FastQCFastQC Report
Thu 26 May 2016
SRR938395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858165
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80440.43290019992842405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67910.36546808275906606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50720.27295746072065724No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25770.13868520825653266No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78000.031.0787491
GTATCAA115400.029.9032751
TATCAAC140000.024.7506962
ATCAACG139950.024.7488733
TCAACGC142150.024.332424
CAACGCA144450.023.977885
GTACATG105350.023.777381
TACATGG105050.023.5738012
AACGCAG147700.023.5000516
TAGCCCT11200.023.3316524
GAGTACT70800.023.30523712-13
ACATGGG106950.022.212123
GTACTTT75300.021.66026114-15
TACCTGG14750.021.2686462
ACGCAGA164300.021.0101327
CGCAGAG164300.020.9234338
AGTACTT74250.020.84745812-13
GTGTAGC10350.020.6661761
GTACCTG16650.020.5544661
CGCGGAA1452.4265319E-919.6658582-83
Produced by FastQC (version 0.11.5)