##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938394_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1878920 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.794536222936582 31.0 31.0 34.0 30.0 34.0 2 32.018226960168604 31.0 31.0 34.0 30.0 34.0 3 32.074669490984185 33.0 31.0 34.0 30.0 34.0 4 35.74468950248015 37.0 35.0 37.0 33.0 37.0 5 35.61947342090137 37.0 35.0 37.0 33.0 37.0 6 35.653848487428945 37.0 35.0 37.0 33.0 37.0 7 35.59736018563856 37.0 35.0 37.0 33.0 37.0 8 35.59720318055053 37.0 35.0 37.0 33.0 37.0 9 37.167424371447424 39.0 37.0 39.0 33.0 39.0 10-11 37.07636142039044 39.0 37.0 39.0 33.0 39.0 12-13 36.971208726289575 39.0 37.0 39.0 33.0 39.0 14-15 37.990749206991254 40.0 37.0 41.0 33.0 41.0 16-17 37.92289001128307 40.0 37.0 41.0 33.0 41.0 18-19 38.05394135993018 40.0 37.0 41.0 33.0 41.0 20-21 38.12151342260448 40.0 37.0 41.0 33.0 41.0 22-23 38.021626519489914 40.0 37.0 41.0 33.0 41.0 24-25 37.91979647882826 40.0 37.0 41.0 33.0 41.0 26-27 37.72890224171333 40.0 37.0 41.0 32.0 41.0 28-29 37.60879148659868 39.5 37.0 41.0 32.0 41.0 30-31 37.37555590445575 39.0 36.0 41.0 31.5 41.0 32-33 37.153692280671876 39.0 36.0 41.0 31.0 41.0 34-35 36.986115161901516 39.0 35.5 41.0 31.0 41.0 36-37 36.98422524641815 39.0 36.0 41.0 31.0 41.0 38-39 36.86363948438465 39.0 35.0 41.0 31.0 41.0 40-41 36.69209785408639 39.0 35.0 41.0 30.0 41.0 42-43 36.474958220679966 39.0 35.0 40.5 30.0 41.0 44-45 36.181789804781474 39.0 35.0 40.0 30.0 41.0 46-47 35.77607162625338 38.0 35.0 40.0 28.5 41.0 48-49 35.401320971622 38.0 34.5 40.0 27.0 41.0 50-51 35.042501011219215 38.0 34.0 40.0 26.0 41.0 52-53 34.56432658122751 38.0 33.0 40.0 24.5 41.0 54-55 34.25982133353203 38.0 33.0 40.0 24.0 41.0 56-57 33.93134513443893 37.0 33.0 40.0 23.0 41.0 58-59 33.49723431545782 37.0 32.0 40.0 22.5 41.0 60-61 33.12867737849403 36.0 32.0 39.0 22.0 40.5 62-63 32.67179363677006 36.0 31.0 39.0 20.5 40.0 64-65 32.172432833755565 35.0 31.0 39.0 19.0 40.0 66-67 31.67107008281353 35.0 30.0 38.0 17.5 40.0 68-69 31.131136770059396 34.5 29.5 38.0 13.5 40.0 70-71 30.55345091861282 34.0 29.0 37.0 10.0 39.0 72-73 30.102938124028697 34.0 29.0 36.5 8.0 39.0 74-75 29.587104293956102 34.0 28.0 36.0 7.0 39.0 76-77 28.310444830008727 31.5 26.5 34.5 6.5 36.5 78-79 29.00982505907649 33.5 28.0 35.0 2.0 37.0 80-81 28.71671279245524 33.0 28.0 35.0 2.0 37.0 82-83 28.239183679986375 33.0 27.0 35.0 2.0 36.0 84-85 27.70768819321738 33.0 26.0 35.0 2.0 36.0 86-87 27.134125721158966 32.5 25.5 35.0 2.0 35.5 88-89 26.494468897025953 32.0 25.0 34.0 2.0 35.0 90-91 25.945559949332598 32.0 23.5 34.0 2.0 35.0 92-93 25.199555329657464 31.0 20.0 34.0 2.0 35.0 94-95 24.42730398313925 31.0 17.5 34.0 2.0 35.0 96-97 23.42389830328061 31.0 4.5 34.0 2.0 35.0 98-99 22.17326229961893 30.0 2.0 34.0 2.0 35.0 100-101 18.49663317224789 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24708990828526467 1101 2 0.2699546847298606 1101 3 0.314700113224724 1101 4 0.21678657329140805 1101 5 0.1059682645186868 1101 6 0.10291708178807824 1101 7 0.19117478666393595 1101 8 0.2291101408038685 1101 9 0.18312101622809251 1101 10-11 0.19731982116586977 1101 12-13 0.31929063289627635 1101 14-15 0.3103376919040599 1101 16-17 0.3555711956732779 1101 18-19 0.437916406857461 1101 20-21 0.35937329765669546 1101 22-23 0.2371935644999894 1101 24-25 0.3721353630259969 1101 26-27 0.3911117244479172 1101 28-29 0.3438730465074329 1101 30-31 0.3070660727911161 1101 32-33 0.26713580028050643 1101 34-35 0.25438166415924 1101 36-37 0.2952888744195974 1101 38-39 0.3319050216026227 1101 40-41 0.39921882226751393 1101 42-43 0.4619828644722901 1101 44-45 0.6427549875752376 1101 46-47 0.6801416747371363 1101 48-49 0.6599317980396577 1101 50-51 0.6638896709976194 1101 52-53 0.803221659564521 1101 54-55 0.7432951038658118 1101 56-57 0.5803665254850401 1101 58-59 0.7579660833385233 1101 60-61 0.6767649202233628 1101 62-63 0.7310299304687433 1101 64-65 0.6969948995980744 1101 66-67 0.7496468217077954 1101 68-69 0.5697256803299311 1101 70-71 0.6315960912760161 1101 72-73 0.5167707903871097 1101 74-75 0.5475628129186703 1101 76-77 0.46908668245101026 1101 78-79 0.4677935040950594 1101 80-81 0.5329605147546168 1101 82-83 0.7133286220945507 1101 84-85 0.7041146229669586 1101 86-87 0.8440655421158887 1101 88-89 0.6640068575290634 1101 90-91 0.7102359991636931 1101 92-93 0.7348926349184914 1101 94-95 0.7710917155912362 1101 96-97 0.7344706976632374 1101 98-99 0.6704605418349416 1101 100-101 0.5009298716925521 1102 1 0.26466724753451487 1102 2 0.3356978290342454 1102 3 0.29198585715740677 1102 4 0.2332355472992944 1102 5 0.19064927888222627 1102 6 0.287059448845028 1102 7 0.31796646139794404 1102 8 0.2849521916982667 1102 9 0.3098457368468317 1102 10-11 0.4129464189345313 1102 12-13 0.3983917028867978 1102 14-15 0.40934848108148714 1102 16-17 0.4625783641112804 1102 18-19 0.4641603911316494 1102 20-21 0.45472871096936984 1102 22-23 0.4724967014307211 1102 24-25 0.4833087585329494 1102 26-27 0.39235037558045605 1102 28-29 0.35755458375073346 1102 30-31 0.29930156925443185 1102 32-33 0.3953774229004452 1102 34-35 0.2812741758486794 1102 36-37 0.26204760541013883 1102 38-39 0.30846470467488984 1102 40-41 0.5149835020297502 1102 42-43 0.4032600475532462 1102 44-45 0.42089615003780523 1102 46-47 0.21351380860826197 1102 48-49 0.4245489538288396 1102 50-51 0.45935397894713503 1102 52-53 0.4033541475562501 1102 54-55 0.41982745458712145 1102 56-57 0.4532971795716847 1102 58-59 0.4300565647241186 1102 60-61 0.5876382685435999 1102 62-63 0.5214334058220871 1102 64-65 0.3807724971568831 1102 66-67 0.44169267281343494 1102 68-69 0.3613860670290805 1102 70-71 0.15361341587072985 1102 72-73 0.1781089046114097 1102 74-75 0.14604666451356962 1102 76-77 0.21495248321643246 1102 78-79 0.21162807351923263 1102 80-81 0.3611340818495954 1102 82-83 0.20546919443340173 1102 84-85 0.10859138959910908 1102 86-87 -4.1990246523582186E-4 1102 88-89 0.13326231037655845 1102 90-91 -0.08411979790330903 1102 92-93 -0.14189164877682003 1102 94-95 -0.013487476345055427 1102 96-97 -0.132587993560616 1102 98-99 -0.14645676316627032 1102 100-101 -0.2444721776250951 1103 1 0.36511082653277427 1103 2 0.29710764768771014 1103 3 0.37438528024371465 1103 4 0.20094245154002266 1103 5 0.1728576078183508 1103 6 0.24074862127346108 1103 7 0.26906440168896495 1103 8 0.2729730622660256 1103 9 0.36535892790353586 1103 10-11 0.3707784705526507 1103 12-13 0.37403868821771624 1103 14-15 0.4572467007512486 1103 16-17 0.5450403410279279 1103 18-19 0.5205341583531009 1103 20-21 0.47991861361802535 1103 22-23 0.5244878707022309 1103 24-25 0.5126110436059221 1103 26-27 0.4699569837614206 1103 28-29 0.5772206569361629 1103 30-31 0.5882822510429477 1103 32-33 0.5892558568362247 1103 34-35 0.46223602396968033 1103 36-37 0.3423169358203211 1103 38-39 0.2840404836048265 1103 40-41 0.4305121428896257 1103 42-43 0.5633272141966472 1103 44-45 0.5571822227466328 1103 46-47 0.3991707401400433 1103 48-49 0.5070350444669245 1103 50-51 0.561625945412608 1103 52-53 0.7292933628219984 1103 54-55 0.6696275943899721 1103 56-57 0.6559470506058247 1103 58-59 0.5774253038091146 1103 60-61 0.6024699093773762 1103 62-63 0.5870454234200011 1103 64-65 0.4678118257854891 1103 66-67 0.3393242514621839 1103 68-69 0.30043778500407115 1103 70-71 0.08324106596871417 1103 72-73 0.08092791212467532 1103 74-75 0.14840285878959847 1103 76-77 0.1826904516041914 1103 78-79 0.08248405956618043 1103 80-81 0.2352784702745545 1103 82-83 0.2821695522242891 1103 84-85 0.19755924856164242 1103 86-87 0.21798842737870316 1103 88-89 0.27140400487329686 1103 90-91 0.17281388993009728 1103 92-93 0.16857322405592257 1103 94-95 -0.020464994413323723 1103 96-97 0.27333260591217723 1103 98-99 -0.05657002957125812 1103 100-101 -0.2522231787700733 1104 1 0.21317062413170973 1104 2 0.1911599174805616 1104 3 0.14160884302908983 1104 4 0.06053625537229834 1104 5 0.08234221199449365 1104 6 0.009712242409293026 1104 7 0.12005444537989263 1104 8 0.1662887599102092 1104 9 0.15837136692819342 1104 10-11 0.30205805928925855 1104 12-13 0.18107932774931612 1104 14-15 0.47035909563137324 1104 16-17 0.3257964463065761 1104 18-19 0.32107675451684514 1104 20-21 0.31306731987316283 1104 22-23 0.25197581393169344 1104 24-25 0.30741042381213646 1104 26-27 0.43867341205862687 1104 28-29 0.43900094324504835 1104 30-31 0.37827302936986484 1104 32-33 0.40725518526387106 1104 34-35 0.355962188406437 1104 36-37 0.3766472000569365 1104 38-39 0.26766897132788614 1104 40-41 0.351821039619054 1104 42-43 0.36622930293050615 1104 44-45 0.3529369616466056 1104 46-47 0.4019864759543239 1104 48-49 0.3878263879324848 1104 50-51 0.5072017550887793 1104 52-53 0.5022371841903919 1104 54-55 0.4541572835305203 1104 56-57 0.4844445374648032 1104 58-59 0.43025096937936524 1104 60-61 0.2545022178713836 1104 62-63 0.22093338479407976 1104 64-65 0.21075768842404585 1104 66-67 0.178339120096215 1104 68-69 0.04027831699558604 1104 70-71 -0.04720882112525615 1104 72-73 -0.1679556126676296 1104 74-75 -0.048402553397618675 1104 76-77 -0.019485326650801937 1104 78-79 0.04502481582082041 1104 80-81 0.09878291072866219 1104 82-83 0.16066087101758342 1104 84-85 0.28079812474560484 1104 86-87 0.19796475638941047 1104 88-89 0.07535673923489838 1104 90-91 0.07243318474593607 1104 92-93 0.14896333899768877 1104 94-95 0.05832512982202687 1104 96-97 -0.06615869739180624 1104 98-99 -0.14059886071451544 1104 100-101 -0.5410584820120938 1105 1 0.29331796404698096 1105 2 0.35108343933265473 1105 3 0.2847936963925477 1105 4 0.26993108242088937 1105 5 0.11201047729853286 1105 6 0.22632809231205187 1105 7 0.17633640675774132 1105 8 0.2008493695312339 1105 9 0.20442701201321256 1105 10-11 0.3541820445987227 1105 12-13 0.40701148134249365 1105 14-15 0.5474566349413976 1105 16-17 0.4214146627719515 1105 18-19 0.5304262796280241 1105 20-21 0.4363268710978616 1105 22-23 0.3744493368037354 1105 24-25 0.48529258737968206 1105 26-27 0.5267112367920532 1105 28-29 0.5345200782800532 1105 30-31 0.5217685056667491 1105 32-33 0.5288735347208942 1105 34-35 0.4562373645607636 1105 36-37 0.42363034270266553 1105 38-39 0.4690778085967082 1105 40-41 0.4543746912863682 1105 42-43 0.47945526978908504 1105 44-45 0.4281013533700744 1105 46-47 0.14277567809183012 1105 48-49 0.08472119180026283 1105 50-51 0.08118405766174419 1105 52-53 0.08638063184798028 1105 54-55 0.21584061345075867 1105 56-57 0.2530142917190119 1105 58-59 0.2878329374067903 1105 60-61 0.2502838392197759 1105 62-63 0.47805817414668894 1105 64-65 0.32357024198829265 1105 66-67 0.18711670162446836 1105 68-69 -0.06129392515624943 1105 70-71 0.11077877144236581 1105 72-73 0.24765866703289063 1105 74-75 0.34467599936708737 1105 76-77 0.2343869955344573 1105 78-79 0.449338646449327 1105 80-81 0.5556415578220602 1105 82-83 0.325716095668831 1105 84-85 0.5447527772413316 1105 86-87 0.6489956138968012 1105 88-89 0.5718786896131185 1105 90-91 0.5612032053543423 1105 92-93 0.41087980169525196 1105 94-95 0.4997899931360159 1105 96-97 0.4533070947443605 1105 98-99 0.3189639941341049 1105 100-101 0.008299435879070671 1106 1 0.10818608211270941 1106 2 0.15295587869985638 1106 3 0.20843450502916738 1106 4 0.11685196821293431 1106 5 0.054250451580060144 1106 6 -0.001330853828683587 1106 7 0.14160338543533157 1106 8 0.13087306940263943 1106 9 0.08333337883040315 1106 10-11 0.08844069467796345 1106 12-13 0.12402697971266008 1106 14-15 0.2479242775144357 1106 16-17 0.05472550490239314 1106 18-19 0.2930780485206341 1106 20-21 0.30365431809089927 1106 22-23 0.3450684118358822 1106 24-25 0.19946428808842853 1106 26-27 0.06170873223151574 1106 28-29 0.12824297669208562 1106 30-31 0.3292388247552438 1106 32-33 0.23552407750028692 1106 34-35 0.10319806165099266 1106 36-37 -0.31715792100138174 1106 38-39 0.0020651138390874735 1106 40-41 0.3206657361740781 1106 42-43 0.4004803079182153 1106 44-45 0.39415801624874547 1106 46-47 0.32088544513032247 1106 48-49 0.47908294005828367 1106 50-51 0.525629540481205 1106 52-53 0.4333837569414314 1106 54-55 0.30596086973832115 1106 56-57 0.4424151410267072 1106 58-59 0.4453567120411961 1106 60-61 0.07673461525972414 1106 62-63 0.06826721149828785 1106 64-65 -0.0895972047056901 1106 66-67 -0.02848951417145784 1106 68-69 -0.492253097081246 1106 70-71 -0.44240669313492376 1106 72-73 -0.07891827157173381 1106 74-75 0.09271720873339362 1106 76-77 0.25225068015421215 1106 78-79 0.1667774903142778 1106 80-81 -0.7702167214326998 1106 82-83 -0.035072392324231316 1106 84-85 -0.11299435185941675 1106 86-87 0.09259814387676712 1106 88-89 0.14720241340868512 1106 90-91 0.16782272148819644 1106 92-93 0.03299208077058324 1106 94-95 0.1238021869815249 1106 96-97 -0.25708720532442086 1106 98-99 -0.31369827896018876 1106 100-101 -0.5826628387104797 1107 1 0.09985226077930065 1107 2 0.12119622361831262 1107 3 0.14588143502067652 1107 4 0.20172555179270546 1107 5 0.03153099794494807 1107 6 0.08146750941905623 1107 7 0.07019128400939678 1107 8 0.1282543389836448 1107 9 0.12613342567841102 1107 10-11 0.10661991758664868 1107 12-13 0.16055470055709975 1107 14-15 0.34198446256407067 1107 16-17 0.419730958303262 1107 18-19 0.26027895327278117 1107 20-21 0.28097878446281044 1107 22-23 0.21639102624750706 1107 24-25 0.21914594123749254 1107 26-27 0.15591806230499827 1107 28-29 0.2715539596218477 1107 30-31 0.24968505202664204 1107 32-33 0.284485735334151 1107 34-35 0.19472644129552918 1107 36-37 0.21041011840949864 1107 38-39 0.07914655617211963 1107 40-41 0.16561804636644695 1107 42-43 0.1694729659570413 1107 44-45 0.19792982985224228 1107 46-47 0.11627128239205575 1107 48-49 0.14366767639184275 1107 50-51 0.1089842238179557 1107 52-53 0.15359684217552427 1107 54-55 0.04300538266544862 1107 56-57 -0.007768591002694336 1107 58-59 -0.040166155642459955 1107 60-61 -0.06390639724283176 1107 62-63 0.06591017112116049 1107 64-65 0.27036659788851836 1107 66-67 0.14240597817181921 1107 68-69 0.13541961834638627 1107 70-71 -0.18048881622806334 1107 72-73 -0.08316844725176153 1107 74-75 -0.0805838189947714 1107 76-77 -0.06095478495866402 1107 78-79 0.09932916952254445 1107 80-81 0.21000829540330201 1107 82-83 0.18209282742220623 1107 84-85 0.31573354173704615 1107 86-87 0.26053727074702593 1107 88-89 0.3171362405980993 1107 90-91 0.2816267796968468 1107 92-93 0.08891139971767714 1107 94-95 -0.019081490879862884 1107 96-97 0.017299378577430247 1107 98-99 0.09558220152468877 1107 100-101 -0.2803376403384483 1108 1 0.1442348113344032 1108 2 0.13789388090022925 1108 3 0.06494432750120893 1108 4 0.07334300833758789 1108 5 0.07325460189021271 1108 6 0.05899553092797305 1108 7 0.12293153819414471 1108 8 0.1744729979119981 1108 9 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-0.18583343252493734 2316 62-63 -0.23431644407190788 2316 64-65 -0.3183504990825661 2316 66-67 -0.19332001578084146 2316 68-69 -0.038027248644187495 2316 70-71 -0.10656184748458486 2316 72-73 -0.1017244055934654 2316 74-75 0.24616496315649883 2316 76-77 0.27359027640402545 2316 78-79 0.4117173814378212 2316 80-81 0.11605652822706247 2316 82-83 0.03067216385299787 2316 84-85 0.030285794389310894 2316 86-87 0.29015220406125053 2316 88-89 0.5162428316325283 2316 90-91 0.44932560541812094 2316 92-93 0.43907614678773754 2316 94-95 0.22328224249987727 2316 96-97 0.3051943396843271 2316 98-99 0.4738180579087512 2316 100-101 0.5216692668823981 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 36.0 5 80.0 6 165.0 7 414.0 8 799.0 9 1380.0 10 2344.0 11 3792.0 12 5175.0 13 7344.0 14 9841.0 15 12862.0 16 16352.0 17 21214.0 18 20021.0 19 16342.0 20 15663.0 21 16932.0 22 18378.0 23 20593.0 24 23931.0 25 28102.0 26 33832.0 27 40324.0 28 48406.0 29 57407.0 30 68147.0 31 81618.0 32 98166.0 33 120819.0 34 153246.0 35 200135.0 36 264588.0 37 306471.0 38 157266.0 39 6725.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.748979726522 19.668711826230982 15.42358646618142 22.1587219810656 2 15.79458834868223 21.01885797809887 39.54041166462991 23.646142008588996 3 19.472831250116425 25.419060569853187 29.70544013667535 25.402668043355035 4 13.283003001724394 17.456198241543014 36.49937197964788 32.76142677708471 5 13.63889878816838 38.03880268969195 33.583061524865485 14.73923699727419 6 31.514433823685945 36.3886700870713 17.425968109339408 14.670927979903347 7 28.631607519213166 32.7803738317757 20.37478977284823 18.2132288761629 8 26.553073041960275 35.14220935430992 19.330466438166606 18.974251165563196 9 26.70502203393439 16.926638707342516 19.962105890618016 36.406233368105084 10-11 25.19442019883763 27.0442062461414 26.79808081238158 20.96329274263939 12-13 26.273976539714305 24.273359163774934 27.498030783641664 21.954633512869094 14-15 23.00576927170928 25.613943116258277 26.19906648500202 25.181221127030422 16-17 21.95748089327912 28.90844208375024 26.797734868967275 22.336342154003365 18-19 22.017861324590722 27.272236178230045 29.36647116428587 21.343431332893363 20-21 23.00238967066187 26.20800779171013 29.324532178059737 21.465070359568262 22-23 22.55665488684989 26.171843399399652 29.25544993932685 22.016051774423605 24-25 22.372539470918056 26.417267412309762 29.388317193699553 21.821875923072625 26-27 22.002051371581956 26.592354568985755 29.54462105780301 21.86097300162928 28-29 21.808512336874376 26.87700913290614 29.543061971771017 21.77141655844847 30-31 22.030341898537458 26.49971792306218 29.403886275094205 22.066053903306155 32-33 22.011075511464032 26.56946543759181 29.177692504204543 22.241766546739616 34-35 22.479583866164372 26.551233617524673 29.102807892064952 21.866374624245996 36-37 22.47173639296659 26.305405557879592 29.200076852741848 22.022781196411977 38-39 22.13865108682838 26.58900698490051 29.23548245365421 22.0368594746169 40-41 22.3016160683919 26.46997927953677 29.149827631081017 22.078577020990313 42-43 22.101593901151304 26.496105192792424 28.917719403569723 22.484581502486545 44-45 22.203000181753396 26.6512748417375 28.799876737667578 22.345848238841526 46-47 22.44374427862815 26.953915015008622 28.32757648010559 22.274764226257638 48-49 22.343314244353138 26.872538479552084 28.274620526685545 22.509526749409233 50-51 22.371468716070932 27.066532901879803 28.2705756498414 22.291422732207863 52-53 22.618046537372532 27.198071232410108 27.830668682008813 22.353213548208544 54-55 22.20858258999851 27.08271240925638 28.047495369680455 22.661209631064654 56-57 22.28317331232836 27.163051114470015 28.0095746492666 22.544200923935026 58-59 22.479429672364976 26.833925872309628 27.969205713920765 22.717438741404635 60-61 22.266488195346263 27.002134204755922 28.34639047963724 22.384987120260575 62-63 22.185590658463372 27.0379526536521 28.246466054967744 22.529990632916782 64-65 21.994337172418195 26.98994635215975 28.116710663572693 22.89900581184936 66-67 21.97771112599555 27.091714147412915 27.917481297096547 23.013093429494983 68-69 22.05618822512546 26.90072750052025 28.07604347013863 22.96704080421566 70-71 22.125035725329298 26.978491355933915 27.872356392159535 23.024116526577252 72-73 22.21627317820876 26.934302684520894 27.793200349136736 23.056223788133607 74-75 21.972303238030356 27.393316373235688 27.5393577161348 23.095022672599153 76-77 21.982521874268198 26.988056968897027 27.84873757264812 23.18068358418666 78-79 22.119382411172374 27.032524003150748 27.808182360079194 23.039911225597685 80-81 21.963000573741787 27.268063020053283 27.724235640754614 23.04470076545031 82-83 22.04892416233259 27.184141001049543 27.695342188122034 23.071592648495827 84-85 21.934495264596084 26.98666614151603 27.77811782464156 23.30072076924633 86-87 21.93427175074413 26.985519240927736 27.710581745168977 23.369627263159153 88-89 21.864713979952796 27.06470833840053 27.53939706176249 23.531180619884186 90-91 21.829484595067495 27.008070042379366 27.69313880073715 23.46930656181599 92-93 21.937256356713064 26.900347783369998 27.654938436036623 23.50745742388031 94-95 21.676467089658903 26.776856117265517 27.715189959314294 23.831486833761282 96-97 21.591233261660957 26.73118067826198 27.73385774806804 23.94372831200903 98-99 21.62525583228419 26.8181156533566 27.39868961712774 24.157938897231464 100-101 21.56563344900262 26.521592191258808 27.70149873331488 24.21127562642369 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1184.0 1 794.5 2 591.5 3 759.5 4 597.5 5 545.5 6 917.5 7 2007.5 8 3117.5 9 3397.0 10 2911.5 11 2303.5 12 2232.0 13 2352.0 14 2484.0 15 2657.0 16 2758.5 17 2838.0 18 3040.0 19 3261.5 20 3481.5 21 3909.0 22 4542.5 23 5224.5 24 6063.5 25 7230.0 26 8782.0 27 10953.0 28 13195.0 29 15961.0 30 19884.5 31 23362.0 32 27009.5 33 30770.0 34 34181.5 35 37786.0 36 42225.0 37 46387.0 38 49576.5 39 52188.5 40 54421.0 41 57765.5 42 61229.5 43 64555.5 44 67452.5 45 69800.5 46 71107.5 47 73700.5 48 79377.0 49 78985.5 50 73268.0 51 68957.5 52 65829.0 53 64045.0 54 62566.5 55 58919.0 56 52783.5 57 47392.5 58 43048.5 59 37935.0 60 31574.0 61 25915.5 62 21165.0 63 15938.5 64 11633.0 65 8824.5 66 6649.0 67 5148.5 68 3930.5 69 2838.5 70 2094.5 71 1625.0 72 1394.0 73 1218.0 74 934.0 75 714.0 76 518.5 77 313.0 78 192.5 79 122.5 80 78.5 81 44.0 82 22.0 83 10.5 84 4.5 85 1.5 86 1.0 87 0.5 88 2.0 89 1.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.386647648649223E-4 2 0.0497626295957252 3 7.98330956081153E-4 4 0.0 5 0.08935984501735039 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.661103186937177E-4 26-27 0.009420305281757605 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010511357588401847 36-37 1.0644412747748708E-4 38-39 0.004337598194707598 40-41 0.005375428437613097 42-43 4.257765099099483E-4 44-45 2.661103186937177E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.661103186937177E-5 68-69 0.0011176633385136141 70-71 5.322206373874354E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0013837736572073318 82-83 6.386647648649223E-4 84-85 0.005002873991441892 86-87 0.002528048027590318 88-89 2.394992868243459E-4 90-91 0.0030602686649777535 92-93 0.0010910523066442425 94-95 2.394992868243459E-4 96-97 0.0 98-99 1.8627722308560236E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1878920.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.73267439392746 #Duplication Level Percentage of deduplicated Percentage of total 1 76.76902328659074 42.01773955084806 2 13.078685237615636 14.316628412221649 3 4.200174286950741 6.896603149362641 4 1.8878439815371697 4.133069997920382 5 1.0205527231758433 2.792878994970971 6 0.6440090632427654 2.114900301912271 7 0.42799632839502844 1.639776857868908 8 0.29953512145571637 1.3115486617744991 9 0.23433468441975783 1.1543187583395316 >10 1.2935722557608298 12.920966421177758 >50 0.09543889054374655 3.623843520454363 >100 0.0448465626242763 4.302107628083437 >500 0.003112255756239051 1.140014376669925 >1k 5.835479542948219E-4 0.5365243230508112 >5k 2.9177397714741095E-4 1.099079045344786 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8348 0.444297788091031 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7109 0.37835565111872776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5190 0.27622251080407895 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2700 0.14369957209460754 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1912 0.10176058586847762 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.6611031869371765E-5 0.0 14-15 0.0 0.0 0.0 1.0644412747748706E-4 0.0 16-17 0.0 0.0 0.0 1.0644412747748706E-4 0.0 18-19 0.0 0.0 0.0 1.8627722308560236E-4 0.0 20-21 0.0 0.0 0.0 2.6611031869371764E-4 0.0 22-23 0.0 0.0 0.0 6.386647648649224E-4 0.0 24-25 0.0 0.0 0.0 0.0013039405615992165 0.0 26-27 0.0 0.0 0.0 0.0025546590594596897 2.6611031869371765E-5 28-29 0.0 0.0 0.0 0.005056096055180636 5.322206373874353E-5 30-31 0.0 0.0 0.0 0.01415706895450578 5.322206373874353E-5 32-33 0.0 0.0 0.0 0.03350328912353905 5.322206373874353E-5 34-35 0.0 0.0 0.0 0.06107231814020821 5.322206373874353E-5 36-37 0.0 0.0 0.0 0.10833351074021247 5.322206373874353E-5 38-39 0.0 0.0 0.0 0.17648436335767356 5.322206373874353E-5 40-41 0.0 0.0 0.0 0.2514742511655632 5.322206373874353E-5 42-43 0.0 0.0 0.0 0.3308815702637685 5.322206373874353E-5 44-45 0.0 0.0 0.0 0.4254837885593852 5.322206373874353E-5 46-47 0.0 0.0 0.0 0.5442754348242607 5.322206373874353E-5 48-49 0.0 0.0 0.0 0.6662870159453302 5.322206373874353E-5 50-51 0.0 0.0 0.0 0.7968407382964682 5.322206373874353E-5 52-53 0.0 0.0 0.0 0.9281927916036872 5.322206373874353E-5 54-55 0.0 0.0 0.0 1.0699497583718305 5.322206373874353E-5 56-57 0.0 0.0 0.0 1.2364815958103592 5.322206373874353E-5 58-59 0.0 0.0 0.0 1.4249143124773807 5.322206373874353E-5 60-61 0.0 0.0 0.0 1.6297394247759351 5.322206373874353E-5 62-63 0.0 0.0 0.0 1.8367998637515168 5.322206373874353E-5 64-65 0.0 0.0 0.0 2.0480116236987205 5.322206373874353E-5 66-67 0.0 0.0 0.0 2.292061396972729 5.322206373874353E-5 68-69 0.0 0.0 0.0 2.566048581099781 5.322206373874353E-5 70-71 0.0 0.0 0.0 2.860206927383816 5.322206373874353E-5 72-73 0.0 0.0 0.0 3.1534072765205545 5.322206373874353E-5 74-75 0.0 0.0 0.0 3.4536329380708066 5.322206373874353E-5 76-77 0.0 0.0 0.0 3.7821195154663316 5.322206373874353E-5 78-79 0.0 0.0 0.0 4.155658569816703 5.322206373874353E-5 80-81 0.0 0.0 0.0 4.555143380239713 5.322206373874353E-5 82-83 0.0 0.0 0.0 4.9668426542907635 5.322206373874353E-5 84-85 0.0 0.0 0.0 5.377716986353863 5.322206373874353E-5 86-87 0.0 0.0 0.0 5.803200774913249 7.98330956081153E-5 88-89 0.0 0.0 0.0 6.268175334766781 1.0644412747748706E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8350 0.0 28.650532 1 GTATCAA 11745 0.0 27.455406 1 GTACATG 10425 0.0 25.092148 1 TACATGG 10420 0.0 24.647749 2 TATCAAC 14090 0.0 22.886 2 ATCAACG 14140 0.0 22.69449 3 ACATGGG 10815 0.0 22.638454 3 TCAACGC 14220 0.0 22.566814 4 GAGTACT 6980 0.0 22.353283 12-13 CAACGCA 14410 0.0 22.269262 5 AACGCAG 14755 0.0 21.792688 6 GGACCGA 515 0.0 21.212015 6 CCGTGCG 135 3.7990863E-4 21.109543 9 AGGACCG 615 0.0 20.871552 5 GTACTTT 7455 0.0 20.546764 14-15 AGTACTT 7435 0.0 20.059034 12-13 CATGGGG 4310 0.0 19.96491 4 AATACCG 120 0.004741025 19.808645 5 CGTCGAT 85 7.9645295E-5 19.557371 44-45 ACGCAGA 16420 0.0 19.525042 7 >>END_MODULE