##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938393_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1885891 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15640034339206 31.0 31.0 34.0 28.0 34.0 2 31.28831889011613 31.0 31.0 34.0 28.0 34.0 3 31.33508034133468 31.0 31.0 34.0 28.0 34.0 4 35.002363339132536 37.0 35.0 37.0 32.0 37.0 5 35.02231518152428 37.0 35.0 37.0 32.0 37.0 6 35.03602276059433 37.0 35.0 37.0 32.0 37.0 7 34.95564855020783 37.0 35.0 37.0 32.0 37.0 8 34.919458759811675 37.0 35.0 37.0 32.0 37.0 9 36.40375398153976 39.0 37.0 39.0 32.0 39.0 10-11 36.252821345454215 39.0 35.0 39.0 32.0 39.0 12-13 36.082052727331536 39.0 35.0 39.0 30.5 39.0 14-15 37.054382517335306 39.0 36.0 41.0 31.0 41.0 16-17 36.89152130213252 39.0 36.0 41.0 31.0 41.0 18-19 36.78901935477713 39.0 36.0 41.0 30.5 41.0 20-21 36.78825260844874 39.0 36.0 40.5 30.0 41.0 22-23 36.87527831672138 39.0 36.0 41.0 30.0 41.0 24-25 36.78855670873874 39.0 36.0 41.0 30.0 41.0 26-27 36.59027112383483 39.0 36.0 41.0 30.0 41.0 28-29 36.407920977405375 39.0 35.5 41.0 30.0 41.0 30-31 36.134216929822564 39.0 35.0 40.0 29.0 41.0 32-33 35.8309443652894 38.0 35.0 40.0 28.0 41.0 34-35 35.5639610136535 38.0 34.5 40.0 27.0 41.0 36-37 35.26995250520841 38.0 34.0 40.0 26.0 41.0 38-39 34.96315083957663 38.0 34.0 40.0 25.0 41.0 40-41 34.849071340814504 38.0 34.0 40.0 25.0 41.0 42-43 34.605640251743075 38.0 33.0 40.0 24.0 41.0 44-45 34.26985493859401 38.0 33.0 40.0 23.5 41.0 46-47 33.83465004074997 38.0 33.0 40.0 22.0 41.0 48-49 33.42388743570015 38.0 32.5 40.0 19.0 41.0 50-51 32.641268503853084 36.5 31.5 39.5 16.5 40.5 52-53 32.65606045100168 37.0 31.0 39.0 15.5 40.0 54-55 32.7311233788167 37.0 31.0 40.0 14.0 41.0 56-57 32.40736049962591 37.0 31.0 40.0 10.5 41.0 58-59 31.968585936302787 36.0 31.0 39.0 8.0 41.0 60-61 31.472727745134794 36.0 30.0 39.0 6.5 41.0 62-63 30.88882178238297 35.0 29.0 39.0 2.0 40.0 64-65 30.384564378323034 35.0 28.0 38.5 2.0 40.0 66-67 29.8168573899552 34.5 28.0 38.0 2.0 40.0 68-69 29.201567057693154 34.0 26.5 37.5 2.0 40.0 70-71 28.56479483702929 34.0 26.0 37.0 2.0 39.0 72-73 27.915157609851256 33.5 25.5 36.0 2.0 39.0 74-75 27.207182175427953 33.0 24.5 36.0 2.0 38.5 76-77 26.458052188594145 32.5 22.0 35.0 2.0 37.5 78-79 25.66274296870816 32.0 19.5 35.0 2.0 37.0 80-81 24.89277004874619 31.0 16.0 35.0 2.0 36.5 82-83 24.1651219503142 31.0 8.5 34.5 2.0 36.0 84-85 23.41647343351233 30.5 4.0 34.0 2.0 35.5 86-87 22.67434119999512 30.0 2.0 34.0 2.0 35.0 88-89 21.90181670096522 29.0 2.0 34.0 2.0 35.0 90-91 21.082108669058815 29.0 2.0 34.0 2.0 35.0 92-93 20.21494614481961 27.5 2.0 34.0 2.0 35.0 94-95 19.309820928144838 26.0 2.0 34.0 2.0 35.0 96-97 18.24428877384748 24.5 2.0 33.0 2.0 35.0 98-99 17.00805136670147 17.5 2.0 33.0 2.0 35.0 100-101 14.23401299438833 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6127105847332572 1101 2 0.6540687893764954 1101 3 0.5955126412681686 1101 4 0.44482924503282106 1101 5 0.5104287464764496 1101 6 0.44075716282556954 1101 7 0.500746529164644 1101 8 0.3789350142555179 1101 9 0.6044613164675923 1101 10-11 0.6021977283226221 1101 12-13 0.6728426498887643 1101 14-15 0.8356479742281167 1101 16-17 0.9231330394170101 1101 18-19 0.8914678378390803 1101 20-21 0.9480121840643037 1101 22-23 0.894240124401172 1101 24-25 0.9888427881821542 1101 26-27 1.0550881183439884 1101 28-29 1.0793818489402938 1101 30-31 1.0415246092379178 1101 32-33 1.174420449743316 1101 34-35 1.2501893788069296 1101 36-37 1.4013653298246282 1101 38-39 1.2599108847670948 1101 40-41 1.396825092381718 1101 42-43 1.5138617565548813 1101 44-45 1.544862159244623 1101 46-47 1.6434392776800806 1101 48-49 1.5665808552932603 1101 50-51 1.6645140290787737 1101 52-53 1.8888990294200525 1101 54-55 2.0739365216454004 1101 56-57 2.068312744550717 1101 58-59 2.2835531715359387 1101 60-61 2.5278962849031004 1101 62-63 2.4042495529155055 1101 64-65 2.6051737592269184 1101 66-67 2.7127864467314566 1101 68-69 2.9639190988541806 1101 70-71 2.941613672544239 1101 72-73 3.0936400724212696 1101 74-75 3.465281961822363 1101 76-77 3.5126252588008064 1101 78-79 3.791693363795808 1101 80-81 4.226438224034663 1101 82-83 4.0864366615144085 1101 84-85 4.326670953957656 1101 86-87 4.631472859385216 1101 88-89 4.941809132324796 1101 90-91 5.137991280809953 1101 92-93 5.061947872251249 1101 94-95 5.385229954871534 1101 96-97 5.825459477982356 1101 98-99 5.806876577387229 1101 100-101 5.676433172017347 1102 1 0.4554823946951103 1102 2 0.4248758524644707 1102 3 0.3702428256404673 1102 4 0.3460260806697093 1102 5 0.3380386200324281 1102 6 0.3612462787873554 1102 7 0.3175958162893835 1102 8 0.4162562516656436 1102 9 0.5493521468807003 1102 10-11 0.36493016304455494 1102 12-13 0.434094251087771 1102 14-15 0.6773625078537364 1102 16-17 0.7755631877531499 1102 18-19 0.8505444537788165 1102 20-21 0.8385952527111371 1102 22-23 0.6565722226288102 1102 24-25 0.5149812046720186 1102 26-27 0.5837395134373082 1102 28-29 0.4501967600344372 1102 30-31 0.7267525453181136 1102 32-33 0.6528028669174475 1102 34-35 0.7288573517110208 1102 36-37 0.9884055968567083 1102 38-39 0.9424402236710421 1102 40-41 1.1770324208322336 1102 42-43 1.2711636355497902 1102 44-45 1.5400172646518726 1102 46-47 1.4062159492906972 1102 48-49 1.3348440768321836 1102 50-51 1.6720814763722132 1102 52-53 1.654504510444049 1102 54-55 1.788133006326511 1102 56-57 1.7747347062700811 1102 58-59 1.9397142384885449 1102 60-61 1.7888145662896875 1102 62-63 2.113604817164049 1102 64-65 2.1533353858276065 1102 66-67 2.3350667598757155 1102 68-69 2.3991667284329985 1102 70-71 2.3895618451345335 1102 72-73 2.430319977821931 1102 74-75 2.6464656762983765 1102 76-77 2.821279111172462 1102 78-79 3.2463518755904524 1102 80-81 3.7213137993346628 1102 82-83 4.074163202883394 1102 84-85 3.804151879187618 1102 86-87 4.131026375438946 1102 88-89 4.3682093841664305 1102 90-91 4.542384341879195 1102 92-93 4.531281126810587 1102 94-95 4.751579438637332 1102 96-97 4.929825614805733 1102 98-99 5.1950502787479635 1102 100-101 5.117726117806143 1103 1 0.487185863641141 1103 2 0.4527433849051903 1103 3 0.45465751627076756 1103 4 0.3897216902042331 1103 5 0.3176992957904119 1103 6 0.3808586762863868 1103 7 0.47246992462125803 1103 8 0.3529981401955098 1103 9 0.5359766948096905 1103 10-11 0.49706608888254067 1103 12-13 0.724268782327556 1103 14-15 0.8143400238805398 1103 16-17 0.8150168141282848 1103 18-19 0.9156405285464615 1103 20-21 1.0325535021683123 1103 22-23 0.9297426899609817 1103 24-25 1.0298186827781919 1103 26-27 0.9829238070021589 1103 28-29 1.0156021012684775 1103 30-31 1.121894437977268 1103 32-33 1.2240223316929075 1103 34-35 1.3111751382750043 1103 36-37 1.251852451199511 1103 38-39 1.5230026326976684 1103 40-41 1.4822625082010887 1103 42-43 1.7408430479521044 1103 44-45 1.652324865638768 1103 46-47 1.8294339894617835 1103 48-49 1.7358826787929047 1103 50-51 1.9577051881203786 1103 52-53 1.9399664917452597 1103 54-55 1.894019564461722 1103 56-57 1.9616658538336296 1103 58-59 2.2918207775602077 1103 60-61 2.5072629347047695 1103 62-63 2.5696816666549473 1103 64-65 2.7497013543004947 1103 66-67 2.644016217390515 1103 68-69 2.7255594168403654 1103 70-71 3.1104236963631706 1103 72-73 3.1067442380771872 1103 74-75 3.3829925837713546 1103 76-77 3.5381699637671993 1103 78-79 3.79197319579265 1103 80-81 4.11112202831745 1103 82-83 4.401252278507865 1103 84-85 4.714270613423199 1103 86-87 4.717173646495734 1103 88-89 4.881045447991461 1103 90-91 5.060106729846449 1103 92-93 5.27688945696093 1103 94-95 5.291309211147972 1103 96-97 5.481974220998978 1103 98-99 5.39612767881059 1103 100-101 5.437164396994184 1104 1 0.6225504166917766 1104 2 0.4642465674286278 1104 3 0.495444513311071 1104 4 0.4269234894550564 1104 5 0.3608073504818563 1104 6 0.4124063363952928 1104 7 0.4317891394418609 1104 8 0.5323940793985287 1104 9 0.738006983804496 1104 10-11 0.6530602428503229 1104 12-13 0.6219936038204565 1104 14-15 0.7924605174043506 1104 16-17 0.8850947429750988 1104 18-19 1.0026207884698763 1104 20-21 0.8185400242116501 1104 22-23 0.8132208875128484 1104 24-25 0.9532020178087208 1104 26-27 1.004404060903255 1104 28-29 1.0164318129963448 1104 30-31 1.0048303528063087 1104 32-33 1.0049465446027952 1104 34-35 1.1424199577093717 1104 36-37 1.237532464426721 1104 38-39 1.2336103506865612 1104 40-41 1.4674618306351164 1104 42-43 1.2332588914768507 1104 44-45 1.2919856610889582 1104 46-47 1.2600482170332867 1104 48-49 1.4267219879121313 1104 50-51 1.382399610026738 1104 52-53 1.4372074470388227 1104 54-55 1.6776675093717088 1104 56-57 1.8321399419719526 1104 58-59 1.9605067014227338 1104 60-61 2.160991600629046 1104 62-63 2.161566012888091 1104 64-65 2.2691902726805075 1104 66-67 2.403742404308055 1104 68-69 2.652199322497953 1104 70-71 2.7465035370234787 1104 72-73 2.8997314357155624 1104 74-75 2.889191058700426 1104 76-77 3.199563266288145 1104 78-79 3.4280786313215046 1104 80-81 3.8437462606082597 1104 82-83 4.076894258034162 1104 84-85 4.268533787800077 1104 86-87 4.427126026353147 1104 88-89 4.662083400664766 1104 90-91 5.156723663082989 1104 92-93 5.004000527193252 1104 94-95 5.424143841470471 1104 96-97 5.4208835127848545 1104 98-99 5.437553041983982 1104 100-101 5.455627741310046 1105 1 0.6020340832810192 1105 2 0.45277742000996923 1105 3 0.41282513105821295 1105 4 0.47257042847427755 1105 5 0.3703248610162575 1105 6 0.5143848752134659 1105 7 0.45202779240297986 1105 8 0.4373168708481785 1105 9 0.5138409109488151 1105 10-11 0.5305732443581448 1105 12-13 0.49628139152351025 1105 14-15 0.6650559766203585 1105 16-17 0.7742476207602635 1105 18-19 0.8339753205271379 1105 20-21 0.898084449802873 1105 22-23 0.847704311328549 1105 24-25 0.7563526935721754 1105 26-27 0.8191039337424115 1105 28-29 0.9387694729480245 1105 30-31 0.9522440271249337 1105 32-33 0.9715511399780254 1105 34-35 1.1278300547269282 1105 36-37 1.2520274888812395 1105 38-39 1.1297464456018318 1105 40-41 1.2766175429655178 1105 42-43 1.4514915787700815 1105 44-45 1.4327688616842096 1105 46-47 1.4449171258777227 1105 48-49 1.425575309382367 1105 50-51 1.2428828999889063 1105 52-53 1.5695308424040704 1105 54-55 1.7638040898340748 1105 56-57 1.8127071659679572 1105 58-59 1.9185257742377395 1105 60-61 2.103209920977278 1105 62-63 2.4490179010757807 1105 64-65 2.4910884468921815 1105 66-67 2.682478722050078 1105 68-69 2.6104137952134856 1105 70-71 2.892496920460026 1105 72-73 2.9299760333479306 1105 74-75 3.1597867200090164 1105 76-77 3.286726713252623 1105 78-79 3.627366058144961 1105 80-81 3.8371199862111602 1105 82-83 3.9070783591224405 1105 84-85 4.296343432340233 1105 86-87 4.594128639121461 1105 88-89 4.85486029702146 1105 90-91 5.24480308809024 1105 92-93 5.283887722791306 1105 94-95 5.374634440884929 1105 96-97 5.57400433396311 1105 98-99 5.892576682878598 1105 100-101 5.337514472357633 1106 1 0.4082141432808335 1106 2 0.3382809748476667 1106 3 0.25070899890065235 1106 4 0.29842300374060926 1106 5 0.3351355877933173 1106 6 0.043901283017390824 1106 7 0.29643529093194587 1106 8 0.3154924973381341 1106 9 0.20919401868389542 1106 10-11 0.19779186194027432 1106 12-13 0.18421547860617693 1106 14-15 0.6460778427225691 1106 16-17 0.4410636946029527 1106 18-19 0.4326285497625051 1106 20-21 0.48026892751619243 1106 22-23 0.4777805682246381 1106 24-25 0.5788905357113165 1106 26-27 0.6162009588941544 1106 28-29 0.590124339343781 1106 30-31 0.7367335877098711 1106 32-33 0.9151843539172475 1106 34-35 0.7602124475848697 1106 36-37 0.8132637641537599 1106 38-39 0.9664400582146939 1106 40-41 1.0180220230746144 1106 42-43 0.6354244495335379 1106 44-45 0.8656090855185354 1106 46-47 1.1033049237212538 1106 48-49 1.2487109118618633 1106 50-51 0.6600251027114012 1106 52-53 1.1671391428612523 1106 54-55 1.1925397628706307 1106 56-57 1.1173505040533556 1106 58-59 0.4793485799597015 1106 60-61 1.7564837810400107 1106 62-63 2.074991581718269 1106 64-65 2.174097362617321 1106 66-67 2.3650560024182425 1106 68-69 1.7714370829875037 1106 70-71 2.415546037904594 1106 72-73 2.7601798457978255 1106 74-75 2.9538708540200105 1106 76-77 2.9530421743284343 1106 78-79 3.3474029426301755 1106 80-81 3.83360108521612 1106 82-83 3.8425062889181802 1106 84-85 3.881115352742409 1106 86-87 3.9677571536455467 1106 88-89 4.020735961291031 1106 90-91 4.364562088391686 1106 92-93 4.953384306171699 1106 94-95 4.999330196536402 1106 96-97 5.064556271714945 1106 98-99 5.10847650240526 1106 100-101 4.351416579722812 1107 1 0.29221983391715156 1107 2 0.2048283105952109 1107 3 0.3879027392007046 1107 4 0.25916330379234864 1107 5 0.27672689663966565 1107 6 0.1652843663076169 1107 7 0.2717873343877102 1107 8 0.05402043318914451 1107 9 0.1344172468266791 1107 10-11 0.2602736260375238 1107 12-13 0.37726294368636104 1107 14-15 0.44482887738372057 1107 16-17 0.6570141602004682 1107 18-19 0.6032036920284085 1107 20-21 0.6583783429326431 1107 22-23 0.5689104181987759 1107 24-25 0.5365511668546219 1107 26-27 0.4780867581698942 1107 28-29 0.7037395747291981 1107 30-31 0.7201715080409627 1107 32-33 0.7405823104614839 1107 34-35 0.8541061361518629 1107 36-37 0.7598487357005723 1107 38-39 0.8682229087061586 1107 40-41 0.9406531663751849 1107 42-43 0.9649216042153768 1107 44-45 1.0238890906918456 1107 46-47 1.0321792123917248 1107 48-49 1.175040322105012 1107 50-51 1.3356367167828012 1107 52-53 1.3226294429129837 1107 54-55 1.5009898404702113 1107 56-57 1.6787205247465806 1107 58-59 1.8564481660952445 1107 60-61 1.8287608115625176 1107 62-63 1.9360452547653502 1107 64-65 2.062206131370562 1107 66-67 2.088044879810891 1107 68-69 2.2091008181142477 1107 70-71 2.2020611698238923 1107 72-73 2.4768519875464037 1107 74-75 2.7077669999072356 1107 76-77 3.0601083926419363 1107 78-79 3.1265130047098495 1107 80-81 3.261183599442713 1107 82-83 3.4251349748985227 1107 84-85 3.5898396155463423 1107 86-87 3.9706311836507204 1107 88-89 4.304433452237745 1107 90-91 4.6883609761309515 1107 92-93 4.713109732969421 1107 94-95 4.808856451063047 1107 96-97 4.967476344141225 1107 98-99 5.1036282095961525 1107 100-101 4.892740299329645 1108 1 0.27933378283660204 1108 2 0.28208174870916025 1108 3 0.29080745432840516 1108 4 0.27801792068822095 1108 5 0.23240862551982389 1108 6 0.3095957611995601 1108 7 0.16697102790440965 1108 8 0.2872453840732305 1108 9 0.35784651991115624 1108 10-11 0.3866699914599252 1108 12-13 0.48883514407928885 1108 14-15 0.4756545158905894 1108 16-17 0.6182533546994904 1108 18-19 0.6813711772936344 1108 20-21 0.4948390502017972 1108 22-23 0.5059104181987735 1108 24-25 0.4745492022180784 1108 26-27 0.35619432202057055 1108 28-29 0.5760730717822398 1108 30-31 0.5350703292590353 1108 32-33 0.6367409548622689 1108 34-35 0.8888418925369379 1108 36-37 0.9373364567221927 1108 38-39 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2311 26-27 0.3261077133355812 2311 28-29 0.377495422682415 2311 30-31 0.41517053338208854 2311 32-33 0.5193362090969558 2311 34-35 0.3804969743584863 2311 36-37 0.4269077025621755 2311 38-39 0.405900783949825 2311 40-41 0.20448162641417156 2311 42-43 0.4247695574317518 2311 44-45 0.5161985448828759 2311 46-47 0.5172591344190067 2311 48-49 0.4535885677190521 2311 50-51 0.6717142021629172 2311 52-53 0.7005480588973896 2311 54-55 0.6923660587937945 2311 56-57 0.817661557884982 2311 58-59 0.9633809146332517 2311 60-61 1.1261263086385398 2311 62-63 1.255690966266318 2311 64-65 1.2672855660684128 2311 66-67 1.3279815269843809 2311 68-69 1.334258712851092 2311 70-71 1.6599388501357808 2311 72-73 1.7669840538232044 2311 74-75 1.8682811324608402 2311 76-77 2.0151785680805325 2311 78-79 2.3087162210841186 2311 80-81 2.462329798273121 2311 82-83 2.6210487175885824 2311 84-85 2.993632987866029 2311 86-87 3.285961593007446 2311 88-89 3.241914446389792 2311 90-91 3.4900213075149686 2311 92-93 3.885664801195542 2311 94-95 4.0312848136361445 2311 96-97 3.9834812174355747 2311 98-99 4.203095558524026 2311 100-101 3.88620277496317 2312 1 -0.21901522974592424 2312 2 -0.42233329567315536 2312 3 -0.11599882789038318 2312 4 -0.2668964415553532 2312 5 -0.2997197243201768 2312 6 -0.31188458570021993 2312 7 -0.16641393887379508 2312 8 -0.2711573337060571 2312 9 -0.1409407355435519 2312 10-11 -0.3054788225422911 2312 12-13 -0.28416972036848165 2312 14-15 -0.22372915395810367 2312 16-17 -0.039967475450851 2312 18-19 -0.04354434910772653 2312 20-21 -0.09070441906539628 2312 22-23 -0.20356558571895533 2312 24-25 -0.34992875479082386 2312 26-27 -0.0048312144060176365 2312 28-29 -0.05678956337070673 2312 30-31 0.16168619951990593 2312 32-33 -0.005903732633520065 2312 34-35 -0.05688357981287595 2312 36-37 -0.14791693521029003 2312 38-39 -0.028237669158684753 2312 40-41 0.1475562026141688 2312 42-43 0.15569927316738585 2312 44-45 0.19483007991809842 2312 46-47 0.011103796133149046 2312 48-49 0.38622470016573374 2312 50-51 0.40880689308054485 2312 52-53 0.42527709227635313 2312 54-55 0.6036754428208511 2312 56-57 0.6171433944821345 2312 58-59 0.6686034060560679 2312 60-61 0.8321148762050257 2312 62-63 0.9166576445792565 2312 64-65 0.9948190109004287 2312 66-67 1.2257652519950248 2312 68-69 1.4768304949017157 2312 70-71 1.5475814930364287 2312 72-73 1.5397968945003733 2312 74-75 1.5524964318367154 2312 76-77 1.7572357187927672 2312 78-79 1.7101508597539201 2312 80-81 1.877609652332037 2312 82-83 1.9921660718622825 2312 84-85 2.4028484304337034 2312 86-87 2.6018226625929337 2312 88-89 2.5101599458714396 2312 90-91 2.6297955990496575 2312 92-93 2.832896951382743 2312 94-95 2.993477166048354 2312 96-97 2.9998010258258496 2312 98-99 3.1698669461289626 2312 100-101 2.894073551043647 2313 1 -0.25491495300058986 2313 2 -0.4160121254801119 2313 3 -0.3568162895406246 2313 4 -0.3854382819360964 2313 5 -0.2716457495446747 2313 6 -0.293383126259279 2313 7 -0.26547877066679604 2313 8 -0.10384725759381297 2313 9 -0.29102824375806335 2313 10-11 -0.2986106653400711 2313 12-13 -0.37839587692810284 2313 14-15 -0.1968309343110306 2313 16-17 -0.23964515595413616 2313 18-19 -0.0018138996465140167 2313 20-21 0.03450173242719501 2313 22-23 -0.16021941331758427 2313 24-25 -0.11774417506807566 2313 26-27 -0.039671666012466744 2313 28-29 0.11047173528420018 2313 30-31 0.26591828703006115 2313 32-33 0.17308454039210375 2313 34-35 0.1911284354581042 2313 36-37 0.17037029169661366 2313 38-39 0.2603921356635368 2313 40-41 0.3216164964049142 2313 42-43 0.19989286288733155 2313 44-45 0.2178353245867939 2313 46-47 0.28716236402699735 2313 48-49 0.37143105550441646 2313 50-51 0.605652326297168 2313 52-53 0.6108794429129816 2313 54-55 0.5170631804107444 2313 56-57 0.6944913374324102 2313 58-59 0.7056102077206141 2313 60-61 1.0206443596299124 2313 62-63 1.0765826680458233 2313 64-65 1.0719531581297268 2313 66-67 1.0025765943797715 2313 68-69 1.4853723741102876 2313 70-71 1.6148572550567977 2313 72-73 1.7015157635622629 2313 74-75 1.5398718066465804 2313 76-77 1.8198140417995177 2313 78-79 1.8256044318654467 2313 80-81 1.9845820137142702 2313 82-83 2.1010257082979287 2313 84-85 2.20795458085853 2313 86-87 2.2727109656130615 2313 88-89 2.156322203476595 2313 90-91 2.2682299057642545 2313 92-93 2.545250218598756 2313 94-95 2.699872060577416 2313 96-97 3.092849485865706 2313 98-99 3.15805115310458 2313 100-101 3.174679100122507 2314 1 -0.4898078340670402 2314 2 -0.4976459977051846 2314 3 -0.5943719643566077 2314 4 -0.38580112308512327 2314 5 -0.5135339729255506 2314 6 -0.32324725424574297 2314 7 -0.3386889501972661 2314 8 -0.29846770910334897 2314 9 -0.3807665476397304 2314 10-11 -0.48673131467393205 2314 12-13 -0.364696542479642 2314 14-15 -0.41600412656885055 2314 16-17 -0.39055402161771724 2314 18-19 -0.36405618939451045 2314 20-21 -0.2329971511385054 2314 22-23 -0.4765500561095237 2314 24-25 -0.2993302560702844 2314 26-27 -0.31800118649414344 2314 28-29 -0.2744155636107237 2314 30-31 -0.3807395591527083 2314 32-33 -0.21821907293772824 2314 34-35 -0.282431413255253 2314 36-37 -0.3180119362361893 2314 38-39 -0.1515251150092638 2314 40-41 -0.005635371174221859 2314 42-43 -0.05019894914430978 2314 44-45 -0.05507434804332689 2314 46-47 0.054119923854173635 2314 48-49 0.22193854344888564 2314 50-51 0.11726813970770422 2314 52-53 0.037756913268715664 2314 54-55 0.09103331873108544 2314 56-57 0.1263163745489564 2314 58-59 0.40872682222172685 2314 60-61 0.37329045583128817 2314 62-63 0.6533407093108003 2314 64-65 0.5776339969832058 2314 66-67 0.6978274885065439 2314 68-69 0.850498051315828 2314 70-71 0.8663546480847586 2314 72-73 0.9643332128428455 2314 74-75 1.024096051290634 2314 76-77 1.130487839282253 2314 78-79 1.531662214196011 2314 80-81 1.8048713464782864 2314 82-83 1.8702584926850854 2314 84-85 2.254517481158988 2314 86-87 2.1735936342436055 2314 88-89 2.3950866142930813 2314 90-91 2.503199908937276 2314 92-93 2.6401581519417547 2314 94-95 2.7853515103515853 2314 96-97 2.8392668579752183 2314 98-99 2.8279039012957554 2314 100-101 2.832597018697232 2315 1 -0.5814554520102497 2315 2 -0.41695786543661484 2315 3 -0.44046548556309517 2315 4 -0.32857264847021384 2315 5 -0.34407469460846585 2315 6 -0.24256968640247578 2315 7 -0.4395843712313834 2315 8 -0.517705822405091 2315 9 -0.41796241503309517 2315 10-11 -0.20153679166510585 2315 12-13 -0.41868198421020253 2315 14-15 -0.4188808441478571 2315 16-17 -0.30125821672643127 2315 18-19 -0.36632999767819996 2315 20-21 -0.3423071369281203 2315 22-23 -0.3437290646455793 2315 24-25 -0.40531158799371525 2315 26-27 -0.41292107375453213 2315 28-29 -0.43178889369446694 2315 30-31 -0.4077640961360629 2315 32-33 -0.3435747009756156 2315 34-35 -0.31646348095405585 2315 36-37 -0.34335427275293284 2315 38-39 -0.22820859303426744 2315 40-41 -0.246259812242414 2315 42-43 0.008926576865796676 2315 44-45 -0.10844643889542027 2315 46-47 0.10008672109385941 2315 48-49 0.2457895762074429 2315 50-51 0.20572087889119928 2315 52-53 0.4471549277463964 2315 54-55 0.4914532914896057 2315 56-57 0.7616555073423044 2315 58-59 0.9100063461051882 2315 60-61 0.9372041233476978 2315 62-63 0.9963151453670385 2315 64-65 1.0380988464376877 2315 66-67 1.1734439350073131 2315 68-69 1.2644126032957494 2315 70-71 1.2650963513256315 2315 72-73 1.497463250599612 2315 74-75 1.6739685165656155 2315 76-77 1.6654027735469583 2315 78-79 1.9307406277829458 2315 80-81 2.0167087113273467 2315 82-83 2.2168064070218456 2315 84-85 2.235494513607005 2315 86-87 2.5735807112489333 2315 88-89 2.9520859882894612 2315 90-91 3.1795878533064865 2315 92-93 3.1591938950778236 2315 94-95 2.9780858145637943 2315 96-97 2.907639695707612 2315 98-99 3.1634403677264373 2315 100-101 3.04030307904123 2316 1 -0.9538792276052668 2316 2 -0.7162644944100052 2316 3 -0.7411342785260935 2316 4 -0.620454005905259 2316 5 -0.547264761337388 2316 6 -0.4288975940677773 2316 7 -0.535226742776004 2316 8 -0.6643982320871871 2316 9 -0.6838825446227474 2316 10-11 -0.5048481153597564 2316 12-13 -0.5646789228412672 2316 14-15 -1.0677334271001158 2316 16-17 -0.8623463716034934 2316 18-19 -0.6671041807557359 2316 20-21 -0.5025356299557799 2316 22-23 -0.7304034504143644 2316 24-25 -0.8470609290786442 2316 26-27 -0.6621173607039381 2316 28-29 -0.7147638142176262 2316 30-31 -0.5782294304366147 2316 32-33 -0.29929542676479315 2316 34-35 -0.3994595572787958 2316 36-37 -0.3033381777509305 2316 38-39 -0.2605453394690258 2316 40-41 -0.3401163852410818 2316 42-43 -0.15928486085240223 2316 44-45 -0.3059813472643569 2316 46-47 -0.010127356951343813 2316 48-49 0.026912845566954502 2316 50-51 0.13658379707477053 2316 52-53 0.19482104875239514 2316 54-55 0.3879741991772008 2316 56-57 0.5681107750072698 2316 58-59 0.6935487917469665 2316 60-61 0.35783849665114786 2316 62-63 0.4955236176433466 2316 64-65 0.8929644733934978 2316 66-67 0.8405344522822169 2316 68-69 0.9790778775511626 2316 70-71 0.9230713366643499 2316 72-73 1.1651713306120968 2316 74-75 1.2793585014713642 2316 76-77 1.5274629286169095 2316 78-79 1.7698088858360208 2316 80-81 1.9096288549171625 2316 82-83 2.103222826827615 2316 84-85 2.1553536927100545 2316 86-87 2.395264656017769 2316 88-89 2.218373233259644 2316 90-91 2.4538461169025894 2316 92-93 2.6721736015825606 2316 94-95 2.6854437816157066 2316 96-97 2.7887239979472724 2316 98-99 2.7284199197108556 2316 100-101 2.7617848770147333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5530.0 3 2071.0 4 2216.0 5 2516.0 6 3093.0 7 3806.0 8 4924.0 9 6400.0 10 8012.0 11 10054.0 12 12384.0 13 15116.0 14 17364.0 15 20665.0 16 23946.0 17 28150.0 18 27417.0 19 24899.0 20 26412.0 21 28654.0 22 31979.0 23 35434.0 24 39495.0 25 44237.0 26 49861.0 27 55346.0 28 62059.0 29 69479.0 30 78682.0 31 89006.0 32 101425.0 33 118478.0 34 140053.0 35 168255.0 36 195254.0 37 205703.0 38 118339.0 39 9176.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99582166331414 18.88596480963996 14.826553287089386 22.291660239956514 2 15.317173718861325 20.295649019029575 40.691548611449996 23.69562865065911 3 19.415906454215644 25.426646416732403 29.23385332094855 25.923593808103405 4 12.815703116794106 16.687479244890664 37.046742893397514 33.45007474491772 5 13.66443018538778 37.85556709815228 33.43925051172375 15.04075220473619 6 31.37146398219881 36.06326976928276 17.776676580974925 14.788589667543498 7 28.714424148431107 32.758133271241284 20.47000779156167 18.057434788765935 8 26.603515282094044 34.802268697313075 19.448833640709676 19.145382379883213 9 27.057522358607667 17.020496716493916 20.009972522517742 35.91200840238067 10-11 25.14320494239049 26.90112699551403 26.870203904106592 21.085464157988884 12-13 26.424833226319173 24.298022903417532 27.384062024188115 21.89308184607518 14-15 23.165835582427707 25.47973534367055 26.284980522581954 25.069448551319795 16-17 22.055462772279434 28.799475157211525 26.77029695303728 22.37476511747176 18-19 22.092319262850353 27.166561164342568 29.353389647795495 21.38772992501158 20-21 23.164393694860465 26.134724337795113 29.307425017657646 21.393456949686776 22-23 22.68575268831463 26.09223307408042 29.186400599589774 22.03561363801518 24-25 22.379076326271626 26.336033376695305 29.427537046900966 21.857353250132103 26-27 22.051168880221873 26.42752030182722 29.5297954645283 21.99151535342261 28-29 21.877159936197177 26.860920133483063 29.478066585578166 21.7838533447416 30-31 22.08992719795266 26.44327691698487 29.410711600295038 22.056084284767426 32-33 21.956777322880086 26.498401964479086 29.21006310191544 22.33475761072539 34-35 22.521679100252033 26.553115221132522 29.041998192844737 21.883207485770708 36-37 22.507166633206026 26.21774418335124 29.157436258801322 22.11765292464142 38-39 22.159490780148612 26.549056482693917 29.218037592194534 22.073415144962933 40-41 22.3334918184805 26.451816316686738 29.10794959907147 22.106742265761294 42-43 22.143494699416138 26.445694538507926 28.91723197565481 22.49357878642113 44-45 22.186710023720167 26.61158475439576 28.80560756881792 22.39609765306615 46-47 22.551045545028085 26.9029598341744 28.256696624488125 22.289297996309386 48-49 22.385765844741304 26.799057186881758 28.31406527302363 22.501111695353313 50-51 22.426328854017356 27.019072754548652 28.237349127336763 22.317249264097224 52-53 22.590736631111746 27.149932522543303 27.938663124431613 22.32066772191334 54-55 22.19012814621889 27.052133194009574 28.130336829596096 22.627401830175437 56-57 22.313002835925687 27.115304665808328 28.087303876754955 22.484388621511037 58-59 22.51451398051086 26.723727415037267 28.071268575986018 22.690490028465856 60-61 22.32487044502746 26.914026405484197 28.420615845146514 22.34048730434183 62-63 22.288545051872948 26.959456933481352 28.314127169260733 22.437870845384968 64-65 22.04658081245973 26.978239484980815 28.240094816134654 22.7350848864248 66-67 22.11472841087152 27.011286717625254 28.004926173678115 22.86905869782511 68-69 22.16152481272862 26.81642367080539 28.21307097487175 22.808980541594238 70-71 22.26040509911108 26.99362345955956 27.94763854815974 22.798332893169615 72-73 22.413364275736694 26.895310592505307 27.885630131508748 22.80569500024925 74-75 22.141889905040994 27.28255719269442 27.721251720507173 22.854301181757414 76-77 22.31502494669809 26.94930134131755 27.79391219430451 22.941761517679847 78-79 22.46054626072067 27.08655204239933 27.71308174396385 22.739819952916147 80-81 22.405788009665066 27.257199416362788 27.612493739263282 22.724518834708864 82-83 22.53557950045912 27.158388934658024 27.57959167331107 22.726439891571783 84-85 22.507373442456394 26.998438877052024 27.59401470552364 22.900172974967944 86-87 22.479112408951103 27.07890290335726 27.55147031725701 22.89051437043463 88-89 22.53736927812968 26.991373026128596 27.443763442770624 23.0274942529711 90-91 22.49464624083231 27.07141748978642 27.47547878850563 22.958457480875644 92-93 22.784085470316633 26.868569395180096 27.433755970388095 22.913589164115173 94-95 22.62117382623608 26.7111061963682 27.54381038073258 23.123909596663136 96-97 22.70650728721342 26.671503418342425 27.466677061440002 23.155312233004146 98-99 22.736944986767263 26.782362397976943 27.064729701386437 23.415962913869354 100-101 23.217204798798626 26.29571995382851 27.32643025851629 23.16064498885658 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1101.0 1 739.5 2 629.5 3 819.5 4 635.0 5 559.5 6 927.5 7 2110.0 8 3335.5 9 3685.0 10 3129.0 11 2498.5 12 2431.0 13 2514.0 14 2614.0 15 2704.5 16 2832.5 17 2956.5 18 3135.5 19 3339.5 20 3615.5 21 4061.5 22 4637.0 23 5380.5 24 6286.5 25 7553.5 26 9049.0 27 11157.0 28 13626.5 29 16555.5 30 20039.0 31 23250.0 32 26959.0 33 30783.0 34 34412.5 35 38057.5 36 42446.0 37 46579.5 38 49166.5 39 51554.5 40 54049.5 41 57301.5 42 60469.0 43 63249.5 44 66029.5 45 69045.5 46 71001.0 47 73238.5 48 77826.0 49 77626.0 50 72519.0 51 67862.0 52 64901.0 53 63374.5 54 61662.5 55 58082.5 56 52759.0 57 48003.5 58 43956.0 59 38944.5 60 32847.5 61 27394.5 62 22496.5 63 17417.5 64 13146.5 65 10173.5 66 7796.5 67 5963.5 68 4627.0 69 3460.5 70 2597.0 71 2036.0 72 1667.5 73 1383.0 74 1059.0 75 811.0 76 611.0 77 418.0 78 265.5 79 165.0 80 116.0 81 77.5 82 45.5 83 29.0 84 13.5 85 8.5 86 10.5 87 8.5 88 8.0 89 6.0 90 2.0 91 1.5 92 1.5 93 2.5 94 2.0 95 0.5 96 1.0 97 0.5 98 0.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30314583398510303 2 0.03261058035697715 3 0.04146581112057908 4 0.04310959647190638 5 0.05753248729645563 6 0.056896183289490226 7 0.027467122967340104 8 0.030754693669994712 9 0.037701012412700415 10-11 0.030144902329986196 12-13 0.03322037169698567 14-15 0.026645230291676456 16-17 0.01983147488375521 18-19 0.02473631827078023 20-21 0.038735006424019204 22-23 0.03192125101609796 24-25 0.05212390323724966 26-27 0.044488255153664766 28-29 0.03754193641095906 30-31 0.03767449974574352 32-33 0.03221289035262377 34-35 0.03221289035262377 36-37 0.031364485010003225 38-39 0.018028613530686556 40-41 0.017524872858505607 42-43 0.01824071486634169 44-45 0.008165901422722735 46-47 0.01566898617152317 48-49 0.013654023482799376 50-51 0.0059388373983438065 52-53 0.004029925377447584 54-55 0.006124426067042051 56-57 0.004613204050499207 58-59 0.00652211607139543 60-61 0.005753248729645563 62-63 0.004321564713973395 64-65 0.007211445412274623 66-67 0.0024391653600340634 68-69 0.006628166739222998 70-71 0.012142801466256533 72-73 0.012567004137566807 74-75 0.009677123439265578 76-77 0.00904081943230017 78-79 0.005647198061817995 80-81 0.006919806075748811 82-83 0.011639060794075586 84-85 0.003658748040051095 86-87 0.0011930700130601396 88-89 0.018638404870695072 90-91 0.02828901564300376 92-93 0.012222339467127209 94-95 0.021607823569866975 96-97 0.017392309523721148 98-99 0.012010238131472074 100-101 0.10841029518673137 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1885891.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.87892027321714 #Duplication Level Percentage of deduplicated Percentage of total 1 78.37693434326768 44.57995399769897 2 12.295312779385052 13.986882306258206 3 3.84614874891503 6.562943641454155 4 1.7650077948074012 4.015669505698279 5 0.9177369755490785 2.6099944132019735 6 0.5975194808189014 2.0391757746715435 7 0.3839409010228 1.5286700729232097 8 0.2852628736894713 1.2980355399593797 9 0.21914563687678662 1.1218290487324325 >10 1.183063774608983 12.239362089876183 >50 0.0874202660218533 3.4740314328329855 >100 0.03894167658999486 3.880212150736689 >500 0.0027204659200249404 1.0424482677278748 >1k 5.628550179361943E-4 0.5083916207662993 >5k 2.8142750896809716E-4 1.1124001374617767 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8820 0.46768344511957477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6937 0.36783674135991956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5091 0.2699519749550743 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2507 0.13293451212185645 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.302533391378399E-5 0.0 12-13 0.0 0.0 0.0 5.302533391378399E-5 0.0 14-15 0.0 0.0 0.0 7.953800087067598E-5 0.0 16-17 0.0 0.0 0.0 1.0605066782756797E-4 0.0 18-19 0.0 0.0 0.0 1.0605066782756797E-4 0.0 20-21 0.0 0.0 0.0 1.3256333478445996E-4 0.0 22-23 0.0 0.0 0.0 7.423546747929758E-4 0.0 24-25 0.0 0.0 0.0 0.0015642473504566276 0.0 26-27 0.0 0.0 0.0 0.0024126526930771715 0.0 28-29 0.0 0.0 0.0 0.005673710728774887 0.0 30-31 0.0 0.0 0.0 0.014926631496730191 0.0 32-33 0.0 0.0 0.0 0.034148315040476884 0.0 34-35 0.0 0.0 0.0 0.06050190599562753 0.0 36-37 0.0 0.0 0.0 0.10554692715538702 0.0 38-39 0.0 0.0 0.0 0.17307468989459093 0.0 40-41 0.0 0.0 0.0 0.24550729602081986 0.0 42-43 0.0 0.0 0.0 0.32509832222540963 0.0 44-45 0.0 0.0 0.0 0.41529441521275623 0.0 46-47 0.0 0.0 0.0 0.5269922811021421 0.0 48-49 0.0 0.0 0.0 0.6463788204090268 0.0 50-51 5.302533391378399E-5 0.0 0.0 0.7767681165030217 0.0 52-53 5.302533391378399E-5 0.0 0.0 0.9054075765778615 0.0 54-55 5.302533391378399E-5 0.0 0.0 1.0465345027893977 0.0 56-57 5.302533391378399E-5 0.0 0.0 1.2150225013004463 0.0 58-59 5.302533391378399E-5 0.0 0.0 1.403633614031776 0.0 60-61 5.302533391378399E-5 0.0 0.0 1.6122087649816452 0.0 62-63 5.302533391378399E-5 0.0 0.0 1.8141292365253348 0.0 64-65 5.302533391378399E-5 0.0 0.0 2.0223332101378073 0.0 66-67 5.302533391378399E-5 0.0 0.0 2.2606290607463526 0.0 68-69 5.302533391378399E-5 0.0 0.0 2.527691155003126 5.302533391378399E-5 70-71 5.302533391378399E-5 0.0 0.0 2.823652056242911 5.302533391378399E-5 72-73 5.302533391378399E-5 0.0 0.0 3.119719008150524 5.302533391378399E-5 74-75 5.302533391378399E-5 0.0 0.0 3.4207703414460324 5.302533391378399E-5 76-77 5.302533391378399E-5 0.0 0.0 3.743721137647934 5.302533391378399E-5 78-79 5.302533391378399E-5 0.0 0.0 4.106149294948647 1.0605066782756797E-4 80-81 5.302533391378399E-5 0.0 0.0 4.48949594647835 1.0605066782756797E-4 82-83 5.302533391378399E-5 0.0 0.0 4.880133581421196 1.0605066782756797E-4 84-85 5.302533391378399E-5 0.0 0.0 5.272229413046672 1.0605066782756797E-4 86-87 5.302533391378399E-5 0.0 0.0 5.668699834720034 1.0605066782756797E-4 88-89 5.302533391378399E-5 0.0 0.0 6.109791074881846 1.0605066782756797E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12100 0.0 31.23265 1 GGTATCA 8035 0.0 29.714804 1 TATCAAC 14455 0.0 25.904266 2 ATCAACG 14560 0.0 25.521637 3 TCAACGC 14610 0.0 25.434294 4 GTACATG 10910 0.0 25.116789 1 CAACGCA 14820 0.0 25.073889 5 TACCTGG 1600 0.0 24.947239 2 TACATGG 10970 0.0 24.690584 2 AACGCAG 15295 0.0 24.38646 6 ACATGGG 11095 0.0 23.341692 3 GAGTACT 7110 0.0 22.782934 12-13 ATACAGT 1305 0.0 22.209967 6 ACGCAGA 16920 0.0 21.92798 7 CGATCCG 65 2.9423254E-4 21.924076 16-17 GTATAGA 545 0.0 21.860722 1 CGCAGAG 16995 0.0 21.83121 8 GGACCGA 530 0.0 21.516119 6 AGTACTT 7500 0.0 21.186527 12-13 GTACTTT 7685 0.0 20.98585 14-15 >>END_MODULE