FastQCFastQC Report
Thu 26 May 2016
SRR938392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901615
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82350.433052957617604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70630.3714211341412431No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50680.26651030834317146No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26680.1403017961048898No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85950.029.852641
GTATCAA122650.028.4744051
GTACATG106300.024.5399671
TACATGG106500.023.997432
GGACCGA5000.023.7483586
ATCAACG149900.023.0055583
TATCAAC150950.022.9732172
TCAACGC151250.022.8298264
CAACGCA152750.022.6367325
ACATGGG108950.022.3223823
AACGCAG156950.022.030976
GAGTACT77200.022.02567912-13
GTACTTT80200.020.96488214-15
TATCACG1604.988087E-520.783092
AATACCG1150.00372565520.6507435
AGTACTT80750.020.35153212-13
CATGGGA60450.019.878694
ACGCAGA174050.019.839197
CGCAGAG174350.019.6960858
CATGGGG45150.019.5667534
Produced by FastQC (version 0.11.5)