SRR938397_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR938397_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	118386
Sequences flagged as poor quality	0
Sequence length	101
%GC	34

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4283	3.6178264321794806	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3496	2.9530518811345936	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3024	2.554356089402463	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2372	2.0036152923487576	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	2072	1.7502069501461321	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1512	1.2771780447012315	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	1253	1.058402175932965	RNA PCR Primer, Index 17 (95% over 21bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	966	0.8159748618924535	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	825	0.6968729410572196	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT	818	0.6909600797391583	RNA PCR Primer, Index 17 (95% over 23bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	646	0.5456726302096532	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	630	0.5321575186255132	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	521	0.44008582095855925	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	421	0.35561637355768416	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	403	0.34041187302552667	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	391	0.33027553933742165	No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT	327	0.27621509300086156	RNA PCR Primer, Index 17 (96% over 26bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	310	0.2618552869427128	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	273	0.23060159140438902	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	268	0.22637811903434527	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	265	0.22384403561231903	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	261	0.22046525771628403	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	252	0.21286300745020528	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	251	0.2120183129761965	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	242	0.20441606271011775	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	237	0.200192590340074	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	230	0.19427972902201274	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG	207	0.17485175611981146	TruSeq Adapter, Index 12 (95% over 23bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	181	0.15288969979558392	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	180	0.1520450053215752	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	175	0.14782153295153144	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	157	0.13261703241937392	No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT	154	0.13008294899734765	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	154	0.13008294899734765	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	146	0.12332539320527765	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146	0.12332539320527765	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	136	0.11487844846519014	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	136	0.11487844846519014	No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	130	0.10981028162113764	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	127	0.10727619819911138	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	126	0.10643150372510264	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	125	0.10558680925109387	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGATGT	30	1.347702E-6	79.285164	1
TATAAGA	35	3.4020632E-6	67.843864	9
TACTGGG	30	1.2868614E-4	63.42813	2
AGATGTG	40	7.437571E-6	59.463875	2
ACTGGGG	25	0.004997307	57.03704	3
GGGGGCT	25	0.005014065	56.988846	7
CTATACA	35	2.7572495E-4	54.366966	1
GATGTGT	50	2.8007402E-5	47.53087	3
ATGTGTA	50	2.8007402E-5	47.53087	4
TGTGTAT	50	2.8007402E-5	47.53087	5
GTGTATA	50	2.8147822E-5	47.490704	6
GTACTGG	45	9.519664E-4	42.28542	1
GCTAGTA	45	9.519664E-4	42.28542	2
GGCTAGT	45	9.519664E-4	42.28542	1
GTATAAG	60	8.2552375E-5	39.57559	8
TGTATAA	65	1.3225195E-4	36.53131	7
CATGAGG	55	0.002562141	34.5679	4
ATGGGGC	60	0.0039242585	31.687244	3
ATGGGAC	75	3.0502523E-4	31.687244	5
TAAGAGA	40	4.4881253E-4	29.681692	10-11

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content