##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938392_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1901615 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.116883806659075 31.0 31.0 34.0 28.0 34.0 2 31.281519129792308 31.0 31.0 34.0 28.0 34.0 3 31.324588310462424 31.0 31.0 34.0 28.0 34.0 4 34.996394117631596 37.0 35.0 37.0 32.0 37.0 5 34.999625581413696 37.0 35.0 37.0 32.0 37.0 6 34.97809335748824 37.0 35.0 37.0 32.0 37.0 7 34.90222100688099 37.0 35.0 37.0 32.0 37.0 8 34.86320417119133 37.0 35.0 37.0 32.0 37.0 9 36.333954033808105 39.0 35.0 39.0 32.0 39.0 10-11 36.16742978994171 39.0 35.0 39.0 31.5 39.0 12-13 35.98149309928666 38.0 35.0 39.0 30.0 39.0 14-15 37.11166771402203 39.0 36.0 41.0 31.0 41.0 16-17 37.05514838702892 39.0 36.0 41.0 31.0 41.0 18-19 37.07420245422969 39.0 36.0 41.0 31.0 41.0 20-21 37.01593592814528 39.0 36.0 41.0 31.0 41.0 22-23 36.85800359168391 39.0 36.0 41.0 30.0 41.0 24-25 36.71638922705174 39.0 36.0 41.0 30.0 41.0 26-27 36.49381446822832 39.0 36.0 41.0 30.0 41.0 28-29 36.26479176910153 39.0 35.0 40.5 29.0 41.0 30-31 35.987407019822626 38.5 35.0 40.0 28.5 41.0 32-33 35.68175655955596 38.0 35.0 40.0 27.0 41.0 34-35 35.3706578355766 38.0 34.0 40.0 26.5 41.0 36-37 35.146807581976375 38.0 34.0 40.0 25.5 41.0 38-39 34.91226141989835 38.0 34.0 40.0 25.0 41.0 40-41 34.6329961637871 38.0 33.5 40.0 24.0 41.0 42-43 34.20939648667054 38.0 33.0 40.0 23.5 41.0 44-45 33.71173213295015 38.0 33.0 40.0 21.5 41.0 46-47 33.1836857092524 37.5 31.5 40.0 18.0 41.0 48-49 32.6695950547298 37.0 31.0 40.0 14.5 41.0 50-51 31.935860045277302 36.5 30.5 39.0 12.0 40.5 52-53 31.93958240758513 36.5 31.0 39.0 9.0 40.0 54-55 31.932245223139276 36.5 30.5 39.0 8.0 40.5 56-57 31.52026014729585 36.0 30.0 39.0 4.5 40.5 58-59 31.067273869842214 36.0 29.0 39.0 2.0 40.0 60-61 30.5106467397449 35.0 28.5 39.0 2.0 40.0 62-63 29.795649487409385 35.0 27.5 38.0 2.0 40.0 64-65 29.137224411881476 34.0 26.5 38.0 2.0 40.0 66-67 28.43699802536265 34.0 26.0 37.5 2.0 40.0 68-69 27.598981129198076 33.5 24.0 37.0 2.0 39.0 70-71 26.775440086452832 32.5 22.0 36.0 2.0 39.0 72-73 25.881947712865117 32.0 19.0 35.5 2.0 38.5 74-75 24.944096728307255 31.0 13.5 35.0 2.0 37.5 76-77 24.00221942927459 30.5 7.5 34.5 2.0 37.0 78-79 23.022032588089598 29.5 2.0 34.0 2.0 36.0 80-81 22.051687907383986 29.0 2.0 34.0 2.0 36.0 82-83 21.051067119264417 28.0 2.0 34.0 2.0 35.0 84-85 20.075044896048887 26.5 2.0 33.0 2.0 35.0 86-87 19.08445452943945 25.5 2.0 32.5 2.0 35.0 88-89 17.992994112898774 23.0 2.0 32.0 2.0 34.0 90-91 16.845303597205532 19.0 2.0 31.5 2.0 34.0 92-93 15.683789305406194 6.0 2.0 31.0 2.0 34.0 94-95 14.432477920083718 2.0 2.0 30.5 2.0 34.0 96-97 13.015587802999029 2.0 2.0 29.5 2.0 34.0 98-99 11.331965460937152 2.0 2.0 28.0 2.0 34.0 100-101 7.986094188360946 2.0 2.0 10.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5918065790907008 1101 2 0.5006337224962749 1101 3 0.4723827322143137 1101 4 0.45004770913800485 1101 5 0.3944520727567635 1101 6 0.5762419068241016 1101 7 0.23388430718418363 1101 8 0.3827315601846806 1101 9 0.4795765698277066 1101 10-11 0.6215730249596803 1101 12-13 0.6436569534210861 1101 14-15 0.7028730019860348 1101 16-17 0.8145238811348392 1101 18-19 0.7753115113347562 1101 20-21 0.8401132056391987 1101 22-23 0.9570724978235745 1101 24-25 0.8066235208504153 1101 26-27 0.8590195435369168 1101 28-29 1.0103434853432631 1101 30-31 1.082956416721693 1101 32-33 1.094539713854246 1101 34-35 1.2390792967505888 1101 36-37 1.2653873297171856 1101 38-39 1.2737452925670425 1101 40-41 1.2725265626296505 1101 42-43 1.1429384204080506 1101 44-45 1.239925610936318 1101 46-47 1.2818190166921042 1101 48-49 1.1999453811995338 1101 50-51 1.1969827979479568 1101 52-53 1.3575790659374007 1101 54-55 1.6618676553268443 1101 56-57 1.8663132574121555 1101 58-59 1.8120004131229095 1101 60-61 1.7112242069992583 1101 62-63 1.81801225510738 1101 64-65 1.9939977106443258 1101 66-67 2.1911035000267916 1101 68-69 2.2877732379586746 1101 70-71 2.3834444483287562 1101 72-73 2.4376310708246756 1101 74-75 2.5113144486667025 1101 76-77 2.6643294381039837 1101 78-79 3.0922310612335586 1101 80-81 3.487412411574468 1101 82-83 3.7040075933902585 1101 84-85 3.8407821834737383 1101 86-87 3.730207147289196 1101 88-89 3.91629471510581 1101 90-91 3.9293978477655145 1101 92-93 3.970870159217162 1101 94-95 3.802704201630064 1101 96-97 3.5255286988000503 1101 98-99 3.0676807098118974 1101 100-101 2.2461616532302253 1102 1 0.6657407399109907 1102 2 0.5999321574665935 1102 3 0.49666767554944613 1102 4 0.37017722883579296 1102 5 0.4716242260217385 1102 6 0.5309100125985182 1102 7 0.583587491210082 1102 8 0.5376155321220821 1102 9 0.5961442978363394 1102 10-11 0.7386804183757718 1102 12-13 0.8139213894707353 1102 14-15 0.9028190354452974 1102 16-17 0.9526782254413675 1102 18-19 0.8862127525651928 1102 20-21 0.8646679816780605 1102 22-23 0.9028361243751064 1102 24-25 0.7504983184758842 1102 26-27 0.8752095057603384 1102 28-29 0.6557833126503283 1102 30-31 0.7343325635106837 1102 32-33 0.6843940041942318 1102 34-35 0.7061597068962939 1102 36-37 0.8333851710555606 1102 38-39 1.0192930529556037 1102 40-41 1.115753761766186 1102 42-43 1.3037587118273706 1102 44-45 1.234528956861844 1102 46-47 1.4294175056289689 1102 48-49 1.4635719327375867 1102 50-51 1.3796595383688945 1102 52-53 1.3411192710102569 1102 54-55 1.3623533541935515 1102 56-57 1.7038739697704948 1102 58-59 1.5605163332524299 1102 60-61 1.4783585836857078 1102 62-63 1.6706835988742412 1102 64-65 1.7508784445892793 1102 66-67 1.807401395331702 1102 68-69 2.193061958951656 1102 70-71 2.225052651242951 1102 72-73 2.195321302880803 1102 74-75 2.3674936175819745 1102 76-77 2.540206394391088 1102 78-79 2.921157127072739 1102 80-81 3.314179815783856 1102 82-83 3.3124803402871805 1102 84-85 3.388272739329107 1102 86-87 3.377265970818609 1102 88-89 3.367724828435545 1102 90-91 3.3233535952021036 1102 92-93 3.4063801430271976 1102 94-95 3.3507344228928773 1102 96-97 3.287266421412033 1102 98-99 2.8782581517978656 1102 100-101 2.1916554470780385 1103 1 0.46735751716117946 1103 2 0.4321041812915283 1103 3 0.3965997124151066 1103 4 0.41406736012782375 1103 5 0.3714333659680449 1103 6 0.34314030435637477 1103 7 0.4400968033543222 1103 8 0.34365204673130734 1103 9 0.5401747079962789 1103 10-11 0.6446490551957424 1103 12-13 0.46530237248109074 1103 14-15 0.6236204200839168 1103 16-17 0.6871221638210798 1103 18-19 0.681665338962155 1103 20-21 0.7897251842198685 1103 22-23 0.7478348029487449 1103 24-25 0.8178091318970289 1103 26-27 0.8811762649884969 1103 28-29 0.8196157165282969 1103 30-31 0.7402845295911291 1103 32-33 1.0022446963855316 1103 34-35 0.9472918549381504 1103 36-37 1.0187222599518577 1103 38-39 1.0005458675653145 1103 40-41 1.2309401796083108 1103 42-43 1.0700082022461004 1103 44-45 1.12681891052231 1103 46-47 1.2854833078258068 1103 48-49 1.0337982764319023 1103 50-51 1.2428351549373282 1103 52-53 1.4335403147180301 1103 54-55 1.5587985323528315 1103 56-57 1.4669163342161902 1103 58-59 1.5545683499425138 1103 60-61 1.662865264925525 1103 62-63 2.084517069565745 1103 64-65 2.157786588530019 1103 66-67 2.2317094102875714 1103 68-69 2.2440580640865626 1103 70-71 1.9432488772264875 1103 72-73 2.314612676967929 1103 74-75 2.385844090578651 1103 76-77 2.5491777272622294 1103 78-79 2.688331473706345 1103 80-81 2.855391107165911 1103 82-83 3.0449080010837193 1103 84-85 3.1485642895115937 1103 86-87 3.045808712062847 1103 88-89 3.045618403686152 1103 90-91 2.809623704992866 1103 92-93 2.9523437331079396 1103 94-95 2.9917094221887837 1103 96-97 2.977743275683311 1103 98-99 2.5464249479519907 1103 100-101 1.8515853047992517 1104 1 0.5877570160870427 1104 2 0.4296932816064931 1104 3 0.4722426347429476 1104 4 0.4500305769028472 1104 5 0.44096968345103704 1104 6 0.5028888603672783 1104 7 0.5080837965883092 1104 8 0.4326153787609428 1104 9 0.4990741587419336 1104 10-11 0.5848640333823951 1104 12-13 0.6799895884776745 1104 14-15 0.6439401854935554 1104 16-17 0.7474929057773565 1104 18-19 0.682532582657629 1104 20-21 0.6725783215070038 1104 22-23 0.7740627841225702 1104 24-25 0.7702448774526403 1104 26-27 0.822003415195482 1104 28-29 0.8175042898840772 1104 30-31 0.9021057791400366 1104 32-33 0.9093192510020458 1104 34-35 0.9919993066209614 1104 36-37 1.0100649383555975 1104 38-39 0.8084712279249118 1104 40-41 0.6732278513329533 1104 42-43 0.8888903081178512 1104 44-45 0.732636812019301 1104 46-47 0.7219241699302827 1104 48-49 0.6500675036403081 1104 50-51 0.7874519144689778 1104 52-53 0.8771741980614749 1104 54-55 1.0815225844781509 1104 56-57 1.292585236066497 1104 58-59 1.4209835348316915 1104 60-61 1.6026768519814816 1104 62-63 1.5135617978010458 1104 64-65 1.5058762906251992 1104 66-67 1.674101000102091 1104 68-69 1.9386975911269921 1104 70-71 2.1690761124573648 1104 72-73 2.345644508023568 1104 74-75 2.329545993035463 1104 76-77 2.6402373705775553 1104 78-79 2.777799280826816 1104 80-81 2.9102532456490486 1104 82-83 2.8804122777854744 1104 84-85 2.992277150603339 1104 86-87 3.2881787624914587 1104 88-89 3.46559769564497 1104 90-91 3.3460394378137863 1104 92-93 3.263491498963477 1104 94-95 3.405858909305774 1104 96-97 3.435982374203329 1104 98-99 3.139944615867563 1104 100-101 2.3688035875811595 1105 1 0.4059222518304004 1105 2 0.3064807067647166 1105 3 0.35292606265615944 1105 4 0.30755040814678125 1105 5 0.4037545518089374 1105 6 0.3361480666725001 1105 7 0.3124515732457738 1105 8 0.2878619811427896 1105 9 0.45925280876068086 1105 10-11 0.4139920485607078 1105 12-13 0.4920231089009022 1105 14-15 0.521937834644767 1105 16-17 0.5892745210842136 1105 18-19 0.6895416618602255 1105 20-21 0.5349103293507511 1105 22-23 0.4137492824212714 1105 24-25 0.5812067254485029 1105 26-27 0.4821986500048041 1105 28-29 0.493574998530697 1105 30-31 0.6306604935543376 1105 32-33 0.6559511284184509 1105 34-35 0.7492816726418638 1105 36-37 0.8423846848385708 1105 38-39 0.9089412941890345 1105 40-41 0.943525914431234 1105 42-43 1.0573818192147826 1105 44-45 1.1020346909772343 1105 46-47 1.1563980763108148 1105 48-49 1.1437207811286925 1105 50-51 0.9389406516885366 1105 52-53 1.1793371807571447 1105 54-55 1.4047269057345915 1105 56-57 1.3917662941140456 1105 58-59 1.5379647821166103 1105 60-61 1.5215065919004012 1105 62-63 1.4500407562037516 1105 64-65 1.5776159885853716 1105 66-67 1.888663170686364 1105 68-69 2.162791747200476 1105 70-71 2.19793494173674 1105 72-73 2.0679298611178965 1105 74-75 1.9876549336347438 1105 76-77 2.3221135321273856 1105 78-79 2.3907417046952517 1105 80-81 2.726709161283434 1105 82-83 2.8766923428930653 1105 84-85 2.7239382144051056 1105 86-87 2.8184716198801105 1105 88-89 2.9388635824383833 1105 90-91 2.811814872504769 1105 92-93 2.723107016428349 1105 94-95 2.6623257486509004 1105 96-97 2.9256531027206574 1105 98-99 2.669699696544418 1105 100-101 2.1227128504066224 1106 1 0.27553912644980016 1106 2 0.23696178371370635 1106 3 0.13503124398317112 1106 4 0.2727900396623113 1106 5 0.21191536859026883 1106 6 -0.18711232124680066 1106 7 0.269256745503192 1106 8 -0.12790925789272478 1106 9 0.16497986593796554 1106 10-11 0.21272334253202274 1106 12-13 0.3451563929993142 1106 14-15 0.4622997112724292 1106 16-17 -0.06151414452001802 1106 18-19 0.3332108291423239 1106 20-21 0.39905435443464654 1106 22-23 0.26737996873007575 1106 24-25 0.3376870768303277 1106 26-27 0.36096635190035187 1106 28-29 0.3866298954612901 1106 30-31 0.30094346475191713 1106 32-33 0.37718407435073686 1106 34-35 0.23335067926266362 1106 36-37 0.4795820520662559 1106 38-39 0.6696351177984781 1106 40-41 0.63963298741551 1106 42-43 0.13419930020103976 1106 44-45 0.7325753680868843 1106 46-47 0.926431633792582 1106 48-49 0.7118560734933936 1106 50-51 0.5326907974498063 1106 52-53 0.9346309706795353 1106 54-55 0.7869591908824987 1106 56-57 1.34691782104424 1106 58-59 1.3453125749671813 1106 60-61 1.6269353891432523 1106 62-63 1.6806845675044606 1106 64-65 1.4520120186964078 1106 66-67 1.6807113431640452 1106 68-69 1.7731463681564144 1106 70-71 1.749523156452799 1106 72-73 1.9931847813702923 1106 74-75 2.1617611255731752 1106 76-77 2.453042128543604 1106 78-79 2.624198902710308 1106 80-81 2.2469041574612483 1106 82-83 2.338550053987767 1106 84-85 2.496786583822445 1106 86-87 2.324187663984233 1106 88-89 2.299438335292063 1106 90-91 2.238459383459535 1106 92-93 2.4508931295909147 1106 94-95 2.240264360485419 1106 96-97 2.3884838512209345 1106 98-99 2.1116871027713398 1106 100-101 1.44674324648856 1107 1 0.31859379646692787 1107 2 0.2702571118213193 1107 3 0.29007081192376205 1107 4 0.3451494890866016 1107 5 0.42248757226827394 1107 6 0.3746954273186205 1107 7 0.34131140301860086 1107 8 0.39267682269335324 1107 9 0.5613883721075652 1107 10-11 0.44738410838732534 1107 12-13 0.48482034679296504 1107 14-15 0.5024636088083483 1107 16-17 0.4607624185073931 1107 18-19 0.48171787386097975 1107 20-21 0.6662285908921604 1107 22-23 0.619355490364299 1107 24-25 0.6391374012499327 1107 26-27 0.6326133283596107 1107 28-29 0.7289453388347553 1107 30-31 0.7001097251127177 1107 32-33 0.8834834405135865 1107 34-35 0.7613399027808967 1107 36-37 0.8756231327148498 1107 38-39 0.9217624103011985 1107 40-41 1.1472207807370935 1107 42-43 1.1860545552986181 1107 44-45 1.1121658117728899 1107 46-47 0.9824675887363838 1107 48-49 0.9736826996544892 1107 50-51 1.3179816499420163 1107 52-53 1.4020418646774573 1107 54-55 1.5640619175074022 1107 56-57 1.7311587791759884 1107 58-59 1.8929029936497486 1107 60-61 1.920845801824992 1107 62-63 1.835086047707989 1107 64-65 2.0811180207716937 1107 66-67 2.238340142123903 1107 68-69 2.3552161527517974 1107 70-71 2.356150692739039 1107 72-73 2.424639106339974 1107 74-75 2.633168319062282 1107 76-77 2.6364198429793397 1107 78-79 2.852889847715243 1107 80-81 2.8719809805244196 1107 82-83 3.0036645072250856 1107 84-85 3.0619581699155347 1107 86-87 3.210525410424367 1107 88-89 3.3224242458547657 1107 90-91 3.113721607015279 1107 92-93 2.912532354984405 1107 94-95 3.014768104750253 1107 96-97 2.707308189567458 1107 98-99 2.4492263894232558 1107 100-101 1.553060240075177 1108 1 0.2220784584401514 1108 2 0.20128718349982222 1108 3 0.08447689048022156 1108 4 3.823697376645896E-4 1108 5 0.10157340534821202 1108 6 0.1447820307639418 1108 7 0.010135155809344099 1108 8 0.06671035463183017 1108 9 0.18315788578426861 1108 10-11 0.12463187047352875 1108 12-13 0.1477871473080583 1108 14-15 0.21517891742031026 1108 16-17 0.27537013511886954 1108 18-19 0.25824204106005766 1108 20-21 0.2090347070920089 1108 22-23 0.22361538866093866 1108 24-25 0.10780719919098658 1108 26-27 0.1588006676008149 1108 28-29 0.17360718910202166 1108 30-31 0.3680045704745183 1108 32-33 0.32094741091717793 1108 34-35 0.4506584959206279 1108 36-37 0.5191398625067052 1108 38-39 0.2510213746066512 1108 40-41 0.44312573779478015 1108 42-43 0.628798767981884 1108 44-45 0.4398886561567181 1108 46-47 0.64755277582546 1108 48-49 0.6668537108382679 1108 50-51 0.7430735475803694 1108 52-53 0.7572361088326858 1108 54-55 1.1580581985673462 1108 56-57 1.1699064554295795 1108 58-59 1.2348151738878244 1108 60-61 1.2095872829978553 1108 62-63 1.3652519529156883 1108 64-65 1.3924509751489431 1108 66-67 1.438564560105526 1108 68-69 1.7183471938422024 1108 70-71 1.6807930214084017 1108 72-73 1.856526321821434 1108 74-75 1.866997266735602 1108 76-77 1.7248394873438748 1108 78-79 2.0032697574940634 1108 80-81 2.0781078680732996 1108 82-83 2.462260914740334 1108 84-85 2.329493440496325 1108 86-87 2.310132850385287 1108 88-89 2.3457580647058727 1108 90-91 2.390113242048411 1108 92-93 2.4135534792638733 1108 94-95 2.322141054757843 1108 96-97 2.0993853906772753 1108 98-99 1.8160898484252979 1108 100-101 1.2988756049157777 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>>Per sequence quality scores pass #Quality Count 2 6018.0 3 2297.0 4 2470.0 5 2898.0 6 3652.0 7 4612.0 8 5776.0 9 7588.0 10 9760.0 11 12150.0 12 15244.0 13 18334.0 14 21123.0 15 24245.0 16 28564.0 17 33507.0 18 32877.0 19 31302.0 20 34145.0 21 37520.0 22 41543.0 23 46244.0 24 51469.0 25 56437.0 26 62875.0 27 69874.0 28 76776.0 29 84773.0 30 93665.0 31 104700.0 32 117467.0 33 132139.0 34 149888.0 35 165031.0 36 164777.0 37 119050.0 38 30406.0 39 419.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97746697172444 18.895773922206043 14.857418573541691 22.269340532527824 2 15.306010802485371 20.286116470739234 40.6900103311654 23.71786239560999 3 19.44656475498533 25.35305143227489 29.212484224801898 25.987899587937886 4 12.766334666804152 16.749280830637982 37.022070660625715 33.46231384193215 5 13.722415569463461 37.864271289039685 33.44802619185682 14.96528694964003 6 31.37770322814188 36.021083890438696 17.821496181728065 14.779716699691361 7 28.739110924296362 32.714547473713395 20.499475767186972 18.04686583480327 8 26.612778068585648 34.787238240036615 19.468444131131847 19.13153956024589 9 27.051555806663224 16.99037987575431 20.01356191846962 35.94450239911285 10-11 25.173017593596263 26.91769988855223 26.815773975407843 21.093508542443658 12-13 26.449587169862138 24.274049144040262 27.395419521268536 21.88094416482906 14-15 23.204289983664545 25.533729652021393 26.223364146528326 25.038616217785737 16-17 22.04676676946253 28.792764910615993 26.772124034612204 22.388344285309277 18-19 22.1049807743897 27.236298793862534 29.308430338065655 21.350290093682112 20-21 23.18381739093383 26.119094424504425 29.280675904362962 21.41641228019878 22-23 22.667550414388256 26.102961869968095 29.21693639383586 22.01255132180779 24-25 22.364553995982302 26.355841008227678 29.39696939327629 21.88263560251373 26-27 22.08333771712942 26.45096636400939 29.440513640619905 22.02518227824129 28-29 21.895072172697912 26.85017415554353 29.468675015997903 21.78607865576065 30-31 22.093677751271052 26.40320675860146 29.368669400337193 22.134446089790295 32-33 21.98185815442793 26.495042671920537 29.205089016763942 22.318010156887592 34-35 22.540075889659896 26.522639205344205 29.04732359678167 21.889961308214225 36-37 22.51690744637789 26.227563589173446 29.13601522137031 22.119513743078357 38-39 22.136012814299168 26.557272117407255 29.158421270348107 22.148293797945474 40-41 22.319302035152273 26.49910734783298 29.074847352403406 22.106743264611346 42-43 22.13359209718371 26.44598107394887 28.867658331788377 22.552768497079047 44-45 22.179238810558335 26.634004416267242 28.73511048162663 22.451646291547796 46-47 22.510764903141514 26.90440755490673 28.287979995807916 22.296847546143848 48-49 22.4276709348045 26.742076366785472 28.282003735946482 22.548248962463546 50-51 22.450848264355017 27.004771528085485 28.140418879420633 22.403961328138866 52-53 22.52303991446431 27.20562461328417 27.87054575020833 22.40078972204319 54-55 22.21220063314822 27.025710109203473 28.12813165528106 22.633957602367246 56-57 22.36303973338053 27.075345403330587 28.00577352964456 22.555841333644327 58-59 22.51414189036574 26.685647858055468 28.029564590436706 22.770645661142087 60-61 22.37564121307996 26.818983588521327 28.403938207937628 22.401436990461086 62-63 22.257708153474884 26.904413348308427 28.31220700549259 22.525671492724097 64-65 22.039532243767358 26.89545746545879 28.243395011361006 22.821615279412853 66-67 22.117339258363298 26.910460614108324 27.95969051359755 23.01250961393083 68-69 22.177348103489262 26.76235293127712 28.145319570106004 22.914979395127617 70-71 22.239774097114257 26.865650054592848 27.915063418164376 22.97951243012852 72-73 22.43195668031802 26.737575858617785 27.827930907715846 23.002536553348353 74-75 22.21102572908542 27.195750054302408 27.61880919685556 22.97441501975661 76-77 22.38258955746837 26.820658926296442 27.686579428636037 23.11017208759915 78-79 22.489129245747417 26.813764573226916 27.67190727830393 23.025198902721737 80-81 22.452996006586794 27.100170875800274 27.48252454400249 22.964308573610445 82-83 22.541033290775907 26.9742985585261 27.44378015123445 23.04088799946354 84-85 22.55332979392268 26.741798940964156 27.48089415084779 23.223977114265377 86-87 22.5367611387769 26.813892651137888 27.359784682786536 23.289561527298677 88-89 22.557387723501744 26.797566611677958 27.204682902404382 23.440362762415916 90-91 22.617129280301544 26.756863591856767 27.235577245505517 23.39042988233617 92-93 22.78219746624007 26.53605612906466 27.229912009733866 23.451834394961406 94-95 22.636589042580162 26.413360095181286 27.18810191803763 23.761948944200917 96-97 22.599347588486335 26.385769602588603 27.14453197731602 23.870350831609034 98-99 22.681204791333084 26.383361869451928 26.683063430124726 24.252369909090262 100-101 23.68619739221772 25.464549061108873 26.834937859175547 24.014315687497863 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 925.0 1 679.0 2 625.0 3 764.5 4 622.5 5 548.5 6 818.5 7 1775.5 8 2794.5 9 3144.5 10 2762.0 11 2219.0 12 2136.0 13 2281.0 14 2466.5 15 2574.0 16 2735.0 17 2939.5 18 3052.5 19 3235.5 20 3622.0 21 4103.0 22 4645.0 23 5386.5 24 6298.5 25 7410.0 26 9013.5 27 11281.0 28 13721.5 29 16536.5 30 19918.5 31 23301.0 32 27177.0 33 30981.0 34 34479.0 35 37999.5 36 42187.5 37 46360.5 38 49309.0 39 51627.5 40 54312.5 41 57655.0 42 60825.5 43 64004.5 44 66499.5 45 69036.5 46 71471.5 47 73924.5 48 77472.0 49 77281.0 50 73202.0 51 69528.5 52 66715.0 53 64451.5 54 62437.0 55 58869.5 56 53870.5 57 49080.0 58 44811.5 59 39980.0 60 33991.5 61 28341.5 62 23125.0 63 18100.0 64 13830.0 65 10635.0 66 8097.5 67 6229.5 68 4739.0 69 3476.0 70 2690.5 71 2089.5 72 1680.5 73 1407.5 74 1099.5 75 869.0 76 629.0 77 395.5 78 265.5 79 187.5 80 125.5 81 73.5 82 41.5 83 26.5 84 17.0 85 9.5 86 6.0 87 4.0 88 2.0 89 1.5 90 2.0 91 1.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38540924424765266 2 0.030027108536691178 3 0.036179773508307415 4 0.04264795976051935 5 0.061894757876857304 6 0.05348085706097185 7 0.03901946503366875 8 0.04101776647744154 9 0.03702116358989596 10-11 0.03809919463193128 12-13 0.031078846138676862 14-15 0.0281602742931666 16-17 0.022927879723287838 18-19 0.026556374450138434 20-21 0.03344525574314464 22-23 0.0326038656615561 24-25 0.04072853863689548 26-27 0.03549614406701672 28-29 0.030737031418031517 30-31 0.03312973446254894 32-33 0.028423208693663018 34-35 0.029737880696145115 36-37 0.03173618213991791 38-39 0.015854944349934137 40-41 0.013593708505664921 42-43 0.014066990426558477 44-45 0.01238421026338139 46-47 0.021744674921053947 48-49 0.018747222755394758 50-51 0.012226449623083536 52-53 0.009807453138516472 54-55 0.008650541776332222 56-57 0.009386758097722199 58-59 0.004391004488290216 60-61 0.007414750093999048 62-63 0.01238421026338139 64-65 0.008124672975339382 66-67 0.004075483207694512 68-69 0.006468186252211936 70-71 0.014040696986508837 72-73 0.011358766101445351 74-75 0.011884634902438192 76-77 0.005916024011169453 78-79 0.009623399058168978 80-81 0.012305329943232465 82-83 0.013804056026062059 84-85 0.008860889296729358 86-87 0.0025504636848152754 88-89 0.01659116067132411 90-91 0.025162822127507412 92-93 0.011122125140998572 94-95 0.021087338919812897 96-97 0.018983863715841535 98-99 0.01175316770218998 100-101 0.12094982422835326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1901615.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.215213688547706 #Duplication Level Percentage of deduplicated Percentage of total 1 78.62160909103261 44.98352164680895 2 12.161145820107357 13.916051135900624 3 3.838351260251835 6.588362627010453 4 1.703532862218289 3.8987198694913086 5 0.9234959424666856 2.641900884436909 6 0.5809373860811016 1.994307401057794 7 0.37362697011165075 1.4964002854319907 8 0.2868326218307891 1.3128951800715993 9 0.216595431296007 1.1153298497007775 >10 1.1665086402233233 12.170176937779996 >50 0.08605801121400806 3.4386404195555027 >100 0.03770299065679128 3.8221656637135664 >500 0.002679133404550852 1.008523996485477 >1k 7.390712840140279E-4 0.8115235281992415 >5k 1.8476782100350697E-4 0.8014805743558326 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8424 0.4429918779563687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6739 0.35438298498907506 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4796 0.2522066769561662 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2617 0.1376198652198263 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.6293440049642013E-5 0.0 14-15 0.0 0.0 0.0 1.0517376019856807E-4 0.0 16-17 0.0 0.0 0.0 2.103475203971361E-4 0.0 18-19 0.0 0.0 0.0 2.103475203971361E-4 0.0 20-21 0.0 0.0 0.0 2.8922784054606214E-4 0.0 22-23 0.0 0.0 0.0 6.836294412906924E-4 0.0 24-25 0.0 0.0 0.0 0.0011306179221346065 0.0 26-27 0.0 0.0 0.0 0.002392703044517423 0.0 28-29 0.0 0.0 0.0 0.005495328970375181 0.0 30-31 2.6293440049642013E-5 0.0 0.0 0.014198457626806688 0.0 32-33 5.2586880099284027E-5 0.0 0.0 0.03257757222150646 0.0 34-35 5.2586880099284027E-5 0.0 0.0 0.058713251630850616 0.0 36-37 5.2586880099284027E-5 0.0 0.0 0.10575221587966019 0.0 38-39 5.2586880099284027E-5 0.0 0.0 0.17479878945002011 0.0 40-41 5.2586880099284027E-5 0.0 0.0 0.25094459183378337 0.0 42-43 5.2586880099284027E-5 0.0 0.0 0.3284576531001281 0.0 44-45 5.2586880099284027E-5 0.0 0.0 0.4241394814407753 0.0 46-47 5.2586880099284027E-5 0.0 0.0 0.5377534358952785 0.0 48-49 5.2586880099284027E-5 0.0 0.0 0.6598075846057168 0.0 50-51 5.2586880099284027E-5 0.0 0.0 0.7896971784509483 0.0 52-53 5.2586880099284027E-5 0.0 0.0 0.9208488574185627 0.0 54-55 5.2586880099284027E-5 0.0 0.0 1.0598885684010697 0.0 56-57 5.2586880099284027E-5 0.0 0.0 1.2298493648819555 0.0 58-59 1.0517376019856805E-4 0.0 0.0 1.4170060711553076 0.0 60-61 1.0517376019856805E-4 0.0 0.0 1.6256445179492167 0.0 62-63 1.0517376019856805E-4 0.0 0.0 1.828340647291907 0.0 64-65 1.0517376019856805E-4 0.0 0.0 2.039240329930086 0.0 66-67 1.0517376019856805E-4 0.0 0.0 2.275749823176616 0.0 68-69 1.0517376019856805E-4 0.0 0.0 2.5409191660772557 0.0 70-71 1.0517376019856805E-4 0.0 0.0 2.8284116395800414 0.0 72-73 1.0517376019856805E-4 5.2586880099284027E-5 0.0 3.1181390554870463 0.0 74-75 1.0517376019856805E-4 5.2586880099284027E-5 0.0 3.418489021174107 0.0 76-77 1.0517376019856805E-4 5.2586880099284027E-5 0.0 3.735719375373038 0.0 78-79 1.0517376019856805E-4 5.2586880099284027E-5 0.0 4.087341549156901 0.0 80-81 1.0517376019856805E-4 5.2586880099284027E-5 0.0 4.470410677240135 0.0 82-83 1.0517376019856805E-4 5.2586880099284027E-5 0.0 4.8551888789265965 0.0 84-85 1.0517376019856805E-4 5.2586880099284027E-5 0.0 5.236575226846654 0.0 86-87 1.0517376019856805E-4 5.2586880099284027E-5 0.0 5.621774123573909 0.0 88-89 1.0517376019856805E-4 5.2586880099284027E-5 0.0 6.03894584340153 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8475 0.0 29.151604 1 GTATCAA 12060 0.0 28.553694 1 GTACATG 10530 0.0 25.591307 1 TACATGG 10520 0.0 25.075499 2 ACATGGG 10835 0.0 23.513008 3 TATCAAC 14615 0.0 23.44816 2 ATCAACG 14605 0.0 23.33404 3 TCAACGC 14775 0.0 23.00122 4 CAACGCA 14850 0.0 22.788431 5 TACCTGG 1665 0.0 22.552006 2 AACGCAG 15275 0.0 22.211353 6 GAGTACT 7390 0.0 22.207895 12-13 GGACCGA 500 0.0 20.907913 6 GTACTTT 7915 0.0 20.526691 14-15 AGTACTT 7760 0.0 20.41446 12-13 CATGGGG 4715 0.0 20.060593 4 ACGATAC 265 1.843E-8 19.729637 3 ACGCAGA 17035 0.0 19.687178 7 CGCAGAG 17050 0.0 19.614136 8 CATGGGA 6060 0.0 19.37299 4 >>END_MODULE