##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938393_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1885891 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.801669873815612 31.0 31.0 34.0 30.0 34.0 2 32.03145887010437 31.0 31.0 34.0 30.0 34.0 3 32.09633112412117 33.0 31.0 34.0 30.0 34.0 4 35.76320953862127 37.0 35.0 37.0 35.0 37.0 5 35.63698644301288 37.0 35.0 37.0 33.0 37.0 6 35.683129618837995 37.0 35.0 37.0 33.0 37.0 7 35.62349096527848 37.0 35.0 37.0 33.0 37.0 8 35.61508963137318 37.0 35.0 37.0 33.0 37.0 9 37.175125179557035 39.0 37.0 39.0 33.0 39.0 10-11 37.11162654681527 39.0 37.0 39.0 33.0 39.0 12-13 37.00197307267493 39.0 37.0 39.0 33.0 39.0 14-15 38.02011463016685 40.0 37.0 41.0 33.0 41.0 16-17 37.946748247910406 40.0 37.0 41.0 33.0 41.0 18-19 37.96629418137103 40.0 37.0 41.0 33.0 41.0 20-21 37.97555611644576 40.0 37.0 41.0 33.0 41.0 22-23 37.999418842340305 40.0 37.0 41.0 33.0 41.0 24-25 37.92198144007263 40.0 37.0 41.0 33.0 41.0 26-27 37.7426062800024 40.0 37.0 41.0 32.0 41.0 28-29 37.60832015211908 39.5 37.0 41.0 32.0 41.0 30-31 37.36764134300445 39.0 36.0 41.0 31.5 41.0 32-33 37.139284560984706 39.0 36.0 41.0 31.0 41.0 34-35 36.957850162071935 39.0 35.5 40.5 31.0 41.0 36-37 36.96166162307365 39.0 35.5 41.0 31.0 41.0 38-39 36.81296480019259 39.0 35.0 41.0 30.0 41.0 40-41 36.639329102265194 39.0 35.0 40.5 30.0 41.0 42-43 36.3977926083745 39.0 35.0 40.0 30.0 41.0 44-45 36.093123621672724 38.5 35.0 40.0 30.0 41.0 46-47 35.68962071508905 38.0 35.0 40.0 28.5 41.0 48-49 35.27653003275375 38.0 34.0 40.0 27.0 41.0 50-51 34.90537549625084 38.0 34.0 40.0 26.0 41.0 52-53 34.398466295241874 38.0 33.0 40.0 24.0 41.0 54-55 34.08349634204734 38.0 33.0 40.0 23.5 41.0 56-57 33.71865659256023 37.0 33.0 40.0 23.0 41.0 58-59 33.26364964889275 37.0 32.0 39.5 22.0 41.0 60-61 32.837770051397456 36.0 31.0 39.0 20.5 40.5 62-63 32.35282632983561 36.0 31.0 39.0 18.5 40.0 64-65 31.85336586260818 35.0 30.5 38.5 17.5 40.0 66-67 31.344891618868747 35.0 30.0 38.0 13.5 40.0 68-69 30.77491302519605 34.5 29.0 37.5 9.0 40.0 70-71 30.203879227378465 34.0 29.0 37.0 7.5 39.0 72-73 29.60632719494393 34.0 28.0 36.0 6.0 39.0 74-75 28.980377444931865 33.5 27.0 36.0 2.0 38.5 76-77 27.691493304756214 31.5 26.0 34.5 2.0 36.5 78-79 28.30718397828931 33.0 27.0 35.0 2.0 37.0 80-81 27.98368967241479 33.0 26.0 35.0 2.0 37.0 82-83 27.472237260796092 33.0 26.0 35.0 2.0 36.0 84-85 26.870976371380955 32.0 25.0 35.0 2.0 36.0 86-87 26.220101002656037 32.0 24.0 34.5 2.0 35.0 88-89 25.46217570368595 31.5 20.5 34.0 2.0 35.0 90-91 24.90437411281988 31.0 19.0 34.0 2.0 35.0 92-93 24.10863962975591 31.0 13.5 34.0 2.0 35.0 94-95 23.31757667860974 30.5 4.5 34.0 2.0 35.0 96-97 22.32769523795384 30.0 2.0 34.0 2.0 35.0 98-99 21.157741088960073 29.5 2.0 34.0 2.0 35.0 100-101 18.04522186064836 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29698245405859325 1101 2 0.31448977999249905 1101 3 0.2370530175744463 1101 4 0.22272054296126953 1101 5 0.14554942909954605 1101 6 0.1545165262641035 1101 7 0.2797507624487139 1101 8 0.2563767276177771 1101 9 0.23591591529833522 1101 10-11 0.2782235797880048 1101 12-13 0.311845879054367 1101 14-15 0.4888678043510595 1101 16-17 0.45852246874147085 1101 18-19 0.5156545353226889 1101 20-21 0.4787731188521249 1101 22-23 0.5767216841937994 1101 24-25 0.558190355401095 1101 26-27 0.5135560894666966 1101 28-29 0.5003584929770923 1101 30-31 0.5334473403273421 1101 32-33 0.3658481878882114 1101 34-35 0.3853716526885478 1101 36-37 0.6730969471341481 1101 38-39 0.7648448188507686 1101 40-41 0.8478150617966307 1101 42-43 0.7251737667341658 1101 44-45 0.9202227904180518 1101 46-47 0.9564815060339882 1101 48-49 1.131557444282187 1101 50-51 1.181659514504254 1101 52-53 1.1554589350384674 1101 54-55 1.2330389210429686 1101 56-57 1.2616767870001269 1101 58-59 1.3666288832861042 1101 60-61 1.576196237199099 1101 62-63 1.5951889416916458 1101 64-65 1.5155482958914668 1101 66-67 1.6538558134392787 1101 68-69 1.6214477507869312 1101 70-71 1.6821461839833027 1101 72-73 1.9479554114951831 1101 74-75 1.7210324280749916 1101 76-77 1.9187585938367633 1101 78-79 2.34732444271096 1101 80-81 2.6662864560577475 1101 82-83 2.6837121672189177 1101 84-85 2.979478302068408 1101 86-87 3.112126271508753 1101 88-89 3.2162660904967346 1101 90-91 3.263514091401621 1101 92-93 3.585121048199845 1101 94-95 3.6699301190199307 1101 96-97 4.047837812320925 1101 98-99 4.218987737103397 1101 100-101 4.600217017507649 1102 1 0.3065899420558438 1102 2 0.2671883477754591 1102 3 0.2984256015201865 1102 4 0.2093289639012781 1102 5 0.07359643953827089 1102 6 0.09733513033013708 1102 7 0.1416329083228689 1102 8 0.24273001487419776 1102 9 0.20115248124598395 1102 10-11 0.30087360162721666 1102 12-13 0.3207524842538021 1102 14-15 0.43614389955942556 1102 16-17 0.36034479340907666 1102 18-19 0.45799887879141465 1102 20-21 0.48515146094171513 1102 22-23 0.37421020464770294 1102 24-25 0.33253460598704976 1102 26-27 0.36130094872873286 1102 28-29 0.2902708951195052 1102 30-31 0.24104268184494515 1102 32-33 0.2337937268404815 1102 34-35 0.29034611286623857 1102 36-37 0.49167955607006064 1102 38-39 0.7198310657343399 1102 40-41 0.732295497334519 1102 42-43 0.7877607341058663 1102 44-45 0.6246648206943064 1102 46-47 0.603142811366105 1102 48-49 0.7155607974677451 1102 50-51 0.8085410519374747 1102 52-53 0.8957747305764485 1102 54-55 0.7466167065413813 1102 56-57 0.9187221508272287 1102 58-59 0.9814014174984109 1102 60-61 0.9763500522412869 1102 62-63 1.1469966578983346 1102 64-65 1.0497295960033348 1102 66-67 1.0177846135055972 1102 68-69 1.08180870616264 1102 70-71 1.1697858682053983 1102 72-73 1.348874774554531 1102 74-75 1.3448110637446788 1102 76-77 1.4558144099147725 1102 78-79 1.7806112464796442 1102 80-81 1.9402247854259258 1102 82-83 1.7444689619168408 1102 84-85 1.8736275105955151 1102 86-87 2.023339161756006 1102 88-89 2.0960281695188065 1102 90-91 2.356122621684637 1102 92-93 2.427290736789786 1102 94-95 2.50662604375902 1102 96-97 2.9142643064842986 1102 98-99 3.421379292060898 1102 100-101 3.840616436931551 1103 1 0.34034515098061036 1103 2 0.2942575512013903 1103 3 0.34556314466264126 1103 4 0.20507235618019592 1103 5 0.17843734151241364 1103 6 0.23287814890849035 1103 7 0.3672004273433984 1103 8 0.32599280620407 1103 9 0.37183918184162934 1103 10-11 0.4587134850436456 1103 12-13 0.46699293957662746 1103 14-15 0.6229838586794756 1103 16-17 0.6650006516643856 1103 18-19 0.47928965981183325 1103 20-21 0.510807673627582 1103 22-23 0.5254991446938817 1103 24-25 0.5237430077117153 1103 26-27 0.4858157573878472 1103 28-29 0.4985775601344571 1103 30-31 0.4041770867285095 1103 32-33 0.4394881870565186 1103 34-35 0.4822177814771109 1103 36-37 0.6877562163019135 1103 38-39 0.6298112967680112 1103 40-41 0.6557307552601657 1103 42-43 0.7862969875340298 1103 44-45 0.9062762409285057 1103 46-47 0.9829237526373049 1103 48-49 1.145890675963443 1103 50-51 1.1800449214244466 1103 52-53 1.4299334053502832 1103 54-55 1.3256852596044553 1103 56-57 1.5696256906593717 1103 58-59 1.7150864285270515 1103 60-61 1.676351256616961 1103 62-63 1.67263136387583 1103 64-65 1.7342279047157838 1103 66-67 1.8153200307529431 1103 68-69 1.855519852143292 1103 70-71 1.8702920500936564 1103 72-73 1.8621388218761723 1103 74-75 1.987640124985461 1103 76-77 1.8549277313419914 1103 78-79 2.104332371572987 1103 80-81 2.408518735674889 1103 82-83 2.351979276534177 1103 84-85 2.6307819322027015 1103 86-87 3.067198652408333 1103 88-89 3.161563411271416 1103 90-91 3.291043322149523 1103 92-93 3.376721583259414 1103 94-95 3.5961636323236448 1103 96-97 4.127690428360236 1103 98-99 4.252563244959482 1103 100-101 4.559144380167606 1104 1 0.4076314598337518 1104 2 0.3060377605373077 1104 3 0.282344640585535 1104 4 0.18850111594320396 1104 5 0.13207367429060213 1104 6 0.18430511073463407 1104 7 0.2320151752435251 1104 8 0.2623168793960886 1104 9 0.4228457855914556 1104 10-11 0.4128665056761207 1104 12-13 0.40122973070825907 1104 14-15 0.45781346529045663 1104 16-17 0.475743297040502 1104 18-19 0.5938755594755634 1104 20-21 0.5157841646723895 1104 22-23 0.36806045466936865 1104 24-25 0.40265546395407625 1104 26-27 0.43267574606587544 1104 28-29 0.572477884135381 1104 30-31 0.5740771928308845 1104 32-33 0.5112953435460028 1104 34-35 0.4711764015550912 1104 36-37 0.6890781159831718 1104 38-39 0.6782670365336188 1104 40-41 0.724734247111968 1104 42-43 0.861413546839124 1104 44-45 0.9193953219440658 1104 46-47 0.9604676088286368 1104 48-49 1.0326369413025773 1104 50-51 0.8134931442372206 1104 52-53 0.9650268444136927 1104 54-55 1.0866066036991242 1104 56-57 1.1848246353295622 1104 58-59 1.4382693023572202 1104 60-61 1.1531453337180082 1104 62-63 1.1859972232228486 1104 64-65 1.3460607819950958 1104 66-67 1.5987462447161889 1104 68-69 1.4302485191625358 1104 70-71 1.5579634809645135 1104 72-73 1.7081520173311802 1104 74-75 1.9593408232463219 1104 76-77 1.9049180682525844 1104 78-79 2.0895548251983698 1104 80-81 2.2676489590811357 1104 82-83 2.473435003160173 1104 84-85 2.5058832167092397 1104 86-87 2.660531914568768 1104 88-89 2.727151972801476 1104 90-91 2.8980900958821287 1104 92-93 3.1883053006526474 1104 94-95 3.278933166820053 1104 96-97 3.7292477187095194 1104 98-99 4.110756039196453 1104 100-101 4.379322498553318 1105 1 0.3326645623353741 1105 2 0.3244142747553056 1105 3 0.27217859660021304 1105 4 0.19273219961871746 1105 5 0.09796700877875253 1105 6 0.15251883544517142 1105 7 0.14037590261932564 1105 8 0.16070131984242408 1105 9 0.3436832362329696 1105 10-11 0.2993039941115683 1105 12-13 0.33921867335727285 1105 14-15 0.47161678197083745 1105 16-17 0.47223108553093596 1105 18-19 0.4048297645102963 1105 20-21 0.3695502385779079 1105 22-23 0.3256826911518047 1105 24-25 0.2699156791433026 1105 26-27 0.31509526663405296 1105 28-29 0.5149107638102493 1105 30-31 0.5763477160538102 1105 32-33 0.5906097247949589 1105 34-35 0.4844000799811923 1105 36-37 0.7157841250878292 1105 38-39 0.6289592036090639 1105 40-41 0.42399464077256255 1105 42-43 0.5052581875145776 1105 44-45 0.48955609052575966 1105 46-47 0.6639467506438237 1105 48-49 0.7293556927378901 1105 50-51 0.7948100792014614 1105 52-53 1.1027369255525485 1105 54-55 1.1802820246487755 1105 56-57 1.1455778632335623 1105 58-59 1.3769590075776321 1105 60-61 1.4803050012888406 1105 62-63 1.5218400476897003 1105 64-65 1.6144810610665736 1105 66-67 1.645157067429821 1105 68-69 1.662679784721366 1105 70-71 1.6576941991674126 1105 72-73 1.9661359207886662 1105 74-75 1.9896372563123421 1105 76-77 2.1499768391360305 1105 78-79 2.282038162402955 1105 80-81 2.5409233968635263 1105 82-83 2.567526026181671 1105 84-85 2.5870302101884732 1105 86-87 2.7699729186276763 1105 88-89 3.003282346134707 1105 90-91 3.1214172220863183 1105 92-93 3.476106520294472 1105 94-95 3.5443857032787385 1105 96-97 3.8182015221836743 1105 98-99 4.370077952986907 1105 100-101 4.658814563795101 1106 1 0.19357319491087566 1106 2 0.14724395452373074 1106 3 0.12626715359016316 1106 4 0.14281914538062068 1106 5 0.024272281206606294 1106 6 0.0990402809694757 1106 7 0.12756269054933256 1106 8 0.03377709363337544 1106 9 0.21725317261315524 1106 10-11 0.18454269675706314 1106 12-13 0.16552815814605282 1106 14-15 0.13958072416144773 1106 16-17 0.15888645780491117 1106 18-19 0.2771866725847332 1106 20-21 0.4016351334465966 1106 22-23 0.28018656060020675 1106 24-25 0.17240360480566608 1106 26-27 0.1975743463385058 1106 28-29 0.33117732200862093 1106 30-31 0.368522465846155 1106 32-33 0.4056779979507681 1106 34-35 0.3648867129752986 1106 36-37 0.4608982471594274 1106 38-39 0.5403256013428148 1106 40-41 0.521517246012472 1106 42-43 0.680130401597296 1106 44-45 0.11573828303328781 1106 46-47 -0.0718858290718174 1106 48-49 0.4206087253086537 1106 50-51 0.38892936009880685 1106 52-53 0.4935675373058288 1106 54-55 0.6073707962058705 1106 56-57 0.5924556481635719 1106 58-59 0.9949935726316212 1106 60-61 0.8934013629937425 1106 62-63 1.0635771726743926 1106 64-65 0.643989965602632 1106 66-67 0.8583629505183765 1106 68-69 0.8577353944671948 1106 70-71 1.006094620930046 1106 72-73 1.3713572737726665 1106 74-75 1.6224353565375722 1106 76-77 1.60346868887585 1106 78-79 1.7737766695685764 1106 80-81 1.8176926213547162 1106 82-83 1.9459700590433506 1106 84-85 2.1419068735773976 1106 86-87 2.4732719870377373 1106 88-89 2.707362544220981 1106 90-91 2.5659633336164696 1106 92-93 2.294911684122404 1106 94-95 3.0793891060765333 1106 96-97 3.6537221239194295 1106 98-99 3.87361159724513 1106 100-101 4.320024248539376 1107 1 0.13940247582137744 1107 2 0.1641089312438595 1107 3 0.07814170092591155 1107 4 0.14154263218869545 1107 5 -0.001910853816667668 1107 6 -0.021042233257119847 1107 7 0.04962399422237951 1107 8 0.1217178592826258 1107 9 0.08409926676732482 1107 10-11 0.1101036124321837 1107 12-13 0.22383635783564415 1107 14-15 0.2825448990191788 1107 16-17 0.3169550041059992 1107 18-19 0.23664528613879554 1107 20-21 0.3405470579163321 1107 22-23 0.16918332728411656 1107 24-25 0.27087941960131445 1107 26-27 0.23984335823710268 1107 28-29 0.12230898263976542 1107 30-31 0.00304173641004013 1107 32-33 0.052516849476894834 1107 34-35 0.2277544056809333 1107 36-37 0.27435918870106946 1107 38-39 0.1499827405047398 1107 40-41 0.0888947679990224 1107 42-43 0.2623835055807362 1107 44-45 0.5259880243679973 1107 46-47 0.5213335190916624 1107 48-49 0.5639771940101568 1107 50-51 0.4755422674966283 1107 52-53 0.6720002843311761 1107 54-55 0.631325917778554 1107 56-57 0.640866667304806 1107 58-59 0.6189335622850152 1107 60-61 0.624327902052201 1107 62-63 0.8221568415828315 1107 64-65 1.0554587964355342 1107 66-67 1.1130641921117501 1107 68-69 1.0447268585127212 1107 70-71 1.114881222068174 1107 72-73 1.2576085670991013 1107 74-75 1.2433020145820635 1107 76-77 1.294609790789977 1107 78-79 1.5810839639296752 1107 80-81 1.898332429942407 1107 82-83 1.9786411661307781 1107 84-85 2.2412617623513214 1107 86-87 2.335938567482639 1107 88-89 2.5368128804858507 1107 90-91 2.5109286724679976 1107 92-93 2.741782092813555 1107 94-95 2.747711403024283 1107 96-97 3.1421601735831644 1107 98-99 3.5621237545136246 1107 100-101 3.793742044711127 1108 1 0.18719422434789834 1108 2 0.15320519843442781 1108 3 0.1329246085879987 1108 4 0.12165559878987153 1108 5 0.11012450964108922 1108 6 0.03018271762697111 1108 7 0.11937153842670511 1108 8 0.08717267264215423 1108 9 0.05347942393824212 1108 10-11 0.15676323915123902 1108 12-13 0.2329488332776961 1108 14-15 0.1416671976439332 1108 16-17 0.2892644343613995 1108 18-19 0.3151413568657091 1108 20-21 0.40965756872183334 1108 22-23 0.340264368541483 1108 24-25 0.2857148812909003 1108 26-27 0.2305840262135277 1108 28-29 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1.745725787377392 2316 86-87 1.7519516018934844 2316 88-89 1.9752565718310038 2316 90-91 2.244542853906996 2316 92-93 2.269768015649845 2316 94-95 2.8721212049012372 2316 96-97 2.944722999443435 2316 98-99 2.943185276932809 2316 100-101 3.4449208768279185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 31.0 5 74.0 6 164.0 7 369.0 8 837.0 9 1439.0 10 2474.0 11 3826.0 12 5497.0 13 7804.0 14 10367.0 15 13626.0 16 17474.0 17 22149.0 18 21633.0 19 17889.0 20 18200.0 21 19458.0 22 21273.0 23 23679.0 24 27223.0 25 31897.0 26 37173.0 27 44306.0 28 52623.0 29 62228.0 30 73227.0 31 86373.0 32 102299.0 33 124095.0 34 153515.0 35 194295.0 36 244903.0 37 278273.0 38 156267.0 39 8923.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.799436021352285 19.650275970935695 15.424041843440248 22.126246164271773 2 15.852355615441335 21.009458534696325 39.512219705369034 23.625966144493308 3 19.489853326468314 25.421909773056633 29.696710675972977 25.391526224502076 4 13.270703343936633 17.405671907867422 36.50794239964028 32.81568234855567 5 13.639813918331296 38.001670998097566 33.6302168263351 14.72829825723604 6 31.490367152714555 36.38312076360723 17.413837809290143 14.712674274388075 7 28.637551162819058 32.84479325687434 20.37349984702191 18.14415573328469 8 26.65032072373218 35.10494508961546 19.25943758149331 18.985296605159046 9 26.69841470159198 16.94111695744876 19.92676140879828 36.43370693216098 10-11 25.16166098676965 27.060047478883988 26.792057441283724 20.98623409306264 12-13 26.33293758759122 24.293212067929694 27.443924383752826 21.92992596072626 14-15 22.97494924149911 25.581727682034643 26.21246402893911 25.230859047527137 16-17 21.92181308463745 28.901378711707093 26.83625405710086 22.3405541465546 18-19 22.025769251775422 27.290363016738507 29.32507764234518 21.35879008914089 20-21 23.040859731553944 26.24197262726213 29.28628961058725 21.430878030596677 22-23 22.628137045036006 26.181762360603024 29.2466531734867 21.94344742087427 24-25 22.391151033740396 26.43724116720665 29.4080185802839 21.763589218769056 26-27 22.085897866951484 26.55659191645569 29.465258281194153 21.892251935398676 28-29 21.86078092530268 26.880927900923226 29.46501149854366 21.793279675230433 30-31 22.09308491317897 26.48917673396819 29.352650815980354 22.06508753687249 32-33 21.967711120767664 26.591145838166398 29.166148562356646 22.274994478709292 34-35 22.500452112891924 26.576707249875504 29.072317379537772 21.8505232576948 36-37 22.520382326037673 26.248485410894435 29.225140190632747 22.00599207243514 38-39 22.173724202447545 26.57431625481798 29.182841511971507 22.069118030762965 40-41 22.300481558295278 26.53186817149436 29.101982072943805 22.065668197266554 42-43 22.081393375685394 26.545010552662557 28.86442214892511 22.509173922726937 44-45 22.180366404555212 26.667546911480777 28.800019513860942 22.35206717010307 46-47 22.482956972270628 26.959229031833004 28.270812404869112 22.287001591027252 48-49 22.321305950343895 26.86671711143433 28.329580023447804 22.48239691477397 50-51 22.370195308212402 27.0054844102867 28.27902036756101 22.345299913939883 52-53 22.557958015601116 27.246007324919624 27.80632602838658 22.389708631092677 54-55 22.164350961959094 27.024387941826966 28.105547987662067 22.705713108551873 56-57 22.34699672462512 27.136006269715484 27.99435386244486 22.522643143214534 58-59 22.4475062450587 26.811438200829212 28.031232982181898 22.70982257193019 60-61 22.30531881216889 26.994136988829155 28.337242184198345 22.36330201480361 62-63 22.20191941103701 27.086188968503482 28.213560592844445 22.498331027615066 64-65 21.988969670039253 27.053657926147373 28.077603636689503 22.879768767123867 66-67 22.02051926242645 27.060589277244418 27.895766791903963 23.02312466842517 68-69 22.053510070587436 26.903983693623374 28.10941052703024 22.933095708758948 70-71 22.11365874273752 27.01370333704334 27.83580811404265 23.0368298061765 72-73 22.226205013969526 26.95850396443909 27.766610053285156 23.04868096830623 74-75 21.954476690328338 27.375097500332735 27.51906128190866 23.15136452743027 76-77 22.02234911773798 26.97409341260974 27.866430244377856 23.13712722527442 78-79 22.10154245393822 27.033667375261878 27.83824727940268 23.026542891397224 80-81 22.012359678358845 27.235515529666227 27.780589594146637 22.971535197828295 82-83 22.065348054029148 27.121685417507347 27.763669943490303 23.049296584973202 84-85 21.98450213383335 26.997048480014936 27.721300365678797 23.297149020472922 86-87 21.936414223310084 27.01756544222218 27.712022406729652 23.333997927738086 88-89 21.887378933188327 27.08317370494204 27.528162425124897 23.50128493674474 90-91 21.86460940165796 27.017749637501424 27.723445339139317 23.39419562170129 92-93 21.971644650065286 26.877610674019525 27.63995731452698 23.510787361388203 94-95 21.685214044714144 26.73563848599946 27.701203303902506 23.877944165383898 96-97 21.65290084445518 26.716927626497935 27.77791181407404 23.85225971497285 98-99 21.607823569866973 26.83421258174518 27.392383759188625 24.165580089199217 100-101 21.61746626434248 26.512540196847567 27.701218876855872 24.16877466195409 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1221.0 1 814.5 2 596.0 3 757.0 4 596.5 5 545.0 6 923.5 7 2070.5 8 3226.5 9 3431.0 10 2885.0 11 2318.0 12 2324.5 13 2456.5 14 2574.5 15 2668.5 16 2707.5 17 2871.0 18 3028.0 19 3247.0 20 3511.5 21 3841.5 22 4512.5 23 5323.0 24 6131.5 25 7260.0 26 8789.0 27 10977.0 28 13420.5 29 16325.0 30 20047.5 31 23397.0 32 27251.0 33 31050.0 34 34238.0 35 37703.0 36 42267.5 37 46610.5 38 49552.0 39 51889.5 40 54483.0 41 58316.0 42 61437.0 43 64180.5 44 67188.0 45 70110.5 46 71644.5 47 74308.5 48 79856.5 49 79236.5 50 73483.0 51 68933.0 52 65797.5 53 63938.0 54 62252.0 55 58633.0 56 52624.5 57 47393.5 58 43247.5 59 38274.0 60 31889.0 61 26186.0 62 21530.0 63 16378.5 64 12024.5 65 9101.0 66 6899.5 67 5307.0 68 3945.0 69 2833.0 70 2168.0 71 1737.5 72 1426.5 73 1242.0 74 977.0 75 755.5 76 548.5 77 318.0 78 206.0 79 141.5 80 95.5 81 46.5 82 17.5 83 14.5 84 7.5 85 2.5 86 1.5 87 1.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.1210133565513595E-4 2 0.04353379914321666 3 1.0605066782756797E-4 4 0.0 5 0.10536133848668879 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011135320121894638 26-27 0.012354902801911668 28-29 0.0 30-31 0.0 32-33 5.03740672180948E-4 34-35 0.015907600174135197 36-37 0.0034996720383097435 38-39 0.013097257476704646 40-41 0.00731749608010219 42-43 0.005885812064430023 44-45 0.0027573173635167675 46-47 1.3256333478445996E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.3861400261202795E-4 68-69 1.5907600174135196E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.6512666956891994E-5 82-83 0.0010605066782756797 84-85 0.004401102714844071 86-87 0.0015377346834997356 88-89 2.6512666956891994E-5 90-91 0.0011400446791463557 92-93 1.8558866869824395E-4 94-95 0.0 96-97 2.6512666956891994E-5 98-99 0.0 100-101 8.218926756636518E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1885891.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.75178670558994 #Duplication Level Percentage of deduplicated Percentage of total 1 76.89136495529613 42.09939613534047 2 12.953597359998176 14.184651994494264 3 4.222937505868071 6.936401207769737 4 1.878800332782864 4.114707003316751 5 1.0244028629535185 2.8043943526513364 6 0.6480852738457271 2.1290296008381064 7 0.41823151718315904 1.602924597565732 8 0.30998102961288665 1.3577612172915143 9 0.21928613568726038 1.080567695577774 >10 1.2880735132326564 12.981046263406784 >50 0.09826737270302928 3.749288256043787 >100 0.0431880224179625 4.230325666317871 >500 0.0029108603220581774 1.0996861440333496 >1k 5.821720644116354E-4 0.5368274630655554 >5k 2.910860322058177E-4 1.0929924022870596 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8397 0.4452537288740442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6934 0.36767766535817814 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5243 0.27801182570996946 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2663 0.14120646421240676 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1910 0.10127838777532741 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.302533391378399E-5 0.0 12-13 0.0 0.0 0.0 5.302533391378399E-5 0.0 14-15 0.0 0.0 0.0 7.953800087067598E-5 0.0 16-17 0.0 0.0 0.0 1.0605066782756797E-4 0.0 18-19 0.0 0.0 0.0 1.0605066782756797E-4 0.0 20-21 0.0 0.0 0.0 1.3256333478445996E-4 0.0 22-23 0.0 0.0 0.0 7.423546747929758E-4 0.0 24-25 0.0 0.0 0.0 0.0015907600174135195 0.0 26-27 0.0 0.0 0.0 0.0024391653600340634 0.0 28-29 0.0 0.0 0.0 0.005806274063559347 0.0 30-31 0.0 0.0 0.0 0.015006169497600868 0.0 32-33 0.0 0.0 0.0 0.0340952897065631 0.0 34-35 0.0 0.0 0.0 0.06071400733128267 0.0 36-37 0.0 0.0 0.0 0.1059711298266973 0.0 38-39 0.0 0.0 0.0 0.17432078524156486 0.0 40-41 0.0 0.0 0.0 0.24691246736953515 0.0 42-43 0.0 0.0 0.0 0.32706025958021967 0.0 44-45 0.0 0.0 0.0 0.4179721945754023 0.0 46-47 5.302533391378399E-5 0.0 0.0 0.5296700604647883 0.0 48-49 5.302533391378399E-5 0.0 0.0 0.6490831124386298 0.0 50-51 5.302533391378399E-5 0.0 0.0 0.7791542565291419 0.0 52-53 5.302533391378399E-5 0.0 0.0 0.9078202292709387 0.0 54-55 5.302533391378399E-5 0.0 0.0 1.0491857694850868 0.0 56-57 5.302533391378399E-5 0.0 0.0 1.2173556159926529 0.0 58-59 5.302533391378399E-5 0.0 0.0 1.406337906061379 0.0 60-61 5.302533391378399E-5 0.0 0.0 1.6145683923408087 0.0 62-63 5.302533391378399E-5 0.0 0.0 1.81672747788711 0.0 64-65 1.0605066782756797E-4 0.0 0.0 2.0243481728265316 0.0 66-67 1.0605066782756797E-4 0.0 0.0 2.262537972767249 0.0 68-69 1.0605066782756797E-4 0.0 0.0 2.5305014977005564 5.302533391378399E-5 70-71 1.0605066782756797E-4 0.0 0.0 2.8260912216029452 5.302533391378399E-5 72-73 1.0605066782756797E-4 0.0 0.0 3.123324730856661 5.302533391378399E-5 74-75 1.0605066782756797E-4 0.0 0.0 3.4279287615243934 5.302533391378399E-5 76-77 1.0605066782756797E-4 0.0 0.0 3.756447217787242 5.302533391378399E-5 78-79 1.0605066782756797E-4 0.0 0.0 4.125238415157609 5.302533391378399E-5 80-81 1.0605066782756797E-4 0.0 0.0 4.517572860785697 5.302533391378399E-5 82-83 1.0605066782756797E-4 0.0 0.0 4.917781568499983 5.302533391378399E-5 84-85 1.0605066782756797E-4 0.0 0.0 5.320668055576913 5.302533391378399E-5 86-87 1.0605066782756797E-4 0.0 0.0 5.730686450065248 5.302533391378399E-5 88-89 1.0605066782756797E-4 0.0 0.0 6.189991892426445 5.302533391378399E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8170 0.0 29.630552 1 GTATCAA 11640 0.0 27.947771 1 TACATGG 10590 0.0 24.476233 2 GTACATG 10720 0.0 24.22378 1 TATCAAC 13925 0.0 23.259262 2 ATCAACG 13880 0.0 23.223244 3 TCAACGC 14140 0.0 22.86347 4 ACATGGG 10960 0.0 22.643452 3 TACCTGG 1640 0.0 22.620115 2 CAACGCA 14295 0.0 22.582306 5 TAAGGTG 700 0.0 22.412924 5 AACGCAG 14660 0.0 22.030855 6 GAGTACT 6900 0.0 21.820595 12-13 GTACTTT 7150 0.0 20.891567 14-15 GGACCGT 230 9.0363756E-8 20.650404 6 AGTACTT 7255 0.0 19.70541 12-13 ACGCAGA 16390 0.0 19.618513 7 CATGGGG 4320 0.0 19.479246 4 AGTATCG 270 2.2775566E-8 19.369194 5 CGCAGAG 16445 0.0 19.292965 8 >>END_MODULE