##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938391_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4073879 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79973018344433 31.0 31.0 34.0 30.0 34.0 2 32.02972007759681 31.0 31.0 34.0 30.0 34.0 3 32.100072437104785 33.0 31.0 34.0 30.0 34.0 4 35.76087139554218 37.0 35.0 37.0 35.0 37.0 5 35.65303068647842 37.0 35.0 37.0 33.0 37.0 6 35.64875196342356 37.0 35.0 37.0 33.0 37.0 7 35.58635737585726 37.0 35.0 37.0 33.0 37.0 8 35.56191801474712 37.0 35.0 37.0 33.0 37.0 9 37.154989630276205 39.0 37.0 39.0 33.0 39.0 10-11 37.05264761177247 39.0 37.0 39.0 33.0 39.0 12-13 36.93741137132448 39.0 37.0 39.0 33.0 39.0 14-15 38.007604177738216 40.0 37.0 41.0 33.0 41.0 16-17 37.903105369599835 40.0 37.0 41.0 33.0 41.0 18-19 38.12567052187853 40.0 37.0 41.0 33.0 41.0 20-21 38.08757083850551 40.0 37.0 41.0 33.0 41.0 22-23 37.976715312359545 40.0 37.0 41.0 33.0 41.0 24-25 37.85513254075538 40.0 37.0 41.0 32.0 41.0 26-27 37.660812459083836 40.0 37.0 41.0 32.0 41.0 28-29 37.55227548486344 39.5 37.0 41.0 32.0 41.0 30-31 37.332493552214 39.0 36.0 41.0 31.0 41.0 32-33 37.16292175098966 39.0 36.0 41.0 31.0 41.0 34-35 37.09946429434944 39.0 36.0 41.0 31.0 41.0 36-37 37.082950426362686 39.0 36.0 41.0 31.0 41.0 38-39 36.9336599098795 39.0 36.0 41.0 30.0 41.0 40-41 36.78286321218671 39.0 35.0 41.0 30.0 41.0 42-43 36.595233559956 39.0 35.0 40.0 30.0 41.0 44-45 36.33260487118051 39.0 35.0 40.0 30.0 41.0 46-47 35.998235710977184 38.0 35.0 40.0 29.0 41.0 48-49 35.681424018730056 38.0 34.5 40.0 28.0 41.0 50-51 35.315684142803455 38.0 34.0 40.0 27.0 41.0 52-53 34.83050760712334 38.0 33.0 40.0 26.0 41.0 54-55 34.48917039509519 38.0 33.0 40.0 26.0 41.0 56-57 34.14021231362051 37.0 33.0 40.0 25.0 41.0 58-59 33.64710414815953 36.5 32.0 39.0 23.0 41.0 60-61 33.235175983380955 36.0 31.5 39.0 22.5 40.0 62-63 32.73384567877446 35.5 31.0 39.0 22.0 40.0 64-65 32.190349173355415 35.0 31.0 38.0 20.5 40.0 66-67 31.660394429976932 35.0 30.0 38.0 18.5 40.0 68-69 31.134033190480128 34.0 29.5 37.5 17.5 39.5 70-71 30.53099159302473 34.0 29.0 37.0 13.0 39.0 72-73 30.001777298736656 34.0 28.5 36.0 9.5 39.0 74-75 29.384563090852723 33.5 28.0 36.0 7.5 38.5 76-77 28.16645879762261 31.5 26.5 34.5 7.0 36.0 78-79 28.847827709168584 33.0 28.0 35.0 4.5 37.0 80-81 28.454452500921114 33.0 27.0 35.0 2.0 36.5 82-83 27.99802375082814 33.0 26.0 35.0 2.0 36.0 84-85 27.420772806457926 32.5 26.0 35.0 2.0 35.5 86-87 26.781152557550186 32.0 25.0 34.5 2.0 35.0 88-89 26.003889781704366 31.5 23.5 34.0 2.0 35.0 90-91 25.3581528808293 31.0 21.5 34.0 2.0 35.0 92-93 24.48125570248895 31.0 18.5 34.0 2.0 35.0 94-95 23.61912835408219 30.5 9.5 34.0 2.0 35.0 96-97 22.49051186841828 30.0 2.0 34.0 2.0 35.0 98-99 21.06457261003579 29.0 2.0 34.0 2.0 35.0 100-101 17.095809301159903 22.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2808124440540425 1101 2 0.2775772711857485 1101 3 0.2767175813900238 1101 4 0.21959175189977742 1101 5 0.11408492785024293 1101 6 0.18499054627464773 1101 7 0.17654304078722305 1101 8 0.18822212617527612 1101 9 0.2945521540938216 1101 10-11 0.26454717472876865 1101 12-13 0.354219485511976 1101 14-15 0.462781761553245 1101 16-17 0.45587659152424465 1101 18-19 0.4497128520982301 1101 20-21 0.3859405677040897 1101 22-23 0.3283800969612187 1101 24-25 0.39798840540754554 1101 26-27 0.339822852326229 1101 28-29 0.24896280981875663 1101 30-31 0.3401422682693038 1101 32-33 0.2903472399677156 1101 34-35 0.3409404864233281 1101 36-37 0.357446125441399 1101 38-39 0.46606839994441884 1101 40-41 0.4420646188078905 1101 42-43 0.4725247768000145 1101 44-45 0.3827781471612539 1101 46-47 0.2282131196695687 1101 48-49 0.399931815733936 1101 50-51 0.4139321946121939 1101 52-53 0.4632516698686473 1101 54-55 0.49299515984444753 1101 56-57 0.32015079989468376 1101 58-59 0.48760495987706776 1101 60-61 0.5085247261651773 1101 62-63 0.5451610495651309 1101 64-65 0.4215301829030125 1101 66-67 0.44721094557595364 1101 68-69 0.5165580655213731 1101 70-71 0.5469939291410846 1101 72-73 0.5248770044060791 1101 74-75 0.5240434052884844 1101 76-77 0.5011363826665836 1101 78-79 0.6801879867576872 1101 80-81 0.7870940700705944 1101 82-83 0.6569366248078765 1101 84-85 0.6759050014309764 1101 86-87 0.7756110730059405 1101 88-89 0.958779976244152 1101 90-91 0.9612241063919811 1101 92-93 1.367056108969738 1101 94-95 1.3033447424644606 1101 96-97 1.2967694286408467 1101 98-99 1.2162612205183478 1101 100-101 1.2938073169809172 1102 1 0.2969191221666492 1102 2 0.25847060608379735 1102 3 0.24405960850448594 1102 4 0.2653962207899454 1102 5 0.09071205543081362 1102 6 0.11316882113109017 1102 7 0.16724164479525427 1102 8 0.17787343670268996 1102 9 0.3043417717698702 1102 10-11 0.34579811590810294 1102 12-13 0.4038269630070843 1102 14-15 0.47769442204324264 1102 16-17 0.44936633571282414 1102 18-19 0.38550506649530547 1102 20-21 0.3319818627565425 1102 22-23 0.3620385700485471 1102 24-25 0.4233979931281766 1102 26-27 0.28988476244268213 1102 28-29 0.2529460997553201 1102 30-31 0.2947638017826293 1102 32-33 0.35304630463029696 1102 34-35 0.37414777311263947 1102 36-37 0.42786295726397583 1102 38-39 0.4049319345122484 1102 40-41 0.23357747095388248 1102 42-43 0.20551385802477995 1102 44-45 0.3758602777709896 1102 46-47 0.5153651473933465 1102 48-49 0.5067996134174493 1102 50-51 0.6012385403218374 1102 52-53 0.5470159865830837 1102 54-55 0.3848963092522695 1102 56-57 0.550270515973807 1102 58-59 0.6559642786699911 1102 60-61 0.587207447381445 1102 62-63 0.6679995253499129 1102 64-65 0.7431275708268288 1102 66-67 0.6739401939322605 1102 68-69 0.7942242293240014 1102 70-71 0.6863021576602613 1102 72-73 0.8402207427439876 1102 74-75 0.9124663294478204 1102 76-77 0.83202721204702 1102 78-79 1.084620234534185 1102 80-81 1.111536605283419 1102 82-83 1.2469896297092866 1102 84-85 1.1212928974235261 1102 86-87 0.9039255217785396 1102 88-89 1.0893766359576311 1102 90-91 1.2583590437804517 1102 92-93 1.5546507502279248 1102 94-95 1.4532328572550384 1102 96-97 1.2727708209099653 1102 98-99 1.6875893071646857 1102 100-101 1.9860756503170407 1103 1 0.2202287279319819 1103 2 0.17858350206528684 1103 3 0.23637612777449846 1103 4 0.10683909363068267 1103 5 0.07516545971657962 1103 6 0.07796415940554624 1103 7 0.17138728397874559 1103 8 0.13191752248937405 1103 9 0.20908615878747838 1103 10-11 0.22192144899879196 1103 12-13 0.3305977779251208 1103 14-15 0.41914922304414404 1103 16-17 0.36439122452776473 1103 18-19 0.39572933200042826 1103 20-21 0.3627332105705534 1103 22-23 0.3251220285439942 1103 24-25 0.36665678134099267 1103 26-27 0.43320306146600274 1103 28-29 0.40661085519275275 1103 30-31 0.28803847357561807 1103 32-33 0.4238195081515741 1103 34-35 0.4604810665822683 1103 36-37 0.5469947914745603 1103 38-39 0.48734252221214547 1103 40-41 0.4299318859949324 1103 42-43 0.5215133214742167 1103 44-45 0.544851728616564 1103 46-47 0.5672251604369407 1103 48-49 0.6831430387046993 1103 50-51 0.7711038666272785 1103 52-53 0.6403068885971166 1103 54-55 0.6297160089523146 1103 56-57 0.6857966375922615 1103 58-59 0.6843980227232365 1103 60-61 0.6790611515611005 1103 62-63 0.7899370460555133 1103 64-65 0.7857842685970908 1103 66-67 0.7902202569812857 1103 68-69 0.7822014238207302 1103 70-71 0.6124412505308001 1103 72-73 0.7229487915866777 1103 74-75 0.7311700559858032 1103 76-77 0.6454214836084553 1103 78-79 0.9455823932352914 1103 80-81 0.9877343114121828 1103 82-83 1.1144146290036225 1103 84-85 1.269440615709474 1103 86-87 1.2742886429528006 1103 88-89 1.3250704574801695 1103 90-91 1.4130258699019187 1103 92-93 1.6336152633633851 1103 94-95 1.289490725383935 1103 96-97 1.3524410267799816 1103 98-99 1.8365299974059042 1103 100-101 1.8676707405947042 1104 1 0.2068276567964915 1104 2 0.25297846888024367 1104 3 0.22172899781293864 1104 4 0.18494200326082222 1104 5 0.10049695023617033 1104 6 0.13651200777652406 1104 7 0.20568269566045672 1104 8 0.16287679342618588 1104 9 0.2826795736077301 1104 10-11 0.3000015034193879 1104 12-13 0.2629452778891235 1104 14-15 0.4264634299863701 1104 16-17 0.43607200023717496 1104 18-19 0.3713536211494244 1104 20-21 0.30934542065131865 1104 22-23 0.37675158698854005 1104 24-25 0.3736310395364768 1104 26-27 0.3016218408898368 1104 28-29 0.3355363724043414 1104 30-31 0.2917197819733772 1104 32-33 0.353741656469289 1104 34-35 0.4050240703686754 1104 36-37 0.3548060542338831 1104 38-39 0.20954374883504556 1104 40-41 0.25960658617776744 1104 42-43 0.30464599686545313 1104 44-45 0.2496196492604028 1104 46-47 0.23038436226823933 1104 48-49 0.36104457054362626 1104 50-51 0.44384983907145426 1104 52-53 0.3667732212537871 1104 54-55 0.3503166510577387 1104 56-57 0.27274037418506936 1104 58-59 0.36941814250612737 1104 60-61 0.5314211768997836 1104 62-63 0.4567305861295168 1104 64-65 0.26084905501785727 1104 66-67 0.3578443229770514 1104 68-69 0.48503151063453487 1104 70-71 0.49570241898988954 1104 72-73 0.3605947409956798 1104 74-75 0.44288200116209353 1104 76-77 0.5078084672966732 1104 78-79 0.6670234591878668 1104 80-81 0.7921433872698813 1104 82-83 0.9980612766662738 1104 84-85 0.9114861029753456 1104 86-87 1.005407000545052 1104 88-89 0.8799816280988075 1104 90-91 1.0076032464837894 1104 92-93 1.2235357560281237 1104 94-95 1.309965393893414 1104 96-97 1.4062360713386433 1104 98-99 1.6314953726632488 1104 100-101 1.7186680915499437 1105 1 0.1570877546366738 1105 2 0.21206312422692974 1105 3 0.2069642912215599 1105 4 0.14357706214229182 1105 5 0.11334084159602753 1105 6 0.15783796435284358 1105 7 0.16027620776478102 1105 8 0.19609406853755473 1105 9 0.25693031791286813 1105 10-11 0.22566251009531157 1105 12-13 0.2770912081887573 1105 14-15 0.34273241643992947 1105 16-17 0.29001686052821896 1105 18-19 0.31641641140259935 1105 20-21 0.32409919297401757 1105 22-23 0.297226909118983 1105 24-25 0.33572726798864494 1105 26-27 0.2234939082242633 1105 28-29 0.27742572793682285 1105 30-31 0.35850767265824146 1105 32-33 0.4525249392639168 1105 34-35 0.5424301174497472 1105 36-37 0.45544885147810277 1105 38-39 0.4390747117566747 1105 40-41 0.5365836655398937 1105 42-43 0.5327803006797112 1105 44-45 0.541206547423613 1105 46-47 0.5420114503261217 1105 48-49 0.7093309898375324 1105 50-51 0.708328877131315 1105 52-53 0.7073545971808315 1105 54-55 0.7447757319248325 1105 56-57 0.7166629871136863 1105 58-59 0.7885420062462032 1105 60-61 0.8061965078120892 1105 62-63 0.6774538664789986 1105 64-65 0.8020681484894965 1105 66-67 0.7087483373173029 1105 68-69 0.7967296283905796 1105 70-71 0.7747359157345777 1105 72-73 0.7333052170582448 1105 74-75 0.7990949042879194 1105 76-77 0.8104307976562275 1105 78-79 0.9461458076277083 1105 80-81 0.9886241885881937 1105 82-83 1.1938758624641217 1105 84-85 1.3507913550403714 1105 86-87 1.2179950127983155 1105 88-89 1.3111557447046032 1105 90-91 1.506791355509641 1105 92-93 1.5512840887673889 1105 94-95 1.5153328283695942 1105 96-97 1.6092030464033407 1105 98-99 1.5967691684730987 1105 100-101 1.358300682902744 1106 1 -0.022605705523112363 1106 2 0.12212126579188975 1106 3 0.0639655082172581 1106 4 0.007883319237528497 1106 5 0.023871303534896526 1106 6 0.02256697628689608 1106 7 0.08516568598957974 1106 8 0.09865112213049798 1106 9 -0.07799132681125087 1106 10-11 0.10202571134757221 1106 12-13 0.12237196458267618 1106 14-15 0.03973387189525113 1106 16-17 0.05382916555554118 1106 18-19 0.055034350549412636 1106 20-21 0.042931285891512516 1106 22-23 0.15776030922101114 1106 24-25 0.19103729530078795 1106 26-27 0.11293483113112046 1106 28-29 0.2103301746125723 1106 30-31 0.18272086237075058 1106 32-33 0.19679505269404984 1106 34-35 0.2447686398347173 1106 36-37 0.21942347937464035 1106 38-39 0.08163524004693556 1106 40-41 0.21555535953147853 1106 42-43 0.22195167905295676 1106 44-45 0.12415608851659954 1106 46-47 -0.3432069488774161 1106 48-49 -0.05318153993798802 1106 50-51 0.3929187649105188 1106 52-53 0.28836316213936897 1106 54-55 0.48081328250169264 1106 56-57 0.40578164887829615 1106 58-59 0.2534216735609718 1106 60-61 0.2741552849570894 1106 62-63 0.11116592478578013 1106 64-65 0.26150344204606313 1106 66-67 0.15395006138034972 1106 68-69 0.07120857283204174 1106 70-71 0.19969505823378952 1106 72-73 0.5008057259140344 1106 74-75 0.5415991390752595 1106 76-77 0.46599972398327694 1106 78-79 0.7830814141625275 1106 80-81 0.16225634588978366 1106 82-83 0.5479751962249786 1106 84-85 0.8362627769324966 1106 86-87 0.9577166793244629 1106 88-89 1.0707196361271265 1106 90-91 1.0054784649787862 1106 92-93 1.1019387881677467 1106 94-95 1.2724694563632255 1106 96-97 1.37937941284083 1106 98-99 1.296392998424139 1106 100-101 1.15957167267198 1107 1 0.10154095230157267 1107 2 0.13174270880989525 1107 3 0.0828076937301887 1107 4 0.04023400192780713 1107 5 0.006456293223841669 1107 6 0.053455810418704175 1107 7 0.05374330111311565 1107 8 0.09212012806355574 1107 9 0.12156376275790848 1107 10-11 0.08984783868202584 1107 12-13 0.15548601236354642 1107 14-15 0.2515919669112634 1107 16-17 0.22098288538930433 1107 18-19 0.1484392741660443 1107 20-21 0.17738946370464248 1107 22-23 0.21550487076842728 1107 24-25 0.22683858334157492 1107 26-27 0.23051888094609296 1107 28-29 0.191611964264915 1107 30-31 0.1814333728375388 1107 32-33 0.17749610496702672 1107 34-35 0.2231515384281053 1107 36-37 0.24352322618747735 1107 38-39 0.2241453540280105 1107 40-41 0.18007836935522903 1107 42-43 0.23167408272689727 1107 44-45 0.23398072071167064 1107 46-47 0.3007940643122353 1107 48-49 0.3282557010109741 1107 50-51 0.3678643707378413 1107 52-53 0.5130896477879574 1107 54-55 0.27940077043629685 1107 56-57 0.2724850836353383 1107 58-59 0.31074952089677055 1107 60-61 0.2592214316887862 1107 62-63 0.513341168009184 1107 64-65 0.26527241936946666 1107 66-67 0.3056162567319127 1107 68-69 0.5455614516853231 1107 70-71 0.6015577175644786 1107 72-73 0.5783156166405945 1107 74-75 0.5206722248882336 1107 76-77 0.5176464879227645 1107 78-79 0.8596280580958222 1107 80-81 0.8081165731242983 1107 82-83 1.0188071307722701 1107 84-85 1.0279667491541211 1107 86-87 1.1928466051874231 1107 88-89 1.1328965992372098 1107 90-91 1.3203146650655526 1107 92-93 1.4189077579942122 1107 94-95 1.4350039298932273 1107 96-97 1.522239071740998 1107 98-99 1.5642423903725344 1107 100-101 1.7560749280536498 1108 1 0.1572424782309163 1108 2 0.08229061920621916 1108 3 0.12222332165716665 1108 4 0.09589481879625339 1108 5 0.04012284898718832 1108 6 0.1314780819561534 1108 7 0.12789426005510762 1108 8 0.16775729253455296 1108 9 0.2309970265862944 1108 10-11 0.1938179411248626 1108 12-13 0.2484284253909692 1108 14-15 0.3715343668381621 1108 16-17 0.31874225759496255 1108 18-19 0.3353516521349533 1108 20-21 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2316 68-69 -0.9357846086289143 2316 70-71 -0.9376326798890737 2316 72-73 -1.0711736463213128 2316 74-75 -1.0729274510647606 2316 76-77 -1.2902884426621846 2316 78-79 -1.3398720053776323 2316 80-81 -1.3001918747118673 2316 82-83 -1.3064582679238406 2316 84-85 -1.4464126118755836 2316 86-87 -1.66203986914255 2316 88-89 -1.869065986144605 2316 90-91 -1.8099931901406912 2316 92-93 -1.8769499371671294 2316 94-95 -1.7112088789119682 2316 96-97 -1.9291917876262126 2316 98-99 -1.7844453184553828 2316 100-101 -2.0097971280418925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 29.0 4 68.0 5 130.0 6 270.0 7 671.0 8 1615.0 9 2789.0 10 4595.0 11 6880.0 12 9864.0 13 13945.0 14 18266.0 15 23717.0 16 28978.0 17 35344.0 18 36181.0 19 35178.0 20 37083.0 21 40175.0 22 44640.0 23 50318.0 24 58214.0 25 68327.0 26 80969.0 27 95998.0 28 115005.0 29 135523.0 30 160455.0 31 189759.0 32 227361.0 33 277551.0 34 350137.0 35 448577.0 36 580672.0 37 620668.0 38 265477.0 39 8443.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26047856788507 18.777803770033504 14.548795578054538 24.41292208402688 2 16.392103545309915 19.9914435918053 38.66135927041639 24.955093592468398 3 19.852504210360692 22.913419863476552 28.887971390411938 28.346104535750815 4 13.927343921442912 16.19982238054257 34.984945548197565 34.88788814981695 5 14.696739749710222 36.368146008919275 33.738703561816074 15.196410679554429 6 34.58434445017745 34.69428883240981 16.412813540317114 14.308553177095632 7 30.503532382773273 30.971661161266695 19.691208305401315 18.833598150558718 8 26.800575078444894 35.43166107780815 18.881316799050733 18.886447044696222 9 26.343099537320576 16.670524578663233 19.13417163347267 37.85220425054352 10-11 25.783706388923182 25.851970566627042 26.724897818516457 21.639425225933316 12-13 26.284776744719224 22.768103814570832 27.171634209067086 23.775485231642865 14-15 23.054133419279268 24.55027505726115 24.825823251009666 27.569768272449917 16-17 23.37789119411745 27.70206233420286 25.560184777211102 23.359861694468588 18-19 23.331559430213808 26.610682349672143 27.208650526930228 22.849107693183818 20-21 23.66729596043476 25.866698544556677 27.421739820942154 23.04426567406641 22-23 23.577185282135282 25.575771887186633 27.27713562430303 23.569907206375053 24-25 23.558926620944042 25.875919007202803 27.22454700320096 23.340607368652194 26-27 23.196865827442544 26.195366613869965 27.255985041002845 23.351782517684644 28-29 23.24573612242303 26.333067738472028 27.25909048733466 23.162105651770283 30-31 23.348094290111714 25.988129048728343 27.14467537759493 23.519101283565018 32-33 23.2375485933846 25.911636073627527 27.234289274267603 23.616526058720275 34-35 23.70278191471276 26.055853348045044 26.990416726864137 23.250948010378064 36-37 23.80035063033543 25.69109298038363 27.029459356306933 23.479097032974007 38-39 23.243834112640485 25.989946741343996 27.117115038304206 23.64910410771131 40-41 23.64434472944089 25.86687106060448 27.046926941645243 23.441857268309388 42-43 23.509889679643234 25.768704660923074 26.91490229986984 23.806503359563848 44-45 23.39067494120593 26.044374180688052 27.091780122448778 23.47317075565724 46-47 23.595570707917442 26.247748153541135 26.606853075410434 23.549828063130988 48-49 23.609623653525304 25.999029426254438 26.79787494915779 23.593471971062467 50-51 23.654326090964055 26.23369141384896 26.63076042790961 23.481222067277375 52-53 23.713652270968282 26.127727406729555 26.520768044411724 23.637852277890435 54-55 23.45998048019889 26.0983815649814 26.71976517325787 23.72187278156184 56-57 23.491075704506688 26.319927518711282 26.671864824654833 23.517131952127198 58-59 23.52091526600569 25.98185397443332 26.7406022846295 23.75662847493149 60-61 23.470677455074142 26.161994502045836 26.723584573817732 23.643743469062287 62-63 23.25885231085761 26.270121371101524 26.75625141386452 23.714774904176352 64-65 23.167196750510342 26.14844884496551 26.72124627601267 23.96310812851147 66-67 23.223642325068223 26.186211515855902 26.64496928338369 23.945176875692184 68-69 23.19909614628505 26.000254589391137 26.816290922267864 23.984358342055945 70-71 23.251277960173052 26.061624374827407 26.64309778773281 24.04399987726673 72-73 23.29640496063026 26.00613297773436 26.600701665673547 24.096760395961834 74-75 23.151732104735284 26.34684815740983 26.321215536216513 24.180204201638375 76-77 23.333647283266792 26.115407422488644 26.514766473438055 24.036178820806505 78-79 23.319872333095855 26.073032177368454 26.644014634770976 23.96308085476472 80-81 23.16931914232767 26.314735618961798 26.50984864714227 24.006096591568262 82-83 23.26646795435551 26.29262964112075 26.386014686965492 24.054887717558245 84-85 23.216909111085144 26.159409864785637 26.386489759002423 24.237191265126793 86-87 23.27734633484355 26.051721680908603 26.461985594365302 24.208946389882545 88-89 23.141246199674477 26.1759665878451 26.307735773730922 24.3750514387495 90-91 23.15581951382638 26.119175047742793 26.442927412785 24.282078025645824 92-93 23.07286716490579 25.868655007258994 26.521891036729073 24.536586791106142 94-95 22.94568985911236 25.915770893353184 26.50884180098407 24.629697446550388 96-97 22.977256676544748 25.815678351090632 26.428609665851077 24.778455306513543 98-99 22.908334513224997 25.857826267470735 26.274052751656768 24.959786467647493 100-101 23.008313039588668 25.606783886134572 26.501316684422545 24.88358638985422 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 288.0 1 211.0 2 205.5 3 289.5 4 251.5 5 239.5 6 450.5 7 1007.0 8 1610.0 9 1781.5 10 1589.0 11 1444.0 12 1475.0 13 1581.0 14 1788.5 15 2015.0 16 2191.0 17 2391.5 18 2625.0 19 2879.5 20 3273.5 21 3934.5 22 4670.5 23 5680.0 24 7267.5 25 9409.0 26 12362.5 27 15838.0 28 19474.5 29 23876.0 30 29201.0 31 36126.5 32 45913.5 33 53268.5 34 58463.0 35 64839.0 36 73920.5 37 83530.5 38 90268.0 39 96798.5 40 103788.0 41 114396.0 42 124214.0 43 132219.5 44 140789.5 45 149465.0 46 156310.5 47 163159.0 48 167114.0 49 166813.5 50 167547.5 51 164982.5 52 163640.5 53 166385.0 54 168656.0 55 159172.5 56 141439.5 57 127532.0 58 116188.5 59 102256.5 60 84523.5 61 70371.0 62 58747.0 63 46495.0 64 36310.5 65 28033.0 66 20982.5 67 16629.5 68 13121.5 69 9706.0 70 7385.0 71 5384.0 72 4163.0 73 3394.5 74 2652.5 75 2107.0 76 1468.0 77 850.0 78 502.5 79 324.0 80 188.0 81 91.0 82 45.0 83 23.5 84 13.0 85 7.0 86 3.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.927460781235771E-4 2 0.050983350266416844 3 0.0 4 2.4546629882723567E-5 5 0.044159387159019695 6 2.4546629882723567E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006320757194801318 26-27 0.0018778171860283528 28-29 1.2273314941361782E-4 30-31 0.0016937174619079262 32-33 5.400258574199185E-4 34-35 0.01177010902876595 36-37 0.022791545846108832 38-39 0.004295660229476624 40-41 0.0011414182895466457 42-43 0.0015096177377874993 44-45 0.008321307530243289 46-47 0.0 48-49 0.0 50-51 6.873056367162598E-4 52-53 0.0 54-55 0.001644624202142479 56-57 0.0 58-59 0.022607446121988405 60-61 0.0 62-63 0.009339992670376317 64-65 0.015317097046819507 66-67 0.006615316753394001 68-69 0.01604122262835985 70-71 9.818651953089427E-5 72-73 3.681994482408535E-5 74-75 0.023172018609291047 76-77 0.05165838258819175 78-79 0.030671014038463097 80-81 0.027283579114647242 82-83 0.036635845099964925 84-85 0.015783483014591253 86-87 0.009904565157678959 88-89 0.08601139110906338 90-91 0.024865736071198973 92-93 0.024595723142489014 94-95 0.026866286406640943 96-97 0.03836638250669693 98-99 0.053450286569630565 100-101 0.014224772017038307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4073879.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.64147887317476 #Duplication Level Percentage of deduplicated Percentage of total 1 70.86425798248807 32.34349533570935 2 14.945278614241447 13.642492362512233 3 5.675004582000663 7.770468052036596 4 2.661946709574795 4.859807380263003 5 1.5270312986532875 3.484798337808033 6 0.9624978184062062 2.6357894306558194 7 0.6206383482128047 1.9828796440485765 8 0.4397812027572206 1.6057811579570456 9 0.3208675294618932 1.31803817103205 >10 1.7363243011611402 14.416193277955133 >50 0.14514349738278884 4.560334685067308 >100 0.09347240028442123 7.8951875154495195 >500 0.005870645720199337 1.8356441481950017 >1k 0.0017773513891799714 1.3559489014276687 >5k 1.0771826601090678E-4 0.2931415998826123 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6719 0.16492880618201963 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5206 0.1277897551694589 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.4546629882723567E-5 7 0.0 0.0 0.0 0.0 2.4546629882723567E-5 8 0.0 0.0 0.0 0.0 7.36398896481707E-5 9 0.0 0.0 0.0 2.4546629882723567E-5 7.36398896481707E-5 10-11 0.0 0.0 0.0 2.4546629882723567E-5 7.36398896481707E-5 12-13 0.0 0.0 0.0 2.4546629882723567E-5 7.36398896481707E-5 14-15 0.0 0.0 0.0 4.9093259765447134E-5 7.36398896481707E-5 16-17 0.0 0.0 0.0 4.9093259765447134E-5 7.36398896481707E-5 18-19 0.0 0.0 0.0 4.9093259765447134E-5 7.36398896481707E-5 20-21 0.0 0.0 0.0 8.591320458953248E-5 7.36398896481707E-5 22-23 0.0 0.0 0.0 1.9637303906178854E-4 9.818651953089427E-5 24-25 0.0 0.0 0.0 4.0501939306493884E-4 9.818651953089427E-5 26-27 0.0 0.0 0.0 7.486722114230688E-4 9.818651953089427E-5 28-29 0.0 0.0 0.0 0.002258289949210568 1.1045983447225605E-4 30-31 0.0 0.0 0.0 0.00894724659225274 1.2273314941361782E-4 32-33 0.0 0.0 0.0 0.02477982286660944 1.2273314941361782E-4 34-35 0.0 0.0 0.0 0.047203169264477415 1.2273314941361782E-4 36-37 0.0 0.0 0.0 0.08745964227214406 1.472797792963414E-4 38-39 0.0 0.0 0.0 0.15144043306146304 1.472797792963414E-4 40-41 0.0 0.0 0.0 0.22182789425017285 1.472797792963414E-4 42-43 0.0 0.0 0.0 0.29331995378360526 1.472797792963414E-4 44-45 0.0 0.0 0.0 0.3809268758350457 1.472797792963414E-4 46-47 0.0 0.0 0.0 0.4873119697467696 1.472797792963414E-4 48-49 0.0 1.2273314941361784E-5 0.0 0.6036016288161725 1.472797792963414E-4 50-51 0.0 2.4546629882723567E-5 0.0 0.7276848428733401 1.472797792963414E-4 52-53 0.0 2.4546629882723567E-5 0.0 0.8523939959925172 1.7182640917906496E-4 54-55 0.0 2.4546629882723567E-5 0.0 0.9898183033909451 1.9637303906178854E-4 56-57 0.0 2.4546629882723567E-5 0.0 1.1522065333801028 1.9637303906178854E-4 58-59 0.0 2.4546629882723567E-5 0.0 1.3285249758277062 1.9637303906178854E-4 60-61 0.0 2.4546629882723567E-5 0.0 1.5190927369222307 2.209196689445121E-4 62-63 0.0 2.4546629882723567E-5 0.0 1.7080895137042607 2.209196689445121E-4 64-65 0.0 2.4546629882723567E-5 0.0 1.9097032582460107 2.209196689445121E-4 66-67 0.0 2.4546629882723567E-5 0.0 2.1433994480444802 2.209196689445121E-4 68-69 0.0 2.4546629882723567E-5 0.0 2.3999117303189417 2.209196689445121E-4 70-71 0.0 2.4546629882723567E-5 0.0 2.674367599037674 2.5773961376859746E-4 72-73 0.0 2.4546629882723567E-5 0.0 2.9560279036269854 3.681994482408535E-4 74-75 0.0 2.4546629882723567E-5 0.0 3.245359030054648 3.927460781235771E-4 76-77 0.0 2.4546629882723567E-5 0.0 3.5652507106863016 3.927460781235771E-4 78-79 0.0 3.681994482408535E-5 0.0 3.9199617858065983 3.927460781235771E-4 80-81 0.0 4.9093259765447134E-5 0.0 4.29616343538922 3.927460781235771E-4 82-83 0.0 4.9093259765447134E-5 0.0 4.683816087812132 4.6638596777174776E-4 84-85 0.0 4.9093259765447134E-5 0.0 5.074291602671557 4.6638596777174776E-4 86-87 0.0 4.9093259765447134E-5 0.0 5.48638042514272 4.6638596777174776E-4 88-89 0.0 4.9093259765447134E-5 0.0 5.932208590387687 4.6638596777174776E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5120 0.0 43.0697 1 GTATCAA 8150 0.0 40.236046 1 ATCAACG 10235 0.0 31.420753 3 TCAACGC 10545 0.0 30.587149 4 TATCAAC 10770 0.0 30.006617 2 CAACGCA 10840 0.0 29.710924 5 AACGCAG 11470 0.0 28.108707 6 TACATGG 11610 0.0 24.560816 2 GTACATG 11980 0.0 23.921272 1 CGCAGAG 13570 0.0 23.513859 8 ACGCAGA 13870 0.0 23.17644 7 ACATGGG 12020 0.0 22.684187 3 GCAGAGT 15295 0.0 20.334164 9 GAGTACT 7760 0.0 19.243914 12-13 CATGGGG 8395 0.0 19.012285 4 GGACCGA 1550 0.0 18.686703 6 AGAGTAC 13695 0.0 18.65326 10-11 AGGACCG 1830 0.0 18.170326 5 AGTACTT 8235 0.0 18.047424 12-13 GTACTTT 8290 0.0 17.72722 14-15 >>END_MODULE