##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938395_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1858165 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.804319315023154 31.0 31.0 34.0 30.0 34.0 2 32.02592934427244 31.0 31.0 34.0 30.0 34.0 3 32.08243347603684 33.0 31.0 34.0 30.0 34.0 4 35.75438187674399 37.0 35.0 37.0 33.0 37.0 5 35.65446609961979 37.0 35.0 37.0 33.0 37.0 6 35.66305252762806 37.0 35.0 37.0 33.0 37.0 7 35.60452758500994 37.0 35.0 37.0 33.0 37.0 8 35.59056865240708 37.0 35.0 37.0 33.0 37.0 9 37.166448620009525 39.0 37.0 39.0 33.0 39.0 10-11 37.068820314665274 39.0 37.0 39.0 33.0 39.0 12-13 36.958063465838606 39.0 37.0 39.0 33.0 39.0 14-15 37.97167716537552 40.0 37.0 41.0 33.0 41.0 16-17 37.88884383249066 40.0 37.0 41.0 33.0 41.0 18-19 38.15495206292229 40.0 37.0 41.0 33.0 41.0 20-21 38.13832302298235 40.0 37.0 41.0 33.0 41.0 22-23 38.026892660232 40.0 37.0 41.0 33.0 41.0 24-25 37.9004859633025 40.0 37.0 41.0 32.5 41.0 26-27 37.70141214585357 40.0 37.0 41.0 32.0 41.0 28-29 37.59052425376648 39.5 37.0 41.0 32.0 41.0 30-31 37.34136284990838 39.0 36.0 41.0 31.5 41.0 32-33 37.138644307690655 39.0 36.0 41.0 31.0 41.0 34-35 37.02805698094626 39.0 36.0 41.0 31.0 41.0 36-37 36.98308815417361 39.0 36.0 41.0 31.0 41.0 38-39 36.825166763984896 39.0 35.0 41.0 30.0 41.0 40-41 36.6640812306765 39.0 35.0 41.0 30.0 41.0 42-43 36.44439944784236 39.0 35.0 40.0 30.0 41.0 44-45 36.11756760029384 39.0 35.0 40.0 29.5 41.0 46-47 35.702105571894855 38.0 35.0 40.0 28.5 41.0 48-49 35.32286207091405 38.0 34.0 40.0 27.0 41.0 50-51 34.95569042576951 38.0 34.0 40.0 26.0 41.0 52-53 34.47425524643936 38.0 33.0 40.0 24.0 41.0 54-55 34.14057659034585 38.0 33.0 40.0 24.0 41.0 56-57 33.81081604701413 37.0 33.0 40.0 23.0 41.0 58-59 33.34719333320776 37.0 32.0 39.5 22.0 41.0 60-61 32.933468771610706 36.0 31.5 39.0 21.5 40.0 62-63 32.45515360584233 36.0 31.0 39.0 19.5 40.0 64-65 31.921796234457112 35.0 30.5 38.5 18.0 40.0 66-67 31.40432469667656 35.0 30.0 38.0 14.5 40.0 68-69 30.90146246431291 34.5 29.0 37.5 12.0 40.0 70-71 30.316110786717005 34.0 29.0 37.0 8.0 39.0 72-73 29.80518495397341 34.0 28.0 36.0 7.0 39.0 74-75 29.203583912085307 34.0 27.5 36.0 4.5 38.5 76-77 27.975888578247897 31.5 26.0 34.5 2.0 36.5 78-79 28.661559656973413 33.0 27.5 35.0 2.0 37.0 80-81 28.258599209435115 33.0 26.5 35.0 2.0 37.0 82-83 27.80486447651312 33.0 26.0 35.0 2.0 36.0 84-85 27.23694209071853 32.5 26.0 35.0 2.0 36.0 86-87 26.604490451601443 32.0 25.0 34.5 2.0 35.0 88-89 25.847728269556256 31.5 23.5 34.0 2.0 35.0 90-91 25.21148095029236 31.0 20.5 34.0 2.0 35.0 92-93 24.364806677555546 31.0 17.5 34.0 2.0 35.0 94-95 23.538669870544325 30.5 4.5 34.0 2.0 35.0 96-97 22.454533908452696 30.0 2.0 34.0 2.0 35.0 98-99 21.077179905982515 29.0 2.0 34.0 2.0 35.0 100-101 17.125465714831567 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27358216168646265 1101 2 0.30467412002669647 1101 3 0.24768600885879266 1101 4 0.12929848377697795 1101 5 0.12721746448898585 1101 6 0.26372575730626835 1101 7 0.15842292805915292 1101 8 0.3032160324542801 1101 9 0.2774404792591909 1101 10-11 0.3037511945078393 1101 12-13 0.34524561741989857 1101 14-15 0.38048601925327574 1101 16-17 0.42322234843990714 1101 18-19 0.4724225491302718 1101 20-21 0.3469094827671668 1101 22-23 0.3403263562258516 1101 24-25 0.4038948680208563 1101 26-27 0.2961854536429982 1101 28-29 0.22164400720595268 1101 30-31 0.4358101891504873 1101 32-33 0.3804316818237581 1101 34-35 0.34841982309065855 1101 36-37 0.35985312523064295 1101 38-39 0.46684532212272956 1101 40-41 0.44046353519811277 1101 42-43 0.504263518732408 1101 44-45 0.4889800674611351 1101 46-47 0.2986748359026805 1101 48-49 0.43635091611594845 1101 50-51 0.44627401752993734 1101 52-53 0.3914044082031225 1101 54-55 0.3248375086318731 1101 56-57 0.3297381797410779 1101 58-59 0.52587651982099 1101 60-61 0.49958019520865804 1101 62-63 0.702347234930663 1101 64-65 0.590638834217863 1101 66-67 0.18300657084531124 1101 68-69 0.528046935274574 1101 70-71 0.6848669632721673 1101 72-73 0.5597832097075539 1101 74-75 0.375284365994613 1101 76-77 0.21502049687574853 1101 78-79 0.515303538228423 1101 80-81 0.43585176258167024 1101 82-83 0.3847796966051682 1101 84-85 0.5225022116460458 1101 86-87 0.6551464203639483 1101 88-89 0.6147025474299923 1101 90-91 0.6397999653403303 1101 92-93 0.883518922193332 1101 94-95 0.7483098961228798 1101 96-97 0.7874596202336903 1101 98-99 0.9883396541252587 1101 100-101 1.5191437165455604 1102 1 0.2485608210238226 1102 2 0.27608466747332017 1102 3 0.12593283824605805 1102 4 0.10997990755094378 1102 5 0.09160200915897576 1102 6 0.14645295328390517 1102 7 0.13272736316477562 1102 8 0.2993852662541343 1102 9 0.16810182873950907 1102 10-11 0.25491244314765993 1102 12-13 0.3582141739468554 1102 14-15 0.45643327498476793 1102 16-17 0.467013212291171 1102 18-19 0.3619371311346953 1102 20-21 0.3006471473951322 1102 22-23 0.3235141352960511 1102 24-25 0.34624195396510515 1102 26-27 0.3796034070031027 1102 28-29 0.34971641771587514 1102 30-31 0.34807983897601247 1102 32-33 0.3153359185315736 1102 34-35 0.3742490602674948 1102 36-37 0.536066025309438 1102 38-39 0.49063195301838647 1102 40-41 0.4465922334645924 1102 42-43 0.34918051293524144 1102 44-45 0.3778553090092913 1102 46-47 0.5241386435459248 1102 48-49 0.4634623518969718 1102 50-51 0.5461556472061204 1102 52-53 0.5328203025410474 1102 54-55 0.5795435937693938 1102 56-57 0.4695881150155543 1102 58-59 0.5235721386533001 1102 60-61 0.4676111204084563 1102 62-63 0.591732541767179 1102 64-65 0.6583500012472427 1102 66-67 0.6295218282465349 1102 68-69 0.778421569434645 1102 70-71 0.5247952844421029 1102 72-73 0.6286707514508301 1102 74-75 0.7862970273394048 1102 76-77 0.6128101553313421 1102 78-79 0.7282766091263255 1102 80-81 0.9050344386538605 1102 82-83 0.8221894091863113 1102 84-85 0.8142349327446929 1102 86-87 0.7941497645161384 1102 88-89 0.7646080974093543 1102 90-91 0.6756437774862185 1102 92-93 1.1708776868738369 1102 94-95 1.0585700059686651 1102 96-97 0.9350015745693625 1102 98-99 1.2592661085734989 1102 100-101 1.3883239888617993 1103 1 0.22320564675412413 1103 2 0.20363236429229659 1103 3 0.17683689711544304 1103 4 0.10905253384543556 1103 5 0.04020497489892705 1103 6 0.13051302900492345 1103 7 0.22720000709039567 1103 8 0.14880274333368249 1103 9 0.2576267396559544 1103 10-11 0.2844384792079424 1103 12-13 0.2295116387774101 1103 14-15 0.5052068102098914 1103 16-17 0.4685075076959464 1103 18-19 0.3976940861475029 1103 20-21 0.4053875987517941 1103 22-23 0.3414306009060937 1103 24-25 0.47137853714686884 1103 26-27 0.41394871248544973 1103 28-29 0.47113712639410465 1103 30-31 0.3723443776902329 1103 32-33 0.3584141380668555 1103 34-35 0.2879540081165004 1103 36-37 0.4364594776683006 1103 38-39 0.5236555044826972 1103 40-41 0.6412917040670223 1103 42-43 0.6954583167472848 1103 44-45 0.6334917576377777 1103 46-47 0.6218483958750838 1103 48-49 0.7005052187170548 1103 50-51 0.8828128912764939 1103 52-53 0.679209634785586 1103 54-55 0.6546631837994568 1103 56-57 0.7437804473343235 1103 58-59 0.887539639395662 1103 60-61 1.0686301875366269 1103 62-63 1.097873565207287 1103 64-65 0.9300551277273286 1103 66-67 1.0732304183137238 1103 68-69 1.06640677321327 1103 70-71 0.9208177849988779 1103 72-73 1.0848730778306965 1103 74-75 0.9964956442244493 1103 76-77 0.8958287770417463 1103 78-79 1.1772626457433795 1103 80-81 1.1871393159783885 1103 82-83 1.3691311058568125 1103 84-85 1.4037998616634404 1103 86-87 1.278391371175136 1103 88-89 1.3212715587693644 1103 90-91 1.487142457472114 1103 92-93 1.632106332339852 1103 94-95 1.7463620213912563 1103 96-97 1.9302881498635251 1103 98-99 2.1905520087813635 1103 100-101 2.3162066189609014 1104 1 0.1879014879765073 1104 2 0.29915473236311385 1104 3 0.262615347496876 1104 4 0.16602360760993662 1104 5 0.01094225155721773 1104 6 0.1677086065614688 1104 7 0.22933249178242932 1104 8 0.24699804132072245 1104 9 0.283089326728458 1104 10-11 0.26534125989208235 1104 12-13 0.3354200694438987 1104 14-15 0.40870120570642854 1104 16-17 0.38670664488086004 1104 18-19 0.40531681910952955 1104 20-21 0.34441759304322517 1104 22-23 0.3504923355427394 1104 24-25 0.35862539701223994 1104 26-27 0.3138675759489473 1104 28-29 0.32905192856659227 1104 30-31 0.3332007420256531 1104 32-33 0.31271052414284384 1104 34-35 0.4460367472949045 1104 36-37 0.46736864771518327 1104 38-39 0.6306310145296194 1104 40-41 0.6837275356132864 1104 42-43 0.7074085176634455 1104 44-45 0.4659823368153795 1104 46-47 0.39888321260082193 1104 48-49 0.501842911325646 1104 50-51 0.5626586648943999 1104 52-53 0.5015529851888303 1104 54-55 0.7228226176516941 1104 56-57 0.6838967727978584 1104 58-59 0.6747820908797522 1104 60-61 0.7937179505956209 1104 62-63 0.7522902331295 1104 64-65 0.6755747614857057 1104 66-67 0.6729305646831385 1104 68-69 0.7869972898364956 1104 70-71 0.8214816090966508 1104 72-73 0.8318968624427683 1104 74-75 1.1241869191450569 1104 76-77 1.1057981639028718 1104 78-79 1.19063329407118 1104 80-81 1.1037158619391114 1104 82-83 1.0750121375966017 1104 84-85 1.1992918453847956 1104 86-87 1.3776453781984124 1104 88-89 1.376321917550218 1104 90-91 1.4292238607435799 1104 92-93 1.4273022848878156 1104 94-95 1.4424430541354845 1104 96-97 1.340315040989342 1104 98-99 1.4534163907904762 1104 100-101 1.8591463452283286 1105 1 0.2174381213632941 1105 2 0.28862040090194085 1105 3 0.21062218075097405 1105 4 0.16658445016305734 1105 5 0.112669098671212 1105 6 0.16727884215294608 1105 7 0.16258040943957042 1105 8 0.1351644783320367 1105 9 0.2310445882535319 1105 10-11 0.23796781879532602 1105 12-13 0.22671962329471995 1105 14-15 0.34399050430405254 1105 16-17 0.2901877365898997 1105 18-19 0.2938789079794333 1105 20-21 0.3511585820922889 1105 22-23 0.1720557647403993 1105 24-25 0.3043453464074801 1105 26-27 0.1211072260132795 1105 28-29 0.16743042745323322 1105 30-31 0.4235150609699332 1105 32-33 0.46997596007168596 1105 34-35 0.4444875809035551 1105 36-37 0.38033974337268717 1105 38-39 0.3073350975601059 1105 40-41 0.35037969773116373 1105 42-43 0.43322916623165497 1105 44-45 0.3376912144050408 1105 46-47 0.22965917910068612 1105 48-49 0.3597053168782125 1105 50-51 0.3002172538704073 1105 52-53 0.3632249836201922 1105 54-55 0.15268936382477705 1105 56-57 0.03416121221047774 1105 58-59 0.15611607960381235 1105 60-61 0.21069753947224257 1105 62-63 0.15736507928727406 1105 64-65 0.284329599692839 1105 66-67 0.35060510946862067 1105 68-69 0.47911111294009245 1105 70-71 0.4170040011653491 1105 72-73 0.22871485990178542 1105 74-75 0.46962040370174307 1105 76-77 0.3969542838823479 1105 78-79 0.667392709269258 1105 80-81 0.5970457840174497 1105 82-83 0.8268805383626585 1105 84-85 0.8526963853085654 1105 86-87 0.5649650450235519 1105 88-89 0.6699896075933829 1105 90-91 0.8892646346418402 1105 92-93 0.9667970968255943 1105 94-95 0.9010310900315552 1105 96-97 0.9969035979747574 1105 98-99 1.1243855363919728 1105 100-101 1.0122718068893128 1106 1 0.0013961322643076812 1106 2 0.1367261811320759 1106 3 0.12982950192720466 1106 4 0.0884497351371536 1106 5 0.05544549326108239 1106 6 -0.025373065524874505 1106 7 0.04041097265873361 1106 8 0.1308188757480906 1106 9 -0.19961956624482013 1106 10-11 0.13174280029051744 1106 12-13 0.034208352182410806 1106 14-15 0.0331513952266036 1106 16-17 0.13170714981916376 1106 18-19 0.04105742894034137 1106 20-21 0.24751347072697172 1106 22-23 0.24453406364357022 1106 24-25 0.2030627947622321 1106 26-27 0.12561297932732884 1106 28-29 0.1938404940705567 1106 30-31 0.17827786293481296 1106 32-33 0.12694991875549277 1106 34-35 0.19069106653709156 1106 36-37 0.17093391603939523 1106 38-39 0.12639438024633165 1106 40-41 0.08291990363924384 1106 42-43 0.21918233223663464 1106 44-45 0.09398621474149849 1106 46-47 -0.5089559881155239 1106 48-49 -0.2237143722409627 1106 50-51 0.25463149248152916 1106 52-53 0.22617229537579675 1106 54-55 0.45940896546665755 1106 56-57 0.2651739300625735 1106 58-59 0.09769201662016513 1106 60-61 0.18016863832158236 1106 62-63 -0.24468525379932515 1106 64-65 0.13137354020827985 1106 66-67 0.08739940325773077 1106 68-69 -0.06267476957117779 1106 70-71 -0.10211988013469764 1106 72-73 0.410372218087641 1106 74-75 0.49263029536448144 1106 76-77 0.41113569832283403 1106 78-79 0.5482526783157589 1106 80-81 -0.03083322816207712 1106 82-83 0.5197269147841617 1106 84-85 0.6012227294307628 1106 86-87 0.8310958572165426 1106 88-89 0.799639025532958 1106 90-91 0.8751902116555001 1106 92-93 1.0928088053243492 1106 94-95 1.1311185673211064 1106 96-97 1.011554698169899 1106 98-99 0.997633635443183 1106 100-101 0.7497289093723225 1107 1 0.08510853040863964 1107 2 0.17004444935310659 1107 3 0.07547599463613608 1107 4 -0.013576876940639693 1107 5 -0.13014251136479515 1107 6 -0.06450026381276786 1107 7 0.06874499278394808 1107 8 0.05561144244444449 1107 9 0.12896515507276263 1107 10-11 0.13163167293271272 1107 12-13 0.08347711821678416 1107 14-15 0.2342941726129837 1107 16-17 0.23238866891419008 1107 18-19 0.07912479657782256 1107 20-21 0.19690107391190992 1107 22-23 0.263202396334421 1107 24-25 0.28006222317414853 1107 26-27 0.2528712175067511 1107 28-29 0.2639355372055192 1107 30-31 0.25882299845435597 1107 32-33 0.22522704592719123 1107 34-35 0.29544960380330565 1107 36-37 0.2830571663816883 1107 38-39 0.16874360727313586 1107 40-41 0.39763071994678967 1107 42-43 0.3992438776513083 1107 44-45 0.3080771902961317 1107 46-47 0.4385149340022778 1107 48-49 0.5327339389156336 1107 50-51 0.5886064223622967 1107 52-53 0.6797382074575751 1107 54-55 0.5603714932152641 1107 56-57 0.4683180633090629 1107 58-59 0.4252247500075512 1107 60-61 0.4727175223955413 1107 62-63 0.6621476165033471 1107 64-65 0.600559891216534 1107 66-67 0.4760507212335092 1107 68-69 0.8906267497897069 1107 70-71 0.9957167254296451 1107 72-73 1.022771159985048 1107 74-75 0.7596350408748584 1107 76-77 1.0486245656479305 1107 78-79 1.2618419856307561 1107 80-81 1.2911103042522818 1107 82-83 1.1997052476079375 1107 84-85 1.2275769279446322 1107 86-87 1.4378674479594729 1107 88-89 1.3926812034164868 1107 90-91 1.3030556624789824 1107 92-93 1.609044698397497 1107 94-95 1.6895631830942754 1107 96-97 1.5662095520161543 1107 98-99 1.678217452854021 1107 100-101 1.8511133488836826 1108 1 0.18112991199808803 1108 2 0.12485966926556102 1108 3 0.06133367343137763 1108 4 0.05250378919120635 1108 5 0.03034761816320497 1108 6 0.0409660008141941 1108 7 0.014237686485444101 1108 8 0.0272443421735602 1108 9 0.18445716783191557 1108 10-11 0.030234038781756567 1108 12-13 0.2028625358365943 1108 14-15 0.22598720806241346 1108 16-17 0.27335293646494563 1108 18-19 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-1.2476089127707723 2316 78-79 -1.4135663080669865 2316 80-81 -1.428801434452435 2316 82-83 -1.559036335388484 2316 84-85 -1.5980410939941336 2316 86-87 -1.4975659507741277 2316 88-89 -1.444416784428892 2316 90-91 -1.4422115811825371 2316 92-93 -1.8291252337908972 2316 94-95 -1.7676244346433734 2316 96-97 -2.161632651393287 2316 98-99 -2.150732495070603 2316 100-101 -2.524303769358344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 34.0 5 67.0 6 155.0 7 379.0 8 832.0 9 1442.0 10 2450.0 11 3754.0 12 5208.0 13 7584.0 14 9851.0 15 13399.0 16 17186.0 17 21429.0 18 21031.0 19 17107.0 20 16953.0 21 18190.0 22 20154.0 23 22107.0 24 25750.0 25 30050.0 26 35046.0 27 42175.0 28 50505.0 29 59416.0 30 70890.0 31 83136.0 32 100464.0 33 122038.0 34 153653.0 35 200053.0 36 258728.0 37 287289.0 38 134650.0 39 4997.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78236834542595 19.73066875906139 15.370544377819323 22.11641851769333 2 15.821997946411791 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23.044966405028617 26.222832740902984 29.306170334711933 21.426030519356463 22-23 22.636579636361677 26.213387939176553 29.186670720845566 21.9633617036162 24-25 22.423543411400722 26.41526798657611 29.381471735005572 21.779716867017594 26-27 22.07780046476634 26.589655881317746 29.467321587524715 21.865222066391194 28-29 21.848663519474062 26.839988289515887 29.491207449934453 21.8201407410756 30-31 22.043245265045766 26.467657541416134 29.404062433989147 22.085034759548954 32-33 21.924884751458166 26.519650105104088 29.233251424259937 22.322213719177807 34-35 22.50484407462028 26.56921106171351 29.087510899167896 21.838433964498314 36-37 22.49994213358583 26.27127129013036 29.158213235852198 22.07057334043161 38-39 22.140881227497054 26.58689837413688 29.211609771324316 22.060610627041747 40-41 22.288192944855023 26.53077863840196 29.12879628770052 22.0522321290425 42-43 22.109630841511294 26.542788641360065 28.88598575461011 22.461594762518533 44-45 22.19601112276348 26.653835606890556 28.789152157047006 22.361001113298958 46-47 22.47830520970958 26.921182995051566 28.29320862248509 22.307303172753766 48-49 22.33439979764983 26.841776699055252 28.287611702943494 22.53621180035142 50-51 22.398619058446496 27.028486628519456 28.258983049023158 22.313911264010894 52-53 22.624955265006065 27.18832827009442 27.819865297215262 22.36685116768425 54-55 22.235862021086316 27.049129377498655 28.041931528269497 22.673077073145528 56-57 22.277542629422037 27.17586974246098 27.989145205081357 22.557442423035628 58-59 22.52474114984107 26.78608628248916 27.942333002285043 22.74683956538473 60-61 22.328399254102838 26.962514093204852 28.30507516824394 22.404011484448365 62-63 22.26351868661276 27.064634094816693 28.169197155289265 22.502650063281276 64-65 22.02569547851834 27.040617091941844 28.0578542534201 22.875833176119713 66-67 22.041700842391247 27.060446361873026 27.8790760606315 23.018776735104225 68-69 22.140496490548163 26.868810015932482 28.022811436937516 22.967882056581836 70-71 22.119486320491347 26.972963882329992 27.86155677988335 23.045993017295313 72-73 22.226058148273818 26.909069088914944 27.79540121200314 23.069471550808096 74-75 21.968126351271152 27.308428002496584 27.584973474511926 23.13847217172034 76-77 21.971535604723694 26.990401040257478 27.858278379255154 23.17978497576367 78-79 22.140200643473865 27.01489335415261 27.818522389558204 23.02638361281532 80-81 22.04296155218848 27.222862513666378 27.71948164923525 23.014694284909893 82-83 22.054177354347182 27.143671228687783 27.742737648747074 23.059413768217958 84-85 22.01611114819113 26.98054842064242 27.699984498160234 23.303355933006216 86-87 21.92081525470116 27.10011967352567 27.629864074879514 23.349200996893654 88-89 21.915281136292453 27.06771169549491 27.444071809779114 23.572935358433522 90-91 21.88384924102934 27.008384918719873 27.635214989875816 23.472550850374972 92-93 21.940078005123006 26.87225618150631 27.634570410645214 23.553095402725468 94-95 21.720933111907218 26.68129981078295 27.67968217995268 23.918084897357154 96-97 21.638856900434522 26.699798270846824 27.69188900212712 23.96945582659153 98-99 21.588248327895386 26.75846606024073 27.37359702191323 24.279688589950656 100-101 21.621476877085872 26.45707905192271 27.71818945882862 24.2032546121628 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1124.0 1 762.0 2 591.5 3 761.5 4 602.5 5 535.0 6 921.5 7 1993.5 8 2936.0 9 3183.0 10 2793.0 11 2224.5 12 2163.0 13 2307.0 14 2465.5 15 2584.0 16 2754.5 17 2871.5 18 2959.5 19 3170.5 20 3448.0 21 3850.0 22 4465.5 23 5152.0 24 5965.5 25 7146.5 26 8697.0 27 10817.0 28 13011.0 29 15950.0 30 19669.5 31 22863.5 32 26684.0 33 30356.5 34 33706.5 35 37268.5 36 41719.0 37 45976.5 38 49085.0 39 51563.0 40 53506.5 41 56932.0 42 60473.0 43 63454.0 44 66368.5 45 69064.0 46 70636.0 47 73191.0 48 78721.0 49 78385.5 50 72557.5 51 67987.5 52 64860.0 53 63211.5 54 61908.5 55 58255.5 56 52183.5 57 46855.0 58 42788.0 59 37973.0 60 31522.0 61 25742.5 62 20895.5 63 15971.5 64 11826.0 65 8865.5 66 6576.0 67 5097.0 68 3911.5 69 2754.5 70 2049.0 71 1630.5 72 1337.0 73 1132.0 74 929.0 75 745.5 76 514.0 77 311.0 78 212.5 79 126.5 80 71.5 81 41.0 82 25.5 83 12.0 84 6.5 85 5.0 86 2.0 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.7671573837630136E-4 2 0.04994174360188681 3 0.0 4 0.0 5 0.044344824060296044 6 1.0763306810751467E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.007184507296176604 26-27 0.0014530464194514481 28-29 5.3816534053757334E-5 30-31 0.002287202697284687 32-33 3.7671573837630136E-4 34-35 0.012108720162095401 36-37 0.023571641915545713 38-39 0.0040362400540318 40-41 9.417893459407534E-4 42-43 0.0010763306810751467 44-45 0.009337168658326898 46-47 0.0 48-49 0.0 50-51 6.727066756719666E-4 52-53 0.0 54-55 0.0021795696291771723 56-57 0.0 58-59 0.023141109643115654 60-61 0.0 62-63 0.010252049737240771 64-65 0.01557988660856275 66-67 0.006511800620504637 68-69 0.017490373567471136 70-71 1.3454133513439334E-4 72-73 8.072480108063601E-5 74-75 0.02279130217176623 76-77 0.05018391800512872 78-79 0.031832479892797465 80-81 0.027123533163093693 82-83 0.0372141332981732 84-85 0.017275107431256105 86-87 0.010413499339402044 88-89 0.08424978406115712 90-91 0.02607411074904543 92-93 0.025105413136077795 94-95 0.027446432367416243 96-97 0.03896317065492031 98-99 0.05378962578673046 100-101 0.014987904733971418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1858165.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.686928219995465 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0362443792153 42.12875566704176 2 12.78307387050887 13.981340863748365 3 4.1829298988225085 6.862547613785383 4 1.901119478159405 4.158655377589544 5 1.0381021839481661 2.838530980929694 6 0.6328332418261108 2.0764623642582936 7 0.43817384760195993 1.677366722618133 8 0.2974695735973088 1.3014157775158948 9 0.23031459035968968 1.1335677723916209 >10 1.3183025655623761 13.246895763443757 >50 0.09286959910098433 3.53811477299115 >100 0.04462705046973474 4.333419814727154 >500 0.0031517766621665852 1.1815345459325692 >1k 4.92465103463529E-4 0.46914505356822933 >5k 2.9547906207811735E-4 1.0722469094584088 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8044 0.43290019992842405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6791 0.36546808275906606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5072 0.27295746072065724 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2577 0.13868520825653266 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.3816534053757334E-5 0.0 10-11 0.0 0.0 0.0 5.3816534053757334E-5 0.0 12-13 0.0 0.0 0.0 5.3816534053757334E-5 0.0 14-15 0.0 0.0 0.0 1.0763306810751467E-4 0.0 16-17 0.0 0.0 0.0 1.0763306810751467E-4 0.0 18-19 0.0 0.0 0.0 1.61449602161272E-4 0.0 20-21 0.0 0.0 0.0 2.690826702687867E-4 0.0 22-23 0.0 0.0 0.0 5.919818745913307E-4 0.0 24-25 0.0 0.0 0.0 0.0011032389481020254 0.0 26-27 0.0 0.0 0.0 0.0018835786918815068 0.0 28-29 0.0 0.0 0.0 0.0049780293999725535 0.0 30-31 0.0 0.0 0.0 0.01377703271776188 0.0 32-33 0.0 0.0 0.0 0.03390441645386712 0.0 34-35 0.0 0.0 0.0 0.061162490952095215 0.0 36-37 0.0 0.0 0.0 0.10652982915941264 0.0 38-39 0.0 0.0 0.0 0.175872433287679 0.0 40-41 0.0 0.0 0.0 0.24965490147538028 0.0 42-43 0.0 0.0 0.0 0.32615510463279634 0.0 44-45 0.0 0.0 0.0 0.4230786824636133 0.0 46-47 0.0 0.0 0.0 0.5351785228975898 0.0 48-49 0.0 0.0 0.0 0.6580954866763715 0.0 50-51 0.0 0.0 0.0 0.7903227108464533 0.0 52-53 0.0 0.0 0.0 0.9205318149895192 0.0 54-55 0.0 0.0 0.0 1.0640066947768363 0.0 56-57 0.0 0.0 0.0 1.235519988806161 0.0 58-59 0.0 0.0 0.0 1.4212408478256775 0.0 60-61 0.0 0.0 0.0 1.6294301098126378 0.0 62-63 0.0 0.0 0.0 1.831188295980174 0.0 64-65 0.0 0.0 0.0 2.042660366544413 0.0 66-67 0.0 0.0 0.0 2.284861678053348 0.0 68-69 0.0 0.0 0.0 2.5526796597718717 0.0 70-71 0.0 0.0 0.0 2.8477018994545693 0.0 72-73 0.0 0.0 0.0 3.1446346260961757 0.0 74-75 0.0 0.0 0.0 3.446814464808023 0.0 76-77 0.0 0.0 0.0 3.767937723506793 0.0 78-79 0.0 0.0 0.0 4.131360777971816 0.0 80-81 0.0 0.0 0.0 4.5237371277577605 0.0 82-83 0.0 0.0 0.0 4.931424281482 0.0 84-85 0.0 0.0 0.0 5.340053224552179 0.0 86-87 0.0 0.0 0.0 5.758476776820142 0.0 88-89 0.0 0.0 0.0 6.224231970788385 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7800 0.0 31.078749 1 GTATCAA 11540 0.0 29.903275 1 TATCAAC 14000 0.0 24.750696 2 ATCAACG 13995 0.0 24.748873 3 TCAACGC 14215 0.0 24.33242 4 CAACGCA 14445 0.0 23.97788 5 GTACATG 10535 0.0 23.77738 1 TACATGG 10505 0.0 23.573801 2 AACGCAG 14770 0.0 23.500051 6 TAGCCCT 1120 0.0 23.331652 4 GAGTACT 7080 0.0 23.305237 12-13 ACATGGG 10695 0.0 22.21212 3 GTACTTT 7530 0.0 21.660261 14-15 TACCTGG 1475 0.0 21.268646 2 ACGCAGA 16430 0.0 21.010132 7 CGCAGAG 16430 0.0 20.923433 8 AGTACTT 7425 0.0 20.847458 12-13 GTGTAGC 1035 0.0 20.666176 1 GTACCTG 1665 0.0 20.554466 1 CGCGGAA 145 2.4265319E-9 19.66585 82-83 >>END_MODULE