FastQCFastQC Report
Thu 26 May 2016
SRR938392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901615
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84240.4429918779563687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67390.35438298498907506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47960.2522066769561662No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26170.1376198652198263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84750.029.1516041
GTATCAA120600.028.5536941
GTACATG105300.025.5913071
TACATGG105200.025.0754992
ACATGGG108350.023.5130083
TATCAAC146150.023.448162
ATCAACG146050.023.334043
TCAACGC147750.023.001224
CAACGCA148500.022.7884315
TACCTGG16650.022.5520062
AACGCAG152750.022.2113536
GAGTACT73900.022.20789512-13
GGACCGA5000.020.9079136
GTACTTT79150.020.52669114-15
AGTACTT77600.020.4144612-13
CATGGGG47150.020.0605934
ACGATAC2651.843E-819.7296373
ACGCAGA170350.019.6871787
CGCAGAG170500.019.6141368
CATGGGA60600.019.372994
Produced by FastQC (version 0.11.5)