FastQCFastQC Report
Thu 26 May 2016
SRR938396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117897
Sequences flagged as poor quality0
Sequence length101
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41993.561583416032639No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34002.8838732113624603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29542.505576901871973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24022.037371604027244No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT20611.7481360848876561No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15631.32573347922339No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT12371.0492209301339306RNA PCR Primer, Index 17 (95% over 21bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9140.7752529750544968No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT7990.6777102046701783RNA PCR Primer, Index 17 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.6743174126568106No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.5759264442691502No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6410.543694920142158No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.4393665657311043No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4560.38677828952390647No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.36896613145372653No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.32316343927326396No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3230.2739679550794337No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2910.24682561897249292No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2890.24512922296580913No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2880.24428102496246723No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2690.22816526289897113No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT2640.22392427288226163RNA PCR Primer, Index 17 (96% over 26bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2620.22222787687557785No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC2570.21798688685886833No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2420.20526391680873984No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2400.20356752080205603No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG2380.20187112479537225TruSeq Adapter, Index 12 (95% over 23bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.19338914476195324No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2030.1721841946784057No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1790.1518274425982001No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.1518274425982001No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT1730.1467382545781487No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1670.1416490665580973No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT1640.13910447254807162No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC1580.13401528452802022No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.13316708652467832No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.12807789850462692No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG1290.10941754243110512No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1220.10348015640771183No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAAC250.00500180557.0239371
ATGGGGC459.590105E-442.2220345
CCTGGTC550.002570337434.54538
CTTTACA702.0371735E-433.9428181
ATGGGGT851.6199067E-533.5292635
ATGGGAG1351.1779775E-831.6665275
CGCTGTG300.003944724331.66652588-89
TACTGGG600.00393679131.6665252
TGGGAGA650.005823219729.230646
CTTGGAA350.00837846327.14273554-55
GTTACTG350.00837846327.14273522-23
CTTGGTA559.4456154E-525.90897616-17
TGGGGGA950.001220064424.9998878
GGTATCA27050.024.9457781
AGTACTC1751.4627585E-724.4284632
CATGGGG4600.023.7498954
ATGGGGG1401.7620274E-523.7498937
GGCCCCC500.001649804323.74989376-77
TTGGTAT500.001649804323.74989316-17
GTATCAA35700.023.1609841
Produced by FastQC (version 0.11.5)