##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938396_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117897 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.013155551031833 31.0 31.0 34.0 26.0 34.0 2 30.062826026107533 31.0 31.0 34.0 26.0 34.0 3 29.977802658252543 31.0 31.0 34.0 25.0 34.0 4 33.59937911906155 37.0 35.0 37.0 28.0 37.0 5 33.62420587461937 37.0 35.0 37.0 28.0 37.0 6 33.693970160394244 37.0 35.0 37.0 29.0 37.0 7 33.63566502964452 37.0 35.0 37.0 28.0 37.0 8 33.62965978778086 37.0 35.0 37.0 28.0 37.0 9 34.92153320271084 39.0 35.0 39.0 28.0 39.0 10-11 34.801139978116495 38.0 35.0 39.0 27.0 39.0 12-13 34.64402402096745 38.0 35.0 39.0 27.0 39.0 14-15 35.41195704725311 39.0 35.5 41.0 26.5 41.0 16-17 35.33279472760121 39.0 35.5 40.5 27.0 41.0 18-19 35.393525704640496 39.0 35.0 41.0 27.0 41.0 20-21 35.43189394132166 39.0 35.0 41.0 27.0 41.0 22-23 35.216752758764 39.0 35.0 41.0 26.0 41.0 24-25 34.97819282933408 39.0 35.0 41.0 25.0 41.0 26-27 34.60781444820479 38.0 35.0 40.5 23.5 41.0 28-29 34.258496823498476 38.0 34.0 40.0 22.0 41.0 30-31 33.66374038355514 37.0 34.0 40.0 20.0 41.0 32-33 32.96487612068161 35.5 33.0 40.0 17.0 41.0 34-35 32.42895069425006 35.0 33.0 40.0 10.5 41.0 36-37 31.708627021891992 35.0 32.0 39.0 2.0 41.0 38-39 31.19520852947912 35.0 31.0 39.0 2.0 41.0 40-41 30.691357710543947 35.0 31.0 39.0 2.0 41.0 42-43 29.941262288268575 35.0 29.5 39.0 2.0 41.0 44-45 29.030921058211828 35.0 25.0 39.0 2.0 40.5 46-47 28.03489062486747 35.0 21.5 38.5 2.0 40.0 48-49 27.143773802556467 35.0 16.5 38.0 2.0 40.0 50-51 26.18283756160038 34.0 8.0 37.5 2.0 39.5 52-53 26.086965741282647 33.5 2.0 38.0 2.0 40.0 54-55 26.010072351289686 34.0 2.0 38.0 2.0 40.0 56-57 25.53179470215527 34.0 2.0 38.0 2.0 40.0 58-59 25.089166815101315 33.5 2.0 38.0 2.0 40.0 60-61 24.499703130698833 33.0 2.0 38.0 2.0 40.0 62-63 23.854610380247166 33.0 2.0 37.0 2.0 40.0 64-65 23.276236884738374 32.0 2.0 36.5 2.0 39.5 66-67 22.622148994461266 31.0 2.0 36.0 2.0 39.0 68-69 21.86054352528054 30.5 2.0 35.0 2.0 39.0 70-71 21.1715480461759 29.0 2.0 35.0 2.0 38.0 72-73 20.422182074183397 28.5 2.0 35.0 2.0 37.0 74-75 19.674673655818214 26.0 2.0 35.0 2.0 36.5 76-77 18.904471699873618 25.5 2.0 34.0 2.0 36.0 78-79 18.071074751690034 22.5 2.0 34.0 2.0 35.5 80-81 17.2821318608616 19.0 2.0 33.0 2.0 35.0 82-83 16.472683783302372 9.0 2.0 33.0 2.0 35.0 84-85 15.701646352324486 2.0 2.0 32.0 2.0 35.0 86-87 14.959417966530108 2.0 2.0 31.5 2.0 35.0 88-89 14.130669143404837 2.0 2.0 31.0 2.0 34.0 90-91 13.255070103564975 2.0 2.0 30.5 2.0 34.0 92-93 12.374589684215884 2.0 2.0 29.0 2.0 34.0 94-95 11.357939557410281 2.0 2.0 28.0 2.0 34.0 96-97 10.214386286334683 2.0 2.0 24.5 2.0 33.0 98-99 9.012587258369594 2.0 2.0 10.5 2.0 33.0 100-101 6.48876137645572 2.0 2.0 2.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5995139960070794 1101 2 0.16042807670770287 1101 3 0.5300624136977881 1101 4 0.45462648648604187 1101 5 0.3440987329342775 1101 6 0.35921656231661814 1101 7 0.5567259294427771 1101 8 0.24888841354011504 1101 9 0.651060778879966 1101 10-11 0.5860034415802815 1101 12-13 0.4529294171222418 1101 14-15 0.8487685407272991 1101 16-17 0.6171431726026242 1101 18-19 0.4813816423223898 1101 20-21 0.8214981322806949 1101 22-23 1.536100070186933 1101 24-25 1.331484064026938 1101 26-27 1.5550462651086008 1101 28-29 1.352712104591582 1101 30-31 1.3857322659553901 1101 32-33 0.9785959947266392 1101 34-35 1.2889636570208793 1101 36-37 1.403592659262923 1101 38-39 1.5019645745169434 1101 40-41 1.4726199931252175 1101 42-43 1.7095934144855427 1101 44-45 0.7387110038054914 1101 46-47 0.5020206216152481 1101 48-49 -0.25308133122808485 1101 50-51 -0.16382174451747034 1101 52-53 -0.21905530382144534 1101 54-55 0.8173960554271602 1101 56-57 0.326127869798448 1101 58-59 -0.1907843780382379 1101 60-61 -0.29035375409763375 1101 62-63 -0.09077808352415673 1101 64-65 0.008258415307214051 1101 66-67 0.141614430494311 1101 68-69 -0.07426723579995098 1101 70-71 0.6485165006054139 1101 72-73 1.0907198279048451 1101 74-75 0.947783168205298 1101 76-77 1.005236543806447 1101 78-79 0.8756967303202536 1101 80-81 0.7039960272061592 1101 82-83 0.4709509373685741 1101 84-85 1.0449732389125046 1101 86-87 1.8422658842436146 1101 88-89 1.6913830040783626 1101 90-91 2.4799899567228767 1101 92-93 2.574465920971619 1101 94-95 2.0745265336621586 1101 96-97 2.38345099226456 1101 98-99 2.7053239050962574 1101 100-101 2.584262028057169 1102 1 0.03722672575520747 1102 2 -0.7009572194121034 1102 3 -0.15654412136007423 1102 4 -0.6953905318938141 1102 5 -0.2842198511365197 1102 6 -0.654227957765066 1102 7 -0.35392757634347305 1102 8 6.753434243904621E-4 1102 9 -0.04745862207305862 1102 10-11 -0.4756030934775808 1102 12-13 -0.4289204807674807 1102 14-15 -0.14940198343879985 1102 16-17 -0.5483180254913194 1102 18-19 -0.4055312235727797 1102 20-21 -0.43369247357362894 1102 22-23 -1.009381549962832 1102 24-25 -0.8144059972392341 1102 26-27 -0.5420435919370092 1102 28-29 -1.8725261527263157 1102 30-31 -1.065765351471434 1102 32-33 -1.0987525416926935 1102 34-35 -1.584164321195594 1102 36-37 -0.7983304176601571 1102 38-39 -0.7212740231685544 1102 40-41 -0.8158415453363155 1102 42-43 -0.861671051137332 1102 44-45 -0.9187090098092092 1102 46-47 -0.8511533062268235 1102 48-49 -0.6367181590020756 1102 50-51 -0.7865242632376876 1102 52-53 -1.6234545550127315 1102 54-55 -1.2501669125782868 1102 56-57 -1.1213704283635657 1102 58-59 -1.2837472779701642 1102 60-61 -0.9143751972562413 1102 62-63 -0.8406078997256543 1102 64-65 -0.5233957712142256 1102 66-67 -0.38850810184061046 1102 68-69 -0.22151876745413546 1102 70-71 0.004541007072401015 1102 72-73 0.1936384800491595 1102 74-75 0.24930631320189534 1102 76-77 0.33743531848309516 1102 78-79 1.1967484661949968 1102 80-81 1.146473903312355 1102 82-83 1.6615993376408689 1102 84-85 1.7853578542971178 1102 86-87 1.6861647950673078 1102 88-89 2.7557550258618857 1102 90-91 2.815209493822943 1102 92-93 2.726184777336492 1102 94-95 1.7054404206601177 1102 96-97 1.5525285960766766 1102 98-99 0.7028562400179723 1102 100-101 0.1408481410592115 1103 1 1.1080963227712246 1103 2 1.5950275681253778 1103 3 1.160942896851001 1103 4 1.191832462328378 1103 5 1.9226111296284927 1103 6 1.5164002876821598 1103 7 1.179898211705968 1103 8 1.4620956608382727 1103 9 2.160835095472464 1103 10-11 1.7220492139896848 1103 12-13 2.0006487432506574 1103 14-15 2.4452005178410943 1103 16-17 2.1755904071862204 1103 18-19 2.609242417910437 1103 20-21 2.2767587807231564 1103 22-23 1.7263019137978617 1103 24-25 1.6733070138044326 1103 26-27 1.8957169580520201 1103 28-29 2.3543808903512797 1103 30-31 1.9561391318168049 1103 32-33 2.119012396506676 1103 34-35 2.4741988763470104 1103 36-37 2.9082811525877723 1103 38-39 3.0172617773141504 1103 40-41 3.341183248052115 1103 42-43 3.120154444364754 1103 44-45 3.19977266941261 1103 46-47 3.0704805707570166 1103 48-49 3.8768852930567235 1103 50-51 3.731759946518764 1103 52-53 3.5749291208447964 1103 54-55 3.9961945296801815 1103 56-57 3.556022974693551 1103 58-59 3.1140074210717437 1103 60-61 2.78931089943066 1103 62-63 3.303691083672284 1103 64-65 3.3394726556123615 1103 66-67 3.322637952426927 1103 68-69 2.46717427723247 1103 70-71 2.0474357631610403 1103 72-73 1.8985313345799888 1103 74-75 1.3221871732911197 1103 76-77 0.397480663323293 1103 78-79 1.284549241445493 1103 80-81 0.7659794982805366 1103 82-83 0.7939404478580876 1103 84-85 0.8018867799169556 1103 86-87 1.0944511989289314 1103 88-89 1.5331264878672997 1103 90-91 1.525404769183142 1103 92-93 1.4513731046969838 1103 94-95 2.4574333995235698 1103 96-97 2.492080998621841 1103 98-99 1.7840746997561432 1103 100-101 1.3805906993858406 1104 1 0.9755823720754542 1104 2 0.7042314955111237 1104 3 0.6956607042960812 1104 4 0.630908537768093 1104 5 0.7885431773787204 1104 6 0.5664815195815791 1104 7 0.1913413140581639 1104 8 -0.07269278304107729 1104 9 0.38503513785433086 1104 10-11 0.18429403987087767 1104 12-13 1.0731217248145484 1104 14-15 1.234986489445248 1104 16-17 0.8901132580727094 1104 18-19 0.8627918987326453 1104 20-21 0.7071818929644564 1104 22-23 0.6103522069390621 1104 24-25 0.6877874828303518 1104 26-27 0.34884968391201454 1104 28-29 0.7127548396343215 1104 30-31 0.6784673086904363 1104 32-33 0.18692932805997486 1104 34-35 0.34345083650806174 1104 36-37 0.3597892404595022 1104 38-39 1.2760030360554104 1104 40-41 1.406380676885906 1104 42-43 1.8404694828616108 1104 44-45 1.4630699781644694 1104 46-47 1.9950761771708017 1104 48-49 1.9985212328744808 1104 50-51 1.7314774007816744 1104 52-53 1.7889575166913758 1104 54-55 1.66568665371776 1104 56-57 2.0152304339010136 1104 58-59 2.629194254440396 1104 60-61 3.0242829980391193 1104 62-63 2.6613586686125927 1104 64-65 2.461249868298669 1104 66-67 3.1352041740840555 1104 68-69 3.081181482148768 1104 70-71 2.5502259023148177 1104 72-73 2.801724101409121 1104 74-75 2.148103681025809 1104 76-77 1.005236543806447 1104 78-79 1.5220642516877732 1104 80-81 2.165534488744619 1104 82-83 2.6253312792489183 1104 84-85 2.6186911876304535 1104 86-87 2.4977146021923335 1104 88-89 2.3799513801467373 1104 90-91 2.3170626062955275 1104 92-93 2.4067308782365764 1104 94-95 2.547282943918571 1104 96-97 2.8781091119226794 1104 98-99 3.0632298880022404 1104 100-101 2.311826130621273 1105 1 0.7824487083535558 1105 2 0.7075353811679506 1105 3 1.0372537517714804 1105 4 1.012722801935297 1105 5 0.7558634388166325 1105 6 0.9149696327755734 1105 7 1.01745637546734 1105 8 0.9008761317043081 1105 9 0.9517233516013661 1105 10-11 0.9114720905783358 1105 12-13 0.6119064694816458 1105 14-15 0.6863978232095249 1105 16-17 0.9533180271598312 1105 18-19 1.2023738853734542 1105 20-21 1.410622243463628 1105 22-23 0.524343444459717 1105 24-25 0.7686608578213736 1105 26-27 0.7697359871512575 1105 28-29 1.141900857015635 1105 30-31 1.2776197901958568 1105 32-33 1.436013577144223 1105 34-35 1.334293327350558 1105 36-37 1.176459498306226 1105 38-39 1.337735421317202 1105 40-41 1.4446626563443594 1105 42-43 0.8503093162308559 1105 44-45 1.2346709262360065 1105 46-47 1.2035800915570682 1105 48-49 0.7728155659923424 1105 50-51 1.5383437370597761 1105 52-53 1.9952708112399655 1105 54-55 2.1992641872952916 1105 56-57 2.542272019766127 1105 58-59 2.781137082853814 1105 60-61 3.062385418494479 1105 62-63 3.2127125434958828 1105 64-65 3.327909352605211 1105 66-67 3.854445716855011 1105 68-69 2.8845643737669526 1105 70-71 3.269395621484538 1105 72-73 3.4244706359203576 1105 74-75 2.9824066329758203 1105 76-77 2.767679982720473 1105 78-79 3.180722586816696 1105 80-81 3.498113674264985 1105 82-83 3.3371031674913922 1105 84-85 3.9534250811878735 1105 86-87 3.796895813138251 1105 88-89 3.5271538508786175 1105 90-91 3.878866815158558 1105 92-93 4.618502766479054 1105 94-95 3.1234518730286744 1105 96-97 2.635632634152085 1105 98-99 2.770045947759476 1105 100-101 2.058971142472166 1106 1 1.1959937874021236 1106 2 1.4855584548041847 1106 3 1.096718215523083 1106 4 1.6285182726659642 1106 5 1.31020042784953 1106 6 1.3099988308954948 1106 7 1.4384078068873691 1106 8 1.0665220510890094 1106 9 1.2894245337691501 1106 10-11 1.80308297221913 1106 12-13 1.6816614901339761 1106 14-15 1.8439608484196057 1106 16-17 1.68893261197681 1106 18-19 2.1992760360642407 1106 20-21 1.6000183408856472 1106 22-23 1.5055427038244815 1106 24-25 1.5251770236097286 1106 26-27 1.755991506308078 1106 28-29 1.374061517890155 1106 30-31 1.5229552125003707 1106 32-33 1.829476046877872 1106 34-35 1.9256976857040584 1106 36-37 1.9855678874245903 1106 38-39 1.635520637098434 1106 40-41 1.836700827545041 1106 42-43 0.9217022801961043 1106 44-45 2.131800964692065 1106 46-47 2.8502116255265904 1106 48-49 2.2851357483025545 1106 50-51 2.7146006805151224 1106 52-53 2.8145985423324014 1106 54-55 2.7355837998860935 1106 56-57 2.984374284400797 1106 58-59 3.0384366128352944 1106 60-61 2.5371397269975446 1106 62-63 2.6787786309478108 1106 64-65 2.565624777758323 1106 66-67 2.9173641045490726 1106 68-69 2.5265313300409886 1106 70-71 2.578112328506325 1106 72-73 2.478024260760126 1106 74-75 3.119637796627721 1106 76-77 2.3451974303774996 1106 78-79 2.7885063782993917 1106 80-81 2.9370468746637854 1106 82-83 3.5520298161169457 1106 84-85 3.6709510746361005 1106 86-87 2.4727254670337064 1106 88-89 2.8376509310666282 1106 90-91 2.6978028641544274 1106 92-93 2.7825819574774844 1106 94-95 2.486113886986802 1106 96-97 2.094826481182423 1106 98-99 1.6272600198289826 1106 100-101 1.923733996766427 1107 1 -0.4492039527108709 1107 2 -0.24918730790767896 1107 3 -0.5712196375842602 1107 4 -0.5261427442831916 1107 5 -0.6559012670657225 1107 6 -0.4401424120423556 1107 7 -0.45481253209567996 1107 8 -0.3286756890239815 1107 9 -0.11368281086362231 1107 10-11 -0.26944527636843674 1107 12-13 -0.36918596749314503 1107 14-15 -0.5602058182470628 1107 16-17 -0.7617029812435305 1107 18-19 -0.636246562805816 1107 20-21 -0.4172839190013633 1107 22-23 -0.3280024939156334 1107 24-25 -0.5169133718705012 1107 26-27 -0.5292486066862736 1107 28-29 -0.29055712617763874 1107 30-31 -0.25946004173691506 1107 32-33 -0.5175578514272061 1107 34-35 -0.5847542916970667 1107 36-37 -0.43166375099348997 1107 38-39 -0.3050867075343362 1107 40-41 0.013325121330350242 1107 42-43 0.20093956833169457 1107 44-45 0.5816597217542139 1107 46-47 0.05939241648703941 1107 48-49 -0.2517992799460309 1107 50-51 -0.0808089240046499 1107 52-53 -0.2812347910009301 1107 54-55 0.20104990158100833 1107 56-57 0.8783714595420413 1107 58-59 0.728125878372019 1107 60-61 1.0372103484664699 1107 62-63 0.5044142241681513 1107 64-65 0.6858225178713155 1107 66-67 0.688409302289184 1107 68-69 0.46996353343081765 1107 70-71 0.453324192913108 1107 72-73 -0.09485709517207752 1107 74-75 -0.03587067794855159 1107 76-77 -0.18771217414227337 1107 78-79 0.1997351918587107 1107 80-81 0.21649602720615846 1107 82-83 0.3715919630096032 1107 84-85 0.21869118763045137 1107 86-87 -0.12183667985894964 1107 88-89 0.2384983886937455 1107 90-91 -0.2514202996873802 1107 92-93 -0.5553417713360744 1107 94-95 0.30016755930318517 1107 96-97 0.9465920179055871 1107 98-99 0.4104521102244618 1107 100-101 -0.3622123309171892 1108 1 0.5857553968826252 1108 2 0.4211378953443514 1108 3 0.5163819884319985 1108 4 0.43015806872493556 1108 5 0.27092800122696303 1108 6 0.4153453928356967 1108 7 0.1825858418880557 1108 8 0.7611617093499987 1108 9 1.0135529614941063 1108 10-11 0.6891929350136721 1108 12-13 0.37674899185645216 1108 14-15 -0.21498224101129182 1108 16-17 0.354049051276796 1108 18-19 0.3768631932917472 1108 20-21 0.7244843736815696 1108 22-23 1.165595067059975 1108 24-25 1.0810541078042917 1108 26-27 1.558251393313732 1108 28-29 1.2428778331719528 1108 30-31 1.0597472753362567 1108 32-33 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0.8026050450386855 2314 60-61 0.8070020151331363 2314 62-63 1.1661329741681499 2314 64-65 0.8985829345379841 2314 66-67 0.9261697189558511 2314 68-69 0.49965103343081907 2314 70-71 1.5595741929131073 2314 72-73 1.8785804048279218 2314 74-75 1.7070980720514513 2314 76-77 1.1714024091910602 2314 78-79 1.2903601918587135 2314 80-81 0.8094647772061592 2314 82-83 0.06560237967626747 2314 84-85 -0.3201108957028822 2314 86-87 0.029465403474384644 2314 88-89 0.5056858886937459 2314 90-91 0.5100380336459533 2314 92-93 0.5501269786639256 2314 94-95 0.48558422596985196 2314 96-97 0.44685243457225354 2314 98-99 0.044566693557795745 2314 100-101 0.14195433574947725 2315 1 -2.5425881766294474 2315 2 -1.9694163155412667 2315 3 -1.9108506808412606 2315 4 -2.139692362603803 2315 5 -1.8467409617222117 2315 6 -2.2480940659863755 2315 7 -2.318934669500262 2315 8 -1.5232049511105004 2315 9 -3.7825123274030616 2315 10-11 -2.516169194943508 2315 12-13 -3.5390332957374184 2315 14-15 -3.2953521286796317 2315 16-17 -2.7003480194114715 2315 18-19 -2.8519272243834237 2315 20-21 -2.8426974050064473 2315 22-23 -2.2190012216510056 2315 24-25 -2.194903193753454 2315 26-27 -1.5931480977804213 2315 28-29 -2.218705981139472 2315 30-31 -2.374250118072794 2315 32-33 -2.519275408679114 2315 34-35 -2.6956957675240396 2315 36-37 -2.4586357611716103 2315 38-39 -2.7284009568982057 2315 40-41 -2.361643072053873 2315 42-43 -2.459556614874412 2315 44-45 -2.5121697947852297 2315 46-47 -2.5340554206630834 2315 48-49 -3.039744572566896 2315 50-51 -2.828677880747655 2315 52-53 -3.0365464958355375 2315 54-55 -2.8001905564342593 2315 56-57 -2.946405894147528 2315 58-59 -3.225245622900246 2315 60-61 -3.214348175706558 2315 62-63 -3.3670552414807062 2315 64-65 -3.367548983400944 2315 66-67 -3.619542351654836 2315 68-69 -3.337002115424145 2315 70-71 -3.991236875789184 2315 72-73 -4.295620453950704 2315 74-75 -3.974229456574502 2315 76-77 -4.0167834209616124 2315 78-79 -4.163719006614569 2315 80-81 -3.902874201801474 2315 82-83 -4.925004729102358 2315 84-85 -4.558153596084562 2315 86-87 -3.5006216671363024 2315 88-89 -3.9359290922222847 2315 90-91 -4.102915210628856 2315 92-93 -3.681773069045999 2315 94-95 -4.071429132808774 2315 96-97 -3.030157345962097 2315 98-99 -2.8659473808696845 2315 100-101 -1.8990761986016684 2316 1 -0.4912804554431105 2316 2 -1.3778758324978426 2316 3 -0.6107005119012001 2316 4 -0.4954050393651599 2316 5 -0.9181963490329323 2316 6 -0.4361806634084786 2316 7 -0.4335010566858486 2316 8 -0.6863259622480271 2316 9 -0.2986554884592465 2316 10-11 -0.6223824348383786 2316 12-13 -1.0495138363456036 2316 14-15 -1.2871866925639992 2316 16-17 -0.6975636369812364 2316 18-19 -0.37060448630308684 2316 20-21 -1.184018891678953 2316 22-23 -1.2284806359921348 2316 24-25 -1.5412303117612112 2316 26-27 -1.5059562569595002 2316 28-29 -1.29404073273502 2316 30-31 -1.499828894195936 2316 32-33 -1.0253447366731088 2316 34-35 -1.008798007544062 2316 36-37 -1.4745599258568767 2316 38-39 -1.641083975293899 2316 40-41 -1.2666612174674619 2316 42-43 -1.012585021832237 2316 44-45 -2.0610998410873194 2316 46-47 -1.3243507529118652 2316 48-49 -1.9391626679241725 2316 50-51 -1.3747296890319731 2316 52-53 -1.193598178979073 2316 54-55 -1.7954664918616174 2316 56-57 -1.1544837317147945 2316 58-59 -0.6785681106990182 2316 60-61 -0.8889508537193223 2316 62-63 -1.5412824971433245 2316 64-65 -0.5544780285767708 2316 66-67 -1.0125469818638209 2316 68-69 -0.9495036796839358 2316 70-71 -1.5526184300377146 2316 72-73 -1.4809226689425685 2316 74-75 -1.3461165795878927 2316 76-77 -1.4285591686777934 2316 78-79 -0.3820271032232547 2316 80-81 -1.3123974154167932 2316 82-83 -1.5611266162253727 2316 84-85 -0.5589044407848505 2316 86-87 -0.6846099039026647 2316 88-89 -0.37379669327346754 2316 90-91 -0.460504999140932 2316 92-93 0.11781396636884267 2316 94-95 -0.13378052812850783 2316 96-97 0.06551278293290963 2316 98-99 0.4179657714266476 2316 100-101 0.4636756472248864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4153.0 3 729.0 4 582.0 5 450.0 6 500.0 7 594.0 8 657.0 9 761.0 10 946.0 11 1296.0 12 1815.0 13 2302.0 14 2667.0 15 3274.0 16 3820.0 17 4375.0 18 3586.0 19 2766.0 20 2771.0 21 2879.0 22 2824.0 23 2842.0 24 3062.0 25 3153.0 26 3345.0 27 3599.0 28 4006.0 29 4231.0 30 4650.0 31 5086.0 32 5680.0 33 6308.0 34 7644.0 35 7558.0 36 6566.0 37 5070.0 38 1334.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.71486231032545 23.002775932832645 16.324358384849877 13.958003371992032 2 15.535702165207358 26.87419398628928 38.0150003393742 19.575103509129164 3 20.46430329051199 33.38537512515485 31.640843755833487 14.50947782849967 4 11.711497666525245 22.96902842596521 37.02503182011031 28.294442087399236 5 9.109426852142622 43.14607504477054 32.552218157745095 15.192279945341742 6 22.498323870628273 41.303222411759215 17.48266585194049 18.71578786567202 7 19.416017514383178 42.92127013220644 22.130577193965006 15.532135159445378 8 25.046035827329582 33.30363279957231 20.17005677044882 21.48027460264929 9 26.19940602460755 20.696648281714044 22.7874416631311 30.316504030547303 10-11 22.172488990335253 32.139311503508665 24.733349738228778 20.954849767927296 12-13 26.439756148262532 32.588655039729844 26.51951280561012 14.452076006397501 14-15 22.829895726394202 31.873870510677648 30.639386406251322 14.656847356676819 16-17 15.062316000237555 35.509090754833835 31.11388259648587 18.314710648442738 18-19 14.145416442180018 32.182689003618655 38.75990039156128 14.911994162640047 20-21 16.891805893782934 30.124392251363986 38.72323996843525 14.260561886417825 22-23 16.04357934393402 30.909006058343376 38.99485804469938 14.052556553023232 24-25 15.664419335814026 32.15535858999436 38.73060457301421 13.449617501177407 26-27 14.519064731413636 31.817545407875365 40.331866761138244 13.331523099572756 28-29 13.897774421544753 33.46301025819425 39.33029009732133 13.308925222939664 30-31 14.345474215650919 32.703591353598775 39.04796249549224 13.902971935258055 32-33 13.850330901069066 33.44815883251315 39.41583234345834 13.285677922959444 34-35 14.35213860865286 32.922100511636984 38.892047141875324 13.833713737834833 36-37 14.045828083171056 32.4411239377702 39.21181806225526 14.30122991680349 38-39 13.989454886340983 33.158995372196706 38.81161744058299 14.039932300879327 40-41 13.585771609389448 33.20028504288368 38.915988700085684 14.297954647641184 42-43 13.565468944191592 32.94083214211484 38.02496680848176 15.46873210521181 44-45 14.157621719748734 32.127481793465584 37.90309924628977 15.811797240495915 46-47 15.001845167146694 32.804950986422114 37.23632137297403 14.956882473457163 48-49 14.877372176081849 32.27516818378479 36.67339684586476 16.1740627942686 50-51 14.098719455036711 32.23970240796746 36.96698747449726 16.69459066249857 52-53 14.340186027967816 33.00151418113339 35.82671320900365 16.831586581895145 54-55 14.454463710087884 31.575531878796102 35.97943741305012 17.990566998065898 56-57 15.195381920439074 31.323456434051977 35.51951274340562 17.96164890210333 58-59 15.842667560151158 30.62333266887493 35.54527294396071 17.988726827013203 60-61 15.280764483579127 30.978466027900424 35.97068292000153 17.77008656851892 62-63 14.821286937638112 30.633798336507347 36.01957848572119 18.52533624013335 64-65 14.967596362649294 31.287323561346366 35.508279044516826 18.236801031487513 66-67 15.415445557991884 30.30880088895298 34.624209548610374 19.651544004444766 68-69 15.298972745086395 30.064807824442052 34.79051973500047 19.845699695471087 70-71 16.29374811773339 29.95847348708182 34.695210665399806 19.052567729784986 72-73 16.185459002973353 28.586577084420238 35.50544831418258 19.72251559842383 74-75 15.751611808618934 29.993213437393962 34.48040380047506 19.774770953512046 76-77 16.288765608034744 28.795975841476658 34.42547163409338 20.48978691639522 78-79 16.69105515190845 28.95994774501945 33.93053311108001 20.418463991992095 80-81 17.324307473056024 28.400497100951362 33.36429610590116 20.910899320091445 82-83 17.291517328842946 28.531194462235383 33.32117423005306 20.85611397886861 84-85 17.61305317804338 28.347485303723058 32.65372772231035 21.385733795923215 86-87 18.073321344959794 28.425168798561394 32.214229973195806 21.287279883283006 88-89 18.85241381650668 28.20141931816736 31.119802160791014 21.826364704534946 90-91 19.65951561790746 27.68673400559131 30.961764443859952 21.691985932641277 92-93 20.69884035595219 27.44717129987021 30.884111943400548 20.969876400777054 94-95 20.625615094851867 27.09217090304408 30.444649947398783 21.837564054705265 96-97 20.318398927641233 27.14917155195086 30.1537273799323 22.378702140475603 98-99 21.15083623530809 27.067895605257913 29.15265164850547 22.628616510928534 100-101 22.491825555225276 25.76584992993333 29.656885642702452 22.085438872138944 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2601.0 1 1693.0 2 743.0 3 674.0 4 511.5 5 456.5 6 712.5 7 1135.5 8 1546.5 9 1653.0 10 1341.5 11 957.5 12 800.0 13 754.0 14 734.0 15 736.0 16 715.5 17 697.5 18 757.5 19 820.0 20 845.5 21 926.5 22 1045.0 23 1107.5 24 1213.0 25 1423.5 26 1665.0 27 1976.0 28 2226.0 29 2400.0 30 2660.5 31 2877.5 32 3077.0 33 3425.5 34 3856.0 35 4067.0 36 4078.0 37 4113.5 38 3778.5 39 3288.5 40 3061.5 41 2897.5 42 2733.0 43 2553.5 44 2438.5 45 2398.5 46 2567.5 47 3118.5 48 3973.0 49 4130.5 50 3390.5 51 2658.0 52 2078.0 53 1630.0 54 1315.0 55 1067.5 56 887.5 57 805.5 58 740.5 59 668.0 60 601.0 61 545.0 62 496.0 63 409.0 64 319.5 65 273.5 66 241.0 67 201.5 68 158.0 69 118.5 70 106.0 71 101.5 72 79.5 73 65.0 74 57.0 75 48.5 76 36.0 77 28.0 78 26.5 79 18.5 80 13.0 81 11.5 82 10.0 83 8.5 84 5.0 85 4.0 86 5.0 87 4.0 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38932288353393213 2 0.027990534110282704 3 0.0364725141437017 4 0.039865306157069305 5 0.0627666522473006 6 0.05598106822056541 7 0.04325809817043691 8 0.04580269218046261 9 0.039865306157069305 10-11 0.03901710815372741 12-13 0.03265562312866315 14-15 0.028838732113624605 16-17 0.025445940100257004 18-19 0.030111029118637455 20-21 0.037320712147043605 22-23 0.0364725141437017 24-25 0.04537859317879166 26-27 0.041137603162082155 28-29 0.033927920133676 30-31 0.03774481114871455 32-33 0.0313833261236503 34-35 0.033927920133676 36-37 0.03689661314537266 38-39 0.019084455075192755 40-41 0.016963960066838 42-43 0.017388059068508953 44-45 0.011450673045115652 46-47 0.01823625707185085 48-49 0.016963960066838 50-51 0.014843465058483253 52-53 0.009754277038431852 54-55 0.011026574043444702 56-57 0.010602475041773751 58-59 0.005513287021722351 60-61 0.011450673045115652 62-63 0.010602475041773751 64-65 0.007633782030077101 66-67 0.004665089018380451 68-69 0.008481980033419 70-71 0.017388059068508953 72-73 0.013995267055141352 74-75 0.014419366056812303 76-77 0.007633782030077101 78-79 0.011450673045115652 80-81 0.011450673045115652 82-83 0.013147069051799453 84-85 0.008481980033419 86-87 0.0042409900167095 88-89 0.019932653078534653 90-91 0.030111029118637455 92-93 0.013571168053470403 94-95 0.024597742096915107 96-97 0.022053148086889404 98-99 0.014843465058483253 100-101 0.12892609650796882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 117897.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.90879326870065 #Duplication Level Percentage of deduplicated Percentage of total 1 83.48305643231244 40.83055548487239 2 7.71045055669245 7.542176645716175 3 2.906593597169713 4.264739560803074 4 1.6423294370642711 3.212974036659118 5 0.972911102632583 2.37919539937403 6 0.6590128680933717 1.9338914476195321 7 0.4162186535326558 1.4249726456143923 8 0.33124067843640526 1.2960465491064233 9 0.22198328188408312 0.9771240998498689 >10 1.5087926190558774 13.142828061782744 >50 0.08150948631681176 2.784634044971458 >100 0.04855884291214318 4.9907970516637405 >500 0.008671221948596996 3.102708296224671 >1k 0.008671221948596996 12.117356675742386 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3991 3.3851582313375235 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3640 3.0874407321645165 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2881 2.4436584476280143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2116 1.7947869750714607 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1658 1.4063122895408704 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 896 0.7599854109943426 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 829 0.7031561447704352 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 662 0.5615070782123379 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.5555696921889446 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 616 0.5224899700586104 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 465 0.39441207155398356 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 464 0.3935638735506417 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 369 0.31298506323316116 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.24937021298251863 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 282 0.23919183694241583 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 280 0.23749544093573205 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 269 0.22816526289897113 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 268 0.22731706489562925 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 260 0.22053148086889404 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 255 0.21629049085218452 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 245 0.20780851081876553 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 240 0.20356752080205603 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 182 0.15437203660822582 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 175 0.14843465058483252 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 168 0.14249726456143924 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 151 0.12807789850462692 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 149 0.1263815024979431 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 147 0.12468510649125931 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 145 0.12298871048457552 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 137 0.11620312645784033 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 137 0.11620312645784033 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.11365853244781463 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 121 0.10263195840436992 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 120 0.10178376040102802 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.481980033419001E-4 0.0 16-17 0.0 0.0 0.0 8.481980033419001E-4 0.0 18-19 0.0 0.0 0.0 8.481980033419001E-4 0.0 20-21 0.0 0.0 0.0 0.005937386023393301 4.2409900167095005E-4 22-23 0.0 0.0 0.0 0.033503821132005056 8.481980033419001E-4 24-25 0.0 0.0 0.0 0.0780342163074548 8.481980033419001E-4 26-27 0.0 0.0 0.0 0.12214051248123362 8.481980033419001E-4 28-29 0.0 0.0 0.0 0.24131233195077056 8.481980033419001E-4 30-31 0.0 0.0 0.0 0.47541498087313505 8.481980033419001E-4 32-33 0.0 0.0 0.0 0.7939133311280185 8.481980033419001E-4 34-35 0.0 0.0 0.0 1.2002001747287887 8.481980033419001E-4 36-37 0.0 0.0 0.0 1.7405023028575792 8.481980033419001E-4 38-39 0.0 0.0 0.0 2.335513202201922 8.481980033419001E-4 40-41 0.0 0.0 0.0 2.87030204330899 8.481980033419001E-4 42-43 0.0 0.0 0.0 3.332994054131997 8.481980033419001E-4 44-45 0.0 0.0 0.0 3.7973824609616873 8.481980033419001E-4 46-47 0.0 0.0 0.0 4.346590668125567 8.481980033419001E-4 48-49 0.0 0.0 0.0 4.817764658981993 8.481980033419001E-4 50-51 0.0 0.0 0.0 5.282577164813354 8.481980033419001E-4 52-53 0.0 0.0 0.0 5.702435176467595 8.481980033419001E-4 54-55 0.0 0.0 0.0 6.170216375310653 8.481980033419001E-4 56-57 0.0 0.0 0.0 6.743598225569777 8.481980033419001E-4 58-59 0.0 0.0 0.0 7.219437305444583 8.481980033419001E-4 60-61 0.0 0.0 0.0 7.706727058364504 8.481980033419001E-4 62-63 0.0 0.0 0.0 8.166874475177485 8.481980033419001E-4 64-65 0.0 0.0 0.0 8.666887198147535 8.481980033419001E-4 66-67 0.0 0.0 0.0 9.23136296937157 8.481980033419001E-4 68-69 0.0 0.0 0.0 9.848851115804473 8.481980033419001E-4 70-71 0.0 0.0 0.0 10.467611559242389 8.481980033419001E-4 72-73 0.0 0.0 0.0 11.052868181548302 8.481980033419001E-4 74-75 0.0 0.0 0.0 11.666963535967836 8.481980033419001E-4 76-77 0.0 0.0 0.0 12.281482989389044 8.481980033419001E-4 78-79 0.0 0.0 0.0 12.942229233992382 8.481980033419001E-4 80-81 0.0 0.0 0.0 13.625876824685955 8.481980033419001E-4 82-83 0.0 0.0 0.0 14.20434786296513 8.481980033419001E-4 84-85 0.0 0.0 0.0 14.81547452437297 8.481980033419001E-4 86-87 0.0 0.0 0.0 15.378677998591993 8.481980033419001E-4 88-89 0.0 0.0 0.0 15.974961194941347 8.481980033419001E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGGAC 25 0.0050111925 56.996944 4 CCGTTAA 20 5.380835E-4 47.497456 36-37 AAAGTGA 45 9.592425E-4 42.21996 8 CGTATTA 25 0.0016136351 37.997963 28-29 GCACCAA 25 0.0016136351 37.997963 52-53 CGATGGT 25 0.0016136351 37.997963 64-65 ACCCCGT 25 0.0016136351 37.997963 24-25 CCAGCTT 55 0.0025709565 34.543606 9 GGTCTAC 60 0.0038888694 31.745779 1 CCCGTAT 30 0.0039456734 31.664968 26-27 TTAACCG 30 0.0039456734 31.664968 32-33 GTCTACA 85 6.3748535E-4 27.939678 2 ATGGGGA 205 3.092282E-11 27.803387 5 CCCTCCA 35 0.008380471 27.141403 42-43 CGTTGAA 35 0.008380471 27.141403 22-23 ATATGGA 35 0.008380471 27.141403 56-57 TAGGGCA 35 0.008380471 27.141403 48-49 ATGGGAG 160 6.1458195E-8 26.71732 5 GGTATCA 2545 0.0 26.569162 1 CATGGGG 465 0.0 26.557718 4 >>END_MODULE