##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938395_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1858165 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.172591239206422 31.0 31.0 34.0 28.0 34.0 2 31.20094017484992 31.0 31.0 34.0 28.0 34.0 3 31.233402846356487 31.0 31.0 34.0 27.0 34.0 4 34.89420099937304 37.0 35.0 37.0 32.0 37.0 5 34.88408941078968 37.0 35.0 37.0 32.0 37.0 6 34.907044315224965 37.0 35.0 37.0 32.0 37.0 7 34.821639628343014 37.0 35.0 37.0 32.0 37.0 8 34.79911095085743 37.0 35.0 37.0 32.0 37.0 9 36.28519372606846 39.0 35.0 39.0 32.0 39.0 10-11 36.11866787933257 39.0 35.0 39.0 31.0 39.0 12-13 35.979559673118374 38.0 35.0 39.0 30.0 39.0 14-15 36.91799086733417 39.0 36.0 41.0 31.0 41.0 16-17 36.758665941937345 39.0 36.0 41.0 30.0 41.0 18-19 36.941123635414506 39.0 36.0 41.0 31.0 41.0 20-21 36.93134813108631 39.0 36.0 41.0 30.5 41.0 22-23 36.834704937397916 39.0 36.0 41.0 30.0 41.0 24-25 36.70591658975387 39.0 36.0 41.0 30.0 41.0 26-27 36.5018155007763 39.0 36.0 41.0 30.0 41.0 28-29 36.288695837022004 39.0 35.0 40.5 29.0 41.0 30-31 36.03193715305153 39.0 35.0 40.0 28.5 41.0 32-33 35.75825639811319 38.0 35.0 40.0 27.0 41.0 34-35 35.501877658873134 38.0 34.5 40.0 27.0 41.0 36-37 35.224291168975846 38.0 34.0 40.0 25.5 41.0 38-39 35.100672437593005 38.0 34.0 40.0 25.0 41.0 40-41 34.99105623020561 38.0 34.0 40.0 25.0 41.0 42-43 34.69418081817277 38.0 33.5 40.0 24.0 41.0 44-45 34.31662877085727 38.0 33.0 40.0 23.5 41.0 46-47 33.89923876512581 38.0 33.0 40.0 22.0 41.0 48-49 33.513703573148774 38.0 32.5 40.0 19.5 41.0 50-51 32.81311401301822 37.0 31.5 39.5 18.0 40.5 52-53 32.83514650205983 37.0 31.5 39.5 17.5 40.0 54-55 32.94904812005393 37.0 32.0 40.0 16.0 41.0 56-57 32.63692486942763 37.0 31.0 40.0 12.5 41.0 58-59 32.277588642558655 36.5 31.0 39.5 8.5 41.0 60-61 31.80698753878154 36.0 30.5 39.0 7.5 41.0 62-63 31.249845412005932 35.5 29.5 39.0 4.5 40.0 64-65 30.743301590547667 35.0 29.0 38.5 2.0 40.0 66-67 30.197439409309723 35.0 28.5 38.0 2.0 40.0 68-69 29.62289220817312 34.0 28.0 37.5 2.0 40.0 70-71 29.001318774166986 34.0 26.5 37.0 2.0 39.0 72-73 28.36527461231914 34.0 26.0 36.0 2.0 39.0 74-75 27.69320324083168 33.0 25.5 36.0 2.0 39.0 76-77 26.948457214510015 32.5 24.0 35.0 2.0 37.5 78-79 26.16730080482627 32.0 21.5 35.0 2.0 37.0 80-81 25.4638920655593 31.5 19.5 35.0 2.0 36.5 82-83 24.74298649474078 31.0 17.0 34.5 2.0 36.0 84-85 24.031711930856517 31.0 9.0 34.0 2.0 35.5 86-87 23.263664152537586 30.0 4.5 34.0 2.0 35.0 88-89 22.461668097289532 29.5 2.0 34.0 2.0 35.0 90-91 21.55227657393181 29.0 2.0 34.0 2.0 35.0 92-93 20.60726630842794 28.0 2.0 34.0 2.0 35.0 94-95 19.608494132652375 26.5 2.0 33.0 2.0 35.0 96-97 18.359221866734117 24.5 2.0 33.0 2.0 34.5 98-99 16.867634198254727 11.0 2.0 32.0 2.0 34.0 100-101 13.348916269545493 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3585168968478989 1101 2 0.22300582858417783 1101 3 0.29515638112850695 1101 4 0.16709465810695434 1101 5 0.055594442730978244 1101 6 0.3397527244598848 1101 7 0.18234803514561548 1101 8 0.3418771668246947 1101 9 0.47139068830453823 1101 10-11 0.5424668080789061 1101 12-13 0.6063623487621399 1101 14-15 0.7435839656590773 1101 16-17 0.5822666567968184 1101 18-19 0.5048185005390522 1101 20-21 0.6442454198245002 1101 22-23 0.7571639744915117 1101 24-25 0.6106301312105415 1101 26-27 0.6382744375326226 1101 28-29 0.6693410291068034 1101 30-31 0.6246788010481836 1101 32-33 0.6232598537098255 1101 34-35 0.577520146882236 1101 36-37 0.7221684751856827 1101 38-39 0.7218460057759657 1101 40-41 0.8104761120433679 1101 42-43 0.9353131139686681 1101 44-45 0.8532695819643337 1101 46-47 0.7828439382762227 1101 48-49 0.6314724977976596 1101 50-51 0.8506917677993684 1101 52-53 0.9804375964912211 1101 54-55 0.917124018466545 1101 56-57 0.9625499365395385 1101 58-59 1.1781689111626221 1101 60-61 1.0417313834726691 1101 62-63 0.9628266172537323 1101 64-65 0.8236029593364513 1101 66-67 0.8918396015495347 1101 68-69 0.8522240752828516 1101 70-71 0.9203177040918433 1101 72-73 0.8850886476986766 1101 74-75 1.0643457279575408 1101 76-77 1.2013847213510083 1101 78-79 1.4122429510169567 1101 80-81 1.1861989008919984 1101 82-83 1.2467761427862598 1101 84-85 1.1253077805448868 1101 86-87 1.3109905864700693 1101 88-89 1.5671763349734888 1101 90-91 1.5365703583117742 1101 92-93 1.269634369210511 1101 94-95 1.1609279905512047 1101 96-97 1.0423005775356273 1101 98-99 0.9728760494171382 1101 100-101 0.8884866609934701 1102 1 0.4611625527332315 1102 2 0.3880975465309717 1102 3 0.42155114586528697 1102 4 0.20955964534945792 1102 5 0.30671365495565794 1102 6 0.33835561459533636 1102 7 0.3718712941227622 1102 8 0.3279705591927069 1102 9 0.4505178786538693 1102 10-11 0.6543792363180003 1102 12-13 0.6692147042084642 1102 14-15 0.7991925145878227 1102 16-17 0.8964079530896427 1102 18-19 0.7621420414005584 1102 20-21 0.7697085825576053 1102 22-23 0.777927149419277 1102 24-25 0.5235895417392271 1102 26-27 0.48289506621447487 1102 28-29 0.5291595513948977 1102 30-31 0.5629412607119804 1102 32-33 0.6291959583588067 1102 34-35 0.49285399927052254 1102 36-37 0.6378813588485883 1102 38-39 0.5976286921667224 1102 40-41 0.616923337288199 1102 42-43 0.6126991642106745 1102 44-45 0.7230419990406176 1102 46-47 0.8365274304528754 1102 48-49 0.8263482177518853 1102 50-51 0.725772967848151 1102 52-53 0.6238210530219348 1102 54-55 0.4219244949965031 1102 56-57 0.4804634880948342 1102 58-59 0.5710335081758657 1102 60-61 0.48184209695434177 1102 62-63 0.6012381097868449 1102 64-65 0.8417246409806651 1102 66-67 0.27478518755005865 1102 68-69 0.5828750123959061 1102 70-71 0.8040066955931024 1102 72-73 0.6973169060191928 1102 74-75 0.7983856678284624 1102 76-77 0.8796217598298668 1102 78-79 0.9551839991952633 1102 80-81 1.0392162595276986 1102 82-83 1.0473177500430246 1102 84-85 0.9554237940152994 1102 86-87 0.9757226204462803 1102 88-89 0.8734072221822231 1102 90-91 0.7360017820857401 1102 92-93 0.8162575611170055 1102 94-95 1.141254713751085 1102 96-97 0.9955674577652616 1102 98-99 1.286898623616981 1102 100-101 1.250146694857115 1103 1 0.3291605622725946 1103 2 0.28466724977138824 1103 3 0.26692332488611115 1103 4 0.18791454587282175 1103 5 0.0879932334791036 1103 6 0.42634940194547255 1103 7 0.3339777257696923 1103 8 0.3746419893549131 1103 9 0.6585453739213065 1103 10-11 0.5781739404863728 1103 12-13 0.5465931654756773 1103 14-15 0.7518422283141177 1103 16-17 0.7901940852859255 1103 18-19 0.7000059732330826 1103 20-21 0.6098952404396485 1103 22-23 0.6599187004010574 1103 24-25 0.7380686349428984 1103 26-27 0.8195421607915208 1103 28-29 0.7631833789254259 1103 30-31 0.8652990170127879 1103 32-33 0.8392133342914008 1103 34-35 0.7592625401561577 1103 36-37 0.876165073725538 1103 38-39 0.9676913986364113 1103 40-41 1.0896090208188838 1103 42-43 0.9218150331465012 1103 44-45 1.077404019288565 1103 46-47 0.8957111560012976 1103 48-49 1.035873488250381 1103 50-51 1.3387318989383132 1103 52-53 1.1940601866843892 1103 54-55 1.2368882120077487 1103 56-57 1.4272955122401854 1103 58-59 1.3090484064721508 1103 60-61 1.370367601761135 1103 62-63 1.421618466399444 1103 64-65 1.670202553363282 1103 66-67 1.6555921476666136 1103 68-69 1.7161088613621693 1103 70-71 1.6771510782255206 1103 72-73 1.78494850144995 1103 74-75 1.7752224941340948 1103 76-77 1.8555277326037078 1103 78-79 1.779004454078155 1103 80-81 1.8448523021764522 1103 82-83 1.9053727359381263 1103 84-85 1.8358958208517215 1103 86-87 1.7665836106656485 1103 88-89 1.729545983735246 1103 90-91 1.9694998658613976 1103 92-93 1.6063959074107572 1103 94-95 1.7722701734593365 1103 96-97 1.7739495009336679 1103 98-99 2.024859004634763 1103 100-101 1.7602071626385616 1104 1 0.31187329038539957 1104 2 0.346712147181659 1104 3 0.28085911095350724 1104 4 0.2127189448793203 1104 5 0.2751103251081446 1104 6 0.3551905947530045 1104 7 0.4227639198355675 1104 8 0.4527068885397014 1104 9 0.578460152656838 1104 10-11 0.5540606528997571 1104 12-13 0.5670504262191614 1104 14-15 0.604581646664478 1104 16-17 0.7758242968714839 1104 18-19 0.7495024691279824 1104 20-21 0.7402573526904277 1104 22-23 0.8808411011720167 1104 24-25 0.7031274700923191 1104 26-27 0.5757649536852014 1104 28-29 0.7515773386618392 1104 30-31 0.6318868071358921 1104 32-33 0.5894076657826943 1104 34-35 0.6502039304480149 1104 36-37 0.7573405044215988 1104 38-39 0.6769664654457159 1104 40-41 0.8040021345925012 1104 42-43 0.8241096793069431 1104 44-45 0.8243997632475484 1104 46-47 0.6700747168796468 1104 48-49 0.5269056409745616 1104 50-51 0.793961578393187 1104 52-53 0.7293976873330834 1104 54-55 0.8884327358216595 1104 56-57 1.0020069725248533 1104 58-59 0.9113426620165157 1104 60-61 0.9711547768313586 1104 62-63 0.9466163598628157 1104 64-65 0.9789131241593729 1104 66-67 1.0935698501856947 1104 68-69 1.2858402458561606 1104 70-71 1.2353351543711 1104 72-73 1.3152079932807688 1104 74-75 1.296127010269604 1104 76-77 1.0888054589503682 1104 78-79 1.3367453204980642 1104 80-81 1.159392259688584 1104 82-83 1.3372166502235707 1104 84-85 1.4999916758014677 1104 86-87 1.4963653232492327 1104 88-89 1.2095981979423982 1104 90-91 1.2237620451307336 1104 92-93 1.417508069465086 1104 94-95 1.4608894628617541 1104 96-97 1.4224907452467193 1104 98-99 1.7944202200310606 1104 100-101 1.3674461990222166 1105 1 0.3660284407089307 1105 2 0.3324399397773554 1105 3 0.3479531246038974 1105 4 0.24323114347345154 1105 5 0.35430220685846336 1105 6 0.4498690369644649 1105 7 0.41019707132483774 1105 8 0.3484234804004984 1105 9 0.5293045054182102 1105 10-11 0.5726127766074427 1105 12-13 0.690333718859172 1105 14-15 0.7229527321150755 1105 16-17 0.48279212094742263 1105 18-19 0.5035378226226399 1105 20-21 0.4769479518625914 1105 22-23 0.5480780114882435 1105 24-25 0.6184495113658173 1105 26-27 0.558097741754942 1105 28-29 0.535740923111895 1105 30-31 0.6113279522431654 1105 32-33 0.6462190287536558 1105 34-35 0.67369418151101 1105 36-37 0.7803954847195911 1105 38-39 0.7799244532887499 1105 40-41 0.8033223010187669 1105 42-43 0.7147610947091678 1105 44-45 0.7504883430641982 1105 46-47 0.7658768587180163 1105 48-49 0.9675524901299468 1105 50-51 1.0249826449072899 1105 52-53 0.9661316658502344 1105 54-55 0.8076735569940041 1105 56-57 0.9793828310464079 1105 58-59 1.0534543362980813 1105 60-61 1.1269583422643272 1105 62-63 0.9558329262063552 1105 64-65 0.9943373687812738 1105 66-67 1.1934148663958695 1105 68-69 1.1589341735536713 1105 70-71 1.1743091455662977 1105 72-73 1.1568099938340914 1105 74-75 1.2010408290755628 1105 76-77 1.1221772024777152 1105 78-79 1.198635290065372 1105 80-81 1.343752729958286 1105 82-83 1.4493115636931897 1105 84-85 1.3685999374850155 1105 86-87 1.5695203580735786 1105 88-89 1.5975602282569028 1105 90-91 1.7795558065631383 1105 92-93 1.735092148842572 1105 94-95 1.658243725721782 1105 96-97 1.8472005871202768 1105 98-99 2.0914708274761935 1105 100-101 1.5515026109728893 1106 1 0.22144328493206444 1106 2 0.1857674412913859 1106 3 0.20399376789842805 1106 4 0.17191158220570202 1106 5 0.24842911621853858 1106 6 0.053934712221852976 1106 7 0.3464086317851027 1106 8 0.15559930975249614 1106 9 0.2727363067774746 1106 10-11 0.036542525614912336 1106 12-13 0.18484418248880985 1106 14-15 0.320257085792484 1106 16-17 0.2938546335643366 1106 18-19 0.0634489995286387 1106 20-21 0.3383085601662046 1106 22-23 0.3371689205791526 1106 24-25 0.2094723227513171 1106 26-27 0.17674722698473744 1106 28-29 0.37586675582839746 1106 30-31 0.1338163446797367 1106 32-33 0.22416936856928515 1106 34-35 -0.1227024270617676 1106 36-37 0.22410183018759255 1106 38-39 0.19341547004401605 1106 40-41 0.46231294767781606 1106 42-43 -0.10307417882559378 1106 44-45 0.07899517302180925 1106 46-47 0.16483184155901398 1106 48-49 0.4862336017644253 1106 50-51 0.22384072982755043 1106 52-53 0.31428791374741394 1106 54-55 0.33152928675647075 1106 56-57 -0.3958328341174422 1106 58-59 -0.10819090964768918 1106 60-61 0.6632048936404153 1106 62-63 0.5410829093837997 1106 64-65 0.6487442054679065 1106 66-67 0.37367191504670316 1106 68-69 0.3950427799731564 1106 70-71 0.21565764162982148 1106 72-73 0.5322638663864119 1106 74-75 0.6294076953698884 1106 76-77 0.5718548823108343 1106 78-79 0.6254255464411216 1106 80-81 0.9992557243731959 1106 82-83 0.8546853608804632 1106 84-85 0.7775730886861503 1106 86-87 0.6185695472263966 1106 88-89 0.5529400815642163 1106 90-91 0.5685787525290884 1106 92-93 0.5324436056758941 1106 94-95 0.6546167827165981 1106 96-97 0.7392388079667853 1106 98-99 0.7787159657575984 1106 100-101 0.4612563287576954 1107 1 0.2427770790806001 1107 2 0.17647979955748028 1107 3 0.11314476020192643 1107 4 0.02347308109575863 1107 5 0.11646564468495058 1107 6 0.21866799327037967 1107 7 0.23885888368263863 1107 8 0.10435763433476808 1107 9 0.34623907831504397 1107 10-11 0.4295161129283116 1107 12-13 0.4280631538961117 1107 14-15 0.5423528201502492 1107 16-17 0.6152293693710078 1107 18-19 0.47350748761169115 1107 20-21 0.4996337140297413 1107 22-23 0.5528886563193609 1107 24-25 0.536437854660555 1107 26-27 0.4314747921524287 1107 28-29 0.544255409140284 1107 30-31 0.7263265468624027 1107 32-33 0.8653279643235834 1107 34-35 0.6381285024999741 1107 36-37 0.7270962871124453 1107 38-39 0.8500590516415372 1107 40-41 0.7736531223444985 1107 42-43 0.9911674035429101 1107 44-45 0.8516638247385657 1107 46-47 0.8123231015784214 1107 48-49 0.9402682160753884 1107 50-51 0.7575063375054398 1107 52-53 0.9020765324981639 1107 54-55 0.9060221020271726 1107 56-57 0.9351621279317683 1107 58-59 0.9291974645165268 1107 60-61 0.8787914360642084 1107 62-63 1.0754287068821071 1107 64-65 1.1049212116756166 1107 66-67 1.1749526711381009 1107 68-69 1.0710408658177109 1107 70-71 1.2578505459619294 1107 72-73 1.4484201359101299 1107 74-75 1.3813837950123151 1107 76-77 1.3603953387837358 1107 78-79 1.2109474861186413 1107 80-81 1.4203404124589092 1107 82-83 1.3674518472088728 1107 84-85 1.4988906656843781 1107 86-87 1.4550298218545343 1107 88-89 1.4555899905089085 1107 90-91 1.2480962258438986 1107 92-93 1.4942387910432409 1107 94-95 1.6486290652140418 1107 96-97 1.7946759399238772 1107 98-99 1.7090526710175382 1107 100-101 1.6308657878758552 1108 1 0.2252843087730909 1108 2 0.15426245978640551 1108 3 0.26398944789410805 1108 4 0.15167803197215335 1108 5 0.19860218639160365 1108 6 0.21608094436808045 1108 7 0.25034712572359297 1108 8 0.4284526726058573 1108 9 0.45234372638488907 1108 10-11 0.37551179958418146 1108 12-13 0.3055203981650223 1108 14-15 0.4705998511352547 1108 16-17 0.46696800667771043 1108 18-19 0.41923159531123844 1108 20-21 0.2747873516449957 1108 22-23 0.34324433165456725 1108 24-25 0.4656754439544386 1108 26-27 0.48740143763895816 1108 28-29 0.42222256218420284 1108 30-31 0.4236710845344831 1108 32-33 0.3304525448524558 1108 34-35 0.4965236171642715 1108 36-37 0.6362487373344976 1108 38-39 0.3482010898296295 1108 40-41 0.268170738535602 1108 42-43 0.4011439003924835 1108 44-45 0.46096171998835445 1108 46-47 0.3759007726279435 1108 48-49 0.5771956127264275 1108 50-51 0.5084349194217381 1108 52-53 0.44510304456255056 1108 54-55 0.6113153115424907 1108 56-57 0.5562288879442789 1108 58-59 0.5717492852925048 1108 60-61 0.46283647827199914 1108 62-63 0.7712318145327046 1108 64-65 0.8038383005619991 1108 66-67 1.0376943790691655 1108 68-69 0.7435552134855925 1108 70-71 0.9195352505074297 1108 72-73 1.1029110570336016 1108 74-75 1.1033420043041957 1108 76-77 1.2400334604894105 1108 78-79 1.313492614508192 1108 80-81 1.2404325205499944 1108 82-83 1.3828424740375773 1108 84-85 1.2725645836776422 1108 86-87 0.9761230747799239 1108 88-89 1.1163894700136083 1108 90-91 1.0390376179879546 1108 92-93 1.3025463407786297 1108 94-95 1.218182271282089 1108 96-97 1.4617579104858862 1108 98-99 1.627820239861876 1108 100-101 1.4510509935523608 1109 1 0.2362720924881465 1109 2 0.230001994616849 1109 3 0.3535726738409686 1109 4 0.3100776585536025 1109 5 0.37384430708826955 1109 6 0.2801763493725815 1109 7 0.3406471205690451 1109 8 0.241376562802472 1109 9 0.3719728278321739 1109 10-11 0.3560781415141676 1109 12-13 0.4137225768217476 1109 14-15 0.377580974025463 1109 16-17 0.29810492872372407 1109 18-19 0.21211487910360916 1109 20-21 0.2451247742551459 1109 22-23 0.25413861754885403 1109 24-25 0.3292125824915786 1109 26-27 0.5284591514239381 1109 28-29 0.2878462914442963 1109 30-31 0.24558336553766935 1109 32-33 0.35792773141855605 1109 34-35 0.26324798616137457 1109 36-37 0.160890496067168 1109 38-39 0.22279760306251717 1109 40-41 0.4963310507868215 1109 42-43 0.49511551027318745 1109 44-45 0.1593808479529386 1109 46-47 0.39493022802103894 1109 48-49 0.7397873207726846 1109 50-51 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-2.217035303009432 2316 72-73 -2.3449185485141264 2316 74-75 -2.310032327694298 2316 76-77 -2.3612411491744574 2316 78-79 -2.425732154917924 2316 80-81 -2.624022473938556 2316 82-83 -2.7736530121559007 2316 84-85 -2.808314178409173 2316 86-87 -2.901665787908268 2316 88-89 -3.202568168574903 2316 90-91 -3.122930945826244 2316 92-93 -3.1390235848516994 2316 94-95 -3.551165354609161 2316 96-97 -3.373317226602671 2316 98-99 -3.219538799611259 2316 100-101 -2.9000649003487684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6556.0 3 2226.0 4 2372.0 5 2721.0 6 3162.0 7 3978.0 8 5127.0 9 6444.0 10 8253.0 11 10157.0 12 12382.0 13 14480.0 14 16828.0 15 19479.0 16 22970.0 17 26789.0 18 25400.0 19 22770.0 20 24236.0 21 26166.0 22 28746.0 23 31805.0 24 35634.0 25 39907.0 26 44601.0 27 50358.0 28 57332.0 29 65460.0 30 75447.0 31 87527.0 32 102892.0 33 122256.0 34 147585.0 35 181131.0 36 211962.0 37 210625.0 38 97595.0 39 4806.0 >>END_MODULE >>Per base sequence content fail #Base G A 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22.11657671964793 26.449779955048918 28.875388611496174 22.55825471380698 44-45 22.21845177370599 26.64053306308098 28.764626148368432 22.376389014844595 46-47 22.503697707313815 26.930666104026677 28.21773198672916 22.347904201930344 48-49 22.403066333438197 26.840240055823934 28.282770927313976 22.473922683423893 50-51 22.37086388908997 27.04540690162946 28.222606922834935 22.361122286445635 52-53 22.61195305109992 27.18493630843215 27.855369615616983 22.347741024850947 54-55 22.152593828118334 27.05910815553738 28.11311447483892 22.675183541505366 56-57 22.327394898640776 27.14760290801912 28.022866714354993 22.50213547898511 58-59 22.471381827009353 26.752952245719857 28.077136519463906 22.69852940780688 60-61 22.31437478619723 26.924009617121126 28.36593183557231 22.395683761109332 62-63 22.256190273714918 26.961121232753214 28.280259747948683 22.50242874558318 64-65 22.02648601358612 26.957165489011164 28.175761080667677 22.84058741673504 66-67 22.088049393732994 26.982212023508474 28.00720249024674 22.922536092511795 68-69 22.151173836779662 26.838555259592876 28.182900988015398 22.827369915612064 70-71 22.203042884347514 26.959282412236135 27.95957176214092 22.878102941275426 72-73 22.368373242251373 26.840890525349664 27.90639336695076 22.884342865448204 74-75 22.130741377050345 27.283234224847654 27.73073642454194 22.855287973560063 76-77 22.2922983136791 26.93120817336095 27.74776375089659 23.028729762063367 78-79 22.436199134960994 27.02058833739266 27.712463466727378 22.83074906091897 80-81 22.336639279767244 27.270532908949413 27.559247088101284 22.833580723182063 82-83 22.41325629539212 27.15646629751532 27.58842557945971 22.84185182763285 84-85 22.44523804205936 27.02398113736095 27.554667773912044 22.97611304666765 86-87 22.44404758444668 27.06561861180095 27.48035704482114 23.009976758931234 88-89 22.456380442114884 27.011770241526023 27.384021693896194 23.147827622462895 90-91 22.435763537463018 27.03761806114846 27.43403541942125 23.092582981967276 92-93 22.66660745119097 26.849777067192072 27.42446325881604 23.05915222280092 94-95 22.510542116779515 26.722543116073123 27.465444685653246 23.301470081494116 96-97 22.537375427579416 26.662192679188983 27.441129512246533 23.35930238098507 98-99 22.58118606368708 26.75872634450624 27.01791670165602 23.64217089015066 100-101 23.153352710642064 26.141971369191587 27.30506766709159 23.39960825307476 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 981.0 1 666.5 2 603.0 3 817.0 4 636.5 5 567.0 6 868.5 7 1865.0 8 3010.5 9 3364.5 10 2890.5 11 2332.5 12 2241.0 13 2356.0 14 2491.0 15 2597.0 16 2706.5 17 2807.5 18 2984.0 19 3172.0 20 3439.5 21 3868.5 22 4444.5 23 5219.5 24 6064.5 25 7185.0 26 8783.5 27 11003.0 28 13266.5 29 16163.0 30 19828.0 31 22970.5 32 26573.0 33 30299.0 34 33841.5 35 37415.0 36 41871.0 37 46047.5 38 48667.5 39 50992.5 40 53385.5 41 56706.0 42 60104.5 43 62966.0 44 65320.5 45 68091.5 46 70097.5 47 72204.0 48 77026.0 49 76864.0 50 71705.5 51 67393.5 52 64466.5 53 62897.5 54 61256.0 55 57786.0 56 52290.0 57 47206.0 58 43188.5 59 38798.0 60 32701.5 61 26711.5 62 21606.0 63 16566.0 64 12516.5 65 9672.5 66 7395.5 67 5667.0 68 4296.0 69 3170.5 70 2415.5 71 1889.0 72 1572.0 73 1353.5 74 1062.5 75 795.0 76 572.5 77 381.0 78 235.5 79 150.5 80 97.5 81 53.5 82 31.5 83 23.0 84 16.5 85 10.0 86 6.0 87 7.0 88 5.0 89 4.5 90 5.0 91 1.5 92 2.0 93 4.0 94 3.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006619433688612152 2 0.10408117685996669 3 0.11392960259180428 4 0.11586699781773954 5 0.15477635193860612 6 0.09417893459407534 7 0.027554065435523755 8 0.026423918220394853 9 0.023302559245276924 10-11 0.05064135854458565 12-13 0.05408561672402612 14-15 0.026962083560932423 16-17 0.031590305489555555 18-19 0.037617757303576374 20-21 0.0590367378569718 22-23 0.032370645233335035 24-25 0.04891922945486542 26-27 0.05769132450562786 28-29 0.05403180018997237 30-31 0.06794337424286864 32-33 0.05018391800512872 34-35 0.06221191336614348 36-37 0.05271329510565531 38-39 0.031025231881991102 40-41 0.03581490341277551 42-43 0.03175175509171683 44-45 0.007991755306982965 46-47 0.013830849251815635 48-49 0.009713884396703199 50-51 0.008314654511305508 52-53 0.0100906001350795 54-55 0.01111311428210089 56-57 0.014422831126406965 58-59 0.018405254646385007 60-61 0.023490917114465076 62-63 0.01130147215128904 64-65 0.00890663638589684 66-67 0.010574948941563316 68-69 0.010655673742643952 70-71 0.02973363506470093 72-73 0.026773725691744278 74-75 0.028011505974980692 76-77 0.022737485637712475 78-79 0.005300928604295097 80-81 0.005327836871321976 82-83 0.006431075819424001 84-85 0.005408561672402613 86-87 0.013292683911278063 88-89 0.036810509292770015 90-91 0.03514219673710354 92-93 0.029195469724163356 94-95 0.04590550354785501 96-97 0.04098129068193621 98-99 0.0245941560625671 100-101 0.07286758710878742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1858165.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.37541777642965 #Duplication Level Percentage of deduplicated Percentage of total 1 78.78668877980942 45.20419183963107 2 12.042521916297243 13.81889452058729 3 3.8244990520853346 6.582966926968657 4 1.7106645652561032 3.9260037642761327 5 0.9339839084263276 2.6793858471211576 6 0.5650704080680783 1.9452690441602143 7 0.36874139115520993 1.4809683958294422 8 0.27671599128503516 1.2701356484318207 9 0.20972130295693153 1.0829562636394006 >10 1.1527753435183652 12.071615953177817 >50 0.08813293747551267 3.5348013853589424 >100 0.03691577430822126 3.783067566767137 >500 0.0028173389670089967 1.0975763416972004 >1k 5.634677934017992E-4 0.7140981471042218 >5k 1.8782259780059972E-4 0.8080683552495295 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8302 0.4467848657142934 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6695 0.36030169548990537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4806 0.25864226266235774 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2616 0.14078405308462918 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.3816534053757334E-5 0.0 10-11 0.0 0.0 0.0 5.3816534053757334E-5 0.0 12-13 0.0 0.0 0.0 5.3816534053757334E-5 0.0 14-15 0.0 0.0 0.0 1.0763306810751467E-4 0.0 16-17 0.0 0.0 0.0 1.0763306810751467E-4 0.0 18-19 0.0 0.0 0.0 1.61449602161272E-4 0.0 20-21 0.0 0.0 0.0 2.690826702687867E-4 0.0 22-23 0.0 0.0 0.0 5.381653405375734E-4 0.0 24-25 0.0 0.0 0.0 9.956058799945107E-4 0.0 26-27 0.0 0.0 0.0 0.0017759456237739922 0.0 28-29 0.0 0.0 0.0 0.004762763263757524 0.0 30-31 0.0 0.0 0.0 0.013454133513439333 0.0 32-33 0.0 0.0 0.0 0.03368915031765209 0.0 34-35 0.0 0.0 0.0 0.06062432561155764 0.0 36-37 0.0 0.0 0.0 0.1053189571432031 0.0 38-39 0.0 0.0 0.0 0.17398885459579747 0.0 40-41 0.0 0.0 0.0 0.24744842357917624 0.0 42-43 0.0 0.0 0.0 0.3235450027311891 0.0 44-45 0.0 0.0 0.0 0.4201725896247104 0.0 46-47 0.0 0.0 0.0 0.5323531548597675 0.0 48-49 0.0 0.0 0.0 0.654247604491528 0.0 50-51 0.0 0.0 0.0 0.7867708195989054 0.0 52-53 0.0 0.0 0.0 0.91724900641224 0.0 54-55 0.0 0.0 0.0 1.0608315192676647 2.6908267026878667E-5 56-57 0.0 0.0 0.0 1.232613895967258 5.3816534053757334E-5 58-59 0.0 0.0 0.0 1.418872920327312 5.3816534053757334E-5 60-61 0.0 0.0 0.0 1.6269814575131918 5.3816534053757334E-5 62-63 0.0 0.0 0.0 1.8294123503564 5.3816534053757334E-5 64-65 0.0 0.0 0.0 2.0415302193292844 5.3816534053757334E-5 66-67 0.0 0.0 0.0 2.2832471820317357 5.3816534053757334E-5 68-69 0.0 0.0 0.0 2.549908108268103 5.3816534053757334E-5 70-71 0.0 0.0 0.0 2.8420780716459517 5.3816534053757334E-5 72-73 0.0 0.0 0.0 3.1357818062443323 5.3816534053757334E-5 74-75 0.0 0.0 0.0 3.434625019844847 5.3816534053757334E-5 76-77 0.0 0.0 0.0 3.7531650849090363 5.3816534053757334E-5 78-79 0.0 0.0 0.0 4.111879192644356 5.3816534053757334E-5 80-81 0.0 0.0 0.0 4.497097943401151 5.3816534053757334E-5 82-83 0.0 0.0 0.0 4.897492956761106 5.3816534053757334E-5 84-85 0.0 0.0 0.0 5.2956814922248565 5.3816534053757334E-5 86-87 0.0 0.0 0.0 5.702938113676665 5.3816534053757334E-5 88-89 0.0 0.0 0.0 6.153086512769319 5.3816534053757334E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11315 0.0 30.93468 1 GGTATCA 7680 0.0 28.794806 1 GTACATG 10050 0.0 25.790087 1 TATCAAC 13585 0.0 25.451235 2 TACATGG 10010 0.0 25.371216 2 ATCAACG 13670 0.0 25.292978 3 TCAACGC 13855 0.0 24.989578 4 CAACGCA 14000 0.0 24.764729 5 AACGCAG 14370 0.0 24.175678 6 ACATGGG 10415 0.0 23.197357 3 GAGTACT 7150 0.0 22.494541 12-13 ACGCAGA 15830 0.0 21.885324 7 CGCAGAG 15865 0.0 21.777132 8 GTACTTT 7725 0.0 20.630112 14-15 AGTACTT 7600 0.0 20.568697 12-13 GCAGAGT 16365 0.0 20.413725 9 CATGGGA 5895 0.0 20.088598 4 CATGGGG 4260 0.0 19.872133 4 AGAGTAC 13270 0.0 19.731129 10-11 TACCTGG 1355 0.0 19.304453 2 >>END_MODULE