332784054215 1105 3 -0.10470399233489402 1105 4 0.08596812096180173 1105 5 -0.37113866184556343 1105 6 -0.27030745120805477 1105 7 0.03987128909025017 1105 8 0.18776412100464768 1105 9 0.27733141823042473 1105 10-11 -0.1727131328453737 1105 12-13 -0.4545708973203588 1105 14-15 0.3333772861311459 1105 16-17 0.09947897930940286 1105 18-19 -0.13635471895270257 1105 20-21 0.00773211289064335 1105 22-23 -0.030851370263938804 1105 24-25 -0.2941318539511997 1105 26-27 0.3714765262884683 1105 28-29 0.20284491930254944 1105 30-31 0.6331487863132494 1105 32-33 0.706254247962903 1105 34-35 0.7591388301084905 1105 36-37 0.5257076905659019 1105 38-39 0.9473146239926535 1105 40-41 0.8923243768191611 1105 42-43 1.3753351255801292 1105 44-45 1.1746779735049913 1105 46-47 1.1722048017914801 1105 48-49 2.147913574238796 1105 50-51 1.7792364334405235 1105 52-53 2.4434123194945947 1105 54-55 2.922453757615962 1105 56-57 3.3206534509930385 1105 58-59 2.251849203781422 1105 60-61 2.626405618906915 1105 62-63 2.291878718500527 1105 64-65 1.670287957016356 1105 66-67 2.14478976596153 1105 68-69 2.5382035751159755 1105 70-71 2.867852177026492 1105 72-73 3.1038954902610953 1105 74-75 3.159145400687425 1105 76-77 3.686501072487836 1105 78-79 3.945597604432269 1105 80-81 3.0828114590095197 1105 82-83 3.496196093400446 1105 84-85 2.766033972993327 1105 86-87 2.602106625523813 1105 88-89 2.3878976213754797 1105 90-91 2.6079364380251135 1105 92-93 3.181371932853448 1105 94-95 3.4998388496350934 1105 96-97 3.360343841012728 1105 98-99 3.7035848376961376 1105 100-101 2.852213632290505 1106 1 0.4814882737351347 1106 2 0.11120331128374161 1106 3 0.5478383805464624 1106 4 0.1368155785889229 1106 5 0.01869184662901091 1106 6 0.17037051489363364 1106 7 -0.05334904989280176 1106 8 0.2979336125300662 1106 9 0.3875009097558504 1106 10-11 0.47559195190039105 1106 12-13 0.3971240179338835 1106 14-15 0.3757501674870767 1106 16-17 0.22236033524161058 1106 18-19 0.7619503657930622 1106 20-21 0.7662066891618338 1106 22-23 0.8759282907530164 1106 24-25 0.7312918748623574 1106 26-27 1.04520533984779 1106 28-29 1.2791161057432276 1106 30-31 0.23060641343189303 1106 32-33 0.3799830615222177 1106 34-35 0.2082913724813693 1106 36-37 -0.7539533263832467 1106 38-39 -0.2984480878717548 1106 40-41 -0.8068281655537106 1106 42-43 -0.4466987727249503 1106 44-45 -0.12193219598653116 1106 46-47 -0.2896596049881808 1106 48-49 -0.5046287986425604 1106 50-51 -0.6148313631696496 1106 52-53 -0.1243842906748931 1106 54-55 -0.17924115763827686 1106 56-57 -0.10307536256628325 1106 58-59 0.8662559834424393 1106 60-61 0.6942022290764065 1106 62-63 0.2622177015513749 1106 64-65 0.2677455841349996 1106 66-67 1.2930948507072948 1106 68-69 0.08481374460749791 1106 70-71 0.9483606516027656 1106 72-73 1.396268371617026 1106 74-75 0.5066030278060687 1106 76-77 0.6398909029963136 1106 78-79 0.23797048578819968 1106 80-81 -0.018883456244715546 1106 82-83 0.3351791442479026 1106 84-85 0.5414577018068876 1106 86-87 1.1021066255238132 1106 88-89 0.2904399942568361 1106 90-91 0.5443771159912139 1106 92-93 0.973744814209379 1106 94-95 1.0676354598045883 1106 96-97 1.4323777393178112 1106 98-99 1.3815509393910546 1106 100-101 1.3395017678837267 1107 1 0.2795692374555472 1107 2 0.1544166464847514 1107 3 0.2417030674774061 1107 4 -0.06160156666951622 1107 5 0.08109554462593138 1107 6 0.39218029217337147 1107 7 -0.07211918576617649 1107 8 0.06141589296149874 1107 9 0.44500196032315387 1107 10-11 0.38286187766030366 1107 12-13 0.39113578428771234 1107 14-15 0.6121278114148012 1107 16-17 0.35858389596439366 1107 18-19 0.620824157640655 1107 20-21 0.20222714020539456 1107 22-23 0.019926049542753788 1107 24-25 0.028427328007062158 1107 26-27 0.46354124123454454 1107 28-29 0.5253690823505934 1107 30-31 0.1697785663944984 1107 32-33 0.42795896851199444 1107 34-35 0.6684748715708793 1107 36-37 0.8958925904118189 1107 38-39 1.0947792548933464 1107 40-41 0.4645683885855192 1107 42-43 0.8345030762735064 1107 44-45 0.32266788805885227 1107 46-47 0.9614259812283805 1107 48-49 0.4579359863413295 1107 50-51 0.424494872997883 1107 52-53 0.037403074502300626 1107 54-55 0.3445717013055578 1107 56-57 0.01217187093981309 1107 58-59 -0.13055729572831254 1107 60-61 0.31428207219169124 1107 62-63 0.4609850359119285 1107 64-65 1.3006633597338215 1107 66-67 1.2856008039220335 1107 68-69 1.7203719460362734 1107 70-71 1.211983007675041 1107 72-73 1.3783737084989411 1107 74-75 1.2257863868199372 1107 76-77 1.0487366797157378 1107 78-79 0.9362825743160066 1107 80-81 1.4859491533644729 1107 82-83 1.4987174549356723 1107 84-85 1.9102768641545538 1107 86-87 1.4500895362956285 1107 88-89 1.8235678833169295 1107 90-91 1.8472206515003897 1107 92-93 1.3709643127385824 1107 94-95 0.9956015614995017 1107 96-97 1.0887021545020126 1107 98-99 1.2983460087284975 1107 100-101 0.9760755177086331 1108 1 -0.19857952287503267 1108 2 -0.004525502275580351 1108 3 -0.1276192465721806 1108 4 0.115324053165196 1108 5 0.08764099917138424 1108 6 -0.1739684681572129 1108 7 -0.32507786345212253 1108 8 -0.21494774340213496 1108 9 -0.07195671736279508 1108 10-11 0.044812290883442074 1108 12-13 -0.06440140579493203 1108 14-15 -0.030351527428173597 1108 16-17 -0.12462271560584526 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-1.8667440986072101 2316 62-63 -1.55833314590625 2316 64-65 -1.487904133379125 2316 66-67 -1.8174983696316893 2316 68-69 -2.043505464432048 2316 70-71 -1.5063709868153161 2316 72-73 -1.5963163741456867 2316 74-75 -1.4321681586346102 2316 76-77 -1.2991627693200734 2316 78-79 -1.7676086102569961 2316 80-81 -1.8323015353407612 2316 82-83 -1.7437756580119874 2316 84-85 -1.3495027501705152 2316 86-87 -1.6750826400129135 2316 88-89 -1.1692351469861002 2316 90-91 -2.340933618472061 2316 92-93 -2.038402078445987 2316 94-95 -1.7293984385004997 2316 96-97 -1.416704181586141 2316 98-99 -1.9910479306654416 2316 100-101 -0.8520236558450858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 7.0 5 24.0 6 92.0 7 164.0 8 236.0 9 420.0 10 728.0 11 1145.0 12 1485.0 13 1967.0 14 2317.0 15 3070.0 16 3822.0 17 4424.0 18 4108.0 19 3009.0 20 2773.0 21 2755.0 22 2817.0 23 2714.0 24 2703.0 25 2677.0 26 2431.0 27 2313.0 28 2638.0 29 2958.0 30 3364.0 31 3863.0 32 4474.0 33 5422.0 34 7163.0 35 10136.0 36 10044.0 37 12527.0 38 7362.0 39 365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.188012048701054 24.882086416523933 16.602401302744706 15.327500232030308 2 14.768630695585367 27.394530887232264 38.42646922252146 19.410369194660902 3 18.795933004261066 34.442053748470656 33.48858794245454 13.273425304813738 4 10.510551050886356 25.20692884685156 35.015482749601325 29.267037352660758 5 8.009729236694087 44.893839839197334 32.663040724287626 14.433390199820956 6 20.616103747078526 42.51554603059425 16.940743678228806 19.927606544098413 7 17.94733333895831 43.617479053991346 20.308979994768773 18.126207612281576 8 24.556400239624026 34.32614180004894 20.154574372041616 20.962883588285422 9 23.953965187016426 20.736759506914503 21.655599524126934 33.65367578194214 10-11 21.458584699499657 32.77069499405159 24.37457285329778 21.39614745315097 12-13 25.7026299580658 32.39227465638421 26.080206549160895 15.824888836389103 14-15 21.895223550654325 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38.90426447508311 14.980930522976188 40-41 12.830183903099645 33.26076776063486 39.20903525758669 14.7000130786788 42-43 13.775544643007814 32.64018967582983 38.38530856072495 15.1989571204374 44-45 13.342586420742666 32.470321214320066 38.17615740936052 16.01093495557674 46-47 15.04020452416912 32.01132307899999 36.84894405116479 16.099528345666094 48-49 14.481222420033918 31.672558830229754 36.48950801137371 17.356710738362626 50-51 12.557480235236545 31.901636024603654 37.76525282866039 17.77563091149942 52-53 13.589382293134433 32.65721108007999 37.233270614838126 16.52013601194745 54-55 13.478851492165814 31.690277508247622 35.84193251714915 18.988938482437415 56-57 14.89212700073406 32.26866578354525 34.13292383499692 18.706283380723765 58-59 14.263535804385793 32.67366413823944 35.248356803550486 17.81444325382428 60-61 14.468144348163586 34.53707844311882 33.427551700571215 17.56722550814637 62-63 13.05740007931218 35.07496688294704 33.332630211189766 18.53500282655102 64-65 13.010150271264523 35.23570060496629 33.12802166741198 18.626127456357207 66-67 13.736194196711077 34.0308304997511 32.11383828753196 20.119137016005872 68-69 13.826053206658848 33.29972409487086 32.472008707464624 20.40221399100566 70-71 14.824627274951697 32.6053206658848 32.260650191108596 20.30940186805491 72-73 14.531847214370691 31.141420362979776 33.25331803339549 21.073414389254044 74-75 13.992693154684058 32.040854209029774 31.926104675199756 22.040347961086407 76-77 13.965271391084974 30.891671377585027 33.29677098186789 21.84628624946211 78-79 14.176208034154861 31.008530277845747 32.89092888060142 21.92433280739797 80-81 14.667814171687002 30.255319867024415 32.424610607671404 22.652255353617175 82-83 14.251843601815759 30.49789905330836 32.321672657317876 22.928584687558008 84-85 14.403661983715141 30.475045352908914 31.634814158545332 23.486478504830615 86-87 14.418245560744705 30.587302189990424 31.517974290499634 23.476477958765233 88-89 14.570237683409411 30.79632801491744 30.258439575089223 24.374994726583925 90-91 15.046260162258626 30.03050259672867 30.787793898636878 24.13544334237583 92-93 15.19720549956336 30.485536013365 30.308770360745367 24.00848812632627 94-95 14.97017355866992 29.959753288502267 30.39934525265991 24.670727900167904 96-97 14.592596967574819 30.17870552400881 30.25126772922485 24.977429779191525 98-99 14.882486700388547 30.38977037340162 29.305973329058332 25.4217695971515 100-101 14.544081539668746 30.21372100675841 30.110783924940304 25.13141352863254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2927.0 1 1744.0 2 608.5 3 646.0 4 505.0 5 464.0 6 769.0 7 1278.5 8 1685.0 9 1694.0 10 1301.5 11 883.0 12 744.5 13 721.5 14 671.5 15 665.0 16 690.5 17 702.5 18 737.5 19 754.5 20 819.5 21 920.5 22 1032.0 23 1171.0 24 1231.5 25 1356.5 26 1691.5 27 2057.0 28 2258.5 29 2418.5 30 2679.0 31 2950.5 32 3230.0 33 3610.5 34 3971.0 35 4056.5 36 4016.0 37 4076.5 38 3792.5 39 3277.0 40 3092.5 41 3044.5 42 3019.0 43 2985.5 44 2971.0 45 3062.0 46 3077.0 47 3643.5 48 4851.0 49 4773.5 50 3849.5 51 3061.5 52 1928.0 53 1123.5 54 755.0 55 583.5 56 522.0 57 444.0 58 416.0 59 398.5 60 357.0 61 321.5 62 253.0 63 155.5 64 93.5 65 64.0 66 54.5 67 45.0 68 26.0 69 27.0 70 29.5 71 22.5 72 18.0 73 14.5 74 12.5 75 8.0 76 6.5 77 10.0 78 10.0 79 6.0 80 5.5 81 9.0 82 7.0 83 4.0 84 4.0 85 3.5 86 2.0 87 1.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.06074975320412761 3 0.0033749862891182003 4 0.0 5 0.09534336266758915 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.2187328613977503E-4 26-27 0.009281212295075052 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012656198584193253 36-37 0.0 38-39 0.004218732861397751 40-41 0.004640606147537526 42-43 8.437465722795501E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.437465722795501E-4 82-83 8.437465722795501E-4 84-85 0.0033749862891182003 86-87 0.0021093664306988755 88-89 0.0 90-91 0.0037968595752579755 92-93 4.2187328613977503E-4 94-95 0.0 96-97 0.0 98-99 4.2187328613977503E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 118519.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.09451649102676 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8456604529966 33.61064470675588 2 9.424734415714573 7.93459276571689 3 3.6279815594307476 4.581543887477957 4 1.7959510924032873 3.0239877150499077 5 1.1805973140910002 2.484833655363275 6 0.795750651433153 2.0098043351698887 7 0.5772699939867709 1.7009930897155732 8 0.4028863499699339 1.3567444882255166 9 0.3307276007215875 1.252963659835132 >10 1.8179995991180598 14.034036736725758 >50 0.10623371417117658 3.1336747694462495 >100 0.06814992984566046 6.054725402678052 >500 0.012026458208057728 3.7318910891924504 >1k 0.014030867909400682 15.089563698647474 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4255 3.5901416650494857 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3561 3.004581543887478 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2981 2.515208531965339 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2311 1.9498983285380405 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2018 1.7026805828601321 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1547 1.305275947316464 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1211 1.0217770990305353 RNA PCR Primer, Index 17 (95% over 21bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 953 0.8040904833824112 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 861 0.7264657987326927 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 804 0.6783722441127583 RNA PCR Primer, Index 17 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 645 0.5442165391203099 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 627 0.5290291008192779 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 533 0.4497169230250002 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 454 0.38306094381491573 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 435 0.36702975894160433 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.3501548274960133 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 308 0.2598739442621014 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 290 0.24468650596106953 RNA PCR Primer, Index 17 (96% over 26bp) GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 289 0.24384275938878996 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 288 0.24299901281651043 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 272 0.2294990676600376 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 269 0.226967827943199 No Hit TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 261 0.22021785536496258 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 253 0.21346788278672618 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.2016554307748125 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 228 0.19237421847973743 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 225 0.18984297876289877 No Hit CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 225 0.18984297876289877 TruSeq Adapter, Index 12 (95% over 23bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 219 0.18478049932922147 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177 0.14934314329348036 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 171 0.14428066385980307 No Hit TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT 168 0.1417494241429644 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 162 0.1366869447092871 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 160 0.13499945156472803 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 159 0.13415570499244847 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 159 0.13415570499244847 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 147 0.12403074612509386 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.11728077354685745 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.11306204068545972 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 132 0.11137454754090062 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 130 0.10968705439634151 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 121 0.10209333524582556 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.437465722795501E-4 0.0 16-17 0.0 0.0 0.0 0.0012656198584193252 0.0 18-19 0.0 0.0 0.0 0.002109366430698875 0.0 20-21 0.0 0.0 0.0 0.008015592436655726 0.0 22-23 0.0 0.0 0.0 0.031218623174343353 0.0 24-25 0.0 0.0 0.0 0.07424969836060041 0.0 26-27 0.0 0.0 0.0 0.13078071870333027 0.0 28-29 0.0 0.0 0.0 0.23245218066301604 0.0 30-31 0.0 0.0 0.0 0.44718568330816155 0.0 32-33 0.0 0.0 0.0 0.7454500966089825 0.0 34-35 0.0 0.0 0.0 1.1669015094626176 0.0 36-37 0.0 0.0 0.0 1.6980399767125947 0.0 38-39 0.0 0.0 0.0 2.271365772576549 0.0 40-41 0.0 0.0 0.0 2.837941595862267 0.0 42-43 0.0 0.0 0.0 3.342923919371578 0.0 44-45 0.0 0.0 0.0 3.8348281710105554 0.0 46-47 0.0 0.0 0.0 4.402669614154693 0.0 48-49 0.0 0.0 0.0 4.918198769817497 0.0 50-51 0.0 0.0 0.0 5.425712333043647 0.0 52-53 0.0 0.0 0.0 5.896101047089497 0.0 54-55 0.0 0.0 0.0 6.392224031589872 0.0 56-57 0.0 0.0 0.0 6.969346687029084 0.0 58-59 0.0 0.0 0.0 7.480657109830491 0.0 60-61 0.0 0.0 0.0 8.015170563369587 0.0 62-63 0.0 0.0 0.0 8.519309140306618 0.0 64-65 0.0 0.0 0.0 9.025978956960486 0.0 66-67 0.0 0.0 0.0 9.598882879538301 0.0 68-69 0.0 0.0 0.0 10.219880356736052 0.0 70-71 0.0 0.0 0.0 10.87715893654182 0.0 72-73 0.0 0.0 0.0 11.475797129574161 0.0 74-75 0.0 0.0 0.0 12.123372623798716 0.0 76-77 0.0 0.0 0.0 12.73635450855981 0.0 78-79 0.0 0.0 0.0 13.443836009416211 0.0 80-81 0.0 0.0 0.0 14.15722373627857 0.0 82-83 0.0 0.0 0.0 14.838127220108168 0.0 84-85 0.0 0.0 0.0 15.522405690226883 0.0 86-87 0.0 0.0 0.0 16.18727798918317 0.0 88-89 0.0 0.0 0.0 16.847087808705776 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGGGA 60 1.4813431E-6 47.497887 8 ATGGGAC 60 0.0039213947 31.692003 5 GCCTGAG 30 0.0039455397 31.665258 14-15 ATGGGGG 125 2.1272535E-7 30.39865 7 GAGTACC 35 0.008380185 27.164577 1 TCAAAAT 35 0.008380185 27.141653 12-13 TCCGGCC 35 0.008380185 27.141653 74-75 CATGGGA 660 0.0 24.489277 4 AGTACTT 2095 0.0 22.445303 12-13 ACATGGG 1315 0.0 21.690348 3 GGTATCA 2790 0.0 21.639164 1 GTTATAG 110 0.0028490596 21.608185 1 GTATCAA 3580 0.0 21.511612 1 GTACATG 1345 0.0 21.206545 1 TACATGG 1345 0.0 21.206545 2 CATGGGG 410 0.0 20.852732 6 CTCCCAC 70 4.8375718E-4 20.35624 92-93 ATGGGAT 165 3.9185143E-6 20.167639 5 AGTACAT 1045 0.0 20.016005 2 GAGTACT 2230 0.0 19.915033 12-13 >>END_MODULE