FastQCFastQC Report
Thu 26 May 2016
SRR938391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4073879
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62340.15302369068889873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49240.12086760554253083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89550.042.1622771
GGTATCA51800.040.208091
ATCAACG107450.034.515963
TCAACGC109050.033.922334
TATCAAC111350.033.3070452
CAACGCA111400.033.164055
AACGCAG117650.031.4214256
CGCAGAG137650.026.3424348
ACGCAGA138750.026.2366687
GTACATG121400.025.9303531
TACATGG118350.025.7526252
ACATGGG126600.023.2857233
GCAGAGT151950.022.893529
GAGTACT77950.020.33158712-13
AGAGTAC135150.019.86759410-11
CATGGGG90450.019.7130784
AGTACTT83450.019.30478312-13
GTACTTT82150.019.2861914-15
CAGAGTA142250.018.95948210-11
GAGTACA93200.017.398331
Produced by FastQC (version 0.11.5)