##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938391_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4073879 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.03581451486409 31.0 31.0 34.0 28.0 34.0 2 31.0792848781223 31.0 31.0 34.0 27.0 34.0 3 31.13282377802581 31.0 31.0 34.0 27.0 34.0 4 34.777793842183335 37.0 35.0 37.0 32.0 37.0 5 34.74335222032859 37.0 35.0 37.0 32.0 37.0 6 34.74998791078478 37.0 35.0 37.0 32.0 37.0 7 34.64869157871404 37.0 35.0 37.0 30.0 37.0 8 34.61541223978424 37.0 35.0 37.0 30.0 37.0 9 36.105778055754726 39.0 35.0 39.0 30.0 39.0 10-11 35.92520862794403 38.0 35.0 39.0 30.0 39.0 12-13 35.77303093685404 38.0 35.0 39.0 30.0 39.0 14-15 36.73758228950835 39.0 36.0 41.0 30.0 41.0 16-17 36.54508749523488 39.0 36.0 40.5 30.0 41.0 18-19 36.67255961701366 39.0 36.0 41.0 30.0 41.0 20-21 36.64021489101664 39.0 36.0 41.0 30.0 41.0 22-23 36.53813331225596 39.0 36.0 41.0 30.0 41.0 24-25 36.39462499990795 39.0 36.0 41.0 29.0 41.0 26-27 36.17628199070223 39.0 35.0 40.5 28.0 41.0 28-29 35.96755414188787 39.0 35.0 40.0 27.0 41.0 30-31 35.72861614201109 38.0 35.0 40.0 27.0 41.0 32-33 35.47550393617483 38.0 34.0 40.0 26.5 41.0 34-35 35.2245628797517 38.0 34.0 40.0 25.5 41.0 36-37 34.949577172026956 38.0 34.0 40.0 25.0 41.0 38-39 34.84147516408808 38.0 34.0 40.0 25.0 41.0 40-41 34.7499728146074 38.0 33.5 40.0 24.5 41.0 42-43 34.46316900919247 38.0 33.0 40.0 24.0 41.0 44-45 34.143767033826975 38.0 33.0 40.0 23.0 41.0 46-47 33.790096244881106 38.0 33.0 40.0 22.5 41.0 48-49 33.44951826011524 37.5 32.0 40.0 19.5 41.0 50-51 32.759123675494536 36.5 31.5 39.5 18.5 40.5 52-53 32.785930190857414 37.0 31.0 39.0 18.5 40.0 54-55 32.8752222390503 37.0 31.5 40.0 17.5 41.0 56-57 32.53320189431252 37.0 31.0 39.5 14.0 41.0 58-59 32.128159181949194 36.0 31.0 39.0 11.0 41.0 60-61 31.623544170064942 36.0 30.0 39.0 8.0 40.0 62-63 31.027491243603457 35.0 29.0 39.0 6.5 40.0 64-65 30.478404979627527 35.0 28.5 38.0 2.0 40.0 66-67 29.902349333399446 34.0 28.0 38.0 2.0 40.0 68-69 29.26923615060732 34.0 27.0 37.0 2.0 39.5 70-71 28.610325932606244 34.0 26.0 36.5 2.0 39.0 72-73 27.945148591796666 33.0 26.0 36.0 2.0 39.0 74-75 27.231872497931334 33.0 24.5 35.5 2.0 38.0 76-77 26.44026209418591 32.0 22.5 35.0 2.0 37.0 78-79 25.621285511916284 31.5 20.0 35.0 2.0 36.5 80-81 24.89269121640579 31.0 18.0 34.5 2.0 36.0 82-83 24.13685016172547 30.5 10.5 34.0 2.0 35.5 84-85 23.389627183330678 30.0 6.0 34.0 2.0 35.0 86-87 22.58675552219396 29.5 2.0 34.0 2.0 35.0 88-89 21.736770286991835 29.0 2.0 34.0 2.0 35.0 90-91 20.7894487047848 28.0 2.0 34.0 2.0 35.0 92-93 19.79633059793872 26.5 2.0 33.0 2.0 35.0 94-95 18.74673167759769 25.0 2.0 33.0 2.0 34.5 96-97 17.472348221437112 22.0 2.0 32.0 2.0 34.0 98-99 15.938950445018126 6.5 2.0 32.0 2.0 34.0 100-101 12.511816011226646 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.462857395464372 1101 2 0.43417105258278 1101 3 0.35725733593569586 1101 4 0.24438613309446566 1101 5 0.2695815527237144 1101 6 0.39957290446535865 1101 7 0.33584643904406875 1101 8 0.448307361779797 1101 9 0.5827720360469755 1101 10-11 0.603253583799372 1101 12-13 0.612647682831458 1101 14-15 0.8411218325808392 1101 16-17 0.604984101016818 1101 18-19 0.7541785609464924 1101 20-21 0.8254403085167112 1101 22-23 0.7893158390641872 1101 24-25 0.68241007861063 1101 26-27 0.7057457493630395 1101 28-29 0.7851834545277185 1101 30-31 0.777490491054941 1101 32-33 0.906965103896205 1101 34-35 0.816138262331215 1101 36-37 0.82145435724739 1101 38-39 0.8314614384059382 1101 40-41 0.9703385942377665 1101 42-43 0.9954822913823662 1101 44-45 0.9231307697122517 1101 46-47 0.8974288582027512 1101 48-49 0.7010848875435371 1101 50-51 0.8851362668768132 1101 52-53 0.8799930028359029 1101 54-55 0.9096006262360845 1101 56-57 0.9752476235938659 1101 58-59 0.883402338502357 1101 60-61 0.8930971512005712 1101 62-63 0.7593449374832879 1101 64-65 0.8023091725655007 1101 66-67 0.9517047743826801 1101 68-69 0.8189943506094863 1101 70-71 1.0225902102349487 1101 72-73 1.1506085445764853 1101 74-75 1.0291346827643935 1101 76-77 1.2458639077016294 1101 78-79 1.4137268374224945 1101 80-81 1.3919306366351485 1101 82-83 1.34350056966829 1101 84-85 1.411421319338654 1101 86-87 1.5952195726906595 1101 88-89 1.5815473548579284 1101 90-91 1.4959524702734122 1101 92-93 1.4459501721479278 1101 94-95 1.4292553212137662 1101 96-97 1.2754094486609127 1101 98-99 1.3587304101004047 1101 100-101 1.6467274063019488 1102 1 0.48589572653457935 1102 2 0.3974382320926182 1102 3 0.4304277251746207 1102 4 0.4030644281109801 1102 5 0.3561989068533151 1102 6 0.4503221959320314 1102 7 0.3770331552405466 1102 8 0.563782610808282 1102 9 0.7148314929460824 1102 10-11 0.7070326708299746 1102 12-13 0.6311381773871432 1102 14-15 0.7980423878344567 1102 16-17 0.8874225029629059 1102 18-19 0.8087953668250378 1102 20-21 0.7408330364869329 1102 22-23 0.8167125148026741 1102 24-25 0.8022074421362433 1102 26-27 0.6634623700393973 1102 28-29 0.5751930605147848 1102 30-31 0.736669812303127 1102 32-33 0.7380590798718316 1102 34-35 0.590975348938521 1102 36-37 0.8950836941921025 1102 38-39 0.7770316446196972 1102 40-41 0.9231757032594672 1102 42-43 1.0433930375023337 1102 44-45 1.1173761432321143 1102 46-47 1.0789164451986295 1102 48-49 1.1093133487550872 1102 50-51 1.1824892937932887 1102 52-53 1.0428601497289236 1102 54-55 1.0101840833531526 1102 56-57 1.175247539861914 1102 58-59 1.1025294986095702 1102 60-61 0.931889745483069 1102 62-63 1.09161399477874 1102 64-65 1.2677864891290866 1102 66-67 0.9485393201761347 1102 68-69 0.9473588775297586 1102 70-71 1.2298551292397448 1102 72-73 1.2152751812714087 1102 74-75 1.309124279391515 1102 76-77 1.341821303339092 1102 78-79 1.2143143007913793 1102 80-81 1.439893232155594 1102 82-83 1.5350056218609893 1102 84-85 1.6137095976255296 1102 86-87 1.769101206885857 1102 88-89 1.6855734189396685 1102 90-91 1.4858055748029635 1102 92-93 1.6337363663465396 1102 94-95 1.5515727910351629 1102 96-97 1.7161018822682372 1102 98-99 1.7664615357270588 1102 100-101 1.48427347658758 1103 1 0.4449792897650937 1103 2 0.42665139196685686 1103 3 0.3512767997177626 1103 4 0.34579427948622765 1103 5 0.3213900966287966 1103 6 0.4055110632136376 1103 7 0.32498490382092626 1103 8 0.3888230563705619 1103 9 0.47412214934029606 1103 10-11 0.6685907237679771 1103 12-13 0.5967462647168773 1103 14-15 0.7043513322585113 1103 16-17 0.7837575680150124 1103 18-19 0.724542351916007 1103 20-21 0.5982691146712895 1103 22-23 0.6694139119139706 1103 24-25 0.7187198536128321 1103 26-27 0.6910136964111899 1103 28-29 0.809156958362685 1103 30-31 0.8472403906140116 1103 32-33 0.9581531024956007 1103 34-35 0.8965748742386026 1103 36-37 0.8484049099507871 1103 38-39 0.9240231298817676 1103 40-41 0.8705829161628316 1103 42-43 0.8534817788047349 1103 44-45 0.7442870320143697 1103 46-47 0.6594942930639007 1103 48-49 0.7865498076006574 1103 50-51 0.9982495131377291 1103 52-53 1.0294522663230765 1103 54-55 1.0999477596975282 1103 56-57 1.0726624840948276 1103 58-59 0.9371565142643661 1103 60-61 1.1935212800968955 1103 62-63 1.1115843780298462 1103 64-65 1.1459160062930067 1103 66-67 1.1889464362676527 1103 68-69 1.272978553806798 1103 70-71 1.3513398193502297 1103 72-73 1.2821525746409606 1103 74-75 1.4477153563106313 1103 76-77 1.3446741887034683 1103 78-79 1.4463811445420127 1103 80-81 1.4953476015886018 1103 82-83 1.5116111695510988 1103 84-85 1.6794377526888908 1103 86-87 1.7760370884753165 1103 88-89 1.8005375465609532 1103 90-91 1.5890894833373146 1103 92-93 1.5999427496392435 1103 94-95 1.6249207006173556 1103 96-97 1.8044453523712036 1103 98-99 1.821946576687628 1103 100-101 1.6958832471063676 1104 1 0.40997482670055163 1104 2 0.3363578374958749 1104 3 0.3322789016771921 1104 4 0.25632063316250253 1104 5 0.368486225280229 1104 6 0.5304516756612685 1104 7 0.37954730754110955 1104 8 0.5448719485227684 1104 9 0.6547499297575214 1104 10-11 0.7069379861817779 1104 12-13 0.6791477533648518 1104 14-15 0.8349357417662873 1104 16-17 0.9294247961639925 1104 18-19 0.8771260703686252 1104 20-21 0.8046386996062793 1104 22-23 0.8344414821352473 1104 24-25 0.7443133692119588 1104 26-27 0.904080037706052 1104 28-29 0.8588200689747012 1104 30-31 0.956573594374504 1104 32-33 1.0220042592931335 1104 34-35 0.8810912796000352 1104 36-37 0.9583280300648198 1104 38-39 1.0735172559129538 1104 40-41 1.1672323784355356 1104 42-43 1.1897148443541923 1104 44-45 1.1281744692075364 1104 46-47 0.9387925121571783 1104 48-49 0.7722541439717503 1104 50-51 1.086403496062907 1104 52-53 1.1482594043469376 1104 54-55 1.1280184560795732 1104 56-57 1.2220835454403698 1104 58-59 1.171652223963612 1104 60-61 1.0823981715004578 1104 62-63 1.1926071756514673 1104 64-65 1.2317186020689341 1104 66-67 1.2674943926160012 1104 68-69 1.3381484741914846 1104 70-71 1.485216998693442 1104 72-73 1.573103891245001 1104 74-75 1.3702212590734106 1104 76-77 1.3390084362451837 1104 78-79 1.4493955127304403 1104 80-81 1.5325530033363997 1104 82-83 1.5865379249031761 1104 84-85 1.6823256073945458 1104 86-87 1.8466793018673897 1104 88-89 1.7153034516272534 1104 90-91 1.7538213755327092 1104 92-93 1.8914472702917102 1104 94-95 1.835045738409434 1104 96-97 1.8458615115442427 1104 98-99 2.0037111788270234 1104 100-101 1.963381339506201 1105 1 0.38631806286525716 1105 2 0.3217066062283429 1105 3 0.35849858921539735 1105 4 0.3321519389208163 1105 5 0.3535579351415663 1105 6 0.3873970025959039 1105 7 0.4481840366966878 1105 8 0.4102512987689195 1105 9 0.5953403458279709 1105 10-11 0.6192675364303355 1105 12-13 0.6089199589704322 1105 14-15 0.6756537530036155 1105 16-17 0.7535254740886543 1105 18-19 0.6437091372329675 1105 20-21 0.7542141572527825 1105 22-23 0.7375715241676133 1105 24-25 0.7805537543737344 1105 26-27 0.7522095028911764 1105 28-29 0.5898971547079768 1105 30-31 0.7544043904409179 1105 32-33 0.7775958893256956 1105 34-35 0.7158012115320602 1105 36-37 0.837800016812821 1105 38-39 0.8305547062136824 1105 40-41 0.96677288086088 1105 42-43 0.9401307770473153 1105 44-45 0.9694045830444651 1105 46-47 1.0299545235804715 1105 48-49 0.9362239206199376 1105 50-51 0.9938405207020296 1105 52-53 1.0525923881280619 1105 54-55 1.0258357349791893 1105 56-57 0.8452418960360717 1105 58-59 0.9688322158016618 1105 60-61 0.9741407323234057 1105 62-63 1.0996217242555844 1105 64-65 1.3871687753813973 1105 66-67 1.4898593630073798 1105 68-69 1.3098026310216149 1105 70-71 1.4190359168723923 1105 72-73 1.4332679397139465 1105 74-75 1.511218998176595 1105 76-77 1.582414211260982 1105 78-79 1.4935716801573378 1105 80-81 1.5091515159314675 1105 82-83 1.5113737817279684 1105 84-85 1.4235927427711204 1105 86-87 1.6865467647554482 1105 88-89 1.6335754755689926 1105 90-91 1.6674744888719992 1105 92-93 1.5652201784455784 1105 94-95 1.5953598532588629 1105 96-97 1.8545285122657305 1105 98-99 1.7930771289977496 1105 100-101 1.6655098197461697 1106 1 0.28531014515426634 1106 2 0.19278971536705214 1106 3 0.12388430190732436 1106 4 0.1413777712513351 1106 5 0.28521745227786965 1106 6 0.040752342345506065 1106 7 0.3233710358782602 1106 8 0.2696481832939952 1106 9 0.08014629505480286 1106 10-11 0.046696609597418615 1106 12-13 0.1850082503476429 1106 14-15 0.5080505361833474 1106 16-17 0.31426761152145133 1106 18-19 0.13293825689967065 1106 20-21 0.27468182766411786 1106 22-23 0.43593603183792595 1106 24-25 0.2869341673006218 1106 26-27 0.1530365319483309 1106 28-29 0.2907963177998809 1106 30-31 0.04240501724905954 1106 32-33 0.40936492755844256 1106 34-35 -0.040068259079070856 1106 36-37 0.18661687386395442 1106 38-39 0.2711201791344635 1106 40-41 0.4892360324624363 1106 42-43 -0.013401618969389517 1106 44-45 0.18279690106783875 1106 46-47 0.3469420461809136 1106 48-49 0.6102639290341543 1106 50-51 0.3533213770786503 1106 52-53 0.18884940671854622 1106 54-55 0.39391391226199346 1106 56-57 -0.4720897748930639 1106 58-59 -0.10688475988312263 1106 60-61 0.6525515352254558 1106 62-63 0.5660184205678895 1106 64-65 0.5469442997157756 1106 66-67 0.15435570648461194 1106 68-69 0.3333609084233906 1106 70-71 0.12172037603320618 1106 72-73 0.6015231732638533 1106 74-75 0.8621354070088003 1106 76-77 0.8444877198451017 1106 78-79 0.91847418849704 1106 80-81 1.0947281155484028 1106 82-83 0.8448016472674027 1106 84-85 0.7947229476845834 1106 86-87 0.7166200996047039 1106 88-89 0.6956064020782939 1106 90-91 0.7553361277879276 1106 92-93 0.7460486212276187 1106 94-95 0.7408754923774765 1106 96-97 0.8178598953400389 1106 98-99 0.9297114247711402 1106 100-101 0.4396292863120017 1107 1 0.23025105988040906 1107 2 0.07561808004697568 1107 3 0.21712051676747635 1107 4 0.1397307041266984 1107 5 0.14211411520157924 1107 6 0.31623888561030356 1107 7 0.2757829840192727 1107 8 0.20635199841058238 1107 9 0.2635126204452405 1107 10-11 0.44067317503882464 1107 12-13 0.4889339468328515 1107 14-15 0.5226933868293884 1107 16-17 0.5862406247353604 1107 18-19 0.48070069534619364 1107 20-21 0.6387862766030494 1107 22-23 0.5554636362948528 1107 24-25 0.559826630834344 1107 26-27 0.4344513096336797 1107 28-29 0.5652383330881321 1107 30-31 0.5556073801087749 1107 32-33 0.6141725069667672 1107 34-35 0.5102408795278066 1107 36-37 0.6055987450455405 1107 38-39 0.5602291069449805 1107 40-41 0.6257367135265355 1107 42-43 0.7436115221776376 1107 44-45 0.595188712202237 1107 46-47 0.727689017223426 1107 48-49 0.7628773850527395 1107 50-51 0.602417196265641 1107 52-53 0.7413131719584243 1107 54-55 0.904743548114574 1107 56-57 0.8157885519438395 1107 58-59 0.7278963376801997 1107 60-61 0.549317955319907 1107 62-63 0.8633791662490182 1107 64-65 0.8421846786861913 1107 66-67 0.981649949880552 1107 68-69 0.9463383354618848 1107 70-71 1.0848653542121411 1107 72-73 1.2808380078928856 1107 74-75 1.129048260019676 1107 76-77 1.2911599320438398 1107 78-79 1.2612579132975306 1107 80-81 1.2714135080326052 1107 82-83 1.0344610490665573 1107 84-85 1.3093363618084908 1107 86-87 1.3407681079814324 1107 88-89 1.3443620475236564 1107 90-91 1.339135732324241 1107 92-93 1.4964829753026727 1107 94-95 1.6085327476257518 1107 96-97 1.5342655471522875 1107 98-99 1.349929133760801 1107 100-101 1.3871351749945404 1108 1 0.292099924676851 1108 2 0.1811195631668454 1108 3 0.21359707175886555 1108 4 0.3536335954769214 1108 5 0.22800043536556558 1108 6 0.24655877762734946 1108 7 0.237075596309964 1108 8 0.3441359833577593 1108 9 0.2771305050616846 1108 10-11 0.4772987977331766 1108 12-13 0.42979190977958837 1108 14-15 0.5460853437366779 1108 16-17 0.5387588103714975 1108 18-19 0.5417462328698193 1108 20-21 0.47440961190290665 1108 22-23 0.4804715372089987 1108 24-25 0.5276418145128687 1108 26-27 0.6052991992532242 1108 28-29 0.5374157200059884 1108 30-31 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2316 92-93 -3.3606085814831665 2316 94-95 -3.676861773903145 2316 96-97 -3.628571369977557 2316 98-99 -3.453297364732352 2316 100-101 -2.9051606729131514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16406.0 3 6033.0 4 6482.0 5 7419.0 6 8748.0 7 10547.0 8 13078.0 9 16603.0 10 20030.0 11 24243.0 12 28623.0 13 32660.0 14 36172.0 15 40684.0 16 45086.0 17 50732.0 18 52382.0 19 52815.0 20 57297.0 21 63179.0 22 69875.0 23 77262.0 24 85884.0 25 95398.0 26 106399.0 27 119612.0 28 135097.0 29 152995.0 30 175564.0 31 202283.0 32 235870.0 33 277111.0 34 328823.0 35 393972.0 36 447867.0 37 409853.0 38 164274.0 39 6521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34123460087471 17.961546619115047 13.94491075233552 24.75230802767473 2 15.847110951228165 19.249128424992456 39.57053737378503 25.333223249994347 3 19.708015205739233 22.57819801324715 28.43708661024517 29.276700170768443 4 13.37709177181127 15.490428240928466 35.27663462564 35.85584536162026 5 14.840745367391639 36.200566600375616 33.54357323087355 15.415114801359191 6 34.60052222400496 34.17830065153919 16.743281961895388 14.477895162560465 7 30.655742857030617 30.826779309450757 19.70685638634301 18.810621447175617 8 26.935844727780584 34.925642269683536 19.078906015207437 19.059606987328443 9 26.661648202203853 16.630881872368466 19.19594574853319 37.51152417689449 10-11 25.807191165324987 25.604085590212026 26.804655661144256 21.78406758331872 12-13 26.378985111166752 22.715875065100192 27.11028086320185 23.7948589605312 14-15 23.245478130074872 24.39339691344596 24.825351935615192 27.535773020863974 16-17 23.50131636650433 27.53086868403128 25.521635702629347 23.446179246835047 18-19 23.447300656635363 26.50354469647328 27.127619018152043 22.921535628739317 20-21 23.805946046182623 25.806832709271525 27.28351973316599 23.103701511379867 22-23 23.628458610606174 25.54121456491788 27.12840078447107 23.70192604000488 24-25 23.564438866792493 25.79912348454801 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50-51 23.714748950752217 26.21574716764778 26.51068911395531 23.558814767644687 52-53 23.672303256667803 26.10748541754353 26.48971449059384 23.730496835194828 54-55 23.407466325921263 26.107861386831345 26.710757354534177 23.773914932713218 56-57 23.46384103541012 26.343949213466427 26.637925732906698 23.554284018216748 58-59 23.514322018844947 25.953126643795365 26.729897253467676 23.802654083892012 60-61 23.4516550945051 26.13966292391395 26.723983629150013 23.68469835243094 62-63 23.27817330936331 26.194518072156058 26.810020373538606 23.71728824494203 64-65 23.213754407740144 26.07115081909584 26.777493938897013 23.937600834267005 66-67 23.308220378636157 26.136392091051793 26.631511475987747 23.923876054324307 68-69 23.26655433658004 25.94896194504287 26.85842076107459 23.926062957302495 70-71 23.34447111028609 25.996862957708366 26.65815618718733 24.000509744818206 72-73 23.39541577706057 25.958046072050696 26.639947484850147 24.006590666038583 74-75 23.252491477364803 26.31076950728316 26.434717252799384 24.002021762552655 76-77 23.540743419680442 26.113061594729974 26.40733439129627 23.93886059429331 78-79 23.565146170607594 26.064826128404984 26.53584969762609 23.834178003361334 80-81 23.437494630165045 26.27966542307426 26.390621555634436 23.892218391126256 82-83 23.598096542649817 26.250625975785784 26.237333195864142 23.913944285700257 84-85 23.545013419126718 26.121061069607375 26.278474679913383 24.05545083135252 86-87 23.621930970331317 26.00214247021882 26.333615288993506 24.042311270456363 88-89 23.604243493165193 26.05322108044429 26.22209153063248 24.120443895758044 90-91 23.60023376706291 26.08037461662938 26.218694980638098 24.100696635669614 92-93 23.67533168117847 25.725354995173916 26.321157100971575 24.278156222676035 94-95 23.55754088583719 25.83088294793305 26.32467239205708 24.28690377417268 96-97 23.72207739617126 25.721573236980728 26.160790928277894 24.395558438570117 98-99 23.717444754847104 25.71729940194154 25.98410300806856 24.5811528351428 100-101 24.420200815268526 25.07769960052137 26.12471584251374 24.377383741696363 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 260.0 1 209.5 2 212.5 3 287.0 4 268.5 5 267.0 6 481.5 7 961.0 8 1518.0 9 1760.0 10 1593.0 11 1472.5 12 1561.5 13 1674.0 14 1808.0 15 1974.0 16 2199.0 17 2417.0 18 2623.0 19 2869.0 20 3221.0 21 3874.0 22 4695.0 23 5786.0 24 7391.0 25 9482.5 26 12342.5 27 16017.5 28 19546.5 29 23663.0 30 29210.5 31 36127.5 32 45218.0 33 52399.5 34 57913.0 35 64478.0 36 73376.5 37 82513.0 38 89460.5 39 96085.0 40 102771.0 41 113191.5 42 123318.5 43 130924.5 44 138580.5 45 147373.5 46 154679.5 47 160858.5 48 164603.5 49 164872.0 50 165181.5 51 162786.0 52 162240.5 53 164755.0 54 166160.0 55 157604.5 56 141645.5 57 128394.5 58 117307.0 59 104119.0 60 87541.5 61 73598.5 62 61613.5 63 49205.0 64 38926.0 65 30802.5 66 23887.0 67 18778.0 68 14572.0 69 11052.0 70 8538.5 71 6357.5 72 4898.0 73 3875.5 74 2991.0 75 2329.5 76 1611.5 77 1051.0 78 682.0 79 434.0 80 260.0 81 157.5 82 91.0 83 49.0 84 32.0 85 24.0 86 17.5 87 13.5 88 10.5 89 6.5 90 2.5 91 2.5 92 2.5 93 4.0 94 3.5 95 1.0 96 0.0 97 0.5 98 0.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006652136698218086 2 0.10395497755333431 3 0.11355270983747921 4 0.11588463967633796 5 0.1586203223021597 6 0.09764649367347435 7 0.02609306756533515 8 0.027860424916891252 9 0.02258289949210568 10-11 0.05167065590313311 12-13 0.055733123148723855 14-15 0.02741858557900222 16-17 0.03203335199695425 18-19 0.037494977145860246 20-21 0.05974649713454916 22-23 0.03254883122449145 24-25 0.050909710376768676 26-27 0.058273699341585744 28-29 0.054395331820115425 30-31 0.06914785637963228 32-33 0.05099562358135821 34-35 0.0629130123894205 36-37 0.05330300679033422 38-39 0.032499737964726 40-41 0.037335424051622546 42-43 0.032401551445195104 44-45 0.0074376288544652416 46-47 0.013181540247022555 48-49 0.008836786757780483 50-51 0.008971793222135463 52-53 0.009953658417444406 54-55 0.011205536541463309 56-57 0.01459297146527916 58-59 0.019575937331472042 60-61 0.025283028779205272 62-63 0.011242356486287393 64-65 0.008910426647428655 66-67 0.010235944661095727 68-69 0.011475549470173266 70-71 0.030756927243052627 72-73 0.028216351050190738 74-75 0.02933322270985466 76-77 0.023503398112707814 78-79 0.004847959401837904 80-81 0.0048356860868965425 82-83 0.005866644541970932 84-85 0.00530207205466829 86-87 0.01334109334126026 88-89 0.037470430515977524 90-91 0.035568066700066445 92-93 0.029897795197157306 94-95 0.04665087009211614 96-97 0.04301796886947305 98-99 0.025356668668853444 100-101 0.07543179362960953 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4073879.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.216455386922945 #Duplication Level Percentage of deduplicated Percentage of total 1 74.12230976744982 36.480373518453774 2 13.5163625966122 13.304549134592767 3 4.941912734202139 7.296702828267779 4 2.345795832100871 4.6180702366968935 5 1.33190876007472 3.277591403483466 6 0.7834800931291503 2.3136067830019837 7 0.5595748877092607 1.9278204747609673 8 0.38816951846619335 1.5283462230523814 9 0.2902194837294208 1.2855216846046362 >10 1.5114386203091006 13.46997090926349 >50 0.12259712032446107 4.162531509964289 >100 0.0798633941217136 7.25581470274475 >500 0.004662589321172049 1.5415613303030504 >1k 0.0016544670834756179 1.383716245148483 >5k 5.013536616592737E-5 0.15382301566132167 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6234 0.15302369068889873 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4924 0.12086760554253083 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4546629882723567E-5 2 0.0 0.0 0.0 0.0 4.9093259765447134E-5 3 0.0 0.0 0.0 0.0 4.9093259765447134E-5 4 0.0 0.0 0.0 0.0 4.9093259765447134E-5 5 0.0 0.0 0.0 0.0 4.9093259765447134E-5 6 0.0 0.0 0.0 0.0 4.9093259765447134E-5 7 0.0 0.0 0.0 0.0 4.9093259765447134E-5 8 0.0 0.0 0.0 0.0 4.9093259765447134E-5 9 0.0 0.0 0.0 4.9093259765447134E-5 4.9093259765447134E-5 10-11 0.0 0.0 0.0 4.9093259765447134E-5 4.9093259765447134E-5 12-13 0.0 0.0 0.0 4.9093259765447134E-5 4.9093259765447134E-5 14-15 0.0 0.0 0.0 7.36398896481707E-5 4.9093259765447134E-5 16-17 0.0 0.0 0.0 7.36398896481707E-5 4.9093259765447134E-5 18-19 0.0 0.0 0.0 7.36398896481707E-5 4.9093259765447134E-5 20-21 0.0 0.0 0.0 9.818651953089427E-5 4.9093259765447134E-5 22-23 0.0 0.0 0.0 2.086463540031503E-4 7.36398896481707E-5 24-25 0.0 0.0 0.0 4.2956602294766237E-4 7.36398896481707E-5 26-27 0.0 0.0 0.0 7.732188413057923E-4 7.36398896481707E-5 28-29 0.0 0.0 0.0 0.002295109894034653 7.36398896481707E-5 30-31 0.0 0.0 0.0 0.008922699962370016 7.36398896481707E-5 32-33 0.0 0.0 0.0 0.024755276236726718 7.36398896481707E-5 34-35 0.0 0.0 0.0 0.046761329926588394 7.36398896481707E-5 36-37 0.0 0.0 0.0 0.0868091565802519 7.36398896481707E-5 38-39 0.0 2.4546629882723567E-5 0.0 0.1500167285282651 7.36398896481707E-5 40-41 0.0 2.4546629882723567E-5 0.0 0.21992553043426177 7.36398896481707E-5 42-43 0.0 2.4546629882723567E-5 0.0 0.29071801101603656 7.36398896481707E-5 44-45 1.2273314941361784E-5 2.4546629882723567E-5 0.0 0.3780303735088843 8.591320458953248E-5 46-47 2.4546629882723567E-5 2.4546629882723567E-5 0.0 0.4835686086896543 9.818651953089427E-5 48-49 2.4546629882723567E-5 4.9093259765447134E-5 0.0 0.599207782067165 9.818651953089427E-5 50-51 4.9093259765447134E-5 4.9093259765447134E-5 0.0 0.7228614301013849 9.818651953089427E-5 52-53 4.9093259765447134E-5 4.9093259765447134E-5 0.0 0.8467728177493735 1.2273314941361785E-4 54-55 4.9093259765447134E-5 4.9093259765447134E-5 0.0 0.983301173157082 1.472797792963414E-4 56-57 7.36398896481707E-5 4.9093259765447134E-5 0.0 1.1451371039738785 1.472797792963414E-4 58-59 7.36398896481707E-5 4.9093259765447134E-5 0.0 1.320682327580176 1.472797792963414E-4 60-61 7.36398896481707E-5 4.9093259765447134E-5 0.0 1.5102804967943328 1.472797792963414E-4 62-63 7.36398896481707E-5 4.9093259765447134E-5 0.0 1.6988722541832981 1.7182640917906496E-4 64-65 7.36398896481707E-5 6.136657470680892E-5 0.0 1.9005473652997549 1.7182640917906496E-4 66-67 7.36398896481707E-5 7.36398896481707E-5 0.0 2.1339735421695147 1.7182640917906496E-4 68-69 7.36398896481707E-5 7.36398896481707E-5 0.0 2.3894180460440775 1.7182640917906496E-4 70-71 7.36398896481707E-5 7.36398896481707E-5 0.0 2.6624624835445534 2.209196689445121E-4 72-73 7.36398896481707E-5 7.36398896481707E-5 0.0 2.9415453919961783 2.209196689445121E-4 74-75 7.36398896481707E-5 7.36398896481707E-5 0.0 3.2258199126680003 2.209196689445121E-4 76-77 7.36398896481707E-5 7.36398896481707E-5 0.0 3.5392558296404975 2.209196689445121E-4 78-79 7.36398896481707E-5 9.818651953089427E-5 0.0 3.886222443032795 2.209196689445121E-4 80-81 7.36398896481707E-5 9.818651953089427E-5 0.0 4.253305019613984 2.209196689445121E-4 82-83 7.36398896481707E-5 9.818651953089427E-5 0.0 4.630390347872384 2.4546629882723565E-4 84-85 7.36398896481707E-5 9.818651953089427E-5 0.0 5.008518907900799 2.4546629882723565E-4 86-87 7.36398896481707E-5 9.818651953089427E-5 0.0 5.40796621598236 2.700129287099592E-4 88-89 7.36398896481707E-5 1.1045983447225605E-4 0.0 5.836022621192235 2.700129287099592E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8955 0.0 42.162277 1 GGTATCA 5180 0.0 40.20809 1 ATCAACG 10745 0.0 34.51596 3 TCAACGC 10905 0.0 33.92233 4 TATCAAC 11135 0.0 33.307045 2 CAACGCA 11140 0.0 33.16405 5 AACGCAG 11765 0.0 31.421425 6 CGCAGAG 13765 0.0 26.342434 8 ACGCAGA 13875 0.0 26.236668 7 GTACATG 12140 0.0 25.930353 1 TACATGG 11835 0.0 25.752625 2 ACATGGG 12660 0.0 23.285723 3 GCAGAGT 15195 0.0 22.89352 9 GAGTACT 7795 0.0 20.331587 12-13 AGAGTAC 13515 0.0 19.867594 10-11 CATGGGG 9045 0.0 19.713078 4 AGTACTT 8345 0.0 19.304783 12-13 GTACTTT 8215 0.0 19.28619 14-15 CAGAGTA 14225 0.0 18.959482 10-11 GAGTACA 9320 0.0 17.39833 1 >>END_MODULE