FastQCFastQC Report
Thu 26 May 2016
SRR938395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858165
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83020.4467848657142934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66950.36030169548990537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48060.25864226266235774No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26160.14078405308462918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113150.030.934681
GGTATCA76800.028.7948061
GTACATG100500.025.7900871
TATCAAC135850.025.4512352
TACATGG100100.025.3712162
ATCAACG136700.025.2929783
TCAACGC138550.024.9895784
CAACGCA140000.024.7647295
AACGCAG143700.024.1756786
ACATGGG104150.023.1973573
GAGTACT71500.022.49454112-13
ACGCAGA158300.021.8853247
CGCAGAG158650.021.7771328
GTACTTT77250.020.63011214-15
AGTACTT76000.020.56869712-13
GCAGAGT163650.020.4137259
CATGGGA58950.020.0885984
CATGGGG42600.019.8721334
AGAGTAC132700.019.73112910-11
TACCTGG13550.019.3044532
Produced by FastQC (version 0.11.5)