FastQCFastQC Report
Thu 26 May 2016
SRR938390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4011289
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66080.1647350764305439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53440.13322400854189265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54300.042.301071
GTATCAA88050.038.023091
ATCAACG108600.030.3324133
TCAACGC110350.029.8940874
TATCAAC112600.029.5218012
CAACGCA112150.029.456675
AACGCAG119250.027.7604146
TACATGG110050.024.1128332
GTACATG113800.023.8615111
ACGCAGA142850.022.9414277
CGCAGAG142150.022.8205138
ACATGGG114000.022.4326733
GCAGAGT157950.020.116769
GAGTACT81050.019.95334212-13
AGAGTAC137700.018.78085910-11
GTACTTT85900.018.66088114-15
CATGGGG82050.018.6541484
CAGAGTA147250.017.83698310-11
AGTACTT90000.017.52052512-13
GCACCGT9150.017.129496
Produced by FastQC (version 0.11.5)