FastQCFastQC Report
Thu 26 May 2016
SRR938394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878920
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85230.4536116492453111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68160.36276158644327594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48860.2600430034275009No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26380.14039980414280545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114650.030.7714421
GGTATCA76450.028.6473241
TTAGACG1004.936568E-528.515383
TATCAAC138400.025.4454462
GTACATG110150.025.372151
TACATGG110050.024.918032
ATCAACG140400.024.8798733
TCAACGC141700.024.618084
CAACGCA142800.024.3932135
AACGCAG146750.023.8617136
ACATGGG112300.023.1914963
GAGTACT72400.021.6164612-13
GGACCGA3600.021.116886
ACGCAGA165000.020.9887477
CGCAGAG165850.020.9098198
CATGGGG47550.020.4894964
AGTACTT74750.020.4603212-13
CATGGGA63900.020.4531294
GTACTTT76350.020.31284714-15
TACCTGG14750.020.2990882
Produced by FastQC (version 0.11.5)