##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938394_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1878920 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.118198752474825 31.0 31.0 34.0 28.0 34.0 2 31.20186756221659 31.0 31.0 34.0 28.0 34.0 3 31.259962638111254 31.0 31.0 34.0 27.0 34.0 4 34.894701743554805 37.0 35.0 37.0 32.0 37.0 5 34.92312392225321 37.0 35.0 37.0 32.0 37.0 6 34.96349126093713 37.0 35.0 37.0 32.0 37.0 7 34.88934334617759 37.0 35.0 37.0 32.0 37.0 8 34.85713015987908 37.0 35.0 37.0 32.0 37.0 9 36.358133395780555 39.0 37.0 39.0 32.0 39.0 10-11 36.20887105358398 39.0 35.0 39.0 32.0 39.0 12-13 36.05959833308496 39.0 35.0 39.0 30.0 39.0 14-15 37.027053573329354 39.0 36.0 41.0 31.0 41.0 16-17 36.90047500691887 39.0 36.0 41.0 31.0 41.0 18-19 36.91491415281119 39.0 36.0 41.0 30.5 41.0 20-21 37.01157233942904 39.0 36.0 41.0 31.0 41.0 22-23 36.92963723841356 39.0 36.0 41.0 30.5 41.0 24-25 36.81761570476657 39.0 36.0 41.0 30.0 41.0 26-27 36.627025365635575 39.0 36.0 41.0 30.0 41.0 28-29 36.44903774508761 39.0 36.0 41.0 30.0 41.0 30-31 36.22995630468567 39.0 35.0 40.0 29.5 41.0 32-33 35.944358195133375 38.0 35.0 40.0 28.5 41.0 34-35 35.68625832925298 38.0 35.0 40.0 27.0 41.0 36-37 35.40808363315096 38.0 34.5 40.0 26.5 41.0 38-39 35.172577065548296 38.0 34.0 40.0 25.5 41.0 40-41 35.105999457134956 38.0 34.0 40.0 25.0 41.0 42-43 34.91456341941115 38.0 34.0 40.0 25.0 41.0 44-45 34.59466289144828 38.0 33.5 40.0 24.0 41.0 46-47 34.20254853852213 38.0 33.0 40.0 23.0 41.0 48-49 33.81835416090094 38.0 33.0 40.0 21.0 41.0 50-51 33.07285009473527 37.5 32.0 39.5 19.0 40.5 52-53 33.1374544951355 37.0 32.0 39.5 19.0 40.5 54-55 33.27319231260512 37.5 32.5 40.0 18.5 41.0 56-57 32.9916523854129 37.0 32.0 40.0 16.5 41.0 58-59 32.6684959977008 37.0 31.5 40.0 13.5 41.0 60-61 32.220031188129354 36.0 31.0 39.0 9.5 41.0 62-63 31.707532252570626 36.0 30.5 39.0 7.5 40.5 64-65 31.2242995976412 35.0 30.0 39.0 6.0 40.0 66-67 30.684502001149596 35.0 29.0 38.0 2.0 40.0 68-69 30.140234017414258 34.5 28.5 38.0 2.0 40.0 70-71 29.533809049879718 34.0 28.0 37.0 2.0 39.5 72-73 28.987145008834865 34.0 27.0 36.5 2.0 39.0 74-75 28.385409703446662 34.0 26.0 36.0 2.0 39.0 76-77 27.663297798735442 33.0 25.5 35.5 2.0 38.0 78-79 26.895910416622314 32.5 24.5 35.0 2.0 37.0 80-81 26.238703084750814 32.0 22.5 35.0 2.0 36.5 82-83 25.59596390479637 31.5 20.5 35.0 2.0 36.0 84-85 24.933341227939458 31.0 18.0 34.5 2.0 36.0 86-87 24.26653742575522 31.0 13.0 34.0 2.0 35.0 88-89 23.493948385242586 30.5 4.5 34.0 2.0 35.0 90-91 22.68360281438273 30.0 2.0 34.0 2.0 35.0 92-93 21.848425957464926 29.0 2.0 34.0 2.0 35.0 94-95 20.898530804930495 29.0 2.0 34.0 2.0 35.0 96-97 19.687841419538884 26.5 2.0 33.5 2.0 35.0 98-99 18.247089019223807 25.0 2.0 33.0 2.0 35.0 100-101 14.822500691886829 8.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4954748384611456 1101 2 0.5837078610335737 1101 3 0.610368509927099 1101 4 0.40886078791776015 1101 5 0.3713154588148484 1101 6 0.5284190390119861 1101 7 0.41120712330348397 1101 8 0.4306793056640643 1101 9 0.4127710285361843 1101 10-11 0.5795894520809952 1101 12-13 0.5037583801694581 1101 14-15 0.6923945023618643 1101 16-17 0.7294858390995103 1101 18-19 0.7665872001570335 1101 20-21 0.686301945964118 1101 22-23 0.7616474539439579 1101 24-25 0.6766574598073376 1101 26-27 0.7206290564927755 1101 28-29 0.7471525272129895 1101 30-31 0.6292882819823689 1101 32-33 0.725352141634886 1101 34-35 0.9575899160811474 1101 36-37 0.8063086069668373 1101 38-39 0.8272074595401122 1101 40-41 0.8516650067614506 1101 42-43 1.038707322018304 1101 44-45 0.9652469968788111 1101 46-47 1.0626586190234804 1101 48-49 0.9602062071840294 1101 50-51 0.9277090142586033 1101 52-53 0.9465042536458839 1101 54-55 0.9908917326930435 1101 56-57 1.0583389852271452 1101 58-59 1.114285741087528 1101 60-61 1.0108098883518437 1101 62-63 0.9480480248330885 1101 64-65 0.9626329250691263 1101 66-67 0.8719137134211294 1101 68-69 0.9187360557060806 1101 70-71 0.9287505687271 1101 72-73 0.9174892419806433 1101 74-75 0.8392675793121889 1101 76-77 1.082289179976069 1101 78-79 1.0120844902450692 1101 80-81 0.9187884082309239 1101 82-83 0.9019284054293664 1101 84-85 1.0036621848520433 1101 86-87 0.9320338095995488 1101 88-89 1.2204710881404175 1101 90-91 1.1695447508384795 1101 92-93 1.01732070419704 1101 94-95 0.8486872493863196 1101 96-97 1.0002627474749701 1101 98-99 1.1447575728785004 1101 100-101 0.9472580558023598 1102 1 0.46082825764259994 1102 2 0.5915545355299088 1102 3 0.580996811982331 1102 4 0.6588486144422561 1102 5 0.6036813100132434 1102 6 0.6066802841150647 1102 7 0.5359246696873257 1102 8 0.5616516996997589 1102 9 0.6269636198749282 1102 10-11 0.6912347146860469 1102 12-13 0.7302573407865296 1102 14-15 0.5633017509129346 1102 16-17 0.6908553434999334 1102 18-19 0.7607442217597296 1102 20-21 0.7156872666076737 1102 22-23 0.8611008359093617 1102 24-25 0.6611197573943244 1102 26-27 0.6749547560908198 1102 28-29 0.4016958131934416 1102 30-31 0.481345628327027 1102 32-33 0.5757776625726905 1102 34-35 0.5342699136696396 1102 36-37 0.47390461221186797 1102 38-39 0.6486083715073292 1102 40-41 0.6326275170944058 1102 42-43 0.7922238622933833 1102 44-45 0.866611121762439 1102 46-47 0.7771352581239839 1102 48-49 0.7243184535844449 1102 50-51 0.8739576128596909 1102 52-53 1.0001213120472698 1102 54-55 0.9489943979885567 1102 56-57 0.8546673200927941 1102 58-59 0.9238528754861761 1102 60-61 0.6904338670842094 1102 62-63 0.83292361191344 1102 64-65 0.7437368795936656 1102 66-67 0.7211823194190572 1102 68-69 0.6912255372435574 1102 70-71 0.6256688534032442 1102 72-73 0.7955710152734277 1102 74-75 0.6173481642418892 1102 76-77 0.7365882718347976 1102 78-79 0.9001244563448374 1102 80-81 0.9721741705977252 1102 82-83 1.0619272924258887 1102 84-85 0.9548701704201008 1102 86-87 0.7235756498348529 1102 88-89 1.1209085796624585 1102 90-91 1.0920027552464369 1102 92-93 0.9833265636965791 1102 94-95 0.7082554851946519 1102 96-97 0.7972029444070188 1102 98-99 0.6338850651077657 1102 100-101 0.48993043511394596 1103 1 0.4445597227015341 1103 2 0.5159883737194626 1103 3 0.5304916153335135 1103 4 0.35830029678567854 1103 5 0.5076145133026984 1103 6 0.44768206455877646 1103 7 0.4829587386877421 1103 8 0.3522404830178232 1103 9 0.3323702566519273 1103 10-11 0.3961938291621081 1103 12-13 0.47507005376742484 1103 14-15 0.8137123934046002 1103 16-17 0.6689250486089762 1103 18-19 0.7307338443487836 1103 20-21 0.5888083821708392 1103 22-23 0.5871387472185248 1103 24-25 0.6220682947410907 1103 26-27 0.7549971338285744 1103 28-29 0.6857587142613681 1103 30-31 0.6079186940377639 1103 32-33 0.865112816066457 1103 34-35 0.8245147218435704 1103 36-37 0.8153198720556603 1103 38-39 0.6385258571894568 1103 40-41 0.7070305464586824 1103 42-43 0.632915426802974 1103 44-45 0.5869166341024297 1103 46-47 0.5347025694497205 1103 48-49 0.6617112893694355 1103 50-51 0.7689598029529847 1103 52-53 0.6644153303691525 1103 54-55 0.7632221461300901 1103 56-57 0.7750019674820976 1103 58-59 0.7066228555483889 1103 60-61 0.7605456469491898 1103 62-63 0.8329970878411324 1103 64-65 0.6484239912918248 1103 66-67 0.9370777157975709 1103 68-69 0.8646178045121005 1103 70-71 0.9602804976640087 1103 72-73 0.9989970644472628 1103 74-75 0.8163465086221464 1103 76-77 0.7068516560849609 1103 78-79 0.6138657881712035 1103 80-81 0.6545533381580171 1103 82-83 0.660584470201325 1103 84-85 0.6682333423560394 1103 86-87 0.9750665870472623 1103 88-89 0.8917524804796031 1103 90-91 0.6900145363596657 1103 92-93 0.7914887179767369 1103 94-95 0.8920855895588424 1103 96-97 0.9166444479230833 1103 98-99 1.1654248198400126 1103 100-101 1.0111607228558892 1104 1 0.34553599196775053 1104 2 0.46321849733524445 1104 3 0.46990986993134953 1104 4 0.45316514699849364 1104 5 0.32759550570297336 1104 6 0.298245322359584 1104 7 0.36116156902777163 1104 8 0.31234416184028646 1104 9 0.39191864360594053 1104 10-11 0.41203732182515296 1104 12-13 0.4165884932412709 1104 14-15 0.5132610735491809 1104 16-17 0.5450181515498471 1104 18-19 0.5594014370550866 1104 20-21 0.5509014696223886 1104 22-23 0.6110731931270834 1104 24-25 0.5703071598612581 1104 26-27 0.4683324088579255 1104 28-29 0.5474398413080266 1104 30-31 0.5664362395569853 1104 32-33 0.4893005952198308 1104 34-35 0.5390968865635628 1104 36-37 0.5041433093775964 1104 38-39 0.5792500544834738 1104 40-41 0.6145056888773794 1104 42-43 0.5668027520088472 1104 44-45 0.6452485568019029 1104 46-47 0.6310185746674009 1104 48-49 0.5127422691016648 1104 50-51 0.543149822524839 1104 52-53 0.6436230022258513 1104 54-55 0.7843751539887052 1104 56-57 0.8784859288517524 1104 58-59 0.7597030713808977 1104 60-61 0.784326859297714 1104 62-63 0.9576337804745272 1104 64-65 0.831148788243528 1104 66-67 0.9115199345752671 1104 68-69 0.8836017553711493 1104 70-71 0.8689887000296714 1104 72-73 0.8759904837020294 1104 74-75 0.604493643818401 1104 76-77 0.7650429302911625 1104 78-79 0.7831787762920079 1104 80-81 0.7882006619919792 1104 82-83 0.7994414584814429 1104 84-85 0.7363211811432677 1104 86-87 0.8602585549534076 1104 88-89 0.8700549956121115 1104 90-91 0.589293066291102 1104 92-93 0.6579856383694143 1104 94-95 0.4385085816736769 1104 96-97 0.40121681565265277 1104 98-99 0.2528253424936828 1104 100-101 0.1711304744405009 1105 1 0.5490378582221247 1105 2 0.6634641874917158 1105 3 0.5376050053775216 1105 4 0.5523853973354065 1105 5 0.5362602032807544 1105 6 0.5706999164396507 1105 7 0.5640178164431049 1105 8 0.4615793406226558 1105 9 0.5923410378106837 1105 10-11 0.6756985714221386 1105 12-13 0.616703178672303 1105 14-15 0.808824821720215 1105 16-17 0.774371793573934 1105 18-19 0.8470413200330285 1105 20-21 0.8811941863689725 1105 22-23 0.7403046523819583 1105 24-25 0.7518473886439878 1105 26-27 0.8686423164976063 1105 28-29 0.9084907190926685 1105 30-31 0.8484415947228499 1105 32-33 0.8864543354246166 1105 34-35 0.9331137987570131 1105 36-37 1.0138416329144988 1105 38-39 0.9113449140911811 1105 40-41 0.8962006860334242 1105 42-43 0.9215242073508065 1105 44-45 0.697272268524749 1105 46-47 0.7284337679834891 1105 48-49 0.7527732598656343 1105 50-51 0.5856300585611791 1105 52-53 0.5569385397571835 1105 54-55 0.6834587302349178 1105 56-57 0.802006323863445 1105 58-59 1.1045814224327657 1105 60-61 1.0180196851398478 1105 62-63 1.0218598737016826 1105 64-65 1.0469839082658403 1105 66-67 1.008683980308497 1105 68-69 0.908988590204256 1105 70-71 0.8879268967953244 1105 72-73 0.7346406237274046 1105 74-75 0.9720326332884817 1105 76-77 0.9329324401904309 1105 78-79 0.9012238360892404 1105 80-81 0.9276442641265206 1105 82-83 0.8614084156191915 1105 84-85 1.0068320306923582 1105 86-87 0.9783234840214341 1105 88-89 0.7908241589277161 1105 90-91 0.8363343331770032 1105 92-93 0.9151219518990885 1105 94-95 0.9513726098954542 1105 96-97 1.0820047938430761 1105 98-99 1.1244762113239126 1105 100-101 0.8447579648217882 1106 1 0.41649053324231033 1106 2 0.3429645463541213 1106 3 0.2578306401154009 1106 4 0.39710451273957403 1106 5 0.49667319409985566 1106 6 0.10658989520695883 1106 7 0.2876619894147865 1106 8 0.37780739768177796 1106 9 0.4588778660651158 1106 10-11 0.36844132688731435 1106 12-13 0.3418239209193743 1106 14-15 0.6378222124914714 1106 16-17 0.33458311119707673 1106 18-19 0.10662313318533734 1106 20-21 0.39939710810706686 1106 22-23 0.45694072852059975 1106 24-25 0.6373880372877849 1106 26-27 0.524659616656102 1106 28-29 0.5231509960148273 1106 30-31 0.6028227065982037 1106 32-33 0.6367038569414021 1106 34-35 0.4228890237936511 1106 36-37 0.38362784811232586 1106 38-39 0.46666953504276165 1106 40-41 0.6520920105347585 1106 42-43 0.4056884691409337 1106 44-45 0.594363800867221 1106 46-47 0.19697910426747 1106 48-49 0.756400199965519 1106 50-51 0.4430056155455375 1106 52-53 0.6704100849588883 1106 54-55 0.5590932812083338 1106 56-57 0.14935242422530592 1106 58-59 -0.08085885688180383 1106 60-61 0.6460531939170266 1106 62-63 0.615388388310361 1106 64-65 1.0298837407967163 1106 66-67 0.3655604942592774 1106 68-69 0.23993449169652692 1106 70-71 0.22699299476476043 1106 72-73 0.7124425541080974 1106 74-75 0.597657838886736 1106 76-77 0.5480263425200498 1106 78-79 0.6582945469358563 1106 80-81 0.5553544229231377 1106 82-83 0.6471815771969851 1106 84-85 0.6518124427860172 1106 86-87 0.6224805685634358 1106 88-89 0.6130872723581398 1106 90-91 0.840289295945027 1106 92-93 0.9417160859940097 1106 94-95 0.5539885673104514 1106 96-97 0.6851076453039475 1106 98-99 0.3928697787152835 1106 100-101 -0.021707838581424 1107 1 0.4517656188863235 1107 2 0.39093434402929006 1107 3 0.33192735197087586 1107 4 0.39514458242598494 1107 5 0.42637922285281604 1107 6 0.34128743275856976 1107 7 0.37492921337754126 1107 8 0.3064447062637541 1107 9 0.14505843585401124 1107 10-11 0.4994144478430087 1107 12-13 0.30315418473222167 1107 14-15 0.36020659307297365 1107 16-17 0.31480904303322177 1107 18-19 0.411337396939615 1107 20-21 0.3987291580001937 1107 22-23 0.42382046822135777 1107 24-25 0.43748887030392325 1107 26-27 0.38894862494234417 1107 28-29 0.4409071228558048 1107 30-31 0.3866557520114924 1107 32-33 0.33676955395191044 1107 34-35 0.43434757062701834 1107 36-37 0.4769002180359081 1107 38-39 0.5880205144589183 1107 40-41 0.5361651519864523 1107 42-43 0.4804760061069331 1107 44-45 0.6556056756659174 1107 46-47 0.45778159599586843 1107 48-49 0.4049532187340077 1107 50-51 0.362735397702302 1107 52-53 0.3875176596961012 1107 54-55 0.370298556001174 1107 56-57 0.4367841652143838 1107 58-59 0.40359529063086086 1107 60-61 0.330438533418679 1107 62-63 0.31742042063549647 1107 64-65 0.3245046379360623 1107 66-67 0.6044221974771489 1107 68-69 0.37899987394699153 1107 70-71 0.37628533365153416 1107 72-73 0.24526452435960877 1107 74-75 0.40344448281259915 1107 76-77 0.5503577628930749 1107 78-79 0.49083305014201883 1107 80-81 0.47686937036552735 1107 82-83 0.42707554698002426 1107 84-85 0.5103021801443788 1107 86-87 0.4784147105128973 1107 88-89 0.523414513010497 1107 90-91 0.523896885322241 1107 92-93 0.5007747704433996 1107 94-95 0.298019106355337 1107 96-97 0.38122237168230555 1107 98-99 0.7894765921723739 1107 100-101 0.634537586417844 1108 1 0.2921385509822656 1108 2 0.2846956580550426 1108 3 0.2611188886802367 1108 4 0.2670057208030343 1108 5 0.3757309302865366 1108 6 0.25851447043694975 1108 7 0.3179114682180284 1108 8 0.1647385226534226 1108 9 0.19695936968032157 1108 10-11 0.21012861311102426 1108 12-13 0.21054253674064682 1108 14-15 0.41496818932336765 1108 16-17 0.07811055910950415 1108 18-19 0.17670336216944094 1108 20-21 0.23096359868699778 1108 22-23 0.2577738674307781 1108 24-25 0.280571463230757 1108 26-27 0.3556861816376724 1108 28-29 0.14537912822542864 1108 30-31 0.3010155344170613 1108 32-33 0.40523349880324133 1108 34-35 0.4288211151732426 1108 36-37 0.3456207514886529 1108 38-39 0.27962518087725385 1108 40-41 0.3320525836585517 1108 42-43 0.39171394784998625 1108 44-45 0.3854055040997153 1108 46-47 0.5857338292853456 1108 48-49 0.5497201323704743 1108 50-51 0.4398277333381486 1108 52-53 0.5281495893189643 1108 54-55 0.511025321217403 1108 56-57 0.5568995299244364 1108 58-59 0.5586134501255628 1108 60-61 0.5234341641349616 1108 62-63 0.593954037697987 1108 64-65 0.38943646407655663 1108 66-67 0.4520972166914987 1108 68-69 0.38140677087819697 1108 70-71 0.48565815848689553 1108 72-73 0.48301684979682236 1108 74-75 0.5186955930102926 1108 76-77 0.5467811424532663 1108 78-79 0.7921209124285618 1108 80-81 0.5849887932275486 1108 82-83 0.7949321636771209 1108 84-85 0.9853890726228727 1108 86-87 0.9342979024761142 1108 88-89 0.7641873774536698 1108 90-91 0.8022847410920129 1108 92-93 0.733302263256558 1108 94-95 0.5557967026889017 1108 96-97 0.7191065033800363 1108 98-99 0.415879759579763 1108 100-101 0.12934825374852998 1109 1 0.4201649487882939 1109 2 0.36986064576649724 1109 3 0.5375882390744948 1109 4 0.5138646740427788 1109 5 0.47963982960816764 1109 6 0.5226331990572888 1109 7 0.4221783029273354 1109 8 0.46768085405147275 1109 9 0.5845307823462207 1109 10-11 0.5290158697284824 1109 12-13 0.2770980165833592 1109 14-15 0.4250879083170318 1109 16-17 0.5677727187896835 1109 18-19 0.5589659694254792 1109 20-21 0.45474497391633406 1109 22-23 0.5087490776288348 1109 24-25 0.3760220389600093 1109 26-27 0.46952899817058125 1109 28-29 0.37371243355798356 1109 30-31 0.45040785580601295 1109 32-33 0.1671562069702901 1109 34-35 0.24447157660235774 1109 36-37 0.4757294106490235 1109 38-39 0.4604339496962737 1109 40-41 0.3441820941925897 1109 42-43 0.352474177555095 1109 44-45 0.44215577539878836 1109 46-47 0.5792783207679264 1109 48-49 0.6457853489384675 1109 50-51 0.7131630846259611 1109 52-53 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-0.7229041911007315 2316 68-69 -0.6956577400547808 2316 70-71 -1.094255331043712 2316 72-73 -0.8477018314096085 2316 74-75 -0.535269863042366 2316 76-77 -0.4181382467089598 2316 78-79 -0.34257036200972735 2316 80-81 -0.38558442048054786 2316 82-83 -0.29564086669059364 2316 84-85 -0.37929079542443134 2316 86-87 -0.43019692274156895 2316 88-89 -0.5075480833265509 2316 90-91 -0.5925172971292199 2316 92-93 -0.6532668750567368 2316 94-95 -0.7189797202616468 2316 96-97 -0.9117131459945753 2316 98-99 -0.904115866442524 2316 100-101 -0.6534019043825516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5906.0 3 2067.0 4 2282.0 5 2547.0 6 2898.0 7 3764.0 8 4732.0 9 5881.0 10 7474.0 11 9341.0 12 11362.0 13 13649.0 14 15612.0 15 18352.0 16 21826.0 17 25394.0 18 23440.0 19 21016.0 20 22162.0 21 24198.0 22 26485.0 23 29432.0 24 32819.0 25 37041.0 26 41975.0 27 47641.0 28 54704.0 29 62141.0 30 72351.0 31 85000.0 32 100053.0 33 121360.0 34 148619.0 35 185668.0 36 224749.0 37 235547.0 38 122208.0 39 7224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01083224785138 18.853741726921356 14.896846779357734 22.238579245869524 2 15.314594793769393 20.338186141886744 40.69841678918051 23.648802275163348 3 19.464171137231933 25.302485323677796 29.27261351685947 25.9607300222308 4 12.813403254217937 16.719561730669692 36.98275549913416 33.484279515978216 5 13.610351655069799 37.88335962287904 33.508786317649424 14.997502404401732 6 31.375723565397955 36.024330011327805 17.784171006683245 14.815775416590999 7 28.70420555523019 32.72123222395553 20.50654073249815 18.068021488316127 8 26.62024022489139 34.70222122838402 19.49937175227873 19.178166794445865 9 27.02248976781853 17.013188026683803 20.02121244229007 35.943109763207595 10-11 25.169038989583715 26.920580560210038 26.852507058931895 21.057873391274345 12-13 26.451631280742998 24.276696776625563 27.367788298352945 21.90388364427849 14-15 23.192031169140932 25.47825975845833 26.27668149593136 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22.326914119165814 26.47695604146981 29.082181024501256 22.11394881486312 42-43 22.13617635159594 26.470146711240766 28.860805314822308 22.53287162234099 44-45 22.205314451274084 26.64754296645156 28.779359177739437 22.36778340453492 46-47 22.472899927342795 26.927797901191525 28.31983541679419 22.279466754671486 48-49 22.32949245286283 26.86578564613421 28.273386943312968 22.53133495768999 50-51 22.374130032742784 27.06116803624993 28.233286821528004 22.33141510947928 52-53 22.555910730192153 27.192762776373314 27.88945626344158 22.36187022999295 54-55 22.18881848258249 27.02565100254245 28.109246906834706 22.676283608040354 56-57 22.29205893820218 27.151706123361443 28.087382684474633 22.46885225396175 58-59 22.5023327263543 26.71454721688314 28.08267192845277 22.700448128309798 60-61 22.341851639827894 26.915873772467773 28.44683756507428 22.295437022630054 62-63 22.247710877745025 26.96074661298791 28.347619251646567 22.443923257620494 64-65 22.009959151563958 26.975722738412728 28.230530060951963 22.783788049071347 66-67 22.068982769343606 27.038481098491268 28.041657806993335 22.850878325171788 68-69 22.149026333221293 26.862030761435072 28.170747707849138 22.818195197494504 70-71 22.206121944344556 26.97275944774154 27.996206556864784 22.82491205104912 72-73 22.334462657897166 26.922719652030697 27.897750214060636 22.8450674760115 74-75 22.14894110275856 27.33487194406065 27.74926412051522 22.76692283266557 76-77 22.270331871413898 26.935033875917558 27.80337622748137 22.99125802518717 78-79 22.423681948462196 26.989884581504832 27.78839321593664 22.798040254096332 80-81 22.320202471816817 27.25896592469581 27.67830719935277 22.7425244041346 82-83 22.449967519238964 27.142259878555752 27.67134632687967 22.736426275325613 84-85 22.39249207612245 27.01306408560715 27.644925125677624 22.949518712592777 86-87 22.445572016114824 26.994965315017776 27.63067479895059 22.92878786991681 88-89 22.45602164007353 27.04476814168903 27.451051897738832 23.048158320498604 90-91 22.419073174887128 27.086155123945822 27.483947738308203 23.010823962858847 92-93 22.65398983493413 26.834496278265686 27.534578924323625 22.976934962476562 94-95 22.498595815930766 26.743623939541557 27.52813026569026 23.229649978837415 96-97 22.540948912687576 26.723874021365575 27.496905372273734 23.23827169367312 98-99 22.56963787744777 26.82330226191183 27.05643773574058 23.550622124899824 100-101 23.10460839892901 26.291195520372252 27.272206500693148 23.33198958000559 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1123.0 1 739.5 2 618.0 3 814.0 4 607.0 5 530.5 6 886.0 7 2008.5 8 3228.0 9 3548.0 10 3081.0 11 2505.0 12 2315.5 13 2380.0 14 2540.0 15 2678.5 16 2738.5 17 2882.0 18 3056.5 19 3233.5 20 3551.0 21 3995.0 22 4536.5 23 5327.5 24 6204.5 25 7267.5 26 8862.5 27 11018.5 28 13159.0 29 15981.0 30 19845.0 31 23164.5 32 26918.0 33 30821.5 34 34234.5 35 37705.5 36 42122.0 37 46422.5 38 49454.0 39 52040.0 40 54068.0 41 57362.0 42 60971.5 43 63763.0 44 66464.5 45 69046.0 46 70807.0 47 73292.0 48 78207.0 49 77838.0 50 72753.0 51 68355.5 52 65177.5 53 63791.5 54 61756.5 55 57821.5 56 52422.5 57 47561.5 58 43725.0 59 38880.0 60 32639.5 61 26943.0 62 21854.0 63 16737.5 64 12558.5 65 9647.5 66 7286.0 67 5554.5 68 4285.5 69 3186.0 70 2466.5 71 1937.5 72 1561.0 73 1316.0 74 1007.5 75 773.5 76 540.0 77 341.5 78 237.5 79 157.5 80 91.0 81 49.5 82 33.5 83 21.5 84 11.5 85 6.5 86 4.5 87 5.0 88 5.5 89 5.0 90 3.5 91 1.5 92 1.5 93 1.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1814340152853767 2 0.04795307942860792 3 0.04763374704617546 4 0.05258339897387861 5 0.05928937900496029 6 0.06594213697230324 7 0.03672322397973304 8 0.0361910033423456 9 0.04135354352500373 10-11 0.042737317182211056 12-13 0.03983671470844954 14-15 0.034700785557660783 16-17 0.03222595959380921 18-19 0.04060843463266131 20-21 0.05072062674302259 22-23 0.04728780363187363 24-25 0.06894918357354225 26-27 0.05311561961126605 28-29 0.04507908798671577 30-31 0.054180060886040916 32-33 0.05034807229685138 34-35 0.049070742767121533 36-37 0.04707491537691865 38-39 0.026344921550678052 40-41 0.023683818363740873 42-43 0.02128882549549741 44-45 0.005295595342004982 46-47 0.012507184978604731 48-49 0.012108019500564154 50-51 0.009872692823536925 52-53 0.010032359014753156 54-55 0.011788687118131693 56-57 0.00918080599493326 58-59 0.011549187831307347 60-61 0.010724245843356822 62-63 0.004710152640878803 64-65 0.007743810273987184 66-67 0.004710152640878803 68-69 0.009127583931194515 70-71 0.022113767483447937 72-73 0.019346220169033274 74-75 0.02051710557128563 76-77 0.012001575373086666 78-79 0.005588316692568071 80-81 0.007451088923424095 82-83 0.006147148361824878 84-85 0.005588316692568071 86-87 0.008142975752027761 88-89 0.029431801247525174 90-91 0.0361910033423456 92-93 0.02275243224831286 94-95 0.03219934856193984 96-97 0.03539267238626445 98-99 0.021208992399889298 100-101 0.12430012986183551 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1878920.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.037988372710124 #Duplication Level Percentage of deduplicated Percentage of total 1 78.62159913115036 44.844178570864315 2 12.051368976051416 13.747716870625204 3 3.9218422068067382 6.710819705743397 4 1.7241635206929962 3.9337127538375225 5 0.9065522723237884 2.5853958984029095 6 0.5832398558998211 1.9960096871589075 7 0.3938440457291726 1.5724850470673162 8 0.2729791056985698 1.2456143245462268 9 0.21736541241635007 1.1158277279429805 >10 1.1792176624929043 12.294463937709905 >50 0.08570357958016886 3.4231273293620816 >100 0.03838073786119256 3.8446566121349695 >500 0.002901207304906026 1.0934960965532896 >1k 6.551113269142639E-4 0.7736945288476141 >5k 1.8717466483264683E-4 0.8188009092033637 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8523 0.4536116492453111 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6816 0.36276158644327594 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4886 0.2600430034275009 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2638 0.14039980414280545 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.322206373874353E-5 0.0 10-11 0.0 0.0 0.0 5.322206373874353E-5 0.0 12-13 0.0 0.0 0.0 5.322206373874353E-5 0.0 14-15 0.0 0.0 0.0 1.0644412747748706E-4 0.0 16-17 0.0 0.0 0.0 1.0644412747748706E-4 0.0 18-19 0.0 0.0 0.0 1.0644412747748706E-4 0.0 20-21 0.0 0.0 0.0 1.596661912162306E-4 0.0 22-23 0.0 0.0 0.0 5.322206373874353E-4 0.0 24-25 0.0 0.0 0.0 0.0011974964341217296 0.0 26-27 0.0 0.0 0.0 0.0024482149319822026 0.0 28-29 0.0 0.0 0.0 0.004976262959572521 0.0 30-31 0.0 0.0 0.0 0.014157068954505782 0.0 32-33 0.0 0.0 0.0 0.03345006705980031 0.0 34-35 0.0 0.0 0.0 0.06099248504460009 0.0 36-37 0.0 0.0 0.0 0.10806740042151874 0.0 38-39 0.0 0.0 0.0 0.17568603240159242 0.0 40-41 0.0 0.0 0.0 0.2505162540182658 0.0 42-43 0.0 0.0 0.0 0.33010985033955675 0.0 44-45 0.0 0.0 0.0 0.4243129031571328 0.0 46-47 0.0 0.0 0.0 0.5425723287846209 0.0 48-49 0.0 0.0 0.0 0.6643976326826049 0.0 50-51 0.0 0.0 0.0 0.7950844101930896 0.0 52-53 0.0 0.0 0.0 0.9265695186596556 0.0 54-55 0.0 0.0 0.0 1.067953930981628 0.0 56-57 0.0 0.0 0.0 1.2345922125476338 0.0 58-59 0.0 0.0 0.0 1.4235305388201733 0.0 60-61 0.0 0.0 0.0 1.6275573201626465 0.0 62-63 0.0 0.0 0.0 1.8340057054052328 0.0 64-65 0.0 0.0 0.0 2.045270687416175 0.0 66-67 0.0 0.0 0.0 2.2887882400527966 0.0 68-69 0.0 0.0 0.0 2.56269559108424 0.0 70-71 0.0 0.0 0.0 2.8557628850616315 0.0 72-73 0.0 0.0 0.0 3.1476859046686396 0.0 74-75 0.0 0.0 0.0 3.4454101292231707 0.0 76-77 0.0 0.0 0.0 3.770570327635024 0.0 78-79 0.0 0.0 0.0 4.139745172758818 0.0 80-81 0.0 0.0 0.0 4.533082834820004 0.0 82-83 0.0 0.0 0.0 4.937064909628935 0.0 84-85 0.0 0.0 0.0 5.339370489430098 0.0 86-87 0.0 0.0 0.0 5.755593638898942 0.0 88-89 0.0 0.0 0.0 6.20848679028378 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11465 0.0 30.771442 1 GGTATCA 7645 0.0 28.647324 1 TTAGACG 100 4.936568E-5 28.51538 3 TATCAAC 13840 0.0 25.445446 2 GTACATG 11015 0.0 25.37215 1 TACATGG 11005 0.0 24.91803 2 ATCAACG 14040 0.0 24.879873 3 TCAACGC 14170 0.0 24.61808 4 CAACGCA 14280 0.0 24.393213 5 AACGCAG 14675 0.0 23.861713 6 ACATGGG 11230 0.0 23.191496 3 GAGTACT 7240 0.0 21.61646 12-13 GGACCGA 360 0.0 21.11688 6 ACGCAGA 16500 0.0 20.988747 7 CGCAGAG 16585 0.0 20.909819 8 CATGGGG 4755 0.0 20.489496 4 AGTACTT 7475 0.0 20.46032 12-13 CATGGGA 6390 0.0 20.453129 4 GTACTTT 7635 0.0 20.312847 14-15 TACCTGG 1475 0.0 20.299088 2 >>END_MODULE