FastQCFastQC Report
Thu 26 May 2016
SRR938391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4073879
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67190.16492880618201963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52060.1277897551694589No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51200.043.06971
GTATCAA81500.040.2360461
ATCAACG102350.031.4207533
TCAACGC105450.030.5871494
TATCAAC107700.030.0066172
CAACGCA108400.029.7109245
AACGCAG114700.028.1087076
TACATGG116100.024.5608162
GTACATG119800.023.9212721
CGCAGAG135700.023.5138598
ACGCAGA138700.023.176447
ACATGGG120200.022.6841873
GCAGAGT152950.020.3341649
GAGTACT77600.019.24391412-13
CATGGGG83950.019.0122854
GGACCGA15500.018.6867036
AGAGTAC136950.018.6532610-11
AGGACCG18300.018.1703265
AGTACTT82350.018.04742412-13
GTACTTT82900.017.7272214-15
Produced by FastQC (version 0.11.5)