##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938392_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1901615 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81308887445671 31.0 31.0 34.0 30.0 34.0 2 32.06505891045243 33.0 31.0 34.0 30.0 34.0 3 32.11603084746387 33.0 31.0 34.0 30.0 34.0 4 35.76604465151989 37.0 35.0 37.0 35.0 37.0 5 35.665637366133524 37.0 35.0 37.0 33.0 37.0 6 35.70707056896375 37.0 35.0 37.0 33.0 37.0 7 35.632829989245984 37.0 35.0 37.0 33.0 37.0 8 35.60897289935134 37.0 35.0 37.0 33.0 37.0 9 37.14606268881977 39.0 37.0 39.0 33.0 39.0 10-11 37.07625597189757 39.0 37.0 39.0 33.0 39.0 12-13 36.954449770326804 39.0 37.0 39.0 33.0 39.0 14-15 37.936854463179984 40.0 37.0 41.0 33.0 41.0 16-17 38.039707301425366 40.0 37.0 41.0 33.0 41.0 18-19 38.19622978363128 40.0 37.5 41.0 33.0 41.0 20-21 38.161233214925204 40.0 37.0 41.0 33.0 41.0 22-23 38.028541266239486 40.0 37.0 41.0 33.0 41.0 24-25 37.9047138879321 40.0 37.0 41.0 32.5 41.0 26-27 37.68159117381805 40.0 37.0 41.0 32.0 41.0 28-29 37.53606171596248 39.5 36.5 41.0 32.0 41.0 30-31 37.276049568393184 39.0 36.0 41.0 31.0 41.0 32-33 37.153616268277226 39.0 36.0 41.0 31.0 41.0 34-35 37.00591339466716 39.0 36.0 41.0 31.0 41.0 36-37 36.87348779852914 39.0 35.0 41.0 30.5 41.0 38-39 36.65921124938539 39.0 35.0 40.0 30.0 41.0 40-41 36.434219071683806 39.0 35.0 40.0 30.0 41.0 42-43 36.15824259905396 38.5 35.0 40.0 30.0 41.0 44-45 35.83566074100173 38.0 35.0 40.0 29.0 41.0 46-47 35.37465943421776 38.0 34.0 40.0 27.0 41.0 48-49 34.9357525051075 38.0 34.0 40.0 26.0 41.0 50-51 34.53033342711327 38.0 33.0 40.0 24.0 41.0 52-53 33.93268668999771 37.5 33.0 40.0 23.5 41.0 54-55 33.50300323672247 37.0 32.0 40.0 22.5 41.0 56-57 33.10627992522146 37.0 32.0 39.0 21.0 40.5 58-59 32.56286472288029 36.0 31.0 39.0 18.5 40.0 60-61 32.0494177317701 36.0 31.0 39.0 17.5 40.0 62-63 31.523916250134754 35.0 30.0 38.5 12.5 40.0 64-65 30.85303597205533 35.0 29.0 38.0 8.0 40.0 66-67 30.188266026509048 34.0 28.0 38.0 7.0 40.0 68-69 29.444198220985847 34.0 27.5 37.0 2.0 39.0 70-71 28.646283553716184 33.5 26.0 36.0 2.0 39.0 72-73 28.051544608135714 33.0 26.0 36.0 2.0 38.5 74-75 27.32301438514105 32.0 25.0 35.0 2.0 38.0 76-77 26.16767011198376 30.5 23.0 34.0 2.0 36.0 78-79 26.65624298293819 32.0 24.5 35.0 2.0 36.0 80-81 26.249388283117245 32.0 23.5 35.0 2.0 36.0 82-83 25.55445871009642 31.0 21.0 34.5 2.0 36.0 84-85 24.756556137809177 31.0 18.5 34.0 2.0 35.0 86-87 23.83106622528745 30.5 10.0 34.0 2.0 35.0 88-89 22.845664869071815 29.5 4.5 34.0 2.0 35.0 90-91 21.959262258659088 29.0 2.0 34.0 2.0 35.0 92-93 20.85703388961488 28.0 2.0 33.5 2.0 35.0 94-95 19.67257541615942 26.5 2.0 33.0 2.0 34.0 96-97 18.29220872784449 24.5 2.0 32.0 2.0 34.0 98-99 16.534135458544448 11.0 2.0 32.0 2.0 34.0 100-101 12.352138839880837 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2334449732938566 1101 2 0.3278598564926227 1101 3 0.2376767467356231 1101 4 0.10439482019471313 1101 5 0.06652007733241305 1101 6 0.11581265691060594 1101 7 0.16544321482335533 1101 8 0.23044187912331893 1101 9 0.2186242264487248 1101 10-11 0.21710355439580553 1101 12-13 0.3441472574654014 1101 14-15 0.4708587043410617 1101 16-17 0.3738567911145978 1101 18-19 0.3094641519368153 1101 20-21 0.40501984841574057 1101 22-23 0.3568765259482021 1101 24-25 0.41509919864932954 1101 26-27 0.3566539344673245 1101 28-29 0.49733951218927075 1101 30-31 0.5480384856561642 1101 32-33 0.46528325018815764 1101 34-35 0.38069874165353923 1101 36-37 0.41669342141411647 1101 38-39 0.5151056195696455 1101 40-41 0.589859340728232 1101 42-43 0.5675617609137973 1101 44-45 0.45628915957643557 1101 46-47 0.5529007201594709 1101 48-49 0.5622268691290273 1101 50-51 0.6103394498272863 1101 52-53 0.7192036179568433 1101 54-55 0.5360512570215903 1101 56-57 0.6944871441506066 1101 58-59 0.9189268214203068 1101 60-61 0.8810748922566987 1101 62-63 1.057458533823823 1101 64-65 1.3025207887277617 1101 66-67 1.2350282156771932 1101 68-69 1.2284541032194198 1101 70-71 1.5520379739046746 1101 72-73 1.7441189297408926 1101 74-75 1.5857529998974798 1101 76-77 1.8000018902026333 1101 78-79 2.0387302906833646 1101 80-81 2.1081350593725467 1101 82-83 2.2792238950410386 1101 84-85 2.480720414229147 1101 86-87 2.549102691455758 1101 88-89 2.3795926778017034 1101 90-91 2.5933344951682713 1101 92-93 2.9253470102599195 1101 94-95 2.8545860836260175 1101 96-97 2.8219745777747285 1101 98-99 2.759323359946677 1101 100-101 2.310222657703246 1102 1 0.27877686751943287 1102 2 0.22748209070741154 1102 3 0.24739072406967466 1102 4 0.21124857086929438 1102 5 0.15556486956123194 1102 6 0.19514347180537328 1102 7 0.26042432653409975 1102 8 0.20183961252860882 1102 9 0.3270969733456539 1102 10-11 0.32827462832997156 1102 12-13 0.33389361472390533 1102 14-15 0.5930929191278906 1102 16-17 0.3800629433002385 1102 18-19 0.49699788102477527 1102 20-21 0.4198606471205366 1102 22-23 0.4599526187706502 1102 24-25 0.4458601268738249 1102 26-27 0.5509334811483839 1102 28-29 0.477371892113716 1102 30-31 0.6311469584030576 1102 32-33 0.537328582082381 1102 34-35 0.6292146617830596 1102 36-37 0.6298612573558344 1102 38-39 0.6111660620952719 1102 40-41 0.5129570201669864 1102 42-43 0.548943304356861 1102 44-45 0.6942816042607376 1102 46-47 0.7123718480601724 1102 48-49 0.8463607061500795 1102 50-51 0.9576141395196771 1102 52-53 0.9234669746756836 1102 54-55 0.9381019855698796 1102 56-57 1.1540122385920526 1102 58-59 1.3296121964877656 1102 60-61 1.1587327443883737 1102 62-63 1.2066760189830212 1102 64-65 1.5753216521924145 1102 66-67 1.602000692741413 1102 68-69 1.544698031983586 1102 70-71 1.7422700300298786 1102 72-73 1.8768766199729505 1102 74-75 1.8094443112844232 1102 76-77 1.6318961157827765 1102 78-79 1.8298797780012244 1102 80-81 2.1278328467443792 1102 82-83 2.1362125620674846 1102 84-85 2.364152686220514 1102 86-87 2.019421094046155 1102 88-89 2.0269213340348387 1102 90-91 2.2773603991078346 1102 92-93 2.5912941230499875 1102 94-95 2.527279014009178 1102 96-97 2.605838582092055 1102 98-99 2.7989887673940608 1102 100-101 2.220638200066981 1103 1 0.28017624239515015 1103 2 0.2958170451231794 1103 3 0.19549010329799188 1103 4 0.15356269141239665 1103 5 0.27347194640395145 1103 6 0.23523859174508033 1103 7 0.2676595981775378 1103 8 0.24317109823572025 1103 9 0.342866184310445 1103 10-11 0.36510672126735244 1103 12-13 0.4144093038527359 1103 14-15 0.4556102406236775 1103 16-17 0.40393054918006754 1103 18-19 0.43239193776390294 1103 20-21 0.4208079775405693 1103 22-23 0.3975168587257514 1103 24-25 0.42701820844153815 1103 26-27 0.5131384111924433 1103 28-29 0.4534826654795623 1103 30-31 0.440187762171675 1103 32-33 0.7084440508502112 1103 34-35 0.677896777237315 1103 36-37 0.7862239750960356 1103 38-39 0.6485925835349562 1103 40-41 0.8592459741520386 1103 42-43 0.7823262611144983 1103 44-45 0.8743110824523654 1103 46-47 1.0208037146623923 1103 48-49 1.136611794228763 1103 50-51 1.1177663211818043 1103 52-53 1.1548709212030062 1103 54-55 1.1454435971866062 1103 56-57 1.3617312177169296 1103 58-59 1.5921248524523506 1103 60-61 1.6413121060697478 1103 62-63 1.6247069030926475 1103 64-65 1.8120764509330627 1103 66-67 1.6534599371927747 1103 68-69 1.7799375287775945 1103 70-71 1.9707065280252607 1103 72-73 2.029877452656475 1103 74-75 1.8274767316055431 1103 76-77 1.9421294748429112 1103 78-79 1.8622368015174935 1103 80-81 2.067234281850393 1103 82-83 2.4862134581908535 1103 84-85 2.4435596004808495 1103 86-87 2.517285902314157 1103 88-89 2.1420891182005377 1103 90-91 2.2340656920107342 1103 92-93 2.671554772622791 1103 94-95 1.969277642229212 1103 96-97 2.0682516118965495 1103 98-99 1.890222660998898 1103 100-101 1.570641656542838 1104 1 0.19742721213987124 1104 2 0.2559597341076696 1104 3 0.2461624533394584 1104 4 0.1561001822250958 1104 5 0.08973342699110987 1104 6 0.08824550891102945 1104 7 0.10975322648709351 1104 8 0.1112631432114739 1104 9 0.2462355866680639 1104 10-11 0.23114024335372108 1104 12-13 0.2759620668457998 1104 14-15 0.33830466664627323 1104 16-17 0.43559155703438535 1104 18-19 0.26390953865419675 1104 20-21 0.2639200031308775 1104 22-23 0.39038524363079574 1104 24-25 0.3714720999850627 1104 26-27 0.4268618341409578 1104 28-29 0.3557665961529679 1104 30-31 0.44740912470788174 1104 32-33 0.5226961334631923 1104 34-35 0.424157143066779 1104 36-37 0.5713034157521903 1104 38-39 0.6278514775063826 1104 40-41 0.5987110416137753 1104 42-43 0.8246350943410832 1104 44-45 0.8195386914104787 1104 46-47 0.8565035463150892 1104 48-49 0.7612076945656483 1104 50-51 0.8919211364626776 1104 52-53 0.7944036298941484 1104 54-55 0.901127444347658 1104 56-57 1.0541459529241948 1104 58-59 1.216111414898613 1104 60-61 1.1622856107356654 1104 62-63 1.0615898894340354 1104 64-65 1.2125276443850908 1104 66-67 1.1986331308601947 1104 68-69 1.406739326749495 1104 70-71 1.506483636948424 1104 72-73 1.5654240523621041 1104 74-75 1.751942450917113 1104 76-77 1.873497244741806 1104 78-79 1.8415183685267102 1104 80-81 2.1757597219435034 1104 82-83 2.173883860240494 1104 84-85 2.485847650089454 1104 86-87 2.1850049758182593 1104 88-89 2.4326762176199175 1104 90-91 2.801297862028285 1104 92-93 2.8743254178407653 1104 94-95 2.542141228007928 1104 96-97 2.971473639508531 1104 98-99 3.2283872408013785 1104 100-101 2.901125744793859 1105 1 0.07289328744620249 1105 2 0.14497436727461377 1105 3 0.118475866036583 1105 4 0.05340721068340315 1105 5 0.08631944396865521 1105 6 -0.0011033039051611127 1105 7 0.0570547309940963 1105 8 0.0800969323031282 1105 9 0.15509996318472474 1105 10-11 0.2040116915629966 1105 12-13 0.10626511831789998 1105 14-15 0.25824307782333733 1105 16-17 0.15741701207472403 1105 18-19 0.19438588954489688 1105 20-21 0.27229338187704144 1105 22-23 0.28975049375971196 1105 24-25 0.15425700974026313 1105 26-27 0.1898253318189589 1105 28-29 0.2915336504794155 1105 30-31 0.2977319071648381 1105 32-33 0.42538455130263486 1105 34-35 0.3487136810318461 1105 36-37 0.3827223157381425 1105 38-39 0.4465370254784702 1105 40-41 0.46439804133719775 1105 42-43 0.3733227095904965 1105 44-45 0.5496379129602573 1105 46-47 0.5195403172020292 1105 48-49 0.5629231983952891 1105 50-51 0.5616484861277584 1105 52-53 0.48141215023328954 1105 54-55 0.5149912167225708 1105 56-57 0.6644675400343161 1105 58-59 0.8292481473119508 1105 60-61 0.742155090244907 1105 62-63 0.9163500736209436 1105 64-65 0.9848788838588547 1105 66-67 1.0156495917176294 1105 68-69 1.052133088746622 1105 70-71 1.1803051789096273 1105 72-73 1.3727602736741034 1105 74-75 1.3475850753295617 1105 76-77 1.3685698025537754 1105 78-79 1.4578029769872316 1105 80-81 1.6492464374227573 1105 82-83 1.8613367546486792 1105 84-85 1.9567269829306788 1105 86-87 2.112450656748102 1105 88-89 2.120995867854191 1105 90-91 2.185321404668919 1105 92-93 2.36662901420474 1105 94-95 1.8149862595694835 1105 96-97 2.1312288987112105 1105 98-99 2.195388552836839 1105 100-101 2.0771718737976563 1106 1 0.00412066205912609 1106 2 -0.029682867018799186 1106 3 -0.030187146881104354 1106 4 0.05599541244290407 1106 5 0.004748591038534755 1106 6 -0.10026605121343124 1106 7 -0.011669436871052596 1106 8 -0.06506755118373775 1106 9 -0.016741293083008202 1106 10-11 -0.017606064102260177 1106 12-13 -0.031099258925202378 1106 14-15 -0.0038190877355432917 1106 16-17 -0.04187348688227388 1106 18-19 -0.08867898393432938 1106 20-21 0.04433987000235362 1106 22-23 0.09822485921234403 1106 24-25 0.0019081155823670315 1106 26-27 -0.1055191182399966 1106 28-29 0.14049738507731035 1106 30-31 0.06825988684896345 1106 32-33 -0.16959698560410885 1106 34-35 -0.2597894479765799 1106 36-37 -0.13261954300947565 1106 38-39 0.12036555840756336 1106 40-41 0.14568177697160678 1106 42-43 0.27897319351789207 1106 44-45 0.2818345675773273 1106 46-47 -0.20542839271362823 1106 48-49 -0.08283145624769617 1106 50-51 0.34411696930367697 1106 52-53 0.16580856904102603 1106 54-55 0.11406611857665183 1106 56-57 0.0323416282166491 1106 58-59 0.2975573166671026 1106 60-61 0.2054279164586177 1106 62-63 0.14481057555795118 1106 64-65 0.38136493612560685 1106 66-67 0.3425655425345404 1106 68-69 0.4213901597874212 1106 70-71 0.33338741417478346 1106 72-73 0.6953618206869656 1106 74-75 0.62156118343907 1106 76-77 0.6510071899936882 1106 78-79 0.9658993996819341 1106 80-81 1.0146139501525724 1106 82-83 1.4073176307710398 1106 84-85 1.3067527664784464 1106 86-87 1.3763512281322505 1106 88-89 1.5733654925027416 1106 90-91 1.4657564858417516 1106 92-93 1.4210747974933717 1106 94-95 1.3124098482765483 1106 96-97 1.2510502277947921 1106 98-99 1.5120640299620405 1106 100-101 1.4812009742239258 1107 1 0.08152955578201215 1107 2 0.0513930309171684 1107 3 0.08354399115037126 1107 4 0.08426880003369774 1107 5 0.07780463624474976 1107 6 0.048030839609054965 1107 7 0.08015318873997046 1107 8 0.15884898734030628 1107 9 0.14431485213695794 1107 10-11 0.09298314191356383 1107 12-13 0.20655191811164286 1107 14-15 0.3531509956509211 1107 16-17 0.2402510552422683 1107 18-19 0.2078664160581738 1107 20-21 0.2568118348975261 1107 22-23 0.24652850096308754 1107 24-25 0.35183573062943907 1107 26-27 0.32024717462527263 1107 28-29 0.3040757521027757 1107 30-31 0.3267231755511588 1107 32-33 0.2990190741040948 1107 34-35 0.29977774251210576 1107 36-37 0.3452636659317534 1107 38-39 0.42210779796550213 1107 40-41 0.49874232279665875 1107 42-43 0.46107085694661265 1107 44-45 0.5810790886989778 1107 46-47 0.6188460700250005 1107 48-49 0.6771644181850363 1107 50-51 0.5562923934244921 1107 52-53 0.5186058286642208 1107 54-55 0.5863085519112374 1107 56-57 0.6293623435650062 1107 58-59 0.7896020978245062 1107 60-61 0.9860309993825211 1107 62-63 0.9973979313193659 1107 64-65 1.1008249868825857 1107 66-67 1.0759313803372414 1107 68-69 1.2480729536715813 1107 70-71 1.1678176483319795 1107 72-73 1.4484717483020155 1107 74-75 1.3163598432547943 1107 76-77 1.35193856805817 1107 78-79 1.7091902271707156 1107 80-81 1.66535130153839 1107 82-83 1.8992708993454706 1107 84-85 2.2072083291183056 1107 86-87 2.327087079311788 1107 88-89 2.242976814059457 1107 90-91 2.3099858173748373 1107 92-93 2.3316134965644935 1107 94-95 2.2600295209815435 1107 96-97 2.0465449833236775 1107 98-99 1.9688172085240865 1107 100-101 1.6897042684268797 1108 1 -0.14054446266419518 1108 2 -0.09574817578051764 1108 3 -0.05045773306827783 1108 4 -0.03477133132092547 1108 5 -0.020268693648979763 1108 6 -0.010342899680196638 1108 7 -0.010940744289790416 1108 8 -0.04310331432134262 1108 9 -0.07938100061117126 1108 10-11 -0.0032555455616360973 1108 12-13 -0.05625830292032674 1108 14-15 0.15634604942280106 1108 16-17 -0.051525306126727344 1108 18-19 -0.10600794013816284 1108 20-21 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12 6253.0 13 8842.0 14 11695.0 15 15873.0 16 20436.0 17 25677.0 18 25824.0 19 23389.0 20 24210.0 21 26260.0 22 28850.0 23 32534.0 24 36391.0 25 40992.0 26 47054.0 27 54604.0 28 63883.0 29 75081.0 30 87472.0 31 100358.0 32 118109.0 33 139275.0 34 169310.0 35 202983.0 36 233151.0 37 205367.0 38 66118.0 39 1266.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.884344194907655 19.60806178520877 15.407483929607446 22.100110090276132 2 15.859583648456328 21.035367055613467 39.54450833975851 23.560540956171693 3 19.54255886426957 25.38391798811303 29.755445214448212 25.318077933169185 4 13.332772406612275 17.427554999303226 36.47199880101913 32.76767379306536 5 13.61173616658314 37.94712793218795 33.72607092204549 14.715064979183417 6 31.437804182234576 36.39406504471199 17.396423566284447 14.771707206768983 7 28.62782424412933 32.73969757285255 20.418959673750997 18.213518509267125 8 26.610132235038236 35.10328594551786 19.301603795512655 18.98497802393125 9 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29.18440491130812 22.281680564089985 34-35 22.49980278001294 26.557827529806517 29.049978174321435 21.892391515859117 36-37 22.51708042524369 26.284476375584248 29.168296879667142 22.03014631950492 38-39 22.118116193372845 26.6192833759385 29.193685512078503 22.06891491861015 40-41 22.331759132865272 26.51391338487637 29.062498274287446 22.09182920797091 42-43 22.1807058697315 26.509596369022464 28.855685156110106 22.454012605135926 44-45 22.21514048140412 26.646425859432355 28.775680951449406 22.36275270771412 46-47 22.48047645829373 26.948600781073136 28.234139989992713 22.336782770640422 48-49 22.37364030048143 26.834532752423595 28.27814778490914 22.513679162185827 50-51 22.39527980164229 27.005860807787062 28.258059596711217 22.340799793859432 52-53 22.616933501260768 27.158573107595384 27.825742855415005 22.39875053572884 54-55 22.225319005161403 27.0252653665437 28.06335667314362 22.686058955151278 56-57 22.318923651738128 27.134593490270113 27.9436426405976 22.602840217394164 58-59 22.55067403233567 26.753759304591096 27.953502680616214 22.74206398245702 60-61 22.341798944581317 26.96513226915017 28.272074000257675 22.42099478601084 62-63 22.204100199041342 27.035072819682217 28.181834914007304 22.578992067269137 64-65 21.99954880433148 26.947082846466213 28.091847241343405 22.961521107858903 66-67 22.057101688965783 27.050245647432504 27.853934105750007 23.038718557851713 68-69 22.070410947450124 26.87377717687823 28.043322613376624 23.012489262295016 70-71 22.153865085132946 26.989336426845828 27.799963609859834 23.056834878161396 72-73 22.25500437655345 26.914765142493692 27.70817157351624 23.122058907436617 74-75 22.03687392032562 27.309103051879585 27.455610099836193 23.198412927958604 76-77 21.99909024697428 26.952826939206936 27.8627640190049 23.18531879481388 78-79 22.13455405011004 26.99749949385128 27.785382424938803 23.08256403109988 80-81 22.011818916840316 27.23291417152548 27.704487638780247 23.050779272853962 82-83 22.078214041463735 27.09286099865402 27.704033914785498 23.124891045096746 84-85 22.02875700952765 26.963837858049278 27.65943963746878 23.3479654949543 86-87 21.92955861792964 26.963561347824406 27.638882936106736 23.467997098139218 88-89 21.874117526418335 27.022583435658635 27.415538897200538 23.687760140722492 90-91 21.864632861474192 26.94277398694725 27.611845130775986 23.580748020802574 92-93 21.922490273355713 26.8369402397394 27.575841794819272 23.664727692085613 94-95 21.677301933331876 26.674505623776863 27.575319590442536 24.072872852448725 96-97 21.60550374287119 26.630364190438126 27.674187466968863 24.089944599721814 98-99 21.575634394974795 26.717264009802193 27.25967138458626 24.447430210636746 100-101 21.67640225618869 26.30260399849812 27.54736239846783 24.473631346845366 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1027.0 1 727.5 2 602.5 3 784.0 4 633.0 5 529.5 6 873.5 7 1956.5 8 3012.5 9 3292.5 10 2888.5 11 2335.0 12 2256.5 13 2337.0 14 2468.0 15 2645.0 16 2802.0 17 2886.0 18 2993.5 19 3202.5 20 3460.0 21 3890.0 22 4573.0 23 5324.0 24 6170.0 25 7429.5 26 8964.0 27 11148.5 28 13480.0 29 16385.0 30 20209.5 31 23636.5 32 27386.5 33 31081.0 34 34500.5 35 37870.5 36 42398.5 37 46935.5 38 49942.5 39 52398.5 40 54666.0 41 58344.0 42 61900.0 43 64567.5 44 67429.0 45 70188.5 46 71976.5 47 74713.5 48 79611.5 49 79416.5 50 74249.5 51 69558.5 52 66466.5 53 64875.0 54 63111.0 55 59194.5 56 53322.5 57 48321.5 58 44174.5 59 39058.0 60 32622.5 61 26778.5 62 21778.0 63 16600.5 64 12430.0 65 9522.5 66 7066.0 67 5455.5 68 4188.5 69 2996.5 70 2307.5 71 1804.5 72 1454.5 73 1273.0 74 991.5 75 775.0 76 576.0 77 360.0 78 239.5 79 139.0 80 77.5 81 45.0 82 26.0 83 16.0 84 10.5 85 7.0 86 3.0 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023558922284479245 2 0.012673438103927452 3 0.001945714563673509 4 0.0 5 3.155212805957042E-4 6 0.0 7 0.0 8 5.2586880099284027E-5 9 2.6293440049642014E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016827801631770889 26-27 0.008597954896232938 28-29 2.6293440049642014E-4 30-31 0.0 32-33 1.840540803474941E-4 34-35 0.009728572818367545 36-37 0.0012094982422835327 38-39 0.012699731543977093 40-41 0.011910928342487833 42-43 0.004653938888786636 44-45 5.784556810921244E-4 46-47 2.6293440049642013E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.2586880099284027E-5 66-67 3.9440160074463026E-4 68-69 0.0011306179221346067 70-71 5.2586880099284027E-5 72-73 2.366409604467781E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3146720024821007E-4 82-83 0.0013409654425317428 84-85 0.005048340489531267 86-87 0.0040228963275952285 88-89 0.0 90-91 0.0012357916823331747 92-93 4.4698848084391427E-4 94-95 7.888032014892605E-5 96-97 0.0 98-99 0.0 100-101 5.2586880099284027E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1901615.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.6589106614138 #Duplication Level Percentage of deduplicated Percentage of total 1 76.77862993318784 41.96636274223866 2 13.040312525357262 14.255385546408359 3 4.223775047474012 6.926008289213724 4 1.8573356278081203 4.060797685944998 5 1.0405466921478246 2.8437574342568794 6 0.6515766822467667 2.1368682998391857 7 0.4242016164823911 1.623047878041683 8 0.32250961942844364 1.4102419580628691 9 0.23497674987092934 1.1559215860834051 >10 1.2798425928532282 12.858532836229255 >50 0.09707875838443539 3.6806194255687847 >100 0.04554813956725176 4.4197172342593145 >500 0.002701274351639919 1.0120264673895807 >1k 6.753185879099797E-4 0.5767791527879967 >5k 2.894222519614199E-4 1.0739334636752917 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8235 0.433052957617604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7063 0.3714211341412431 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5068 0.26651030834317146 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2668 0.1403017961048898 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.6293440049642013E-5 0.0 14-15 0.0 0.0 0.0 1.0517376019856807E-4 0.0 16-17 0.0 0.0 0.0 2.103475203971361E-4 0.0 18-19 0.0 0.0 0.0 2.103475203971361E-4 0.0 20-21 0.0 0.0 0.0 2.8922784054606214E-4 0.0 22-23 0.0 0.0 0.0 7.362163213899764E-4 0.0 24-25 0.0 0.0 0.0 0.0011832048022338906 0.0 26-27 0.0 0.0 0.0 0.0024978768047159913 0.0 28-29 0.0 0.0 0.0 0.005653089610673033 0.0 30-31 0.0 0.0 0.0 0.01422475106685633 0.0 32-33 0.0 0.0 0.0 0.03268274598170502 0.0 34-35 0.0 0.0 0.0 0.05908135979154561 0.0 36-37 0.0 0.0 0.0 0.10604144372020624 0.0 38-39 0.0 0.0 0.0 0.17532465825101295 0.0 40-41 0.0 0.0 0.0 0.25173339503527264 0.0 42-43 0.0 0.0 0.0 0.3295093907021137 0.0 44-45 0.0 0.0 0.0 0.4254015665631582 0.0 46-47 0.0 0.0 0.0 0.5395413898186541 0.0 48-49 0.0 0.0 0.0 0.661332604128596 0.0 50-51 0.0 0.0 0.0 0.7908015029330332 0.0 52-53 0.0 0.0 0.0 0.9218743015804987 0.0 54-55 0.0 0.0 0.0 1.0614924682440978 0.0 56-57 0.0 0.0 0.0 1.2326364695272178 0.0 58-59 0.0 0.0 0.0 1.4190043725990802 0.0 60-61 0.0 0.0 0.0 1.6269328965116494 0.0 62-63 0.0 0.0 0.0 1.8303652421757297 0.0 64-65 0.0 0.0 0.0 2.0415541526544545 0.0 66-67 0.0 0.0 0.0 2.279325731023367 0.0 68-69 0.0 0.0 0.0 2.5453627574456448 0.0 70-71 0.0 0.0 0.0 2.835563455273544 0.0 72-73 0.0 0.0 0.0 3.1298922231892368 0.0 74-75 0.0 0.0 0.0 3.436500027608112 0.0 76-77 0.0 0.0 0.0 3.7615395335017867 0.0 78-79 0.0 0.0 0.0 4.122048890022429 0.0 80-81 0.0 0.0 0.0 4.518738020051377 0.0 82-83 0.0 0.0 0.0 4.921579815051943 0.0 84-85 0.0 0.0 0.0 5.321476744766948 0.0 86-87 0.0 0.0 0.0 5.7265271887316835 0.0 88-89 0.0 0.0 0.0 6.172674279494009 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8595 0.0 29.85264 1 GTATCAA 12265 0.0 28.474405 1 GTACATG 10630 0.0 24.539967 1 TACATGG 10650 0.0 23.99743 2 GGACCGA 500 0.0 23.748358 6 ATCAACG 14990 0.0 23.005558 3 TATCAAC 15095 0.0 22.973217 2 TCAACGC 15125 0.0 22.829826 4 CAACGCA 15275 0.0 22.636732 5 ACATGGG 10895 0.0 22.322382 3 AACGCAG 15695 0.0 22.03097 6 GAGTACT 7720 0.0 22.025679 12-13 GTACTTT 8020 0.0 20.964882 14-15 TATCACG 160 4.988087E-5 20.78309 2 AATACCG 115 0.003725655 20.650743 5 AGTACTT 8075 0.0 20.351532 12-13 CATGGGA 6045 0.0 19.87869 4 ACGCAGA 17405 0.0 19.83919 7 CGCAGAG 17435 0.0 19.696085 8 CATGGGG 4515 0.0 19.566753 4 >>END_MODULE