FastQCFastQC Report
Thu 26 May 2016
SRR938390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4011289
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63460.1582035101434975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48930.12198073985693876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.042.723741
GTATCAA82950.040.4015541
ATCAACG101350.032.4499243
TATCAAC106150.031.2512052
TCAACGC105150.031.1416244
CAACGCA105900.030.830555
AACGCAG113600.028.943126
GTACATG115350.025.2153741
TACATGG114600.024.5509572
CGCAGAG134400.024.072558
ACGCAGA134950.024.0448477
ACATGGG119550.022.5008143
GCAGAGT149350.020.5479769
GAGTACT75600.020.23107312-13
TAGGTCG2851.9554136E-920.0104545
CATGGGG86200.019.6830464
GTGTAAG16750.019.6099321
GTACTTT79000.019.06164414-15
TAAGGTG14850.018.8819165
AGAGTAC129750.018.87301810-11
Produced by FastQC (version 0.11.5)