FastQCFastQC Report
Thu 26 May 2016
SRR938394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878920
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83480.444297788091031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71090.37835565111872776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.27622251080407895No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27000.14369957209460754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19120.10176058586847762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83500.028.6505321
GTATCAA117450.027.4554061
GTACATG104250.025.0921481
TACATGG104200.024.6477492
TATCAAC140900.022.8862
ATCAACG141400.022.694493
ACATGGG108150.022.6384543
TCAACGC142200.022.5668144
GAGTACT69800.022.35328312-13
CAACGCA144100.022.2692625
AACGCAG147550.021.7926886
GGACCGA5150.021.2120156
CCGTGCG1353.7990863E-421.1095439
AGGACCG6150.020.8715525
GTACTTT74550.020.54676414-15
AGTACTT74350.020.05903412-13
CATGGGG43100.019.964914
AATACCG1200.00474102519.8086455
CGTCGAT857.9645295E-519.55737144-45
ACGCAGA164200.019.5250427
Produced by FastQC (version 0.11.5)