##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938390_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4011289 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79269855649892 31.0 31.0 34.0 30.0 34.0 2 32.024532014522016 31.0 31.0 34.0 30.0 34.0 3 32.09823874569995 33.0 31.0 34.0 30.0 34.0 4 35.756768958806006 37.0 35.0 37.0 35.0 37.0 5 35.619555958196976 37.0 35.0 37.0 33.0 37.0 6 35.64455241195536 37.0 35.0 37.0 33.0 37.0 7 35.58150434935005 37.0 35.0 37.0 33.0 37.0 8 35.56864763421434 37.0 35.0 37.0 33.0 37.0 9 37.160492051308196 39.0 37.0 39.0 33.0 39.0 10-11 37.065437319524975 39.0 37.0 39.0 33.0 39.0 12-13 36.952986558684756 39.0 37.0 39.0 33.0 39.0 14-15 38.030218839879154 40.0 37.0 41.0 33.0 41.0 16-17 37.943120154144964 40.0 37.0 41.0 33.0 41.0 18-19 38.02651628940224 40.0 37.0 41.0 33.0 41.0 20-21 38.07784405461686 40.0 37.0 41.0 33.0 41.0 22-23 37.97661861311913 40.0 37.0 41.0 33.0 41.0 24-25 37.88040864171093 40.0 37.0 41.0 32.0 41.0 26-27 37.690917433273945 40.0 37.0 41.0 32.0 41.0 28-29 37.572340960723594 39.5 37.0 41.0 32.0 41.0 30-31 37.37055320621376 39.0 36.0 41.0 31.5 41.0 32-33 37.18588725469543 39.0 36.0 41.0 31.0 41.0 34-35 37.05518525341854 39.0 36.0 41.0 31.0 41.0 36-37 37.085252895017035 39.0 36.0 41.0 31.0 41.0 38-39 36.97757566208767 39.0 36.0 41.0 31.0 41.0 40-41 36.824588679599 39.0 35.5 41.0 30.0 41.0 42-43 36.63136861991245 39.0 35.0 40.5 30.0 41.0 44-45 36.402849183890766 39.0 35.0 40.0 30.0 41.0 46-47 36.086518199012836 38.5 35.0 40.0 29.5 41.0 48-49 35.769024370969035 38.0 35.0 40.0 28.0 41.0 50-51 35.41992997762066 38.0 34.0 40.0 27.5 41.0 52-53 34.929675847339844 38.0 33.5 40.0 26.0 41.0 54-55 34.61682691025254 38.0 33.0 40.0 26.0 41.0 56-57 34.2670130474269 37.0 33.0 40.0 25.0 41.0 58-59 33.812696616972744 37.0 32.5 39.5 24.0 41.0 60-61 33.43232848593058 36.0 32.0 39.0 23.0 40.5 62-63 32.960522789557174 36.0 31.0 39.0 22.5 40.0 64-65 32.44708384262515 35.0 31.0 38.5 21.5 40.0 66-67 31.934843388247522 35.0 30.5 38.0 20.0 40.0 68-69 31.3790762769773 34.5 30.0 37.5 18.5 39.5 70-71 30.789951808508437 34.0 29.0 37.0 17.0 39.0 72-73 30.327187470162336 34.0 29.0 36.0 13.0 39.0 74-75 29.786319808919277 34.0 28.5 36.0 9.5 38.5 76-77 28.51971972101736 31.5 27.0 34.5 8.0 36.5 78-79 29.217479468569827 33.0 28.5 35.0 7.0 37.0 80-81 28.936137735276617 33.0 28.0 35.0 6.0 36.5 82-83 28.459403947209985 33.0 27.0 35.0 2.0 36.0 84-85 27.932006893544692 33.0 26.0 35.0 2.0 36.0 86-87 27.340427104604032 32.5 26.0 35.0 2.0 35.0 88-89 26.690441027809264 32.0 25.0 34.0 2.0 35.0 90-91 26.129453774086087 32.0 24.0 34.0 2.0 35.0 92-93 25.35323520693722 31.0 21.5 34.0 2.0 35.0 94-95 24.553275892113483 31.0 18.5 34.0 2.0 35.0 96-97 23.513291363449504 31.0 4.5 34.0 2.0 35.0 98-99 22.220695018484083 30.0 2.0 34.0 2.0 35.0 100-101 18.511553393435378 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31175669653956106 1101 2 0.3184327426656708 1101 3 0.2669989943029307 1101 4 0.2109827519460552 1101 5 0.09273688562913662 1101 6 0.12702896891616433 1101 7 0.24667223438768104 1101 8 0.2571994382481364 1101 9 0.2848876997373253 1101 10-11 0.34174089058672763 1101 12-13 0.3388891600226813 1101 14-15 0.3341400500488021 1101 16-17 0.38027552417862154 1101 18-19 0.4061885050103058 1101 20-21 0.4411468329184416 1101 22-23 0.4478506649996774 1101 24-25 0.48058859026411227 1101 26-27 0.5104163005357165 1101 28-29 0.38217357086075054 1101 30-31 0.43105096794103304 1101 32-33 0.31986029120088944 1101 34-35 0.233912193620192 1101 36-37 0.3864587111238009 1101 38-39 0.4761755327657866 1101 40-41 0.4765500334956485 1101 42-43 0.5062286521270707 1101 44-45 0.43471295280134825 1101 46-47 0.4842899747410172 1101 48-49 0.5778540685335898 1101 50-51 0.597777573206514 1101 52-53 0.599344079426487 1101 54-55 0.5349613841191427 1101 56-57 0.5963277247316086 1101 58-59 0.693333018089568 1101 60-61 0.649781125156828 1101 62-63 0.576986402661575 1101 64-65 0.5599218898675034 1101 66-67 0.460096824693224 1101 68-69 0.5806035371794955 1101 70-71 0.5616335274160491 1101 72-73 0.5245263157849145 1101 74-75 0.5370908230936315 1101 76-77 0.5168434205704209 1101 78-79 0.4983369063390626 1101 80-81 0.6330728990665406 1101 82-83 0.7286604125622489 1101 84-85 0.6440990079784363 1101 86-87 0.6303939460442614 1101 88-89 0.5528809124904939 1101 90-91 0.7118181296030102 1101 92-93 0.7341799848719646 1101 94-95 0.68271719735041 1101 96-97 0.4529610656712606 1101 98-99 0.38548678458636587 1101 100-101 0.18953108659442464 1102 1 0.3136010090910162 1102 2 0.2799299411850882 1102 3 0.29664817719542924 1102 4 0.23194186199788902 1102 5 0.1639126431525142 1102 6 0.21530727651073533 1102 7 0.2869157649446734 1102 8 0.2522199361618789 1102 9 0.26964803161499873 1102 10-11 0.34525823832037617 1102 12-13 0.3703351853343193 1102 14-15 0.3874512031935282 1102 16-17 0.45613779245955044 1102 18-19 0.4044511184254276 1102 20-21 0.39249092641853167 1102 22-23 0.3782240341339076 1102 24-25 0.40009899695957785 1102 26-27 0.43497005428201163 1102 28-29 0.3962802694028582 1102 30-31 0.3575720986998405 1102 32-33 0.37801403932909494 1102 34-35 0.34390764783170624 1102 36-37 0.40939559705071105 1102 38-39 0.44802442756927263 1102 40-41 0.5114291014766579 1102 42-43 0.4610829694647052 1102 44-45 0.4856111984457101 1102 46-47 0.395283172804497 1102 48-49 0.5755199290408157 1102 50-51 0.49669887353430653 1102 52-53 0.4134489818014302 1102 54-55 0.28053214392215864 1102 56-57 0.43360399387901793 1102 58-59 0.35812416436294114 1102 60-61 0.4525641984015536 1102 62-63 0.4375179722577869 1102 64-65 0.4724111061119842 1102 66-67 0.5864127534818095 1102 68-69 0.44572485409144846 1102 70-71 0.3500212323951857 1102 72-73 0.24093788844946573 1102 74-75 0.1730626022012629 1102 76-77 0.27607450436418546 1102 78-79 0.39285239767316327 1102 80-81 0.3994165009840387 1102 82-83 0.3875111073143067 1102 84-85 0.4087220708189001 1102 86-87 0.2859307798958284 1102 88-89 0.336844813041715 1102 90-91 0.21265719626593338 1102 92-93 0.15920914895466964 1102 94-95 -0.04337456842507237 1102 96-97 -0.022386776281155818 1102 98-99 -0.0215660886328628 1102 100-101 -0.11319813539779133 1103 1 0.26585912668648604 1103 2 0.2689556467553871 1103 3 0.2794081593315525 1103 4 0.19932568563731934 1103 5 0.1617841866085783 1103 6 0.18356340138711147 1103 7 0.2549606273375957 1103 8 0.18227920709738754 1103 9 0.2817587702700024 1103 10-11 0.35189419017303436 1103 12-13 0.32858865450631214 1103 14-15 0.3845467333504402 1103 16-17 0.4394195072828353 1103 18-19 0.416668818445288 1103 20-21 0.44701270595198395 1103 22-23 0.4424236597918423 1103 24-25 0.5150439554912012 1103 26-27 0.403335930537601 1103 28-29 0.5179229169655812 1103 30-31 0.558899632955864 1103 32-33 0.7098263930844908 1103 34-35 0.5272854948710588 1103 36-37 0.46347431581383347 1103 38-39 0.4956649286376802 1103 40-41 0.58540548900109 1103 42-43 0.6390355362357525 1103 44-45 0.7234398743042405 1103 46-47 0.684818146834985 1103 48-49 0.7449895544463487 1103 50-51 0.6893834623449067 1103 52-53 0.7808637681119563 1103 54-55 0.8232961540604293 1103 56-57 0.8160887508301684 1103 58-59 0.8303217036092008 1103 60-61 0.8017458190586169 1103 62-63 0.7201879537210587 1103 64-65 0.7349044822610153 1103 66-67 0.6802119335679606 1103 68-69 0.6027335615965299 1103 70-71 0.3288979038327753 1103 72-73 0.3453046697078612 1103 74-75 0.5031257467148578 1103 76-77 0.5226586346197024 1103 78-79 0.49470754960015384 1103 80-81 0.4900495987168796 1103 82-83 0.566153845779592 1103 84-85 0.5476870092273849 1103 86-87 0.5379612452955307 1103 88-89 0.6809623305232648 1103 90-91 0.7826220946409563 1103 92-93 0.7175553893486715 1103 94-95 0.6757375555693947 1103 96-97 0.5480184252918683 1103 98-99 0.3560962906900933 1103 100-101 -0.13570625190645913 1104 1 0.251939812558831 1104 2 0.2373035358206863 1104 3 0.26421749822367957 1104 4 0.22230446352153876 1104 5 0.14440681613821482 1104 6 0.1645871706629407 1104 7 0.14695642623551208 1104 8 0.20355286900096559 1104 9 0.21423558195805725 1104 10-11 0.31053233415530457 1104 12-13 0.3173307579007698 1104 14-15 0.43189045183193286 1104 16-17 0.43396883040966117 1104 18-19 0.3941015721545682 1104 20-21 0.3674902500788164 1104 22-23 0.36667123526318335 1104 24-25 0.418181160478035 1104 26-27 0.45233359105073845 1104 28-29 0.5263926707425952 1104 30-31 0.5393156418046772 1104 32-33 0.5067330860415424 1104 34-35 0.49626031133335147 1104 36-37 0.4604672415271196 1104 38-39 0.4921047104840639 1104 40-41 0.5234582141720452 1104 42-43 0.5894296500778822 1104 44-45 0.38632339048674424 1104 46-47 0.3347994078652903 1104 48-49 0.40766353656858456 1104 50-51 0.6203120294668949 1104 52-53 0.6109083843520011 1104 54-55 0.57622265263835 1104 56-57 0.5827439173148363 1104 58-59 0.49551511880596166 1104 60-61 0.4130298981173368 1104 62-63 0.40469283552970836 1104 64-65 0.546213106206551 1104 66-67 0.4589544798496803 1104 68-69 0.34077709406537693 1104 70-71 0.20465993045513642 1104 72-73 0.0951540322107185 1104 74-75 0.15871762646985133 1104 76-77 0.260169585844789 1104 78-79 0.36153413950961877 1104 80-81 0.41687987687241446 1104 82-83 0.3893781841841708 1104 84-85 0.36882940529960706 1104 86-87 0.3868672765268961 1104 88-89 0.2767882482861701 1104 90-91 0.20556157093234617 1104 92-93 0.1680586432674751 1104 94-95 0.2759124216053017 1104 96-97 0.29470021339524877 1104 98-99 0.08098448956806337 1104 100-101 -0.11863024947392731 1105 1 0.23214488672174838 1105 2 0.2583741983042529 1105 3 0.15894833542599685 1105 4 0.1553066899950366 1105 5 0.09259325571689203 1105 6 0.13430668139740476 1105 7 0.1575285063620484 1105 8 0.18888988645438332 1105 9 0.19559579014729422 1105 10-11 0.2434418303511805 1105 12-13 0.26970278301640604 1105 14-15 0.3422318128733366 1105 16-17 0.27514500302456213 1105 18-19 0.2655764013580537 1105 20-21 0.3057363826285524 1105 22-23 0.300235465212495 1105 24-25 0.3708748714833874 1105 26-27 0.2906919941063606 1105 28-29 0.2735363178131678 1105 30-31 0.39725273812912576 1105 32-33 0.5172063998417471 1105 34-35 0.49799715475919726 1105 36-37 0.4161660171323902 1105 38-39 0.4345379671714156 1105 40-41 0.3955350504682471 1105 42-43 0.4176890927067731 1105 44-45 0.3048199267416223 1105 46-47 0.21367762833398274 1105 48-49 0.15736599487369318 1105 50-51 0.15180138542759636 1105 52-53 0.24689239521404716 1105 54-55 0.2869051924561461 1105 56-57 0.3710188572028983 1105 58-59 0.4301045893805764 1105 60-61 0.4256422218824696 1105 62-63 0.513555400145016 1105 64-65 0.44805890622918554 1105 66-67 0.32590653326610663 1105 68-69 0.22094886344192233 1105 70-71 0.2149849468234848 1105 72-73 0.19813144934050442 1105 74-75 0.23928715962053815 1105 76-77 0.19193359385687359 1105 78-79 0.3272083140740385 1105 80-81 0.2665404260628961 1105 82-83 0.3200271801407304 1105 84-85 0.4322154913315295 1105 86-87 0.5157504086822762 1105 88-89 0.3131896459983423 1105 90-91 0.32676175991984735 1105 92-93 0.3940086581563982 1105 94-95 0.5114373258688367 1105 96-97 0.2647304622840245 1105 98-99 0.14487115340370238 1105 100-101 -0.13248079170046978 1106 1 0.11068645396787957 1106 2 0.06245180510327941 1106 3 0.16442390842728827 1106 4 0.10212199603457606 1106 5 0.010791249182055651 1106 6 -0.007632233919117937 1106 7 0.09356359012174664 1106 8 0.1135037987138503 1106 9 0.02842702399016872 1106 10-11 0.05454349417775717 1106 12-13 0.08412985365502124 1106 14-15 0.16957013960850986 1106 16-17 0.01275889960079013 1106 18-19 0.19722483596443396 1106 20-21 0.1431927275069924 1106 22-23 0.16478162823729292 1106 24-25 0.23091104198543633 1106 26-27 0.06162569869615453 1106 28-29 0.24899039770170361 1106 30-31 0.2668930058950778 1106 32-33 0.18159195116832905 1106 34-35 0.04158927804691359 1106 36-37 -0.34021912477910377 1106 38-39 -0.04122544397318251 1106 40-41 0.20746167625708978 1106 42-43 0.3794619048330006 1106 44-45 0.2913479653662634 1106 46-47 0.023679283918028204 1106 48-49 0.1087823564541921 1106 50-51 0.2286101964835936 1106 52-53 0.19057553435231966 1106 54-55 -0.02370920020570111 1106 56-57 0.11854582854183349 1106 58-59 0.16577373482446234 1106 60-61 -0.23329042638810904 1106 62-63 -0.047069074599129124 1106 64-65 -0.40603782704643976 1106 66-67 -0.17980355132787196 1106 68-69 -0.7490949706218615 1106 70-71 -0.7804474866574722 1106 72-73 -0.4293149148660511 1106 74-75 -0.07769430385207698 1106 76-77 -0.050429906835489646 1106 78-79 -0.08907172537225705 1106 80-81 -0.8322006874069885 1106 82-83 -0.3757476667973414 1106 84-85 -0.210237551222761 1106 86-87 -0.08244049430792799 1106 88-89 -0.026288687017199663 1106 90-91 -0.16950896038066787 1106 92-93 -0.27820480464266595 1106 94-95 -0.2631347428011104 1106 96-97 -0.34112078915002186 1106 98-99 -0.5810905976523522 1106 100-101 -0.9017668717880483 1107 1 0.15686321244177392 1107 2 0.13504941041498597 1107 3 0.13863144911198333 1107 4 0.1285946491293828 1107 5 0.09245047450811938 1107 6 0.22312488268574526 1107 7 0.10023833581068686 1107 8 0.11936857681009627 1107 9 0.1948411136397752 1107 10-11 0.18933003604814758 1107 12-13 0.18059275994473012 1107 14-15 0.2810304409556892 1107 16-17 0.23199600857757474 1107 18-19 0.1570298069210878 1107 20-21 0.20487349803986632 1107 22-23 0.23614311216125827 1107 24-25 0.22727926846793167 1107 26-27 0.23500205015530895 1107 28-29 0.31044311743552555 1107 30-31 0.31749747820360597 1107 32-33 0.27216133635542406 1107 34-35 0.1795930573219664 1107 36-37 0.24451207389547847 1107 38-39 0.19637595042406275 1107 40-41 0.20503099896686905 1107 42-43 0.3311262367758374 1107 44-45 0.35038653073568327 1107 46-47 0.2685153962870004 1107 48-49 0.247919591228964 1107 50-51 0.2622729040162781 1107 52-53 0.4052079964971824 1107 54-55 0.30075028928064285 1107 56-57 0.1575158375382273 1107 58-59 0.033866829119247654 1107 60-61 0.0872611329716193 1107 62-63 0.24416625334394126 1107 64-65 0.39523078942873724 1107 66-67 0.3677019257638676 1107 68-69 0.3652094253886098 1107 70-71 0.23825233463675488 1107 72-73 0.3482929578950298 1107 74-75 0.29640401340840583 1107 76-77 0.1797100957014699 1107 78-79 0.3339543314538709 1107 80-81 0.4751732362147507 1107 82-83 0.34503089346719307 1107 84-85 0.4877736220615958 1107 86-87 0.3637094832570398 1107 88-89 0.24914573254360306 1107 90-91 0.38557236425540964 1107 92-93 0.1712315140219225 1107 94-95 0.09056309660259032 1107 96-97 0.23900429972524861 1107 98-99 0.090615647445631 1107 100-101 -0.2582946925225649 1108 1 0.13154918900824342 1108 2 0.1415618056116017 1108 3 0.0732797141380388 1108 4 0.13112109030026176 1108 5 0.08792873595417205 1108 6 0.061386627073765965 1108 7 0.06672832286081132 1108 8 0.07246323244505959 1108 9 0.14374987668357164 1108 10-11 0.08426160170136399 1108 12-13 0.18544556560377856 1108 14-15 0.2222480054951319 1108 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-0.6174077915207405 2316 72-73 -0.4759192027381829 2316 74-75 -0.31064920900988113 2316 76-77 -0.44013535361193235 2316 78-79 -0.5193811736534393 2316 80-81 -0.5965612477918221 2316 82-83 -0.38507047766946556 2316 84-85 -0.2813471213990191 2316 86-87 -0.16620853811341973 2316 88-89 -0.05982308793236513 2316 90-91 -0.1937561283460738 2316 92-93 -0.1967024989665731 2316 94-95 -0.198567964174444 2316 96-97 -0.08599900714257913 2316 98-99 0.05397880458448867 2316 100-101 0.06786725520618475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 25.0 4 64.0 5 102.0 6 256.0 7 672.0 8 1464.0 9 2662.0 10 4356.0 11 6635.0 12 9446.0 13 13168.0 14 17298.0 15 22212.0 16 26999.0 17 32782.0 18 33627.0 19 32042.0 20 33568.0 21 36090.0 22 39778.0 23 45035.0 24 52657.0 25 61827.0 26 73952.0 27 89175.0 28 107064.0 29 127446.0 30 150983.0 31 180313.0 32 217221.0 33 267841.0 34 338302.0 35 438933.0 36 580695.0 37 651869.0 38 303341.0 39 11375.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30740945072495 18.776202742822782 14.544674017125253 24.371713789327018 2 16.406434723442974 19.978914403552476 38.680047648359896 24.934603224644658 3 19.850336191373426 22.894893047241798 28.90109010716591 28.353680654218866 4 13.918568320557306 16.251559037506397 34.961953626377955 34.86791901555834 5 14.689272726138569 36.360973358035324 33.75022300403591 15.199530911790207 6 34.56258075646008 34.699868296699634 16.43162584396188 14.3059251028784 7 30.539061134712558 30.958976029899617 19.698256595323848 18.80370624006398 8 26.832397266813736 35.43289949938785 18.87373360533235 18.860969628466062 9 26.33896485643393 16.67498901226015 19.191636404158363 37.79440972714756 10-11 25.7589767279296 25.842129549877857 26.743523091953737 21.655370630238806 12-13 26.256846614641827 22.794705143409015 27.196619341064682 23.75182890088448 14-15 23.063671054366814 24.54258469035764 24.846664002518892 27.547080252756658 16-17 23.35948619009999 27.7208523245271 25.589081215539444 23.33058026983346 18-19 23.312518743974817 26.622813763854957 27.213085369815037 22.851582122355182 20-21 23.672590032779986 25.858433535953157 27.42772959016416 23.041246841102698 22-23 23.542731525950884 25.597644547675323 27.289008595491378 23.570615330882415 24-25 23.556915831199454 25.9084478926108 27.24205013438982 23.29258614179993 26-27 23.18571243006916 26.176579628599573 27.280912866277546 23.35679507505372 28-29 23.216726593371856 26.354795179305206 27.300825245949618 23.12765298137332 30-31 23.29247531155197 26.02276973810663 27.1503748545667 23.534380095774697 32-33 23.204162053644104 25.89648863495001 27.260863029315512 23.63848628209037 34-35 23.669064380492387 26.07090868496319 27.018791226613814 23.241235707930606 36-37 23.77197317815437 25.685750131036645 27.062516788583203 23.479759902225783 38-39 23.240894630499277 25.983328548704183 27.14588747723977 23.629889343556776 40-41 23.623786823486352 25.855490604605613 27.07089221530164 23.449830356606395 42-43 23.487796709001092 25.75670151718612 26.95494910657448 23.800552667238307 44-45 23.410068434361126 26.01008055016729 27.12246611999285 23.457384895478736 46-47 23.593425953602445 26.275955185477784 26.600476804338953 23.530142056580814 48-49 23.59478461910872 25.98421604626343 26.842406518203997 23.57859281642385 50-51 23.64719919208015 26.20860775675849 26.659958930907244 23.484234120254115 52-53 23.702131658925595 26.136935035097196 26.56497699367959 23.595956312297616 54-55 23.43736639269821 26.094928587793103 26.76127299728342 23.706432022225275 56-57 23.456724259957333 26.332719981033527 26.689026893848833 23.521528865160303 58-59 23.54564829410197 26.007462937724007 26.724638887898628 23.722249880275392 60-61 23.439709779076004 26.16152812724289 26.738848784019304 23.659913309661807 62-63 23.20887375604201 26.2918353676337 26.787386797610445 23.71190407871385 64-65 23.128737420814108 26.145448507948444 26.746552043495246 23.979262027742205 66-67 23.203815035400737 26.184205491180947 26.65116291244717 23.96081656097115 68-69 23.195090612561557 26.011379483822267 26.814582173056106 23.97894773056007 70-71 23.226073440306536 26.049894235887006 26.664585039457428 24.05944728434903 72-73 23.266959822640555 26.00902104036882 26.65547159529019 24.068547541700436 74-75 23.111897447428994 26.38958449515854 26.336958020227414 24.161560037185055 76-77 23.282989582650366 26.14694428648746 26.54619250819375 24.023873622668425 78-79 23.27595942351698 26.10698206985336 26.66913303927989 23.947925467349773 80-81 23.147314725863346 26.337738331939125 26.52185852324662 23.993088418950904 82-83 23.21593175013191 26.275182078052524 26.466904854471558 24.04198131734401 84-85 23.193396308734034 26.16728869210115 26.423977023701788 24.21533797546303 86-87 23.23390642386844 26.09799625025351 26.498187763800125 24.169909562077923 88-89 23.14593414006557 26.204729132344234 26.296046491829117 24.35329023576108 90-91 23.13157234410329 26.18527969433384 26.421756625586003 24.261391335976864 92-93 23.093732510149568 25.87140433604944 26.523232753838887 24.511630399962105 94-95 22.938074488312605 25.95372756923152 26.5434136226104 24.564784319845476 96-97 22.966719675396114 25.86182396730826 26.437848282684197 24.73360807461143 98-99 22.921385120842704 25.928473366042688 26.29663432377971 24.8535071893349 100-101 23.003157214944775 25.635565729066855 26.471930403410276 24.88934665257809 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 315.0 1 226.5 2 191.0 3 285.5 4 259.5 5 244.5 6 461.0 7 1043.5 8 1580.0 9 1776.5 10 1610.0 11 1413.5 12 1514.0 13 1670.0 14 1846.5 15 2030.5 16 2200.0 17 2392.0 18 2653.0 19 2897.5 20 3198.5 21 3771.5 22 4627.5 23 5660.5 24 7208.0 25 9426.0 26 12463.0 27 15850.0 28 18974.5 29 23279.5 30 28739.5 31 35583.5 32 45266.0 33 52363.5 34 57297.5 35 63808.0 36 72997.5 37 82458.5 38 89248.5 39 95568.0 40 102193.5 41 112840.0 42 122743.5 43 130433.5 44 138959.5 45 148116.5 46 154684.5 47 161161.0 48 164375.5 49 164256.5 50 165237.5 51 162001.0 52 160971.0 53 163543.0 54 165560.0 55 156872.0 56 139254.5 57 124511.0 58 113590.5 59 100606.5 60 83270.5 61 69140.0 62 57693.5 63 45451.5 64 35270.5 65 27202.5 66 20316.0 67 16306.0 68 12926.5 69 9508.0 70 7270.0 71 5351.0 72 4130.0 73 3333.0 74 2598.0 75 2047.0 76 1400.5 77 789.5 78 473.5 79 314.0 80 174.5 81 81.5 82 40.5 83 19.0 84 11.5 85 9.5 86 4.0 87 1.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.985928463394185E-4 2 0.047790124321633265 3 7.977485541430697E-4 4 0.0 5 0.08760276310183585 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.617612443281948E-4 26-27 0.009024530518743476 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011330522433063287 36-37 1.6204267506031103E-4 38-39 0.004499800438213253 40-41 0.0056839584482693715 42-43 3.614798135960785E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.232410579242732E-5 68-69 8.725374810939825E-4 70-71 9.971856926788371E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001258946937007032 82-83 7.104948060336714E-4 84-85 0.004960998821077215 86-87 0.0021065547757840434 88-89 8.725374810939824E-5 90-91 0.0028793736876101426 92-93 8.476078387770116E-4 94-95 4.9859284633941855E-5 96-97 0.0 98-99 0.0 100-101 1.2464821158485464E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4011289.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.93447563568515 #Duplication Level Percentage of deduplicated Percentage of total 1 71.02813974838081 32.62640354720038 2 14.901793812770414 13.690121696414126 3 5.637216779015123 7.76827790366237 4 2.715668821735704 4.9897129330643395 5 1.4755958477453373 3.3890360758188187 6 0.9253389791280492 2.5502976474904284 7 0.621043721165486 1.9969122374999884 8 0.43810534799916473 1.6099311546824795 9 0.32969217894244274 1.3629813624816827 >10 1.6836874288522898 14.101370002731278 >50 0.1439430943711138 4.578340015800716 >100 0.0922509479267763 7.8743949699545555 >500 0.00561521071217738 1.7840112972279942 >1k 0.0017990480404145286 1.378908155729685 >5k 1.0903321457057718E-4 0.2993010002411951 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6608 0.1647350764305439 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5344 0.13322400854189265 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4929642316970928E-5 2 0.0 0.0 0.0 0.0 2.4929642316970928E-5 3 0.0 0.0 0.0 0.0 2.4929642316970928E-5 4 0.0 0.0 0.0 0.0 2.4929642316970928E-5 5 0.0 0.0 0.0 0.0 2.4929642316970928E-5 6 0.0 0.0 0.0 0.0 2.4929642316970928E-5 7 0.0 0.0 0.0 0.0 4.9859284633941855E-5 8 0.0 0.0 0.0 0.0 4.9859284633941855E-5 9 0.0 0.0 0.0 0.0 4.9859284633941855E-5 10-11 0.0 0.0 0.0 0.0 4.9859284633941855E-5 12-13 0.0 0.0 0.0 0.0 4.9859284633941855E-5 14-15 0.0 0.0 0.0 0.0 4.9859284633941855E-5 16-17 0.0 0.0 0.0 1.2464821158485464E-5 4.9859284633941855E-5 18-19 0.0 0.0 0.0 6.232410579242732E-5 4.9859284633941855E-5 20-21 0.0 0.0 0.0 1.1218339042636918E-4 4.9859284633941855E-5 22-23 0.0 0.0 0.0 2.119019596942529E-4 6.232410579242732E-5 24-25 0.0 0.0 0.0 4.113390982300203E-4 7.478892695091279E-5 26-27 0.0 0.0 0.0 8.476078387770116E-4 7.478892695091279E-5 28-29 0.0 0.0 0.0 0.002393245662429209 7.478892695091279E-5 30-31 0.0 0.0 0.0 0.008550867314721029 8.725374810939824E-5 32-33 0.0 0.0 0.0 0.02354604716837904 9.971856926788371E-5 34-35 0.0 0.0 0.0 0.04787737806974267 9.971856926788371E-5 36-37 0.0 0.0 0.0 0.09039488304133658 9.971856926788371E-5 38-39 0.0 0.0 0.0 0.15627146286393226 9.971856926788371E-5 40-41 0.0 0.0 0.0 0.2276824232808955 9.971856926788371E-5 42-43 0.0 0.0 0.0 0.3035807193149135 9.971856926788371E-5 44-45 0.0 0.0 0.0 0.39390081342929917 1.1218339042636918E-4 46-47 0.0 0.0 0.0 0.5051867367322573 1.2464821158485463E-4 48-49 0.0 0.0 0.0 0.6229419022164695 1.4957785390182557E-4 50-51 0.0 0.0 0.0 0.7489487793075992 1.4957785390182557E-4 52-53 0.0 0.0 0.0 0.8724751569881901 1.7450749621879648E-4 54-55 0.0 0.0 0.0 1.0122058021748122 1.9943713853576742E-4 56-57 0.0 2.4929642316970928E-5 0.0 1.1771029212804163 2.119019596942529E-4 58-59 0.0 2.4929642316970928E-5 0.0 1.3540410576251176 2.368316020112238E-4 60-61 0.0 2.4929642316970928E-5 0.0 1.5469217002315216 2.4929642316970927E-4 62-63 0.0 2.4929642316970928E-5 0.0 1.7408244581729213 2.7422606548668023E-4 64-65 0.0 2.4929642316970928E-5 0.0 1.9457959773030566 2.7422606548668023E-4 66-67 0.0 2.4929642316970928E-5 0.0 2.17851418833198 2.9915570780365114E-4 68-69 0.0 2.4929642316970928E-5 0.0 2.436012463823973 3.2408535012062205E-4 70-71 0.0 2.4929642316970928E-5 0.0 2.714040299763991 3.9887427707153484E-4 72-73 0.0 2.4929642316970928E-5 0.0 2.997652375583011 5.235224886563896E-4 74-75 0.0 2.4929642316970928E-5 0.0 3.292046521704121 5.235224886563896E-4 76-77 0.0 2.4929642316970928E-5 0.0 3.6121181994117104 5.235224886563896E-4 78-79 0.0 4.9859284633941855E-5 0.0 3.968799056861772 5.235224886563896E-4 80-81 0.0 4.9859284633941855E-5 0.0 4.34814095917796 5.359873098148751E-4 82-83 0.0 4.9859284633941855E-5 0.0 4.741094446199215 5.733817732903314E-4 84-85 0.0 4.9859284633941855E-5 0.0 5.133836031260774 5.733817732903314E-4 86-87 0.0 4.9859284633941855E-5 0.0 5.547393867657005 5.733817732903314E-4 88-89 0.0 4.9859284633941855E-5 0.0 5.998458350919119 5.733817732903314E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5430 0.0 42.30107 1 GTATCAA 8805 0.0 38.02309 1 ATCAACG 10860 0.0 30.332413 3 TCAACGC 11035 0.0 29.894087 4 TATCAAC 11260 0.0 29.521801 2 CAACGCA 11215 0.0 29.45667 5 AACGCAG 11925 0.0 27.760414 6 TACATGG 11005 0.0 24.112833 2 GTACATG 11380 0.0 23.861511 1 ACGCAGA 14285 0.0 22.941427 7 CGCAGAG 14215 0.0 22.820513 8 ACATGGG 11400 0.0 22.432673 3 GCAGAGT 15795 0.0 20.11676 9 GAGTACT 8105 0.0 19.953342 12-13 AGAGTAC 13770 0.0 18.780859 10-11 GTACTTT 8590 0.0 18.660881 14-15 CATGGGG 8205 0.0 18.654148 4 CAGAGTA 14725 0.0 17.836983 10-11 AGTACTT 9000 0.0 17.520525 12-13 GCACCGT 915 0.0 17.12949 6 >>END_MODULE