##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938396_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117897 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62691162625003 31.0 31.0 34.0 30.0 34.0 2 31.841073140113828 31.0 31.0 34.0 30.0 34.0 3 31.80500776101173 31.0 31.0 34.0 30.0 34.0 4 35.54729127967633 37.0 35.0 37.0 33.0 37.0 5 35.48723037905969 37.0 35.0 37.0 33.0 37.0 6 35.5676395497765 37.0 35.0 37.0 33.0 37.0 7 35.49397355318626 37.0 35.0 37.0 33.0 37.0 8 35.501776974817 37.0 35.0 37.0 33.0 37.0 9 36.90526476500674 39.0 37.0 39.0 32.0 39.0 10-11 36.88975122352562 39.0 37.0 39.0 32.0 39.0 12-13 36.80455397507994 39.0 37.0 39.0 32.0 39.0 14-15 37.51330398568242 39.0 36.0 41.0 32.0 41.0 16-17 37.551248123361916 39.0 36.5 41.0 32.0 41.0 18-19 37.771287649388874 39.5 36.5 41.0 33.0 41.0 20-21 37.82547053784236 40.0 36.0 41.0 33.0 41.0 22-23 37.636445371807596 40.0 36.0 41.0 32.5 41.0 24-25 37.414136916121706 39.0 35.5 41.0 32.0 41.0 26-27 37.050060646157235 39.0 35.0 41.0 32.0 41.0 28-29 36.75077398067805 39.0 35.0 40.5 31.5 41.0 30-31 36.112097848121664 38.0 35.0 40.0 30.5 41.0 32-33 35.31109782267573 37.0 34.5 40.0 30.0 41.0 34-35 34.78066447831581 36.0 34.0 40.0 27.5 41.0 36-37 34.33545806933171 36.0 33.5 40.0 24.5 41.0 38-39 33.771609964630144 35.0 33.0 40.0 23.0 41.0 40-41 33.35914399857503 35.0 33.0 40.0 20.5 41.0 42-43 32.736643001942376 35.0 33.0 40.0 15.0 41.0 44-45 31.950978396396856 35.0 32.5 40.0 2.0 41.0 46-47 30.90030704767721 35.0 31.0 40.0 2.0 41.0 48-49 30.05033206951831 35.0 29.0 39.5 2.0 41.0 50-51 29.34450834202736 35.0 27.0 39.0 2.0 41.0 52-53 28.635037363122045 35.0 24.0 39.0 2.0 40.0 54-55 28.106258004868657 35.0 22.5 39.0 2.0 40.0 56-57 27.48330746329423 35.0 20.0 38.0 2.0 40.0 58-59 26.7061926936224 34.0 11.5 38.0 2.0 40.0 60-61 26.03453014071605 33.0 2.0 38.0 2.0 40.0 62-63 25.464528359500243 33.0 2.0 37.5 2.0 40.0 64-65 24.679181828205977 33.0 2.0 37.0 2.0 39.5 66-67 23.820678219123472 32.5 2.0 36.0 2.0 39.0 68-69 23.049963103386855 31.5 2.0 35.5 2.0 39.0 70-71 22.35760451919896 31.0 2.0 35.0 2.0 38.0 72-73 21.871527689423818 30.0 2.0 35.0 2.0 37.5 74-75 21.303985682417704 29.5 2.0 35.0 2.0 37.0 76-77 20.300779493965074 28.0 2.0 33.5 2.0 35.5 78-79 20.66188282992782 29.0 2.0 34.0 2.0 36.0 80-81 20.291381460088044 29.0 2.0 34.0 2.0 35.5 82-83 19.774790707142675 27.5 2.0 34.0 2.0 35.0 84-85 19.238398771809294 26.5 2.0 34.0 2.0 35.0 86-87 18.626008295376472 25.5 2.0 34.0 2.0 35.0 88-89 17.926758102411426 23.5 2.0 33.0 2.0 35.0 90-91 17.358965876994326 20.0 2.0 33.0 2.0 35.0 92-93 16.648158986233746 14.5 2.0 33.0 2.0 35.0 94-95 15.88660864992324 2.0 2.0 32.0 2.0 35.0 96-97 14.963396015165781 2.0 2.0 32.0 2.0 34.0 98-99 13.814096202617538 2.0 2.0 31.0 2.0 34.0 100-101 10.672582847739976 2.0 2.0 24.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3192145160922166 1101 2 0.4963397330187185 1101 3 0.30216974171847966 1101 4 0.04369341849576358 1101 5 0.514334710963972 1101 6 0.2549294756926912 1101 7 0.3861663114091698 1101 8 0.25882552297631634 1101 9 0.6171246241631678 1101 10-11 0.25961197082484944 1101 12-13 0.06237898924885599 1101 14-15 0.5617134602302158 1101 16-17 0.19363514664150472 1101 18-19 0.4890294852477979 1101 20-21 0.6823786455994778 1101 22-23 0.5711141148276653 1101 24-25 0.34047384802808267 1101 26-27 0.5075586422887923 1101 28-29 0.5373454913927915 1101 30-31 0.6216603066086606 1101 32-33 0.8796090615792878 1101 34-35 1.038850987810065 1101 36-37 1.1916153430492287 1101 38-39 1.3442251815229795 1101 40-41 0.9323012750795385 1101 42-43 1.5283129542968439 1101 44-45 1.210801328984921 1101 46-47 0.8818248800331894 1101 48-49 0.5679706650024379 1101 50-51 0.6571633197393965 1101 52-53 0.2920963673726149 1101 54-55 0.24320345870534155 1101 56-57 0.05240003008616867 1101 58-59 -0.31017937806520024 1101 60-61 0.5916096904957477 1101 62-63 0.11782674837454721 1101 64-65 0.4808868449739947 1101 66-67 -0.2549817429367067 1101 68-69 -0.37794922449182167 1101 70-71 -0.283831307857735 1101 72-73 -0.33642971024963586 1101 74-75 -0.7011321598309657 1101 76-77 -0.36369346616303844 1101 78-79 0.0893024049824902 1101 80-81 0.3882248461255102 1101 82-83 -0.025941849684457452 1101 84-85 -0.4654853184648253 1101 86-87 -0.1526074402951174 1101 88-89 -0.7254992825251421 1101 90-91 -0.45960076148745443 1101 92-93 0.10355866023771299 1101 94-95 0.21736088054825053 1101 96-97 1.1688236544561814 1101 98-99 1.1258392248039346 1101 100-101 1.2997687087727865 1102 1 0.04870736837267842 1102 2 0.1663533477226018 1102 3 0.06867757017321097 1102 4 0.005827523328981954 1102 5 -0.31599204192915664 1102 6 0.05709080993366911 1102 7 0.23453254694354797 1102 8 0.08021762644806785 1102 9 0.4511103287240914 1102 10-11 0.3797191866179048 1102 12-13 0.2308609497662104 1102 14-15 0.5586926978067979 1102 16-17 0.017707644939669365 1102 18-19 0.6213048426337693 1102 20-21 0.5794599934551528 1102 22-23 0.618340118912073 1102 24-25 0.6018336165781193 1102 26-27 0.4854772944331103 1102 28-29 0.9774407943199535 1102 30-31 0.5198053032049827 1102 32-33 0.6536475231177477 1102 34-35 0.024938462282499074 1102 36-37 0.13468886245018297 1102 38-39 0.19539944973264056 1102 40-41 -0.1352616929259014 1102 42-43 -0.5848592717072378 1102 44-45 0.3094228402170458 1102 46-47 0.009547820809228114 1102 48-49 0.37413131850822623 1102 50-51 0.06888047426628674 1102 52-53 0.2091743115523279 1102 54-55 0.9761425329054774 1102 56-57 0.7836798122509059 1102 58-59 0.8154162652295582 1102 60-61 -2.8956069553842667E-4 1102 62-63 0.13671715000830886 1102 64-65 -0.7939599896073553 1102 66-67 -0.25111006152077664 1102 68-69 0.03304464889142977 1102 70-71 -0.44444226769435957 1102 72-73 -0.3318347476900705 1102 74-75 -0.21785271803382855 1102 76-77 -0.2885147731746116 1102 78-79 -0.47757982782894715 1102 80-81 0.20876603060474963 1102 82-83 -0.0976317748035882 1102 84-85 -0.16817422248115932 1102 86-87 -0.3192173790289523 1102 88-89 0.19723727295477644 1102 90-91 0.9508825605002933 1102 92-93 1.6615487895773988 1102 94-95 1.9324732018552613 1102 96-97 1.8887436680708856 1102 98-99 1.5726993337215678 1102 100-101 0.17698109815331797 1103 1 0.10282862925178549 1103 2 0.07310387796468376 1103 3 0.19791036473183254 1103 4 0.2340144610895365 1103 5 0.5914167198641564 1103 6 0.602911039583347 1103 7 0.5814937112820218 1103 8 0.5351287651886523 1103 9 0.5262949992426371 1103 10-11 0.7290572982247241 1103 12-13 0.7562760013276844 1103 14-15 0.2887080565429869 1103 16-17 0.48293107798289014 1103 18-19 -0.03429378239365377 1103 20-21 0.4324183785937521 1103 22-23 0.5745311523356094 1103 24-25 0.6614767088036686 1103 26-27 0.9465684960713716 1103 28-29 0.7751077291550317 1103 30-31 0.677524896564158 1103 32-33 0.0462095065888235 1103 34-35 0.0074221893358128455 1103 36-37 -0.13745331556488338 1103 38-39 0.5637897079056842 1103 40-41 0.5987030551176886 1103 42-43 0.20011842155685855 1103 44-45 0.14142752097475153 1103 46-47 0.2439513898869734 1103 48-49 -0.7220801932302372 1103 50-51 -1.0403493948187688 1103 52-53 -1.3463731176877793 1103 54-55 -1.0745412965394152 1103 56-57 -0.6305306755718121 1103 58-59 -0.6423472102330336 1103 60-61 -0.6944678683090828 1103 62-63 -0.8171700729859097 1103 64-65 -0.5145041276897047 1103 66-67 0.7775595793773427 1103 68-69 -0.7039187095522159 1103 70-71 0.18950785298142492 1103 72-73 0.22126259744267074 1103 74-75 0.08617712179649573 1103 76-77 0.09812312633537346 1103 78-79 0.2881263083518455 1103 80-81 0.15292605400853887 1103 82-83 0.49452064236894344 1103 84-85 0.37669522826117685 1103 86-87 0.2674497752166438 1103 88-89 0.7061599673159265 1103 90-91 1.4091214254165472 1103 92-93 1.0277083741601523 1103 94-95 0.28093367139377 1103 96-97 -1.1636223086716022 1103 98-99 -0.9949888107968281 1103 100-101 -0.2446527788305186 1104 1 0.37583844771615205 1104 2 0.0882200748990627 1104 3 0.19960563915437746 1104 4 0.22638572618807018 1104 5 0.3455312921605511 1104 6 0.30086964663286153 1104 7 0.3712090464519022 1104 8 0.42656056571136247 1104 9 0.4728938549324013 1104 10-11 0.49839402210690054 1104 12-13 0.635028561898423 1104 14-15 0.5451536311703862 1104 16-17 0.5189556594620157 1104 18-19 0.3976833314016446 1104 20-21 0.44145984218067014 1104 22-23 0.35209702081057515 1104 24-25 0.2886576087118442 1104 26-27 0.5123663345964857 1104 28-29 -0.10368014963285077 1104 30-31 0.36418167413003033 1104 32-33 0.7177500872203098 1104 34-35 1.0949407313998094 1104 36-37 1.4384102148441045 1104 38-39 1.0440115063093032 1104 40-41 1.5840106767889424 1104 42-43 1.342415518399413 1104 44-45 1.8507585939421816 1104 46-47 1.3230641962725045 1104 48-49 1.339338186369961 1104 50-51 1.8906034906795668 1104 52-53 2.2381433759196234 1104 54-55 2.1422419202438014 1104 56-57 2.528895756581896 1104 58-59 2.2026411347553143 1104 60-61 2.8693874682735263 1104 62-63 2.4233823039301043 1104 64-65 3.3761859902731395 1104 66-67 2.6867917613367993 1104 68-69 2.1070935105509143 1104 70-71 2.473113136586708 1104 72-73 2.905023281203352 1104 74-75 2.9479063017074942 1104 76-77 2.853721063751493 1104 78-79 2.8093878750679586 1104 80-81 3.075190658091323 1104 82-83 2.5055752443326362 1104 84-85 3.087933484953979 1104 86-87 2.1444011067134277 1104 88-89 2.0779195208936585 1104 90-91 2.318177016290324 1104 92-93 2.4171270363060877 1104 94-95 2.653258316445685 1104 96-97 2.0155116886442155 1104 98-99 1.954364865829577 1104 100-101 2.335025119029197 1105 1 0.2412410190010732 1105 2 -0.04551547060118821 1105 3 -0.2130209499427984 1105 4 0.020180167041338848 1105 5 -0.021056368544755344 1105 6 -0.20103367879987388 1105 7 0.16299242647058065 1105 8 0.3599082637649431 1105 9 0.23953897451869466 1105 10-11 0.042134595259234686 1105 12-13 0.13994847858305093 1105 14-15 -0.11673676601413518 1105 16-17 -0.10440137565972663 1105 18-19 -0.4626915877968045 1105 20-21 -0.3572545153572193 1105 22-23 -0.20708562660736618 1105 24-25 -0.1184385630902085 1105 26-27 -0.03579139003182519 1105 28-29 -0.43421236251800366 1105 30-31 -0.4344967716072432 1105 32-33 0.03600046429421866 1105 34-35 0.04204264908995725 1105 36-37 0.01748799980423854 1105 38-39 -0.046773525652199055 1105 40-41 0.22593411283002496 1105 42-43 0.13771825487861378 1105 44-45 0.372485233315885 1105 46-47 0.2366244921857401 1105 48-49 1.2513901866572539 1105 50-51 1.0538585362875565 1105 52-53 1.8107211249679587 1105 54-55 1.368163057929646 1105 56-57 1.926834419226438 1105 58-59 2.2709453795301435 1105 60-61 2.2865030324479143 1105 62-63 1.769572061884567 1105 64-65 1.9590704260987515 1105 66-67 1.9514258200885024 1105 68-69 2.2514948608346153 1105 70-71 1.9339692739134335 1105 72-73 1.784287807526706 1105 74-75 0.8606406262065214 1105 76-77 1.0283960619559025 1105 78-79 1.1683263222417004 1105 80-81 1.0663922669399923 1105 82-83 1.447434039132606 1105 84-85 1.9238165561311682 1105 86-87 1.984270387759178 1105 88-89 1.1074031092007814 1105 90-91 1.1942453923586989 1105 92-93 0.7233469601730711 1105 94-95 0.38413528261676966 1105 96-97 0.3992292782441549 1105 98-99 0.9885169882169915 1105 100-101 1.0970376163358129 1106 1 0.05373211713524029 1106 2 0.4190907108544444 1106 3 0.5381540963469007 1106 4 0.4063074993301825 1106 5 0.6522095479913474 1106 6 0.26813025796126766 1106 7 0.422998123664712 1106 8 0.6302401253231267 1106 9 0.40183779235091066 1106 10-11 0.42722931111168094 1106 12-13 0.26674665548092946 1106 14-15 0.23894618513242705 1106 16-17 0.5097205442946944 1106 18-19 0.5613078424837781 1106 20-21 0.5789725178289373 1106 22-23 0.8053057706294879 1106 24-25 0.4986387763201279 1106 26-27 1.1554562954830558 1106 28-29 0.8398226752259106 1106 30-31 0.8640005934404726 1106 32-33 0.9417831163380868 1106 34-35 0.8330980543028943 1106 36-37 1.287606216582482 1106 38-39 1.7567088842609166 1106 40-41 1.9818449517418557 1106 42-43 1.7688605423020576 1106 44-45 1.101120755321297 1106 46-47 0.7307492607372339 1106 48-49 1.1276186441419433 1106 50-51 0.5491939585920704 1106 52-53 0.15397707141433514 1106 54-55 0.4616682566192907 1106 56-57 0.7698055059662181 1106 58-59 0.6989470886883851 1106 60-61 0.9292889603784076 1106 62-63 0.5767576479834133 1106 64-65 1.3374709388462236 1106 66-67 1.4621792740124455 1106 68-69 1.9659230049736287 1106 70-71 1.2457482227811134 1106 72-73 2.149228699137584 1106 74-75 1.7344121687218355 1106 76-77 1.5773919314901548 1106 78-79 2.034869549702176 1106 80-81 1.6830423167904414 1106 82-83 1.4783769247614345 1106 84-85 1.4161965589797667 1106 86-87 0.6462843458848013 1106 88-89 1.1203286183875036 1106 90-91 0.8425504771044636 1106 92-93 1.1753480996118988 1106 94-95 1.8565394985404264 1106 96-97 1.1475557274679158 1106 98-99 1.5203144529656036 1106 100-101 1.6705314206371948 1107 1 0.04229143916914069 1107 2 -0.08571154903255973 1107 3 0.0066569212056606375 1107 4 0.0154882902906337 1107 5 -0.3497678531385944 1107 6 0.24595511671832782 1107 7 0.30732015756301934 1107 8 0.1357485998993937 1107 9 0.1549451369836845 1107 10-11 0.334291458004337 1107 12-13 0.31109693796680205 1107 14-15 0.16684166535841172 1107 16-17 -0.020787930281578326 1107 18-19 -0.0696243609060474 1107 20-21 -0.0612110979902738 1107 22-23 -0.0484371672236108 1107 24-25 0.1831020531562899 1107 26-27 -0.1571849474547875 1107 28-29 0.10048651703382205 1107 30-31 -0.04885251390415846 1107 32-33 0.2744808564510848 1107 34-35 0.07667150063057449 1107 36-37 -0.47729491336102825 1107 38-39 0.09646877126656506 1107 40-41 0.27428845456672235 1107 42-43 -0.4620716610877693 1107 44-45 -0.2440063633227716 1107 46-47 -1.028431530223223 1107 48-49 -1.1060677965360206 1107 50-51 -1.4790546289785524 1107 52-53 -0.4851472223709763 1107 54-55 -0.10295038744850515 1107 56-57 -0.7229204827343452 1107 58-59 -0.4374229678087893 1107 60-61 -0.9205697966837398 1107 62-63 -0.7199937644459666 1107 64-65 -0.8255234114362615 1107 66-67 -0.7216484096033753 1107 68-69 -1.0135261475687436 1107 70-71 -0.8642800258064547 1107 72-73 -0.3329040692239964 1107 74-75 -0.8402347239335306 1107 76-77 -0.06978320975278152 1107 78-79 -0.12127451809443457 1107 80-81 0.07251971792038603 1107 82-83 0.49200686826425866 1107 84-85 0.4229762199967162 1107 86-87 -0.14171000439768378 1107 88-89 0.015526358500498105 1107 90-91 0.20257872569203172 1107 92-93 -0.537787493608441 1107 94-95 -0.5012288630414918 1107 96-97 -0.0782917301592061 1107 98-99 0.39058281454752475 1107 100-101 0.8831020421061204 1108 1 -0.09612319497720279 1108 2 -0.15359772789435056 1108 3 0.245884563482079 1108 4 -0.26642227881505676 1108 5 -0.35261338159387634 1108 6 -0.08270341986703755 1108 7 -0.33068797251828386 1108 8 -0.8238042456290557 1108 9 -0.07432315569924697 1108 10-11 -0.5900987858980997 1108 12-13 -0.228740460407181 1108 14-15 -0.6035851639098766 1108 16-17 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13 1951.0 14 2377.0 15 3167.0 16 3739.0 17 4532.0 18 4064.0 19 3204.0 20 2976.0 21 2937.0 22 2894.0 23 2902.0 24 3043.0 25 3022.0 26 2684.0 27 2655.0 28 2982.0 29 3419.0 30 3801.0 31 4326.0 32 5101.0 33 6127.0 34 8277.0 35 10122.0 36 9664.0 37 9896.0 38 3539.0 39 77.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35403305168143 24.631816485119955 16.637822796837355 15.376327666361261 2 14.752165319262648 27.536244178451152 38.4964498095537 19.2151406927325 3 18.851520420713346 34.446753467068156 33.44586284405615 13.255863268162349 4 10.596537655750359 25.366209487942864 34.946605935689625 29.090646920617147 5 8.033419568259891 44.626998600449554 33.059077993129485 14.280503838161076 6 20.5772835610745 42.64230642001069 16.639100231558054 20.14130978735676 7 17.78756032808299 43.21908106228317 20.541659244934138 18.451699364699696 8 24.327166933849036 34.30197545314979 20.448357464566527 20.92250014843465 9 24.083734106889914 20.685852905502262 21.418695980389664 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13.635476838774801 34.83938553409505 38.33710800654842 13.188029620581723 36-37 13.052070875425159 34.43090154965775 38.50437246070722 14.01265511420986 38-39 12.978695429039194 33.01084526935034 38.99376937791331 15.016689923697147 40-41 12.729192910129658 33.250202525310364 39.23816552361806 14.782439040941922 42-43 13.695353453101939 32.707347278063345 38.2351943271074 15.362104941727313 44-45 13.34215459256809 32.51863915112344 38.19350789248242 15.945698363826052 46-47 15.027948124210116 32.23873381002061 36.73206273272433 16.001255333044945 48-49 14.362960889590065 31.73066320601881 36.579387092122786 17.326988812268336 50-51 12.683528842973105 31.914298073742337 37.628183923255044 17.77398916002952 52-53 13.68525068491989 32.805330076253 36.830029602110315 16.679389636716795 54-55 13.594493498562304 31.632696336632826 35.633646318396565 19.139163846408305 56-57 15.05678685632374 32.225162641967145 33.9673613408314 18.750689160877716 58-59 14.2442131691222 32.869793124506984 35.21548470274901 17.670509003621806 60-61 14.579251380442251 34.65397762453667 33.371926342485395 17.39484465253569 62-63 13.027897232329916 35.162048228538474 33.37235044148706 18.437704097644552 64-65 13.170818595893024 35.429230599591165 32.83883389738501 18.5611169071308 66-67 13.760125535434073 33.86233512871623 32.177361211247295 20.2001781246024 68-69 13.748674668136902 33.06119852411044 32.518342592985285 20.671784214767378 70-71 14.539386074285183 32.65731952466984 32.347727253450046 20.455567147594937 72-73 14.495464203468325 31.009665339219904 33.228155442743784 21.266715014567986 74-75 14.153031883762946 32.0228674181701 31.796822650279484 22.027278047787476 76-77 13.94098238292747 30.986793557087967 33.25105812700917 21.821165932975394 78-79 14.102140003562432 31.165763335793105 32.824838630329864 21.907258030314598 80-81 14.66201286721828 30.55943136564699 32.15786728189556 22.62068848523917 82-83 14.146546276543859 30.562918541577428 32.29455148459003 22.995983697288676 84-85 14.26656770225851 30.463365496765988 31.578835754426894 23.691231046548616 86-87 14.26602313982289 30.89310555423608 31.305347266990125 23.535524038950904 88-89 14.595367142505747 30.946504151929226 29.833668371544654 24.624460334020373 90-91 14.907332796132152 29.907120743034056 30.730734976038 24.454811484795794 92-93 15.219216773963714 30.498655606164704 30.11781470266419 24.164312917207393 94-95 14.92701256181243 30.06522642645699 30.161496899836298 24.846264111894282 96-97 14.533024589260116 30.214933374046836 30.009669457238097 25.242372579454948 98-99 14.90453531472387 30.619099722639255 28.980805279184374 25.495559683452505 100-101 14.740409001077213 30.088975970550564 29.681416829944784 25.48919819842744 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2787.0 1 1730.0 2 661.5 3 604.0 4 482.5 5 450.5 6 713.5 7 1246.5 8 1642.5 9 1701.5 10 1324.0 11 890.5 12 786.5 13 765.5 14 748.0 15 700.5 16 672.0 17 681.0 18 691.5 19 726.0 20 814.5 21 904.5 22 1017.0 23 1175.0 24 1282.0 25 1417.5 26 1665.0 27 2017.5 28 2264.5 29 2431.5 30 2697.0 31 2927.5 32 3211.0 33 3611.0 34 3915.5 35 3979.5 36 3964.5 37 4035.0 38 3726.5 39 3241.0 40 3078.5 41 3005.0 42 2963.0 43 2913.0 44 2878.5 45 2933.0 46 3124.0 47 3719.5 48 4695.5 49 4734.5 50 3972.5 51 3117.0 52 1937.5 53 1086.5 54 765.0 55 623.5 56 527.0 57 474.5 58 442.5 59 412.5 60 371.5 61 313.0 62 244.5 63 169.0 64 99.5 65 62.5 66 54.5 67 52.0 68 35.0 69 26.5 70 25.0 71 16.5 72 15.5 73 14.5 74 9.5 75 6.5 76 5.5 77 7.0 78 8.5 79 5.0 80 7.5 81 10.5 82 8.5 83 7.0 84 4.5 85 2.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017812158070179905 2 0.013571168053470403 3 0.0016963960066838004 4 0.0 5 0.0016963960066838004 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016963960066838004 26-27 0.006785584026735202 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.005089188020051401 36-37 0.0 38-39 0.009754277038431852 40-41 0.008906079035089953 42-43 0.0025445940100257006 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0016963960066838004 68-69 0.0016963960066838004 70-71 0.0 72-73 0.0012722970050128503 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.240990016709501E-4 82-83 0.00212049500835475 84-85 0.008057881031748051 86-87 0.0042409900167095 88-89 0.0 90-91 0.0016963960066838004 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 117897.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.06213898572483 #Duplication Level Percentage of deduplicated Percentage of total 1 79.60677555958863 33.48431257792819 2 9.548296027424884 8.032435091647795 3 3.617664851784634 4.565001653986107 4 1.9883040935672516 3.345292925180454 5 1.17967332123412 2.480979159775058 6 0.7259528130671506 1.8321076872185043 7 0.5182496471062714 1.5259082080120783 8 0.38314176245210724 1.2892609650796882 9 0.34886065739060296 1.3206442912033385 >10 1.8814277071990322 14.11486297361256 >50 0.09881024400080661 2.8346777271686303 >100 0.07662835249042146 6.376752589124406 >500 0.012099213551119177 3.686268522523898 >1k 0.014115749142972372 15.111495627539293 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4199 3.561583416032639 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3400 2.8838732113624603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2954 2.505576901871973 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2402 2.037371604027244 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2061 1.7481360848876561 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1563 1.32573347922339 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1237 1.0492209301339306 RNA PCR Primer, Index 17 (95% over 21bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 914 0.7752529750544968 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 799 0.6777102046701783 RNA PCR Primer, Index 17 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 795 0.6743174126568106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 679 0.5759264442691502 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 641 0.543694920142158 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 518 0.4393665657311043 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 456 0.38677828952390647 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 435 0.36896613145372653 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.32316343927326396 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 323 0.2739679550794337 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 291 0.24682561897249292 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 289 0.24512922296580913 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 288 0.24428102496246723 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 269 0.22816526289897113 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 264 0.22392427288226163 RNA PCR Primer, Index 17 (96% over 26bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 262 0.22222787687557785 No Hit TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 257 0.21798688685886833 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 242 0.20526391680873984 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 240 0.20356752080205603 No Hit CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 238 0.20187112479537225 TruSeq Adapter, Index 12 (95% over 23bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 228 0.19338914476195324 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 203 0.1721841946784057 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 179 0.1518274425982001 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 179 0.1518274425982001 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 173 0.1467382545781487 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 167 0.1416490665580973 No Hit TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT 164 0.13910447254807162 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 158 0.13401528452802022 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 157 0.13316708652467832 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 151 0.12807789850462692 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 129 0.10941754243110512 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 122 0.10348015640771183 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.481980033419001E-4 0.0 16-17 0.0 0.0 0.0 0.0016963960066838002 0.0 18-19 0.0 0.0 0.0 0.0016963960066838002 0.0 20-21 0.0 0.0 0.0 0.006361485025064251 0.0 22-23 0.0 0.0 0.0 0.03435201913534695 0.0 24-25 0.0 0.0 0.0 0.07973061231413861 0.0 26-27 0.0 0.0 0.0 0.12256461148290457 0.0 28-29 0.0 0.0 0.0 0.24300872795745437 0.0 30-31 0.0 0.0 0.0 0.47880777288650267 0.0 32-33 0.0 0.0 0.0 0.7994266181497409 0.0 34-35 0.0 0.0 0.0 1.2074098577571948 0.0 36-37 0.0 0.0 0.0 1.7544975699127203 0.0 38-39 0.0 0.0 0.0 2.3554458552804567 0.0 40-41 0.0 0.0 0.0 2.896172082410918 0.0 42-43 0.0 0.0 0.0 3.3648014792573178 0.0 44-45 0.0 0.0 0.0 3.836823668117085 0.0 46-47 0.0 0.0 0.0 4.397906647327752 0.0 48-49 0.0 0.0 0.0 4.875866222210913 0.0 50-51 0.0 0.0 0.0 5.345343817060654 0.0 52-53 0.0 0.0 0.0 5.772411511743302 0.0 54-55 0.0 0.0 0.0 6.247402393614766 0.0 56-57 0.0 0.0 0.0 6.819087847867206 0.0 58-59 0.0 0.0 0.0 7.299167917758721 0.0 60-61 0.0 0.0 0.0 7.793667353707049 0.0 62-63 0.0 0.0 0.0 8.263144948556791 0.0 64-65 0.0 0.0 0.0 8.764429968531854 0.0 66-67 0.0 0.0 0.0 9.328057541752546 0.0 68-69 0.0 0.0 0.0 9.957420460232237 0.0 70-71 0.0 0.0 0.0 10.589327972721952 0.0 72-73 0.0 0.0 0.0 11.192820852099715 0.0 74-75 0.0 0.0 0.0 11.819639176569378 0.0 76-77 0.0 0.0 0.0 12.459180471089171 0.0 78-79 0.0 0.0 0.0 13.14537265579277 0.0 80-81 0.0 0.0 0.0 13.854890285588269 0.0 82-83 0.0 0.0 0.0 14.4719543330195 0.0 84-85 0.0 0.0 0.0 15.117008914561016 0.0 86-87 0.0 0.0 0.0 15.719653595935435 0.0 88-89 0.0 0.0 0.0 16.37446245451538 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATAAC 25 0.005001805 57.023937 1 ATGGGGC 45 9.590105E-4 42.222034 5 CCTGGTC 55 0.0025703374 34.5453 8 CTTTACA 70 2.0371735E-4 33.942818 1 ATGGGGT 85 1.6199067E-5 33.529263 5 ATGGGAG 135 1.1779775E-8 31.666527 5 CGCTGTG 30 0.0039447243 31.666525 88-89 TACTGGG 60 0.003936791 31.666525 2 TGGGAGA 65 0.0058232197 29.23064 6 CTTGGAA 35 0.008378463 27.142735 54-55 GTTACTG 35 0.008378463 27.142735 22-23 CTTGGTA 55 9.4456154E-5 25.908976 16-17 TGGGGGA 95 0.0012200644 24.999887 8 GGTATCA 2705 0.0 24.945778 1 AGTACTC 175 1.4627585E-7 24.428463 2 CATGGGG 460 0.0 23.749895 4 ATGGGGG 140 1.7620274E-5 23.749893 7 GGCCCCC 50 0.0016498043 23.749893 76-77 TTGGTAT 50 0.0016498043 23.749893 16-17 GTATCAA 3570 0.0 23.160984 1 >>END_MODULE