##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938390_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4011289 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.976712722518872 31.0 31.0 34.0 28.0 34.0 2 31.07771990499812 31.0 31.0 34.0 27.0 34.0 3 31.153489813374204 31.0 31.0 34.0 27.0 34.0 4 34.77500175130737 37.0 35.0 37.0 32.0 37.0 5 34.77903237587718 37.0 35.0 37.0 32.0 37.0 6 34.79972996211442 37.0 35.0 37.0 32.0 37.0 7 34.71341331925972 37.0 35.0 37.0 31.0 37.0 8 34.67212310057939 37.0 35.0 37.0 30.0 37.0 9 36.17047786883468 39.0 35.0 39.0 31.0 39.0 10-11 36.01507296033769 39.0 35.0 39.0 30.0 39.0 12-13 35.85316228773344 38.0 35.0 39.0 30.0 39.0 14-15 36.84313147220257 39.0 36.0 41.0 30.5 41.0 16-17 36.67630093468708 39.0 36.0 41.0 30.0 41.0 18-19 36.63235196965364 39.0 36.0 40.5 30.0 41.0 20-21 36.71787697171658 39.0 36.0 41.0 30.0 41.0 22-23 36.633214909222445 39.0 36.0 41.0 30.0 41.0 24-25 36.50987675034135 39.0 36.0 41.0 29.5 41.0 26-27 36.31453355766688 39.0 35.5 41.0 29.0 41.0 28-29 36.12766257928561 39.0 35.0 40.5 28.5 41.0 30-31 35.92949984406509 39.0 35.0 40.0 27.0 41.0 32-33 35.67120032986902 38.0 34.5 40.0 27.0 41.0 34-35 35.423181426219855 38.0 34.0 40.0 26.5 41.0 36-37 35.1414767173345 38.0 34.0 40.0 25.0 41.0 38-39 34.89635239445475 38.0 34.0 40.0 25.0 41.0 40-41 34.85871947895053 38.0 34.0 40.0 25.0 41.0 42-43 34.69153531445877 38.0 33.0 40.0 24.0 41.0 44-45 34.431462554804696 38.0 33.0 40.0 24.0 41.0 46-47 34.09706455954682 38.0 33.0 40.0 23.0 41.0 48-49 33.75783769257214 38.0 33.0 40.0 21.5 41.0 50-51 33.021479753764936 37.0 31.5 39.5 20.0 40.5 52-53 33.08212921083472 37.0 32.0 39.5 20.0 40.0 54-55 33.20439315142838 37.0 32.0 40.0 19.5 41.0 56-57 32.881177970472834 37.0 31.5 40.0 17.5 41.0 58-59 32.52879834885993 36.5 31.0 39.5 14.5 41.0 60-61 32.05780598206711 36.0 31.0 39.0 11.5 41.0 62-63 31.508737839632097 35.5 30.0 39.0 8.0 40.0 64-65 30.99294752883674 35.0 29.0 38.5 7.0 40.0 66-67 30.424963147756245 35.0 29.0 38.0 2.0 40.0 68-69 29.831632176091027 34.0 28.0 37.5 2.0 39.5 70-71 29.186319160748578 34.0 27.5 37.0 2.0 39.0 72-73 28.603252096769893 34.0 26.0 36.0 2.0 39.0 74-75 27.949119472568544 33.0 26.0 36.0 2.0 38.5 76-77 27.180081888889084 32.5 24.5 35.0 2.0 37.5 78-79 26.37586010382199 32.0 22.5 35.0 2.0 37.0 80-81 25.700893528239924 31.5 20.5 35.0 2.0 36.0 82-83 25.023335765635437 31.0 18.5 34.5 2.0 36.0 84-85 24.33881303491222 31.0 15.0 34.0 2.0 35.0 86-87 23.63876948282709 30.5 7.5 34.0 2.0 35.0 88-89 22.8285202088406 29.5 2.0 34.0 2.0 35.0 90-91 21.974125150294583 29.0 2.0 34.0 2.0 35.0 92-93 21.09055567923428 29.0 2.0 34.0 2.0 35.0 94-95 20.092642040999788 27.0 2.0 33.5 2.0 35.0 96-97 18.82632677426134 25.5 2.0 33.0 2.0 34.5 98-99 17.335937276022747 21.5 2.0 32.5 2.0 34.0 100-101 13.974898717095677 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7369676321366896 1101 2 0.6716214146629937 1101 3 0.7715999909111098 1101 4 0.660079797494511 1101 5 0.6641813287778007 1101 6 0.6249538375736279 1101 7 0.6386293540214112 1101 8 0.6539331187072861 1101 9 0.5603882732405268 1101 10-11 0.7289220843270456 1101 12-13 0.7744809756576458 1101 14-15 0.9929548795231256 1101 16-17 0.9569352962501441 1101 18-19 0.9012602550344866 1101 20-21 0.9607477054196849 1101 22-23 0.9385665958427651 1101 24-25 1.0181858340906942 1101 26-27 1.0981623610161861 1101 28-29 0.9830669696333203 1101 30-31 0.9655592062272476 1101 32-33 1.048293892478334 1101 34-35 1.058786048294671 1101 36-37 1.0925825393440292 1101 38-39 1.0286878154923045 1101 40-41 0.9759853698107719 1101 42-43 1.1542447092160089 1101 44-45 1.0260512258403978 1101 46-47 0.956614467046343 1101 48-49 1.0889571058462195 1101 50-51 1.0309118402423465 1101 52-53 1.1910928262653968 1101 54-55 1.2298598486854146 1101 56-57 1.1325140359902903 1101 58-59 1.115318629845639 1101 60-61 1.1146015468806354 1101 62-63 1.0795976210795857 1101 64-65 1.0569767470754172 1101 66-67 1.0975494360961733 1101 68-69 1.0965762354087083 1101 70-71 1.2504592173533773 1101 72-73 1.256067217699222 1101 74-75 1.0560261705015712 1101 76-77 1.2239987042905014 1101 78-79 1.1731943830470257 1101 80-81 1.134902292898797 1101 82-83 1.2008336343503103 1101 84-85 1.47997004663468 1101 86-87 1.5497491563939185 1101 88-89 1.6632104312432467 1101 90-91 1.573226699342662 1101 92-93 1.5165672402839405 1101 94-95 1.3752093504872853 1101 96-97 1.4057945805727385 1101 98-99 1.490059484168917 1101 100-101 1.2760819023194045 1102 1 0.5483654202311001 1102 2 0.6332467082620497 1102 3 0.5325291954548454 1102 4 0.5628521207462569 1102 5 0.6155195138332559 1102 6 0.656326733221988 1102 7 0.4694142682699578 1102 8 0.5430055482705285 1102 9 0.6806386940568387 1102 10-11 0.6964668629096025 1102 12-13 0.8213542537680567 1102 14-15 0.7353999708278423 1102 16-17 0.7728407549237133 1102 18-19 0.8721795658032789 1102 20-21 0.7062721430167826 1102 22-23 0.8283106636293027 1102 24-25 0.6141523448914725 1102 26-27 0.6802237694057212 1102 28-29 0.6117804102036857 1102 30-31 0.6561809582708307 1102 32-33 0.6561279701181277 1102 34-35 0.7007740758276171 1102 36-37 0.6434885503727585 1102 38-39 0.6844158803747149 1102 40-41 0.8421674719908054 1102 42-43 0.8231519143582844 1102 44-45 0.8773830095181196 1102 46-47 0.7806986311182484 1102 48-49 0.716990703895874 1102 50-51 0.8401533161461572 1102 52-53 0.8545615951048902 1102 54-55 0.9102757586210046 1102 56-57 0.9254435792398326 1102 58-59 1.028779802113462 1102 60-61 1.030951543423349 1102 62-63 0.7776801856173563 1102 64-65 0.8632064355308984 1102 66-67 0.9499074322002485 1102 68-69 0.8305447344179804 1102 70-71 0.8642442804272044 1102 72-73 0.8821040315633653 1102 74-75 0.8012296951487663 1102 76-77 0.9000897002800556 1102 78-79 0.8048865575383175 1102 80-81 0.8590143245718416 1102 82-83 1.1032630652381208 1102 84-85 1.0215759294738014 1102 86-87 0.9142125764366575 1102 88-89 1.1134139248289046 1102 90-91 1.4006099025596654 1102 92-93 1.1743562719418605 1102 94-95 1.1026021220083386 1102 96-97 1.1111380158383284 1102 98-99 1.1066505857676923 1102 100-101 0.9770895521008391 1103 1 0.5442109590662518 1103 2 0.6130329243842176 1103 3 0.5205192217374304 1103 4 0.5679268973488334 1103 5 0.5422133137140932 1103 6 0.5358905722220726 1103 7 0.5526737473275602 1103 8 0.46606700897800835 1103 9 0.46309744547868803 1103 10-11 0.5644568139266752 1103 12-13 0.5963454992869259 1103 14-15 0.7339656763475304 1103 16-17 0.8379963173656293 1103 18-19 0.7642733917804847 1103 20-21 0.742741653642419 1103 22-23 0.7541252521213195 1103 24-25 0.8134809960616209 1103 26-27 0.808050451377234 1103 28-29 0.7072745889035872 1103 30-31 0.702643716412851 1103 32-33 0.8828603695233497 1103 34-35 0.9675335461583146 1103 36-37 0.9161305713501662 1103 38-39 0.7928445332513121 1103 40-41 0.9832290413141962 1103 42-43 0.8419358483135824 1103 44-45 1.0442194128729696 1103 46-47 0.9504971213571238 1103 48-49 0.9319804194487205 1103 50-51 0.9980763697558999 1103 52-53 0.9491407464175623 1103 54-55 0.9852229089360023 1103 56-57 1.1049316271751408 1103 58-59 1.0409091300502595 1103 60-61 1.0359440146020162 1103 62-63 1.2221825174529783 1103 64-65 1.108938021744251 1103 66-67 1.1970956654508953 1103 68-69 1.188439991698509 1103 70-71 1.2595045131779905 1103 72-73 1.1986670102657584 1103 74-75 1.160099835357368 1103 76-77 1.0694994377027172 1103 78-79 1.0283386786532276 1103 80-81 1.1583011774336889 1103 82-83 1.0954606278426375 1103 84-85 1.1726390190306013 1103 86-87 1.2321406832166097 1103 88-89 1.2806716852376532 1103 90-91 1.2658774159502713 1103 92-93 1.4322500823079558 1103 94-95 1.3295095461522273 1103 96-97 1.3811125559839468 1103 98-99 1.4754101914465316 1103 100-101 1.049840734012566 1104 1 0.5118706980828343 1104 2 0.5269384308499987 1104 3 0.5321883708058408 1104 4 0.5746014689949703 1104 5 0.5119334089609069 1104 6 0.5108151511255983 1104 7 0.48195897938187926 1104 8 0.41014448968635975 1104 9 0.4889788097501153 1104 10-11 0.5381186058560061 1104 12-13 0.5802867419169502 1104 14-15 0.7163589535421124 1104 16-17 0.6211836467702838 1104 18-19 0.7720717433466291 1104 20-21 0.7984438458227459 1104 22-23 0.921633218509534 1104 24-25 0.8343969055399256 1104 26-27 0.7657123824997072 1104 28-29 0.7896082624041156 1104 30-31 0.8259124551964518 1104 32-33 0.6868348361419336 1104 34-35 0.7054464454918943 1104 36-37 0.7927084968066609 1104 38-39 0.7368614426127635 1104 40-41 0.7654574639581639 1104 42-43 0.7525544055134006 1104 44-45 0.7924897333968133 1104 46-47 0.8707742496909603 1104 48-49 0.7883328185206651 1104 50-51 0.605513749590159 1104 52-53 0.7249882428391103 1104 54-55 0.8667995987742358 1104 56-57 0.8690963901709026 1104 58-59 0.7770449907439811 1104 60-61 0.8748353970578719 1104 62-63 0.9276550409501922 1104 64-65 0.967105142091242 1104 66-67 1.163327093029153 1104 68-69 1.1227118924738981 1104 70-71 0.9069125619655587 1104 72-73 0.9718718839610077 1104 74-75 0.9994087041935096 1104 76-77 1.0894030334262865 1104 78-79 1.1276954128099632 1104 80-81 1.137467146273778 1104 82-83 1.2389965765183284 1104 84-85 1.2707759523302897 1104 86-87 1.3433825205065268 1104 88-89 1.3403874967597211 1104 90-91 1.1134556227704415 1104 92-93 1.2106013804886935 1104 94-95 1.1437519260720883 1104 96-97 1.174434374248321 1104 98-99 1.0663760727512503 1104 100-101 0.8523786083174372 1105 1 0.5035112440446206 1105 2 0.5056152011520716 1105 3 0.48987472256592923 1105 4 0.4698987479442138 1105 5 0.4369719568248769 1105 6 0.4674028236678254 1105 7 0.38990343731866517 1105 8 0.4303512870820043 1105 9 0.5349903071210065 1105 10-11 0.5166945386385677 1105 12-13 0.5299253838012561 1105 14-15 0.6455719024998672 1105 16-17 0.6625157861495197 1105 18-19 0.5736590111185365 1105 20-21 0.6179759313272868 1105 22-23 0.5743104546525402 1105 24-25 0.72481238215817 1105 26-27 0.7397315127500619 1105 28-29 0.6862283828505085 1105 30-31 0.6870725648205607 1105 32-33 0.6108288516955369 1105 34-35 0.6602106395563041 1105 36-37 0.9076568779383933 1105 38-39 0.8125737910428512 1105 40-41 0.6883923896721598 1105 42-43 0.8168531776153998 1105 44-45 0.8147642917119029 1105 46-47 0.7661441979324408 1105 48-49 0.7906078762892008 1105 50-51 0.8605211845819198 1105 52-53 0.8111112958583391 1105 54-55 0.713586870428152 1105 56-57 0.8977804037314172 1105 58-59 1.0672892802868432 1105 60-61 1.1726923867312635 1105 62-63 1.1966206610370236 1105 64-65 1.1562602917402742 1105 66-67 1.1679985804867172 1105 68-69 1.0840347268698132 1105 70-71 1.1108555575216066 1105 72-73 1.0340199882393115 1105 74-75 0.9778117811107627 1105 76-77 0.9410377256043745 1105 78-79 1.051891399287321 1105 80-81 0.9827978365279044 1105 82-83 1.1237998297355425 1105 84-85 0.8768316951217088 1105 86-87 0.9968633204273694 1105 88-89 1.0266846365247808 1105 90-91 1.0490149443469718 1105 92-93 1.051523802350271 1105 94-95 1.1943227114070005 1105 96-97 1.2962523705736686 1105 98-99 1.109227047679635 1105 100-101 0.7476003183039985 1106 1 0.3447259356785324 1106 2 0.4208472346750547 1106 3 0.1833637963598136 1106 4 0.33599719788596616 1106 5 0.38447113463401195 1106 6 0.019321715143469476 1106 7 0.24829876366623438 1106 8 0.21880685475571227 1106 9 0.35257937819095986 1106 10-11 0.13064936690062012 1106 12-13 0.1624167984070226 1106 14-15 0.447691816546282 1106 16-17 0.30005322398699263 1106 18-19 0.025383554243958883 1106 20-21 0.2145681167583504 1106 22-23 0.26878111644159475 1106 24-25 0.41033510746647295 1106 26-27 0.17424302289151683 1106 28-29 0.15783499863037065 1106 30-31 0.27444518714423083 1106 32-33 0.45976618253322243 1106 34-35 0.32235448999905003 1106 36-37 0.21462789495871704 1106 38-39 0.1471563592286742 1106 40-41 0.30285892783059865 1106 42-43 -0.10673038710466898 1106 44-45 0.19093258984815265 1106 46-47 -0.03526268884908035 1106 48-49 0.3493252455708813 1106 50-51 0.0531284004520316 1106 52-53 0.2538425036528835 1106 54-55 0.12299036963000987 1106 56-57 -0.20662227062640426 1106 58-59 -0.5817665828213592 1106 60-61 0.10311735180658133 1106 62-63 0.12576873923294585 1106 64-65 0.35533215646915295 1106 66-67 -0.1296586265925299 1106 68-69 -0.41680668480566396 1106 70-71 -0.37510422900238893 1106 72-73 0.1505294156937751 1106 74-75 0.22248281101964196 1106 76-77 0.1416712518179608 1106 78-79 0.21251577380024145 1106 80-81 0.15397933040005896 1106 82-83 0.24378412645016212 1106 84-85 0.20242343375734606 1106 86-87 0.35950691787470745 1106 88-89 0.22029560917922808 1106 90-91 0.32803707316154984 1106 92-93 0.3262763461893421 1106 94-95 0.22285491587728856 1106 96-97 0.15805756328235532 1106 98-99 0.08726399786271344 1106 100-101 0.047611721080119906 1107 1 0.45263957378756103 1107 2 0.3618218360121368 1107 3 0.3950696352692056 1107 4 0.4022040452962301 1107 5 0.3813779052298827 1107 6 0.45181136378861453 1107 7 0.3684278352550834 1107 8 0.3969088490556487 1107 9 0.42762650725514817 1107 10-11 0.4298172482604983 1107 12-13 0.41027411055038954 1107 14-15 0.47602863432371123 1107 16-17 0.5433780910419799 1107 18-19 0.5664138516101502 1107 20-21 0.5632071009839521 1107 22-23 0.6399103844123033 1107 24-25 0.5346887985934359 1107 26-27 0.5756936669327857 1107 28-29 0.6053718692811643 1107 30-31 0.6901693155555861 1107 32-33 0.6458824843100075 1107 34-35 0.7031031127873675 1107 36-37 0.6188594678947865 1107 38-39 0.6728271340798884 1107 40-41 0.6603547166359363 1107 42-43 0.752493166188394 1107 44-45 0.7504389605547956 1107 46-47 0.604207327978493 1107 48-49 0.6713334901666315 1107 50-51 0.5118108451359475 1107 52-53 0.4759791279399721 1107 54-55 0.5356355540835196 1107 56-57 0.621091116506264 1107 58-59 0.7366677184972588 1107 60-61 0.7534270964258241 1107 62-63 0.7081273490283948 1107 64-65 0.8030747031236132 1107 66-67 0.7606348257334048 1107 68-69 0.724949347654313 1107 70-71 0.6593231801907926 1107 72-73 0.5784182900677344 1107 74-75 0.7417781460985537 1107 76-77 0.8009301400763427 1107 78-79 0.7573296490648858 1107 80-81 0.6998278678371399 1107 82-83 0.7304084737730783 1107 84-85 0.7766516453106895 1107 86-87 0.6193959320050801 1107 88-89 0.7520315886591327 1107 90-91 0.8393921957590784 1107 92-93 0.9179876204528732 1107 94-95 0.8279633570442293 1107 96-97 0.9109976544234968 1107 98-99 0.792547151758793 1107 100-101 0.6770759875852761 1108 1 0.2711984471041866 1108 2 0.2547324420928341 1108 3 0.1686171940187684 1108 4 0.26614151463419233 1108 5 0.23038158246418305 1108 6 0.15947797741824843 1108 7 0.15394071441462387 1108 8 0.0829930917531101 1108 9 0.12300322488156468 1108 10-11 0.16158767414787434 1108 12-13 0.253540948703467 1108 14-15 0.37671300541499164 1108 16-17 0.2993410557237368 1108 18-19 0.20287056897138456 1108 20-21 0.283626054872677 1108 22-23 0.18883663310295162 1108 24-25 0.1661355328381191 1108 26-27 0.2526583575279986 1108 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-1.6084507396736143 2316 74-75 -1.4431472126542104 2316 76-77 -1.2292200431538802 2316 78-79 -1.3238969217279113 2316 80-81 -1.2995011118288637 2316 82-83 -1.109932972909732 2316 84-85 -1.1048800858583867 2316 86-87 -1.1469061479661349 2316 88-89 -1.283809048278922 2316 90-91 -1.3681532231461517 2316 92-93 -1.314264062685858 2316 94-95 -1.2839047149703724 2316 96-97 -1.4128849592469876 2316 98-99 -1.4597117072414232 2316 100-101 -0.9394192251432116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14708.0 3 5541.0 4 5973.0 5 6752.0 6 7765.0 7 9510.0 8 12020.0 9 14934.0 10 18275.0 11 22042.0 12 25978.0 13 29001.0 14 32844.0 15 36538.0 16 41183.0 17 46042.0 18 47149.0 19 47295.0 20 51656.0 21 56911.0 22 62134.0 23 69364.0 24 76867.0 25 86433.0 26 97061.0 27 109758.0 28 125381.0 29 142980.0 30 165255.0 31 192035.0 32 225648.0 33 269325.0 34 325579.0 35 396432.0 36 468274.0 37 453664.0 38 203294.0 39 9688.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38403679041447 17.94566705354246 13.911743865745857 24.75855229029721 2 15.84155724111682 19.208564387168284 39.61221116555888 25.337667206156016 3 19.698211090118907 22.557299363375048 28.445575113638487 29.298914432867555 4 13.408607133717418 15.486111145753775 35.27045140834075 35.83483031218806 5 14.83895541967527 36.23171920823583 33.5543653766188 15.374959995470103 6 34.5936089006411 34.1576820415596 16.741362536483148 14.507346521316137 7 30.59427837234322 30.863216917990204 19.692341685624207 18.85016302404237 8 26.90148899086435 34.91802906658201 19.11547852725394 19.065003415299696 9 26.63263030989861 16.67576142859922 19.171874540170002 37.51973372133217 10-11 25.809279503032784 25.631206899540242 26.76979913584907 21.789714461577905 12-13 26.367210515335906 22.716760216319102 27.125018190152467 23.79101107819253 14-15 23.240866435914022 24.406974051736423 24.828565845989754 27.5235936663598 16-17 23.482763539442015 27.530531263435204 25.560949309573992 23.425755887548792 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25.72800391380649 26.873308468094848 23.91582373707678 44-45 23.38648265490902 26.02604434346961 26.996583622955235 23.590889378666134 46-47 23.570309989211673 26.24415424588058 26.52573029385285 23.659805471054902 48-49 23.61451697814066 25.970265393968383 26.728134956979797 23.687082670911156 50-51 23.67436245586444 26.246566264549585 26.545561162999775 23.533510116586196 52-53 23.661728268246236 26.088264376763014 26.532069504911888 23.71793785007886 54-55 23.399837764475755 26.096856876827783 26.744315492370607 23.758989866325855 56-57 23.446532701628815 26.318684839266137 26.67944752199418 23.55533493711086 58-59 23.53682769694129 25.93448005904 26.761725723289885 23.766966520728825 60-61 23.463098175072833 26.108201638308138 26.778347206137358 23.65035298048167 62-63 23.256088190697575 26.204212981591702 26.84864549256244 23.691053335148286 64-65 23.227934303845093 26.06853982699125 26.77594252421646 23.927583344947195 66-67 23.251622704993224 26.168122595983945 26.63504878243057 23.94520591659226 68-69 23.245849302747683 25.946549702718013 26.86861937621582 23.93898161831848 70-71 23.297772254329207 26.01825214936152 26.69260271540556 23.991372880903718 72-73 23.370618362815723 25.963822728903107 26.647664886797823 24.01789402148335 74-75 23.2521355027369 26.346047753724775 26.456421282030984 23.945395461507342 76-77 23.529103038863266 26.088372809114407 26.43184607530806 23.950678076714265 78-79 23.56031502124919 26.049037239087735 26.57202416946297 23.818623570200103 80-81 23.435829993580196 26.279542012328523 26.417673786625613 23.866954207465675 82-83 23.549347785682436 26.282548544134087 26.29846700372582 23.869636666457662 84-85 23.516378779381512 26.131541068829307 26.33806849734966 24.014011654439525 86-87 23.58010361831382 26.037052628534664 26.3886508265269 23.994192926624617 88-89 23.62475683329306 26.075475753900957 26.25270285670539 24.047064556100594 90-91 23.606201490457543 26.129717064947066 26.242240187738048 24.021841256857346 92-93 23.635278046143434 25.758888825303995 26.415109865345897 24.190723263206674 94-95 23.545162061377177 25.876902754694907 26.386851798443818 24.191083385484095 96-97 23.691991664059557 25.737309642929418 26.25614250652547 24.314556186485557 98-99 23.705765452169835 25.76807033642194 26.062439532819344 24.463724678588882 100-101 24.315200215252204 25.192545145181672 26.09739563300597 24.39485900656015 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 320.0 1 240.0 2 210.0 3 290.5 4 259.0 5 259.5 6 472.0 7 980.5 8 1593.0 9 1849.5 10 1652.0 11 1478.0 12 1556.5 13 1702.5 14 1881.5 15 2049.0 16 2238.0 17 2409.0 18 2597.0 19 2846.0 20 3245.5 21 3868.5 22 4594.5 23 5659.0 24 7144.5 25 9239.5 26 12157.5 27 15631.5 28 18966.0 29 22994.0 30 28252.0 31 35375.5 32 44807.0 33 51723.0 34 56872.5 35 63467.0 36 72652.0 37 81835.5 38 88519.0 39 94973.0 40 101773.5 41 112097.0 42 122094.5 43 129186.5 44 136771.0 45 145569.0 46 152552.5 47 158886.0 48 162516.0 49 162543.0 50 162835.0 51 160472.0 52 160237.5 53 162738.0 54 163715.5 55 154831.5 56 138598.5 57 125490.0 58 115002.0 59 102233.5 60 85614.0 61 71810.5 62 60084.0 63 47918.0 64 37810.5 65 30032.0 66 23250.5 67 18338.0 68 14428.0 69 10815.0 70 8271.5 71 6157.5 72 4763.5 73 3786.5 74 2880.0 75 2245.0 76 1606.5 77 995.0 78 627.0 79 395.0 80 233.0 81 138.0 82 88.0 83 47.5 84 24.5 85 20.0 86 16.5 87 13.0 88 9.0 89 5.5 90 5.5 91 4.0 92 1.0 93 1.5 94 1.5 95 1.5 96 2.0 97 3.0 98 2.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1849779459919243 2 0.048413365379557545 3 0.046468853278833815 4 0.052427037792589865 5 0.05903339300658716 6 0.06454284395863774 7 0.036172911001924817 8 0.03470206210522353 9 0.04125855803458689 10-11 0.04209370105220541 12-13 0.039002425404901016 14-15 0.03409128586845774 16-17 0.03243346465437918 18-19 0.040386020553492906 20-21 0.050507455334183105 22-23 0.04757822236193902 24-25 0.06735989354045545 26-27 0.05318739188325748 28-29 0.044636524568536444 30-31 0.05374830883538933 32-33 0.049859284633941854 34-35 0.04898674715284788 36-37 0.046568571848101696 38-39 0.026238448538611906 40-41 0.024368725364839083 42-43 0.021713718458081677 44-45 0.004200644730409602 46-47 0.01176679117361028 48-49 0.010981507440625694 50-51 0.008750304453256795 52-53 0.010146364423007167 54-55 0.011305592790746317 56-57 0.008525937672404057 58-59 0.011193409400319947 60-61 0.011068761188735092 62-63 0.004063531697666262 64-65 0.0069304405641179186 66-67 0.004051066876507776 68-69 0.009136713909169845 70-71 0.022735833793077486 72-73 0.019632093324614606 74-75 0.020355052951806764 76-77 0.012377567410376067 78-79 0.004848815430650846 80-81 0.006581425571680325 82-83 0.005546845415526032 84-85 0.0049734636422357 86-87 0.007940091077955241 88-89 0.029416977934025697 90-91 0.03606072761149845 92-93 0.022499002191066264 94-95 0.03192240698688127 96-97 0.03548734583820812 98-99 0.021165266327108318 100-101 0.12031045382170169 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4011289.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.95941712230199 #Duplication Level Percentage of deduplicated Percentage of total 1 73.55962555962391 36.01436391133974 2 13.967918926299477 13.677223380863854 3 4.942690452552588 7.259737307188254 4 2.4146296908271885 4.728754489164137 5 1.3286484469513284 3.2524926761594415 6 0.8582954060990857 2.5212985680816433 7 0.543241263861124 1.8617742944796307 8 0.3902902030092509 1.5286704678302272 9 0.2709117381889464 1.1937312713988446 >10 1.5170762802903939 13.553742283822226 >50 0.11883982186834022 4.001207611649664 >100 0.08147374837613353 7.326549700430461 >500 0.004768846587923121 1.585805842731104 >1k 0.001538337546058611 1.3353295177030675 >5k 5.12779182019536E-5 0.15931867715775172 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6346 0.1582035101434975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4893 0.12198073985693876 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.971856926788371E-5 2 0.0 0.0 0.0 0.0 9.971856926788371E-5 3 0.0 0.0 0.0 0.0 9.971856926788371E-5 4 0.0 0.0 0.0 0.0 9.971856926788371E-5 5 0.0 0.0 0.0 0.0 9.971856926788371E-5 6 0.0 0.0 0.0 0.0 9.971856926788371E-5 7 0.0 0.0 0.0 0.0 9.971856926788371E-5 8 0.0 0.0 0.0 0.0 9.971856926788371E-5 9 0.0 0.0 0.0 0.0 9.971856926788371E-5 10-11 0.0 0.0 0.0 0.0 9.971856926788371E-5 12-13 0.0 0.0 0.0 0.0 9.971856926788371E-5 14-15 0.0 0.0 0.0 0.0 1.2464821158485463E-4 16-17 0.0 0.0 0.0 1.2464821158485464E-5 1.2464821158485463E-4 18-19 0.0 0.0 0.0 6.232410579242732E-5 1.4957785390182557E-4 20-21 0.0 0.0 0.0 8.725374810939824E-5 1.4957785390182557E-4 22-23 0.0 0.0 0.0 1.8697231737728194E-4 1.7450749621879648E-4 24-25 0.0 0.0 0.0 3.864094559130494E-4 1.9943713853576742E-4 26-27 0.0 0.0 0.0 8.226781964600406E-4 1.9943713853576742E-4 28-29 0.0 0.0 0.0 0.002330921556636782 1.9943713853576742E-4 30-31 0.0 0.0 0.0 0.008413754281977688 1.9943713853576742E-4 32-33 0.0 0.0 0.0 0.023371539672160248 1.9943713853576742E-4 34-35 0.0 0.0 0.0 0.047553292719622046 1.9943713853576742E-4 36-37 0.0 0.0 0.0 0.08983396608920474 1.9943713853576742E-4 38-39 0.0 0.0 0.0 0.15547371430978918 1.9943713853576742E-4 40-41 0.0 0.0 0.0 0.22669770240937515 2.119019596942529E-4 42-43 0.0 0.0 0.0 0.3026458577280271 2.2436678085273836E-4 44-45 0.0 0.0 0.0 0.3927291202404015 2.492964231697093E-4 46-47 0.0 0.0 0.0 0.5034915210547033 2.7422606548668023E-4 48-49 0.0 0.0 0.0 0.6209101363676365 2.9915570780365114E-4 50-51 0.0 0.0 0.0 0.747353282199313 2.9915570780365114E-4 52-53 0.0 0.0 0.0 0.8708921247010624 2.9915570780365114E-4 54-55 1.2464821158485464E-5 0.0 0.0 1.009912275081651 3.116205289621366E-4 56-57 2.4929642316970928E-5 0.0 0.0 1.1747719997237795 3.2408535012062205E-4 58-59 2.4929642316970928E-5 0.0 0.0 1.3515231637511036 3.4901499243759296E-4 60-61 2.4929642316970928E-5 0.0 0.0 1.5442542285036058 3.7394463475456393E-4 62-63 4.9859284633941855E-5 0.0 0.0 1.7375462102082397 4.238039193885058E-4 64-65 7.478892695091279E-5 0.0 0.0 1.941882023459292 4.238039193885058E-4 66-67 7.478892695091279E-5 0.0 0.0 2.1742761491380946 4.238039193885058E-4 68-69 7.478892695091279E-5 0.0 0.0 2.431450339279967 4.238039193885058E-4 70-71 7.478892695091279E-5 0.0 0.0 2.7067857738497523 4.238039193885058E-4 72-73 9.971856926788371E-5 0.0 0.0 2.9869949534925055 4.736632040224476E-4 74-75 9.971856926788371E-5 0.0 0.0 3.2767147916791837 4.736632040224476E-4 76-77 9.971856926788371E-5 0.0 0.0 3.591389201825149 4.861280251809331E-4 78-79 9.971856926788371E-5 0.0 0.0 3.940852927824448 4.985928463394185E-4 80-81 9.971856926788371E-5 0.0 0.0 4.31357601010548 4.985928463394185E-4 82-83 9.971856926788371E-5 0.0 0.0 4.6976171499984165 5.235224886563896E-4 84-85 9.971856926788371E-5 2.4929642316970928E-5 0.0 5.08103504883343 5.235224886563896E-4 86-87 9.971856926788371E-5 2.4929642316970928E-5 0.0 5.484147365098849 5.235224886563896E-4 88-89 9.971856926788371E-5 2.4929642316970928E-5 0.0 5.919730540482124 5.235224886563896E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5170 0.0 42.72374 1 GTATCAA 8295 0.0 40.401554 1 ATCAACG 10135 0.0 32.449924 3 TATCAAC 10615 0.0 31.251205 2 TCAACGC 10515 0.0 31.141624 4 CAACGCA 10590 0.0 30.83055 5 AACGCAG 11360 0.0 28.94312 6 GTACATG 11535 0.0 25.215374 1 TACATGG 11460 0.0 24.550957 2 CGCAGAG 13440 0.0 24.07255 8 ACGCAGA 13495 0.0 24.044847 7 ACATGGG 11955 0.0 22.500814 3 GCAGAGT 14935 0.0 20.547976 9 GAGTACT 7560 0.0 20.231073 12-13 TAGGTCG 285 1.9554136E-9 20.010454 5 CATGGGG 8620 0.0 19.683046 4 GTGTAAG 1675 0.0 19.609932 1 GTACTTT 7900 0.0 19.061644 14-15 TAAGGTG 1485 0.0 18.881916 5 AGAGTAC 12975 0.0 18.873018 10-11 >>END_MODULE