FastQCFastQC Report
Thu 26 May 2016
SRR938393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885891
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88200.46768344511957477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69370.36783674135991956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50910.2699519749550743No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25070.13293451212185645No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121000.031.232651
GGTATCA80350.029.7148041
TATCAAC144550.025.9042662
ATCAACG145600.025.5216373
TCAACGC146100.025.4342944
GTACATG109100.025.1167891
CAACGCA148200.025.0738895
TACCTGG16000.024.9472392
TACATGG109700.024.6905842
AACGCAG152950.024.386466
ACATGGG110950.023.3416923
GAGTACT71100.022.78293412-13
ATACAGT13050.022.2099676
ACGCAGA169200.021.927987
CGATCCG652.9423254E-421.92407616-17
GTATAGA5450.021.8607221
CGCAGAG169950.021.831218
GGACCGA5300.021.5161196
AGTACTT75000.021.18652712-13
GTACTTT76850.020.9858514-15
Produced by FastQC (version 0.11.5)