FastQCFastQC Report
Thu 26 May 2016
SRR938393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885891
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83970.4452537288740442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69340.36767766535817814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52430.27801182570996946No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26630.14120646421240676No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19100.10127838777532741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81700.029.6305521
GTATCAA116400.027.9477711
TACATGG105900.024.4762332
GTACATG107200.024.223781
TATCAAC139250.023.2592622
ATCAACG138800.023.2232443
TCAACGC141400.022.863474
ACATGGG109600.022.6434523
TACCTGG16400.022.6201152
CAACGCA142950.022.5823065
TAAGGTG7000.022.4129245
AACGCAG146600.022.0308556
GAGTACT69000.021.82059512-13
GTACTTT71500.020.89156714-15
GGACCGT2309.0363756E-820.6504046
AGTACTT72550.019.7054112-13
ACGCAGA163900.019.6185137
CATGGGG43200.019.4792464
AGTATCG2702.2775566E-819.3691945
CGCAGAG164450.019.2929658
Produced by FastQC (version 0.11.5)